Photosynthesis: Limiting Factors
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- Winifred Reed
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1 Name: Date: Period: Photosynthesis: Limiting Factors Purpose: 1. To determine the effect of temperature, light intensity, and light color on the rate of photosynthesis. 2. To calculate the rate of photosynthesis by measuring the number of oxygen bubbles produced in 30 second intervals. Directions: 1. The virtual lab can be accessed at the following site: 2. The lab set up is pictured below. Look at the diagram in order to know how to manipulate (change) the variables. A. Click on the thermometer to change Temperature of the water. B. Click on the CO 2 bottle to increase Or decrease the amount of CO 2 in The water. C. Click the green button to start and Stop the timer. Red button to reset. D. Click the orange button to change the color of the light bulb. E. Slide the circle across the bar to change the light intensity. The intensity will be seen as a percentage in the light bulb below. Experimental Design: 1. Based on the resources available, what are some possible variables that could be tested? 2. How many variables can you change in a single experiment? Explain your response. 3. What is our dependent variable? 4. Is the data being collected in the experiment qualitative or quantitative?
2 Number of Bubbles Produced Experiment 1: How does temperature affect the rate of photosynthesis? 2. Set the light intensity to 50%. 3. Start the timer by clicking the green button and count the number of bubbles that appear after 10 seconds. 4. Continue collecting data for one minute. Counting is continuous Do not start over. 5. Increase the temperature to 25 o by clicking on the thermometer and repeat step Increase the temperature to 40 o by clicking on the thermometer and repeat step o C 25 o C 40 o C The Effect of Temperature on Photosynthesis over Time Time (seconds)
3 Label: Experiment 2: How does light intensity affect the rate of photosynthesis? 2. Click on the thermometer to set the temperature to 25 o C 3. Set the light intensity to 15%. 4. Start the timer by clicking the green button and count the number of bubbles that appear after 10 seconds. 5. Continue collecting data for one minute. Counting is continuous Do not start over. 5. Increase the light intensity to 30% and repeat steps Increase the light intensity to 50% and repeat steps % light 30% light 50% light TITLE: Label:
4 Label: Experiment 3: How does the color of light affect the rate of photosynthesis? 2. Click on the thermometer to set the temperature to 25 o C 3. Set the light intensity to 50%. 4. Click the button on the light to change the bulb color to orange. 5. Start the timer by clicking the green button and count the number of bubbles that appear after 10 seconds. 6. Continue collecting data for one minute. Counting is continuous Do not start over. 7. Change the light color to green and repeat steps Change the light color to blue and repeat steps 5-6. Orange Green Blue TITLE: Label:
5 Analysis and Conclusion: Self-Quiz 1. What type of gas bubble are being produced? A. Carbon Dioxide B. Oxygen C. Nitrogen D. This cannot be determined 2. All of the following were used as independent variables in this activity EXCEPT: A. The number of oxygen bubbles B. Temperature C. Light Color D. Light Intensity 3. Which of the following best explains why carbon dioxide must be added to the water? A. Carbon dioxide bubbles can t be produced if it is not added to the solution B. Carbon dioxide maintains the green color seen in the plant C. Carbon dioxide is a needed reactant (input) of photosynthesis D. Carbon dioxide does not need to be added 4. Where would aquatic plants obtain the carbon dioxide needed to perform photosynthesis? A. Aquatic animals that exhale carbon dioxide. B. Other aquatic plants that produce carbon dioxide through photosynthesis C. Carbon dioxide diffuses into the water molecules D. Water breaks down into carbon dioxide and hydrogen 5. Which of the following best explains the effect of temperature on photosynthesis? A. Temperature does not affect the rate of photosynthesis B. Photosynthesis occurred at its highest rate at 10 o C C. Photosynthesis occurred at its highest rate at 25 o C D. Photosynthesis occurred at its highest rate at 40 o C 6. Which of the following best explains the effect of light intensity on photosynthesis? A. The lower the light intensity, the higher the rate of photosynthesis B. The intensity of light is inversely proportionate to the rate of photosynthesis C. The light intensity does not affect the rate of photosynthesis D. There is a direct relationship between light intensity and the rate of photosynthesis. 7. Which of the following best explains the effect of light color on photosynthesis? A. Blue light results in the highest rate of photosynthesis. B. Green light results in the highest rate of photosynthesis. C. Orange light results in the highest rate of photosynthesis. D. Both blue light and green light produce the highest rate of photosynthesis. 8. Which of the following scenarios would produce the highest rate of photosynthesis? A. Orange light at 30% intensity at a temperature of 40 o C B. Green light at 50% intensity at a temperature of 25 o C C. White light at 15% intensity at a temperature of 10 o C D. Blue light at 50% intensity at a temperature of 25 o C
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