Experience the Zebron Difference

Transcription

1 Experience the Zebron Difference Premier GC Column Selection Guide

2 Introducing the Zebron Experience Proven Performance Zebron GC columns are engineered by expert Phenomenex GC scientists that created key J&W technologies. Our inventive philosophy and expertise in GC column manufacturing allow us to continuously develop award-winning column chemistries. We provide best-in-class technologies and support to make your analysis easier let the Zebron experience put you first. Our Customers Come First Complimentary Services: Column and accessory selection support Method optimization assistance Thousands of resources and applications online If Zebron columns do not provide you with equivalent or better separations as compared to any other GC column of the same phase and comparable dimensions, send in your comparative data within 45 days and keep the column for FREE!! Phenomenex has always given superb customer support I changed suppliers from Agilent to Phenomenex based on your customer support about 0 years ago and you have not failed me since that point! Marie Coschigano, Genzyme Corporation Continuing Innovation Zebron s track record of innovation has been recognized with 3 R&D 00 Awards. No other GC columns have received this honor. Your input fuels the research and development of GC products designed specifically to overcome your analytical obstacles share with us at Birth of Zebron GC columns 004 Zebron MS phases 005 ZB-WAXplus 00 % water stable 006 ZB-MultiResidue phases For pesticide screening 007 Zebron Inferno Stable up to 430 C 009 ZB-Drug- ZB-Bioethanol 0 Cool-Lock Nut Patented Technology 0 ZB-SemiVolatiles Improved inertness for SVOCs 04 ZB-CLPesticides Versatility for pesticides by GC/ ECD 05 ZB-5MS PLUS The next generation of inertness 06 Phenomenex, Inc. All rights reserved. Phenomenex l WEB:

3 GC Table Accessories of Contents GC Phase Selection Tips... p. 4 GC Column Dimension Selection Tips... p. 5 GC Column - Phase Selection... p. 6 For General Purpose Wide range of general purpose Zebron phases to suit a variety of applications. Every Zebron GC column is individually QC tested for efficiency, bleed, activity, and retention. Polar Semi-Volatiles ZB-ms... p. 7 ZB-5MSPLUS... p. 8 Non-Polar Volatiles ZB p. ZB-WAXPLUS... p. Other Offerings: ZB-, ZB-5, ZB-5MSi, ZB-35, ZB-50, ZB-70, ZB-70P, ZB-WAX, ZB-FFAP, and ZB-XLB For Specialty Applications Premier Zebron phases specially designed and manufactured to deliver the best results for specific application areas Environmental ZB-SemiVolatiles... pp. 3-4 ZB-MultiResidue &... pp. 5-6 Drugs of Abuse ZB-Drug-... p. 7 Simulated Distillation ZB-XT SimDist Metal... p. 8 Other Offerings: ZB-Bioethanol, ZB-CLPesticides- and - For High Temperatures Robust fused silica columns with long lifetimes for high temperature bake outs up to 430 C Featured Zebron Inferno Phases ZB-HT Inferno... p. 9 ZB-5HT Inferno... p. 0 Other Offerings: ZB-35HT Inferno and ZB-XLB-HT Inferno 3

4 Introducing Zebron GC the Columns Zebron Experience GC Phase Selection Tips Selectivity Has the Biggest Impact on Resolution Resolution between two analytes is mainly determined by the selectivity of the stationary phase. By increasing the resolution between two compounds, the total analysis time can often be reduced significantly! The Master Resolution Equation N x α- x R s = 4 α k k+ Efficiency Term Selectivity Term Retention Term Selectivity vs. Polarity Column polarity and selectivity are often confused polarity gives a general guideline for sample capacity and separation, which can affect peak shape and resolution. However, two columns may have similar polarity but show very different separation profiles because of differences in phase chemistry. ZB-35 (Polarity: 8) ZB-70 (Polarity: 9) C N CH 3 CH 3 Si O Si O Si O (CH ) 3 Si O CH 3 CH 3 65 % Dimethylpolysiloxane 35 % Phenyl 86 % Dimethylpolysiloxane The columns have very similar polarity, but the cyanopropyl group makes ZB-70 very different from ZB-35 in terms of selectivity. 4 % Cyanopropylphenyl The 3 Most Prevalent GC Interactions Dispersive Forces (Van der Waals Interactions) Weakest of all intermolecular forces and occurs between non-polar compounds Separation is based on boiling point (classic example hydrocarbon separation in SimDist analysis) Dipole-Dipole Interactions Either permanently present or induced by analyte-stationary phase interactions Higher dipole-dipole interaction can help separate compounds with similar boiling points, but different chemical structures Hydrogen Bonding (Acid-Base Interactions) Can cause poor peak shape or irreversible binding to the inlet liner or to the column itself 4

5 GC Zebron Accessories GC Columns GC Column Dimension Selection Tips A good starting dimension is 30 meter x 0.5 mm x 0.5 µm. From there, you can tweak individual parameters (column length, ID, and film thickness) depending on the needs of your analysis. Shorter (5 m or less) Recommended For: Sample screening or samples with very few analytes LENGTH 30m Longer (60 m or more) Recommended For: Better separation or complex samples with closely eluting peaks Advantages: Faster run times; higher efficiency separations; good for more chemically active samples and high molecular weight compounds Advantages: Higher resolution as length increases; good for low boilers, less active samples, or complex temperature ramps Disadvantages: Resolution decreases as length decreases Disadvantages: Slow run times Narrower (0.0, 0.8, or 0.0 mm) Recommended For: Faster analysis and complex samples INTERNAL DIAMETER 0.5mm Recommended For: Dirty or highly concentrated samples Wider (0.3 or 0.53 mm) Advantages: Faster run times; better resolution Advantages: Increased sample capacity; good for routine on-column injection Disadvantages: Easily overloaded; lower sample capacity Disadvantages: Decreased efficiency; resolution decreases as ID increases Thinner (0.0 or 0.8 µm) Recommended For: Analysis of high boilers, GC-MS applications Advantages: Higher efficiency; lower bleed; faster run times; higher temperature limits FILM THICKNESS 0.5µm Thicker (0.50 µm or more) Recommended For: Analysis of low boilers, gases, solvents, purgeables, and volatile compounds Advantages: Better inertness; higher capacity Disadvantages: Less inert; limited retention Disadvantages: Slower run times; higher bleed; lower temperature limits 5

6 Introducing Zebron GC the Columns Zebron Experience GC Column - Phase Selection Learn even more about Zebron GC column phases and applications at Polarity Phase Composition Temperature Limits (Isothermal/TPGC) MS Certified 5 ZB- 00 % Dimethylpolysiloxane -60 to 360/370 C* 3 5 ZB-ms 00 % Dimethylpolysiloxane -60 to 360/370 C 3 5 ZB-HT Inferno 00 % Dimethylpolysiloxane -60 to 400/430 C** 3 5 ZB-XT SimDist 00 % Dimethylpolysiloxane -60 to 450 C* 3 8 ZB-5 95 % Dimethylpolysiloxane 5 % Phenyl 8 ZB-5ms 95 % Dimethylpolysiloxane 5 % Phenyl-Arylene 8 ZB-5MSi 95 % Dimethylpolysiloxane 5 % Phenyl 5% Pheyl-Arylene 8 ZB-5MSplus 95% Dimethylpolysiloxane 8 ZB-5HT Inferno 95 % Dimethylpolysiloxane 5 % Phenyl 8 ZB-SemiVolatiles 95 % Dimethylpolysiloxane 5 % Phenyl-Arylene -60 to 360/370 C* 3-60 to 35/350 C 3-60 to 360/370 C 3-60 to 35/350 C 3-60 to 400/430 C** 3-60 to 35/350 C 3 9 ZB-XLB Proprietary 30 to 340/360 C* 3 9 ZB-XLB-HT Inferno Proprietary 30 to 400 C 3 ZB-MultiResidue - Proprietary -60 to 30/340 C 3 3 ZB % Dimethylpolysiloxane 6 % Cyanopropylphenyl -0 to 60 C 5 ZB-MultiResidue- Proprietary -60 to 30/340 C 3 8 ZB % Dimethylpolysiloxane 35 % Phenyl 8 ZB-35-HT Inferno 65 % Dimethylpolysiloxane 35 % Phenyl 9 ZB % Dimethylpolysiloxane 4 % Cyanopropylphenyl 9 ZB-70P 86 % Dimethylpolysiloxane 4 % Cyanopropylphenyl 4 ZB % Dimethylpolysiloxane 50 % Phenyl 40 to 340/360 C 3 40 to 400 C 3-0 to 80/300 C* -0 to 80/300 C* 40 to 30/340 C 3 5 ZB-WAXplus 00 % Polyethylene Glycol 0 to 50/60 C* 57 ZB-WAX 00 % Polyethylene Glycol 40 to 50/60 C 3 58 ZB-FFAP Nitroterephthalic Acid Modified Polyethylene Glycol PHENOMENEX ~exclusive~ 40 to 50/60 C ZB-CLPesticides- & Proprietary 40 to 30/340 C ZB-BAC & Proprietary 40 to 30/340 C 3 ZB-Drug- Proprietary -0 to 60/80 C 3 ZB-Bioethanol Proprietary -60 to 340/360 C 3 Need help selecting a column? Ask a GC specialist at Recommended Applications Amines, Drugs, Essential Oils, Ethanol, Gases (Refinery), Hydrocarbons, Mercaptans, MTBE, Natural Gas Odorants, Oxygenates and GROs, PCBs, Pesticides, Semi-volatiles, Simulated Distillation, Solvent Impurities, Sulfur Compounds (Light) Acids, Amines, Diesel Fuel, Drugs, Flavors & Fragrances, PCBs (EPA Method 668), Pesticides Diesel Fuel, High Boiling Petroleum Products, High Molecular Weight Waxes, Long-chained Hydrocarbons, Motor Oils, Polymers/Plastics, Simulated Distillation ASTM Methods (D887, D887X, D370, D635, D769), Crude Oil, Gasoline Fractions, Petroleum Distillates, Petroleum Fractions, Simulated Distillation, Vacuum Distillates Alkaloids, Dioxins, Drugs, Essential Oils/Flavors, FAMEs, Halo-hydrocarbons, PCBs/Aroclors, Pesticides/Herbicides, Phenols, Residual Solvents, Semi-volatiles Acids, Alkaloids, Amines, Dioxins, Drugs, Essential Oils/Flavors, FAMEs, Halo-hydrocarbons, PCBs/Aroclors, Pesticides/Herbicides, Phenols, Residual Solvents, Semi-volatiles, Solvent Impurities Drugs, EPA Methods, FAMEs, Nitrosamines, Pesticides, Phenols Acids, Alkaloids, Amines, Drugs, Essential Oils/ Flavours, Halo-hydrocarbons, Phenols, Residual Solvents, Solvent Impurities, Pesticides/ Herbicides Diesel Fuels, High Boiling Petroleum Products, High Molecular Weight Waxes, Long-chained Hydrocarbons, Motor Oils, Polymers/Plastics, Simulated Distillation, Surfactants, Triglycerides Acids, Alkaloids, Amine, Dioxins, Drugs, EPA Methods (55, 60, 65, 800, 870D), Halogentaed Hydrocarbons, PCB/Aroclors, Pesticides/Herbicides, Phenols, Residual Solvents, SemiVolatiles PCBs, Pesticides/Herbicides EPA Methods, PCBs, Pesticides/Herbicides Aroclors/PCBs, Haloacetic Acids, Herbicides, Insecticides, Multi-Pesticide Screening, Nitrogen Containing Pesticides, Organochlorine Pesticides, Organophosphorous Pesticides EPA Methods (50.3, 50., 503., 54., 60, 60, 64, 800, 805, 800, 80, 840, 860), Pharmaceuticals, Residual Solvents, Volatile Organic Compounds (VOCs) Aroclors/PCBs, Haloacetic Acids, Herbicides, Insecticides, Multi-Pesticide Residue/Screening, Nitrogen Containing Pesticides, Organochlorine Pesticides, Organophosphorous Pesticides Amines, Aroclors, Drugs, EPA Methods (508, 608, 808, 84, 85), Pesticides, Pharmaceuticals, Semi-volatiles, Steroids Amines, Aroclors, Chemicals, Drugs, EPA Methods (508, 608, 808, 84, 85), Pesticides, Pharmaceuticals, Semi-volatiles, Steroids Alcohols, Amines, Aromatic Hydrocarbons, Drugs, Esters, PAHs, PCBs, Pharmaceutical Intermediates, Phenols, Solvents, Steroids, TMS Sugars, Tranquilizers Aroclors, Nitrogen Containing Pesticides, Organochlorine Pesticides, Organophosphorous Pesticides Antidepressants, Aroclors, Cholesterols, Drugs of Abuse, EPA Methods (508, 608, 808, 84, 85), Glycols, Pesticides/Herbicides, Steroids, Triglycerides Alcohols, Aldehydes, Aromatics, Essential Oils, Flavors & Fragrances, Free Fatty Acids, Glycols, OVIs, Pharmaceuticals, Solvents / Residual Solvents, Styrene, Xylene Isomers Alcohols, Aldehydes, Aromatics, Basic Compounds, Essential Oils, Flavors & Fragrances, Glycols, Pharmaceuticals, Solvents, Styrene, Xylene Isomers Acrylates, Alcohols, Aldehydes, Free Fatty Acids, Ketones, Organic Acids, Phenols, Volatile Free Acids Dual-column chlorinated pesticide EPA Methods (808 and 808 extended, 808, 85, 504, 505, 508,55) Abused Inhalant Anesthetics, Blood Alcohol Analysis Drug Screening (6-MAM, Amphetamines, Barbiturates, Benzodiazepines, Opiates, PCP, THC) Alcohol, Bioethanol, Fusel Alcohols 6 * Thicker films (.0 µm df) are rated to lower Isothermal/TPGC limits for this phase. ** 0.53 mm ID dimensions are rated to lower max operational temperature for this phase. For specific temperature limits for your phase, visit or contact your GC Specialist.

7 GC Zebron Accessories GC Columns ZB-ms Very low bleed (MS Certified) phase especially suited to high sensitivity GC/MS Column Profile Low High Extremely inert for active compounds such as drugs, pesticides, or acids and bases Improved signal-to-noise ratio for better sensitivity and mass spectral integrity 5 Polarity Bleed Temperature Limits Identical selectivity to the ZB- Stability Equivalent to USP Phase G Temperature Limits: -60 to 360/370 C (Isothermal/TPGC) Applications Amines Acids Diesel Fuel Polychlorinated Biphenyls (EPA Method 668) Drugs of abuse Flavors & Fragrances Pesticides Lower Overall Column Activity Activity is a key measure of column quality. This is why Zebron ZB-ms columns are aggressively tested to ensure full deactivation. Below is an example of the aggressive QC test mix we use, notice the low tailing for even the most active compounds like -Ethylhexanoic Acid! Alternative to Any MS-Certified 00 % Dimethylpolysiloxane Phase: DB-ms HP-ms AT-ms MDN- Rtx-MS VF-ms SolGel-ms Equity- CP-Sil 5 CB ms Test Conditions for Zebron ZB-ms Column: Zebron ZB-ms Dimensions: 30 meter x 0.5 mm x 0.5 µm Part No.: 7HG-G0- Injection: Split 50 C,.0 µl Carrier Gas: ml/min (constant flow) Oven Program: 40 C (Isothermal) Detector: 35 C Sample:. Decane. -Ethylhexanoic Acid 3. 4-Chlorophenol 4. Naphthalene 5. Tridecane 6. -Undecanol 7. Dicyclohexylamine 8. Pentadecane ZB-ms Test Mix 5 0 min Part No.: AG App ID 5545 Zebron ZB-ms GC Columns ID(mm) df(µm) Temp. Limits C Part No. 0-Meter to 360/370 7CB-G to 360/370 7CD-G0-08 -Meter to 360/370 7DE-G0-4 5-Meter to 360/370 7EG-G to 360/370 7EM-G0-0-Meter to 360/370 7FD-G to 360/370 7FD-G0-5-Meter to 360/370 7GE-G Meter to 360/370 7HG-G to 360/370 7HG-G to 360/370 7HG-G to 360/370 7HG-G to 360/370 7HM-G to 360/370 7HM-G to 360/370 7HK-G0-60-Meter to 360/370 7KG-G to 360/370 7KG-G to 360/370 7KM-G0- Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG- G0--B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages. 7

8 Introducing Zebron GC the Columns Zebron Experience ZB-5MSplus The next generation of inertness for specialty chemical, forensic, toxicology, and food testing applications Specialized deactivation for versatile 5% Phenyl-Arylene selectivity with improved sensitivity Low bleed (MS Certified) and well-suited to high sensitivity GC/MS and GC/MS/MS work Equivalent to USP Phase G7 Temperature Limits: -60 to 35/350 C Column Profile Low Polarity Bleed Temperature Limits Stability 8 High Zebron ZB-5MS PLUS Better peak shapes with no retention time shifts! Applications Acids Alkaloids Amines Drugs Essential Oils/Flavors Halo-hydrocarbons Phenols Residual Solvents Solvent Impurities Pesticides/Herbicides Upgrade from Any 5 % Phenyl or 5 % Phenyl -Arylene 95 % Dimethylpolysiloxane Phase: DB -5ms HP -5ms Rxi -5Sil MS DB-5ms Ultra Inert HP-5ms Ultra Inert SLB -5ms DB-5.65 VF-5ms Traditional 5ms Phase App ID 348 min App ID 349 min Zebron ZB-5MSPLUS GC Columns ID(mm) df(µm) Temp. Limits C Part No. 5-Meter to 35/350 7EG-G030-0-Meter to 35/350 7FD-G to 35/350 7FD-G Meter to 35/350 7HG-G to 35/350 7HG-G to 35/350 7HG-G to 35/350 7HM-G to 35/350 7HM-G Meter with 5-Meter Guardian Integrated Guard to 35/350 7HG-G030--GGA 30-Meter with 0-Meter Guardian Integrated Guard to 35/350 7HG-G030--GGC to 35/350 7HG-G030-7-GGC 60-Meter to 35/350 7KG-G030- Conditions for both columns: Column: As listed Dimensions: 30 meter x 0.5 mm x 0.5 µm Injection: Split 50 C, µl Carrier Gas: ml/min (constant flow) Oven Program: 40 C (Isothermal) Detector: 35 C Sample:. m-xylene. o-xylene 3. Decane 4. -Ethylhexanoic Acid 5.,6-Hexanediol 6. 4-Chlorophenol 7. Tridecane 8. -Methylnaphthalene 9. -Undecanol 0. Tetradecane. Dicyclohexylamine. Pentadecane ZB-5MSPLUS Test Mix Part No.: AG0-90 Want better peak shape for acidic/basic compounds, drugs of abuse, and pesticides? Try the highly inert and low bleed ZB-5MSi 8

9 GC Zebron Accessories GC Columns ZB-HT Inferno 007 R&D 00 Award Recipient First non-metal columns stable to 430 C Provides true boiling point separation for hydrocarbon distillation methods Longer lifetime with rugged high temperature, polyimide coated, fused silica tubing Low activity, provides good peak shape for acidic and basic samples Provides robust column performance for high temperature bake outs Individually tested for low bleed, MS certified Temperature Limits: -60 to 400/430 C (Isothermal/TPGC)* Column Profile Low Polarity Bleed Temperature Limits Stability 5 Applications High Boiling Petroleum Products Simulated Distillation Methods Long-chained Hydrocarbons High Molecular Weight Waxes Polymers/Plastics Motor Oils Diesel Fuel High Hydrocarbons from Water: DIN EN ISO (DEV H53) Alternative to Any 00 % Dimethylpolysiloxane High Temperature Phase: DB-ht Petrocol 887 CP-SimDist Rxi -HT min Column: Zebron ZB-HT Inferno Dimensions: 5 meter x 0.3 mm x 0.5 μm Part No.: 7EM-G04- Injection: 300 C, 0 μl Carrier Gas: ml/min (constant flow) Oven Program: 50 C for min to C/min for 5 min Detector: 330 C Sample:. Decane. Tetracontane App ID 853 Zebron ZB-HT Inferno GC Columns ID(mm) df(µm) Temp. Limits C Part No. 5-Meter to 400/430 7AK-G Meter to 400/430 7CM-G04-5-Meter to 400/430 7EG-G to 400/430 7EG-G to 400/430 7EM-G to 400/430 7EM-G to 400 7EK-G Meter to 400/430 7FD-G Meter to 400/430 7HG-G to 400/430 7HG-G to 400/430 7HM-G to 400/430 7HM-G to 400 7HK-G04-05 Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG- G04--B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages. ZB-HT Test Mix Part No.: AG0-555 Courtesy of Dr. Alexander Ruderisch, Agrolab Labor GmbH, Bruckberg *0.53 mm ID columns are rated to 400 C max operational temperature. 9

10 Introducing Zebron GC the Columns Zebron Experience ZB-5HT Inferno 007 R&D 00 Award Recipient 0 First non-metal columns stable to 430 C Robust column for high temperature bake outs analysis, such as biodiesel, long-chain hydrocarbons, polymers, and high molecular weight compounds Provides true boiling point separation for hydrocarbon distillation methods Longer lifetime with rugged high temperature, polymide coated, fused silica tubing Low activity, provides good peak shape for acidic and basic samples Individually tested for low bleed, MS certified Temperature Limits: -60 to 400/430 C (Isothermal/TPGC)* Lower Bleed!* pa Zebron ZB-5HT Inferno Agilent J&W DB-5ht min Conditions for all columns**: Dimensions: 30 meter x 0.5 mm x 0.0 μm Injection: Null 50 C Carrier Gas: psi (Constant Pressure) Oven Program: 0 C for 3 min to C/min (hold 5 min) to C/min (hold 5 min) to C/min (hold 5 min) to C/min (hold 5 min) to C/min (hold 5 min) Detector: 405 C How does the lifetime test work? Hydrocarbons are a good way to measure the stability and lifetime of a non-polar column. Because its interaction with the phase is mostly based on London Dispersion forces, any change in retention time is correlated with phase loss. This will result in increased bleed and poor reproducibility. In the lifetime study above, the Zebron ZB-5HT Inferno column has twice the lifetime as the two comparative columns. For the test, all columns were held at 400 C for hours and then the oven was lowered to 0 C for Pentadecane analysis. The Varian HT column broke just after 40 hours at 400 C. The Agilent J&W DB- 5ht column had the same retention for Pentadecane at 40 hours as the ZB-5HT at 00 hours. *The shaded area depicts the bleed criteria for MS certified columns on a MS detector. MS bleed certification values are typically read at 30 ºC. This demonstrates the low bleed capabilities of the ZB-5HT. It meets MS certification limits even at 360 ºC! **All columns used for above tests were new/never used, prior to this testing and purchased either directly from the original manufacturer or through an authorized distributor. All testing was carefully controlled to ensure conditions were similar for all columns involved. The comparative data may not be representative of every application. Column Profile Low Polarity Bleed Temperature Limits Stability Zebron Inferno Columns Win In The Lifetime Test Retention of Pentadecane at 0 C (min) Comparison of retention of Pentadecane between the Zebron ZB-5HT Inferno column versus a traditional 5 %-Phenyl - 95 %-dimethylpolysiloxane column. Note that the 5 %-Phenyl column died around 40 hours at 400 C whereas the Zebron ZB-5HT Inferno column maintained great retention of Pentadecane over 00 hours Hours at 400 C Conditions for all columns**: Dimensions: 30 meter x 0.5 mm x 0.0 μm Injection:.0 μl of test mix AG Carrier Gas: ml/min (constant flow) Oven Program: 0 C Isothermal Detector: Flame Ionization Detector 400 C Sample: Pentadecane Zebron ZB-5HT Inferno Agilent J&W DB-5ht Varian VF-5ht Zebron ZB-5HT Inferno GC Columns ID(mm) df(µm) Temp. Limits C Part No. 0-Meter with -Meter Spliced Guard (0.53 mm ID) to 400/430 7CM-G05-0-GST 5-Meter to 400/430 7EG-G to 400/430 7EG-G to 400/430 7EM-G to 400/430 7EM-G to 400 7EK-G Meter with -Meter Spliced Guard (0.53 mm ID) to 400/430 7EM-G05-0-GST 0-Meter to 400/430 7FD-G Meter to 400/430 7HG-G to 400/430 7HG-G to 400/430 7HM-G to 400/430 7HM-G to 400 7HK-G Meter to 400/430 7KG-G05- Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G05- -B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages. ZB-5HT Test Mix Part No.: AG0-555 *0.53 mm ID columns are rated to 400 C max operational temperature. Agilent and J&W are registered trademarks of Agilent Technologies, Inc. Varian is a registered trademark under license to Agilent Technologies, Inc. High

11 GC Zebron Accessories GC Columns ZB-64 Widely used phase to separate volatile organic flavor and fragrance additives and residual solvents in industrial or pharmaceutical products (OVIs) Popular choice for residual solvent testing Excellent for US EPA Methods 50.3, 50., 503., 54., 60, 60, 64, 800, 805, 800, 80, 840, 860 Specifically designed for the separation of volatile organic compounds (VOCs) Increased temperature limit speeds run times and re-equilibration Equivalent to USP Phase G43 Temperature Limits: -0 to 60 C Column Profile Low Polarity Bleed Temperature Limits Stability 3 Applications Volatile Organic Compounds (VOCs) Residual Solvents EPA Methods 50.3, 50., 503., 54., 60, 60, 64, 800, 805, 800, 80, 840, 860 Pharmaceuticals High USP <467>: Residual Solvents Procedure A - Class 4 Alternative to Any 6 %-Cyanopropylphenyl- 94 %-Dimethylpolysiloxane Phase: DB-64 AT-64 DB-VRX Rtx-30 CP-30 Rtx-64 BP HP-VOC DB-30 SPB-30 CP-Select 64 CB SPB-64 Rtx-VMS min Column: Zebron ZB-64 Dimensions: 30 meter x 0.3 mm x.80 µm Part No.: 7HM-G005-3 Injection: Split 40 C, ml Carrier Gas: 35 cm/sec (constant flow) Oven Program: 40 C for 0 min to 40 0 C/min for 0 min Detector: 50 C Sample:.,-Dichloroethene.,,-Trichloroethane 3. Carbon tetrachloride 4. Benzene 5.,-Dichloroethane 3 5 App ID 6348 Zebron ZB-64 GC Columns ID(mm) df(µm) Temp. Limits C Part No. 0-Meter to 60 7FD-G Meter to 60 7HG-G to 60 7HM-G to 60 7HK-G Meter to 60 7KG-G to 60 7KM-G to 60 7KK-G Meter to 60 7LK-G Meter to 60 7NK-G Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HM- G005-3-B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages. ZB-64 Test Mix Part No.: AG0-559

12 Introducing Zebron GC the Columns Zebron Experience ZB-WAXplus 00 % aqueous stable, excellent for aqueous samples Extremely inert for acidic compounds Enhanced selectivity for low boiling solvents High retention of alcohols and chlorinated solvents Increased efficiency at 0 C Bonded and solvent rinsible Equivalent to USP Phase G6 Temperature Limits: 0 to 50/60 C (Isothermal/TPGC)* Enhanced Aqueous Stability for Polar Compounds Wax columns provide optimal selectivity for many aqueous soluble compounds such as those found in alcoholic beverages or glycol samples. Historically, Polyethylene Glycol (PEG) phases have been unstable with aqueous samples resulting in poor reproducibility and decreased lifetime. The ZB-WAXplus bonding procedure results in exceptional stability to repeated injections of aqueous matrices. Water Reproducibility of ZB-WAXPLUS pa Column: Zebron ZB-WAXplus Dimensions: 30 meter x 0.5 mm x 0.5 µm Part No.: 7HG-G03- Injection: Split 40 C, 0. µl Carrier Gas: ml/min (constant flow) Oven Program: 35 C for 5 min to 85 0 C/min to 00 5 C/min for min Detector: 00 C Sample:. Acetaldehyde. Ethyl Acetate 3. Methanol 4. Ethanol 5. Propanol 6. Isobutanol 7. -Methylbutanol 8. 3-Methylbutanol 6 7,8 Scottish Single Malt Whiskey 60% Aqueous! min App ID 587 Column Profile Low Polarity Bleed Temperature Limits Stability Applications Alcoholic Beverages Alcohols OVIs Aldehydes Aromatics Alternative to Any Polyethylene Glycol Phase: DB-WAX PEG 0M HP-0M Stabilwax Acids (free) Essential oils Flavors / Fragrances Styrene Glycols SUPELCOWAX-0 AT-WAX CP-WAX 5 CB AT-AquaWax 5 Pharmaceuticals Solvents Xylene Isomers High Carbowax 0M Permabond CW 0M CAM BP0 Zebron ZB-WAXPLUS GC Columns ID(mm) df(µm) Temp. Limits C Part No. 0-Meter to 50/60 7CB-G Meter to 50/60 7EG-G to 30/40 7EK-G03-0-Meter to 50/60 7FD-G Meter to 50/60 7HG-G to 50/60 7HG-G to 50/60 7HM-G to 50/60 7HM-G to 30/40 7HM-G to 30/40 7HK-G03-60-Meter to 50/60 7KG-G to 50/60 7KG-G to 50/60 7KG-G to 50/60 7KM-G to 50/60 7KM-G to 30/40 7KK-G03- Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG- G03--B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages. ZB-WAXplus Test Mix Part No.: AG *Thicker films (.0 µm df) are rated to 30/40 C (Isothermal/TPGC).

13 GC Zebron Accessories GC Columns ZB-SemiVolatiles Specifically designed to overcome obstacles for sensitive semi-volatile methods Column Profile Low High Enviro-Inert Technology provides a rugged 5 %-Phenyl- Arylene phase improve inertness without compromising selectivity Polarity Bleed 8 Rugged QC test includes EPA 870 tuning standard to ensure column is ready to pass suitability requirements Temperature Limits Stability Low bleed (MS Certified) especially suited to high sensitivity work using GC/MS Applications Our column of choice for EPA Methods 55, 60, 65, 800, and 870 Temperature Limits: -60 to 35/350 C Acids Alkaloids Amine Dioxins Drugs EPA Methods (55, 60, 65, 800, 870D) Halogentaed Hydrocarbons PCB/Aroclors Pesticides/Herbicides Phenols Residual Solvents SemiVolatiles Upgrade from Any 5%-Phenyl or 5%-Phenyl -Arylene-95%- Dimethylpolysiloxane Phase: DB-5ms HP-5ms Rxi-5ms DB-5ms Ultra Inert HP-5ms Ultra Inert Rxi-5Sil MS DB-5.65 VF-5ms CP-Sil 8 CB MS You Spoke, You Tested, You Approved Your input fueled the research and development of Zebron ZB-SemiVolatiles the column specifically designed to overcome your EPA Method 870D obstacles. Several environmental labs have verified real-world performance on ZB-SemiVolatiles: We QC Test for the SVOC Compounds You Analyze We take the guesswork out of meeting method requirements by aggressively testing ZB-SemiVolatiles with two different test mixes. We incorporated troublesome analytes from your samples (Pyridine) and compounds in the 870D tuning standard (DDT, Pentachlorophenol, and Benzidine) into our QC test, so you can be sure your column is ready to meet suitability requirements for the method. TestAmerica Laboratories, Inc. Buffalo Phoenix Environmental Laboratories, Inc. Other labs like yours! Real Customer Results For EPA Method 870D on ZB-SemiVolatiles Pyridine (PYR) Very active amine exposes any basic activity EPA 870D Requirement Our Requirement Peak Response: Not Specified 0.6* Pentachlorophenol (PCP) PCP peaks disappear and exhibit tailing on columns with acid activity EPA 870D Requirement Our Requirement Peak Skew:.0.0 Peak Response: Not Specified 0.3 Benzidine Active amine that tails when basic activity is present EPA 870D Requirement Our Requirement Peak Skew:.0.0 Zebron ZB-SemiVolatiles is a very stable and durable semivolatile column. This has reduced TestAmerica s downtime and increased our productivity, enabling us to better serve our clients needs. DDT Breaks down in an active system to DDE & DDD EPA 870D Requirement Our Requirement Breakdown: < 0 % < 0 % *Requirement of 0.5 for 60 m x 0.5 mm x 0.5 µm and 0 m Guardian dimensions The opinions stated herein are solely those of the speaker and not necessarily those of any company or organization. 3

14 Introducing Zebron GC the Columns Zebron Experience min min ZB-SemiVolatiles (cont d) Great Resolution of Key Critical Pairs and Improved Peak Shapes Enviro-Inert technology allows Zebron ZB-SemiVolatiles to provide improved productivity with shorter run times for EPA 870D, while maintaining resolution of key critical pairs. Full Separation Good Peak Shape min min min EPA Method 870D: Semi-volatile Organic Compounds min.5 min min..3 N-Nitrosodimethylamine and Pyridine,4-Dinitrophenol App ID 058 Valley Height = 9.9 % 870D Requires 50 % BOX THAT IS " TALL BY.6" WIDE (USED AS TEMPLATE) Valley Height = 0.9 % 870D Requires 50 % min min min min min.7.9 min min Benzo[b]fluoranthene and Benzo[k]fluoranthene Indeno[,,3-cd]pyrene and Dibenz[a,h]anthracene, both share mass 76 BOX THAT IS " TALL BY.6" WIDE (USED AS TEMPLATE) min min min Real Customer Sample for EPA Method 870D Zebron ZB-SemiVolatiles GC Columns ID (mm) df (µm) Temperature Limits ( C) Part No. 5-Meter to 35/350 7EG-G to 35/350 7EG-G Meter to 35/350 7FD-G to 35/350 7FD-G Meter to 35/350 7HG-G to 35/350 7HG-G Meter with 5-Meter Guardian Integrated Guard to 35/350 7HG-G07--GGA to 35/350 7HG-G07-7-GGA 30-Meter with 0-Meter Guardian Integrated Guard to 35/350 7HG-G07--GGC to 35/350 7HG-G07-7-GGC 60-Meter to 35/350 7KG-G07- BOX THAT IS " TALL BY.6" WIDE (USED AS TEMPLATE) The opinions stated herein are solely those of the speaker and not necessarily those of any company or organization. 4

15 GC Zebron Accessories GC Columns ZB-MultiResidue - and - exclusive Proprietary phase specially designed for the separation of all types of pesticides, herbicides, and insecticides Column Profile Low High Provides baseline resolution and confirmation of all 0 chlorinated pesticides regulated under EPA Method 808 in 0 min Polarity MR- Polarity MR- 5 Low activity, decreased breakdown of sensitive pesticides such as DDT Bleed Temperature Limits Provides robust column performance for high temperature bake outs MS Certified phases provide low bleed performance for pesticide confirmation by MS Temperature Limits: -60 to 30/340 C (Isothermal/TPGC) Stability Applications Organochlorine Pesticides Insecticides Organophosphorous Pesticides Aroclors/PCBs Nitrogen Containing Pesticides Haloacetic Acids Herbicides Multi-pesticide Residue Methods Similar* to: Rtx-CLPesticides Rtx-CLPesticides Stx-CLPesticides Stx-CLPesticides *not exact equivalent, selectivity might be different EPA Methods 508 and 808A A , , , 0 9, , Baseline Resolution of EPA Method 808 MR App ID 680 B , 9,0 MR App ID min min Column: A) Zebron MultiResidue- B) Zebron MultiResidue- Dimensions: A) 30 meter x 0.3 mm x 0.5 µm B) 30 meter x 0.3 mm x 0.50 µm Part No.: A) 7HM-G07- B) 7HM-G06-7 Injection: 3 C, µl Carrier Gas: ml/min (constant flow) Oven Program: 0 C for 0.5 min to 0 30 C/min to C/min for min Detector: 340 C Sample: All analytes are 50 ppb in isooctane. DBCP. Hexachlorocyclopentadiene 3. Etridiazole 4. Chloroneb 5. Propachlor 6. Diallate 7. a-bhc 8. Hexachlorobenzene 9. g-bhc 0. b-bhc. Dichlone. Alachlor 3. d-bhc 4. Chlorothalonil 5. Heptachlor 6. DCPA (Dacthal) 7. Aldrin 8. Heptachlor epoxide 9. Captan 0. trans-chlordane (gamma). cis-chlordane (alpha). trans-nonachlor 3. Endosulfan I 4. DDE 5. Dieldrin 6. Chloropropylate 7. Chlorobenzilate 8. Endrin 9. Nitrofen 30. DDD 3. Endosulfan II 3. Kepone 33. Trithion 34. Endrin aldehyde 35. DDT 36. Endosulfan sulfate 37. Captafol 38. Methoxychlor 39. Endrin ketone 40. Permethrin 4. Permethrin isomer Well-suited for Dual Column Analysis Injector 5 m Z-Guard Zebron MR- Zebron MR- Y-Splitter ECD Detector ECD Detector 5

16 Introducing Zebron GC the Columns Zebron Experience ZB-MultiResidue - and - (cont d) Zebron ZB-MultiResidue GC Columns (MR)- ID(mm) df(µm) Temp. Limits C Part No. 0-Meter to 30/340 7FD-G Meter to 30/340 7HG-G to 30/340 7HM-G to 30/340 7HM-G to 30/340 7HK-G06-7 Zebron ZB-MultiResidue GC Columns (MR)- ID(mm) df(µm) Temp. Limits C Part No. 30-Meter to 30/340 7HG-G to 30/340 7HM-G to 30/340 7HK-G07-7 Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G06- -B or 7HG-G07-0-B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages. ZB-MultiResidue Column Kits 0.5 mm ID (kit consists of products below) Part No.: KG0-837 Description Dimension Part No. MultiResidue MR- Column 30 meter x 0.5 mm x 0.5 µm df 7HG-G06- MultiResidue MR- Column 30 meter x 0.5 mm x 0.0 µm df 7HG-G07-0 Z-Guard 5 meter x 0.5 mm 7AG-G GZ0 Universal Capillary Column Y-connector, Fused Quartz AG mm ID (kit consists of products below) Part No.: KG0-838 Description Dimension Part No. MultiResidue MR- Column 30 meter x 0.3 mm x 0.50 µm df 7HM-G06-7 MultiResidue MR- Column 30 meter x 0.3 mm x 0.5 µm df 7HM-G07- Z-Guard 5 meter x 0.3 mm 7AM-G GZ0 Universal Capillary Column Y-connector, Fused Quartz AG mm ID (kit consists of products below) Part No.: KG0-839 Description Dimension Part No. MultiResidue MR- Column 30 meter x 0.53 mm x 0.50 µm df 7HK-G06-7 MultiResidue MR- Column 30 meter x 0.53 mm x 0.50 µm df 7HK-G07-7 Z-Guard 5 meter x 0.53 mm 7AK-G GZ0 Universal Capillary Column Y-connector, Fused Quartz AG

17 GC Zebron Accessories GC Columns Zebron ZB-Drug- exclusive 009 R&D 00 Award Recipient Optimized phase for the separation of drugs of abuse Provides fast analysis with great peak shape Improves resolution of target analytes from matrix interferences Specially deactivated to improve quantitation for drug compounds Temperature Limits: 40 to 30/340 C Applications Drugs of Abuse Opiates on a ZB-5ms 3,4 Opiates on a ZB-Drug-, Unique Selectivity for Drugs 3,4 App ID 6733 App ID min Column: Zebron ZB-5ms Dimensions: 0 meter x 0.8 mm x 0.8 µm Part No.: 7CD-G00-08 Injection: Split 40 C, µl Carrier Gas: ml/min (constant flow) Oven Program: 40 C to 30 0 C for min Detector: 30 C Sample: 0 pppm BSTFA/Pyridine. Codeine-d6. Codeine 3. Morphine-d3 4. Morphine min Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part No.: 7CD-G03-08 Injection: Split 80 C, µl Carrier Gas: 55 cm/sec (constant flow) Oven Program: 80 C to C/min Detector: 30 C Sample: 0 pppm BSTFA/Pyridine. Codeine-d6. Codeine 3. Morphine-d3 4. Morphine Amphetamines App ID 7809 Zebron ZB-Drug- GC Columns ID(mm) df(µm) Temp. Limits C Part No. 0-Meter to 30/340 7CD-G Meter to 30/340 7EG-G03-30-Meter to 30/340 7HG-G03- Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG- G03--B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages min Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part No.: 7CD-G03-08 Injection: Split 80 C, 0. µl Carrier Gas: 55 cm/sec (constant flow) Oven Program: 70 C to 30 0 C/min to ºC/min Detector: 30 C Sample: Analytes are 40 ppm derivatized with HFBA. Amphetamine-d 6. MDA. Amphetamine 7. MDMA-d5 3. Methamphetamine-d4 8. MDMA 4. Methamphetamine 9. MDEA-d5 5. MDA-d5 0. MDEA ZB-Drug- Test Mix Part No.: AG

18 Introducing Zebron GC the Columns Zebron Experience ZB-XT SimDist Metal New Glass Infusion technology provides higher efficiency and greater column-to-column reproducibility Guaranteed high quality - every column is individually tested Up to 70% more efficiency than other columns Improved resolution of C50/C5 Increased accuracy for high temperature simulated distillation analysis Temperature Limits: -60 to 450 C (Isothermal/TPGC)* Hydrocarbons C7 C00+: ASTM Method D769 Column Profile Low Polarity Bleed Temperature Limits Stability 5 Applications Crude Oil Vacuum Distillates Petroleum Distillates Petroleum Fractions Gasoline Fractions Simulated Distillations ASTM Methods D887, D887X, D370, D635, D769 High C7 C8 C9 High Efficiency! Resolution: 4.8 Alternative to: Varian CP-SimDist UltiMetal Restek MXT -HT SimDist Agilent J&W DB-HT Sim Dis C70 C80 C90 C00 App ID 964 C60 C44 C5 C min Column: Zebron ZB-XT SimDist Dimensions: 5 meter x 0.53 mm x 0.5 µm Part No.: 7AK-G06-05 Injection: 33 C, µl Carrier Gas: 7 ml/min (constant flow) Oven Program: 30 C to C/min for 0 min Detector: 450 C Sample: C7 to C44 hydrocarbons and POLYWAX 655 Note: Sample in CS. Chromatogram is baseline subtracted. Zebron ZB-XT SimDist Metal ID(mm) df(µm) Temp. Limits C Part No. 5-Meter to 450 7AK-G to 450 7AK-G Meter to 450 7CK-G to 450 7CK-G to 400 7CK-G Meter to 450 7EK-G06- If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7CK-G06-05-B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages. ZB-XT SimDist Test Mix Part No.: AG *Thicker film (.65 µm df) is rated to 400 C (Isothermal/TPGC). 8

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