ppendix E - Growth Rates of Individual Genes on Various Non-Fementable Carbon So ORF Gene Lactate Glycerol EtOH Description

Transcription

1 ppendix E - Growth Rates of Individual Genes on Various Non-Fementable Carbon So ORF Gene Lactate Glycerol EtOH Description YAL1C MDM1 2 2 Mitochondrial outer membrane protein involved in mitochondrial morphology and inheritance Protein of unknown function, component of the Swr1p complex th incorporates Htz1p into chromatin; required for formation of nucle YAL11W SWC associated array of smooth endoplasmic reticulum known as karm DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved i YAL15C NTG1 base excision repair, localizes to the nucleus and mitochondrion Cytochrome c heme lyase (holocytochrome c synthase), attaches to apo-cyc1p in the mitochondrial intermembrane space; human ortholog may have a role in microphthalmia with linear skin defect YAL9C CYC (MLS) H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,1-methylene-THF; expression is regulated by levels of levels of 5,1-methylene-THF YALC GCV the cytoplasm one of 2 acetyl-coa synthetases in yeast; inducible acetyl-coenzym YAL5C ACS1 5 synthetase YAR5W YAT1 Outer carnitine acetyltransferase, mitochondrial Involved in assembly of cortical actin cytoskeleton, contains SH domains, interacts with Bee1p; contains SH domains, interacts YBL7C SLA1 Bee1p Methionyl-tRNA formyltransferase, catalyzes the formylation of ini YBL1W FMT1 5 Met-tRNA in mitochondria; potential Cdc28p substrate

2 Subunit of the heme-activated, glucose-repressed Hap2p/p/p/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences YBL21C HAP contributing to both complex assembly and DNA binding YBL22C PIM1 Also known as: Lon YBLC RIB1 First step in the riboflavin biosynthesis pathway; GTP cyclohydrola YBL8W MRPL16 Mitochondrial ribosomal protein of the large subunit Core subunit of the ubiquinol-cytochrome c reductase complex (bc complex), which is a component of the mitochondrial inner memb YBL5C COR1 electron transport chain Protein required for mitochondrial translation; mutation is function YBL8C PET112 2 complemented by a Bacillus subtilis ortholog Putative transporter, member of a family of seven S. cerevisiae ge YBL89W AVT5 2 (AVT1-7) related to vesicular GABA-glycine transporters Mitochondrial ribosomal protein of the large subunit; MRP21 exhib genetic interactions with mutations in the COX2 and COX mrna YBL9W MRP21 untranslated leader sequences Alpha subunit of the F1 sector of mitochondrial F1F ATP synthase which is a large, evolutionarily conserved enzyme complex require YBL99W ATP1 ATP synthesis YBL1C Dubious open reading frame Hexaprenyl pyrophosphate synthetase, catalyzes the first step in YBRW COQ1 ubiquinone (coenzyme Q) biosynthesis Originally identified as a multicopy suppressor of a respiratory defective mutant; homolog of Sco1p; SCO1 protein homolog (S. YBR2W SCO2 5 cerevisiae)

3 YBR26C ETR enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, wh has a probable role in fatty acid synthesis YBR5C PDX 5 pyridoxine (pyridoxiamine) phosphate oxidase YBR7C SCO1 inner mitochondrial membrane protein YBRC TCM mitochondrial protein; (putative) chaperone YBR8W MIS1 2 mitochondrial C1-tetrahydroflate synthase YBR85W AAC 5 Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed und anaerobic conditions; similar to Pet9p and Aac1p; has roles in maintenance of viability and in respiration YBR1W YMC2 5 Putative mitochondrial inner membrane transporter, member of th mitochondrial carrier (MCF) family YBR115C LYS2 5 aminoadipate-semialdehyde dehydrogenase YBR12C CBP6 Translational activator of COB mrna YBR122C MRPL6 Mitochondrial Ribosomal Protein, Large subunit YBR127C VMA2 1 2 vacuolar H-ATPase regulatory subunit (subunit b), 6 kda subunit V1 sector; vacuolar ATPase V1 domain subunit B (6 kda) YBR16W MRPS9 5 Mitochondrial ribosomal protein of the small subunit YBR16W DEM1 Protein of unknown function, shows similarity to RNA-processing protein Pta1p YBR179C FZO1 Mitochondrial integral membrane protein involved in mitochondria fusion and maintenance of the mitochondrial genome; contains N- terminal GTPase domain YBR185C MBA involved in assembly of mitochondrial respiratory complexes YBR187W Hypothetical ORF YBR221C PDB1 beta subunit of pyruvate dehydrogenase (E1 beta) YBR227C MCX1 Mitochondrial ATP-binding protein, similar to ClpX YBR251W MRPS5 Mitochondrial ribosomal protein of the small subunit

4 YBR26W SHM1 Serine hydroxymethyltransferase, mitochondrial YBR268W MRPL7 YBR269C Mitochondrial Ribosomal Protein, Large subunit Also known as: Ym The authentic, non-tagged protein was localized to the mitochond YBR282W MRPL27 Mitochondrial Ribosomal Protein, Large subunit Also known as: Ym Involved in global regulation of transcription; subunit of the chrom YBR289W SNF5 remodeling Snf/Swi complex Mitochondrial inner membrane citrate transporter, member of the YBR291C CTP1 5 mitochondrial carrier family Dubious open reading frame that overlaps YCL5W-A (87%); mutations in YCL7C were thought to confer sensitivity to calcofl white, but this phenotype was later shown to be due to the defect YCL7C CWH6 1 2 YCL5W-A YCL8C STP22 2 STF2 - ATPase stabilizing factor Regulatory subunit of acetolactate synthase, which catalyzes the f step of branched-chain amino acid biosynthesis; enhances activity YCL9C ILV6 5 the Ilv2p catalytic subunit, localizes to mitochondria Zinc metalloendopeptidase, found in the cytoplasm and intermem YCL57W PRD1 space of mitochondria catabolism of hydroxy amino acids; catabolic serine (threonine) YCL6C CHA1 dehydratase Also known as: YmL2 Mitochondrial Ribosomal Protein, Large YCRW MRPL2 subunit BAR adaptor protein, subunit of a complex (Rvs161p, Rvs167p) th regulates actin, endocytosis, and viability following starvation or YCR9C RVS161 osmotic stress YCR2C 2 Hypothetical ORF

5 YCR28C-A RIM1 Single-stranded DNA-binding protein that is essential for mitochon genome maintenance; Single-stranded zinc finger DNA-binding pr YCRC PER1 Protein Processing in the ER YCR6C IMG1 Mitochondrial ribosomal protein of the small subunit, required for respiration and for maintenance of the mitochondrial genome YCR65W HCM1 Forkhead transcription factor involved in cell cycle specific transcr of SPC11, encoding a spindle pole body (SPB) calmodulin binding protein; dosage-dependent suppressor of calmodulin mutants with specific defects in SPB assembly YCR71C IMG2 2 2 Mitochondrial ribosomal protein of the small subunit YCR81W SRB8 activation mediator subcomplex of RNA polymerase I holoenzyme polymerase II mediator subunit YCR8W TRX 2 mitochondrial thioredoxin YDL6W PTC1 serine-threonine protein phosphatase YDL2W Hypothetical ORF YDLC SLM Mitochondrial protein with a potential role in protein synthesis; the bacterial homolog is responsible for the 2-thiolation of mnm5s2u trnalys, trnaglu, and trnagln YDL6C Hypothetical ORF YDLC MTF2 5 Mitochondrial matrix protein that interacts with an N-terminal regi mitochondrial RNA polymerase (Rpo1p) and couples RNA process and translation to transcription YDL5W-A MRP1 Mitochondrial ribosomal protein of the small subunit YDL56W MBP1 transcription factor YDL66W IDP1 Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes th oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alphaketoglutarate to biosynthetic processes

6 YDL67C COX9 Subunit VIIa of cytochrome c oxidase, which is the terminal memb the mitochondrial inner membrane electron transport chain YDL68W 2 Hypothetical ORF YDL69C CBS1 translational activator of cytochrome b YDL85W NDE2 NADH DEhydrogenas YDL1C QRI7 5 similar to H.influenzae sialoglycoprotease YDL16C PHO2 Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Phop the PHO5 promoter; phosphorylation of Pho2p facilitates interactio with Phop YDL17W MSS2 2 cox1 pre-mrna splicing factor YDL118W Hypothetical ORF YDL119C 2 Hypothetical ORF YDL1W-A STF1 2 ATPase stabilizing factor YDL12C CRD1 Cardiolipin synthase; produces cardiolipin, which is an important constituent of mitochondrial membranes; required for normal mitochondrial membrane potential and function YDL17C DLD1 YDL181W INH1 D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcrip is heme-dependent, repressed by glucose, and derepressed in eth or lactate; located in the mitochondrial inner membrane Protein that inhibits ATP hydrolysis by the F1F-ATP synthase, inhibitory function is enhanced by stabilizing proteins Stf1p and St has similarity to Stf1p and both Inh1p and Stf1p exhibit the poten to form coiled-coil structures

7 YDL185W TFP1 YDL198C GGC1 2 YDL22W MRPL11 5 Encodes a protein with three regions (ABC) that is spliced to yield extein AC & the intein B; AC is a 69K vacuolar (H+)-ATPase & B is 5K site-specific endonuclease named VDE (PI-SceI) that is homologous to HO; vacuolar ATPase V1 domain subunit A (69 kda Mitochondrial GTP/GDP transporter, essential for mitochondrial ge maintenance; has a role in mitochondrial iron transport; member the mitochondrial carrier family; (putative) mitochondrial carrier protein Also known as: YmL11 Mitochondrial Ribosomal Protein, Large subunit T subunit of the mitochondrial glycine decarboxylase complex, req for the catabolism of glycine to 5,1-methylene-THF; expression i YDR19C GCV1 2 regulated by levels of levels of 5,1-methylene-THF in the cytopla -deoxy-d-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is YDR5W ARO feedback-inhibited by phenylalanine YDR65W Hypothetical ORF Ubiquitin hydrolase, required for recycling ubiquitin from proteaso bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from YDR69C DOA 5 ubiquitinated membrane proteins en route to the vacuole YDR7W TPS Trehalose-6-phosphate phosphatase Chaperone that specifically facilitates the assembly of cytochrome YDR79W PET1 oxidase, located in the mitochondrial inner membrane YDR11C Hypothetical ORF

8 YDR115W Putative mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins YDR116C MRPL1 1 2 Mitochondrial ribosomal protein of the large subunit YDR12C TRM1 trna methyltransferase, localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguano in trnas in both compartments YDR129C SAC6 2 fimbrin homolog (actin-filament bundling protein) YDR16C VPS61 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; % of ORF overlaps the verified gene RGP1; deletion causes a vacuolar prote sorting defect YDR18C KGD2 1 2 dihydrolipoyl transsuccinylase component of alpha-ketoglutarate dehydrogenase complex in mitochondria YDR15W NUM1 Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex YDR162C NBP interacts with Nap1, which is involved in histone assembly YDR175C RSM2 Mitochondrial ribosomal protein of the small subunit YDR178W SDH Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sd Sdhp, Sdhp), which couples the oxidation of succinate to the transfer of electrons to ubiquinone YDR19C MSS116 Mitochondrial RNA helicase of the DEAD box family, necessary for splicing of several mitochondrial introns YDR195W REF2 RNA-binding protein involved in cleavage step of mrna '-end formation, prior to polyadenylation; RNA-binding protein, involved mrna processing YDR197W CBS2 1 Translational activator of COB mrna; soluble protein; cytochrome translational activator

9 Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, poss functioning in stabilization of Coq7p; located on the matrix face of YDR2W COQ mitochondrial inner membrane YDR225W YDR2W HTA1 Histone H2A (HTA1 and HTA2 code for nearly identical proteins) Hypothetical ORF COX2 - in the maturation and assembly of cytochrome oxidase YDR21C COX2 involved protein YDR2W LYS 2 homoaconitase YDR27W MRPL7 2 Also known as: YmL5/7 Mitochondrial Ribosomal Protein, Large su Oligomeric mitochondrial matrix chaperone that cooperates with S in mitochondrial thermotolerance after heat shock; prevents the aggregation of misfolded matrix proteins; component of the YDR258C HSP78 2 mitochondrial proteolysis system YDR268W YDR269C YDR27W YDR271C MSW1 CCC2 5 mitochondrial tryptophanyl-trna synthetase Hypothetical ORF CCA1 - trna nucleotidyltransferase Hypothetical ORF plasma membrane protein involved in salt tolerance; hypothetical YDR276C PMP transmembrane protein Protein with a role in maintenance of cellular integrity, interacts w components of the TOR pathway; ssd1 mutant of a clinical S. YDR29C SSD1 cerevisiae strain displays elevated virulence YDR296W MHR1 2 Protein involved in mitochondrial homologous DNA recombination in transcription regulation; binds to activation domains of acidic activators; presence in RNA pol II holoenzyme may help recruit an Ssnp-active form of the holoenzyme to target promoters

10 Subunit 5 of the stator stalk of mitochondrial F1F ATP synthase, which is a large, evolutionarily conserved enzyme complex require ATP synthesis; homologous to bovine subunit OSCP (oligomycin YDR298C ATP5 sensitivity-conferring protein) YDR16W OMS1 5 OXA1 Multicopy Suppressor YDR22W MRPL5 Also known as: YmL5 Mitochondrial Ribosomal Protein, Large sub vacuolar segregation protein required for vacuole inheritance and vacuole protein sorting; cytosolic and peripheral membrane protei with three zinc fingers; cysteine rich regions of amino acids are YDR2C PEP7 essential for function YDR2W Hypothetical ORF Karyopherin involved in nuclear import and export; shown to be responsible for nuclear import of replication protein A and for expo several proteins including Swi6p, Far1p, and Phop; cargo dissocia YDR5W MSN5 2 involves binding to RanGTP YDR6W YDR7W MRPS28 Hypothetical ORF Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the small subunit; MRP1 exhibit genetic interactions with PET122, encoding a COX-specific translational activator, and with PET12, encoding a small subunit YDR7W MRP1 mitochondrial ribosomal protein YDR5C TCM1 Mitochondrial inner membrane protein required for assembly of th sector of mitochondrial F1F ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthe

11 Protein of the mitochondrial inner membrane that functions as an dependent chaperone, required for the assembly of the cytochrom bc(1) complex from the Rip1p and Qcr1p proteins; member of th YDR75C BCS1 2 CDC8/PAS1/SEC18 ATPase family Subunit f of the F sector of mitochondrial F1F ATP synthase, wh a large, evolutionarily conserved enzyme complex required for AT YDR77W ATP17 synthesis BAR adaptor protein, subunit of a complex (Rvs161p-Rvs167p) th regulates actin, endocytosis, and viability following starvation or YDR88W RVS167 osmotic stress Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner YDR9W SHE9 5 membrane; forms a homo-oligomeric complex YDR5W YDR55C YDR56W MRP2 NHX1 2 2 Also known as: YmL1 Mitochondrial Ribosomal Protein Hypothetical ORF Required for intracellular sequestration of Na+; Na+/H+ exchange YDR62W MRPL28 Mitochondrial Ribosomal Protein, Large subunit YDR77W SNF1 YDR8W SAC2 5 Protein serine/threonine kinase, required for release from glucose repression, invertase expression, sporulation, and for expression o catabolite-repressed genes when glucose is limiting; regulates Adr dependent transcription primarily at the level of chromatin binding May interact with actin as a component or controller of the assem stability of the actin cytoskeleton; involved in localization of actin chitin YDR9W The authentic, non-tagged protein was localized to the mitochond YDR9W RSM Mitochondrial ribosomal protein of the small subunit YDR95C VPS vacuolar sorting protein; vacuolar protein targeting protein

12 YDR57C GIN 5 Protein kinase involved in bud growth and assembly of the septin proposed to have kinase-dependent and kinase-independent activ undergoes autophosphorylation; similar to Kccp and Hsl1p YDR59W 2 Hypothetical ORF YDR511W ACN9 YDR512C EMI1 2 Non-essential protein of unknown function required for transcriptio induction of the early meiotic-specific transcription factor IME1, al required for sporulation YDR518W EUG1 Protein disulfide isomerase of the endoplasmic reticulum lumen, function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER YDR519W FPR2 Also known as: FKBP1 YDR522C SPS2 Middle/late gene of meiosis; involved in meiosis YDR529C QCR7 2 ubiquinol-cytochrome c oxidoreductase subunit 7 (1 kda) YDR5C HSP1 Possible chaperone and cysteine protease with similarity to E. coli Hsp1 and S. cerevisiae Hsp2p, Hspp, and Snop; member of DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer YEL6W 2 Hypothetical ORF YEL2W RIP1 1 oxidizes ubiquinol at center P in the protonmotive Q cycle mechan transferring one electron to cytochrome c1 and generating a lowpotential ubisemiquinone anion which reduces the low-potential cytochrome b-566 heme group; Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex YEL27W CUP5 5 Proteolipid subunit of the vacuolar H(+)-ATPase V sector (subuni dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeost

13 YEL29C BUD16 Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; similarity to pyridoxal kinases YEL9C CYC7 Cytochrome c isoform 2, expressed under hypoxic conditions; elec carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytoch c oxidase during cellular respiration YEL5C RML2 5 Mitochondrial ribosomal protein of the large subunit, has similarity E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcrip factor YEL51W VMA8 vacuolar ATPase V1 domain subunit D YER1W HEM1 Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyze the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX YER17C AFG Component, with Yta12p, of the mitochondrial inner membrane m protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondria enzyme complexes YER2W YAT2 YER28C MIG YER5C RSM18 5 Carnitine acetyltransferase; has similarity to Yat1p, which is a car acetyltransferase associated with the mitochondrial outer membra Probable transcriptional repressor involved in response to toxic ag such as hydroxyurea that inhibit ribonucleotide reductase; phosphorylation by Snf1p or the Mec1p pathway inactivates Migp allowing induction of damage response genes Mitochondrial ribosomal protein of the small subunit, has similarity E. coli S18 ribosomal protein

14 YER5C PIC2 Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundan than Mir1p under normal conditions; expression is induced at high temperature YER58W PET117 5 Protein required for assembly of cytochrome c oxidase YER61C CEM1 5 homology with beta-keto-acyl synthases; Protein homologous to b keto-acyl synthase YER68W MOT2 Component of the CCR-NOT transcription regulatory complex, wh represses transcription, at least in part, by inhibiting functional TB DNA interactions and also aids in transcription elongation; interact with C-terminal region of Not1p YER69W ARG5,6 Bifunctional enzyme with N-acetyl-gamma-glutamyl-phosphate reductase and acetylglutamate kinase activities, catalyzes the sec and third steps in the biosynthesis of the arginine precursor ornith forms a complex with Arg2p YER7W ALD5 2 5 Mitochondrial aldehyde dehydrogenase that is activated by K+ and utilizes NADP+ as the preferred coenzyme YER77C Hypothetical ORF YER8C RMD7 Required for Meiotic nuclear Division; functions in DNA replication damage response; Protein involved in cell wall function YER86W ILV1 5 Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization i independent of known ILV1 regulation YER87W 2 Hypothetical ORF YER9C-A 2 Hypothetical ORF

15 YER11C KAP12 Karyopherin beta, mediates nuclear import of ribosomal proteins p to assembly into ribosomes and import of histones H and H; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits gen interactions with RAI1 ADP-ribosylation factor GTPase activating protein (ARF GAP), invo YER122C GLO 2 2 in ER-Golgi transport; shares functional similarity with Gcs1p Protein required for the hydroxylation of heme O to form heme A, YER11W COX15 2 which is an essential prosthetic group for cytochrome c oxidase High affinity iron permease involved in the transport of iron across plasma membrane; forms complex with Fetp; expression is regu YER15C FTR1 by iron Specific translational activator for the COX mrna that acts toget with Pet5p and Pet9p; located in the mitochondrial inner YER15C PET membrane Translocase of the mitochondrial inner membrane, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from matrix into the inner membrane, interacts with mitochondrial YER15W OXA1 2 ribosomes; null is respiratory deficient YER17W YER178W ADK2 PDA1 5 Adenylate kinase (mitochondrial GTP:AMP phosphotransferase alpha subunit of pyruvate dehydrogenase (E1 alpha) involved in protection against heat-induced protein aggregation bu necessary for protein import into the mitochondrion; DnaJ homolo YFL16C MDJ1 2 involved in mitochondrial biogenesis and protein folding Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E) of the pyruvate dehydrogenase and 2-oxoglutara YFL18C LPD1 2 2 dehydrogenase multi-enzyme complexes YFL6W RPO1 5 mitochondrial RNA polymerase

16 1-phosphatidylinositol--phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (,5)P2, which is involv YFR19W FAB1 vacuolar sorting and homeostasis YFRC QCR6 ubiquinol-cytochrome c oxidoreductase subunit 6 (17 kda) Basic helix-loop-helix (bhlh) transcription factor; binds cooperativ with Pho2p to the PHO5 promoter; function is regulated by YFRC PHO 2 phosphorylation at multiple sites and by phosphate availability YFR5W 5 Hypothetical ORF Mitochondrial ribosomal protein of the small subunit, has similarity YFR9W YMR1 human mitochondrial ribosomal protein MRP-S6 Phosphatidylinositol -phosphate binding; similar to Fab1 and Vps YGL2C PIB2 involved in telomere-proximal repression of gene expression Probable transcription factor, polyglutamine domain protein; RNA YGL25C PGD1 polymerase II mediator subunit YGL57C YGL6C MRH Hypothetical ORF mitochondrial RNA helicase Transcription factor that binds the consensus site PyPuCACCCPu, involved in iron homeostasis and cell size regulation; activates the YGL71W RCS1 expression of target genes in response to low-iron conditions Multimembrane-spanning protein and putative glycerol transporte YGL8C GUP1 is essential for proton symport of glycerol; Gup2p homolog Protein of the Sec1p family, essential for vacuolar protein sorting; required for the function of both Pep12p and the early endosome/ YGL95C VPS5 5 Golgi SNARE Tlg2p YGL17C RMD9 Mitochondrial protein required for sporulation involved in release from glucose repression, invertase expression, YGL115W SNF sporulation; associates with Snf1p

17 YGL125W MET1 YGL129C RSM2 1 YGL15W RPL1B Isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,1-methylenetetrahydrofolate to 5- methyltetrahydrofolate in the methionine biosynthesis pathway Mitochondrial ribosomal protein of the small subunit, has similarity mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p N-terminally acetylated protein component of the large (6S) ribosomal subunit, nearly identical to Rpl1Bp and has similarity to coli L1 and rat L1a ribosomal proteins; rpl1a rpl1b double null mutation is lethal Mitochondrial rrna Methyltransferase; methylates the 21S YGL16C MRM2 2 (mitochondrial) rrna at position U2791; 2'O-ribose methyltransfe YGL1C MRF1 Mitochondrial polypeptide chain release factor High affinity Ca2+/Mn2+ P-type ATPase, required for the transpor Ca2+ and Mn2+ into the Golgi; involved in Ca2+ dependent proce YGL167C PMR1 such as protein sorting, processing, and degradation Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 9% of ORF overlaps the verified gene MDM; deletion in cyr1 mutant results YGL218W 2 loss of stress resistance Component of the conserved oligomeric Golgi complex; interacts w YGL22C COG1 Cog2p; Interacts with Sec5 YGL26C MTO1 MTO1 - strong similarity to gida E.coli protein

18 YGL27C HAP2 Subunit of the heme-activated, glucose-repressed Hap2p/p/p/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences suffi for both complex assembly and DNA binding Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the A YGL2W DOC1 by contributing to substrate recognition; involved in cyclin proteol YGL25W YGR8C YGR28W STF2 MSP Hypothetical ORF ATPase stabilizing factor kda putative membrane-spanning ATPase Mitochondrial inner membrane protein, required for export of the Cox2p C terminus from the mitochondrial matrix to the intermemb space during its assembly into cytochrome c oxidase; similar to O YGR62C COX of N.crassa YGR76C YGR8C MRPL25 MRP1 also known as: YmL25 Mitochondrial ribosomal protein of the small subunit Tpc1p is a transporter that catalyzes the uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; it is lo in the mitochondrial membrane and its expression appears to be YGR96W TPC regulated by carbon source Mitochondrial serine protease required for the processing of variou mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-glpg superfamily of YGR11W PCP1 1 1 intramembrane peptidases YGR12C 5 Hypothetical ORF

19 YGR15W VMA21 Protein involved in vacuolar H+-ATPase assembly or function; req for the biogenesis of a functional vacuolar ATPase (V-ATPase), but part of the final enzyme complex Mitochondrial inner membrane protein required for normal respira possible chaperone involved in assembly of cytochrome c oxidase; YGR112W SHY similar to SURF1 from mammals, chickens, and D. melanogaster antiproliferative protein involved in determination of replicative life span; mitochondrial protein, prohibitin homolog; similar to S. YGR12C PHB1 cerevisiae Phb2p YGR15C 5 Hypothetical ORF Cystathionine beta-synthase, catalyzes the first committed step of YGR155W CYS transsulfuration in cysteine biosynthesis YGR165W YGR167W YGR171C MRPS5 CLC1 MSM Mitochondrial ribosomal protein of the small subunit Clathrin light chain mitochondrial methionyl-trna synthetase Essential for the expression and activity of ubiquinol-cytochrome c YGR17C CBP 2 reductase; ubiquinol--cytochrome-c reductase assembly factor Poly(A)-binding protein binding protein; interacts with poly(a)-bin YGR178C PBP1 protein Ribonucleotide-diphosphate reductase (RNR), small subunit; the R complex catalyzes the rate-limiting step in dntp synthesis and is regulated by DNA replication and DNA damage checkpoint pathwa YGR18C RNR via localization of the small subunits Translocase of the inner membrane; mitochondrial intermembrane space protein mediating import and insertion of polytopic inner membrane proteins; Subunit of mitochondrial protein import YGR181W TIM1 machinery

20 YGR18C QCR kda subunit 9 of the ubiquinol cytochrome c oxidoreductase complex Plays a structural role in pyruvate dehydrogenase complex; Protei YGR19C PDX1 component of mitochondrial pyruvate dehydrogenase complex YGR27C YGR215W YGR219W YGR22C RSM27 MRPL9 2 Hypothetical ORF Mitochondrial ribosomal protein of the small subunit Hypothetical ORF Also known as: YmL9 Protein required for splicing of the COX1 intron AI5 beta; also specifically required, together with Pet122p and Pet9p, for translation of the COX mrna; located in the mitochondrial inner YGR222W PET5 membrane Protein involved in (1,)-beta-glucan synthesis, possibly through regulation of cell wall glucan and chitin synthesis; chromatin bindi YGR229C SMI1 protein; 57 kda nuclear protein Possible role in aging; mitochondrial protein, prohibitin homolog; YGR21C PHB2 homolog of mammalian BAP7 and S. cerevisiae Phb1p YGR2C PFK1 6-phosphofructokinase beta subunit of succinyl-coa ligase (synthetase; ATP-forming), a mitochondrial enzyme of the TCA cycle; Succinate-CoA Ligase (AD YGR2C LSC2 Forming) Putative flavin-dependent monooxygenase, involved in ubiquinone (Coenzyme Q) biosynthesis; located on the matrix side of the YGR255C COQ6 mitochondrial inner membrane YGR257C YHL5C YHL2C MTM1 OPI1 2 Hypothetical ORF Hypothetical ORF Transcription co-repressor

21 YHL25W SNF6 1 YHL8C CBP2 Involved in global regulation of transcription; subunit of the chrom remodeling Snf/Swi complex Protein required for splicing of COB ai5 intron; Cytochrome B premrna processing protein YHR1W-A QCR1 8.5 kda subunit of the ubiqunol-cytochrome c oxidoreductase com YHR8C YHR9C SOD2 Manganese-containing superoxide dismutase Hypothetical ORF Probable mitochondrial seryl-trna synthetase, mutant displays YHR11W DIA increased invasive and pseudohyphal growth YHR1C ARD1 subunit of the major N alpha-acetyltransferase; complexes with N YHR25W THR homoserine kinase Subunit c'' of the vacuolar ATPase, which functions in acidification YHR26W PPA1 the vacuole; one of three proteolipid subunits of the V domain YHR7W PUT2 2 2 delta-1-pyrroline-5-carboxylate dehydrogenase Ribosomal Recycling Factor 1; originally characterized as FIL1, a F for Isocitrate Lyase expression; mitochondrial ribosome recycling YHR8W RRF1 2 2 factor vacuolar H-ATPase 1 kda subunit of VO membrane sector; vacuo YHR9C-B VMA1 2 ATPase V1 domain subunit G (1 kda) SMF2 was isolated as a high copy suppressor of a temperature sensitive mutation in the PEP ( mitochondrial matrix protease) gen and may influence PEP-dependent protein import; localized to YHR5W SMF2 mitochondrial membrane Subunit VI of cytochrome c oxidase, which is the terminal membe the mitochondrial inner membrane electron transport chain; expre YHR51W COX6 is regulated by oxygen levels

22 YHR67W YHR75C YHR91C YHR1C YHR16W RMD12 PPE1 MSR1 TRR Mitochondrial protein required for sporulation Also known as: YmS2 Nuclear-encoded mitochondrial protein; Arginyl-tRNA synthetase Hypothetical ORF mitochondrial thioredoxin reductase Protein that functions in mitochondrial copper homeostasis and is essential for functional cytochrome oxidase expression; homologo YHR116W COX2 2 COX17, localized to the mitochondrial intermembrane space Translocase of the Outer Mitochondrial membrane, 71.9 kda; 71-k component of the protein translocase of the outer membrane of YHR117W TOM71 mitochondria YHR12W YHR17C MSH1 MRPL6 2 muts homolog involved in mitochondrial DNA repair Also known as: YmL16 Mitochondrial GTP binding protein with similarity to members of th Obg family; associates with the large mitochondrial ribosomal sub and is required for mitochondrial translation; partially suppresses YHR168W 2 petite formation in a mrm2 mutant YHR179W OYE2 1 2 Also known as: Old Yellow Enzyme peptidyl-trna hydrolase; peptidyl-trna hydrolase dispensable for YHR189W PTH1 1 2 growth and for mitochondrial respiration Pvruvate transporter of the mitochondrial inner membrane, memb the mitochondrial carrier family; has putative mouse and human YIL6W 2 orthologs Basic leucine zipper (bzip) transcription factor of the ATF/CREB fa activates transcription of genes involved in utilization of non-optim YIL6W CST6 carbon sources YILC CBR1 cytochrome b reductase

23 -kda mitochondrial acidic matrix protein; Mitochondrial protein YIL7C MAM 1 involved in respiration YIL9C RSM25 5 Mitochondrial ribosomal protein of the small subunit YIL9C LYS12 5 Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enz required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Mitochondrial matrix protein, required for assembly or stability at YIL98C FMC1 5 temperature of the F1 sector of mitochondrial F1F ATP synthase Subunit Vb of cytochrome c oxidase, which is the terminal membe the mitochondrial inner membrane electron transport chain; predominantly expressed during anaerobic growth while its isoform YIL111W COX5B (Cox5Ap) is expressed during aerobic growth Putative mitochondrial porin (voltage-dependent anion channel), related to Por1p but not required for mitochondrial membrane YIL11C POR2 2 permeability or mitochondrial osmotic stability YIL125W KGD1 alpha-ketoglutarate dehydrogenase DNA repair and TFIIH regulator, required for both nucleotide excis repair (NER) and RNA polymerase II (RNAP II) transcription; poss role in assembly of a multiprotein complex(es) required for NER a YIL128W MET18 RNAP II transcription YIL1W FLX1 2 2 YIL16W OM5 5 YIL15C IMP2' 5 Protein required for transport of flavin adenine dinucleotide (FAD) mitochondria, where it is synthesized from riboflavin, to the cytos Protein of unknown function, major constituent of the mitochondri outer membrane; located on the outer (cytosolic) face of the oute membrane

24 YIL155C GUT2 Mitochondrial glycerol--phosphate dehydrogenase; expression is repressed by both glucose and camp and derepressed by nonfermentable carbon sources in a Snf1p, Rsf1p, Hap2///5 comple dependent manner YIL157C The authentic, non-tagged protein was localized to the mitochond U2B component of U2 snrnp, involved in splicing, binds the U2 sn stem-loop IV in vitro; does not contain the conserved C-terminal R YIR9W MSL1 5 binding domain found in other family members Protein required for the splicing of two mitochondrial group I intro (BI in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI-enc YIR21W MRS1 2 5 maturase, that binds to the BI RNA YIR2C YJLW YJLC YJL22W YJL2C GIF1 COX16 SYS1 PET (putative) involved in cell cycle control Multicopy suppressor of ypt6 null mutation Hypothetical ORF Protein required for respiratory growth Required for Vacuolar Protein Sorting; Vps5p is a hydrophilic pro that is peripherally associated with the late Golgi and forms a stab YJL29C VPS5 2 complex with Vps52p and Vps5p. YJL5W YJL6W YJL6C MRPL8 2 Similar to SDH1 Hypothetical ORF Also known as: YmL8 Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthe YJL71W ARG2 of the arginine precursor ornithine; forms a complex with Arg5,6p YJL9C KHA1 2 putative K+/H+ antiporter

25 YJL96W MRPL9 Also known as: YmL9 YJL11C GSH1 2 5 glutamate-cysteine ligase YJL12W MEF2 2 mitochondrial elongation factor G-like protein YJL117W PHO86 5 Protein specifically required for packaging of the high-affinity phosphate transporter Pho8p into COPII coated vesicles for trans to the plasma membrane; transcription and localization are regula by phosphate levels YJL121C RPE1 D-ribulose-5-Phosphate -epimerase YJL1W MRS 2 Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similar to and functionally redundant with Mrsp; fun under low-iron conditions; may transport other cations in addition iron YJL155C FBP26 2 Also known as: FBPase-2 YJL166W QCR8 Ubiquinol cytochrome-c reductase subunit 8 (11 kda protein) YJL175W 2 Hypothetical ORF YJL176C SWI 2 transcription factor YJL18C ATP12 2 Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F ATP synthase YJL19W 2 2 Hypothetical ORF YJL2C 1 1 Hypothetical ORF YJL28C NUC1 2 Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has a role in mitochondrial recombination YJL29W CBP1 2 Protein required for COB mrna stability or 5' processing, required translation of COB mrnas YJR18W 2 Hypothetical ORF YJRC RAV1 Regulator of (H+)-ATPase in vacuolar membrane YJRW PET191 Protein required for assembly of cytochrome c oxidase

26 YJRW GEF1 5 Chloride channel localized to late- or post-golgi vesicles, involved iron metabolism; highly homologous to voltage-gated chloride channels in vertebrates YJR8W CYC1 1 Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinonecytochrome c oxidoreductase to cytochrome c oxidase during cellu respiration YJR51W OSM1 5 5 osmotic growth protein YJR7W MOG1 5 Conserved nuclear protein that interacts with GTP-Gsp1p, which is Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p YJR77C MIR Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abunda than Pic2 under normal conditions YJR79W 5 Hypothetical ORF YJR95W SFC1 2 Mitochondrial succinate-fumarate transporter, transports succinate and fumarate out of the mitochondrion; required for ethanol and acetate utilization YJR12C VPS25 2 vacuolar protein sorting (putative) YJR11C RSM Mitochondrial ribosomal protein of the small subunit, has similarity E. coli S7 ribosomal protein YJR12W 2 Hypothetical ORF YJR121W ATP2 1 Beta subunit of the F1 sector of mitochondrial F1F ATP synthase, which is a large, evolutionarily conserved enzyme complex require ATP synthesis YJR122W CAF17 CCR associated factor; component of CCR transcriptional compl

27 Involved in mitochondrial genome maintenance; (putative) nucleic YJR1W MGM11 interactor YKL2W DID 5 Mitochondrial ribosomal protein of the small subunit; MRP17 exhib genetic interactions with PET122, encoding a COX-specific YKLC MRP17 translational activator YKL11C CCE1 cruciform cutting endonuclease Subunit d of the stator stalk of mitochondrial F1F ATP synthase, which is a large, evolutionarily conserved enzyme complex require YKL16C ATP7 5 ATP synthesis YKL29C YKL7W YKLC YKL1W MAE1 NFU1 VPS2 5 5 Mitochondrial malic enzyme; mitochondrial malic enzyme Hypothetical ORF Nifu-like protein involved in secretion Mitochondrial Distribution and Morphology; Similar to human sequ YKL5C-A MDM5 2 2 predicted by GENSCAN Mitochondrial -oxoacyl-[acyl-carrier-protein] reductase, may com YKL55C OAR a type II mitochondrial fatty acid synthase along with Mct1p 2 kda subunit of V1 sector; vacuolar ATPase V1 domain subunit YKL8W VMA kda) YKL85W MDH mitochondrial malate dehydrogenase Links heme covalently to apocytochrome c1; cytochrome c1 heme YKL87C CYT lyase YKL9W MBR1 Involved in mitochondrial biogenesis; MBR1 protein precursor YKL16W AAT1 2 2 Mitochondrial aspartate aminotransferase, catalyzes the conversio oxaloacetate to aspartate in aspartate and asparagine biosynthesi

28 Subunit of the heme-activated, glucose-repressed Hap2p/p/p/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal YKL19W HAP activation function of the complex YKL118W 1 2 Involved in meiotic nuclear division. Protein involved in vacuolar H+-ATPase assembly or function; req for the biogenesis of a functional vacuolar ATPase (V-ATPase), but YKL119C VPH2 2 2 part of the final enzyme complex Mitochondrial inner membrane transporter, transports oxaloacetat YKL12W OAC1 5 sulfate, and thiosulfate; member of the mitochondrial carrier fami Mitochondrial intermediate peptidase, cleaves N-terminal residues subset of proteins upon import, after their cleavage by mitochond processing peptidase (Mas1p-Mas2p); may contribute to mitochon YKL1C OCT1. iron homeostasis YKL17W YKL18C MRPL Hypothetical ORF Also known as: YmL1 Putative mitochondrial ribosomal protein, has similarity to E. coli YKL12W MRP8 ribosomal protein S2 Flavoprotein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdhp, Sdhp), which couples the oxidation of succinate to the YKL18C SDH transfer of electrons to ubiquinone YKL15W YKL155C MCR1 RSM22 2 NADH-cytochrome b5 reductase Mitochondrial ribosomal protein of the small subunit Mitochondrial ribosomal protein of the large subunit, not essential YKL167C MRP mitochondrial translation YKL169C YKL17W YKL19C MRPL8 MST1 1 Hypothetical ORF Also known as: YmL8, YmL mitochondrial threonine-trna synthetase

29 Subunit of complex involved in processing of the ' end of cytochr YKL28W CBT1 2 2 b pre-mrna; involved in RNA splicing Involved in vacuolar protein sorting and normal organization of intracellular membranes; probably required for membrane-protein retention in a late Golgi compartment; putative GTP-binding prote YKR1C VPS1 2 similar to mammalian Mx proteins YKR6C MRPL1 2 Also known as: YmL1 YKR16W 2 2 The authentic, non-tagged protein was localized to the mitochond YKR2W UTH1 2 5 Youth, involved in determining yeast longevity; involved in cell gro Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similar to and functionally redundant with Mrsp; fun under low-iron conditions; may transport other cations in addition YKR52C MRS iron YKR66C YKR85C CCP1 MRPL2 Cytochrome-c peroxidase Also known as: YmL2 Dynamin-related GTPase required for mitochondrial fission and the maintenance of mitochondrial morphology, assembles on the cytoplasmic face of mitochondrial tubules at sites at which division YLL1W DNM1 occur; also participates in endocytosis Involved in mechanism by which mitochondrial shape is establishe YLL6W MMM1 maintained; mitochondrial outer membrane protein Copper metallochaperone that shuttles copper from the cytosol to mitochondrial intermembrane space for delivery to cytochrome c YLL9C COX17 oxidase

30 Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper YLL18C-A COX19 metallochaperone that delivers copper to cytochrome c oxidase YLL2C YLL27W YLLW SSA2 ISA1 GON2 member of 7 kda heat shock protein family ISA1 - mitochondrial protein required for normal iron metabolism Uncharacterized Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sd Sdhp, Sdhp), which couples the oxidation of succinate to the YLL1C SDH2 transfer of electrons to ubiquinone Involved in derepression of SUC2 in response to glucose limitation YLR25W SNF7 2 involved in glucose derepression YLR27C AAT2 Putative metal transporter, Nramp homolog, homolog of SMF1 and YLRC SMF SMF2 Subunit VIb of cytochrome c oxidase, which is the terminal memb the mitochondrial inner membrane electron transport chain; requi for assembly of fully active cytochrome c oxidase but not required YLR8C COX activity after assembly C-5 sterol desaturase, catalyzes the introduction of a C-5(6) doub bond into episterol, a precursor in ergosterol biosynthesis; mutant YLR56W ERG 2 viable, but cannot grow on non-fermentable carbon sources YLR59C REX2 RNA exonuclease Specific translational activator for the COX1 mrna, also influences stability of intron-containing COX1 primary transcripts; located in YLR67C PET9 mitochondrial inner membrane YLR69C MEF1 mitochondrial elongation factor G-like protein

31 YLR77W The authentic, non-tagged protein was localized to the mitochond Protein whose 2kDa cleavage product is found in endosome-enric YLR8C EMP7 membrane fractions, predicted to be a transmembrane protein YLR87C YLR91W YLR19W CSF1 AHP1 2 Protein required for fermentation at low temperature Hypothetical ORF AHP1 - Alkyl hydroperoxide reductase Protein involved in mitochondrial gene expression, may facilitate translation by delivering mrnas to membrane-bound translationa YLR19C SLS1 activation complexes; 7 kda mitochondrial integral membrane pr YLR12W YLR18W YLR19C YLR168C PUT1 PEP MSF proline oxidase vacuolar membrane protein Hypothetical ORF possibly involved in intramitochondrial sorting Transcription cofactor, forms complexes with DNA-binding protein Swip and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulated by YLR182W SWI6 phosphorylation; potential Cdc28p substrate YLR19C 2 Hypothetical ORF YLR21C The authentic, non-tagged protein was localized to the mitochond YLR22C Hypothetical ORF Protein required for the maturation and translation of COX1 mrna YLR2C MSS51 involved in maturation of COX1 and COB mrna YLR2W YLR218C YLR22W QRI5 2 2 Mitochondrial protein of unknown function Hypothetical ORF Hypothetical ORF

32 YLR25C Involved in meiotic nuclear division. Lipoyl ligase, involved in the modification of mitochondrial enzyme YLR29C LIP2 the attachment of lipoic acid groups Minor sphingoid long-chain base kinase, paralog of Lcbp respons for few percent of the total activity, possibly involved in synthesis YLR26W LCB5 2 long-chain base phosphates, which function as signaling molecule Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Gol YLR262C YPT6 has similarity to the human GTPase, Rab6 Identified in a screen for dense cells that accumulated invertase a non-permissive temperature, SEC22 encodes a v-snare present o to Golgi vesicles and is involved in anterograde and retrograde transport between the ER and Golgi; Synaptobrevin (v-snare) YLR268W SEC22 homolog Non-essential hydrolase involved in mrna decapping, may functio a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interac YLR27W DCS with neutral trehalase Nth1p YLR29C 2 Hypothetical ORF Subunit h of the F sector of mitochondrial F1F ATP synthase, wh is a large, evolutionarily conserved enzyme complex required for A YLR295C ATP1 synthesis Mitochondrial aconitase, required for the tricarboxylic acid (TCA) c YLRC ACO1 mutation leads to glutamate auxotrophy YLR12W-A MRPL15 YLR8W 2 2 Also known as: YmL15 Hypothetical ORF

33 Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates in YLR8C DIC the mitochondrial matrix YLR68W MDM 2 Protein involved in determination of mitochondrial structure Mitochondrial hsp7-type molecular chaperone, involved in the YLR69W SSQ1 synthesis and assembly of iron/sulfur clusters into proteins YLR77C YLR82C FBP1 NAM2 Also known as: FBPase Mitochondrial leucyl trna synthetase Mitochondrial inner membrane protein required for assembly of th sector of mitochondrial F1F ATP synthase, interacts genetically w YLR9W ATP1 2 2 ATP6 YLR95C COX8 YLR96C VPS 2 2 YLR17W VPS6 Subunit VIII of cytochrome c oxidase, which is the terminal memb the mitochondrial inner membrane electron transport chain Vacuolar sorting protein essential for vacuolar morphogenesis and function; involved in vacuolar protein targeting Defective in vacuolar protein sorting; involved in vacuolar protein targeting YLR2C APG17 Required for activation of Apg1 protein kinase; involved in autoph Guanine nucleotide exchange factor (GEF) that functions to modu Rhop1 activity as part of the cell integrity signaling pathway; mult suppressor of tor2 mutation and ypk1 ypk2 double mutation; pote YLR25W TUS1 Cdc28p substrate YLR9W YLR7C YML9C YML21C MRPL VMA6 MRPL9 UNG Also known as: YmL vacuolar ATPase V domain subunit d (6 kda) Also known as: YmL9 Uracil DNA glycosylase

34 YMLW 2 2 Hypothetical ORF YML2W CAT2 2 2 Carnitine O-acetyltransferase, peroxisomal and mitochondrial Expression is repressed by glucose and anaerobic conditions, is induced by L-lactate and is regulated by GRR1, ROX, HAP1, HXK YML5C CYB2 2 CYC8; Cytochrome b2 [L--lactate cytochrome-c oxidoreductase] Involved in repair and recombination of mitochondrial DNA; also p a role in (nuclear) chromosomal telomere formation and elongatio YML61C PIF1 2 to ' DNA helicase Protein involved in mitochondrial import of fusion proteins; YML62C MFT1 mitochondrial targeting protein Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal proline residues; involved in protein refolding after import into YML78W CPR 2 2 mitochondria Subunit of the mitochondrial F1F ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in YML81C-A ATP18 2 other organisms YML89C YML9W Hypothetical ORF Hypothetical ORF 2-hexaprenyl-6-methoxy-1,-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; located in YML11C COQ5 mitochondria NADH:ubiquinone oxidoreductase, transfers electrons from NADH ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex YML12C NDI1 which is absent in S. cerevisiae

35 YML129C COX1 Mitochondrial membrane protein, required for assembly of cytochr c oxidase YMR12W CLU1 2 CLU1 - translation initiation factor eif (p15 subunit) YMR21C MAC1 Metal-binding transcriptional activator YMR2C MSS May play a part in mitochondrial translation; putative mitochondri GTPase YMR2W MRPL 2 Also known as: YmL YMR1W-A Hypothetical ORF YMR5W IMP2 Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p YMR5W STV1 2 Stv1p and Vph1p may be equivalent subunits for vacuolar-type H( ATPases located on different organelles; vacuolar ATPase V doma subunit a (11 kda) YMR56C AAC1 Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; Aac1p is a min isoform while Pet9p is the major ADP/ATP translocator YMR6C TOM7 Component of the mitochondrial outer membrane sorting and asse machinery (SAM) complex; required for the sorting of some prote the outer membrane after import by the TOM complex; mitochond import receptor, heterodimerizes with Tom7p, preferentially recognizes the mature regions of precursor proteins associated wi ATP-dependent cytosolic chaperones YMR62C ECM Mitochondrial ornithine acetyltransferase, catalyzes the fifth step i arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine YMR6W RIM9 Protein required for IME1 expression; involved in sporulation