Chromatography Bulk Media. Great Performance. with even Greater. Production Economy. Discovery to Production. Phenomenex is here to deliver

Transcription

1 Chromatography Bulk Media Great Performance with even Greater Production Economy Discovery to Production Phenomenex is here to deliver

2 Expect More From Your Purification Media Partner We know there s not just one characteristic that makes our products the purification media of choice. Chromatography media is complex and multidimensional, therefore, we scrutinize each batch of our prep / process chromatography packings to deliver maximum results with less downtime. Robust Yield Longevity Recovery Reliability Metal-free Surface Area Selectivity Throughput Cycle-time Pore size Delivery ReproducibilityLoad-per-cycle Purification Resolution Economics Chemical stability Symmetry Efficiency Confidence Support Loading Flow rate Mechanical strength Retention Particle size 03 Phenomenex, Inc. All rights reserved. Phenomenex l WEB:

3 Wide Range of Unique Selectivities and Services The separation characteristics of each Phenomenex media offer unique selectivity for each type of application and significant production gains for maximum loadability and process economy. Achieve identical performance from analytical development to large process scale. ur media is available in a wide range of particle sizes and designed for the exacting standards of process, pilot, and commercial scale-up. Services Information Screening, Method ptimization, Scale-up and More 6-7 Bulk Media Small Molecules Peptides Proteins Chiral Molecules ligonucleotides Capture and Concentrate Information rdering Proven Performance 8-34 Increased Loadability for Biomolecule Separations Unique Reversed Phase Chemistries for Complex Mixtures High ph Process Separations Polysaccharide Chiral Columns Dependable. Scalable. Affordable. Polysaccharide Supports with Excellent Enantioselectivity Purification of Synthetic ligonucleotides 8 34 Premium Low/ Medium Pressure Purifications 9 9 * For detailed phase information, review the media comparison guide on pages Interested in evaluating a new selectivity? See page 34 for details on our scout columns Phenomenex l WEB: 3

4 We have been very satisfied with the bulk media performance and the support we have received from Phenomenex. We trust Phenomenex as one of our key suppliers for GMP purification media. Bachem Inc., USA Phenomenex Corporate Headquarters Torrance, California, USA 4 Phenomenex l WEB:

5 Increase Yield, Purity, and Column Lifetime We have redefined the standard for industrial process media and control all areas of media production. We Specialize in Media with: High surface area for increased loadability Wide range of media for superior selectivities Superior mechanical strength for long column lifetimes Enhanced chemical stability for added versatility We GUARANTEE Media with: Controlled pore size diameter and volume Narrow particle size distribution Reproducible bonding Exacting scalability ptimized packing density Metal-free silica Phenomenex s quality management system is IS 900:008 certified. This certification validates that all our processes are fully established, functional, and meet international standards. Picture Courtesy of NovaSep Phenomenex l WEB: 5

6 PHENLGIX A New Era of Technical Support Services, Let Us Do the Work for You PhenoLogix, our in-house application support lab, saves you time and money by screening multiple scout columns and solvent strategies for new purification methods or revalidating your current methods. We work together to make you successful by minimizing your process purification development time and optimizing your purification method. Chiral Screening Normal Phase Reversed Phase Polar rganic SFC Method ptimization Services Fast Turnaround Easy Method Transfer Continued Support 3Preparative and Process Scale-Up Media Screening Small Scale Purification DAC Packing Assistance Jeff Layne, Ph.D. Technical Manager, Phenologix 6 Phenomenex l WEB:

7 PhenoLogix Your Method, ur Scientists PHENLGIX Quality Products, Advanced Performance, Complete Support For more information or to begin a project today, please contact your local Phenomenex representative or us at phenologix@phenomenex.com You can also visit us online: ur scientists at American Peptide have taken advantage of Phenomenex s column packing services, application development, and project-specific consultation services for some of our most challenging separations. American Peptide Company, USA Phenomenex l WEB: 7

8 LUNA Proven Performance Luna high surface area (00 Å; 400 m²/g) silica packing materials provide optimized parameters specifically designed for the purification of small molecules and peptides. This media allows high loading with excellent lifetimes. ptimized loading parameters include: High-surface area for increased loading Silica smoothness for stable packed beds ptimum pore size/distribution provide outstanding performance High pore volume offers increased surface area Fine tuned bonding density for excellent reproducibility Usually, these characteristics would result in a weak silica particle, however, the advanced silica technology behind Luna media yields a particle that is extremely uniform in its sphericity, surface smoothness, and overall physical structure. We use the Phenomenex Luna HPLC as our standard purification media to purify our customer s peptides. In addition to the excellent loadability and selectivity of the media itself, the Phenomenex PREP Team supports their entire line of products very effectively. Major Biotech Manufacturer, USA Did you know we offer FREE compound screening and purification services? Learn more about PhenoLogix on page 6 8 Phenomenex l WEB:

9 utstanding Lifetime LUNA To achieve maximum purification economy, each batch of Luna media begins with a uniform starting point, the base silica itself. Luna silica advantages: Very smooth and spherical particles Extremely stable bed packing Low particle shearing and breakage during packing Reproducible performance Exceptionally long column lifetimes Extensive Quality Testing for Increased Loading and Reproducibility We carefully control porosimetry (diameter and volume) for maximum column performance and loading capacity. We test all our media by nitrogen adsorption to ensure consistent quality results and guarantee that pore size distribution falls within tightly controlled limits. With Luna, expect increased: Mass transfer Available surface area Reproducibility We are using chromatography media from Phenomenex for GPL/ GMP purposes, therefore we audited Phenomenex USA as a manufacturer. From the beginning, we were impressed with Phenomenex and the attitude of their employees. Phenomenex is a unique company in many aspects. Their degree of dedication to customer service, to the organization of the QMS system and last but not least the positive atmosphere in the company is impressive. The outcome of the audit was to our fullest satisfaction. Major Generic Pharma Company, Europe Phenomenex l WEB: 9

10 LUNA Available for Multiple Campaigns Luna Preparative HPLC packings provide a better balance of both higher loadability and better mechanical stability than other high surface area media. Luna media offers: Multiple axial compression packings without shearing or breaking Longer column lifetimes Less frequent repacking SEM of Bulk Silica 00 Å - Packing Pressure: 40 Bar Before Packing LUNA 0 µm C8() 00 Å Leader in Economics The extremely low particle densities of Luna media require less material to pack a given volume, while maintaining high mass loading, resolution, and purity. This can have an enormous impact on long-term operating costs of production or process methods. After Packing in DAC System LUNA 0 µm C8() 00 Å Phenomenex Luna vs. Eka Chemicals Kromasil Bulk HPLC Media 5kg Luna 0 µm C8() 0kg 0kg 0kg 0kg = 85 kg 5 % less Luna media than a competitive media offering significant cost savings.* Kromasil 0 µm C8 0kg 0kg 0kg 0kg 0kg = 00 kg 50 L Column Volume * The packing density of each media was determined by packing 00 g into a 50 mm ID DAC column and measuring the resulting bed height. The packing density was calculated as the ratio of sorbent weight over packed bed volume. Calculated amount of media (g) required for packing a column with the dimensions L x ID (mm) by axial compression: (ID/0) x (3.4)(L/0)(packing density) Kromasil is a registered trademark of Eka Chemicals. Phenomenex is in no way affiliated with Eka Chemicals. 0 Phenomenex l WEB:

11 Dependable Chemical Stability LUNA Luna features an extended ph range of.5 to 0.0* for most of its phases due partly by the density of the bonded phase. Luna C8() columns are exhaustively bonded for great peak shape independent of ph, indicating free silanols are well shielded from analyte compounds. Chemical stability at ph levels outside the normal constraints of -7 is a critical factor in today s process environments, allowing: Greater loading capacity ptimization of sample solubility ph adjustment to optimize recovery of active pharmaceutical ingredients (API s) Column regeneration Wide range of buffer options High Chemical Stability from ph:.5 to 0.0* for over 0000 hours Excellent Performance at Low ph Luna 5 µm C8() 00 Å Extended Media Lifetime Even Under Caustic Washes Luna 5 µm C8() 00 Å Efficiency of Toluene pl/col t R,k, Symmetry of Toluene and Pyridine ph.5 ph Hours of Exposure Test Conditions: Column stability tested under highly acidic conditions. Continuous flush in 0. % TFA (ph.5) in Water/Acetonitrile, 50:50. Efficiency of Toluene pl/col t R,k, Symmetry of Toluene and Pyridine Hours of Exposure Test Conditions: Column stability tested under highly basic conditions. Continuous flush in 0 mm Na HP 4 (ph 0.0) in Water/Acetonitrile, 50:50. KEY: Efficiency, Plates for Toluene Symmetry of Toluene t R of Toluene (min) Symmetry of Pyridine k of Toluene KEY: Efficiency, Plates for Toluene Symmetry of Toluene t R of Toluene (min) Symmetry of Pyridine k of Toluene The bulk media products and product support services provided by Phenomenex are of consistently high quality. We use Phenomenex media in the cgmp manufacture of complex peptides for our customers. Almac, United Kingdom * ph range under isocratic conditions. ph range is under gradient conditions. Phenomenex l WEB:

12 LUNA Consistent Selectivity Across All Particle Sizes Luna offers a complete range of selectivities on 3 μm, 5 μm, 0 μm and 5 μm particle sizes. Each phase features identical technology and base silica across all particle sizes, making scale up from analytical through prep conditions quick and simple. mau Luna 5 µm C8() Which Luna media should you choose? 00 Luna 0 μm-prep offers excellent economy and great performance with narrow particle size distribution, suitable for most applications. Luna 0 μm is available when higher preparative performance is needed for difficult separations and offers extremely narrow particle size distribution producing higher efficiencies with increased yield. Need additional media selection assistance? Contact your local Phenomenex bulk media representative. 0 mau min Luna 0 µm C8() min mau 400 Luna 5 µm C8() min Phenomenex l WEB:

13 Increased Loadability for Biomolecule Separations JUPITER Jupiter 300 features ultra-pure metal-free silica with 300 Å pores, making it suitable for purifying target proteins and large peptide therapeutics. Its dense, bonded phase coverage decreases nonspecific interactions, leading to easier quantitation and improved resolution and separation of complex mixtures. This produces higher yields, fewer purification runs, and better overall economy. High mechanical-strength silica for better packing and longer lifetime Large loading capacity for higher sample recovery.5 to 0 ph stability for easy column cleaning and regeneration Low particle densities, requiring less material to pack columns Easy Scale-Up for Exacting Performance Jupiter uses identical bonding and base silica technology in both analytical and preparative materials. 5 μm, 0 μm and 5 μm Jupiter 300 Å media easily scales up with minimal changes to the separation. All Jupiter particle sizes offer: Resistance to silica shearing and fine formation at high packing pressures and flow rates Easy material cleaning and regeneration Phenomenex l WEB: 3

14 JUPITER High Mechanical Strength Longer Column Lifetimes After packing in a Dynamic Axial Compression (DAC) column, Jupiter silica maintains its smooth uniform structure, while other silicas show fines of crushed silica particles. This allows Jupiter to maintain excellent performance for longer periods. Jupiter bulk media offers: More stable column bed Lower backpressure Reduced fouling After close examination of the unpacked material by scanning electron microscopy, the strength of Jupiter 300 silica is clear. Jupiter particles remain structurally unchanged after the DAC packing. After DAC packing, Vydac particles turn into fines that can clog frits, dramatically increasing backpressure leading to decreased performance and shortened column / material lifetime. High Mechanical Strength Silica Resists Sheering Virgin Bulk Media Jupiter µm C8() 300 Å After Packing in DAC System Jupiter µm C8() 300 Å Jupiter 300 C4 is a silica based resin consisting of very stable particles and combines highest selectivity / resolution together with high capacity. Richter-Helm BioLogics [has] used Jupiter 300 C4 resin in three different preparative scales to date and recognized significant robustness and reproducibility regarding column packing quality, selectivity and capacity. Utilizing Jupiter 300 C4 resin in biopharmaceutical drug manufacturing enables a straight forward purification schedule saving facility time. Richter-Helm BioLogics Dengelsberg, Germany Virgin Bulk Media Vydac 0-5 µm 4TPC C4 After Packing in DAC System Vydac 0-5 µm 4TPC C4 Vydac is a registered trademark of Alltech Associates, Inc. Phenomenex is in no way affiliated with Alltech Associates. Comparison is not representative of all applications. 4 Phenomenex l WEB:

15 Engineered for Reproducibility and Quality JUPITER Jupiter silica particle consistency, size, and smoothness is tightly controlled for quality and reproducibility. ver 5 individual quality control tests performed on every batch of Jupiter material Every aspect of media reproducibility is specified, tested, and reported in a Materials Validation Document (MVD) ph.5-0 stability gives robust, method development opportunities for increased yield. Batch-to-Batch Reproducibility of Jupiter 300 Å 5 µm C8 Batch 6 Batch 5 Batch 4 Batch 3 Batch Batch Column: Jupiter 5 µm C8 300 Å Dimensions: 50 x 4.6 mm Part No.: 00G-4053-E0 Mobile Phase: A: 0. % TFA in Water B: 0. % TFA in Acetonitrile Gradient: A/B (75:5) to A/B (45:55) in 5 min Flow Rate:.0 ml/min Detection: 0 nm Sample:. Yeast Cytochrome c. Equine Cytochrome c 3. Bovine Cytochrome c Scale-Up Quickly Between Particle Sizes 5 µm C8 0 µm C8 5 µm C App ID 6659 App ID 6660 App ID Phenomenex l WEB: 5

16 JUPITER Extended Chemical Stability from ph Jupiter 300 has been tested and is stable from ph.5 to 0 for 6 4 over,500 hours. Jupiter offers increased column lifetime at extreme ph levels and method development opportunities for in- 0 0 creased yield Stability of Jupiter 300 C8 at ph.5 and ph 0.0 ph Hours of Exposure Test Conditions: Column flushed in 0 mm Na HP 4 (ph 0.0) in Water/Acetonitrile (50:50) Hours of Exposure Test Conditions: Column flushed in 0. % TFA (ph.5) in Water/Acetonitrile (50:50) KEY: Efficiency, Plates for Toluene Asymmetry of Toluene t R of Toluene (min) Asymmetry of of Pyridine k of Toluene KEY: Efficiency, Plates for Toluene Asymmetry of Toluene t R of Toluene (min) Asymmetry of of Pyridine k of Toluene Utilize ph for Method Development of Protein Separations ph.0 Bradykinin Neurotensin Eledoisin Bombesin ph 4.0 Bradykinin Bombesin Neurotensin Eledoisin Eledoisin ph 9.5 Bradykinin Neurotensin Bombesin App ID min 6 Phenomenex l WEB:

17 Less Material Required JUPITER Jupiter 300 media have low particle densities, requiring less material to pack a given column volume. While less media is needed to pack a given dimension compared to other common prep sorbents, mass loading remains high with peak resolution and purity maintained. Enormous Impact on Long-term perating Costs Example: Packing a 50 L column volume would require up to 34 % less Jupiter 300 Å media than a competitive media offering significant cost savings.* Phenomenex Jupiter 300 vs. Alltech Vydac Bulk HPLC Media Jupiter 0 µm 300 C4 0kg 0kg 4kg = 54 kg 34% Media Saving* Vydac 0 µm 300 C4 0kg 0kg 0kg 0kg kg = 8 kg 50 L Column Volume Have you heard of PhenoLogix s FREE protein and peptide screening services? Method Development and ptimization Method Re-Validation Small Scale Purification Learn more on page: 6 * The packing density of each media was determined by packing 00 g into a 50 mm ID DAC column and measuring the resulting bed height. The packing density was calculated as the ratio of sorbent weight over packed bed volume. Calculated amount of media (g) required for packing a column with the dimensions L x ID (mm) by axial compression: (ID/0) x (3.4)(L/0)(packing density) Vydac is a registered trademark of Alltech Associates, Inc. Phenomenex is in no way affiliated with Alltech Associates. Phenomenex l WEB: 7

18 SYNERGI Unique Reversed Phase Chemistries for Complex Mixtures Successfully separate complex mixtures of highly polar and/or non-polar compounds and challenging analytes with the unique selectivity range of Synergi high surface area purification media, significantly increasing purification yield. Synergi is available in four unique phases, each offering dramatic differences in: Selectivity Retention time Resolution Synergi Polar-RP Phenyl Ether-Linked For polar and aromatic mixtures Synergi Fusion-RP C8 Polar Embedded Balanced non-polar and polar performance Ether linkage increases aromaticity of the phenyl group and also provides π-π interactions with conjugated compounds Aq Aq Polar endcapping provides added retention for polar compounds Embedded polar group complements C8 ligand with balanced polar selectivity TMS Aq Aq TMS TMS endcapping ensures sharp peaks Ultra-pure Silica Ultra-pure Silica Synergi Hydro-RP C8 Polar Endcapped Strong non-polar and polar retention Synergi Max-RP C TMS Endcapped Excellent for basic compounds at neutral ph Polar endcapping provides added retention for polar compounds High density ligands and extensive endcapping ensure sharp peaks Aq Aq TMS TMS Ultra-pure Silica Ultra-pure Silica 8 Phenomenex l WEB:

19 Increased Loading with Unique Selectivities SYNERGI Each Synergi phase has a unique selectivity profile composed of multiple characteristics. These differences offer a complementary selectivity to the standard C8, C8, or silica phases traditionally employed in larger scale HPLC. Phases Description Selectivity Profile Synergi Polar-RP (00 % Aqueous Stable) This ether linked phenyl column is polar endcapped and offers high cation retention capabilities to improve retention for ionized bases. Synergi Polar-RP Hydrophobicity Polarity H-Bonding Aromatic Selectivity Low High USP:L Silanol Activity (ph.5) Synergi Fusion-RP (00 % Aqueous Stable) A low ligand density polar embedded C8, this unique phase contributes to hydrogen bonding and donating. It provides balanced selectivity for acids and bases. Synergi Fusion-RP Hydrophobicity Polarity H-Bonding Aromatic Selectivity Low High LC/MS Certified USP:L Silanol Activity (ph.5) Synergi Hydro-RP (00 % Aqueous Stable) Polar endcapped C8 column that provides very high hydrophobic interactions and hydrogen donating capabilities make this column ideal for retaining polar bases. Synergi Hydro-RP Hydrophobicity Polarity H-Bonding Aromatic Selectivity Low High USP:L Silanol Activity (ph.5) Synergi Max-RP Densely bonded C contributes a lot of hydrophobic retention and steric based selectivity. Combined characteristics of the base silica and the bonded phase will also provide hydrogen bonding benefits. Synergi Max-RP Hydrophobicity Polarity H-Bonding Aromatic Selectivity Low High LC/MS Certified Silanol Activity (ph.5) We regularly use RP stationary phases from Phenomenex for our separation problems. Especially Synergi Polar-RP was found to often show the desired selectivity, distinguishing this phase from other RP phases. CARBGEN AMCIS, Switzerland Phenomenex l WEB: 9

20 SYNERGI Selectivity Like No ther ffering a balanced combination of hydrophobic and polar selectivity, Synergi Fusion-RP separates compounds exhibiting moderately polar and hydrophobic characteristics. Hydrophobic Basic Compounds The slightest variations in compound polarity and aromaticity are exploited by Synergi Polar-RP to achieve the greatest separation between polar and/or aromatic compounds. Increased resolution of polar compounds with Synergi Polar-RP compared to traditional C8 phases Balanced polar and hydrophobic retention allows for superior selectivity Increase retention and separation of earlier eluting polar compounds with additional polar selectivity Longer Polar Retention Columns: Synergi 4 µm Fusion-RP Typical C8 Dimensions: 50 x 4.6 mm Mobile Phase: 0 mm Potassium Phosphate, ph.5 / Acetonitrile (75:5) Flow Rate:.0 ml/ min Detection: 0 nm Sample:. Maleic acid. Chlorpheniramine 3. Triprolidine 4. Diphenhydramine 4 Less Hydrophobic Retention Synergi 4 µm Fusion-RP 4 App ID 4840 min Typical C8 min 3 3 Synergi 4 µm Polar-RP Improved Selectivity! Waters 5 µm SymmetryShield RPC8* 6 6 App ID 485 App ID 55 Waters 5 µm XTerra RP8* App ID 56 Waters 5 µm Symmetry C8* App ID min Did you know we offer FREE compound screening services? Learn more about PhenoLogix on page 6 Columns: Synergi 4 µm Polar-RP Waters 5 µm SymmetryShield RPC8 Waters 5 µm Symmetry C8 Waters 5 µm XTerra RP8 Dimensions: 50 x 4.6 mm Mobile Phase: 0 mm Potassium phosphate ph 3 / Methanol (50:50) Flow Rate:.0 ml/ min Detection: 30 nm Temperature: Ambient Injection: μl Sample:. Metaproterenol (0.4 μg). Pindolol (0.6 μg) 3. Metoprolol (0.5 μg) 4. Alprenolol (0.3 μg) 5. Propranolol (0.04 μg) 6. Ethylparaben (0.4 μg) *Comparative separations may not be representative of all applications. SymmetryShield is a trademark of Waters Corp. XTerra and Symmetry are registered trademarks of Waters Corp. Phenomenex has no affiliation with Waters Corp. Columns used for comparison studies were manufactured by and purchased from Waters Corp. 0 Phenomenex l WEB:

21 High ph Process Separations GEMINI Gemini features a ph stability from -, making it optimal for high alkaline washes and high ph purifications of basic drugs. ptimized parameters include: Innovative surface layer for increased ph stability High-surface area for increased loading Silica smoothness for stable packing beds Bonding density for excellent reproducibility H H H R H Si R R Si H Si R H Si H R H R R R Si Si H R H Si R R Si R R Si Si H H R H Si Si Si R Si H Si Si Si R R R R Si R R Si Si Si Si Si Si Si R Si R R R R Si Si Si R Si Si R Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si TWIN (Two-In-ne) Technology is what gives Gemini media its superior performance edge. During the final stage of silica manufacturing, a unique silica-organic layer is grafted to create a completely new composite particle. Since the internal base silica is unaltered by this manufacturing process the particle retains the mechanical strength and rigidity of silica. This provides excellent efficiency, while the silica-organic shell protects the particle from chemical attack. Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si Si How It Works Gemini rganosilane Coat Resists High ph Attack Standard Silica Silica Dissolution Multi-Point Ligand Attachment Resists Low ph Ligand Cleavage Ligand Cleavage H + H + H + Si Multi - Point Attachment H Si H + H 3 C Si H + Si CH 3 H Si H + Phenomenex l WEB:

22 GEMINI Increase Sample Load by -3X by Adjusting ph The advent of ph stable (-) process media, such as Gemini C8, enables improved retention and resolution of basic compounds at high ph without compromising column lifetime. Modifying ph conditions can increase selectivity allowing for larger yields. ph-lc - Extended ph - Stability for Alternate Selectivity ph.5.0 % TFA in Water ph mm Ammonium Formate APP ID 5457 APP ID ,3,5 4,3 5 Poor Resolution for Basic Compounds min min ph mm Phosphate Buffer,3 APP ID min Phenomenex l WEB:

23 Greater Economy for High ph Process Separations GEMINI Gemini process media offers several advantages over conventional process media that are critical factors in today s process environments. Allows Clean-in-Place (CIP) processes by means of a caustic wash No leaching of bonded phase or silica matrix that can contaminate your product Longer overall lifetime at extended ph conditions Prevents chromatographic changes (retention, selectivity, peak shape, etc.) 00 times increase in stability* 0 ph mm Ammonium Bicarbonate Buffer ph.0 0 mm Triethylamine Buffer 546 APP ID 5460 APP ID Great resolution for Basic compounds min min Column: Gemini 5 µm C8 Dimension: 50 x 4.6 mm Part No.: 00F-4435-E0 Mobile Phase: Acetonitrile/Buffers at various ph s (see chromatograms), (50:50) Flow Rate:.0 ml/min Temperature: C Detection: 54 nm Sample:. Chlorpropamide (pka 5.0). Butylparaben 3. Lidocaine (pk a 7.9) 4. Triprolidine (pk a 6.5) 5. Dextromethorphan (pk a 9.) * Compared to a traditional column with a ph -0 range. Phenomenex l WEB: 3

24 LUX Complete Chiral Solutions Polysaccharide Chiral Columns Dependable. Scalable. Affordable. Achieving optimal chiral separation is easier than ever with four unique bulk Lux polysaccharide stationary phases to screen. Choose a phase, then transfer the method to process, pilot, and commercial scale. Lux chiral columns and bulk media simplify the separation process: Unique and traditional phases that increase the success rate of the chiral screen Consistent particle size distribution so performance is maintained Mechanically strong media for increased stability Available in multiple particle sizes for direct scale up (0 μm and 0 μm bulk media for process scale purifications; 3 μm and 5 μm packed columns for screening and small scale purifications) Resolve Your Enantiomers with Four Unique Phases* The Lux family of bulk cellulose chiral selectors provides a variety of complementary selectivities. Lux Cellulose- Cellulose tris(3,5-dimethylphenylcarbamate) H H C H Cellulose--CNH Lux Cellulose- Cellulose tris(3-chloro-4-methylphenylcarbamate) H H C H H C Cellulose--CNH H H Cl Screen for the most effective chiral separation under the following conditions: Reversed Phase Polar rganic Normal Phase Supercritical Fluid Chromatography (SFC) Lux Cellulose-3 Cellulose tris(4-methylbenzoate) H H C H Have you heard of PhenoLogix s FREE chiral screening services Method Development and ptimization Method Re-Validation Small Scale Purification Learn more on page: 6 Cellulose- Lux Cellulose-4 Cellulose tris(4-chloro-3-methylphenylcarbamate) H H C H Cl Cellulose--CNH * Based on 33 compounds screened on all Lux phases 4 Phenomenex l WEB:

25 Versatile Polysaccharide Chiral Phases for HPLC, SMB, and SFC Polysaccharide Chiral Columns Dependable. Scalable. Affordable. LUX Utilizing differences in selectivity can help develop methods more efficiently by offering broad and contrasting chiral recognition abilities. Lux chiral selectors provide an opportunity for increased yield. Etozolin Lux Cellulose- a = min Lux Cellulose- a =.48 App ID 948 App ID 947 Lux Cellulose-3 a = min Lux Cellulose-4 a =.35 App ID 95 App ID min min Conditions for all columns: Dimensions: 50 x 4.6 mm Mobile Phase: Acetonitrile / 0. % Diethylamine in 0 mm NH 4 HC 3 (60:40) Flow Rate: ml/min Detection: 0 nm Temperature: Ambient N S N ptimal Resolution Based on a four phase screen under Reversed Phase conditions, the optimal chiral stationary phase for resolving Etozolin is Lux Cellulose-3. Lux Cellulose-3 a =.70 App ID min Comparative separations may not be representative of all applications. Phenomenex l WEB: 5

26 LUX Supercritical Fluid Chromatography Polysaccharide Chiral Columns Dependable. Scalable. Affordable. Baseline Separation of Enantiomers UV-VIS Polarimeter Lux Cellulose- offers great peak shape at 0 nm App ID 8865 Dimensions: 50 x 4.6 mm Flow Rate:.5 ml/min Detection: 0 nm Load: 300 µg 0 µl Analytical and Axia packed columns have been extensively tested on various SFC systems and all column ID s and lengths are SFC compatible. Terfenadine UV-VIS 5x Load Increase verloading study with increased analytical load showing impurities eluting after major enantiomers only detected at 54 nm min Polarimeter App ID 8866 Dimensions: 50 x 4.6 mm Flow Rate:.5 ml/min Detection: 54 nm Load:.5 mg in 50 µl Conditions for all columns: Columns: Lux 5 µm Cellulose- Mobile Phase: Methanol with 0. % DEA/ Carbon Dioxide (5:75) Column Temperature: 35 C Polarimeter: ALP-PDR-Chiral Sample: Terfenadine with ethanol dissolution solvent 70x Load Increase High loading capacity media along with stacking injections allow for increased yields and productivity Closer stacked injections can not be used due to the impurities eluting after the major enantiomers App ID 8867 Dimensions: 50 x. mm Flow Rate: 50 ml/min Detection: 0 nm Load: 05 mg in 3.5 ml 7.5 cycles per hr/ 787 mg per hr 6 Phenomenex l WEB:

27 Maintain Performance at Any Scale Polysaccharide Chiral Columns Dependable. Scalable. Affordable. LUX High Efficiency trans-stilbene xide from Analytical to Preparative mm ID N = 70,664 a = min App ID 883 Conditions for all separations except where noted: Column: Lux 5 µm Cellulose- Dimensions:. 50 x 4.6 mm. 50 x 50 mm Mobile Phase: Hexane/Isopropanol (90:0) Flow Rates:. 0.5 ml/min. 50 ml/min Detection: 0 nm Temperature: Ambient Injections:. µl. 30 µl mm ID N = 75,56 a =.9 App ID 885 Same efficiency as analytical column min Want to see how Axia pre-packed columns outlast and outperform all others? See page 3. Phenomenex l WEB: 7

28 CLARITY Purification of Synthetic ligonucleotides Clarity ligo-rp Unique media specifically designed for the reversed phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity. The media is based on composite particle TWIN technology that provides improved selectivity and efficiency for oligonucleotides when compared to competing hybrid, polymer, and silica media. RP-HPLC Preparative Purification Easily separate N- failure sequences from target oligo with > 90 % purities Purify oligos up to 60 nt in length Trityl-off purification of DNA, RNA, thioates, and modified/labeled oligonucleotides 3 µm, 5 µm, 0 µm particles for seamless scaling Preparative 0nt DNA ligo-rp Purification mau nt 0nt Column: Clarity 3 µm ligo-rp C8 Dimensions: 50 x 0 mm Part No.: 00B-444-N0 Mobile Phase: A: 50 mm TEAA ph 7.5/ 5 % Acetonitrile B: Methanol Gradient: 0 % to 60 % B in 0 minutes Flow Rate: 5 ml /min Detection: 60 nm Sample: 0nt DNA min App ID 5947 Clarity ligo-wax Clarity ligo-wax is a crosslinked weak anion exchanger media designed for successful ion-exchange purification of synthetic DNA/RNA. ligo-wax is an advantageous combination of purity, capacity, mechanical strength, cost, and efficiency. Excellent efficiency column results in > 90 % purities due to good fractionation of closely eluting compounds High loading capacity due to very high density ligand Increase productivity by running at higher flow rates and pressures Purify Failure Sequences and Contaminants from Target Sequence Ion-exchange is an excellent separation mode for purifying contaminants and failure sequences from target sequences. Clarity ligo-wax, due to its increased efficiency compared to other ionexchange columns, has the ability to recognize minute charge differences in nucleotide sequences such as failure sequences or base substitutions. DNA Purification of N- Sequence from Target N Sequence mau nt 0nt min Column: Clarity 0 μm ligo-wax Dimensions: 50 x 4.6 mm Part No.: 00F-445-E0 Mobile Phase: A: Water B: Acetonitrile C: 00 mm Tris, ph 8 D: M NaCl Gradient: A/B/C/D (70:0:0:0) to (0:0:0:60) in 0 min Flow Rate:. ml/min Detection: UV-Vis Abs.-Diode Array (ambient) Sample: Depurinated A & G and 0mer DNA App ID

29 Premium Low/Medium Pressure Purifications SEPRA rdering Information Polymer Sepra-ZT Small Pore Polymer Resin Polymer Sepra-ZTL Large Pore Polymer Resin Silica Sepra 00 % Silica Resin Phase Sepra ZT (30 µm, 85 Å) Sepra ZT-SCX (30 µm, 85 Å) Sepra ZT-WCX (30 µm, 85 Å) Sepra ZT-SAX (30 µm, 85 Å) Sepra ZT-WAX (30 µm, 85 Å) Sepra ZTL (5 µm, 330 Å) Sepra ZTL-SCX (5 µm, 330 Å) Sepra ZTL-WCX (5 µm, 330 Å) Sepra ZTL-SAX (5 µm, 330 Å) Sepra ZTL-WAX (5 µm, 330 Å) Sepra C8-E (50 µm, 65 Å) Sepra C8-T (50 µm, 35 Å) Sepra C8 (50 µm, 65 Å) Sepra Phenyl (50 µm, 65 Å) Sepra CN (50 µm, 65 Å) Sepra NH (50 µm, 65 Å) Sepra Florisil (70 µm, 80 Å) Sepra SCX (50 µm, 65 Å) Sepra SAX (50 µm, 65 Å) Sepra WCX (55 µm, 70 Å) Sepra Silica (50 µm, 65 Å) Sepra SDB-L (95 µm, 55 Å) Sepra EPH (00 µm, 70 Å) Phase Description MW Range Common Applications 00 g kg 0 kg Pyrrolidone modified styrenedivinylbenzene polymer Sulfonic acid modified styrenedivinylbenzene polymer Carboxylic acid modified styrenedivinylbenzene polymer Quaternary amine modified styrenedivinylbenzene polymer Primary, secondary amine modified styrenedivinylbenzene polymer Large particle, large pore pyrrolidone modified styrenedivinylbenzene polymer Large particle, large pore sulfonic acid modified styrenedivinylbenzene polymer Large particle, large pore carboxylic acid modified styrenedivinylbenzene polymer Large particle, large pore quaternary amine modified styrenedivinylbenzene polymer Large particle, large pore primary, secondary amine modified styrenedivinylbenzene polymer Endcapped silica-based C8 Wide pore endcapped silica-based C8 Endcapped silica-based C8 Endcapped silica-based phenyl Unendcapped silica-based cyano Unendcapped silica-based primary amine Magnesium silicate Pesticide Residue Grade Florisil Silica-based sulfonic acid Silica-based quaternary amine Silica-based carboxylic acid 0 kda 0 kda 0 kda 0 kda 0 kda 75 kda 75 kda 75 kda 75 kda 75 kda 0 kda 45 kda 0 kda 0 kda 0 kda 0 kda Reversed phase, hydrophobic, polar or aromatic, small molecule selectivity from aqueous samples in ph -4 including peptides and small proteins Strong ion-exchange of cationic or aromatic, small molecule selectivity from aqueous samples in ph -4 including peptides and small proteins Weak ion-exchange of cationic or aromatic, small - large molecule selectivity from aqueous samples in ph -4 including peptides and small large proteins Strong ion-exchange of anionic or aromatic, small molecule selectivity from aqueous samples in ph -4 including peptides and small proteins Weak ion-exchange of anionic or aromatic, small molecule selectivity from aqueous samples in ph -4 including peptides and small proteins Reversed phase, hydrophobic, polar or aromatic, small - large molecule selectivity from aqueous samples in ph -4 including peptides and small large proteins Strong ion-exchange of cationic or aromatic, small - large molecule selectivity from aqueous samples in ph -4 including peptides and small - large proteins Weak ion-exchange of cationic or aromatic, small - large molecule selectivity from aqueous samples in ph -4 including peptides and small large proteins Strong ion-exchange of anionic or aromatic, small - large molecule selectivity from aqueous samples in ph -4 including peptides and small large proteins Weak ion-exchange of anionic or aromatic, small - large molecule selectivity from aqueous samples in ph -4 including peptides and small large proteins Reversed phase, hydrophobic, small molecule selectivity from aqueous samples Reversed phase, hydrophobic, small-medium molecule selectivity from aqueous samples, including peptides and small proteins Reversed phase, hydrophobic, small molecule selectivity from aqueous samples Reversed phase, hydrophobic and aromatic, small molecule selectivity from aqueous samples Reversed or normal phase, pi electron/ aromatic, small molecule selectivity from aqueous or organic samples Reversed or normal phase, anion or polar, small molecule selectivity from aqueous or organic samples 04G K M G K-4466 Inquire 04G K-4478 Inquire 04G K-4485 Inquire 04G-4463 Inquire Inquire 04G K-4470 Inquire 04G K-4467 Inquire Inquire Inquire Inquire Inquire Inquire Inquire 04G K-4494 Inquire 04G K M G K M G K-4406 Inquire 04G K-4407 Inquire 04G K-4409 Inquire 04G K M kda Normal phase, polar, small molecule selectivity from organic samples 04G-44 04K-44 Inquire 0 kda 0 kda 0 kda Strong ion-exchange of cationic small molecules from aqueous or organic samples including peptides and small proteins Strong ion-exchange of anionic small molecules from aqueous or organic samples Weak ion-exchange of cationic small molecules from aqueous or organic samples including peptides and small proteins 04G K-443 Inquire 04G K-444 Inquire 04G-S07 04K-S07 Inquire Unendcapped silica 0 kda Normal phase, polar, small molecule selectivity from organic samples 04G K-440 Inquire Large particle, specialty normal phase silica Styrenedivinylbenzene polymer 0 kda Specialty resin for extractable petroleum hydrocarbon analysis 04G K-4508 Inquire 75 kda Reversed phase, hydrophobic or aromatic, small large molecule selectivity from aqueous samples in ph -4 including peptides and small - large proteins 04G-44 04K-44 Inquire 9

30 AXIA Novel Pre-packed Preparative Columns As a professional in preparative chromatography, you understand that proper scale-up development can lead to increased production yields. The best way to guarantee scalability of your purification is to use a scout column that mimics the performance of your large preparative DAC (Dynamic Axial Compression) column. The Axia patented packing technology (available in., 30 and 50 mm ID) is completely automated and monitored by multiple sensors allowing measurement and recording of all process parameters for every column. The result is an improved, more reproducible packing process that offers efficiencies and peak symmetries on par with DAC purification. Excellent Performance Excellent reproducibility Higher efficiency ver 0 unique selectivities in bulk media particle sizes SecurityGuard PREP available for longer column lifetimes Axia Packing Advantage Packing Piston The packing piston head is integrated into the column and locked by the piston retainers, so the pressure is never released. Media packed under ideal pressure Media is never allowed to relax, eliminating voids and dramatically improving reproducibility, column-to-column. Visit: to view a packing demonstration. 30 Phenomenex l WEB:

31 Unmatched Column Reproducibility AXIA The Axia packing process provides optimum bed densities that can be consistently reproduced column-to-column. This directly translates into consistent efficiency and peak asymmetry measurements and decreases the column variability seen in traditionally packed preparative columns. 0,000 Reproducible Column-to-Column Efficiency Average Efficiency (N) with Synergi 4 µm Hydro-RP 00 x. mm % RSD Reproducible Column-to-Column Peak Asymmetry Average Peak Asymmetry with Gemini 5 µm C8 50 x. mm % RSD 6 Density Comparison of Packed Beds Efficiency (N) 00,000 80, % Improved Avg. Efficiency Peak Asymmetry % Improved Avg. Peak Shape 5. Density (%) % Increase in Packing Density and More Uniformly Packed Bed 60, ,000 Conventional Slurry Packing Axia Packed Hydraulic Piston Compression Packing Conventional Slurry Packing Axia Packed Hydraulic Piston Compression Packing 53 Conventional Slurry Packing Axia Packed Hydraulic Piston Compression Packing Extremely long lifetime (8000 injections) Columns show very good efficiency No significant changes concerning the backpressure So far there is no comparable column for this application like the Luna-Axia C8(), 5 µm in 50 x. Bayer Schering Pharma, Wuppertal Germany Phenomenex l WEB: 3

32 Media Comparison Guide Phenomenex media offers identical performance from analytical to large process scale, and is available in a wide range of particle sizes, all supported in over 70 countries. Proven Lifetime and Performance Luna High surface area (00 Å; 400 m /g) for maximum sample loading and resolution of closely eluting peaks Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Luna C8 (3) 0-Prep * Luna C8 () 3, 5, 0, 0-Prep, * Luna C8() 3, 5, 0, 0-Prep, * Luna C4() 0-Prep * Luna Phenyl- Hexyl 3, 5, 0, 0-Prep, * Luna Silica (3) 0-Prep Luna Silica () 3, 5, 0, 0-Prep, Luna CN 3, 5, Luna NH 3, 5, Luna SCX 5, Small Molecules Peptides Proteins Chiral Applications Type of Compounds Loading Page Neutrals 8- ligonucleotides Capture and Concentrate Acids Polar Hydrophobic Bases Available Surface Area *ph range is.5-0 under isocratic conditions and under gradient condtions. Increased Loadability for Biomolecule Separations Jupiter Jupiter 300 Å - Provides excellent resolution between proteins with similar properties. ptimal for the separation of intact proteins > 0,000 MW. Jupiter Proteo - Resolves Peptides and Small Proteins < 0, 000 MW Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Jupiter C8 5, 0, Jupiter C4 5, 0, Jupiter Proteo 4, Small Molecules Peptides Proteins Chiral Applications Type of Compounds Loading Page Neutrals 3-7 ligonucleotides Capture and Concentrate Acids Polar Bases Available Surface Area Unique Reversed Phase Chemistries for Complex Mixtures Synergi Alternative selectivity to C8 that offers increased retention of small polar and/or aromatic compounds in HPLC and SFC Packing Material Synergi Fusion-RP Synergi Max-RP Synergi Hydro-RP Synergi Polar-RP Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range 4, * 4, * 4, , *ph range is.5-0 under isocratic conditions and under gradient condtions. Small Molecules Peptides Proteins Chiral Applications Type of Compounds Loading Page Neutrals 8-0 Hydrophobic ligonucleotides Capture and Concentrate Acids Polar Hydrophobic Bases Available Surface Area 3 Phenomenex l WEB:

33 High ph Process Separations Gemini ph stable (-) media optimal for high alkaline washes and high ph purifications of basic drugs Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Small Molecules Peptides Proteins Chiral Applications Type of Compounds Loading Page Neutrals -3 ligonucleotides Capture and Concentrate Acids Polar Bases Available Surface Area Gemini C8 3, 5, Polysaccharide Supports with Excellent Enantioselectivity Lux Unique polysaccharide chiral phases for HPLC, SMB, and SFC Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Small Molecules Peptides Proteins Chiral Applications Type of Compounds Loading Page Neutrals 4-7 Hydrophobic ligonucleotides Capture and Concentrate Acids Polar Bases Available Surface Area Lux Cellulose- 5, 0, 0, Lux Cellulose- 5, 0, 0, Lux Cellulose-3 5, 0, Lux Cellulose-4 5, 0, Purification and Analysis of Synthetic ligonucleotides Clarity ligo-rp and Clarity ligo-wax RP-HPLC purification of failure from target sequences Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Small Molecules Peptides Proteins Chiral Applications Type of Compounds Loading Page Neutrals 8 Hydrophobic ligonucleotides Capture and Concentrate Acids Polar Bases Available Surface Area ligo-rp 3, 5, ligo-wax Premium Low/Medium Pressure Purifications Sepra Capture and Concentrate Compounds of Interest Packing Material Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range Small Molecules Peptides Proteins Chiral Applications Type of Compounds Loading Page Neutrals 9 Hydrophobic ligonucleotides Capture and Concentrate Acids Polar Hydrophobic Bases Available Surface Area Sepra See page 9 See page 9 Key Best Suited Very Good Phenomenex l WEB: 33

34 rdering Information Scout Columns Achiral Media Luna (00 Å) Phases 50 x x 0 0 μm Inquire Inquire C8() 00G-453-E0 00G-453-N0 C8() 00G-450-E0 00G-450-N0 Phenyl-Hexyl 00G-485-E0 00G-485-N0 Silica() 00G-409-E0 00G-409-N0 CN 00G-4300-E0 00G-4300-N0 NH 00G-4379-E0 00G-4379-N0 SCX 00G-440-E0 00G-440-N0 0 μm-prep Inquire Inquire C8(3) 00G-466-E0 00G-466-N0 C8() 00G-434-E0 00G-434-N0 C8() 00G-433-E0 00G-433-N0 C4() 00G-4460-E0 00G-4460-N0 Phenyl-Hexyl 00G-435-E0 00G-435-N0 Silica(3) 00G-467-E0 00G-467-N0 Silica() 00G-43-E0 00G-43-N0 5 μm Inquire Inquire C8() 00G-473-E0 00G-473-N0 C8() 00G-47-E0 00G-47-N0 Phenyl-Hexyl 00G-486-E0 00G-486-N0 Silica() 00G-47-E0 00G-47-N0 Bulk Media Achiral Media Luna (00 Å) Phases 00 g kg 5 kg 0 kg 50 kg 00 kg 0 μm Inquire Inquire Inquire Inquire Inquire Inquire C8() 04G K L M N P-453 C8() 04G K L M N P-450 Phenyl-Hexyl 04G K L M N P-485 Silica() 04G K L M N P-409 CN 04G K L M N P-4300 NH 04G K L M N P-4379 SCX 04G K L M N P μm-prep Inquire Inquire Inquire Inquire Inquire Inquire C8(3) 04G K L M N P-466 C8() 04G K L M N P-434 C8() 04G K L M N P-433 C4() 04G K L M N P-4460 Phenyl-Hexyl 04G K L M N P-435 Silica(3) 04G K L M N P-467 Silica() 04G-43 04K-43 04L-43 04M-43 04N-43 04P-43 5 μm Inquire Inquire Inquire Inquire Inquire Inquire C8() 04G K L M N P-473 C8() 04G-47 04K-47 04L-47 04M-47 04N-47 04P-47 Phenyl-Hexyl 04G K L M N P-486 Silica() 04G-47 04K-47 04L-47 04M-47 04N-47 04P-47 Jupiter (300 Å and 90 Å) Phases 50 x x 0 0 μm Inquire Inquire 300 Å C8 00G-4055-E0 00G-4055-N0 300 Å C4 00G-468-E0 00G-468-N0 Proteo 90 Å 00G-4397-E0 00G-4397-N0 5 μm Inquire Inquire 300 Å C8 00G-4057-E0 00G-4057-N0 300 Å C4 00G-469-E0 00G-469-N0 Synergi (80 Å) Phases 50 x x 0 0 μm Inquire Inquire Fusion-RP 00G-445-E0 00G-445-N0 Max-RP 00G-4350-E0 00G-4350-N0 Hydro-RP 00G-4376-E0 00G-4376-N0 Polar-RP 00G-435-E0 00G-435-N0 Gemini (0 Å) Phases 50 x x 0 0 μm Inquire Inquire C8 00G-4436-E0 00G-4436-N0 Clarity Phases 50 x x 0 0 μm Inquire Inquire Clarity ligo-rp 00G-4445-E0 00G-4445-N0 Clarity ligo-wax 00G-445-E0 00G-445-N0 Jupiter (300 Å and 90 Å) Phases 00 g kg 5 kg 0 kg 50 kg 00 kg 0 μm Inquire Inquire Inquire Inquire Inquire Inquire 300 Å C8 04G K L M N P Å C4 04G K L M N P-468 Proteo 90 Å 04G K L M N P μm Inquire Inquire Inquire Inquire Inquire Inquire 300 Å C8 04G K L M N P Å C4 04G K L M N P-469 Synergi (80 Å) Phases 00 g kg 5 kg 0 kg 50 kg 00 kg 0 μm Inquire Inquire Inquire Inquire Inquire Inquire Fusion-RP 04G K L M N P-445 Max-RP 04G K L M N P-4350 Hydro-RP 04G K L M N P-4376 Polar-RP 04G K L M N P-435 Gemini (0 Å) Phases 00 g kg 5 kg 0 kg 0 μm Inquire Inquire Inquire Inquire C8 04G K L M-4436 Clarity Phases 00 g kg 5 kg 0 kg 0 μm Inquire Inquire Inquire Inquire Clarity ligo-rp 04G K L M-4445 Clarity ligo-wax 04G K L M-445 Chiral Media Lux (000 Å) Phases 50 x x 0 0 μm Inquire Inquire Lux Cellulose- 00G-450-E0 00G-450-N0 Lux Cellulose- 00G-450-E0 00G-450-N0 0 μm Inquire Inquire Lux Cellulose- 00G-4473-E0 00G-4473-N0 Lux Cellulose- 00G-4464-E0 00G-4464-N0 Lux Cellulose-3 00G-4504-E0 00G-4504-N0 Lux Cellulose-4 00G-4503-E0 00G-4503-N0 Additional scout columns available. Contact us for 3 µm, 4 µm, and 5 µm media scout columns. Chiral Media Lux (000 Å) Phases 0 g 00 g kg 5 kg 0 kg 0 μm Inquire Inquire Inquire Inquire Inquire Lux Cellulose- 04D G K L M-450 Lux Cellulose- 04D G K L M μm Inquire Inquire Inquire Inquire Inquire Lux Cellulose- 04D G K L M-4473 Lux Cellulose- 04D G K L M-4464 Lux Cellulose-3 04D G K L M-4504 Lux Cellulose-4 04D G K L M Phenomenex l WEB:

35 Trust, Reliability, Performance We are ready to partner with you Technology advances through the synergy of customer experience, feedback and vendor action. Through our close relationships in the marketplace, we are well placed to handle your immediate needs today while leading innovation to meet your needs tomorrow. Phenomenex Bulk Media Innovations Team If you would like to place an order, try a scout column, have questions on media selection, our would like to improve your purification work, please contact us anytime. ur dedicated Prep team is willing and able to support your purchase and offer purification yields like nothing you ve seen before. Prep@phenomenex.com WEB: Phenomenex l WEB: 35