Contents. Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 1
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1 Contents 1. Introduction 2. Fluids 3. Physics of Microfluidic Systems 4. Microfabrication Technologies 5. Flow Control 6. Micropumps 7. Sensors 8. Ink-Jet Technology 9. Liquid Handling 10.Microarrays 11.Microreactors 12.Analytical Chips 13.Particle-Laden Fluids a. Measurement Techniques b. Fundamentals of Biotechnology c. High-Throughput Screening Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 1
2 3. Physics of Microfluidic Systems 1. Navier-Stokes Equations 2. Laminar and Turbulent Flow 3. Fluid Dynamics 4. Fluid Networks 5. Energy Transport 6. Interfacial Surface Tension 7. Electrokinetics Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 2
3 3.2. Laminar and Turbulent Flow 1. Critical Reynolds Number 2. Shear-Driven Laminar Flow 3. Couette Flow 4. Laminar PDF through a Tube 5. Laminar PDF through a Gap 6. Irrotational Flow 7. Centrifugal-Force Driven Flow 8. Effects in Laminar Flows 9. Turbulent Flows Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 3
4 Three types of flow conditions Laminar Low flow velocities Smooth sliding of adjacent layers Field of velocity vectors constant in time Turbulent Curling of field lines Mixing between adjacent layers Unpredictable" development of field of velocity vectors Flow patterns increasingly turbulent towards high velocities Sometimes laminar flow preserved up to higher velocities Periodic flow 3rd flow regime Surface waves Acoustic waves Critical Reynolds Number All three flow types solutions of NS-equation Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 4
5 Perturbation Analysis Transition from laminar to turbulent flow regime Mathematical perturbation analysis Prediction whether velocity distribution belongs to distinct flow regime Ansatz Known solution of NS-equation (guessed or measured) Superimposing small perturbation Product of - Amplitude - Oscillatory factor - Exponential term Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 5
6 Perturbation Analysis Properties Locally varying amplitude A and constant for given problem Insertion of perturbed solution in NS as initial velocity field Result: First order equations of and Sign of indicates decay of perturbation into v 0 Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 6
7 Critical Reynolds Number Condition = 0 defines critical Reynolds number Re* Re < Re * Perturbations damped in time Re > Re* Exponential growth of perturbations in time Perturbation theory not valid Unpredictable behavior of velocity field Transition point Re = Re* Flow oscillates between two flow regimes As Re increases further, turbulent character of flow increases Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 7
8 Critical Reynolds Number Re* ranges between 1 and 100,000 Re* depends on Material properties (density, viscosity) Boundary conditions Critical velocity Microdevice l = 100 µm v* = 25 m s -1 Hardly reached in microdevices Re* geometries Sphere: 2320 Flow parallel to plate: Re * = 500,000 Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 8
9 3.2. Laminar Flow Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 9
10 Critical Reynolds Number Transition point also depends on Initial velocity field Experimental environment Domain Re < Re* No survival of initial turbulences Domain Re > Re* Laminar flow still possible under certain conditions Turbulences hampered by - Smooth walls - Smooth endings at orifices Laminar conditions up to Re = 100,000 Re > 100,000 - Thermal motion of molecules sufficient to trigger transition to turbulence Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 10
11 Shift of Re* in MF Systems In MF-systems Channel diameter 100 µm Flow velocity v = 10 mm s -1 Flow rate Av = 6 µl min -1 Re ~ 1 << Re * ~ 2300 Always laminar flow in MF-systems? Departure of Reynolds theory in microworld Much higher surface-to-volume ratios Higher sensitivity to surface roughness Reliable results only with smooth surfaces and stabilized pumping Some results indicate early departure from laminar flow regime < Re * < 900 or 200 < Re * < 700 Avoiding artificially induced nucleation of turbulence - Smooth walls - Steady pumping Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 11
12 3.2. Laminar and Turbulent Flow 1. Critical Reynolds Number 2. Shear-Driven Laminar Flow 3. Couette Flow 4. Laminar PDF through Tube 5. Laminar PDF through Gap 6. Irrotational Flow 7. Centrifugal-Force Driven Flow 8. Effects in Laminar Flows 9. Turbulent Flows Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 12
13 Shear-Driven Laminar Flow Flow imposed on fluid by mere shear forces Viscous fluid sandwiched between two plates Wall at x = 0 at rest Wall at x = d moving at speed v 0 = const. in z-direction Viscous force density Stationary flow Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 13
14 Shear-Driven Laminar Flow Boundary conditions Linear flow profile Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 14
15 Shear-Driven Laminar Flow Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 15
16 Prandtl Boundary Layer d increases until Re > Re* Turbulent regime Laminar regime restricted to Tiny layer < d near moving body "Prandtl layer" Diffusion-limited mass and heat transfer Decisive impact on mass and heat exchange in macrosystems Estimate for thickness by energy analysis Viscous work Spent when body traveling at v 0 covers distance of its own length l Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 16
17 Prandtl Boundary Layer Setting adjacent fluid into motion requires kinetic energy Assuming linear flow profile within Prandtl layer Setting equal kinetic energy and viscous work yields Typical MF-values: l = 1cm, d = 100 µm and Re = 1 1 cm >> d Fully developed Prandtl layer therefore not found in MF systems Attention - Re increases with speed of flow Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 17
18 3.2. Laminar and Turbulent Flow 1. Critical Reynolds Number 2. Shear-Driven Laminar Flow 3. Couette Flow 4. Laminar PDF through Tube 5. Laminar PDF through Gap 6. Irrotational Flow 7. Centrifugal-Force Driven Flow 8. Effects in Laminar Flows 9. Turbulent Flows Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 18
19 Couette Flow Azimuthal symmetry Purely azimuthal fluid motion Cylindrical coordinates (r,, z) Velocity field v(r) Pressure distribution p Symmetry reduces NS-equations and continuity equation to Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 19
20 Azimuthal symmetry Purely azimuthal fluid motion Cylindrical coordinates (r,, z) Velocity field v(r) Ansatz Couette Flow Solution small ~ r Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 20
21 3.2. Laminar and Turbulent Flow 1. Critical Reynolds Number 2. Shear-Driven Laminar Flow 3. Couette Flow 4. Laminar PDF through Tube 5. Laminar PDF through Gap 6. Irrotational Flow 7. Centrifugal-Force Driven Flow 8. Effects in Laminar Flows 9. Turbulent Flows Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 21
22 Laminar PDF through Tube Pressure-driven flow Important phenomenon in nature E.g., transport of nutrients in plants and animals by heart Law of Hagen-Poisseuille Pressure drop Throughput Symmetry Parabolic flow profile Cylindrical symmetry Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 22
23 Laminar PDF through Tube Pressure forces Viscous forces Relationship for stationary flow (dv z /dt = 0) F p = F Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 23
24 Laminar PDF through Tube Integration Extension of auxiliary cylinder of radius r to tube radius r 0 Flow velocity profile Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 24
25 Laminar PDF through Tube Maximum velocity (in center at r = 0) Average velocity Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 25
26 Flow Rate Volumetric flow I V determined by integration of v z (r) da over r 0 Law of Hagen-Poiseuille I V scales with r 4 Average velocity Alternative expression for Reynolds number Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 26
27 Throughput I V ~ r 4 = A 2 Hagen-Poiseuille A 0 /4 A 0 I 0,V ~ A 0 2 I V ~ 4 (A 0 / 4) 2 = ¼ I 0V I V ~ N (A 0 / N) 2 = (1/N) I 0V Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 27
28 Hydraulic Diameter Based on law of Hagen-Poiseuille for cylindrical geometry PDF through duct with non-circular cross-section Equivalent hydraulic diameter Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 28
29 Hydraulic Diameter Round tube Square tube Edge length Annular geometry Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 29
30 3.2. Laminar and Turbulent Flow 1. Critical Reynolds Number 2. Shear-Driven Laminar Flow 3. Couette Flow 4. Laminar PDF through Tube 5. Laminar PDF through Gap 6. Irrotational Flow 7. Centrifugal-Force Driven Flow 8. Effects in Laminar Flows 9. Turbulent Flows Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 30
31 Laminar PDF through Gap Pressure-driven flow No (external) shear or volume forces Parallel plates Laminar regime Pressure gradient antiparallel to direction of flow No-slip conditions Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 31
32 Laminar PDF through Gap Rectangular element Width 2x Length l Depth b Cross section A x = b l Fore-part A z = 2 x b Total velocity gradient across element 2 dv / dx +/-x Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 32
33 Differential relationship Laminar PDF through Gap Parabolic flow profile Peak velocity Overall volume flow rate I V per channel width y Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 33
34 3.2. Laminar and Turbulent Flow 1. Critical Reynolds Number 2. Shear-Driven Laminar Flow 3. Couette Flow 4. Laminar PDF through Tube 5. Laminar PDF through Gap 6. Irrotational Flow 7. Centrifugal-Force Driven Flow 8. Effects in Laminar Flows 9. Turbulent Flows Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 34
35 Irrotational Flows Vorticity Vanishes in irrotational flows Vector identity Vanishing divergence of vorticity For vanishing vorticity, i.e. irrotational flow, v can be written Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 35
36 Potential Flow Theory Basic building blocks Set of special flow schemes Analogous to multipole concept in electrodynamics Mathematical point of view Special instances of Green s function Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 36
37 Velocity Potentials (2-dim.) Simplification 2-dim. velocity field v = (v x, v y ) Velocity potential Scalar Stream function Scalar monopole dipole Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 37
38 Hele-Shaw Table Visualization of basic 2-dim. flows Uniform stream over floor to drain Bottles Raised or lowered to adjust gravitational pressure Connected to through holes 2-dim. flow (top view) Sources and drains (monopoles) Holes Doublets (dipoles) Source and sink very close to each other Bottles spaced by same distance above and below floor Sometimes transparent cover to ensure uniform depth Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 38
39 Continuity equation Irrotational flow Navier-Stokes Rewritten Bernoulli Equation Using vector analysis General form of Bernoulli Integration in space vanishing vorticity Bernoulli Stationary conditions Integration in space Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 39
40 3.2. Laminar and Turbulent Flow 1. Critical Reynolds Number 2. Shear-Driven Laminar Flow 3. Couette Flow 4. Laminar PDF through Tube 5. Laminar PDF through Gap 6. Irrotational Flow 7. Centrifugal-Force Driven Flow 8. Effects in Laminar Flows 9. Turbulent Flows Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 40
41 Centrifugal-Force Driven Flow Flow Profile Laminar flow only driven by centrifugal forces Fluidic duct with radius r 0, angular frequency Stationary conditions, incompressible fluids No-slip boundary conditions Neglecting inertia and pressure effects Solution: z-dependent flow profile At center r z Velocity profile typically more flat than in PDF Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 41
42 Comparison to PDF CD PDF Parabolic velocity profiles Relation between steepness of velocity profiles Example - z = l = 1 cm - = 1000 kg m -3 (water) p = 1000 hpa - = 500 rpm (single speed CD player) v^ PDF profile much steeper Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 42
43 3.2. Laminar and Turbulent Flow 1. Critical Reynolds Number 2. Shear-Driven Laminar Flow 3. Couette Flow 4. Laminar PDF through Tube 5. Laminar PDF through Gap 6. Irrotational Flow 7. Centrifugal-Force Driven Flow 8. Effects in Laminar Flows 9. Turbulent Flows Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 43
44 Taylor Dispersion Axial dispersion of solute in laminar flow Dispersion of drugs in blood flow Situation Steady state flow Round tube Hypothetical absence of diffusion Solute follows flow profile Molecular diffusion Counteracts dispersion Axial spreading at D eff t Radial diffusion exchanges solute molecules between layers MF example v = 1 mm s -1, r 0 = 100 µm, D = 3 x 10-9 m 2 s -1 Second term prevails over unity Effective constant for axial diffusion ~ D(1 + c D 2 ) Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 44
45 Hydrodynamic Focusing Situation Microscopic tip at end of capillary Sucking in liquid from larger vessel Laminar regime Full solid angle projected onto tiny orifice cross section Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 45
46 Example: Hydrodynamic Focusing Ink dispenser near orifice of capillary Vertical position within capillary adjusts to transversal shift of dispenser Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 46
47 Reversed Experiment Fluid plug expelled from orifice of capillary into larger tank Small velocity Laminar High velocity Turbulent Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 47
48 Application to Cytometry and Mixing Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 48
49 3.2. Laminar and Turbulent Flow 1. Critical Reynolds Number 2. Shear-Driven Laminar Flow 3. Couette Flow 4. Laminar PDF through Tube 5. Laminar PDF through Gap 6. Irrotational Flow 7. Centrifugal-Force Driven Flow 8. Effects in Laminar Flows 9. Turbulent Flows Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 49
50 Turbulent Flows Turbulent flow in tube for Re > Re crit Turbulent profile Velocity vectors unpredictably oscillating in time Time-averaged profile Much flatter profile than laminar flow Tendency for flattening grows with Re Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 50
51 Turbulent Flows Throughput according to Blasius ( ) Approximations well above 3Re * Laminar p l r Mean velocity p l r Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 51
52 Scaling of Mean Velocity Turbulent Laminar Pressure gradient Radius Density Viscosity Same pressure gradient applied to tube Smaller turbulent flow velocity Turbulent velocity varies with density Flow energy dissipated by turbulent mixing Laminar flow Viscous forces between smoothly sliding layers Turbulent regime Enhanced flow resistance Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 52
53 Entrance Effects Laminar Turbulent Microfluidic systems Re ~1 and r 0 = 100 µm z devel = 10 µm (laminar) Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 53
54 Surface roughness Local eddy formation Darcy-Weissbach relation Pressure loss Flow velocity Friction factor Friction Losses f = const. for smooth tube and laminar conditions Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 54
55 Roughness-Viscosity Model Surface roughness induces turbulence in boundary layer Surface roughness height Roughness viscosity Adding to bulk viscosity Surface roughness Reynolds number Empirical factor Microfluidics - Jens Ducrée Physics: Laminar and Turbulent Flow 55
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