Regis Technologies Ion Pair Reagents and Buffers
|
|
- Randell Dennis
- 5 years ago
- Views:
Transcription
1 egis Technologies Ion Pair eagents and Buffers Ion Pair HPLC greatly extends the applicability of HPLC by making the efficient octadecylsilyl columns amendable to use with ionized or ionizable samples. egis S-Series (for Cations) egis Q-Series (for Anions) The sulfonates are sodium salts that act as an anionic counterion for the separation and resolution of positively charged analytes. The sulfonates are available as ion pair concentrates: premixed 0. M solutions of alkyl sulfonates. When diluted to L with HPLC-grade water, a 0mL bottle forms a 0.00 M solution. Five 0mL Bottles 00mL Bottle Description Cat. No. Cat. No. S Pentanesulfonate S Hexanesulfonate S Heptanesulfonate S8 ctanesulfonate S Dodecanesulfonate Method Development Kit S-Series 0mL of S (,,, 8, and ) 000 The Q-series is comprised of quaternary alkyltriethylamines that can be used for the resolution of negatively charged species. This unique set of cationic reagents was developed to complement the Sulfonate Series (S-series). The Quaternary Alkyltriethylamines are available as ion pair concentrates: premixed 0. M solutions of alkyl amines. When diluted to L with HPLC-grade water, a 0mL bottle forms a 0.00 M buffered solution. Five 0mL Bottles 00mL Bottle Description Cat. No. Cat. No. Q Pentyl (TEA) Q Hexyl (TEA) Q Heptyl (TEA) Q8 ctyl (TEA) Q Dodecyl (TEA) Method Development Kit Description Cat. No. Q-Series 0mL of Q (,,, 8, and ) 000 Bulk Ion Pair eagents For Cations Cat. No. Description Grams 00 -Pentanesulfonate, sodium salt g 0 00g 00 -Hexanesulfonate, sodium salt g 0 00g 00 -Heptanesulfonate, sodium salt g 0 00g 008 -ctanesulfonate, sodium salt g 08 00g 00 -Dodecanesulfonate, sodium salt g 00 g For Anions 800 Tetrabutylammonium Phosphate 0mL 0.M, ph. 800 Tetrabutylammonium Phosphate 00mL 0.M, ph. Tech Tip: Guidelines to developing a successful method using ion pair reagents: Select a column endcapped DS (octadecylsilyl) is most common. Use only HPLC-grade water and chromatography grade reagents in mobile phase preparation. Choose the mobile phase components and concentrations that give the best separation. If nonionic components are present in the sample, optimize the resolution prior to attempting ionic separations. Select the appropriate ion pair series to provide the necessary counterion. Use the Q-series for acidic compounds and the S-series for basic compounds. Through a process of elimination, choose the alkyl chain length which results in the best separation. nce the reagent has been selected, adjust the ph of the mobile phase to maximize resolution. Because slight modification of ph can profoundly effect retention and selectivity, make all adjustments in small increments and monitor carefully. Ideally, the ion pair reagent concentration in the mobile phase should be 0.00M. However, small adjustments in reagent concentration may increase retention slightly and optimize the separation. For a complete list of egis reagents, please contact Chrom Tech in MN sales@chromtech.com
2 Agilent Zorbax New! Zorbax apid esolution (HT) High Throughput Columns Up to 9% decrease in analysis time Great resolution Extended ph range, ph to 9 A better way to faster separations! All Agilent Zorbax columns are uniquely optimized for specific applications. ur newest Zorbax columns, apid esolution HT, are packed with totally porous.8µm particles. These columns are optimized for ultra-fast HPLC separations with more resolving power for small molecules. The typical particle size of traditional HPLC columns is µm, but in the past,.0 to.µm particles offered better efficiency with short column lengths. Now, our new apid esolution HT columns with.8µm particles nearly double laboratory productivity compared with columns containing.µm packings. You get even higher resolution and efficiency in very short columns ( to 0mm) ideal for sample screening, LC/MS and many routine analyses. apid esolution HT Columns,.8µm Dimensions StableBond Eclipse (mm) Format SB-C8 XDB-C8. x 0 column x 0 cartridge x 0 cartridge x cartridge x 0 column x 0 cartridge x 0 cartridge x cartridge apid esolution HT Cartridge Hardware Kit Kit includes: () end-fitting assemblies; () 0mm holder; () 0mm holder; () mm holder A B C D Column Conditions Zorbax SB-C8: Mobile Phase: 0% 0mM Na HP, ph.8: 0% ACN Temperature: Ambient Detection: 0nm. Estradiol. Ethynylestradiol. Dienestrol. Norethindrone Flow Analysis Column ate Time Time Dimensions (ml/min) s (,) (min) Savings A.. x 0mm, µm B.. x 00mm,.µm.0.. % C.. x 0mm,.8µm.0.. 8% D.. x 0mm,.8µm.0.. 9% esolution between peaks and Zorbax Method Development (ph -) Low ph - Zorbax StableBond Start method development at low ph, where silanols on a P-HPLC column are protonated. This minimizes peak tailing by eliminating silanol/base interactions. At low ph, basic compounds are positively charged and their retention may be reduced. Acidic compounds may be protonated and have increased retention. etention times are usually stable with small changes in ph, producing a robust method. Volatile mobile phase additives, such as formic acid or trifluoroacetic acid (TFA), are often used at low ph with LC/MS. Mid ph -8 Zorbax Eclipse XDB or Bonus-P Develop methods at ph s at least ph unit above or below the pka to minimiz changes in retention with small changes in ph. Some silica surface H groups become - above ph to ; tailing interactions may be possible. Minimize interaction by selecting an end-capped column, using additives such as TEA (less desirable) or using polar-linked bonded phases. lica breakdown is prevented by innovative bonding chemistry, heavy endcapping and use of x-sil. High ph 8- Extend C8 In this region, basic compounds may be in their free base form. Increased retention and resolution of basic compounds are likely. etention changes little in this region-thus robust methods can be developed. lica breakdown is prevented by innovative bidentate column chemistry, heavy endcapping, use for x-sil and optimum mobile phase. Ammonium hydroxide is an excellent volatile mobile phase modifier at high ph in MN sales@chromtech.com
3 Agilent Zorbax StableBond 80Å Long column lifetime and best reproducibility for low ph separations down to ph! Stable column chemistry allows use at high temperature and low ph without degradation Excellent first choice for method development StableBond packing materials are not endcapped in order to provide exceptional stability and to maximize lifetime and reproducibility under acidic mobile phase conditions. Zorbax StableBond columns are compatible with all common mobile phases including very high aqueous mobile phases. Polar compounds can be analyzed with excellent reproducibility and peak shape because SB-Aq columns have a hydrophilic surface that inhibits phase collapse, even with 00% aqueous mobile phases. Zorbax StableBond Column Specifications Phase ze Area Limits ange Endcapped Load SB-C8 80Å 80m /g 90 C No 0% SB-C8 80Å 80m /g 80 C No.% SB-C 80Å 80m /g 80 C No.0% SB-CN 80Å 80m /g 80 C No.0% SB-Phenyl 80Å 80m /g 80 C No.% SB-Aq 80Å 80m /g 80 C No Proprietary StableBond columns are designed for optimal use at low ph. At ph -8, highest column stability for all silica-based columns is obtained by operating at temperatures <0 C and using low buffer concentrations in the range of M. At mid-range ph, Eclipse XDB and Bonus-P are recommended. 80Å Zorbax StableBond Cartridge Columns Dimensions Particle (USP L) (USP L) (mm) ze (µm) SB-C8 SB-C8. x x x x x x.0, 0/pk x x Cartridge holder Hardware kit requires cartridge holder 0-8 requires hardware kit Å Zorbax StableBond Columns Dimensions Particle (USP L) (USP L) (USP L0) (USP L) (mm) ze (µm) SB-C8 SB-C8 SB-CN SB-C SB-Phenyl SB-Aq. x x x x x x x x x x x x x x x x x x x x x x., /pk x., /pk Guard Hardware Kit (fits both. x. and. x. cartridges) Hardware kit in MN sales@chromtech.com 9
4 Agilent Zorbax StableBond 00Å Designed for the acidic ph mobile phases used in peptide and protein separations Improve hydrophobic peptide separations Applications: Peptides, biopharmaceuticals, hydrophobic peptides, proteins at low ph Zorbax 00StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two key reasons. First, wide-pore, 00Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the, 00SB-C8, SB-C, SB-C8, SB-CN, Nase. Insulin. Cytochrome C. Lysozyme. Parvalbumin, The 00SB-C8, C8, C and CN bonded phases all provide a different separation of this group of polypeptides. This adds an important parameter for quickly optimizing protein separations. The 00SB-CN column offers unique selectivity for more hydrophilic polypeptides. Column Conditions Zorbax 00SB Mobile phase: Linear gradient, -0% B in 0 min. Flow rate:.0ml/min Temperature: 0 C µg each protein. CD. Myoglobin 8. Carbonic Anhydrase 9. S-00β 0. S-00α bonded phase. Second, 00StableBond columns are unmatched in their durability at low ph, such as with the TFA containing mobile phases typically used. 00StableBond columns can also be sued with formic acid and acetic acid mobile phase modifiers. Zorbax StableBond Column Specifications Phase ze Area Limits ange Endcapped Load SB-C8 00Å m /g 90 C No.8% SB-C8 00Å m /g 80 C No.% SB-C 00Å m /g 80 C No.% SB-CN 00Å m /g 80 C No.% StableBond columns are designed for optimal use at low ph. At ph -8, highest column stability for all silica-based columns is obtained by operating at temperatures <0 C and using low buffer concentrations in the range of M. At mid-range ph, Eclipse XDB and Bonus-P are recommended. 00Å Zorbax StableBond Columns Dimensions Particle (USP L) (USP L) (USP L0) (mm) ze (µm) 00SB-C8 00SB-C8 00SB-CN SB-C. x x x x x x x x x x x x x x x, /pk x., /pk x., /pk Guard Hardware Kit (fits both. x. and. x. cartridges) Hardware kit Zorbax Poroshell 00SB Columns for Bioanalytical Chemistry Porous Shell SLID CE µm The revolutionary new Poroshell µm particle combines a solid core with a 00Å porous surface layer bonded with SB-C8. The result is a highly efficient particle for rapid, high-resolution separations of proteins and other large molecules. Large and/or heterogeneous proteins have more complex interactions with the surface of the column packing and show improved peak shapes and resolution on superficially porous particles bonded with shorter phases such as C and C8. The simplified interaction of protein with the surface of the packing is the reason for improved chromatography. For very large, heterogeneous proteins, Zorbax Poroshell C or C8 columns give the best peak shape in a separation 00Å Zorbax StableBond Columns Dimensions Particle Poroshell Poroshell Poroshell (mm) ze (µm) 00SB-C8 00SB-C8 00SB-C. x x x., /pk Guard Hardware Kit (fits both. x. and. x. cartridges) Hardware kit in MN sales@chromtech.com
5 Agilent Zorbax Eclipse XDB Long column life at mid ph with extra dense bonding (XDB) and double endcapping Complementary selectivity to StableBond, Bonus-P or Extend columns Uses Zorbax x-sil silica with uniform wall thickness for better column life at higher ph ph range: recommended to 8, maximum to 9 Zorbax Eclipse XDB Column Specifications Phase ze Area Limits ange Endcapped Load XDB-C8 80Å 80m /g 0 C Double 0% XDB-C8 80Å 80m /g 0 C Double.% XDB-Phenyl 80Å 80m /g 0 C Double.% Zorbax Eclipse XDB Columns Dimensions Particle (USP L) (USP L) (USP L) (mm) ze (µm) XDB-C8 XDB-C8 XDB-Phenyl. x x x x x x x x x x x x x x x x x x x x, /pk Good Peak Shape for Acids, Bases, and Neutrals on Zorbax Eclipse XDB at Mid-pH. Uracil. Butyl Paraben. Propranolol. Naphthalene. Dipropyl Phthalate. Acenaphthene. Amitriptyline 0 0 In the mid-ph region, residual silanols can interact more strongly with analytes, resulting in asymmetric peaks. The Eclipse XDB column provides good peak shape in this difficult ph range. Column Conditions Zorbax Eclipse XDB-C8 Mobile phase: % 0mM NaH P, ph.0, % MeH Flow rate:.0ml/min Temperature: C Detection: UV nm Column Stability Testing. x., /pk x., /pk Eclipse XDB-C8 (Sol-Type) After 8 column volumes Guard Hardware Kit (fits both. x. and. x. cartridges) Hardware kit Zorbax Eclipse XDB Cartridge Columns Dimensions Particle (USP L) (USP L) (mm) ze (µm) XDB-C8 XDB-C8. x x x x x x.0, 0/pk x x x Cartridge holder Hardware kit requires cartridge holder 0-8 requires hardware kit , Column:. x 0mm, µm Mobile Phase: 0% ACN, 0% 0mM phosphate buffer, ph.0 Flow rate:.ml/min Temperature: 0 C Water Symmetry C8 (l-type) After 8 column volumes. Uracil. Nortriptyline. Doxepin. Amitriptyline. Trimipramine Double endcapping, dense bonding, and the durable x-sil particles (sol-type) combine to provide long lifetime at ph.0 when compared to single endcapped sil-gel columns, such as the Waters Symmetry C8 column used here. The conditions used for this test high temperature (0 C) and high salt concentration (0mM), accelerate the dissolution of silica, causing premature failure of the sil-gel type column in MN sales@chromtech.com
6 Agilent Zorbax Bonus-P Excellent peak shape for challenging basic compounds at low and mid ph Unique reversed-phase selectivity Novel bonding technology with embedded polar group and steric protection Usable in 00% aqueous mobile phases The Agilent Zorbax Bonus-P column has a polar amide group embedded in a long alkyl chain. This novel bonding reduces interactions between basic compounds and the silica support, improving peak shape for the most difficult basic compounds. Peak shape and column lifetime are further improbed by triple endcapping. In addition, diisopropyl side groups provide steric protection against acid hydrolysis for good lifetime at low ph. The Bonus-P column provides an alternate selectivity to C8 and C8 alkyl bonded phases. Zorbax Bonus-P Column Specifications Phase ze Area Limits ange Endcapped Load Bonus-P 80Å 80m /g 0 C Triple 9.% Zorbax Bonus-P Columns Particle Bonus-P ze (µm) Cat. No.. x 0mm x 0mm x 0mm x 00mm x mm x 0mm x 0mm x 00mm x 0mm x 0mm x 0mm x 0mm x mm, /pk 8-9 Guard Cartridges (requires hardware kit ). x.mm, /pk x.mm, /pk 8-98 Guard Hardware Kit (fits. and.mm ID cartridges) Hardware kit Bonus-P Can Provide Alternative Selectivity to Alkyl Phases. Prometryne. Tebuthion. Atrazine. Propazine Bonus-P Alkyl-C8 Column Conditions Zorbax Bonus-P Column:. x 0mm Instrument: Agilent 00 Mobile phase: MeH: 0.% TFA (0:0) Flow rate: ml/min. Temperature: Ambient Inj.: µl UV: nm For low ph work, a TFA mobile phase is often preferred over phosphate and is compatible with LC/MS. Dacthal. Diuron. Propanil Bonus-P Provides Excellent Peak Shape for Anorectics at Mid-ange. Phentermine pka 0.. Fenfluramine pka 9.. Impurity Column Conditions Zorbax Bonus-P Columns:. x 0mm Mobile phase: mm K HP, ph./meh:acn (0:0), / Flow rate: ml/min. Inj.: µl UV: nm Anorectics ( Fen-phen ) guard hardware kit shown with guard cartridge and column in MN sales@chromtech.com
7 Agilent Zorbax Extend-C8 High efficiency and long life at high ph up to ph. Unique bidentate bonding and double endcapping provides high ph stability More efficiency and better peak shape than polymerbased columns Improve retention, resolution and peak shape of basic compounds High sensitivity for LC/MS separations of peptides.0e.0e.0e.0e.0e LC/MS Analysis of Angiotensin 80Å Extend-C8 AII + AIII AI Angiotensin I Max: min m/z A. Acidic Conditions: A: 0.% TFA in water B: 0.08% TFA in 80% acetonitrile (ACN).0E C8 lica Support C8 New bidentate C8-C8 bonding for Extend-C8 bonded phase The Agilent Zorbax Extend-C8 column uses a new bidentate C8- C8 column bonding technology to make high ph separations a good choice with a silica-based column. At high ph, non-charged basic compounds will not interact with the silica, so it is possible to do separations with high efficiency, superior peak shape and improved resolution. Some of the mobile phase buffer options for high ph include triethylamine, pyrrolidine, glycine, borate and ammonium hydroxide. Ammonium hydroxide at ph 0. is an excellent mobile phase modifier for the LC/MS of peptides and small molecules with improved sensitivity compared with TFA containing mobile phase at low ph. Zorbax Extend-C8 Column Specifications Phase ze Area Limits ange Endcapped Load Extend-C8 80m /g 0 C.0 -. Double.% Temperature limits are 0 C up to ph 8, 0 C for ph Å or 00Å, as indicated in ordering information table below. Zorbax Extend-C8 Columns Dimensions Particle 80Å 00Å (mm) ze (µm) Extend-C8 Extend-C8. x x x x x x x x x x x x x x x x x, /pk 8-9 Guard Cartridges (requires hardware kit ). x., /pk x., /pk Guard Hardware Kit (fits. and.mm ID cartridges) Hardware kit E 00 Angiotensin I AIII.0E 80 + Max: AII 0.0E 0 + AI.0E min m/z B. Basic Conditions: A: 0mM NHH in water B: 0mM NHH in 80% ACN Both small and large peptides demonstrate selectivity changes at high and low ph. At high ph, due to a change in charge, all three Angiotensins can be resolved. In addition, the spectral clarity of Angiotensin I is dramatically improved at high ph with the ammounium hydroxide mobile phase. The Extend-C8 column can also be used for the analysis of small peptides at high ph. Column Conditions Zorbax 80Å Extend-C8 Column:. x 0mm, µm, Flow rate: 0.mL/min Temperature: C Mobile phase: as indicated Gradient: -0% B in minutes LC/MS: Pos. Ion ESI-Vf 0V, Vcap. kv, N - psi, LPM, C Angiotensin I, II, III;.µL sample (0 pmol each) Angiotensin I: Asp-Arg-Val-Tyr-IIe-His-Pro-Phe-His-Leu Angiotensin I: Asp-Arg-Val-Tyr-IIe-His-Pro-Phe Angiotensin I: Arg-Val-Tyr-IIe-His-Pro-Phe Extend-C8 Provides Good Peak Shape at Low ph These basic compounds are separated on the Extend-C8 at low ph with excellent peak shape. The Extend-C8 column can be used at high and low ph. Column Conditions Zorbax Extend-C8 Column:. x 0mm, µm, 0-90 Mobile phase: 80% mm NaH P, ph.0, 0% MeH Flow rate:.0ml/min Temperature: C Detection: UV nm. Theobromine. Dimethylxanthine. Theophylline. Caffeine Pseudoephedrine and scopolamine are difficult to retain at low and mid ph. Pseudoephedrine is often analyzed by ion exchange methods. The Extend-C8 column retains these compounds in a noncharged ph form at high ph and improves resolution in MN sales@chromtech.com 0 Improved etention, esolution and Peak Shape of Basic Antihistamines on Extend-C8 at High ph Column Conditions Zorbax Extend-C8 Column:. x 0mm, µm, 0-90 Mobile phase: ph : 0% 0mM Na HP, 0% MeH ph : 0% 0mM TEA, 0% MeH Flow rate:.0ml/min Temperature: Ambient Detection: UV nm. Maleate. Chlorpheniramine. Scopolamine. Triprolidine. Pseudoephedrine. Diphenhydramine. Doxylamine, ph
8 Agilent Zorbax Columns Zorbax Gel Filtration Columns Zorbax Ion Exchange Columns apid separations and extended column life time often twice that of other size exclusion columns High recovery for most proteins (>90%) Usable with organic modifiers and denaturants ph range of The silica surface of the Zorbax Gel Filtration columns is stabilized with zirconia, then coated with a monolayer diol phase providing a broad ph range of -8 a highly reproducible, hydrophilic column. Zorbax Gel Filtration Column Specifications Bonded Pore Particle MW Surface Phase ze ze ange Area GF-0 0Å µm,000-00,000 0m /g GF-0 00Å µm 0, ,000 0m /g Additional Specifications for Both Gel Filtration Columns Bonded Phase ph ange Flow ate Max Pressure GF-0 and GF <.0mL/min,000 psi Zorbax ion exchange columns (0Å SAX and 00Å SCX) use Zorbax SIL porous silica microspheres as the stationary phase support; ion exchange groups are attached to a diol backbone. These columns provides highly efficient, rapid separations (pressure limit,000 psi). SCX uses aromatic sulfonic acid SAX uses a trifunctional quaternary amine Typical Applications: SCX basic water soluble compounds SAX aromatic carboxylic acids, aliphatic carboxylic acids, and sulfonic acids Zorbax Ion Exchange Columns Bonded Pore Particle Surface ph Bonded Phase ze ze Area ange Functionality SCX 00Å µm 0m /g Sulfonic acid SAX 0Å µm 00m /g Quaternary amine Separation of a Protein Mixture Zorbax GF-0 Column 8 0 Column Conditions Zorbax GF-0 Column: 9. x 0mm Flow rate: ml/min Eluent: 0mM NaCl/0mM KCl/ 0mM Na HP, ph.0 Detection: UV 0 nm. Mouse IgM 900,000 Da. Bovine Thyroglobulin 0,000 Da. Sweet Potato ß-Amylase 00,000 Da. Bovine Serum Albumin,000 Da. Chicken Albumin,000 Da. Bovine NAse,00 Da. Azide Da Cough-cold emedies Zorbax SCX Column Conditions Zorbax SCX Column:. x 0mm Flow rate: ml/min Eluent: 0. M NaH P, ph. Temperature: 0 C Detection: UV 0 nm. Pyrilamine. Theophylline. Glyceryl Guaicolate. Caffeine. Phenylephrine High-Speed ze Exclusion Separation Zorbax GF-0 Column BSA and BSA Dimers (duplicate injections) 0 Column Conditions Zorbax GF-0 Column: 9. x 0mm Flow rate: ml/min Eluent: PBS, ph. Detection: UV 0 nm Temperature: Ambient. BSA dimer. BSA monomer. Azide Zorbax Gel Filtration Columns Dimensions 0Å 00Å (mm) GF-0 GF-0 9. x x x, /pk x., /pk Guard Hardware Kit Hardware kit for 9. ID Hardware kit for. ID Zorbax Ion Exchange Columns Dimensions (mm) SAX (0Å) SCX (00Å) 9. x x x x x x x Guard Cartridges, /pk (requires hardware kit ). x Guard Hardware Kit Hardware kit in MN sales@chromtech.com
9 Agilent Hypersil Hypersil eversed Phase Columns Hypersil lica Normal Phase Columns Hypersil BDS is both base deactivated and endcapped to improve peak shapes of basic solutes by minimizing interaction with unreacted silanols. Hypersil DS, a very popular packing, adds a monolayer of octadecyl silane (C8) to the Hypersil lica support. Columns are endcapped to reduce silanol interaction. For less retention with similar selectivity, Hypersil MS columns provide a monolayer of dimethyloctylsilane (C8 alkyl chain). eversed Phase Cartridge Columns Cat. No. Description 99BD- BDS-C8,.0µm,.0 x mm 99BD- BDS-C8,.0µm,.0 x mm 99BD- BDS-C8,.0µm,.0 x mm 99B- BDS-C8,.0µm,.0 x 00mm 99B-8 BDS-C8,.0µm,.0 x 0mm 99BD- BDS-C8,.0µm,.0 x mm 99BD-8 BDS-C8,.0µm,.0 x 0mm 99B- BDS-C8,.0µm,.0 x mm 99BD-8 BDS-C8,.0µm,. x 0mm 99BD-9 BDS-C8,.0µm,. x 0mm 998- DS,.0µm,.0 x mm 99D- DS,.0µm,.0 x 0mm 99D- DS,.0µm,.0 x 00mm 998- DS,.0µm,.0 x mm DS,.0µm,.0 x 0mm 99D- DS,.0µm,.0 x mm 99D-8 DS,.0µm,.0 x 0mm DS,.0µm,. x 0mm DS,.0µm,. x 0mm 99B8- BDS-C8,.0µm,.0 x mm 99B8-8 BDS-C8,.0µm,.0 x 0mm 99B8- BDS-C8,.0µm,.0 x mm 99B8- BDS-C8,.0µm,.0 x mm Agilent Cartridge Hardware 0-8 Agilent cartridge hardware eversed Phase Stainless Steel Columns 99BD- BDS-C8,.0µm,. x 00mm 99BD-8 BDS-C8,.0µm,. x 0mm 99BD- DS,.0µm,.0 x mm 99D- DS,.0µm,. x 00mm 99D- DS,.0µm,. x 0mm 99D- DS,.0µm,. x 00mm 99D- DS,.0µm,. x 00mm 99D- DS,.0µm,. x 00mm 99D- DS,.0µm,. x 00mm DS,.0µm,. x 0mm 99M- MS,.0µm,. x 00mm 99M- MS,.0µm,. x 00mm 99M- MS,.0µm,. x 00mm Hypersil eversed Phase Guard Cartridge Columns 99KT-0 DS,.0µm,. x 0mm, /pk DS,.0µm,.0 x mm, 0/pk 99KT-0 DS,.0µm,.0 x 0mm, 0/pk 99KT- MS,.0µm,. x 0mm, /pk 99KT- MS,.0µm,.0 x 0mm, /pk 99BD-0 BDS,.0µm,.0 x mm, 0/pk Agilent Guard Cartridge Hardware 9900CH-00 Cartridge hardware for.mm,.mm ID Hypersil silica columns from Agilent combine a strong, high-purity silica and Agilent s superior packing processes. The strength and reproducibility of these silica particles are also available in an amino phase, Hypersil APS, for normal phase chromatography. Normal Phase Guard Cartridge Columns Cat. No. Description 99AP- Hypersil APS,.0µm,. x 00mm 99AP- Hypersil APS,.0µm,. x 00mm 99AP- Hypersil APS,.0µm,. x 00mm 99SI- Hypersil,.0µm,. x 00mm 99SI- Hypersil,.0µm,. x 00mm 99SI- Hypersil,.0µm,. x 00mm 99SI- Hypersil,.0µm,. x 00mm Normal Phase Guard Cartridge Columns 99KT- Hypersil APS,.0µm,. x 0mm, /pk 99KT- Hypersil APS,.0µm,.0 x 0mm, /pk 99KT- Hypersil,.0µm,. x 0mm, /pk 99KT- Hypersil,.0µm,.0 x 0mm, /pk Agilent Guard Cartridge Hardware 9900CH-00 Cartridge hardware for.mm,.mm ID Column Identification Module (CIM) Attached to an LC column, the column identification module (CIM) communicates with the Agilent 00 Series control module and Agilent ChemStation through a built-in read/write device. Column usage data including serial and batch numbers, dimensions, particle size, packing material and number of injections is recorded electronically in the module (pre-recorded before shipment of Agilent s CIM HPLC columns). You can also record method, plate number, installation date, operator name and other data as needed. With constant updating, column information is always current. Automatic column tracking enhances the Agilent 00 Series features for meeting GLP, cgmp and quality standard requirements, and for achieving fast trouble free validation. Cartridge Columns with CIM Cat. No. Description 98BD- BDS-C8 w/cim,.0µm,.0 x 00mm 98BD-8 BDS-C8 w/cim,.0µm,.0 x 0mm 988- DS w/cim,.0µm,.0 x mm DS w/cim,.0µm,.0 x 0mm Stainless Steel Columns with CIM 988- DS w/cim,.0µm,. x 00mm 988- DS w/cim,.0µm,. x 00mm in MN sales@chromtech.com
Choosing Columns and Conditions for the Best Peak Shape
Choosing Columns and Conditions for the Best Peak Shape What is Good Peak Shape and Why is it Important? Good peak shape can be defined as a symmetrical or gaussian peak and poor peak shape can include
More informationLC and LC/MS Column Selection Flow Chart
LC and LC/MS Column Selection Flow Chart To use the column selection diagram below, simply follow the path for your analyte and mobile phase. At the far right, follow your final column selection to the
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS Zorbax. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS Zorbax Catalogue 9 1 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484 Email:
More informationWilliam E. Barber, Ph.D. Applications Chemist October
William E. Barber, Ph.D. Applications Chemist ctober 0 00 Secrets of Good Peak Shape in HPLC Time: :00 p.m. CET Telephone Number: + 44 0 76 088 Chairperson: John Vis The Secrets of Good Peak Shape in HPLC
More informationThe Secrets of Rapid HPLC Method Development. Choosing Columns for Rapid Method Development and Short Analysis Times
The Secrets of Rapid HPLC Method Development Choosing Columns for Rapid Method Development and Short Analysis Times Rapid Analysis Is More Than Run Time It is developing a method to meet a goal and developing
More informationA Look at Column Choices. Ed Kim Application Engineer Agilent Technologies, Inc February 12, 2009
A Look at Column Choices Does one-size-fit-all? Ed Kim Application Engineer Agilent Technologies, Inc February 12, 2009 Seminar utline lica Surface Chemistry & Physical Characteristics ti lica Bonding
More informationNew Product Update. HPLC Columns
New Product Update HPLC Columns ZORBAX MicroBore HPLC Columns ZORBAX Extend-C18 Columns ZORBAX Bonus-RP Columns Inertsil ODS-2 Cartridge Columns ZORBAX Carbohydrate Analysis Columns ZORBAX Eclipse dsdna
More informationToo Polar for Reversed Phase What Do You Do?
Too Polar for Reversed Phase What Do You Do? June 20, 2013 Mark Powell Columns and Consumables Technical Support Page 1 C8 or C18 Doesn t Always Do the Job Typical reversed phase conditions involve water/buffer
More informationProduct Bulletin. ACE LC/MS and Rapid Analysis HPLC Columns. HPLC Columns
Product Bulletin HPLC Columns ACE LC/MS and Rapid Analysis HPLC Columns 0 mm, 30 mm, 35 mm and 50 mm column lengths.0,., 3.0, 4.0 and 4.6 mm column diameters Configured for High Sample Throughput Specially
More informationProntoSIL C18-EPS Reversed-Phase HPLC Columns
ProntoSIL C8-EPS Reversed-Phase HPLC Columns Provides excellent separation of polar compounds Better peak shape for acids and bases Stabilized bonded phase for rugged, robust HPLC methods More retentive
More informationLogical Method Development for Peptides and Proteins Using RP-HPLC and SEC
Logical Method Development for Peptides and Proteins Using RP-HPLC and SEC Slide 4 001284P1.PPT Seminar Outline 1. Gel-filtration separations by SEC 2. Developing methods for protein and peptide separations
More informationShodex TM ODP2 HP series columns
HPLC Columns Shodex TM ODP2 HP series columns Better retention of highly polar substances Technical notebook No. 6 Contents 1. Introduction 1-1. Specifications 1-2. Eluent Compatibility of ODP2 HP Series
More informationWelcome to our E-Seminar: Choosing HPLC Columns for Faster Analysis Smaller and Faster
Welcome to our E-Sear: Choosing HPLC Columns for Faster Analysis Smaller and Faster High Throughput/Fast LC Requires. Short columns 0 mm or shorter Small particle sizes. µm Rapid Resolution or new.8 µm
More informationPackings for HPLC. Packings for HPLC
Summary of packings for HPLC In analytical HPLC, packings with particle sizes of 3 to 10 µm are preferred. For preparative separation tasks, also particles with diameters larger than 10 µm are applied.
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS SIELC Primesep. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS SIELC Primesep Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0)8 90 660 Fax: +44 (0)8 9 484 Email: sales@hichrom.co.uk
More informationzorbax Packed by Phenomenex Material Characteristics High efficiency Guaranteed quality Phenomenex columns packed with Zorbax
Packed by Phenomenex High efficiency Guaranteed quality Phenomenex columns packed with 7 μm materials offer the advantages of lower cost as well as lower operating backpressures, which result in long column
More informationPeptide and protein analysis by capillary HPLC Optimization of chromatographic and instrument parameters. Application. Angelika Gratzfeld-Huesgen
Peptide and protein analysis by capillary HPLC Optimization of chromatographic and instrument parameters Application Angelika Gratzfeld-Huesgen Abstract This application has been verified using an Agilent
More informationACE Ultra Inert Base Deactivated HPLC Columns
ACE Ultra Inert Base Deactivated HPLC Columns Contents Page ACE HPLC Columns...- Independent Comparison of HPLC Columns #... Independent Comparison of HPLC Columns #... ACE 00Å HPLC Columns Specifications...-
More informationION PAIRING REAGENTS AND BUFFERS
ON PRNG REGENTS ND UFFERS ULTRPURE ON PRNG REGENTS ND UFFERS The dvantages of on Pair hromatography on Pair hromatography is a method for improving the separation of charged analytes. n the resolution
More informationAlltech Alltima HP Introduction
Alltech Introduction High-Stability, High-Purity, High-Performance, Low-Bleed Columns for Demanding Applications Better Peak Symmetry high-purity silica eliminates peak tailing problems Long Column Life
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS HALO and HALO-5. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS HALO and HALO- Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG PE, UK Tel: + (0)8 90 0 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationHPLC Method Development with Eclipse Plus: Standard Practices and New Columns. Agilent Technologies
HPLC Method Development with Eclipse Plus: Standard Practices and New Columns Agilent Technologies What are Some Standard Method Development Practices?. Follow preferred method development scheme and do
More informationHypersil BDS Columns TG 01-05
TG 0-0 Hypersil BDS Columns Introduction Hypersil BDS columns have gained a reputation over the years as one of the most robust, reproducible and reliable HPLC column brands available. This Technical Guide
More informationSepax SRT -C, Zenix -C SEC Phases
Sepax SRT -C, Zenix -C SEC Phases Complimentary Phases to SRT for Derivatized Monoclonal Antibodies General Description Stationary Phase Structure SRT, Zenix, SRT-C and Zenix-C SEC phases developed based
More informationDoes one-size-fit-all?
A Look at Column Choices: Does one-size-fit-all? Bill Champion Agilent Technologies, Inc. 800-227-9770 opt 3/opt3/opt 2 william_champion@agilent.com Page 1 Separation Techniques Hydro - philic Volatile
More informationEfficiently analyze your most challenging compounds. Deliver high-quality results faster. Agilent ZORBAX Column Selection Guide for HPLC
Efficiently analyze your most challenging compounds. Deliver high-quality results faster. Agilent ZORBAX Column Selection Guide for HPLC Agilent ZORBAX Column Selection Guide for HPLC Here it is your single
More informationHow proteins separate on reverse-phase HPLC
1 Reverse Phase How proteins separate on reverse-phase HPLC RP chromatography separates proteins through the interaction of the hydrophobic foot of the protein with a nonpolar surface of the particle RP
More informationHPLC Method Development: Standard Practices and New Columns
HPLC Method Development: Standard Practices and New Columns Edward Kim Application Engineer Agilent Technologies, Inc. March 8, 29 What are Some Standard Method Development Practices?. Follow preferred
More informationProntoSIL HPLC Columns
ProntoSIL HPLC Columns For Technical Assistance Call -800--708 analytical inc. To Order and For More Information: Call FAX -800--708-0-8-99 -0-8-99 We ship nearly 00% of your orders the same day they are
More informationInertsil ODS-EP Technical Information
Technical Information Advantages of The selectivity is completely different from those of conventional columns such as ODS column due to its specific polar group in the stationary phase. Durability can
More informationACE. For increased polar retention and alternative selectivity. Alternative selectivity for method development
ACE C8-Amide For increased polar retention and alternative selectivity Alternative selectivity for method development Improved separations with polar, acidic, basic and phenolic compounds High efficiency
More informationBarry E. Boyes, Ph.D. Consumables and Accessories Business Unit May 10, 2000
Barry E. Boyes, Ph.D. Consumables and Accessories Business Unit May 0, 000 HPLC Column Troubleshooting What Every HPLC User Should Know :00 a.m. EST Telephone Number: 86-650-06 Chair Person: Tim Spaeder
More informationImprove Peak Shape and Productivity in HPLC Analysis of Pharmaceutical Compounds with Eclipse Plus C8 Columns Application
Improve Peak Shape and Productivity in HPLC Analysis of Pharmaceutical Compounds with Eclipse Plus C8 Columns Application Pharmaceuticals Authors John W. Henderson Jr., Nona Martone, and Cliff Woodward
More informationBreaking the speed limit: Fast, high-resolution peptide and tryptic digest separations using fused-core particles
Breaking the speed limit: Fast, high-resolution peptide and tryptic digest separations using fused-core particles Stephanie Schuster 1, Barry Boyes 1,2, and Darryl Johnson 2 1 Advanced Materials Technology,
More informationpenta-hilic UHPLC COLUMNS
penta-hilic UHPLC COLUMNS penta-hilic Highly retentive, proprietary penta-hydroxy-ligand Excellent peak shape for polar compounds with a variety of functional groups: acids, bases, zwitterions strong and
More informationLC Column Troubleshooting. Do you have an equivalent column for my LC column?
LC Column Troubleshooting Do you have an equivalent column for my LC column? LC columns from different suppliers may have very different retention properties even if the bonding is the same. Although there
More informationBringing uhplc Performance to the Separation of Peptides
P e p t i d e E S - C 1 8 U H P L C C o l u m n s Bringing uhplc Performance to the Separation of Peptides Compatible with UHPLC and conventional HPLC equipment. Sumpfstr. 3, CH-6300 Zug; Fax: 041 748
More informationSuperficially Porous Silica Particles with Wide Pores for Biomolecular Separations
Superficially Porous Silica Particles with Wide Pores for Biomolecular Separations B. M. Wagner, S. A. Schuster, B. E. Boyes, J. J. Kirkland Advanced Materials Technology, Inc. Wilmington, DE, USA Study
More informationReversed Phase Chromatography: Mobile phase Considerations
Reversed Phase: Mobile Phase Considerations Dr. www.forumsci.co.il/hplc Example of a Reversed Phase Method with UV Detection 1 Chromatographic Process B+A Mobile phase Elution through the Column-movie
More informationAdvantages of polymerbased. an alternative for ODS
Advantages of polymerbased HPLC columns an alternative for ODS Yukiko Higai Shodex, 1 Content (1) Comparison between Silica and Polymer-based RP columns (2) Characteristics of Polymer RP columns - an example:
More informationMethod Development in Solid Phase Extraction using Non-Polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples
Technical Note 101 Method Development in Solid Phase Extraction using Non-Polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples This technical note includes by specific information on the extraction
More informationComparison of different aqueous mobile phase HPLC techniques
June 009 ewsletter Pharmaceutical Analysis: What is Your Problem? SIELC Technologies, Inc., Prospect Heights, IL 0070 Pharmaceutical analysis involves liquid chromatography of various compounds, from active
More informationOptimisil HPLC & UPLC Column catalogue
Optimisil HPLC & UPLC Column catalogue Analytical Hub Plot No: 37, P & T Colony, Vikrampuri, Secunderabad 500009 Tel: 040-64621313, Mobile: +91 7799931313 Website: www.analyticalhub.com email: contact@analyticalhub.com
More informationpenta-hilic UHPLC COLUMNS
penta-hilic UHPLC COLUMNS Highly retentive, proprietary penta-hydroxy-ligand penta-hilic Excellent peak shape for polar compounds with a variety of functional groups: acids, bases, zwitterions strong and
More informationState-of-the-art C18 HPLC Columns
An HPLC GL Sciences Newest and Most Advanced ODS Phase-New For 00 State-of-the-art C HPLC s Improved Peak Shapes and Heights Enhancing Sensitivity High Resolution Fast Equilibration Compatible with 00%
More informationReversed Phase Solvents
Part 1. General Chromatographic Theory Part 2. verview of HPLC Media Part 3. The Role of the Mobile Phase in Selectivity Part 4. Column Care and Use Reversed Phase Solvents 2 Solvents for RP Chromatography
More informationLC Technical Information
LC Technical Information Method Transfer to Accucore.6 μm Columns Containing solid core particles, which are engineered to a diameter of.6μm and a very narrow particle size distribution; Accucore HPLC
More informationHPLC Method Development: Standard Practices and New Columns. Agilent Technologies February 2007
HPLC Method Development: Standard Practices and New Columns Agilent Technologies February 7 What are Some Standard Method Development Practices?. Follow preferred method development scheme and do hands-on
More informationProPac WCX-10 Columns
ProPac WCX-10 Columns Guidance for column use Tips to maximize column lifetime ProPac WCX-10 Column Tips and Tricks This guide provides essential information and invaluable guidelines for mobile phases,
More informationcolumns Acclaim Mixed-Mode WCX-1 for Separating Basic Molecules
columns Acclaim Mixed-Mode WCX- for Separating Basic Molecules The Acclaim Mixed-Mode WCX- is a novel, high-efficiency, silica-based column specially designed for separating various basic analytes. This
More informationHamilton. Reversed-Phase Anion Exchange Cation Exchange Ion Exclusion
HPLC Columns Agilent for Small Molecule InfinityLab Poroshell 0 ZORBAX Eclipse Plus ZORBAX Eclipse XDB ZORBAX 80Å StableBond ZORBAX 80Å Extend-C8 ZORBAX Bonus-RP ZORBAX Eclipse PAH ZORBAX HILIC Plus ZORBAX
More informationInertSustainSwift C8
HPLC, LC/MS Columns InertSustainSwift TM C8 New! InertSustainSwift C8 is an octyl group (C8) bonded column offering the same extreme inertness to any type of compounds just like InertSustainSwift C8, which
More informationMethod Development Kits
Method Development Kits sales representative for ordering information or contact...77 Method Development Kits First column choice for Pharma Development USP L C Method Development Kits Maximize retention
More informationAnalysis - HPLC A.136. Primesep 5 µm columns B.136
Primesep 5 µm columns Primesep columns feature double functionality of the bonding i.e : alkyl chain with anionic or cationic group, chelating group. This feature creates unique selectivities when using
More informationAcclaim Mixed-Mode WCX-1
Acclaim Mixed-Mode WCX-1 Product Manual for the Acclaim Mixed-Mode WCX-1 Column Page 1 of 28 PRODUCT MANUAL for the Acclaim Mixed-Mode WCX-1 Columns 4.6 x 150 mm, P/N (068353) 4.6 x 250 mm, P/N (068352)
More informationA Look at Agilent HPLC Columns Choices
A Look at Agilent HPLC Columns Choices Brno & Prague, May 29-30 2018 Giorgio Ferlat IDO Product Specialist Chemistries and Consumable 1 Considerating Column Properties for Column Selection Silica Particle
More informationfor Acclaim Mixed-Mode HILIC-1 Column
for Acclaim Mixed-Mode HILIC-1 Column Product Manual for ACCLAIM Mixed-Mode HILIC-1 Page 1 of 17 Product Manual for ACCLAIM Mixed-Mode HILIC-1 Column 5µm, 4.6 x 250mm, P/N 066844 5µm, 4.6 x 150mm, P/N
More informationFast Separation of Vastly Different Compounds by Isocratic HPLC
Fast Separation of Vastly Different Compounds by Isocratic HPLC Aimee N. Heyrman and Yury Zelechonok Resolution Systems, Inc. 590 E. 32nd St. Holland. MI, 49423 SIELC Technologies, 15 E. Palatine Rd, Suite
More informationUptisphere CS Evolution Core Shell columns for fast & highly efficient identification & quantification of small molecules
Uptisphere CS Evolution Core Shell columns for fast & highly efficient identification & quantification of small molecules www.interchim.com www.blog.interchim.com www.forum.interchim.com www.facebook.com/interchim
More informationACE SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE SuperC8 TM Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS compatible buffers between ph.. High efficiency µm, µm,
More informationRP C18 column with feature of a silanol group
RP C8 column with feature of a silanol group lanol Activity Controlled C8 Column Sunrise ctacosyl C8) Sunrise ctadecyl C8) Sunrise ctadecyl-sac C8-SAC has an interaction of silanol groups ChromaNik Sunrise
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS Vydac. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS Vydac Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG PE, UK Tel: + (0)8 90 660 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationInertSustainSwift C8
Physical Properties Silica Particle Size Surface Area Pore Size Pore Volume Bonded Phase End-capping Carbon Loading ph Range USP Code :ES (Evolved Surface) Silica Gel :.9 μm, μm, μm :00 m /g :00 Å (0 nm)
More informationThermo Scientific Accucore XL HPLC Columns. Technical Manual
Thermo Scientific Accucore XL HPLC Columns Technical Manual Thermo Scientific Accucore XL HPLC Columns Based on Core Enhanced Technology using µm solid core particles, Accucore XL HPLC columns allow users
More informationP R O D U C T B U L L E T I N. Fused-Core particle technology for hyper-fast and super-rugged HPLC columns
P R O D U C T B U L L E T I N Fused-Core particle technology for hyper-fast and super-rugged HPLC columns HALO column packings are not made the typical way. Instead, the particles packed into HALO columns
More informationACE SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE SuperC8 TM Ultra-Inert UHPLC and HPLC s with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS compatible buffers between ph.. High efficiency µm, µm, µm and
More informationACE Excel SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE Excel SuperC8 TM Ultra-Inert UHPLC and HPLC s with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS-compatible buffers between ph.. High efficiency µm, µm,
More informationCapital HPLC Columns and HPLC Cartridges. Optimal HPLC Range
Capital HPLC Columns and HPLC Cartridges Specialty Columns HPLC Columns & HPLC Cartridges Download Capital Catalog Adsorbosphere Apex Chromegabond Diasogel Exsil Hypersil Inertsil ODS-2 Partisil Rosil
More informationAn HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint
An HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint An important new column in the Inertsil series Bonded-Phase Structure Perfect balance of retention for both polar
More informationA C18 Silica Column With Exceptional Temperature and ph Stability
A C18 lica Column With Exceptional Temperature and ph Stability Brian A. Jones 1, Stephanie J. Marin 1, Jody Clark 1, Nathan L. Porter 1, J. Andreas Lippert 2, and Todd M. Johnson 2 1. Selerity Technologies,
More informationMethod Development with ZirChrom's Ion Exchange Phases
For Peak Performance ZIRCHROM ION EXCHANGERS Phases for Sugars and Proteins Wide Range of Ion Exchange Selectivity No Shrinking or Swelling Use Any Organic Solvent Significantly Higher Efficiency than
More informationHigh Resolution Fast LC
High Resolution Fast LC Easier Than You Think Rita Steed LC Columns Application Engineer May 9, 2013 What is High Resolution Fast LC? Maintain Resolution with Faster Run Time Increased Resolution with
More informationOrosil HPLC Columns. OroSil HPLC columns are designed for the separation of polar, semi-polar, and nonpolar compounds at low to medium ph.
Orosil OroSil HPLC columns are designed for the separation of polar, semi-polar, and nonpolar compounds at low to medium ph. Excellent organic base selectivity with very low asymmetry values Compatible
More informationExceptional Selectivity of Agilent ZORBAX Eclipse Plus Phenyl-Hexyl Columns to Separate Estrogens
Exceptional Selectivity of Agilent ZORBAX Eclipse Plus Phenyl-exyl Columns to Separate Estrogens Application Note Pharmaceutical, Environmental Authors John W. enderson Jr. and William J. Long Agilent
More informationThe Theory of HPLC. Ion Pair Chromatography
The Theory of HPLC Ion Pair Chromatography i Wherever you see this symbol, it is important to access the on-line course as there is interactive material that cannot be fully shown in this reference manual.
More informationfor Acclaim Mixed-Mode WCX-1
for Acclaim Mixed-Mode WCX- Product Manual for the Acclaim Mixed-Mode WCX- Column Page of 8 Product Manual for Acclaim Mixed-Mode WCX- Columns Acclaim Mixed-Mode WCX-, 3µm, Analytical column, 3.0 x 50mm
More informationSunniest C18 Sunniest RP-AQUA Sunniest C8 Patent pending
Patent pending Website : www.chromtech.net.au E-mail : info@chromtech.net.au telno : 0 97 0... in AUSTRALIA A Novel Bonding Technique (patent pending) An unique trifunctional silyl-reagent was developed
More informationPart 2. Overview of HPLC Media
Part 1. General Chromatographic Theory Part 2. Overview of HPLC Media Part 3. The Role of the Mobile Phase in Selectivity Part 4. Column Care and Use 1 HPLC Particle Technology Core-Shell Particle Fully
More informationRonald E. Majors, Ph.D. Product Marketing Specialist Consumables and Accessories Business Unit May 31, 2000
Ronald E. Majors, Ph.D. Product Marketing Specialist Consumables and Accessories Business Unit May 31, 2000 Logical Method Development for Small Molecular Weight Ionizable Compounds using RP-HPLC 11:00
More informationHigh Performance Liquid Chromatography
Updated: 3 November 2014 Print version High Performance Liquid Chromatography David Reckhow CEE 772 #18 1 HPLC System David Reckhow CEE 772 #18 2 Instrument Basics PUMP INJECTION POINT DETECTOR COLUMN
More informationHigh Performance Liquid Chromatography
Updated: 3 November 2014 Print version High Performance Liquid Chromatography David Reckhow CEE 772 #18 1 HPLC System David Reckhow CEE 772 #18 2 1 Instrument Basics PUMP INJECTION POINT DETECTOR COLUMN
More informationAgilent s New Weak Anion Exchange (WAX) Solid Phase Extraction Cartridges: SampliQ WAX
Agilent s New Weak Anion Exchange (WAX) Solid Phase Extraction Cartridges: SampliQ WAX Technical Note Agilent s SampliQ WAX provides Applications for strongly acidic, acidic and neutral compounds Excellent
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS SeQuant ZIC-HILIC. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS SeQuant ZIC-HILIC Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 PE, UK Tel: + (0)8 90 660 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationAnalysis of Metals, Halides, and Inorganic Ions Using Hydrophilic Interaction Chromatography
Application Note Inorganic Ions, Water Testing, Minerals, Metals, Basic Chemicals Analysis of Metals, Halides, and Inorganic Ions Using Hydrophilic Interaction Chromatography Authors Anne Mack, Adam Bivens
More information8. Methods in Developing Mobile Phase Condition for C18 Column
I. HPLC Columns Technical Information 8. Methods in Developing Mobile Phase Condition for C18 Column Introduction In reversed phase HPLC, octadecyl group bonded silica columns (C18, ODS) are the most widely
More informationNew 5-micron HALO-5 columns based on Fused- Core particle technology boost the performance of HPLC. Compared to other HPLC columns, HALO-5 columns
New 5-micron HALO-5 columns based on Fused- Core particle technology boost the performance of HPLC. Compared to other HPLC columns, HALO-5 columns have: the highest plate number versus any other 5-micron
More informationOverview Applications of NUCLEODUR HPLC Phases
verview Applications of HPLC Phases Phase Specification Characteristics* Stability Structure A B C octadecyl phase, high density coating multi-endcapping 8% C USP L ph stability -, suitable for LC/MS (Si-
More informationGemini-NX Performance paired with Axia Technology
Si Si Si Si Si Si Si C Si Si Si Si Si Gemini-NX Performance paired with Axia Technology Gemini -NX Axia Packed Technology Improves Yield, Throughput, and Column Lifetime Ideal Purification Platform While
More informationSRT -10/10C (10 µm ) Fast Protein Purification For FPLC, HPLC and Prep LC. Sepax Technologies, Inc. Preparative Size Exclusion Chromatography
Sepax Technologies, Inc. Preparative Size Exclusion Chromatography SRT -0/0C (0 µm ) Fast Protein Purification For FPLC, HPLC and Prep LC Better Surface Chemistry for Better Separation SRT -0/0C Size Exclusion
More informationTips and tricks to increase HPLC column lifetime. Dr. Frank Michel
Tips and tricks to increase HPLC column lifetime Dr. Frank Michel Overview How to influence HPLC column life time? HPLC method Helpful accessories Best practice 2 HPLC Method Mobile Phase Mobile phase
More informationKromasil. for your analytical HPLC. Kromasil. The way to peak performance in liquid chromatography
Kromasil for your analytical HPLC Kromasil The way to peak performance in liquid chromatography Kromasil is known, worldwide, for its high performance and excellent total economy in preparative and industrial
More informationPhenyl-Hexyl. UHPLC Columns. Alternate, complementary selectivity to C18 and C8 bonded phases
Phenyl-Hexyl UHPLC Columns Alternate, complementary selectivity to C8 and C8 bonded phases Particularly recommended for compounds containing aromatic groups Excellent bonded phase stability for durable,
More informationH P L C C O L U M N S
H P L C C O L U M N S New, HxSil TM C8 and C8 HPLC Columns A Word about HPLC Columns For over 0 years Hamilton HPLC columns have been solving problems no other columns could. Now we have introduced HxSil
More informationInertsil Technical Library
HPLC COLUMN INERTSIL TECHNICAL LIBRARY H P L C C o l u m n Inertsil Technical Library Inertsil ODS-4, C8-4 Comparison of Performance List of Compared Columns Experimental Explanation, Analytical Conditions
More informationPacked Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS. Table of Contents
No. 089 SEPARATION REPORT Packed Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS Table of Contents 1. Introduction 1 2. Column Specification 1 3. Features of Packing Materials
More informationHPLC UHPLC Columns. Dikma Technologies Inc.
Technologies Inc. About HPLC UHPLC Columns has over years of R&D experience in HPLC / UHPLC columns. HPLC / UHPLC columns have been widely used by pharmaceutical manufacturers. We offer columns of ultimate
More informationNOTES. Zenix SEC-80. Sepax Zenix SEC-80 provides a valuable solution
Having trouble applying size exclusion chromatography to peptides under 1, Da? What if there was a size exclusion column specifically designed for small protein and peptide separations? Sepax Zenix SEC-8
More information( )( ) Selectivity Choices in Reversed-Phase Fast LC. Introduction. R s = 1 a 1 k 4 a 1 + k
Selectivity Choices in Reversed-Phase Fast LC Luisa Pereira, Monica Dolci, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20543 Key Words Accucore, Hypersil GOLD, Syncronis, Column Chemistry,
More informationStrength in Technology
Strength in Technology Fortis C8 Excellent Peak Shapes Superb Quantitation ph Range - Fully Scalable 3,5,0μ C8 7% Fortis C8 is designed to provide characteristics which will aid in increased productivity
More informationLC Columns with Liquid Separation Cell Technology
Obelisc LC Columns with Liquid Separation Cell Technology Obelisc HPLC Columns - Liquid Separation Cell Technology Introduction Obelisc HPLC columns are the latest, innovative columns from SIELC Technologies,
More information