16S Metagenomics Report

Transcription

1 Sample: BL-09 Report Date: 05/11/ :40:20

2 Sample Information Sample ID: Sample Name: Run Folder: Taxonomy File: BL_09 BL_09 D:\Illumina\MiSeqAnalysis\170509_M01675_0059_ AW8GB gg_13_5_species_32bp.dat Sequencing Statistics Total Reads Reads Passing Quality Filtering 385, , % % Reads Passing Quality Filtering Classification Rate Summary Taxonomic Level Reads Classified to Taxonomic Level Kingdom 350, % Phylum 349, % Class 347, % Order 347, % Family 345, % Genus 344, % Species 245, % % Total Reads Classified to Taxonomic Level Page 2

3 Classification Results by Taxonomic Level Tables show the highest 8 taxonomic classifications at each level. Pie charts show all classifications above 3.5% abundance. Top Kingdom Classification Results Bacteria 350, % Unclassified at Kingdom level % Viruses % Page 3

4 Top Phylum Classification Results Firmicutes 165, % Bacteroidetes 117, % Actinobacteria 31, % Proteobacteria 24, % Fusobacteria 7, % Unclassified at Phylum level 1, % Verrucomicrobia 1, % Spirochaetes % Total Phylum-level Taxonomic Categories Identified: 17. This table shows the top 8 of 17 classifications. Page 4

5 Top Class Classification Results Bacteroidia 116, % Clostridia 115, % Bacilli 48, % Actinobacteria 31, % Epsilonproteobacteria 16, % Fusobacteria 7, % Betaproteobacteria 4, % Gammaproteobacteria 3, % Total Class-level Taxonomic Categories Identified: 33. This table shows the top 8 of 33 classifications. Page 5

6 Top Order Classification Results Bacteroidales 116, % Clostridiales 88, % Lactobacillales 43, % Actinomycetales 30, % Coriobacteriales 26, % Campylobacterales 16, % Fusobacteriales 7, % Bacillales 4, % Total Order-level Taxonomic Categories Identified: 71. This table shows the top 8 of 71 classifications. Page 6

7 Top Family Classification Results Prevotellaceae 98, % Veillonellaceae 82, % Streptococcaceae 40, % Actinomycetaceae 27, % Coriobacteriaceae 26, % Paraprevotellaceae 16, % Campylobacteraceae 16, % Fusobacteriaceae 5, % Total Family-level Taxonomic Categories Identified: 156. This table shows the top 8 of 156 classifications. Page 7

8 Top Genus Classification Results Prevotella 114, % Veillonella 58, % Streptococcus 40, % Atopobium 26, % Actinomyces 26, % Megasphaera 20, % Campylobacter 16, % Unclassified at Genus level 5, % Total Genus-level Taxonomic Categories Identified: 323. This table shows the top 8 of 323 classifications. Page 8

9 Top Species Classification Results Unclassified at Species level 104, % Prevotella melaninogenica 51, % Veillonella dispar 34, % Megasphaera micronuciformis 19, % Veillonella atypica 16, % Prevotella salivae 16, % Campylobacter concisus 14, % Prevotella pallens 11, % Total Species-level Taxonomic Categories Identified: 488. This table shows the top 8 of 488 classifications. Page 9