Virtual Lab 5 Photosynthesis

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Gabriel York
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Name Period Assignment # Virtual Lab 5 Photosynthesis http://www.phschool.com/science/biology_place/labbench/lab4/intro.

1 Name Period Assignment # Virtual Lab 5 Photosynthesis 1) Define photosynthesis 2) Define chlorophyll Click 4-I Chromatography on the left side of the page 3) What is the paper chromatography used for? 4) What are the 3 factors that affect how far a molecule migrates up the paper? I. II. III. 5) Label the diagram below

2 6) Define pigment 7) Define chloroplast Click Next 8) What is going to carry the pigments found in chloroplasts up the paper? 9) Fill in the diagram below Click A Closer Look 10) Fill in the diagram below

3 Click Next 11) Fill in the diagram below Click Next 12) Why do we need to calculate the R f? 13) What is the equation for the R f value? 14) Calculate the R f value for the green ink from question #5. Show your work!

4 Click on Self Quiz 15) What is true about the chromatogram to the right? 16) Explain your answer to the question above 17) If a different solvent were used for the chlorophyll chromatography described earlier, what results would you expect? 18) Explain your answer to the question above 19) What is the R f value for carotene calculated from the chromatogram above? Show your work! Click 4-II Photosynthesis in the left side menu bar 20) Define pigment 21) Photons of light excite electrons found in what plant pigment? 22) What do the excited electrons do? 23) Define thylakoid 24) What captures the energy in the excited electrons?

5 Replay the animation a few times Click Next Concept 25) What is the role of DPIP in this experiment? 26) What molecule is DPIP replacing (go back to the previous animation if necessary)? 27) What happens to the color of DPIP when it is reduced (accepts electrons)? 28) Explain why color change in DPIP can be used to measure the rate of the light reactions of photosynthesis Click Next Concept 29) What is a spectrophotometer? 30) Explain how a spectrophotometer works

6 31) Fill in the diagram below

7 Click Next 32) What range do pigments absorb in? 33) What is the spectrophotometer going to measure? 34) Fill in the diagram below 35) Describe the color of DPIP when it is in its oxidized state 36) Will transmittance be high or low? 37) Excited electrons from the light reactions carried out in the thylakoid membranes of chloroplasts will reduce DPIP. Will transmittance be high or low if photosynthesis is high? 38) Explain your answer to the question above 39) What is in the blank 40) What is the purpose of having a blank?

41) Following the procedures described above, expose the tubes to light and make an initial spectrophotometer reading, followed by readings at 5, 10, and 15 minutes.

8 41) Following the procedures described above, expose the tubes to light and make an initial spectrophotometer reading, followed by readings at 5, 10, and 15 minutes. Print the graphs below and, based on your understanding of the light reactions of photosynthesis, draw in the approximate shapes of the curves you predict. Bring the graphs to class with you. Click Self Quiz 42) Which graph above would be the most likely result of performing the photosynthesis experiment using fresh chloroplasts placed in light and DPIP? 43) Explain your answer to the question above 44) What is the best explanation for graph B above? 45) Explain your answer to the question above

9 46) What effect would adding more DPIP to each experimental tube above have on these results? 47) Explain your answer to the question above 48) What is the role of DPIP in this experiment? 49) Explain your answer to the question above

A. PAPER CHROMATOGRAPHY

A. PAPER CHROMATOGRAPHY Period Date LAB. LAB BENCH PHOTOSYNTHESIS Because we are in a time crunch here toward the end of the year, we will not be able to do all the labs that I would like to fit in before the AP exam. But, since