Shim-pack GIST/GISS/GIS/GWS Series
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- Alan Thompson
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1 and Normal Phase HPLC Shim-pack GIST/GISS/GIS/GWS Series C0-EB
2 Shimadzu Liquid Chromatographs Maximizing the Potential of UHPLC/HPLC Analysis Meet the Nexera X, the most advanced UHPLC available today. The flexible system design achieves a true fusion between UHPLC and HPLC technologies, enabling the Nexera X to be used for a much broader range of applications. This completely new UHPLC system not only offers maximum speed, sensitivity, resolution, stability, and reliability, it also features the revolutionary i-pdea* separation technology and an i-drec** function that extends the dynamic range so that both concentrated and trace components can be quantitated simultaneously. * Intelligent Peak Deconvolution Analysis (patent pending) ** Intelligent Dynamic Range Extension Calculator (patent pending) The Most Accessible UHPLC Available Developed with expandability and compatibility in mind, the Nexera XR ultra high performance liquid chromatograph enables more customers to make use of high-speed, high-resolution systems. Configure the optimal system to meet the specific analysis objective by selecting from among a wide range of highly accurate and reliable modules. The next milestone in the evolution of liquid chromatography, the Nexera XR promises to become an indispensable tool in laboratories in a variety of fields, including pharmaceuticals, biochemistry, chemistry, environmental, and foods. Specifications P. Guide for Selection of P. 6 Contents Shim-pack GIST C P. Shim-pack GISS C P. Shim-pack GIS C P.
3 HPLC systems are currently used in a wide variety of fields. Reliable analytical data and a more efficient total analysis workflow are required to achieve faster development of new drugs, ensure food safety, and meet higher standards in environmental regulations. Many analysis techniques use LCMS, which requires a front-end HPLC system to provide solvent delivery performance in the micro to semi-micro range and injectors with low sample carryover. Prominence is a network-ready HPLC system that meets the demands of today s advanced users. Prominence features the world s first Web control, fastest sample injection, and highest detection sensitivity performance functions that surpass current HPLC technology. New Industry Standard HPLCs innovative Realization of Advanced Laboratory Advanced functions free operators from the laboratory Remote monitoring using a smart device Excellent baseline stability unaffected by circumstances Ultrafast injection cycle reduces analysis times Ultra-low carryover Use of multiple detectors expands application range intuitive Achieving Easier Operation Unified graphical user interface between system and workstation Simplified batch file creation Displays chromatograms in real time intelligent Smart Features Increase Work Efficiency Fully automated analysis: reliably perform analyses according to prescribed procedures Migrate existing methods from either Shimadzu or non-shimadzu systems Shim-pack GWS C P. 7 Applications P. 6 Shim-pack GIST C8 P. Shim-pack GIST Phenyl P. Index P. Normal Phase Fittings P. 8 Shim-pack GIST NH P. Shim-pack GIS CN P. Mobile Phase Cleaner for HPLC P. 9
4 Specifications Shim-pack GIS Series High retentivity, lower column back pressure, high inertness Shim-pack GIS series columns are packed with high purity silica gel and are your ideal generic HPLC columns. Highly uniform particles ensure stable mobile phase delivery and outstanding low pressure. The Shim-pack GIS series not only reduces your cost of solvents, but relieves your system load as well. A uniform silica surface and stable chemical modification ensure high analysis reproducibility. Shim-pack GIST Series Ultra-high inertness and high durability Shim-pack GIST series columns are packed with newly developed high-purity porous spherical silica. Superior inertness not only improves analytical precision, but also increases column durability. Shim-pack GIST series is capable of tolerating a wider ph (-) situation, making it suitable for analysis of many different compounds. C, C8, Phenyl and NH columns are offered to cover an extended range of selectivity and meet diverse research and development needs. Furthermore, Shim-pack GIST series is designed with various particle size ranges, which make it easy to transfer your analysis from ultra-high-speed to conventional methods. Bonded Phase Analysis Mode Features USP Code Shim-pack GIST C Octadecyl Groups Ultra-high inertness and high durability L,, Shim-pack GISS C Octadecyl Groups High-speed analysis with ultra-high inertness and high durability L.9,, Shim-pack GIS C Octadecyl Groups High retentivity, lower column back pressure, high inertness L,,,, Shim-pack GWS C Octadecyl Groups Economical C column with medium retentivity and high inertness L Shim-pack GIST C8 Octyl Group Ultra-high inertness and high durability L7,, Shim-pack GIST Phenyl Phenyl Group Extremely strong π-π interactions L,, Shim-pack GIST NH Aminopropyl Group HILIC mode, Normal Phase Sugar analysis L8, Shim-pack GIS CN Cyanopropryl Group, Normal Phase Suitable in either reversed phase or normal phase mode L,
5 Specifications Shim-pack GISS Series High-speed analysis with ultra-high inertness and high durability Shim-pack GISS series maintains the same ultra-high inertness and wider ph range as the Shim-pack GIST series while providing rapid separations with symmetric peaks. The optimization of surface area, pore size and chemical bonding delivers superior peak shapes, making these columns ideal for LC/MS/MS Specifications analysis. Shim-pack GISS series also includes the.9μm and μm HP series for UHPLC analyses. Shim-pack GWS Series Guide for Selection of Medium retentivity and high inertness Shim-pack GWS series is an economical generic C column designed with high cost performance. A uniform pore size ensures low pressure. Complete end-capping makes it possible to be used for analysis of acidic or basic compounds. Shim-pack GWS series is the choice for cost control. Pore Size (nm) Surface Area (m /g) Carbon Loading (%) End Cap Inertness ph Range Page # Normal Phase Shim-pack GIST C - Shim-pack GISS C Shim-pack GIS C Shim-pack GWS C Shim-pack GIST C8 Shim-pack GIST Phenyl Shim-pack GIST NH Applications Index Shim-pack GIS CN -7. Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
6 Guide for Selection of Selection of Separation Mode by Physical Property Solubility Type of Compound Separation Mode Dissolve easily in water Polar and acidic Reversed phase Not dissolve easily Polar and HILIC mode in ethanol basic (for higher retention) Polar and neutral Normal phase Dissolve easily Mid-polar and in ethanol Not dissolve easily neutral Low-polar and Cation exchange Compounds in acidic water Not dissolve easily in basic water Dissolve easily acidic salts Low-polar and basic salts Low-polar and Anion exchange Ion exclusion in acidic water basic Dissolve easily Low-polar and in basic water acidic Dissolve easily Low-polar and in ethanol neutral Not dissolve easily in ethanol Polymer Size exclusion Not dissolve easily in water Selection by Component Component Separation Mode Organic acids Common organic acids Reversed phase Shim-pack GISS C Shim-pack GIS C Shim-pack GWS C Oxalic acid HILIC mode Shim-pack GIST NH 6
7 Guide for Selection of Component Separation Mode Vitamins Mixture of water soluble / oil soluble vitamins Reversed phase Shim-pack GIST C Shim-pack GIS C Shim-pack GWS C Specifications Vitamin C HILIC mode Direct analysis Shim-pack GIST NH Oil soluble vitamins Vitamin D Vitamin E Vitamin A Vitamin K Normal phase Reversed phase Shim-pack GIST NH Shim-pack GIST C Guide for Selection of Water soluble vitamins Detector Oil soluble vitamin Detector Vitamin B (thiamin) Vitamin B (riboflavin) Vitamin B6 (pyridoxine) C C C UV (70 nm) RF (Post-column derivatization) UV (70 nm) RF (Post-column derivatization) UV (90 nm) Vitamin A (retinol) Vitamin D Vitamin E (tocopherol) C C NH C UV ( nm) RF (Ex. 0 nm, Em. 60 nm) UV (6 nm) UV (9 nm) RF (Ex. 9 nm, Em. nm) Vitamin C (ascorbic acid) NH UV ( nm) Vitamin K C UV ( nm) RF (Post-column derivatization) Niacin C UV Vitamin M (folic acid) Pantothenic acid C C UV (80 nm) UV Normal Phase Shim-pack SCR-N* Sugars Monosaccharides Ligand exchange Shim-pack SCR-C* Mono- ~ trisaccharides Oligosaccharides Derivatized sugars HILIC mode Anion exchange HILIC mode Reversed phase Shim-pack SCR-P* Shim-pack GIST NH Shim-pack ISA-07* Shim-pack GIST NH Shim-pack GIST C Shim-pack GISS C Shim-pack GIS C Applications Index Shim-pack GWS C *For more details, please refer to individual catalog. Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC 7
8 Guide for Selection of Component Separation Mode Nucleic acid bases Nucleic acids Cation exchange Shim-pack WCX-* and nucleosides Ion pair + Reversed phase Shim-pack GIST C Shim-pack GISS C Shim-pack GIS C Shim-pack GWS C HILIC mode Shim-pack GIST NH Nucleotides Anion exchange Shim-pack WAX-* DNA and RAN Gel filtration Shim-pack Diol* Anion exchange Shim-pack WAX-* Shim-pack PA-DEAE* Shim-pack PA-QA* Amino acids Free amino acids Protein-hydrolyzed amino acids Cation exchange Cation exchange Reserved phase Ion pair + Reversed phase Shim-pack Amino Li* Shim-pack Amino Na* Shim-pack GIST C Shim-pack GIS C Shim-pack GWS C Shim-pack HRC-ODS* Shim-pack GIST C Post-column derivatization Fluorescing with OPA Pre-column derivatization Pre-column derivatization Fluorescing with OPA HILIC mode Shim-pack GIST NH 8
9 Guide for Selection of Component Separation Mode Peptides, Proteins Peptides Reversed phase Shim-pack GIST C Shim-pack GISS C Shim-pack GIS C Specifications Shim-pack GWS C Glycopeptides HILIC mode Shim-pack GIST NH Gel filtration Cation exchange Shim-pack Diol* Shim-pack WCX-* Shim-pack PA-CM* Shim-pack PA-SP* Guide for Selection of Anion exchange Shim-pack PA-DEAE* Shim-pack PA-QA* Reversed phase Shim-pack CLC-ODS* Shim-pack HRC-ODS* Pesticides, Medicines Veterinary Reversed phase Shim-pack GIST C medicines Shim-pack GIST Phenyl Shim-pack GISS C Shim-pack GIS C Shim-pack GWS C For higher retention For weaker retention Normal Phase HILIC mode Shim-pack GIST NH DNPH aldehydes Reversed phase Shim-pack GIST C Applications Index *For more details, please refer to individual catalog. Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC 9
10 Shim-pack GIST C Ultra High Inertness, High Durability Shim-pack GIST C has superior inertness, which improves analysis precision and increases column durability. In addition, it can be used to analyze strong ionic compounds and difficult to absorb samples, which helps to obtain symmetrical peaks and high reproducibility. Furthermore, because of the new silica gel, Shim-pack GIST C is suitable for wider ph (-) analysis at a consistent performance. This enables use of a silica-based column under alkaline conditions. Bonded Phase Pore Size Surface Area Carbon Loading End Cap ph Range USP Code Octadecyl Groups μm, μm, μm nm m /g % Complete to L Analysis Examples *For more application data, please refer to page 6. Nine Pesticides on a Golf Course Peaks ( mg/l each). Asulam. Oxine Copper. Triclopyr. Mecoprop (MCPP). Thiram 6. Flazasulfuron 7. Halosulfuron Methyl 8. Siduron 9. Azoxystrobin Nucleoside and Nucleic acid base Peaks ( mg/l each). Cytosine. Uracil. Guanine. Adenine. Cytidine 6. Uridine 7. Thymine 8. Adenosine 9. Guanosine. Thymidine : Shim-pack GIST-HP C (0 mml..0 mmi.d., μm) : A) mm KH PO (ph., H PO ) B) CH CN A/B = 60/0 - min - 0/60 Flow Rate :. ml/min Detection : UV nm Injection Vol. :.0 μl : Shim-pack GIST C (0 mml..6 mmi.d., μm) : 0. M NH PO, 0. M NaClO (ph.0, H PO ) Flow Rate :.0 ml/min Detection : UV 60 nm Injection Vol. : μl Analytical
11 Shim-pack GIST C Cartridge Guard Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Analytical (High-Pressure Series) *For UHPLC fittings, please refer to page Pressure Tolerance (MPa) 80 Specifications Guide for Selection of Cartridge Guard (High-Pressure Series) Preparative Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Guard for Preparative Pressure Tolerance (MPa) 80 Pressure Tolerance (MPa) Normal Phase Applications Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
12 Shim-pack GISS C Ideal for Rapid Analysis Shim-pack GISS C maintains the same ultra-high inertness and wider ph range as the Shim-pack GIST C, and provides rapid separations with symmetrical peaks. The optimization of surface area, pore size and chemical bonding delivers superior peak shapes. It is ideal for LC/MS/MS analysis and enables MS-compatible buffers to be used due to extremely inert silica gel. Bonded Phase Pore Size Surface Area Carbon Loading End Cap ph Range USP Code Octadecyl Groups.9 μm, μm, μm nm 0 m /g 9 % Complete to L Analysis Example *For more application data, please refer to page 6. β-blocker Peaks (0 μg/l each) Q > Q. Acebutolol : 7. > 6.0 (+). Atenolol : 67. >.00 (+). Labetalol : 9.00 >.9 (+). Nadolol :.0 >.00 (+). Pindolol : 9.80 > 6.00 (+) min min Flow Rate : Shim-pack GISS C ( mml. mmi.d.,.9 μm) : A) mm Ammonium Formate in H O B) mm Ammonium Formate in CH OH A/B = 70/0-0. min - 0/60-0. min - 0/0-0. min - 0/0-0.0 min - 70/0-0. min - 70/0, v/v : 0.6 ml/min Detection : LC/MS/MS (ESI, Positive, Negative MRM) Analytical
13 Shim-pack GISS C Cartridge Guard Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Analytical (High-Pressure Series) *For UHPLC fittings, please refer to page Pressure Tolerance (MPa) 80 Specifications Guide for Selection of Cartridge Guard (High-Pressure Series) Cartridge Guard (pcs)...0 Pressure Tolerance (MPa) Cartridge Guard (pcs) and Holder Pressure Tolerance (MPa) Normal Phase Preparative Guard for Preparative Applications Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
14 Shim-pack GIS C High Retentivity, Lower Back Pressure Widely used octadecyl bonded silica gel enables the Shim-pack GIS C to have strong hydrophobic interaction and low absorption of ionic compounds. In addition, highly uniform particles ensure stable mobile phase delivery and outstanding low pressure. Shim-pack GIS C is ideal for preparative separations. Higher surface area silica and strong retentivity provide high preparative loading capacity without sacrificing peak shape. Bonded Phase Pore Size Surface Area Carbon Loading End Cap ph Range USP Code Octadecyl Groups μm, μm, μm, μm, μm nm m /g % Complete to 7. L Analysis Example *For more application data, please refer to page 6. Food Dyes Peaks. Tartrazine. Amaranth. Ingigocarmine. New coccine. Sunset Yellow FCF 6. Naphthol Yellow S 7. Uranine 8. Allura Red AC 9. Ponceau R 7.6 mg/l.8 mg/l 7.6 mg/l.8 mg/l. mg/l 7.6 mg/l.8 mg/l. mg/l 7.6 mg/l. Ponceau SX. Orange I. Fast green FCF. Brilliant blue FCF. Ponceau R. Erythrosine. Azure Blue VX 7. Orange II. Acid red : Shim-pack GIS C (0 mml..6 mmi.d, μm) : A) mm Na HPO (ph6.9 ; mm NaH PO ) B) CH CN A/B = 90/ - min - 6/ Flow Rate :.0 ml/min Detection : UV 70 nm Injection Vol. : μl. mg/l. mg/l.0 mg/l.0 mg/l 7.6 mg/l. mg/l.0 mg/l 7.6 mg/l.0 mg/l Analytical
15 Shim-pack GIS C Analytical 0 0 Cartridge Guard Analytical (High-Pressure Series) Cartridge Guard (High-Pressure Series) Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder *For UHPLC fittings, please refer to page Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Pressure Tolerance (MPa) 80 Pressure Tolerance (MPa) 80 Pressure Tolerance (MPa) 80 Specifications Guide for Selection of Normal Phase Applications Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
16 Shim-pack GIS C Preparative Guard for Preparative
17 Shim-pack GWS C Economical Choice Shim-pack GWS C is packed with high-purity silica gel. A uniform pore size ensures low pressure, while complete end-capping makes it possible for analysis of acidic or basic compounds. Shim-pack GWS series is an ideal choice for cost control. Bonded Phase Pore Size Surface Area Carbon Loading End Cap Octadecyl Groups μm nm m /g 9. % Complete Specifications ph Range to 7. USP Code L Analysis Examples *For more application data, please refer to page 6. Cold medication Peaks ( mg/l each). Potassium GuaiacoI sulfonate. Acetaminophen. Caffeine. Ethenzamide. Allyl isopropyi acetyi urea 6. Isopropylantipyrine 7. Ibuprofen Puerariae radix Peaks. Puerarin Guide for Selection of : Shim-pack GWS C (0 mml..6 mmi.d., μm) : A) 0.%H PO B) CH CN A/B = 90/ - min - 90/ - min - 0/0 Flow Rate :.0 ml/min. Detection : UV nm Injection Vol. : μl : Shim-pack GWS C ( mml..6 mmi.d., μm) : A) H O B) CH CN A/B = 89/ Flow Rate :.0 ml/min. Col. Temp. : ºC Detection : UV nm Injection Vol. : μl Normal Phase Analytical Cartridge Guard Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Applications Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC 7
18 Shim-pack GIST C8 Ultra-High Inertness, High Durability Shim-pack GIST C8 is packed with high-purity porous spherical silica for delivering the same extreme inertness to elute either basic or acidic compounds without undesired adsorption. Low retentivity and no sample adsorption enable analysis of natural samples. Shim-pack GIST C8 is the ideal choice for the rapid analysis of hydrophobic compounds. Bonded Phase Pore Size Surface Area Carbon Loading End Cap ph Range USP Code Octyl Group μm, μm, μm nm m /g 8 % Complete to L7 Analysis Examples *For more application data, please refer to page 6. Pyridine derivatives Peaks ( mg/l each). Pyridine. α-picoline. γ-picoline. β-picoline.,6-lutidine 6.,-Lutidine 7.,-Lutidine 6 7 Phospholipids Peaks. Phosphatidyl-L-Serine (PS) μg/ml Q: 788. Q: 8.0. L-α-Phosphatidyl-DL-glycerol, Distearoyl (PG) 0. μg/ml Q: 777. Q: 8.. L-α-Phosphatidylcholine (PC) (0.0 μg/ml) 0.0 μg/ml Q: 806. Q: 8.. L-α-Phosphatidylethanolamine, Distearoyl (PE) 0. μg/ml Q: 76.0 Q: 8. (in CHCl /CH OH = /, v/v) min : Shim-pack GIST C8 (0 mml..6 mmi.d., μm) : A) mm Na HPO (ph 8.0, H PO ) B) THF A/B = 87/, v/v Flow Rate :.0 ml/min Detection : UV 60 nm Injection Vol. : μl : Shim-pack GIST C8 (0 mml.. mmi.d., μm) : 0. % HCOOH, mm HCOONH in CH OH Flow Rate : 0. ml/min Detection : LC/MS/MS (ESI, Negative, MRM) Injection Vol. : μl Analytical
19 Shim-pack GIST C8 Cartridge Guard Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Analytical (High-Pressure Series) *For UHPLC fittings, please refer to page Pressure Tolerance (MPa) 80 Specifications Guide for Selection of Cartridge Guard (High Pressure series) Preparative Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Guard for Preparative Pressure Tolerance (MPa) 80 Pressure Tolerance (MPa) Normal Phase Applications Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
20 Shim-pack GIST Phenyl Extremely Strong π-π Interactions The extremely unique phase characteristics of Shim-pack GIST Phenyl are critical to resolving compounds that could not be separated with a C or C8 phase column. In addition to π-π interactions, Shim-pack GIS Phenyl provides hydrogen bonding secondary interactions, which results in retaining polar compounds at the same time. As the phenyl groups are directly bonded to the silica gel, Shim-pack GIS Phenyl is also capable of the analysis of structural isomers due to its high stereo-selectivity. Bonded Phase Pore Size Surface Area Carbon Loading End Cap ph Range USP Code Phenyl Group μm, μm, μm nm m /g % None to 7. L Analysis Example *For more application data, please refer to page 6. Steroidal anti-inflammatory drug Peaks (0. mg/l each). Hydrocortisone. Prednisolone : Shim-pack GIST Phenyl ( mml.. mmi.d., μm) : A) H O/HCOOH = 0/0.0, v/v B) CH OH/HCOOH = 0/0.0, v/v A/B = 60/0, v/v Flow Rate : 0.6 ml/min Detection : LC/MS/MS (ESI, Positive, MRM) Injection Vol. : μl Analytical
21 Shim-pack GIST Phenyl Cartridge Guard Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Analytical (High-Pressure Series) *For UHPLC fittings, please refer to page Pressure Tolerance (MPa) 80 Specifications Guide for Selection of Cartridge Guard (High Pressure series) Preparative Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Guard for Preparative Pressure Tolerance (MPa) 80 Pressure Tolerance (MPa) Normal Phase Applications Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
22 Shim-pack GIST NH Suitable for Sugar Analysis New high-purity porous spherical silica chemically bonded with the aminopropyl group ensures the superior stability of Shim-pack GIST NH. It is capable of the analysis of vitamin E or simultaneous analysis of sugars that are hard to separate in reversed phase mode. In addition, due to being primarily amine-bound, Shim-pack GIST NH can analyze sugars with no separation of anomers, even under low temperature conditions. Furthermore, Shim-pack GIST NH delivers highly reliable reproducibility and stability with accurate qualitative and quantitative results. Bonded Phase Pore Size Surface Area Carbon Loading End Cap ph Range USP Code Aminopropyl Group μm, μm nm m /g 7 % None to 7. L8 Analysis Examples *For more application data, please refer to page 6. Sugars Peaks. Fructose. Glucose. Sucrose. Maltose : Shim-pack GIST NH ( mml..6 mmi.d., μm) : A) H O B) CH CN A/B = /7, v/v Flow Rate :.0 ml/min Detection : RID Injection Vol. : μl Vitamin E Peaks ( mg/l each). α-tocopherol. β-tocopherol. γ-tocopherol. δ-tocopherol : Shim-pack GIST NH ( mml..6 mmi.d., μm) : A) n-hexane B) Ethyl acetate A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 ºC Detection : UV 90 nm Injection Vol. : μl
23 Shim-pack GIST NH Analytical Specifications Guide for Selection of Cartridge Guard Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Preparative Normal Phase Guard for Preparative Applications Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
24 Shim-pack GIS CN Suitable in Either or Normal Phase Mode Shim-pack GIS CN is capable of either normal phase or reversed phase analysis. Cyanopropryl groups bonded to silica gel with high density increases the difference recognition of hydrophilicity and the durability. Due to no end-capping, it is capable of analysis utilizing cyano group characteristics. In reversed phase mode, separation can be achieved for those compounds that could not be separated on straight-chain-alkyl columns, such as C or C8 bonded phases. When using the column for reversed phase mode, fully equilibrate the column before use. Bonded Phase Pore Size Surface Area Carbon Loading End Cap ph Range USP Code Cyanopropryl Group μm, μm nm m /g % None to 7. L Analysis Examples *For more application data, please refer to page 6. gamma-oryzanol Free Amino acids and Organic acids Peaks. gamma-oryzanol. vanillin Peaks. Asparagine H O (0.7 mg/ml). Aspartic acid (0.7 mg/ml). Fumaric acid (0.0 mg/ml). Maleic acid (0.0 mg/ml) : Shim-pack GIS CN (0 mml..6 mmi.d, μm) : hexane:-propanol:acetic acid = 9:: Flow Rate :.0 ml/min Col. Temp. : 0 ºC Detection : UV nm Injection Vol. : μl : Shim-pack GIS CN ( mml..6 mmi.d., μm) : mm KH PO (ph.0; H PO ) Flow Rate :.0 ml/min Detection : UV nm Injection Vol. : μl Analytical
25 Shim-pack GIS CN Cartridge Guard Cartridge Guard (pcs) Cartridge Guard (pcs) and Holder Preparative Specifications Guide for Selection of Guard for Preparative Normal Phase Applications Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
26 Applications <Foods> Linear Fatty Acids C C C C Peaks ( mg/ml each). Acetic acid (C) 8. Pelargonic acid (C9). Propionic acid (C) 9. Capric acid (C). Butyric acid (C). Undecanoic acid (C). Valeric acid (C). Lauric acid (C). Caproic acid (C6). Tridecanoic acid (C) 6. Enanthic acid (C7). Myristic acid (C) 7. Caprylic acid (C8). Pentadecanoic acid (C) C6 6 C7 7 C8 8 C9 9 C C C C C C Parabens Peaks ( μg/ml each). -Phenoxyethanol. Methyl p-hydroxybenzoate. Ethyl p-hydroxybenzoate. Isopropyl p-hydroxybenzoate. Propyl p-hydroxybenzoate 6. Isobutyl p-hydroxybenzoate 7. Butyl p-hydroxybenzoate : Shim-pack GIST C (0 mml..6 mmi.d., μm) : A) 0. % H PO in H O B) 0. % H PO in CH CN A/B = 90/ - min - /90 - min - /90, v/v (Mixed by a gradient mixer) Flow Rate :.0 ml/min Detection : UV nm Injection Vol. : μl : Shim-pack GIST-HP C (0 mml..0 mmi.d., μm) : A) H O B) CH OH A/B = /, v/v Flow Rate : 0.8 ml/min Detection : UV 6 nm Injection Vol. : μl Food Dyes Peaks. Tartrazine. Amaranth. Ingigocarmine. New coccine. Sunset Yellow FCF 6. Naphthol Yellow S 7. Uranine 8. Allura Red AC 9. Ponceau R mg/l.8 mg/l 7.6 mg/l.8 mg/l. mg/l 7.6 mg/l.8 mg/l. mg/l 7.6 mg/l 7. Ponceau SX. Orange I. Fast green FCF. Brilliant blue FCF. Ponceau R. Erythrosine. Azure Blue VX 7. Orange II. Acid red. mg/l. mg/l.0 mg/l.0 mg/l 7.6 mg/l. mg/l.0 mg/l 7.6 mg/l.0 mg/l Phospholipids Peaks. Phosphatidyl-L-Serine (PS). L-α-Phosphatidyl-DL-glycerol, Distearoyl (PG). L-α-Phosphatidylcholine (PC). L-α-Phosphatidylethanolamine, Distearoyl (PE) μg/ml 0. μg/ml 0.0 μg/ml 0. μg/ml (in CHCl /CH OH = /, v/v) : Shim-pack GIS C (0 mml..6 mmi.d., μm) : A) mm Na HPO (ph6.9 ; mm NaH PO ) B) CH CN A/B = 90/ - min - 6/ Flow Rate :.0 ml/min Detection : UV 70 nm Injection Vol. : μl min : Shim-pack GIST C8 (0 mml.. mmi.d., μm) : 0. % HCOOH, mm HCOONH in CH OH Flow Rate : 0. ml/min Detection : LC/MS/MS (ESI, Negative, MRM) Injection Vol. : μl <Pharmaceuticals> Acesulfame Potassium Glycyrrhiza Peaks. Saccharin. Acesulfame potassium Peaks. Glycyrrhizic acid 00 mg/l 0 0 : Shim-pack GIST NH ( mml..6 mmi.d., μm) : A) % H PO in H O B) CH CN A/B = 0/60, v/v Flow Rate :.0 ml/min Detection : UV 0 nm Injection Vol. : μl : Shim-pack GIST C (0 mml..6 mmi.d., μm) : A).% CH COOH in H O B) CH CN A/B = 60/0, v/v Flow Rate : 0. ml/min Col. Temp. : ºC Detection : UV nm Injection Vol. : μl 6
27 Applications Tetracyclines Olmesartan Medoxomil Peaks ( mg/l). Oxytetracycline. Tetracycline. Chlortetracycline Peaks. Olmesartan medoxomil. p-hydroxybenzoic acid isobutyl ester mg/l mg/l Specifications 0 0 : Shim-pack GIST C (0 mml..6 mmi.d., μm) : A) Imidazole buffer B) CH OH A/B = 80/, v/v (Premix) Flow Rate :.0 ml/min Detection : RF Ex 80 nm Em nm Injection Vol. : μl : Shim-pack GIST C (0 mml..6 mmi.d., μm) : A) mm KH PO (ph., H PO ) B) CH CN A/B = 66/, v/v Flow Rate :.0 ml/min Detection : UV nm Injection Vol. : μl Guide for Selection of 7 Anti-depressant Drugs Peaks (0 ng/ml each). Sulpiride. Milnacipran. Trazodone. Mianserin. Amoxapine 6. Doxepin 7. Paroxetine 8. Desipramine 9. Fluvoxamine 7. Imipramine. Nortriptyline. Maprotiline. Amitriptyline. Trimipramine. Fluoxetine. Sertraline 7. Clomipramine β-blocker Peaks (0 μg/l each). Acebutolol. Atenolol. Labetalol. Nadolol. Pindolol min : Shim-pack GIST-HP C (0 mml.. mmi.d., μm) : A) 0. % HCOOH in H O B) 0. % HCOOH in CH CN A/B = 98/ - 0. min - 60/0 -. min - 60/0, v/v Flow Rate : 0. ml/min Detection : LC/MS/MS (ESI, Negative, MRM) Injection Vol. : μl Flow Rate : Shim-pack GISS C ( mml.. mmi.d.,.9 μm) : A) mm Ammonium Formate in H O B) mm Ammonium Formate in CH OH A/B = 70/0-0. min - 0/60-0. min - 0/0-0. min - 0/0-0.0 min - 70/0-0. min - 70/0, v/v : 0.6 ml/min Detection : LC/MS/MS (ESI, Positive, Negative MRM) Normal Phase Cold Medication Peaks ( mg/l each). Potassium GuaiacoI sulfonate. Acetaminophen. Caffeine. Ethenzamide. Allyl isopropyi acetyi urea 6. Isopropylantipyrine 7. Ibuprofen : Shim-pack GWS C (0 mml..6 mmi.d., μm) : A) 0.%H PO B) CH CN A/B = 90/ - min - 90/ - min - 0/0 Flow Rate :.0 ml/min. Detection : UV nm Injection Vol. : μl 6 7 Puerariae Radix Peaks. Puerarin : Shim-pack GWS C ( mml..6 mmi.d, μm) : A) H O B) CH CN A/B = 89/ Flow Rate :.0 ml/min. Col. Temp. : ºC Detection : UV nm Injection Vol. : μl Applications Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC 7
28 Applications <Biochemicals> Steroidal Anti-inflammatory Drug Peaks (0. mg/l each). Hydrocortisone. Prednisolone Nucleoside and Nucleic Acid Base Peaks ( mg/l each). Cytosine. Uracil. Guanine. Adenine. Cytidine 6. Uridine 7. Thymine 8. Adenosine 9. Guanosine. Thymidine : Shim-pack GIST Phenyl ( mml.. mmi.d., μm) : A) H O/HCOOH = 0/0.0, v/v B) CH OH/HCOOH = 0/0.0, v/v A/B = 60/0, v/v Flow Rate : 0.6 ml/min Detection : LC/MS/MS (ESI, Positive, MRM) Injection Vol. : μl <Pesticides> : Shim-pack GIST C (0 mml..6 mmi.d., μm) : 0. M NH PO, 0. M NaClO (ph.0, H PO ) Flow Rate :.0 ml/min Detection : UV 60 nm Injection Vol. : μl Tap Water Pesticides Nine Pesticides on a Golf Course 9 Positive Negative Peaks ( mg/l each). Asulam. Oxine Copper. Triclopyr. Mecoprop (MCPP). Thiram 6. Flazasulfuron 7. Halosulfuron Methyl 8. Siduron 9. Azoxystrobin : Shim-pack GIST C (7 mml.. mmi.d., μm) : A) mm Ammonium Acetate B) mm Ammonium Acetate in CH OH A/B = 9/ - min - 60/0 - min - /7 - min - 0/0-6 min - 0/0 - ( min), v/v Flow Rate : 0. ml/min Detection : LC/MS/MS (ESI, MRM) Injection Vol. : 0 ml : Shim-pack GIST-HP C (0 mml..0 mmi.d., μm) : A) mm KH PO (ph., H PO ) B) CH CN A/B = 60/0 - min - 0/60 Flow Rate :. ml/min Detection : UV nm Injection Vol. :.0 μl Pesticides Diquat and Paraquat Peaks ( μg/l). Paclobutrazole. Diniconazole. Propiconazole. Difenoconazole Peaks. Diquat. Paraquat : Shim-pack GIST Phenyl (0 mml.. mmi.d., μm) : A) 0. % HCOOH in H O B) 0. % HCOOH in CH CN A/B = 60/0 - min- 0/ min- 60/0 - min- 60/0, v/v Flow Rate : 0. ml/min Detection : LC/MS/MS (ESI, Positive, MRM) Injection Vol. : μl : Shim-pack GIST C ( mml.. mmi.d., μm) : A) H O, 0. % IPCC MS- B) CH CN, 0. % IPCC MS- A/B = 9/7, v/v Flow Rate : 0. ml/min Detection : LC/MS/MS (ESI, Positive, MRM) Injection Vol. : μl 8
29 Applications <Environmental> <Others> Kinds of Aldehydes Peaks (0 μg/l). DNPH-Formaldehyde. DNPH-Acetaldehyde. DNPH-Acetone. DNPH-Acrolein. DNPH-Propionaldehyde 6. DNPH-Crotonaldehyde 7. DNPH-Methylethylketone 8. DNPH-Methacrolein 9. DNPH-n-Butyraldehyde. DNPH-Benzaldehyde. DNPH-n-Valeraldehyde. DNPH-m-Tolualdehyde. DNPH-Hexanal Pre-column Derivatized Aflatoxins Peaks ( ng/ml each). Aflatoxin G. Aflatoxin B. Aflatoxin G. Aflatoxin B Specifications : Shim-pack GIST-HP C (0 mml..0 mmi.d., μm) : A) H O B) CH CN C) THF A/B/C = //, v/v/v Flow Rate :. ml/min Detection : UV 60 nm Injection Vol. : μl : Shim-pack GIST C (0 mml..6 mmi.d., μm) : A) H O B) CH OH : C) CH CN : A/B/C = 60/0/, v/v/v (Premix) Flow Rate :.0 ml/min Detection : RF Ex. 6 nm, Em. nm Injection Vol. : μl Guide for Selection of Water-soluble Vitamins Peaks. Ascorbic acid. Nicotinic acid. Nicotinamide. Pantothenic acid. Pyridoxal 0 mg/l mg/l mg/l 0 mg/l mg/l Pyridoxine mg/l 7. Pyridoxamine mg/l 8. Thiamine mg/l 9. Folic acid mg/l. Riboflavin mg/l 8 9 Vitamin K Peaks ( mg/l each). Vitamin K (MK-). Vitamin K. Vitamin K (MK-7) : Shim-pack GIST C (0 mml..0 mmi.d., μm) : A) mm KH PO (ph., H PO ) + mm IPCC-06 B) CH CN A/B = 98/ - 8 min - 88/ - min - 88/, v/v Flow Rate : 0.6 ml/min Col. Temp. : 0 ºC Detection : UV nm Injection Vol. : μl : Shim-pack GIST C8 (0 mml..0 mmi.d., μm) : CH CN Flow Rate :.0 ml/min Detection : UV 70 nm Injection Vol. : μl Normal Phase Vitamin E Peaks ( mg/l each). α-tocopherol. β-tocopherol. γ-tocopherol. δ-tocopherol : Shim-pack GIST NH ( mml..6 mmi.d., μm) : A) n-hexane B) Ethyl acetate A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 ºC Detection : UV 90 nm Injection Vol. : μl Amino Acids Peaks (0 μg/ml). Aspartic acid. Glutamine. Threonine. Proline : Shim-pack GISS C ( mml..6 mmi.d., μm) : A) mm IPCC-08 in (0.% H PO in H O) (IPCC-08: Sodium -Octanesulfonate) B) CH CN A/B = 9/, v/v Flow Rate :.0 ml/min Detection : UV nm Injection Vol. : μl Applications Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC 9
30 Applications Free Amino Acids and Organic Acids Peaks. Asparagine H O (0.7 mg/ml). Aspartic acid (0.7 mg/ml). Fumaric acid (0.0 mg/ml). Maleic acid (0.0 mg/ml) Pyridine Derivatives Peaks ( mg/l each). Pyridine. α-picoline. γ-picoline. β-picoline.,6-lutidine 6.,-Lutidine 7.,-Lutidine : Shim-pack GIS CN ( mml..6 mmi.d., μm) : mm KH PO (ph.0; H PO ) Flow Rate :.0 ml/min Detection : UV nm Injection Vol. : μl : Shim-pack GIST C8 (0 mml..6 mmi.d., μm) : A) mm Na HPO (ph 8.0, H PO ) B) THF A/B = 87/, v/v Flow Rate :.0 ml/min Detection : UV 60 nm Injection Vol. : μl Pyridine Peaks. Uracil. Pyridine. Phenol Sugars Peaks. Fructose. Glucose. Sucrose. Maltose Flow Rate Detection : UV nm : Shim-pack GIS CN (0 mml..6 mmi.d., μm) : A) H O B) CH OH A/B = 70/0, v/v :.0 ml/min : Shim-pack GIST NH ( mml..6 mmi.d., μm) : A) H O B) CH CN A/B = /7, v/v Flow Rate :.0 ml/min Detection : RID Injection Vol. : μl Malto Oligosugars gamma-oryzanol Peaks (. mg/ml). Maltose. Maltotriose. Maltotetraose. Maltopentaose. Maltohexaose 6. Maltoheptaose 6 Peaks. gamma-oryzanol. vanillin : Shim-pack GIST NH ( mml..6 mmi.d., μm) : A) H O B) CH CN A/B = /6, v/v (Premix) Flow Rate :.0 ml/min Col. Temp. : 0 ºC Detection : RF Injection Vol. : μl : Shim-pack GIS CN (0 mml..6 mmi.d., μm) : hexane:-propanol:acetic acid = 9:: Flow Rate :.0 ml/min Col. Temp. : 0 ºC Detection : UV nm Injection Vol. : μl 0
31 Specifications Guide for Selection of Normal Phase Applications Index Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
32 Index
33 Specifications Guide for Selection of Normal Phase Applications Index Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
34 Index
35 Specifications Guide for Selection of Normal Phase Applications Index Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
36 6 Index
37 7 Specifications Guide for Selection of Normal Phase Applications Index Index Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC
38 Fittings UHPLC Fittings (Pressure Tolerance: 0MPa) A UHPLC fitting is installed to the column inlet tubing in a UHPLC system. Not only can it tolerate pressure of 0MPa, it can be re-used when following the proper tightening torque specifications. P/N Description Pack UHPLC Fitting S UHPLC Fitting L* UHPLC Fitting S Extender Tool for UHPLC Fitting * UHPLC Fitting L is mm longer than UHPLC Fitting S. It is recommended for use when a channel switching valve (FCV-AH/AH/6AH) is installed Sure-Lok PEEK Fittings (Pressure Tolerance: 8-0MPa) The Sure-Lok Fitting is easy to install without spanners. The -piece design can tolerate pressure of 8 MPa, while the -piece fitting, assembled with a ferrule and SUS ring, can tolerate pressure of 0 MPa. Please use the extender tool to tighten the fittings. P/N Description Pack AQ0-8 AQ0-8 AQ0-8 AQ0-8 PEEK Fitting, -, / (8MPa) PEEK Nut, -, / (0MPa) PEEK Ferrule Assembly Aluminum Extender Tool for Sure-Lok Fitting * Handled by Shimadzu GLC Ltd. AQ0-8 AQ0-8 AQ0-8 AQ0-8 Male Nut Fitting Set (Pressure Tolerance: MPa) The male nut fitting set is assembled with two SUS hand-tightened male nuts and two PEEK ferrules with taper-shaped ends. This product can be tightened by hand to connect with columns that tolerate pressure of MPa. P/N Description Pack Male Nut Fitting Set PEEK Ferrule 8
39 Mobile Phase Cleaner for HPLC Ghost Trap DS Ghost Trap DS-HP for UHPLC A new high-pressure model for the elimination of impurities from organic solvents has been added to the Ghost Trap DS* lineup. The Ghost Trap DS was co-developed with Daiichi Sankyo Co., Ltd. It has been designed to effectively adsorb impurities in the mobile phase in order to reduce the time required for method development and impurity analysis. The 0 MPa pressure tolerance of the Ghost Trap DS-HP allows it to be used with UHPLC systems. This permits the effective use of the Ghost Trap DS for method development by UHPLC and subsequent transfer to conventional analysis. * DS: Abbreviation of Daiichi Sankyo (D) and Shimadzu (S) Consistently Traps Impurities, Even in Organic Solvents A major feature of the Ghost Trap DS is the ability to remove impurities, even in organic solvents. When the Ghost Trap DS is installed between the gradient mixer and autosampler in reversed phase gradient analysis, it can trap impurities from the piping and gradient mixer in addition to those from the mobile phase. The example on the right shows that it can effectively trap impurities in mobile phase when the Ghost Trap DS is installed just downstream of the gradient mixer. When the Ghost Trap DS is not used When the Ghost Trap DS is used These gradient ghost peaks are completely removed! : ODS column Mobile Phase : A: mmol/l Phosphate (Potassium) buffer solution ph.0 /Acetonitrile =9/ : B: Water/Acetonitrile = /9 Flow Rate : 0.6 ml/min Oven : ºC Detection : UV nm Example of removing ghost peaks by Ghost Trap DS (The data is provided by Daiichi Sankyo Co., Ltd.) Product Lineup Item P/N Description Dimensions Internal Volume * Pressure Tolerance Ghost Trap DS Cartridge (pcs) Cartridge (pcs) + Holder Cartridge (pcs) Cartridge (pcs) + Holder 0 mml. 7.6 mmi.d..0 mmi.d. mml. Approx. 700 μl Approx. 0 μl MPa Ghost Trap DS-HP Packed type. mmi.d. 0 mml. Approx. 60 μl 0 MPa * Note that a delay volume equivalent to the internal volume of the product occurs if the product is installed downstream of the gradient mixer or the confluence of two pumps. * The product service life differs according to analysis conditions, such as the mobile phase used. * In analysis using an ion-pairing reagent, the ion-pairing reagent may be retained in the product, influencing the retention time and peak shape. * Before connecting the analytical column, be sure to thoroughly clean the flow path with mobile phase (close to the final concentration for gradient analysis). * Note that some impurities may not be removed. * When performing high-pressure analysis exceeding MPa with a UHPLC system, connect the gradient mixer to the Ghost Trap DS-HP with a pipe for UHPLC (e.g ). Shim-pack GIST/GISS/GIS/GWS Series and Normal Phase HPLC 9
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