Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, Laboratório de Ecologia Isotópica, Piracicaba, São Paulo, Brazil.

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1 Marine and Freshwater Research, 2018, 69, CSIRO 2018 Supplementary material Comparison of microbial processing of Brachiaria brizantha, a C4 invasive species and a rainforest species in tropical streams of the Atlantic Forest of south-eastern Brazil A. F. Figueiredo A,C, F. G. Augusto A, L. D. Coletta A, P. J. Duarte-Neto B, E. A. Mazzi A and L. A. Martinelli A A Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, Laboratório de Ecologia Isotópica, Piracicaba, São Paulo, Brazil. B Departamento de Estatística e Informática, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil. C Corresponding author. alinefigueiredo89@gmail.com Page 1 of 6

2 Table S1. Values of degrees of freedom (d.f.), sum of squares (SS), F statistics (F) and probability (P) generated by general linear models that were used to test differences between chemical parameters of streams during the summer (n = 7) and winter (n = 7) experiments DIC, dissolved inorganic carbon; DOC, dissolved organic carbon; N, nitrogen Parameter Summer Winter d.f. SS F P d.f. SS F P Electrical conductivity Intercept Stream Error Total ph Intercept Stream Error Total O 2 (mg L 1 ) Intercept Stream Error Total O 2 (%) Intercept Stream Error Total NO 3 Intercept Stream Error Organic N Intercept Stream Error Total N Intercept Stream Error DOC Intercept Stream Error DIC Intercept Stream Error Page 2 of 6

3 Table S2. Values of degrees of freedom (d.f.), sum of squares (SS), F statistics (F) and probability (P) generated by general linear models that were used to test differences between species initial concentrations of carbon, nitrogen and the C : N ratio in the summer and winter litter-bag experiments Species refers to plants species used in the litter bag expriments: Brachiaria brizantha and Mollinedia schottiana Parameter d.f. SS F P Summer Carbon Intercept Species Error Nitrogen Intercept Species Error C : N Intercept Species Error Winter Carbon Intercept Species Error Nitrogen Intercept Species Error C : N Intercept Species Error Page 3 of 6

4 Table S3. Values of degrees of freedom (d.f.), sum of squares (SS), F statistics (F) and probability (P) generated by general linear models that were used to test differences between species initial concentrations of lignin, cellulose and fibres in the summer and winter litter-bag experiments Species refers to plants species used in the litter bag expriments: Brachiaria brizantha and Mollinedia schottiana Parameter d.f. SS MS F P Summer Lignin Intercept Species Error Cellulose Intercept Species Error Fibre Intercept Species Error Winter Lignin Intercept Species Error Cellulose Intercept Species Error Fibres Intercept Species Error Page 4 of 6

5 Table S4. Values of degrees of freedom (d.f.), sum of squares (SS), F statistics (F) and probability (P) generated by general linear models that were used to test differences in remaining mass during the summer and winter litter-bag experiments Time refers to period of time expressed in days after the beginning of the decomposition experiments. Species refers to plants species used in the litter-bag experiments, namely, Brachiaria brizantha and Mollinedia schottiana Parameter d.f. SS MS F P Season Time <0.01 Species Time <0.01 Season Species Time <0.01 Error Page 5 of 6

6 Table S5. Values of degrees of freedom (d.f.), sum of squares (SS), F statistics (F) and probability (P) generated by interactions of general linear models used to test differences between relative carbon and nitrogen concentrations and the C : N ratio, and relative lignin, cellulose and fibre concentration differences in changeover time between species and between seasons Time refers to period of time expressed in days after the beginning of the decomposition experiments. Species refers to plant species used in the litter bag, namely, Brachiaria brizantha and Mollinedia schottiana Parameter d.f. SS MS F P Carbon Season Time Species Time <0.01 Season Species Time Error Nitrogen Season Time Species Time <0.01 Season Species Time Error C : N Season Time Species Time <0.01 Season Species Time Error Lignin Season Time Species Time <0.01 Season Species Time Error Cellulose Season Time <0.01 Species Time <0.01 Season Species Time Error Fibre Season Time Species Time Season Species Time Error Page 6 of 6

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