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Gerard Fisher
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the data needed, and mpleting and reviewing the lletin f infrmatin.

1 RPRT DMNTATIN PAG Frm Apprved MB N The pbli reprting brden fr This lletin f infrmatin is estimated t average 1 hr per respnse, inlding the time fr reviewing instrtins, searhing existing data sres, gathering and maintaining the data needed, and mpleting and reviewing the lletin f infrmatin. Send mments regarding this brden estimate r any ther aspet f this lletin f infrmatin, inlding sggestins fr reding the brden, t the Department f Defense, xetive Servies and mmniatins Diretrate ( ). Respndents shld be aware that ntwithstanding any ther prvisin f law, n persn shall be sbjet t any penalty fr failing t mply with a lletin f infrmatin if it des nt display a rrently valid MB ntrl nmber PLAS D NT RTRN YR FRM T TH ABV RGANIZATIN. 1. RPRT DAT (DD-MM-YYYY) RPRT TYP Abstrats and ral Presentatins 4. TITL AND SBTITL Abstrats and ral Presentatins f: 16th Int. ngress f Marine rrsin & Fling (IMF) Jne 24-28, 2012 in Seattle, WA 3. DATS VRD (Frm - T) 5a. NTRAT NMBR 5b. GRANT NMBR 5. PRGRAM LMNT NMBR 6. ATHR(S) 5d. PRJT NMBR 5e. TASK NMBR 5f. WRK NIT NMBR 7. PRFRMING RGANIZATIN NAM(S) AND ADDRSS(S) Naval Researh Labratry eangraphy Divisin Stennis Spae enter, MS SPNSRING/MNITRING AGNY NAM(S) AND ADDRSS(S) ffie f Naval Researh ne Liberty enter 875 Nrth Randlph Street, Site 1425 Arlingtn, VA PRFRMING RGANIZATIN RPRT NMBR 10. SPNSR/MNITR'S ARNYM(S) NR 11. SPNSR/MNITRS RPRT NMBR(S) 12. DISTRIBTIN/AVAILABILITY STATMNT Apprved fr pbli release, distribtin is nlimited. 13. SPPLMNTARY NTS 14. ABSTRAT 15. SBJT TRMS 16. SRITY LASSIFIATIN F: a. RPRT b. ABSTRAT. THIS PAG nlassified nlassified nlassified 17. LIMITATIN F ABSTRAT 18. NMBR F PAGS 19a. NAM F RSPNSIBL PRSN 19b. TLPHN NMBR (Inlde area de) Standard Frm 298 (Rev. 8/98) Presribed by ANSI Std. Z39.18

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16 th Annal Internatinal ngress n Marine rrsin and Fling ABSTRAT SMMARIS MI-3 MILD STL RRSIN IN NARSHR MARIN NVIRNMNTS - ASSSSING TH PRSN F IRN-XIDIZING BATRIA AND HARATRIZING TH VRALL BATRIAL MMNITY

rg ('presenting athr) Little is knwn abt the mirbial elgy f rrding steel in marine envirnments 1-2 r f the natral abndane f irn-xidizing bateria (FeB) in these systems.

22 16 th Annal Internatinal ngress n Marine rrsin and Fling ABSTRAT SMMARIS MI-3 MILD STL RRSIN IN NARSHR MARIN NVIRNMNTS - ASSSSING TH PRSN F IRN-XIDIZING BATRIA AND HARATRIZING TH VRALL BATRIAL MMNITY Jye M. MBeth, David mersn* Bigelw Labratry fr ean Sienes, ast Bthbay, M SAjmbeth@bigelw.rg, demersn@bigelw.rg ('presenting athr) Little is knwn abt the mirbial elgy f rrding steel in marine envirnments 1-2 r f the natral abndane f irn-xidizing bateria (FeB) in these systems. We hypthesized that astal sediments are reservirs fr the marine FeB 'Zetaprtebateria' (Zetas), and that they an lnize and beme nmerially abndant n mild steel srfaes. A 40 day time series inbatin was ndted in a salt marsh (smmer 2010). rrsin mmnity DNA was extrated and analyzed fr baterial diversity with tagged pyrseqening (V4 regin, 16S rrna gene). Several relevant mmnities were qantified sing qpr: bateria and arhaea 3 and Zetas 4 sing 16S rrna gene speifi primers, and slfate-reding bateria (SRB) sing a dsra gene speifi primer 5. The pyrseqening data shwed the presene f Zetas in sediments and thrght the inbatins n the steel samples. Irn xyhydrxide stalk bisignatres were bserved n samples, frther evidene that these seqenes likely represent FeB. Relatives f the H2-xidizing gens Hydrgenphaga and members f the family Rhdbaterales were als identified as imprtant members f the birrsin mmnity and were present bth n steel and in sediments. Gene pies assessed with qpr remained fairly nstant in sediments dring the stdy, and Zetas were a 10-fld lwer than SRB. Zetas lnizing the steel inreased rapidly ver the first 10 days, exeeding pies qantified in the sediment by an rder f magnitde. The SRB nmbers n the steel were 10 fld lwer than in sediments dring the first days f inbatin, bt inreased t near the sediment levels by 40 days. This wrk illstrates that astal sediments are a reservir fr Zetas wh, thgh nmerially lw in sediments, an qikly lnize envirnments where free Fe(II) is abndant Referenes: (1) MBeth JM etal (2011) Appl nv Mirbil 77: ; (2) Dang H etal (2011) nv Mir 13: ; (3) Takai K & Hrikshi K (2000) Appl nv Mirbil 66: ; (4) Kat S etal (2009) nv Mirbil 11: ; (5) Ben- Dv etal (2007) Mirb l 54: MI-4 MARIN MI F MILD STL - LTRHMIAL ANALYSIS F MDL RRSIN MMNITIS Jye M. MBeth, David mersn Bigelw Labratry fr ean Sienes, ast Bthbay, M SAimbeth@bigelw.rg, demersn@bigelw.rg Jasn S. Lee, Rihard I. Ray, BrendaJ. Little* Naval Researh Labratry, Stennis Spae enter, MS, SA Jasn.Lee@nrlss.navy.mil, Riky.Ray@nrlss.navy.mil, 8renda.Little@nrlss.navy.mil ('rrespnding athr) Previs stdies have shwn that Fe(II)-xidizing bateria (FeB) and Fe(lII)-reding bateria (FeRB) are invlved in steel rrsin, and enhane mild steel rrsin in labratry stdies 12. The bjetive f this wrk was t determine the eletrhemistry f mild steel hallenged with single strains f FeB and FeRB vs ltres f FeB and FeRB. Bath experiments ntaining mild steel pns in marine medim were ndted in green flasks. Pre and mixed ltres f marine FeB (Mariprfünds ferrxydans strain M34) 3 and FeB (Gethermbater sp. strain HR-1) 4 were sed in eah system, and ntrls ntaining n added FeB and FeRB were als prepared. Pre FeB were grwn in an aerbi blk medim and pre FeRB were grwn nder anaerbi nditins. rrsin rates were mnitred eletrhemially, and fllwing inbatin, steel srfaes were evalated with SM and prfilmetry. An FeB and FeRB -ltre was sessflly grwn in an blk aerbi envirnment, and the FeB-generated irn xide stalks in this treatment appeared dended in mparisn with thse frmed in the pre FeB system. Prfilmetry demnstrated less nifrm rrsin attak in the presene f FeB and FeRB -ltre mpared t all ther expsres. letrhemially mnitred plarizatin resistane sggested that all aerbi rrsin rates were similar and rders-f-magnitde higher than anaerbi rrsin rates. Frther wrk develping mdel systems fr assessing the individal and lletive inflenes f key mirbes n rrsin inlde inrpratin f slfate-reding bateria. Referenes: (1) MBeth JM et al (2011) Appl nv Mirbil 77: ; (2) Herrera LK & Videla HA (2009) Int Bidet & Bideg 63: ; (3) MAllister SM etal (2011) Appl nv Mirbil 77: ; (4) mersn D (2009) Gemir J 26: I

REPORT DOCUMENTATION PAGE

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