Quick and Simple Cleanup for. Medicinal Chemistry. It s BIG.

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1 Quick and Simple Cleanup for Medicinal It s IG.

2 Guaranteed Cleanup of Post Synthesis Reactions Strata -XL Giga s are designed to make Medicinal easy! With Strata Giga tubes I ve been able to purify a compound containing a quaternary amine successfully. I would recommend Strata for sure! - Maxim Maheux, TransITech Quick and simple cleanup procedures s available for a wide range of sample sizes; 1 ml up to 150 ml Pre-packed tubes eliminate the excess maintenance involved with cleaning and handling glass packed flash columns Large particle sizes for fast flow Robust polymeric material for ph and chemical resistance, including DMS If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE Phenomenex, Inc. ll rights reserved.

3 Customize Your Cleanup in 2 Easy Steps step 1: Select a Choose from 5 different sorbent chemistries. Cleanup Strategy Desalt neutral compounds Cleanup basic compounds Cleanup strongly basic compounds Cleanup acidic compounds Cleanup strongly acidic compounds Recommendation Strata -XL Strata-XL-C Strata-XL-CW Strata-XL- Strata-XL-W step 2: Choose a Use the chart below to determine proper sorbent mass based on your sample size. Strata-XL Neutral Compound Loading Capacity Recommended 185 mg 460 mg 935 mg 1.85 g 4.65 g 2 g 5 g 10 g 20 g 50 g Strata-XL-C, Strata-XL-CW, Strata-XL- and Strata-XL-W Ionic Compound Loading Capacity Recommended 200 mg 500 mg 1 g 2 g 5 g 10 g 3

4 ) Cleanup asic Compounds chieve high recoveries of basic compounds with Strata -XL-C and Strata -XL-CW. Purify 1, 2, and 3 mines with Strata-XL-C )n S - H + 1 Load reaction mixture Sulfonic cid ligand offers selectivity for the retention of weaker bases Strata-XL-C for weakly basic compounds Target nalytes asic products with a pk a < 10.5 (1, 2, and 3 amines) -C enzenesulfonic acid conjugated to a large particle polymer which has been optimized for gravity flow ases Particle Size 100 μm N cids Neutrals ph Stability 1-14 Ionic Capacity 1 meq/g 2 3 Wash away acids and neutrals Elute purified basic compounds N N 4

5 ) Cleanup asic Compounds Load reaction mixture 1 Purify 1, 2, 3, and 4 mines with Strata -XL-CW )n H Carboxylic cid ligand offers selectivity for the retention of strong bases Strata-XL-CW for strongly basic compounds ases -CW Target nalytes asic products with a pk a > 8 (1, 2, 3, and 4 amines) Carboxylic acid group conjugated to a large particle polymer which has been optimized for gravity flow N cids Neutrals Particle Size 100 μm ph Stability 1-14 Ionic Capacity 0.76 meq/g 2 3 Wash away acids and neutrals Elute purified basic compounds N N 5

6 Cleanup Neutral Compounds Ion-Exchange Method Quickly and easily remove ionic species from Neutral compounds by performing an ion-exchange cleanup. Remove asic ND cidic Compounds with Strata -W 1 Load reaction mixture Si S H Si NH 2 Strong cation - exchange and weak anion - exchange groups allow neutral compounds to flow through the sorbent while ionic compounds are trapped in the sorbent Strata W for neutral compounds Target nalytes Ionically charged compounds, neutral compounds flow through sorbent N ases cids Neutrals Particle Size Sulfonic cid and mino groups for retention of basic and acidic compounds, respectively 55 μm ph Stability N N N 2 Elute purified neutral compounds N N N N 6

7 ) Cleanup Neutral Compounds Reversed Phase/Desalting Method Load reaction mixture 1 Desalt Neutral Compounds with Strata -XL )n Ṅ. Modified pyrrolidone ligand for retention of neutral compounds Strata-XL for neutral compounds Target nalytes Neutral, hydrophobic compounds N ases cids Neutrals N-vinylpyrrolidone conjugated to a large particle polymer which has been optimized for gravity flow Particle Size 100 μm 2 3 ph Stability 1-14 Wash away acids and bases Elute purified neutral compounds N N N N 7

8 ) ) Cleanup cidic Compounds chieve superior cleanup and high recoveries of acidic compounds with Strata -XL- and Strata -XL-W. Purify Weakly cidic Compounds with Strata -XL- 1 Load reaction mixture Purify Strongly cidic Compounds with Strata -XL-W n ) CH 3 CI - N + )n NH NH 2 CH 3 Di-methylbutyl quaternary amine ligand offers selectivity for the retention of weak acids Strata-XL- for weakly acidic compounds Target nalytes cidic compounds with a pk a >3 (carboxylic acids) Di-methylbutyl quaternary amine ligand conjugated to a large particle polymer which has been optimized for gravity flow - Di-amino ligand offers selectivity for the retention of strong acids Strata-XL-W for strongly acidic compounds Target nalytes cidic compounds with a pk a <5 (carboxylic and sulfonic acids) Di-amino ligand conjugated to a large particle polymer which has been optimized for gravity flow Particle Size 100 μm Particle Size 100 μm ph Stability 1-14 Ionic Capacity 0.30 meq/g 2 3 ph Stability 1-14 Ionic Capacity 0.60 meq/g Wash away bases and neutrals Elute purified acidic compounds N N ases cids N Neutrals 8

9 Traditional Cleanup Solutions Silica-based sorbents have been used to fractionate and cleanup synthetic organic compounds for many years. Strata silica-based products are made from the highest quality silica and each batch is quality tested for purity, recovery, and flow to ensure that your results are reproducible. Compound to be Fractionated Recommendation Reversed Phase Solutions Very hydrophobic compounds Hydrophobic compounds Hydrophobic, aromatic compounds Strata C8 Strata C18-E Strata Phenyl Normal Phase Solutions Polar compounds including those that contain a hydroxyl group Polar compounds including those that contain an amino group Strata NH 2 Strata Silica dditional Cleanup Solutions Removal of aqueous residues Strata Sodium Sulfate Strata W, Strata C8, Strata C18-E, Strata Phenyl, Strata NH 2, and Strata Silica Maximum Loading Capacity of Neutral Compounds 100 mg 250 mg 500 mg 1 g 3.5 g 2 g 5 g 10 g 20 g 70 g 9

10 rdering Information Cleanup Solutions Ion-Exchange - Strata -XL-C 2 g 12 ml 8-S044-KDG 20/box 2 g 20 ml 8-S044-KEG 20/box 5 g 20 ml 8-S044-LEG 20/box 5 g 60 ml 8-S044-LFF 16/box 10 g 60 ml 8-S044-MFF 16/box Ion-Exchange - Strata-XL-W 2 g 12 ml 8-S051-KDG 20/box 2 g 20 ml 8-S051-KEG 20/box 5 g 20 ml 8-S051-LEG 20/box 5 g 60 ml 8-S051-LFF 16/box 10 g 60 ml 8-S051-MFF 16/box Ion-Exchange - Strata-XL-CW 2 g 12 ml 8-S052-KDG 20/box 2 g 20 ml 8-S052-KEG 20/box 5 g 20 ml 8-S052-LEG 20/box 5 g 60 ml 8-S052-LFF 16/box 10 g 60 ml 8-S052-MFF 16/box Reversed Phase - Strata-XL 2 g 12 ml 8-S043-KDG 20/box 2 g 20 ml 8-S043-KEG 20/box 5 g 20 ml 8-S043-LEG 20/box 5 g 60 ml 8-S043-LFF 16/box 10 g 60 ml 8-S043-MFF 16/box 20 g 60 ml 8-S043-VFF 16/box 50 g 150 ml 8-S043-YSN 8/box Ion-Exchange - Strata-XL- 2 g 12 ml vailable in ugust 20/box * 2 g 20 ml vailable in ugust 20/box * 5 g 20 ml vailable in ugust 20/box * 5 g 60 ml vailable in ugust 16/box * 10 g 60 ml vailable in ugust 16/box * *Contact Phenomenex for more information. 10

11 rdering Information Traditional Cleanup Solutions Mixed Mode - Strata W 2 g 12 ml 8-S030-KDG 20/box 5 g 20 ml 8-S030-LEG 20/box 10 g 60 ml 8-S030-MFF 16/box 20 g 60 ml 8-S030-VFF 16/box 70 g 150 ml 8-S030-ZSN 8/box Reversed Phase - Strata C8 2 g 12 ml 8-S005-KDG 20/box 5 g 20 ml 8-S005-LEG 20/box 10 g 60 ml 8-S005-MFF 16/box Reversed Phase - Strata Phenyl 2 g 12 ml 8-S006-KDG 20/box Normal Phase - Strata NH mg 12 ml 8-S009-HDG 20/box 2 g 12 ml 8-S009-KDG 20/box 5 g 20 ml 8-S009-LEG 20/box 10 g 60 ml 8-S009-MFF 16/box 20 g 60 ml 8-S009-VFF 16/box 70 g 150 ml 8-S009-ZSN 8/box Reversed Phase - Strata C18-E 500 mg 12 ml 8-S001-HDG 20/box 1 g 20 ml 8-S001-JEG 20/box 2 g 12 ml 8-S001-KDG 20/box 5 g 20 ml 8-S001-LEG 20/box 10 g 60 ml 8-S001-MFF 16/box 20 g 60 ml 8-S001-VFF 16/box 20 g 150 ml 8-S001-VSN 8/box 50 g 150 ml 8-S001-YSN 8/box 70 g 150 ml 8-S001-ZSN 8/box If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE. Normal Phase - Strata Silica 500 mg 12 ml 8-S012-HDG 20/box 1 g 12 ml 8-S012-JDG 20/box 2 g 12 ml 8-S012-KDG 20/box 5 g 20 ml 8-S012-LEG 20/box 10 g 60 ml 8-S012-MFF 16/box 20 g 60 ml 8-S012-VFF 16/box 50 g 150 ml 8-S012-YSN 8/box 70 g 150 ml 8-S012-ZSN 8/box dditional Cleanup - Strata Sodium Sulfate 1 g 6 ml 8-S124-JCH 30/box 1 g 20 ml 8-S124-JEG 20/box 5 g 20 ml 8-S124-LEG 20/box Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at Trademarks Strata is a registered trademark of Phenomenex in the U.S. and other countries. Strata-X and Giga are trademarks of Phenomenex, Inc. Strata-X is patented by Phenomenex 2010 Phenomenex, Inc. ll rights reserved. *Contact Phenomenex for more information. 11

12 R _I ustralia t: f: ustria t: f: elgium t: +31 (0) f: +31 (0) Canada t: (800) f: (310) Denmark t: f: Finland t: (09) f: France t: f: Germany t: f: Ireland t: f: Italy t: f: Luxembourg t: +31 (0) f: +31 (0) Mexico t: (55) f: (310) Netherlands t: f: New Zealand t: f: Norway t: f: Puerto Rico t: (800) 541-HPLC f: (310) United Kingdom t: f: ll other countries: Corporate ffice US t: (310) f: (310) Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex US, International Department at

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