Shim-pack HPLC Column Guidebook

Transcription

1 High Performance Liquid Chromatograph s Shimpack HPLC Guidebook C90E6

2 Shimadzu Liquid Chromatographs Maximizing the Potential of UHPLC/HPLC Analysis Meet the Nexera X, the most advanced UHPLC available today. The flexible system design achieves a true fusion between UHPLC and HPLC technologies, enabling the Nexera X to be used for a much broader range of applications. In addition to providing maximum speed, sensitivity, resolution, stability, and reliability, it also features the revolutionary ipdea* separation technology and an idrec** function that extends the dynamic range so that both concentrated and trace components can be quantitated simultaneously. * Intelligent Peak Deconvolution Analysis (patent pending) ** Intelligent Dynamic Range Extension Calculator (patent pending) The Most Accessible UHPLC Available Developed with expandability and compatibility in mind, the Nexera XR ultra high performance liquid chromatograph enables more customers to make use of highspeed, highresolution systems. Configure the optimal system to meet the specific analysis objective by selecting from among a wide range of highly accurate and reliable modules. The next milestone in the evolution of liquid chromatography, the Nexera XR promises to become an indispensable tool in laboratories in a variety of fields, including pharmaceuticals, biochemistry, chemistry, environmental, and foods. Selection Guide P. 4 UHPLC/HPLC s P. Shimpack XR Series P. Shimpack VP Series P. 4 Shimpack MAqCODS P. 8 Contents Shimpack G Series P. Shimpack FCODS P. SFC s P. 7 Shimpack UCX Series P. 7

3 New Industry Standard HPLCs innovative Realization of Advanced Laboratory Advanced functions free operators from the laboratory Remote monitoring using a smart device Excellent baseline stability unaffected by circumstances Ultrafast injection cycle reduces analysis times Ultralow carryover Use of multiple detectors expands application range intuitive Achieving Easier Operation Unified graphical user interface between system and workstation Simplified batch file creation Displays chromatograms in real time intelligent Smart Features Increase Work Efficiency Fully automated analysis: reliably perform analyses according to prescribed procedures Migrate existing methods from either Shimadzu or nonshimadzu systems HPLC systems are currently used in a wide variety of fields. Reliable analytical data and a more efficient total analysis workflow are required to achieve faster development of new drugs, ensure food safety, and meet higher standards in environmental regulations. Many analysis techniques use LCMS, which requires a frontend HPLC system to provide solvent delivery performance in the micro to semimicro range and injectors with low sample carryover. Prominence is a networkready HPLC system that meets the demands of today s advanced users. Prominence features the world s first Web control, fastest sample injection, and highest detection sensitivity performance functions that surpass current HPLC technology. Size Exclusion s P. 40 Shimpack GPC Series P. 40 Shimpack Diol Series P. 4 Ion Chromatography s P. 4 Shimpack IC Series P. 4 Dedicated s P. 47 Pretreatment s P. Preparative s P. Shimpack PREP Series P. Fittings P. 7 Mobile Phase Cleaner for UHPLC/HPLC P. 8 Ghost Trap DS/DSHP P. 8 LC/MS Certified Vials P. 9 LabTotal Vial P. 9 Shimpack MAYI Series P. Shimpack SPC Series P. 4

4 Selection Guide Selection by Support Materials Packing Materials Silica Gel Type Polymer Type Shimpack XR, VP, MAqCODS, G Series, FCODS Shimpack UCX Series Shimpack Diol Series Shimpack WAX/WCX Series Shimpack GPC Series Shimpack IC Series Shimpack Amino, SCR, ISA/ISC, SPR Series Selection by Separation Modes Reversed Phase ODS/C8 (Shimpack XR, VP, MAqC, G, FC Series) C8 (Shimpack XR, VP, G Series) Phenyl (Shimpack XR, VP, G Series) Low molecularweight compounds Silica Gel Type Normal Phase Shimpack GIS CN Steroid hormones Shimpack XRSIL Phospholipids Shimpack GIST NH Saccharides, tocopherols Ion Exchange Shimpack WAX (weakly basic anion exchange) Nucleotides, proteins Shimpack WCX (weakly acidic cation exchange) Proteins Gel Filtration Shimpack Diol Series Proteins Ion Exchange Shimpack IC Series Inorganic ions Shimpack Amino, ISC Series Amino acids, guanidines Shimpack ISA Series Saccharides Polymer Type Ion Exclusion Shimpack SCRH, SCRH Shimpack SPRH Organic acids Size Exclusion Shimpack SPRNa Trisaccharides or below, organic acids GPC Shimpack GPC Series Synthetic high polymers 4

5 Selection by s Analytical s HighSpeed Analysis.6 µm,. µm.9 µm, µm,. µm, µm Shimpack XRODS III Shimpack G, XR Series HPLC s Conventional Analysis µm Shimpack VP, MAqCODS, G Series, FCODS Preparative s µm, µm µm Shimpack G Series Shimpack PREP Series Selection of ODS columns With Certificate of Compliance for Validation Shimpack XRODS (Pressure tolerance: MPa) P. Shimpack XRODS II (Pressure tolerance: 60MPa) P. Shimpack XRODS III (Pressure tolerance: 0MPa) P. HighSpeed Analysis Wider ph Range Rapid Separation and Wider ph Range Shimpack GIST/GISTHP C8 (ph range: ) P. Shimpack GISS/GISSHP C8 (ph range: ) P. Stronger Retentivity Shimpack GIS/GISHP C8 P. 4 With Certificate of Compliance for Validation Shimpack VPODS P. 4 Wider ph Range Shimpack GIST C8 (ph range: ) P. Conventional Analysis Stronger Retentivity Shimpack GIS C8 P. 4 Shimpack FCODS (ph range:. 9) P. Higher CostPerformance Shimpack GWS C8 P. 4 Strong Retentivity of Basic Compounds Shimpack MAqCODS P. 8 Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

6 Selection Guide Separation Mode Selection by Target Compounds Insoluble in Ethanol High Polar Acid Compounds High Polar Neutral Compounds Anion Exchange Ion Exclusion Normal Phase High Polar Basic Compounds Cation Exchange Soluble in Water Soluble in Ethanol MidPolar Neutral Compounds Insoluble in Acid Water Low Polar Acid Salts Target Compounds Insoluble in Basic Water Soluble in Acid Water Low Polar Basic Salts Low Polar Basic Compounds Reversed Phase Insoluble in Water Soluble in Basic Water Soluble in Ethanol Low Polar Acid Compounds MidPolar Neutral Compounds Normal Phase Insoluble in Ethanol Polymer Compounds Size Exclusion Selection by Target Compounds Saturation Aliphatic Carboxylic Acids Carboxylic Acids Aliphatic Carboxylic Acids Nonsaturation Aliphatic Carboxylic Acids Hydroxy and Keto Carboxylic Acids Reversed Phase Ion Exclusion Anion Exchange Shimpack GIST C8 P. Shimpack SCRH P. 48 Shimpack SCRH P. 48 Shimpack WAX P. Shimpack ICA P. 4 Aromatic Carboxylic Acids Reversed Phase Shimpack GIST C8 P. 6

7 Selection by Target Compounds Free Amino Acids Cation Exchange Shimpack AMINOLI P. 47 (Postcolumn derivation fluorescing with OPA) Amino Acids Cation Exchange Shimpack AMINONA P. 47 (Postcolumn derivation fluorescing with OPA) Proteinhydrolyzed Amino Acids Reversed Phase Reversed Phase + Ion Pair Shimpack GIST C8 P. (Precolumn derivatization) Shimpack GIST C8 P. Peptides Reversed Phase Shimpack GIST C8 P. Peptides Proteins Gel Filtration Shimpack Diol Series P. 4 Proteins Cation Exchange Shimpack WCX P. Anion Exchange Shimpack WAX P. HILIC Mode Shimpack GIST NH P. 0 Monosaccharides Gel Filtration + Ligand Exchange Shimpack SCRN/C/P P. 48 Anion Exchange Shimpack ISA07 P. 49 Sugars Mono to trisaccharides Size Exclusion Size Exclusion + Ligand Exchange Shimpack SPRNa P. 0 Shimpack SPRNa P. 0 Oligosaccharides HILIC Mode Shimpack GIST NH P. 0 Sugar Alcohols Size Exclusion + Ligand Exchange Shimpack SPRPb P. 0 Derivatized Sugars Reversed Phase Shimpack GIST C8 P. Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 7

8 Selection Guide Selection by Target Compounds Mixture of Watersoluble / Fatsoluble vitamins Reversed Phase Shimpack GIST C8 P. Vitamins Watersoluble Vitamins Vitamin C Others HILIC Mode Reversed Phase Shimpack GIST NH P. 0 Shimpack MAqCODS P. 8 Fatsoluble Vitamins Vitamin A, K Vitamin D, E Reversed Phase Normal Phase Shimpack GIST C8 P. Shimpack GIST NH P. 0 Watersoluble Vitamins Vitamin B (thiamin) UV (70 nm) RF (Postcolumn derivatization) Vitamin A (retinol) UV (70 nm) Vitamin D Vitamin B (riboflavin) RF (Postcolumn derivatization) Vitamin E (tocopherol) Vitamin B (niacin) UV (60 nm) Vitamin B (pantothenic acid) UV ( nm) Vitamin B6 (pyridoxine) UV (90 nm) Vitamin K Vitamin B (cyanocobalamine) Vitamin BT (carnitine) Vitamin C (ascorbic acid) Vitamin H (biotin) Vitamin M (folic acid) Vitamin P (hesperidin) UV (80 nm) UV ( nm) RI UV (4 nm) UV ( nm) UV (80 nm) UV (6 nm) * The above wavelength values are approximate values. It is possible to be influenced by the state of mobile phase. Fatsoluble Vitamins UV ( nm) RF (Ex. 40 nm, Em. 460 nm) UV (6 nm) UV (9 nm) RF (Ex. 9 nm, Em. nm) UV ( nm) RF (Postcolumn derivatization) Nucleic Acid Bases Nucleosides Cation Exchange Reversed Phase Shimpack WCX P. Shimpack GIST C8 P. Nucleic Acids Nucleotides Anion Exchange Reversed Phase Shimpack WAX P. Shimpack GIST C8 P. DNA and RNA Gel Filtration Anion Exchange Shimpack Diol Series P. 4 Shimpack WAX P. 8

9 Selection by USP USP Code Packing Shimpack HPLC s Page Shimpack XRODS Shimpack XRODS II Shimpack XRODS III Shimpack VPODS Shimpack GIST C8 4 L Shimpack GISTHP C8 Octadecyl silane chemically bonded to porous or nonporous silica or ceramic microparticles,. to Shimpack GISS C8 µm in diameter, or a monolithic rod. Shimpack GISSHP C8 Shimpack GIS C8 Shimpack GISHP C8 Shimpack GWS C8 Shimpack FCODS Shimpack UCX GIS II L Porous silica particles,. to µm in diameter, or a monolithic silica rod. Shimpack XRSIL Shimpack UCX Sil 7 Shimpack XRC8 L7 Octylsilane chemically bonded to totally or superficially porous silica particles,. to µm in Shimpack VPC8 4 diameter, or a monolithic silica rod. Shimpack GIST C8 6 Shimpack GISTHP C8 6 L8 L An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica Nitrile groups chemically bonded to porous silica particles,. to µm in diameter, or a monolithic Shimpack GIST NH Shimpack GIS CN 0 gel support,. to µm in diameter, or a monolithic silica rod. silica rod. Shimpack UCX NH Shimpack UCX CN 7 7 Shimpack XRPhenyl L Shimpack VPPhenyl Phenyl groups chemically bonded to porous silica particles,. to µm in diameter, or a monolithic 4 Shimpack GIST Phenyl silica rod. 8 Shimpack UCX Phenyl Shimpack GISTHP Phenyl 7 8 Strong cationexchange resin consisting of sulfonated crosslinked styrenedivinylbenzene copolymer L7 in the hydrogen form, 6 to µm in diameter. Strong cationexchange resin consisting of sulfonated crosslinked styrenedivinylbenzene copolymer L9 in the calcium form, µm in diameter. Dihydroxypropane groups chemically bonded to porous silica or hybrid particles,. to µm in Shimpack SCRH Shimpack SCRC L L diameter, or a monolithic silica rod. A rigid, spherical styrenedivinylbenzene copolymer, to 0 µm in diameter. Shimpack UCX Diol Shimpack GPC Series Shimpack ICC L A cationexchange resin made of porous polystyrene gel with sulfonic acid groups, µm in Shimpack AMINOLI 47 diameter. Shimpack AMINONA 47 Shimpack ISC 49 L L8 An anionexchange resin made of porous polymethacrylate or polyacrylate gel with quartenary ammonium groups, 7 µm in size. Strong cationexchange resin consisting of sulfonated crosslinked styrenedivinylbenzene copolymer in the sodium form, about 6 to 0 µm diameter. Packing for the sizeexclusion separations of proteins (separation by molecular weight) over the range Shimpack ICA Shimpack SCRN 4 48 L9 of to 7000 kda. The packing is spherical. µm, silica or hybrid packing with a hydrophilic Shimpack Diol0 4 coating. Shimpack Diol00 4 Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 9

10 UHPLC/HPLC s Shimpack XR Series Shimpack XR Series s Offer Versatility and Fast Analysis Shimpack XR Series columns use a. µm packing particle size and offer a skillful balance between resolution efficiency and pressure. An XR Series column provides resolution equivalent to a generalpurpose column with µm packing particle size (Shimpack VPODS), but significantly reduces the analysis time. The pressure on the column under many analysis conditions does not exceed MPa. Consequently, ultrafast analysis can be comfortably performed on an existing instrument. Pore Size (nm) Surface Area (m/g) Carbon Loading Pressure Tolerance (MPa) Pore Volume (ml/g) Endcapping Bonding Type ph Range USP Code Shimpack XRODS Shimpack XRC8 Shimpack XRPhenyl Shimpack XRSIL % % % Yes Yes Yes Monomeric Monomeric Monomeric L L7 L L Shimpack XRODS Permits Simple Switching from Conventional Analysis The two chromatograms to the right show differences in analysis times when using different columns. The lower chromatogram is the result of analysis using a Shimadzu Shimpack VPODS generalpurpose column. The upper chromatogram is from analysis with a Shimpack XRODS fast analysis column. As both Shimpack VPODS and Shimpack XRODS offer identical resolution properties, Shimpack XRODS maintains the resolution while significantly reducing analysis times. For more information of smooth transfer of methods from highspeed analysis to conventional analysis, please refer to page 6. Shimpack XR Series Comprehensive Product Range In addition to the versatile Shimpack XRODS that is bonded with the C8 group, the comprehensive Shimpack XR Series product range includes the Shimpack XRC8 that is bonded with the C8 group to give different retention behavior to ODS, Shimpack XRPhenyl that is bonded with the phenylpropyl group, and the normalphase Shimpack XRSIL silica column that achieves higher speeds.

11 Analysis Examples Analysis of FatSoluble Vitamins The fatsoluble vitamins E (tocopherol) and A (retinol) were analyzed. The Shimpack XRC8 column achieves higher speed than a conventional C8 column. Peaks. αtocopherol. Retinol Shimpack CLCC8 4.6 mm i.d. 0 mm Shimpack XRODS.0 mm i.d. 7 mm Shimpack XRC8.0 mm i.d. 7 mm min Mobile Phase Flow Rate Col. Temp. Detection : Shimpack XRC8 (7 mml..0 mmi.d.,. µm) (P/N: 89909) : Methanol :.0 ml/min : 40 C : UV 90 nm Analysis of NonSteroidal AntiInflammatory Drug The column was switched from Shimpack XRODS to Shimpack XRPhenyl to improve the resolution. The difference in retention properties between the ODS group and the phenylpropyl group improves the peak shape, controls the resolution, and reduces the analysis time. Peaks. Piroxicam. Ketoprofen. Diflunisal Diclofenac. Indomethacin 6. Ibuprofen 4 Shimpack XRODS.0 mm i.d. 7 mm Shimpack XRPhenyl.0 mm i.d. 7 mm min 6 Mobile Phase Flow Rate Col. Temp. Detection : Shimpack XRPhenyl (7 mml..0 mmi.d.,. µm) (P/N: ) : A) mmol/l Phosphate buffer solution (ph.) B) Acetonitrile A/B = 0/ (v/v) :.0 ml/min : 40 C : UV nm Fast NormalPhase Analysis Organic solvents are used as the mobile phase for normalphase analysis. Due to environmental considerations, it is necessary to reduce the consumption of mobile phase compared with normal reversedphase analysis. The Shimpack XRSIL silica column increases the speed of normalphase analysis while reducing the consumption of mobile phase. In this example, the analysis time is reduced by 80 % while maintaining the flow rate, thereby reducing the overall mobile phase consumption to % or less. Peaks. pbromoanilide Shimpack CLCSIL 4.6 mm i.d. 0 mm Shimpack XRSIL.0 mm i.d. 7 mm min Mobile Phase Flow Rate Col. Temp. Detection : Shimpack XRSIL (7 mml..0 mmi.d.,. µm) (P/N: ) : Hexane / Ethanol = 90/ (v/v) :.0 ml/min : 40 C : UV 4 nm Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

12 UHPLC/HPLC s Shimpack XR Series Shimpack XR Series, Shimpack XRODSII and XRODS III HighPressure s for Higher Resolution and Sensitivity While the Shimpack XRODSII and XRODS III use the same. µm packing particle size as the Shimpack XR Series columns, they have higher 60 and 0MPa pressure tolerance. This allows them to achieve highresolution fast analysis in a long column using a water/methanol mobile phase. This column significantly extends the range of applications of highresolution fast analysis to include analysis near room temperature. The Shimpack XRODSII and XRODS III columns are ideal for Length (mm) (μm) Pore Size (nm) Surface Area (m/g) Carbon Loading Pressure Tolerance (MPa) Pore Volume (ml/g) Endcapping Shimpack XRODS II 0, 0, 7, 0, % 60 Yes Shimpack XRODS III 0, 7 0, % % Yes Yes the Nexera UHPLC or Prominence UFLC. This combination Bonding Type Monomeric Monomeric Monomeric achieves both faster speed and higher resolution. ph Range USP Code 7. L 7. L 7. L Extensive product range, including. mmi.d. column to reduce mobile phase consumption The Shimpack XRODS II range includes a. mmi.d. model in addition to normal mm and mmi.d. columns. With an optimal flow rate of 0. to 0. ml/min, the. mmi.d. column offers the optimal flow rate for LC/MS and reduces mobile phase consumption. Select a column to suit your purpose, whether shorter analysis times or high resolution The Shimpack XRODS III lineup features two columns: a short one utilizing a packing material with a particle size of.6 µm and a long one utilizing a. µm particle size, which is equivalent to the conventional XR column. This extensive lineup allows users to select a column according to analysis objectives, whether it's a short size to further shorten analysis times, or a long size to achieve high resolution while retaining the ease of use of the conventional XR column. Peaks. Acetophenone. Propiophenone. Butyrophenone 4. Valerophenone. Hexanophenone Shimpack VPODS (0 mml. 4.6 mmi.d., µm, P/N: 84979) NTP of Peak =,98 RT of Peak = 4.79 min 4 Mobile Phase Flow Rate Col. Temp. Injection Vol. : % Aqueous Acetonitrile Solution :.0 ml/min : 40 C : µl min Shimpack XRODS III (0 mml. mmi.d.,.6 µm, P/N: 8999) Shimpack XRODS III (0 mml. mmi.d.,. µm, P/N: 8999) RT of Peak = 0.9 min NTP of Peak =, min min Mobile Phase Flow Rate Col. Temp. Injection Vol. : % Aqueous Acetonitrile Solution :. ml/min : 40 C : 0. µl Mobile Phase Flow Rate Col. Temp. Injection Vol. : % Aqueous Acetonitrile Solution : 0.6 ml/min : 0 C : 0. µl

13 Product Information Shimpack XRODS Shimpack XRC8 Shimpack XRPhenyl Shimpack XRSil Shimpack XRODS II Shimpack XRODS III Length (mm) Pressure Tolerance (MPa) 60 0 Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

14 UHPLC/HPLC s Shimpack VP Series Shimpack VP series is designed for the development and validation of analytical methods. Pore Size (nm) Surface Area (m/g) Carbon Loading Pressure Tolerance (MPa) Pore Volume (ml/g) Endcapping Bonding Type ph Range USP Code Shimpack VPODS 4 % Approx.. Yes Monomeric 7. L Shimpack VPC8 4.% Approx.. Yes Monomeric 7. L7 Shimpack VPPhenyl 4.% Approx.. Yes Monomeric 7. L Strict Manufacturing Uniformity Shimpack VP series ensures high columntocolumn performance reproducibility, which is ideal for method development and validation. Silicabases, surface treatment and packing procedures are subjected to a strict array of quality criteria tests and controlled respectively. Each column is delivered together with Certificate of Compliance and Performance Report. Peaks. Nacetylprocainamide. Phenol, Batch C Batch B Batch A 0 Shimpack VPODS Batch C Batch B Batch A 0 Other brand column Comparison of reproducibility between three batches of silicabased materials Balance of Number of Theoretical Plates and Pressure Shimpack VP series achieves a higher number of theoretical plates while maintaining low pressure. It provides superior performance shown by NTP per MPa. A B C Shimpack VPODS NTP per MPa Size: 4.6 x 0 mm, Mobile Phase: Methanol/Water=70/0, Flow Rate:.0 ml/min., Sample: Naphthalene 4

15 Excellent Peak Shape Peaks. Oxine Peaks : propranolol : acenaphthene : amitriptyline 0 min. 0 min. Shimpack VPODS Other brand column Packing materials with less metal impurities achieve excellent peak shape of coordination compounds min min. Shimpack VPODS Other brand column Completed endcapping achieves excellent peak shape of basic compounds. Product Information Analytical s Shimpack VPODS Shimpack VPC8 Shimpack VPPhenyl Length (mm) Cartridge Guard s Guard.0 Length (mm) Guard Holder GVPODS Cartridges ( pcs) Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

16 UHPLC/HPLC s Shimpack VP Series Smooth Transfer of Methods from HighSpeed Analysis to Conventional Analysis By using the Shimpack VP series of conventional columns in combination with the Shimpack XR series of highspeed columns, which offer the equivalent separation performance, it is possible to perform method development via highspeed analysis, and then smoothly transfer to conventional analysis. Utilizing these columns with Nexera Method Scouting provides strong support for method development. Example of HighSpeed/Conventional Analysis Using s with Equivalent Retention Behavior Examples of analysis with the Shimpack VP and Shimpack XR columns are shown below. In each example, analysis was achieved while maintaining essentially the same relative retention time. In method transfer, this equivalency is important, making the combination of the Shimpack VP series and Shimpack XR series optimal for method development. Example of the Batch Analysis of 6 Sulfa Drugs 4 : Top: Shimpack VPODS (0 mml. 4.6 mmi.d., µm) (P/N: 84979) Bottom: Shimpack XRODS (0 mml..0 mmi.d.,. µm) (P/N: ) Mobile Phase : A) 0. % formic acid B) Acetonitrile A/B = 8/ (v/v) Flow Rate : Top:.0 ml/min Bottom:.0 ml/min Relative Retention Times Peak Shimpack VPODS Shimpack XRODS 4. Sulfadiamine Sulfamerazine..8. Sulfadimidine Sulfamethoxazole Sulfaquinoxaline Example of the Analysis of FatSoluble Vitamins Mobile Phase Flow Rate : Top: Shimpack VPC8 (0 mml. 4.6 mmi.d., µm) (P/N: 89979) Bottom: Shimpack XRC8 (0 mml..0 mmi.d.,. µm) (P/N: 89909) : Methanol : Top:.0 ml/min Bottom:.0 ml/min Relative Retention Times Peak Shimpack VPC8 Shimpack XRC8. Retinol α±tocopherol.09. 6

17 Example of the Batch Analysis of NonSteroidal AntiInflammatory Drugs (NSAIDs) 6 4 : Top: Shimpack VPPhenyl (0 mml. 4.6 mmi.d., µm) (P/N: 89989) Bottom: Shimpack XRPhenyl (0 mml..0 mmi.d.,. µm) (P/N: ) Mobile Phase : A) mmol/l Phosphoric acid (Na) buffer (ph.) B) Acetonitrile A/B = /7 (v/v) Flow Rate : Top:.0 ml/min Bottom:.0 ml/min Relative Retention Times 4 6 Peak, Piroxicam, Ketoprofen, Diflunisal 4, Diclofenac, Indomethacin 6, Ibuprofen Shimpack VPPhenyl Shimpack XRPhenyl s with the same size but different functional groups are available as a set for the purpose of method development. Please contact your local Shimadzu representative for details. Check out the Nexera Method Scouting System at Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 7

18 UHPLC/HPLC s Shimpack MAqCODS Shimpack MAqCODS I reversedphase columns are packed with a silica gel containing metal and added octadecylsilyl group. In addition to the hydrophobic characteristics of the ODS, the metal content also provides cationexchange effects. This increases the retention of basic compounds. Therefore, this allows use with only a buffer solution as the mobile phase for analyses that previously required using an ion pair reagent and enables using gradient elution. These characteristics are especially beneficial for analyzing water soluble vitamins and pharmaceuticals that contain a large amount of basic compounds. Example of Simultaneous Analysis of Water Soluble Vitamins Water soluble vitamins contain many highly polar basic components, which are known to exhibit weak retention in the reversedphase mode. Consequently, with typical ODS columns, such as the Shimpack VPODS, an ion pair reagent is added to the mobile phase for analysis. However, using an ion pair reagent makes gradient elution difficult, resulting in peak broadening for components that take longer to elute and making it difficult to improve sensitivity. In addition, the effort required to prepare the mobile phases and condition the column is also an issue. However, because the Shimpack MAqCODS I enables using gradient elution, it can shorten analysis times and result in sharp peaks even for components that elute slowly. For example, riboflavin, which elutes as the final peak with a typical ODS column, is detected with approx.. times higher sensitivity by the Shimpack MAqCODS I. Shimpack MAqCODS I Shimpack VPODS min Mobile Phase Flow Rate Col. Temp. Detection Injection Vol Peaks. Thiamine. Niacin. Nicotinamide 4. Pyridoxine. Ca Pantothenate 6. Folic acid 7. Riboflavin 8. Biotin : Shimpack MAqCODS I (0 mml. 4.6 mmi.d., µm) (P/N: 89969) : A) mmol/l phosphate (Na) buffer solution (ph.6) B) Acetonitrile A/B = 99/.min 99/ 7.min 0/0 0.0min 99/ min :. ml/min : 40 C : UV nm : µl min Mobile Phase Flow Rate Col. Temp. Detection Injection Vol. 6 8 : IShimpack VPODS (0 mml. 4.6 mmi.d., µm) (P/N: 84979) : A) 0 mmol/l phosphate (Na) buffer solution (ph.) containing 0.8 mmol/l sodium octanesulfonate B) Acetonitrile A/B = / (v/v) :. ml/min : 40 C : UV nm : µl 7 Example of Analyzing Impurities in a Pharmaceutical Many pharmaceuticals are basic compounds. The majority of impurities in pharmaceuticals, such as unreacted ingredients, byproducts, and decomposition products, are highly polar basic substances. Consequently, analyzing impurity peaks using LC/MS can be difficult if a nonvolatile ion pair reagent is contained. In the following example of analyzing famotidine, using a CoSense for LC/MS automatic pretreatment system to desalt the mobile phase used with the Shimpack MAqCODS I column enabled analysis by LC/MS. While a typical ODS column (Shimpack VPODS) detects types of impurities, the Shimpack MAqCODS I detects types of impurities due to separation specificity and gradient elution. Shimpack MAqCODS I Shimpack VPODS Famotidine Enlarged view Famotidine Enlarged view min min 0 min : Shimpack MAqCODS I (0 mml. 4.6 mmi.d., µm) (P/N: 89969) Mobile Phase : A) mmol/l phosphate (Na) buffer solution (ph.6) B) Acetonitrile A/B = 9/8 min 9/8 7min 0/0 0.0min 9/8 8 min Flow Rate :.0 ml/min Col. Temp. : C Detection : UV 4 nm Injection Vol. : µl 0 min Mobile Phase Flow Rate Col. Temp. Detection Injection Vol. : Shimpack VPODS (0 mml. 4.6 mmi.d., µm) (P/N: 84979) : g of sodium heptanesulfonate was dissolved in 900 ml of water and acetic acid (0) was added to produce a ph of.0. Then water was added to make 00 ml. 40 ml of acetonitrile and 40 ml of methanol were added to this solution. : 0. ml/min : C : UV 4 nm : µl 8

19 Example of Analyzing a Cold Remedy Gradient elution with a Shimpack MAqCODS I column was used for simultaneous analysis of components contained in an overthecounter commercial cold remedy. The ability to use gradient elution enables the acquisition of sharp peaks, even for components that eluted slowly, similar to the water soluble vitamin and drug impurity examples on the prior page. * * Mobile Phase Flow Rate Col. Temp. Detection Injection Vol. : Shimpack MAqCODS I (0 mml. 4.6 mmi.d., µm) (P/N: 89969) : A) mmol/l phosphate (Na) buffer solution (ph.) B) Acetonitrile A/B = 99/ min 99/ 6min 0/0 min 0/0 0.0min 99/ min :.0 ml/min : 40 C : UV nm : µl min Peaks. Thiamine * Nitric acid. Acetaminophen * Maleic acid. Caffeine 4. Chlorpheniramine. Ethenzamide 6. Isopropylantipyrine 7. Ibuprofen More Free Literature at Product Information Length (mm) * To use this column efficiently: ) To increase the retention of basic compounds, please use a buffer solution within the ph to 4 range. ) In the case of a basic substance tailing, it may be possible to improve the peak shape by increasing the salt concentration of a buffer solution. ) The elution of basic compounds is faster by increasing the salt concentration, and it is possible to adjust retention by salt concentration. This product developed by collaboration with Eisai Co., Ltd. Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 9

20 UHPLC/HPLC s Shimpack GIST C8 Ultra High Inertness, High Durability Shimpack GIST C8 has superior inertness, which improves analysis precision and increases column durability. In addition, it can be used to analyze strong ionic compounds and difficult to absorb samples, which helps to obtain symmetrical peaks and high reproducibility. Furthermore, because of the new silica gel, Shimpack GIST C8 is suitable for wider ph () analysis at a consistent performance. This enables use of a silicabased column under alkaline conditions. Analysis Examples Bonded Phase Pore Size Surface Area Carbon Loading Endcapping ph Range USP Code Octadecyl Groups µm, µm, µm nm 0 m /g 4 % Yes to L Nine Pesticides on a Golf Course Peaks ( mg/l each). Asulam. Oxine Copper. Triclopyr 4. Mecoprop (MCPP). Thiram 6. Flazasulfuron 7. Halosulfuron Methyl 8. Siduron 9. Azoxystrobin Nucleoside and Nucleic acid base Peaks ( mg/l each). Cytosine. Uracil. Guanine 4. Adenine. Cytidine 6. Uridine 7. Thymine 8. Adenosine 9. Guanosine. Thymidine Time (min) 0 0 Time (min) : Shimpack GISTHP C8 (0 mml..0 mmi.d., µm) (P/N: ) Mobile Phase : A) 0 mm KH PO 4 (ph., H PO 4 ) B) CH CN A/B = 60/40 4 min 40/60 Flow Rate :. ml/min Col. Temp. : 40 ºC Detection : UV nm Injection Vol. :.0 µl : Shimpack GIST C8 (0 mml. 4.6 mmi.d., µm) (P/N: ) Mobile Phase : 0. M NH PO 4, 0. M NaClO 4 (ph.0, H PO 4 ) Flow Rate :.0 ml/min Col. Temp. : 40 ºC Detection : UV 60 nm Injection Vol. : µl Analytical s Length (mm)

21 Cartridge Guard s Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Analytical s (HighPressure Series) Length (mm) *For UHPLC fittings, please refer to page Pressure Tolerance (MPa) Cartridge Guard s (HighPressure Series) Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Pressure Tolerance (MPa) 80 Pressure Tolerance (MPa) 80 Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

22 UHPLC/HPLC s Shimpack GISS C8 Ideal for Rapid Analysis Shimpack GISS C8 maintains the same ultrahigh inertness and wider ph range as the Shimpack GIST C8, while providing rapid separations with symmetrical peaks. The optimization of surface area, pore size and chemical bonding delivers superior peak shapes. It is ideal for LC/MS/MS analysis and enables MScompatible buffers to be used due to extremely inert silica gel. Bonded Phase Pore Size Surface Area Carbon Loading Endcapping ph Range USP Code Octadecyl Groups.9 µm, µm, µm nm 0 m /g 9 % Yes to L Analysis Example βblocker 4 Peaks (0 µg/l each) Q > Q. Acebutolol : 7. > 6.0 (+). Atenolol : 67. > 4.00 (+). Labetalol : 9.00 > 6.9 (+) 4. Nadolol :.0 > 4.00 (+). Pindolol : > 6.00 (+) min min : Shimpack GISS C8 (0 mml. mmi.d.,.9 µm) (P/N: ) Mobile Phase : A) mm Ammonium Formate in H O B) mm Ammonium Formate in CH OH A/B = 70/0 0. min 40/60 0. min 0/0 0. min 0/0 0.0 min 70/0 0. min 70/0, v/v Flow Rate : 0.6 ml/min Col. Temp. : 40 ºC Detection : LC/MS/MS (ESI, Positive, Negative MRM) Analytical s Length (mm)

23 Cartridge Guard s Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Analytical s (HighPressure Series).9 Length (mm) *For UHPLC fittings, please refer to page Pressure Tolerance (MPa) Cartridge Guard s (HighPressure Series).9.9 Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Pressure Tolerance (MPa) 80 Pressure Tolerance (MPa) 80 Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

24 UHPLC/HPLC s Shimpack GIS C8 High Retentivity, Lower Back Pressure Widely used octadecyl bonded silica gel enables the Shimpack GIS C8 to have strong hydrophobic interaction and low absorption of ionic compounds. In addition, highly uniform particles ensure stable mobile phase delivery and outstanding low pressure. Shimpack GIS C8 is ideal for preparative separations. Higher surface area silica and strong retentivity provide high preparative loading capacity without sacrificing peak shape. Analysis Example Bonded Phase Pore Size Surface Area Carbon Loading Endcapping ph Range USP Code Octadecyl Groups µm, µm, 4 µm, µm, µm nm 40 m /g % Yes to 7. L Food Dyes Time (min) Peaks. Tartrazine. Amaranth. Ingigocarmine 4. New coccine. Sunset Yellow FCF 6. Naphthol Yellow S 7. Uranine 8. Allura Red AC 9. Ponceau R 7.6 mg/l.8 mg/l 7.6 mg/l.8 mg/l. mg/l 7.6 mg/l.8 mg/l. mg/l 7.6 mg/l. Ponceau SX. Orange I. Fast green FCF. Brilliant blue FCF 4. Ponceau R. Erythrosine 6. Azure Blue VX 7. Orange II 8. Acid red. mg/l. mg/l.0 mg/l.0 mg/l 7.6 mg/l. mg/l.0 mg/l 7.6 mg/l.0 mg/l : Shimpack GIS C8 (0 mml. 4.6 mmi.d, 4 µm) (P/N: 70007) Mobile Phase : A) mm Na HPO 4 (ph6.9 ; mm NaH PO 4 ) B) CH CN A/B = 90/ 0 min 6/ Flow Rate :.0 ml/min Detection : UV 70 nm Col. Temp. : 40 ºC Injection Vol. : µl Analytical s 4 Length (mm)

25 Analytical s Length (mm) Cartridge Guard s 4 4 Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Analytical s (HighPressure Series) Length (mm) Pressure Tolerance (MPa) *For UHPLC fittings, please refer to page 7. Cartridge Guard s (HighPressure Series) Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Pressure Tolerance (MPa) 80 Pressure Tolerance (MPa) 80 Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

26 UHPLC/HPLC s Shimpack GIST C8 UltraHigh Inertness, High Durability Shimpack GIST C8 is packed with highpurity porous spherical silica for delivering the same extreme inertness to elute either basic or acidic compounds without undesired adsorption. Low retentivity and no sample adsorption enable analysis of natural samples. Shimpack GIST C8 is the ideal choice for the rapid analysis of hydrophobic compounds. Analysis Examples Bonded Phase Pore Size Surface Area Carbon Loading Endcapping ph Range USP Code Octyl Group µm, µm, µm nm 0 m /g 8 % Yes to L7 Pyridine derivatives Peaks ( mg/l each). Pyridine. αpicoline. γpicoline 4. βpicoline.,6lutidine 6.,4Lutidine 7.,Lutidine Phospholipids Peaks. PhosphatidylLSerine (PS) µg/ml Q: 788. Q: 8.0. LαPhosphatidylDLglycerol, Distearoyl (PG) 0. µg/ml Q: Q: 8.. LαPhosphatidylcholine (PC) (0.0 µg/ml) 0.0 µg/ml Q: 806. Q: LαPhosphatidylethanolamine, Distearoyl (PE) 0. µg/ml Q: Q: 8. (in CHCl /CH OH = 0/0, v/v) Time (min) min : Shimpack GIST C8 (0 mml. 4.6 mmi.d., µm) (P/N: 70707) Mobile Phase : A) mm Na HPO 4 (ph 8.0, H PO 4 ) B) THF A/B = 87/, v/v Flow Rate :.0 ml/min Col. Temp. : 40 ºC Detection : UV 60 nm Injection Vol. : µl : Shimpack GIST C8 (0 mml.. mmi.d., µm) (P/N: ) Mobile Phase : 0. % HCOOH, mm HCOONH 4 in CH OH Flow Rate : 0. ml/min Col. Temp. : 40 ºC Detection : LC/MS/MS (ESI, Negative, MRM) Injection Vol. : µl Analytical s Length (mm)

27 Cartridge Guard s Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Analytical s (HighPressure Series) Length (mm) *For UHPLC fittings, please refer to page Pressure Tolerance (MPa) Cartridge Guard s (HighPressure Series) Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Pressure Tolerance (MPa) 80 Pressure Tolerance (MPa) 80 Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 7

28 UHPLC/HPLC s Shimpack GIST Phenyl Extremely Strong ππ Interactions The extremely unique phase characteristics of Shimpack GIST Phenyl are critical to resolving compounds that could not be separated with a C8 or C8 phase column. In addition to ππ interactions, Shimpack GIS Phenyl provides hydrogen bonding secondary interactions, which results in retaining polar compounds at the same time. As the phenyl groups are directly bonded to the silica gel, Shimpack GIS Phenyl is also capable of the analysis of structural isomers due to its high stereoselectivity. Bonded Phase Pore Size Surface Area Carbon Loading Endcapping ph Range USP Code Phenyl Group µm, µm, µm nm 0 m /g % to 7. L Analysis Example Steroidal antiinflammatory drug Peaks (0. mg/l each). Hydrocortisone. Prednisolone Time (min) : Shimpack GIST Phenyl (0 mml.. mmi.d., µm) (P/N: 7070) Mobile Phase : A) H O/HCOOH = 0/0.0, v/v B) CH OH/HCOOH = 0/0.0, v/v A/B = 60/40, v/v Flow Rate : 0.6 ml/min Col. Temp. : 40 ºC Detection : LC/MS/MS (ESI, Positive, MRM) Injection Vol. : µl Analytical s Length (mm)

29 Cartridge Guard s Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Analytical s (HighPressure Series) Length (mm) *For UHPLC fittings, please refer to page Pressure Tolerance (MPa) Cartridge Guard s (HighPressure Series) Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Pressure Tolerance (MPa) 80 Pressure Tolerance (MPa) 80 Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 9

30 UHPLC/HPLC s Shimpack GIST NH Suitable for Sugar Analysis New highpurity porous spherical silica chemically bonded with the aminopropyl group ensures the superior stability of Shimpack GIST NH. It is capable of the analysis of vitamin E or simultaneous analysis of sugars that are hard to separate in reversed phase mode. In addition, due to being primarily aminebound, Shimpack GIST NH can analyze sugars with no separation of anomers, even under lowtemperature conditions. Furthermore, Shimpack GIST NH delivers highly reliable reproducibility and stability with accurate qualitative and quantitative results. Bonded Phase Pore Size Surface Area Carbon Loading Endcapping ph Range USP Code Aminopropyl Group µm, µm nm 0 m /g 7 % to 7. L8 Analysis Examples Sugars 4 Peaks. Fructose. Glucose. Sucrose 4. Maltose Time (min) : Shimpack GIST NH ( mml. 4.6 mmi.d., µm) (P/N: 7008) Mobile Phase : A) H O B) CH CN A/B = /7, v/v Flow Rate :.0 ml/min Col. Temp. : 40 ºC Detection : RID Injection Vol. : µl Vitamin E 4 Peaks ( mg/l each). αtocopherol. βtocopherol. γtocopherol 4. δtocopherol Time (min) : Shimpack GIST NH ( mml. 4.6 mmi.d., µm) (P/N: 7008) Mobile Phase : A) nhexane B) Ethyl acetate A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 ºC Detection : UV 90 nm Injection Vol. : µl 0

31 Analytical s Length (mm) Cartridge Guard s Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

32 UHPLC/HPLC s Shimpack GIS CN Suitable in Either Reversed Phase or Normal Phase Mode Shimpack GIS CN is capable of either normal phase or reversed phase analysis. Cyanopropryl groups bonded to silica gel with high density increases the difference recognition of hydrophilicity and the durability. Due to no endcapping, it is capable of analysis utilizing cyano group characteristics. In reversed phase mode, separation can be achieved for those compounds that could not be separated on straightchainalkyl columns, such as C8 or C8 bonded phases. When using the column for reversed phase mode, fully equilibrate the column before use. Bonded Phase Pore Size Surface Area Carbon Loading Endcapping ph Range USP Code Cyanopropryl Group µm, µm nm 40 m /g 4 % to 7. L Analysis Examples gammaoryzanol Peaks. gammaoryzanol. vanillin Time (min) 4 : Shimpack GIS CN (0 mml. 4.6 mmi.d, µm) (P/N: 70606) Mobile Phase : hexane:propanol:acetic acid = 94:: Flow Rate :.0 ml/min Col. Temp. : 0 ºC Detection : UV nm Injection Vol. : µl Free Amino acids and Organic acids Time (min) Peaks. Asparagine H O (0.7 mg/ml). Aspartic acid (0.7 mg/ml). Fumaric acid (0.0 mg/ml) 4. Maleic acid (0.0 mg/ml) : Shimpack GIS CN ( mml. 4.6 mmi.d., µm) (P/N: 70607) Mobile Phase : mm KH PO 4 (ph 4.0; H PO 4 ) Flow Rate :.0 ml/min Col. Temp. : 40 ºC Detection : UV nm Injection Vol. : µl

33 Analytical s Length (mm) Cartridge Guard s Cartridge Guard (pcs) Length (mm) Cartridge Guard (pcs) and Holder Length (mm) Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

34 UHPLC/HPLC s Shimpack GWS C8 Economical Choice Shimpack GWS C8 is packed with highpurity silica gel. A uniform pore size ensures low pressure, while complete endcapping makes it possible for analysis of acidic or basic compounds. Shimpack GWS series is an ideal choice for cost control. Bonded Phase Pore Size Surface Area Carbon Loading Endcapping ph Range USP Code Octadecyl Groups µm nm 40 m /g 9. % Yes to 7. L Analysis Examples Cold medication 4 Puerariae radix Peaks ( mg/l each). Potassium GuaiacoI sulfonate. Acetaminophen. Caffeine 4. Ethenzamide. Allyl isopropyi acetyi urea 6. Isopropylantipyrine 7. Ibuprofen 6 7 Peaks. Puerarin Time (min) Time (min) : Shimpack GWS C8 (0 mml. 4.6 mmi.d., µm) (P/N: 7080) Mobile Phase : A) 0.%H PO 4 B) CH CN A/B = 90/ min 90/ min 0/0 Flow Rate :.0 ml/min. Col. Temp. : 40 ºC Detection : UV nm Injection Vol. : µl : Shimpack GWS C8 ( mml. 4.6 mmi.d., µm) (P/N: 7080) Mobile Phase : A) H O B) CH CN A/B = 89/ Flow Rate :.0 ml/min. Col. Temp. : ºC Detection : UV nm Injection Vol. : µl Analytical s Length (mm) Cartridge Guard Length (mm) Cartridge Guard (pcs) 4.0 Cartridge Guard (pcs) and Holder

35 Shimpack FCODS Shortens the Analysis Time Using a Conventional System Shimpack FCODS is an ideal column to shorten your analysis time using conventional HPLC. Its innovative surface structure and optimized packing method also enable outstanding resolution. Particle size is µm, but the performance of a Shimpack FCODS is equivalent to a µm column while the resolution is twice as that of a µm column. Therefore, Shimpack FCODS can not only shorten analysis times, but also provide a higher number of theoretical plates. Pore Size Surface Area Carbon Loading Pressure Tolerance Pore Volume Endcapping Bonding Type ph Range USP Code µm nm m/g 8% MPa ml/g Yes Monomeric. 9 L Analysis Examples Shimpack FCODS separates components by hydrophobic interaction like other ODS columns. It is possible to change to Shimpack FCODS from other ODS columns under the same analytical conditions. On the other hand, hydrophilic interaction (hydrogen bond, coordination bond) has been restricted to a minimum, which ensures significant efficiency when analyzing basic compounds. In addition, Shimpack FCODS has higher steric selectivity (capability to recognize the difference of steric structures), making it possible to separate some components that are difficult to retain in other ODS columns. Shimpack FCODS is available in three lengths to suit analysis objectives. 0mm Recommended for use in highthroughput analysis of samples that do not have a complex matrix. 7mm Recommended for shortening the analysis time to that of a 0 mm column. Because Shimpack FCODS retains a similar number of theoretical plates as a 0 mm column, it is possible to obtain the same result within about half of the time without changing the conditions. (In the case of gradient analysis, it is necessary to change the concentration.) 0mm Recommended for analyzing samples that are difficult to be retained in other 0 mm ODS columns. HighThroughput Analysis times repeated analysis in 6. minutes is possible. : Shimpack FCODS (0 mml. 4.6 mmi.d., µm) (P/N: 8409) Mobile Phase : Water/Acetonitrile = /4 (v/v) Flow Rate :.0 ml/min ( Pressure ca.8mpa) Col. Temp. : 0 ºC Detection : 4 nm Response, AuxRNG Instrument : LC+CR8A Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

36 UHPLC/HPLC s Shimpack FCODS Highspeed analysis of,4dnph derivatized aldehydes / ketones Mobile Phase Flow Rate Col. Temp. Detection : Shimpack FCODS (7 mml. 4.6 mmi.d., µm) (P/N: 8409) : Water/Acetonitrile = /4 (v/v) :.0 ml/min : 40 C : UV 60 nm Chromatogram of Standard Sample (formaldehyde 0.µg/mL, acetaldehyde 0.µg/mL, µl injected) Chromatograms of Environmental Air (A) and Indoor Air at Laboratory (B) Mobile Phase Flow Rate Col. Temp. Detection : Shimpack FCODS (7 mml. 4.6 mmi.d., µm) (P/N: 8409) : A) Water/Tetrahydrofuran = 8/ (v/v) B) Acetonitrile A/B = 80/ 4min 40/60 0.0min 80/ 6min :. ml/min : 40 C : UV 6 nm Chromatogram of Standard Sample (each 0.µg/mL as carbonyl compounds, µl injected) Chromatograms of Indoor Air at Laboratory (A) and Operation blank (B) Product Information Length (mm)

37 SFC s Shimpack UCX Series Packed s for Supercritical Fluid Chromatograph When conducting analysis with the Nexera UC supercritical fluid chromatography system, because diffusion of the sample band in the mobile phase is high compared with liquid chromatography, separation behavior changes significantly depending on the types of columns used. Shimpack UCX series was designed with a variety of stationary phases, making it suitable for analysis of various compounds. Achieve high speed and high performance Shimpack UCX series offers various stationary phases suitable for the mobile phase of high diffusion and lowviscosity liquid carbon dioxide. Analysis time can be reduced at a high flow rate without impairing the separation performance. In addition, analysis speed, which is limited in HPLC, can be increased. Wide range of stationary phases meets diverse needs Shimpack UCX series was designed with eight types of stationary phases and sizes to meet diverse research and development needs. High durability and stable reproducibility Shimpack UCX series achieves high inertness for improved analysis precision and increased column durability. Its uniform silica surface and stable chemical modification also ensure high analysis reproducibility. Shimpack UCX RP Shimpack UCX GIS II Shimpack UCX Phenyl Shimpack UCX CN Bonded Phase Octadecyl Groups + Polar Functional Group Octadecyl Groups Phenyl Groups Cyanopropryl Groups,,,, Pore Size (nm) Surface Area (m/g) Carbon Loading 9% % 9.% 4% Pressure Tolerance (MPa) Pore Volume (ml/g) Endcapping Yes ph Range USP Code L L L L Shimpack UCX Diol Shimpack UCX Sil Shimpack UCX Amide Shimpack UCX NH Bonded Phase Diol Group Carbamoyl Groups Aminopropyl Groups,,,, Pore Size (nm) Surface Area (m/g) Carbon Loading % 8% 8% Pressure Tolerance (MPa) Pore Volume (ml/g) Endcapping ph Range USP Code L L L8 Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 7

38 SFC s Shimpack UCX Series Analysis Examples Tocopherol Isomers Isomers and structurally similar compounds that are difficult to separate can be analyzed by Nexera UC and Shimpack UCX series at a high speed. In the example below, four kinds of tocopherol isomers were analyzed by Nexera UC and Shimpack UCX Sil. As shown below, analysis time was reduced to onethird the time analysis takes with a conventional HPLC method while resolution was improved. Phospholipid Classes Peaks. Phosphatidyl Choline. Lyso Phosphatidyl Choline. Phosphatidyl Ethanolamine 4. Lyso Phosphatidyl Ethanolamine. Phosphatidyl Glycerol 6. Phosphatidyl Inositol HPLC Rs = min SFC Approx. / Rs = min The example above shows the result of the analysis of phospholipid classes using Shimpack UCX Diol with Nexera UC. The ODSbased Shimpack UCX GIS II can also be used to analyze molecular species of phospholipids with the same modifiers. Nexera UC and Shimpack UCX series are ideal for analyzing complex samples. 0 4 min Comparison of retention and resolution of tocopherol isomers between LC conditions and SFC ones Multiple Pesticides with a Wide Polarity Range Simultaneous analysis of multiple pesticides with a wide polarity range from hydrophobic to hydrophilic has been achieved using the Nexera UC and Shimpack UCX RP. Shimpack UCX RP contains a polar functional group embedded between the silica surface and the C8 group. This allows the column to analyze a wide range of components from hydrophobic to hydrophilic ones. Simultaneous analysis of multiple components can now be performed more efficiently using the Nexera UC and Shimpack UCX series. No. Components log P Carbofuran Ethofenprox Fenpropathrin Pyriproxyfen Pyraclostrobin Linuron Aminocarb Ethoxysulfuron Halosulfuron methyl Bentazone Chlorsulfuron Rimsulfuron Nicosulfuron Vamidothion hydrophobic hydrophilic min 8

39 Product Information Length (mm). 4.6 Shimpack UCX RP Shimpack UCX GIS II Shimpack UCX Phenyl Shimpack UCX CN Shimpack UCX Diol Shimpack UCX Sil Shimpack UCX Amide Shimpack UCX NH Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 9

40 Size Exclusion s Shimpack GPC Series Shimpack GPC series columns are used for the determination of tetrahydrofuran (800 Series), chloroform (800C Series), and dimethylformamide (800D series). The technique of GPC does not utilize such chemical reactions as partition, adsorption, and ion exchange, but a physical reaction consisting of a separation based on molecular size of the sample components. Therefore, this method is suitable for the measurement of molecular weight distribution of high polymers and oligomers. Shimpack GPC series are packed with polystyrene polymers with respective degrees of crosslinking in order to meet exact analysis requirements, ranging from analysis of high polymers to that of oligomers. GPC80M (80MC, 80MD) are mixed gel columns. Determination of Tetrahydrofuran Analytical s Exclusion Limit (polystyrene) Dimensions (Length I.D., mm) P/N Shimpack GPC Shimpack GPC Shimpack GPC Shimpack GPC Shimpack GPC Shimpack GPC Shimpack GPC Shimpack GPC80M 4 7, Mixed gel Guard s Guard Shimpack GPC800P Dimensions (Length I.D., mm) 4.6 P/N 889 Determination of Chloroform Analytical s Exclusion Limit (polystyrene) Dimensions (Length I.D., mm) P/N Shimpack GPC80C Shimpack GPC80C Shimpack GPC80C Shimpack GPC80C Shimpack GPC804C Shimpack GPC80C Shimpack GPC806C Shimpack GPC80MC 4 7, Mixed gel Guard s Guard Shimpack GPC800CP Dimensions (Length I.D., mm) 4.6 P/N

41 Determination of Dimethylformamide Analytical s Exclusion Limit (polystyrene) Dimensions (Length I.D., mm) P/N Shimpack GPC80D Shimpack GPC80D Shimpack GPC80D Shimpack GPC80D Shimpack GPC804D Shimpack GPC80D Shimpack GPC806D Shimpack GPC80MD 4 7, Mixed gel Guard s Analysis Examples Calibration Curves Guard Shimpack GPC800DP Dimensions (Length I.D., mm) 4.6 P/N 889 Analysis of Polystyrene Standard Mobile Phase Flow Rate Col. Temp. Detection : Tetrahydrofuran :.0 ml/min : Ambient : UV 4 nm Peaks. Polystyrene M.w. 9,K. Polystyrene,0K. Polystyrene 4K 4. Polystyrene K. Polystyrene K 6. Polystyrene 7.6K 7. Polystyrene.K 8. npropylbenzene Mobile Phase Flow Rate Col. Temp. Detection : Shimpack GPC80M ( columns in series) (P/N: 889) : Tetrahydrofuran :.0 ml/min : Ambient : UV 4 nm Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 4

42 Size Exclusion s Shimpack Diol Series Shimpack Diol series is a kind of gel filtration chromatography (GFC) column. GFC is used to separate watersoluble high polymers such as polysaccharides, proteins, and nucleic acids according to their molecular sizes by using hydrophilic packing materials and aqueous mobile phase. Shimpack Diol series is packed with porous spherical silica gel chemically bonded with a hydroxyl group. Due to the hydrophilic hydroxyl group, Shimpack Diol series can be used in highspeed GFC and provide sharp peaks during the analysis of protein and biochemicals (such as enzymes). Product Information Stationary Phase Dimensions (Length I.D., mm) P/N Shimpack Diol0 Diol group Shimpack Diol00 Diol group Precolumn Diol * Diol group * Installed between the liquid pump and the sample injector to protect the Shimpack Diol column. Analysis Examples Calibration Curves Peaks. Blue dextran 00. Thyroglobulin. yglobulin 4. Transferrin. Human serum albumin 6. Ovalbumin 7. βlactoglobulin 8. Chymotrypsin 9. Myoglobin. Lysozyme. Ribonuclease A. Cytochrome C. GlyTyr Mobile Phase Flow Rate Col. Temp. Detection : Shimpack Diol Series (00 mml 7.9 mmi.d., µm) : A) mm phosphate buffer solution (ph 7) B) 0.M sodium sulfate :.0 ml/min : Ambient : UV 80 nm Analysis of Protein Standard Peaks. Glutamate dehydrogenase. Lactate dehydrogenase. Enolase 4. Adenylate kinase. Cytochrome C Mobile Phase Flow Rate Col. Temp. Detection : Shimpack Diol00 (00 mml 7.9 mmi.d., µm) (P/N: ) : A) mm phosphate buffer solution (ph 7) B) 0.M sodium chloride :.0 ml/min : Ambient : UV 80 nm 4

43 Ion Chromatography s Shimpack IC Series Ion chromatography (IC) is used for analysis of inorganic and organic ions. It is categorized as suppressor IC and nonsuppressor IC. Nonsuppressor IC is composed of a conventional HPLC system combined with a conductivity detector, while suppressor IC requires an extra suppressor. NonSuppressor Type Anion Analysis Cation Analysis Shimpack ICA Shimpack ICA Shimpack ICC Shimpack ICC4 Capable of analyzing inorganic anions. Compatible with acid or basic mobile phases of wide ph range. Provides outstanding separation efficiency in the analysis of inorganic anions and acid ions, especially when weakly acid mobile phase is used. Capable of analyzing cations such as alkaline metal ions, alkali earth metal ions, transition metal ions, rare earth metal ions, and alkyl amines. Capable of simultaneous analysis of alkali metal ions and alkaline earth metal ions. Enhances the separation of sodium and ammonium ions. Shimpack ICSA Capable of determining fluoride ions, chloride ions, nitrite ions, bromide ions, nitrate ions, phosphate ions and sulfuric acid ions. Anion Analysis Shimpack ICSA Provides highresolution performance when analyzing fluoride ions, chloride ions, nitrous acid ions, bromide ions, nitric acid ions, phosphate ions and sulfuric acid ions as well as chlorous acid ions, chloric acid ions and bromic acid ions. Suppressor Type Shimpack ICSA4 Capable of analyzing fluoride ions, chloride ions, nitrite ions, bromide ions, nitrate ions, phosphate ions as well as sulfate ions, chlorite ions, chlorate ions and bromate ions in waterquality analysis in approx. minutes. Cation Analysis Shimpack ICSC Also can be used in nonsuppressor IC. Suitable for the analysis of water quality. Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 4

44 Ion Chromatography s Shimpack IC Series Analysis Examples Examples of Cation Analysis Shimpack ICC4 is a cation analysis column for nonsuppressor IC. Because the ph of the mobile phase can be changed by selecting a different combination of acid and base in eluent, nonsuppressor IC enables various kinds of analysis. High Resolution of Na+ and NH4+ High resolution of Na+ and NH4+ has been achieved by improving the peak shape of Na+. The influence on the peak shape of NH4+ from a high concentration of Na+ has been reduced, making it possible to analyze tap water of normal concentration under standard mobile phase conditions. The resolution can be further improved by using a mobile phase treated with 8crown6 additive. Peaks. Na +. NH4 +. K + 4. Mg +. Ca min : Shimpack ICC4 (0 mml 4.6 mmi.d., 7 µm) (P/N: 84669) Mobile Phase :. mmol/l oxalic acid Flow Rate :.0 ml/min Col. Temp. : 40 C Detection : CDD Injection Vol. : 0 µl Analyses of a Standard Mixture of Cations Flexible Mobile Phase Selection Due to the features of nonsuppressor IC, flexible mobile phase composition can be used. Besides normal inorganic cations, Shimpack ICC4 is capable of analyzing transition metals by using a mixed mobile phase. Mobile Phase Flow Rate Col. Temp. Detection Injection Vol. : Shimpack ICC4 (0 mml 4.6 mmi.d., 7 µm) (P/N: 84669) : A). mmol/l oxalic acid B). mmol/l methanesulfonic acid A/B = 60/40 (v/v) :.0 ml/min : 40 C : CDD : 0 µl 44

45 Analysis of Anions in Water Shimpack ICSA4 was developed for anion analysis on suppressor IC. It is capable of analyzing fluoride ions, chloride ions, nitrite ions, bromide ions, nitrate ions, phosphate ions as well as sulfate ions, chlorite ions, chlorate ions and bromate ions in waterquality analysis in approx. minutes. Achieve high resolution while shortening analysis time. With its. µm particle size, Shimpack ICSA4 enables high resolution of multi anions, a requirement in waterquality analysis. Furthermore, sulfate ions, which have strong retentivity, can be separated in about minutes. Separation of Br and CIO has also been improved compared to previous products. Peaks 6. F. Acetic acid. Formic acid 4. ClO. BrO 6. Cl mg/l mg/l mg/l mg/l 0. mg/l 4 mg/l 7. NO mg/l 8. Br mg/l 9. ClO mg/l. NO mg/l. PO4 mg/l. SO4 mg/l : Shimpack ICSA4 (0 mml 4.6 mmi.d.,. µm) (P/N: 89009) Mobile Phase : A).7 mmol/l sodium carbonate B).0 mmol/l sodium hydrogen carbonate aqueous solution Flow Rate : 0.8 ml/min Col. Temp. : 0 C Detection : CDD Injection Vol. : 0 µl * Silicic acid Analysis of Standard Anion Samples Higher determination resolution of the components in waterquality analysis In the analysis of a water sample containing a large amount of silicic acid, fluoride ions can still be separated and determined with high resolution. Cl Cl NO SO4 F Silicic acid Analysis of Tap Water Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 4

46 Ion Chromatography s Shimpack IC Series Product Information Stationary Phase Dimensions (Length I.D., mm) P/N Guard Shimpack ICA Quaternary ammonium group Shimpack ICA Shimpack ICA (S) * Quaternary ammonium group Quaternary ammonium group Shimpack ICC * Sulfone group Shimpack ICC PEEK Sulfone group Shimpack ICC4 Carboxyl group (Cartridge + Holder) (Cartridge only) Shimpack ICSA Quaternary ammonium group Shimpack ICSA Quaternary ammonium group Shimpack ICSA4 Quaternary ammonium group * Shimpack ICSC Carboxylic group * Shimpack IC(S) series are for semimicro LC. PIA00 is required. * During the analysis of alkali metal ions with the Shimpack ICC, it is recommended to use a precolumn Shimpack ICPC (P/N:877449) installed between the liquid pump and the sample injector. * No guard column is required. Please use the following precolumn filter instead. Filter Set (P/N: 80460), Replacement Filter (P/N: 80460) More Free Literature at 46

47 Dedicated s Analysis of Amino Acids Shimpack Amino Series Shimpack Amino series uses polystyrene gel as solid support, making it possible to utilize both electrostatic reaction and hydrophobic reaction. It is ideal for the analysis of amino acids. Analysis of Amino Acid Standard Analysis of Vinegar Peaks Mobile Phase Flow Rate Col. Temp. Detection : Shimpack AminoNa (0 mml 6.0 mmi.d., µm) (P/N: 88879) : Mobile phase kit for amino acid analysis (Na Type) (P/N: 8994) : 0.4 ml/min : 60 C : RF (Postcolumn derivation). ASP. THR. SER 4. GLU. PRO 6. GLY 7. ALA 8. CYSTINE 9. VAL. MET. ILE 6. HIS. LEU 7. LYS. TYR 8. ARG 4. PHE. GABA Analysis of Soy Sauce Mobile Phase Flow Rate Col. Temp. Detection Simultaneous Analysis of 8 Amino Acids : Shimpack AminoLi (0 mml 6.0 mmi.d., µm) (P/N: 88879) : Mobile phase kit for amino acid analysis (Li Type) (P/N: 899) : 0.6 ml/min : 9 C : RFAXL Ex. 0 nm, Em. 40 nm Product Information Stationary Phase Dimensions (Length I.D., mm) P/N Guard Shimpack AMINONA Na type sulfone group * Shimpack AMINOLI Li type sulfone group * Dedicated for the analysis of cyanide. Please do not use it in the analysis of amino acids. In the analysis of amino acids, the following trap columns are required. Description ISC0/S 004 NA (For trapping Na type ammonia) ISC0/S 004 LI (For trapping Li type ammonia) Dimensions (Length I.D., mm) P/N Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 47

48 Dedicated s Analysis of Sugar and Organic Acid Shimpack SCR Series Shimpack SCRN/C/P are suitable for the analysis of monosaccharides. Since the samples are separated under a mixed mode of gel filtration and ligand exchange, the selectivity differs depending on the type of cation. Shimpack SCRH and SCRH are ion exclusion chromatography columns, using H type sulfonated styrene polymer as stationary phase. They are ideal for analysis of organic acids using an acid aqueous solution (e.g. aqueous solution of perchloric acid) as mobile phase. Peaks. PEG Maltose. Glucose 4. Fructose. Glycerol 6. Ethanol 7. Sorbitol Analysis of Saccharide Standard : Shimpack SCRC (00 mml 7.9 mmi.d., µm) (P/N: ) Mobile Phase : Water Flow Rate :.0 ml/min Col. Temp. : 80 C Detection : RID Peaks. Phosphoric acid. αketoglutaric acid. Citric acid 4. Pyruvic acid. Malic acid 6. Succinio acid 7. Lactic acid 8. Formic acid 9. Fumaric acid. Acetic acid. Levulinic acid. Pyroglutamic acid Analysis of Organic Acids : Shimpack SCRH ( columns in series) (P/N: 87899) Mobile Phase : mm ptoluene sulfonic acids aqueous solution Flow Rate : 0.8 ml/min Col. Temp. : 40 C Detection : CDD (ph buffer organic acids analysis system) Product Information Stationary Phase Dimensions (Length I.D., mm) P/N Guard Shimpack SCRN Na type sulfone group Shimpack SCRC Ca type sulfone group Shimpack SCRP Pb type sulfone group Shimpack SCRH H type sulfone group Shimpack SCRH H type sulfone group

49 Shimpack ISA/ISC Series Shimpack ISA/ISC series uses polystyrene gel as solid support, making it possible to utilize both electrostatic reaction and hydrophobic reaction. They are suitable for the analysis of sugars (ISA) and guanidiono compounds (ISC0). Analysis of Saccharide Standard Peaks. Sucrose. Cellobiose. Maltose 4. Lactose. Rhamnose 6. Ribose 7. Mannose 8. Fructose 9. Galactose. Xylose. Giucose Mobile Phase Flow Rate Col. Temp. Detection : Shimpack ISA07/S4 ( mml 4.0 mmi.d., 7 µm) (P/N: ) : Potassium borate buffer solution gradient elution : 0.6 ml/min : 6 C : RF EX. 48 nm, Em. 40 nm (Postcolumn derivatization with arginine) Product Information Stationary Phase Dimensions (Length I.D., mm) P/N Guard Shimpack ISA07/S 4 Quaternery ammonium group Shimpack ISC0/S 004 Shimpack ISC07/S 04 Na type sulfone group Na type sulfone group Shimpack ISC07/S 04 Li Li type sulfone group Check out more Application Systems at Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 49

50 Dedicated s Analysis of Sugar and Organic Acid Shimpack SPR Series Shimpack SPR series has been developed for the analysis of neutral to weakly acid hydrophilic compounds. These columns are ideal for analyzing organic acids such as shortchain fatty acids, hydroxycarboxylic acids and ketocarboxylic acids, as well as trisaccharides or below and sugar alcohols. Shimpack SPRH Ion exclusion chromatography column primarily for analyzing organic acids. Shimpack SPRNa Size exclusion chromatography column for analyzing trisaccharides or below and organic acids. Shimpack SPRCa Size exclusion chromatography column mixed with ligand exchange mode for analyzing trisaccharides or below and sugar alcohols. Shimpack SPR series columns are packed with 8% crosslinked sulfonated polystyrene gel and used under the following conditions. Pressure Tolerance Flow Rate Temperature Pressure Tolerance All 7.8 mmi.d. 4.6 mmi.d. Shimpack SPRH Shimpack SPRNa Shimpack SPRCa Shimpack SPRPb Maximum 4.0MPa.0 ml/min 0. ml/min 6 C 8 C 8 C 8 C Normal.MPa 0.6 ml/min 0. ml/min 0 C 60 C 80 C 80 C Shimpack SPRPb Size exclusion chromatography column mixed with strong ligand exchange mode, wellsuited for analyzing sugar alcohols. Elution Pattern of Sugar SPRNa SPRCa SPRPb Peaks. Raffinose. Maltotriose. Sucrose 4. Maltose. Lactose 6. Gluctose 7. Mannitol 8. Rhamnose 9. Sorbitol. Galactose (Each elution position) Mobile Phase Flow Rate Col. Temp.. Mannose. Fructose. Xylose 4. Xylitol. Inositol 6. Fucose 7. Arabinose 8. Glycerol 9. Ribose. Ethanol : Water : 0.6 ml/min : 80 C * The elution pattern is not a total chromatogram. It is intended to indicate the elution position only. The separation may differ during the analysis. Product Information Stationary Phase Dimensions (Length I.D., mm) P/N Guard Shimpack SPRH H type sulfone group Shimpack SPRCA Ca type sulfone group Shimpack SPRNA Na type sulfone group Shimpack SPRPB Pb type sulfone group

51 Analysis of Nucleotides, Oligonucleotides and Protein Shimpack WAX/WCX Series Shimpack WAX/WCX series columns are chemicallybonded hydrophilic silica gel based ion exchange columns. Shimpack WAX is ideal for analysis of nucleotides and oligonucleotides while Shimpack WAX and WCX is ideal for analysis of proteins. Peaks. UMP. CMP. AMP 4. GMP. UDP 6. CDP 7. ADP 8. UTP 9. GDP. CTP. ATP. GTP Mobile Phase Flow Rate Col. Temp. Detection : Shimpack WAX (0 mml 4.0 mmi.d., µm) (P/N: 869) : A) mm phosphate buffer solution (ph 7) B) 480mM phosphoric acid buffer solution (ph 6.8) gradient elution :.0 ml/min : 4 C : UV 60 nm Analysis of Mononucleotides Peaks. Ovalbumin. Myoglobin. αchymotrypsinogen A 4. Ribonuclease A. Lysozyme Analysis of Protein Standard Mobile Phase Flow Rate Col. Temp. Detection : Shimpack WCX (0 mml 4.0 mmi.d., µm) (P/N: 86669) : A) mm phosphate buffer solution (ph 6.0) B) Sodium sulfate gradient elution :.0 ml/min : Ambient : UV 4 nm Product Information Stationary Phase Dimensions (Length I.D., mm) P/N Shimpack WAX Tertiary amino group Shimpack WAX Tertiary amino group Shimpack WAXT Tertiary amino group Shimpack WAX Tertiary amino group Shimpack WCX Corboxyl group Precolumn Diol * Diol group * Installed between the liquid pump and the sample injector to protect the column. Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

52 Pretreatment s Shimpack MAYI Series Due to optimized particle size and a newly developed coating technology, the MAYI series online pretreatment column is highly effective in deproteinization and offers longterm stability. It provides excellent reproducibility even for continuous analysis of multiple analytes. How the Shimpack MAYI Series Works The outer surfaces of silica gel (0 µm) are coated with a hydrophilic polymer, so that only the interior of pores are chemically modified by octadecyl radicals (ODS). Since proteins and other macromolecules cannot enter the pores and are blocked by the hydrophilic polymer on the outer surfaces, they are quickly eluted without being retained by the ODS solid phase. In contrast, pharmaceuticals and other induced low molecular weight compounds penetrate the pores and are retained by the inner surfaces of the stationary phase. Quick and Reliable Protein Removal The newly developed hydrophilic polymer coating technology quickly and reliably removes macromolecules, such as protein, from injected biological samples to achieve high recovery rates for target components. In addition to securely protecting analytical columns and LC/MS interfaces, this also helps reduce the time required for finishing the analysis. Outstanding Durability Due to the polymer coating technology and particle size optimization, stable data can be obtained for long periods. The figure below shows results from 00 consecutive injections of 0 µl of blood plasma. No decrease in the deproteinization rate or degradation of peak shape was observed. st analysis 00th analysis Valve switching Valve switching Time (min) Time (min) Samples Detection Injection Vol. Comparison of st and 00th Analyses : Isopropylantipyrine added Blood plasma Sample solution: 0.% phosphoric acid and acetonitrile mixture (9:) Dilution: 8 times : Analysis: 7 nm, Blood plasma matrix: 80 nm : 0 µl

53 Stable Trap Even for Large Injection Volumes The Shimpack MAYIODS column provides stable component recovery rates and protein removal, even when injecting large volumes. Even when 00 µl of blood plasma was directly injected, a high recovery rate was obtained and no peak distortion was observed. Samples Detection : Indomethacin added Blood plasma Sample solution: 0.% phosphoric acid and acetonitrile mixture (9:) Dilution: 8 times : UV nm 00 µl Injection 0 µl Injection 0 µl Injection Time (min) Comparison Data for Injecting 0, 0, and 00 µl Product Information Shimpack MAYIODS(G) Shimpack MAYIC4(G) Shimpack MAYIC8(G) Shimpack MAYIC4(G) Shimpack MAYIC(G) Shimpack MAYISCX(G) Shimpack MAYISAX(G) Stationary Phase Octadecyl group Tetradecyl group Octyl group Butyl group Methyl group Sulfonic acid group Trimethylammonium group Separation Mode Strongest retentivity in reversed phase mode Retentivity next to ODS in reversed phase mode Retentivity next to C4 in reversed phase mode Retentivity next to C8 in reversed phase mode Weakest retentivity in reversed phase mode Strong acid cation exchange mode Weakly basic anion exchange mode Cartridge Holder Dimensions (Length I.D., mm) P/N Dimensions (Length I.D., mm) P/N Shimpack MAYIODS Shimpack MAYIC Shimpack MAYIC Shimpack MAYIC Shimpack MAYIC Shimpack MAYISAX Shimpack MAYISCX Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

54 Pretreatment s Shimpack SPC Series Shimpack SPC series is specifically developed for online sample preparation systems that utilize a column switching method. Shimpack SPCRP column is packed with polymer particles and used for reversed phase LC. Shimpack SPCAE column is packed with fully porous silica gel particles on which weakly basic anion exchange functions are chemically bonded. Product Information Stationary Phase Separation Mode Dimensions (Length I.D., mm) P/N Shimpack SPCRP Polymer Reversed phase Shimpack SPCRP Polymer Reversed phase Shimpack SPCAE Tertiary amino group Anion exchange Check out Online Sample Preparation CoSense Series at 4

55 Preparative s Shimpack PREP Series Shimpack PREP Series Shimpack PREP series is packed with fully porous spherical silica particles on which respective stationary phases are chemically bonded. (Except the PREPSIL which is packed with silica particles without any surface treatment.) The residual silanol groups are endcapped by the unique silylation method (except the PREPSIL). Length (mm) 0 Shimpack PREPODS Shimpack PREPC Shimpack PREPCN Shimpack PREPSIL Guard Length (mm) Shimpack PREPODS Shimpack PREPC Shimpack PREPCN Shimpack PREPSIL Shimpack G Series Shimpack G series is also available in preparative columns. (For information of analytical columns, please refer to page.) Length (mm) Shimpack GIST C Guard Shimpack GISS C Guard Shimpack GIST C Guard Shimpack GIST Phenyl Guard Shimpack GIST NH Guard Shimpack HPLC Guidebook High Performance Liquid Chromatograph s

56 Preparative s Shimpack G Series Length (mm) Shimpack GIS C Guard Length (mm) Shimpack GIS C Guard Length (mm) Shimpack GIS CN Guard Length (mm) Shimpack GIS CN Guard

57 Fittings UHPLC Fittings (Pressure Tolerance: 0MPa) A UHPLC fitting is installed to the column inlet tubing in a UHPLC system. Not only can it tolerate pressure of 0MPa, it can be reused when following the proper tightening torque specifications. P/N Description Pack UHPLC Fitting S UHPLC Fitting L* UHPLC Fitting S Extender Tool for UHPLC Fitting * UHPLC Fitting L is mm longer than UHPLC Fitting S. It is recommended for use when a channel switching valve (FCVAH/4AH/6AH) is installed Male Nut Fitting Set (Pressure Tolerance: MPa) The male nut fitting set is assembled with two SUS handtightened male nuts and two PEEK ferrules with tapershaped ends. This product can be tightened by hand to connect with columns that tolerate pressure of MPa. P/N Description Pack Male Nut Fitting Set PEEK Ferrule Male Nut PEEK (Pressure Tolerance: MPa) This nut is superior in chemical compatibility and mechanical strength. It is available for.6 mmi.d. pipe such as stainless, titanium, teflon, tefzel, PEEK etc. Maximum pressure is MPa. Some Shimpack columns are packed with two piece of PEEK male nuts. Extra nuts can be ordered by referring to the part number on the right. P/N Description Pack Male Nut, PEEK Shimpack HPLC Guidebook High Performance Liquid Chromatograph s 7

58 Mobile Phase Cleaner for UHPLC/HPLC Ghost Trap DS/DSHP A new highpressure model for the elimination of impurities from organic solvents has been added to the Ghost Trap DS* lineup. The Ghost Trap DS was codeveloped with Daiichi Sankyo Co., Ltd. It has been designed to effectively adsorb impurities in the mobile phase in order to reduce the time required for method development and impurity analysis. The 0 MPa pressure tolerance of the Ghost Trap DSHP allows it to be used with UHPLC systems. This permits the effective use of the Ghost Trap DS for method development by UHPLC and subsequent transfer to conventional analysis. * DS: Abbreviation of Daiichi Sankyo (D) and Shimadzu (S) Consistently Traps Impurities, Even in Organic Solvents A major feature of the Ghost Trap DS is the ability to remove impurities, even in organic solvents. When the Ghost Trap DS is installed between the gradient mixer and autosampler in reversed phase gradient analysis, it can trap impurities from the piping and gradient mixer in addition to those from the mobile phase. The example on the right shows that it can effectively trap impurities in mobile phase when the Ghost Trap DS is installed just downstream of the gradient mixer. When the Ghost Trap DS is not used When the Ghost Trap DS is used These gradient ghost peaks are completely removed! Example of removing ghost peaks by Ghost Trap DS (The data is provided by Daiichi Sankyo Co., Ltd.) : ODS column Mobile Phase : A) mmol/l Phosphate (Potassium) buffer solution (ph 4.0) /Acetonitrile = 9/ B) Water/Acetonitrile = /9 Flow Rate : 0.6 ml/min Col. Temp : 4 ºC Detection : UV nm Product Information Item P/N Description Dimensions Internal Volume * Pressure Tolerance Ghost Trap DS Cartridge (pcs) Cartridge (pcs) + Holder Cartridge (pcs) Cartridge (pcs) + Holder 0 mml. 7.6 mmi.d. 4.0 mmi.d. mml. Approx. 700 µl Approx. 0 µl MPa Ghost Trap DSHP 8999 Packed type. mmi.d. 0 mml. Approx. 60 µl 0 MPa * Note that a delay volume equivalent to the internal volume of the product occurs if the product is installed downstream of the gradient mixer or the confluence of two pumps. * The product service life differs according to analysis conditions, such as the mobile phase used. * In analysis using an ionpairing reagent, the ionpairing reagent may be retained in the product, influencing the retention time and peak shape. * Before connecting the analytical column, be sure to thoroughly clean the flow path with mobile phase (close to the final concentration for gradient analysis). * Note that some impurities may not be removed. * When performing highpressure analysis exceeding MPa with a UHPLC system, connect the gradient mixer to the Ghost Trap DSHP with a pipe for UHPLC (e.g.8797). 8