HPLC Products. New 1.9μm Pinnacle DB Small Particle HPLC Columns. 2007/08 Edition. Chromatography Products. hplc columns.

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1 HPLC Products 2007/08 Edition hplc columns hplc accessories hplc replacement parts analytical reference materials vials & syringes New.9μm Pinnacle DB Small Particle HPLC Columns See page 8 for details. Chromatography Products

2 New.9μm Pinnacle DB Small Particle HPLC Columns See page 8 for details. We're At Your Service. customer service (ext. 3) Ordering Prices and Availability Delivery Information Order Status Return Authorizations (due to ordering errors) 2. technical service (ext. 4) support@restek.com Product Recommendations Applications Information Troubleshooting Solutions Custom Products Return Authorizations (due to technical issues) 3. literature request (ext. 5) also see our website For customer service or technical service outside the U.S.: please contact your local Restek representative. Ordering Information. CALL: (ext. 3) or (ext. 3) Monday Friday 8:00 a.m. 6:00 p.m. Eastern Time 2. FAX: hours a day 3. ON LINE: 24-hours a day To order from outside the U.S.: please contact your local Restek representative. Restek s payment terms are net 30 days. U.S. Terms FOB Bellefonte, PA Insurance and shipping costs are prepaid and added to invoice. Net due in 30 days,.5% late charge-per-month added after 30 days. For your convenience, we accept payment by MasterCard, Visa, or American Express. Prices and specifications subject to change without notice. Orders are subject to acceptance. Shipping Standard orders: UPS & Federal Express Rush orders: UPS Overnight or Federal Express Priority (Others available upon request.) If you do not receive your Air or Ground Track package on time, call our customer service department. We can track your package.

3 What's New HPLC Columns Small particle HPLC columns: our popular Pinnacle DB packings in.9µm particle size. Pages 8-9. Capillary HPLC columns for micro LC. Page. phidelity C8 columns: ph stable columns up to ph 2. Page 0. Allure Biphenyl columns: enhanced selectivity for unsaturated compounds. Now in both 3µm & 5µm particle sizes. Page 20. Allure AK columns: resolve 3 carbonyl compounds in 5 minutes. Page 2. New phases on Viva Wide Pore silica: Viva Biphenyl and Viva PFP Propyl HPLC columns, Restek manufactured wide pore Viva silica. Page 30. Restek is now your primary source for all Kromasil columns and packings. Pages HPLC Accessories Outlet cap, outlet cap & gold seal assembly, and connecting tubing for Agilent instruments. Page 4. Capillary assemblies for Agilent systems. Page 40. Syringes for CTC/Leap instruments. Page 65. Two-way, three-way, prime/purge, and check valves. Page 45. Restek Pack in a Box Kit: everything you need to pack your own analytical HPLC columns. Page 59. Table Of Contents HPLC Columns Bulk Packing Materials HPLC Guard Columns HPLC Replacement Parts and Accessories Technical Tips Vials & Syringes Analytical Reference Materials Applications Compound Index (Applications)

4 2 Column Selection Selecting an HPLC Column Choosing the best column for your application requires consideration of stationary phase chemistry, retention capacity, particle size, and column dimensions. Identifying the best stationary phase for your separation is the most critical step of column selection, and your decision should be based on sample solubility and the chemical differences among the compounds of interest. Figure is a handy tool for stationary phase selection. Becky Wittrig HPLC Products Marketing Manager 6+ years of service! Figure Stationary phase selection. Water or Polar Organics Sample Solubility Nonpolar Organics HPLC COLUMNS ordering note For assistance in selecting an HPLC column, please contact Restek Technical Service at or (ext. 4) or support@restek.com. Reversed Phase Acidic Ionic Aqueous C8 IBD Organic Acids Basic Amino Cyano Basix IBD PFP PFP Propyl Neutral Hydrophobic C C4 C8 C8 Phenyl Biphenyl Hydrophilic Aqueous C8 IBD Organic Acids Mixed Basix IBD Normal Phase Silica Cyano Amino IBD Free Literature HPLC Tech Tips Wall Chart Almost everything you need to remember about HPLC, condensed into 3 feet by 2 feet: mobile phase basics, buffers (types, pk a values, ph ranges, formula masses, more), miscibility and solubility chart (invaluable!), system setup and optimization, detector tips, pressure conversion factors, most-used chromatographic equations, column storage essentials. (lit. cat.# 59894A) Reversed phase columns (e.g. alkyl, phenyl, cyano) work well for water-soluble hydrophobic compounds. Some stationary phases incorporate both polar and nonpolar functionality and can be used in either reversed phase or normal phase modes (e.g. Ultra IBD, Allure Basix, and Allure PFP Propyl). While straight chain alkyl (e.g. C8) stationary phases are historically the most commonly used, many newer phases provide better separations. Alkyl phases are best suited for analyzing neutral compounds with a high ratio of carbon:heteroatoms where the major distinction among analytes is their hydrophobicity. However, for analyzing compounds that are highly polar, aromatic, or halogenated, nonalkyl stationary phases often provide significantly better selectivity (Figure 2). Retention capacity is another important consideration and is influenced by surface area and carbon load (% carbon in the packing material). Allure columns were designed for maximum retention using a high density of ligands bonded to a large surface area silica. Ultra, Kromasil, Pinnacle II and Pinnacle DB columns have the same high ligand density, but are more moderately retentive due to their lower surface areas. Surface area is inversely proportional to pore size; thus, larger pore sizes result in less retention. However, wide pore (e.g. 300Å) packings, such as Viva, are ideal when analyzing larger molecules, as a larger pore size is necessary to allow the analytes to fit into the pores. Particle size and column dimensions also influence column choice. In selecting a particle size, the primary consideration is efficiency (plates/meter) versus column pressure. A 3µm column will have approximately 50% more efficiency than a 5µm column, if all other conditions are constant for both columns. As particle size is further decreased (e.g. <2µm), theoretically, efficiencies will increase proportionally, based on the Van Deemter equation (and the usable flow rate range is much wider). Please note that column backpressure also increases as particle size decreases. Column dimensions include internal diameter and length, where the most commonly used internal diameter (ID) for HPLC columns is 4.6mm. In theory, resolution and pressure should be independent of column ID as long as flow rate is adjusted to maintain the same mobile phase linear velocity (flow rate is proportional to column cross-sectional area). Table I shows the approximate optimum flow rates for four column IDs. Table I Approximate optimum flow rates for various analytical column IDs. 5µm Particles 3µm Particles ID (mm) Optimum Flow Rate (ml/min.) Optimum Flow Rate (ml/min.)

5 Column Selection 3 Figure 2 Stationary phase comparison. Silica Surface Si Si Si Si Si Si Silica Surface CH3 Side Si Si Si Si P3 P2 Si CH2 Polar Group P C8 Alkyl phases (e.g. C8) Alkyl-based stationary phases, such as C8, are best suited for analyzing hydrophobic molecules with a high carbon:heteroatom ratio. Alkyl phase with polar functional group An alkyl-based stationary phase with either an embedded polar group (P), a polar side chain (P2), or a polar end-cap (P3), has significantly greater interaction with polar compounds than a traditional alkyl phase. HPLC COLUMNS Si Conventional Propyl Phenyl Si Biphenyl Phenyl & Biphenyl phases Phenyl stationary phases interact with compounds containing aromatic groups or unsaturated bonds through π-π interactions. The biphenyl stationary phase has even greater interaction due to the higher concentration of aromatic rings. Si Si Si Si Si Si Si Si Silica Surface Silica Surface Si Si F F F F F Pentafluorophenyl Propyl N C Cyano Cyano & Fluorinated phases Fluorinated phases (such as the pentafluorophenyl propyl (PFP propyl)) and cyano-based phases interact strongly with basic, nitrogen-containing and halogenated analytes. Si Si Si Si Si Si

6 4 Physical Characteristics of Restek HPLC Columns Restek End Pore Carbon HPLC Column Cap? Size (Å) load (%) Applications Pinnacle DB C8 Y 40 Hydrophobic C8 phase suitable for analyses of a wide range of compounds, from acidic through slightly basic. Pinnacle DB C8 Y 40 6 Pinnacle DB Cyano Y 40 4 Applications similar to Pinnacle DB C8, but with less hydrophobic retention. Less retention can be useful for shortening analysis time, if resolution is adequate. Suitable for a wide range of compounds, from acidic through slightly basic. Also useful for confirmation of analyses on a C8 or C8 column. Can be used in normal phase or reversed phase mode of separation. Pinnacle DB Phenyl Y Suitable for polar aromatic compounds, fatty acids, purines and pyrimidines. Pinnacle DB Silica NA 40 NA Normal phase mode of separation. Pinnacle II C8 Y 0 3 Superior general purpose C8 for non-basic analytes. HPLC COLUMNS Pinnacle II PAH Y 0 Maximum resolution of polynuclear aromatic hydrocarbons. Pinnacle II C8 Y 0 7 Superior general purpose C8 for non-basic analytes. Pinnacle II Cyano Y 0 4 Superior general purpose cyano for weakly-basic analytes. Used in either normal or reversed phase analyses. Pinnacle II Phenyl Y 0 6 Superior general purpose phenyl for neutral analytes. Pinnacle II Amino N 0 2 Excellent general purpose amino phase. Excellent choice for carbohydrate analysis. Pinnacle II Biphenyl Y 0 Multiple aromatic ring structures; excellent for explosives. Pinnacle II Silica NA 0 NA Ideal for polar analytes. Allure C8 Y Allure Aqueous C8 N 60 Ideal for MS and light-scattering detection of neutral to slightly polar solutes. Separates basic compounds, showing good deactivation; excellent for explosives or steroids. Ideal for analyses that require >90% water in the mobile phase. Excellent for highly water soluble or poorly organic soluble compounds. Excellent for water-soluble vitamins and organic acids. More retention than Ultra Aqueous columns. Allure AK Y 60 Ideal for the analysis of aldehydes and ketones as DNPH derivatives. Allure Basix Y 60 2 Ideal for LC/MS of basic solutes. Excellent for basic pharmaceuticals or other amine-containing compounds. Allure PFP Propyl Y 60 7 Ideal for MS, ELSD, or NPD detection of nucleosides, nucleotides, purines, pyrimidines, or halogenated compounds. Allure Organic Acids N 60 NA Excellent resolution of challenging organic acids. Allure Biphenyl Y Multiple ring structure; excellent for aromatic and unsaturated compounds. Increased retention over traditional phenyl phases. Allure Silica NA 60 NA Highly retentive phase for normal phase separation. Ultra C8 Y Ultra Aqueous C8 N 00 5 Ultra IBD N 00 2 Ideal for anilines, barbiturates, carbonyls, fat-soluble vitamins, fatty acids, glycerides, phthalates, PTH amino acids, steroids, other acids. Ideal for analyses that require >90% water in the mobile phase. Excellent for highly water soluble or poorly organic soluble compounds. Excellent for water-soluble vitamins and organic acids. A polar group assists in deactivating surface silanols and contributes to unique separation selectivities for acids, bases, zwitterions, and other polar compounds. Ultra C8 Y 00 2 Selectivity and peak shape similar to Ultra C8, but less hydrophobic retention. Ultra C4 Y 00 9 Ideal for peptides and small proteins. Ultra C NA 00 5 Alternative selectivity to Ultra C8 or C8 columns, especially for polar analytes. Shortest chain alkyl phase available for reversed phase separations. Ultra Cyano Y 00 8 Excellent for basic pharmaceuticals, steroids (normal or reversed phase conditions), or other basic compounds. Ultra Phenyl Y 00 0 Ideal for fatty acids, polynuclear aromatic hydrocarbons, purines and pyrimidines, and polar aromatics. Ultra Amino N 00 2 Superior general purpose amino phase. Ideal for carbohydrates. ph ranges and temperature limits: see product listings on pages listed here. Column lifetime will be shorter when operating at ph and/or temperature extremes.

7 Physical Characteristics of Restek HPLC Columns 5 Chromatographic Properties Similar Phases USP Code Page # Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Hypersil BDS C8, Zorbax Eclipse XDB-C8 L 2 Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Similar to Pinnacle DB C8, but the shorter alkyl chain provides less hydrophobic retention. Hypersil BDS C8 L7 2 Highly base-deactivated spherical silica manufactured by Restek. Cyano bonding. Hypersil BDS Cyano, Zorbax Eclipse XDB-CN L0 3 Highly base-deactivated spherical silica manufactured by Restek. Phenyl bonding. Hypersil BDS Phenyl, Zorbax Eclipse XDB-Phenyl L 3 Highly base-deactivated spherical silica manufactured by Restek. L3 3 Intermediate carbon load and surface area, suitable for a wide range of neutral to acidic compounds. Silica manufactured by Restek. Hypersil ODS, Spherisorb ODS L 4 Proprietary stationary phase; resolves 6 PAHs in US EPA Method 60. Silica manufactured by Restek. Unique 4 Provides shorter retention times for hydrophobic compounds than C8. Silica manufactured by Restek. Hypersil C8, Spherisorb C8 L7 5 More rugged than bare silica for normal phase analyses. Silica manufactured by Restek. Hypersil CPS, Spherisorb Cyano L0 5 Offers unique selectivity versus traditional alkyl chain phases, especially for aromatic compounds. Silica manufactured by Restek. Hypersil Phenyl, Spherisorb Phenyl L 6 Silica manufactured by Restek. Hypersil APS 2 Amino, Spherisorb Amino L8 6 Silica manufactured by Restek. Unique biphenyl phase. Unique L 7 Superior value phase for normal phase separation of polar analytes. Lower retention than Ultra C8. Silica manufactured by Restek. Hypersil Silica L3 7 Most retentive phase for hydrophobic and slightly polar analytes. Mobile phase containing higher percentage of organic modifier contributes to higher sensitivity in ESI-based LC/MS. Highly retentive and selective for reversed phase separations of polar analytes. Highly base deactivated. Compatible with highly aqueous (up to 00%) mobile phases. Highly retentive, highly selective phase, developed specifically for the analysis of aldehydes and ketones as DNPH derivatives. Ultracarb C8, BetaMax Neutral, Discovery C8 L 8 L 9 Unique 2 HPLC COLUMNS Highly retentive phase for analytes containing amino functionality. BetaMax Base, Maxsil CN L0 8 A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. Excellent for beta-blockers, halogenated compounds, nucleosides, nucleotides, pyridines, pyrimidines, tricyclic antidepressants. Discovery HS F5 9 Single 30cm column performs equally to two C8 columns in series. (AOAC Method 986.3) Unique 20 High purity, highly retentive phase for aromatic and unsaturated compounds. Unique L 20 High purity, highly retentive phase for normal phase separation of polar analytes. Very high surface area. Maxsil Si L3 2 A very retentive, high-purity phase that exhibits excellent peak shape for a wide range of compounds. Recommended as a general purpose reversed phase column. Highly retentive and selective for reversed phase separations of polar analytes. Highly base deactivated. Compatible with highly aqueous (up to 00%) mobile phases. One of a group of intrinsically base-deactivated (IBD) phases, with a polar group within, or intrinsic to, the alkyl bonded phase. Provides unique selectivity and high level of base deactivation while reducing or eliminating the need for mobile phase additives. Very retentive, high-purity, base-deactivated reversed phase packing that exhibits excellent peak shape for a wide range of compounds. Exceptionally stable C4 packing, with high bonding coverage and silanol base-deactivation. Exhibits shorter retention than C8 or C8 phases. Exceptionally stable C packing resists hydrolysis, even under acidic mobile phase conditions. Least retentive reversed phase hydrocarbon packing. High-purity cyano phase with reduced silanol activity. Often a better choice than C8 for basic pharmaceuticals. Cyano is the most stable bonded phase for normal phase mode. High-purity, highly retentive, base-deactivated phase with alternate selectivity to hydrocarbon phases, especially for aromatic analytes. Recommended for normal phase analyses of mono- and disaccharides and other similar compounds. Can also serve as a weak anion exchanger, with aqueous buffers. Discovery C8, Symmetry C8, Hypersil Gold C8, Luna C8, Zorbax C8, Kromasil C8, LiChrospher RP-8, Inertsil ODS-2, Develosil C8 AQUA C8, Aquasil C8, Hypersil Gold AQ, YMC ODS-Aq L 22 L 23 SymmetryShield, Discovery ABZ & ABZ+, Prism 22 Luna C8, Symmetry C8, Hypersil Gold C8 L7 23 Supelcosil Butyl (C4), Delta-Pak C4 L26 24 Spherisorb C L3 24 Platinum CN, Develosil Cyano, Luna CN, Hypersil Gold CN Platinum Phenyl, Supelcosil Phenyl, Betasil Phenyl L0 25 L 25 Platinum Amino, Develosil NH2 L8 26 Continued on next page...

8 6 Physical Characteristics of HPLC Columns; USP Descriptions Restek HPLC Column End Cap? Pore Size (Å) Carbon load (%) Applications Ultra PFP Y 00 7 Ideal for taxol and precursors, or halogenated compounds, amines, esters, or ketones. Ultra Silica NA 00 NA Ideal for normal phase applications. Ultra Carbamate 00 Rapid analysis of carbamates. Ultra Quat 00 Proprietary phase for the analysis of paraquat and diquat and other quaternary amines. Kromasil C8 Y HPLC COLUMNS Kromasil C8 Y 00 2 Kromasil C4 Y 00 8 Kromasil C Y 00 Kromasil Phenyl Y 00 4 Kromasil Amino Y 00.7% nitrogen Kromasil Silica 00 Viva Wide Pore C8 Y Proteins and other higher molecular weight compounds. Viva Wide Pore C8 Y Proteins and other higher molecular weight compounds. Less retentive than C8 phase. Viva Wide Pore C4 Y Proteins and other higher molecular weight compounds. Less retentive than C8 and C8 phases. Viva Wide Pore Biphenyl Viva Wide Pore PFP Propyl Y Exhibits excellent peak shape for a wide range of compounds; ideal for large molecule and biomolecule assays. Y Exhibits excellent peak shape for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds. Viva Wide Pore Silica 300 Normal phase applications for highly retained high molecular weight compounds. phidelity C8 NA 200 Hydrophobic C8 phase suitable for analyzing a wide range of compounds; enhanced stability under basic and acidic conditions. ph ranges and temperature limits: see product listings on pages listed here. Column lifetime will be shorter when operating at ph and/or temperature extremes. US Pharmacopoeia Cross Reference L L3 L7 L8 L0 L L3 L26 L43 Octadecyl silane chemically bonded to porous silica or ceramic microparticles,.7 to 0µm in diameter, or a monolithic rod. Allure C8 (p. 8), Pinnacle DB C8 (p. 2), Pinnacle II C8 (p. 4), Ultra C8 (p. 22), Allure Aqueous C8 (p. 9), Ultra Aqueous C8 (p. 23), Viva C8 (p. 29), Kromasil C8 (p. 32) Porous silica particles, 5 to 0µm in diameter. Allure Silica (p. 2), Pinnacle DB Silica (p. 3), Pinnacle II Silica (p. 7), Ultra Silica (p. 27), Viva Silica (p. 3), Kromasil Silica (p. 33) Octylsilane chemically bonded to totally porous silica particles,.7 to 0µm in diameter. Pinnacle DB C8 (p. 2), Pinnacle II C8 (p. 5), Ultra C8 (p. 22), Viva C8 (p. 29), Kromasil C8 (p. 33) An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 3 to 0µm in diameter. Pinnacle II Amino (p. 6), Ultra Amino (p. 26), Kromasil Amino (p. 33) Nitrile groups chemically bonded to porous silica particles, 3 to 0µm in diameter. Pinnacle DB Cyano (p. 3), Pinnacle II Cyano (p. 5), Ultra Cyano (p. 25), Allure Basix (p. 8) Phenyl groups chemically bonded to porous silica particles,.7 to 0µm in diameter. Pinnacle DB Phenyl (p. 3), Pinnacle II Phenyl (p. 6), Ultra Phenyl (p. 25), Allure Biphenyl (p. 20), Pinnacle II Biphenyl (p. 7), Viva Biphenyl (p. 30), Kromasil Phenyl (p. 33) Trimethylsilane chemically bonded to porous silica particles, 3 to 0µm in diameter. Ultra C (p. 24), Kromasil C (p. 33) Butyl silane chemically bonded to totally porous silica particles, 3 to 0µm in diameter. Ultra C4 (p.24), Viva C4 (p.30), Kromasil C4 (p.32) Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 5 to 0µm in diameter. Allure PFP Propyl (p. 9), Ultra PFP (p. 26), Viva PFP Propyl (p. 30)

9 Physical Characteristics of HPLC Columns; USP Descriptions 7 Chromatographic Properties A pentafluorophenyl phase. Unique selectivity by interaction with functional groups of organohalogens or other basic analytes. Similar Phases Fluophase PFP, Fluosep -RP Phenyl, Curosil PFP USP Code Page # L43 26 High purity, high surface area. L3 27 Proprietary stationary phase can process up to twice as many samples per hour, compared to a conventional C8 phase. Unique 27 High purity silica. Unique 28 High purity phase with excellent peak shape for a wide range of compounds. Good general pupose reversed phase column. Discovery C8, Symmetry C8, Hypersil Gold C8, Luna C8, Zorbax C8, LiChrospher RP-8, Inertsil ODS-2, Develosil C8 L 32 High purity, reversed phase packing for a wide range of compounds. Luna C8, Symmetry C8, Hypersil Gold C8 L7 33 High purity, reversed phase packing for a wide range of compounds. Less retentive than C8 and C8. Supelcosil Butyl (C4), Delta-Pak C4 L26 32 High purity, reversed phase packing for a wide range of compounds. Less retentive than C8, C8, and C4. Spherisorb C L3 33 High purity, base deactivated reversed phase packing. Alternate selectivity to alkyl phases. Platinum Phenyl, Supelcosil Phenyl, Betasil Phenyl L 33 High purity, base deactivated reversed phase packing. Alternate selectivity to alkyl phases. Platinum Amino, Develosil NH2 L8 33 High purity, base deactivated packing. L3 33 Silica manufactured by Restek. Silica manufactured by Restek. Silica manufactured by Restek. BioBasic 8, Symmetry 300 C8, Jupiter 300 C8, Zorbax 300 OSB C8, Synchropak C8, 208 TP C8 BioBasic 8, Zorbax 300 OSB C8, Synchropak C8, 208 TP C8 BioBasic 4, Symmetry 300 C4, Jupiter 300 C4, Synchropak C4, 208 TP C4 L 29 L7 29 L26 30 HPLC COLUMNS Silica manufactured by Restek. Unique L 30 Silica manufactured by Restek. Unique L43 30 Silica manufactured by Restek. L3 3 Excellent stability under extreme ph conditions. True C8 selectivity in a silica-based stationary phase. Unique 0 tech tip Managing High Backpressure High backpressure is one of the most common problems encountered in HPLC analyses. Normal column backpressure is observed after a new column has been installed and equilibrated with mobile phase. Unfortunately, this pressure often will increase as the column is used because particles collect on the column inlet frit. These particles can be sample impurities, mobile phase contaminants, or materials from the injector or autosampler rotor seal. In addition to increasing backpressure, particles on the frit can cause split peaks, peak tailing, and, eventually, over-pressure shut-down. In some circumstances, these problems can be corrected by back-flushing the column. However, in many cases the result is an unusable column. To minimize backpressure problems, all samples and mobile phase solvents must be filtered before use, and rotor seals should be changed on a routine basis. Along with these preventive measures, it is advisable to use precolumn filters such as the Trident guard column protection system. Particles build up on the inexpensive, replaceable frit in the filter, instead of on the permanent frit at the column inlet. Free Literature HPLC Column Selection Guide A useful chart to keep with your workbooks, or post on a wall. Quickly scan important characteristics of Restek HPLC columns. Includes brief, practical guidelines for choosing stationary phase, particle size, pore diameter, and column dimensions. Also includes USP designations for each phase and lists similar phases from other suppliers. (lit. cat.# 59454C)

10 8 New.9μm Pinnacle DB C8 Small Particle HPLC Columns free literature HPLC Small Particle Columns Call Restek at or , ext. 5, or contact your Restek representative, to request your free copy! lit. cat.# 58047A Restek s popular Pinnacle DB HPLC columns are now available in a <2μm particle size. Ruggedness and reproducibility are guaranteed, as we control every step in the process, from base silica to bonded phase to final packed column. The silica particles are classified and selected to give an exceptionally tight distribution around.9µm, while eliminating <µm particles that can contribute to a poorly packed bed. Highly base-deactivated Pinnacle DB stationary phases are an excellent choice when analyzing a wide range of compounds, from acidic to basic. To optimize your ultra high pressure HPLC methods, reach for Restek small particle HPLC columns!.9μm Pinnacle DB C8 HPLC Columns Physical Characteristics: particle size:.9µm endcap: yes pore size: 40Å ph range: carbon load: % temperature limit: 80 C Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. 2.mm ID Length cat.# price.9µm Columns 30mm mm mm Additional phases available June 2007! Visit or call Restek Technical Service for a full listing of our available phases.

11 Small Particle HPLC Columns 9 Figure Pinnacle DB silica particle size distribution chart shows an exceptionally tight, symmetrical distribution around.9μm with no <μm particles. Rick Lake Innovations Chemist 2+ years of service! HPLC COLUMNS Figure 2 Excellent peak symmetry and efficiency on a.9μm Pinnacle DB C8 column, using a reversed phase test mix.. uracil 3.00 mg/ml 2. benzene 0.02 mg/ml 3. naphthalene 0.50 mg/ml 4. biphenyl 0.06 mg/ml Performance: (calculations for biphenyl) Efficiency: 27,69 n/m Asymmetry:.0 Pressure: 4,500 psi Inj.: 2µL Sample diluent: water:methanol (25:75) Sample temp.: ambient Column: Pinnacle DB C8 Cat. #: Dimensions: 50 x 2.mm Particle size:.9µm Pore size: 40Å water:acetonitrile (45:55) 0.4 ml/min. Temp.: 25 C (or ambient) 254nm LC_EX0427

12 0 phidelity Columns phidelity C8 Columns Stable under extreme ph conditions. Patented technology protects the silica particle from dissolution. True C8 selectivity. HPLC COLUMNS Application Page # Basic Drugs Serotonin Reuptake Inhibitors Restek is pleased to offer phidelity C8 columns, designed for analyses that require extreme ph conditions. Using technology patented by Selerity Technologies (US Patent 2005/09503A), phidelity silica-based columns have exceptional stability under aggressive ph conditions. In phidelity columns, a polycarbosilane barrier layer protects the silica particle from extremely basic conditions. This layer, with multiple points of attachment to the silica particle, yields a modified surface with enhanced stability. A second layer is then attached, providing the functional group (C8). Using this approach to shield the silica surface, a highly durable stationary phase is created. Even after 50 hours of exposure to base (ph 0) at 60 C, a phidelity column maintains its original column efficiency. (A decrease in column efficiency indicates the dissolution of support silica and loss from the column.) This exceptional performance ensures phidelity columns offer greater resolution under harsh mobile phase conditions and will continue to resolve your target compounds over a long column lifetime. A phidelity column also maintains a very consistent capacity factor, k, after exposure to ph 0 and 60 C. Under extremely basic conditions, a decrease in retention time indicates loss of both support silica and bonded phase. Backpressure in the phidelity C8 column is very stable, indicating little or no degradation of the packing material. The patented process used to protect the support silica in phidelity columns ensures more consistent retention times over the lifetime of the column. phidelity C8 Columns Physical Characteristics: particle size: 3µm pore size: 40Å ph limit: up to 2 temperature limit: 80 C Chromatographic Properties: Excellent stability under extreme ph conditions. True C8 selectivity in a silica-based stationary phase. 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm mm ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

13 Capillary LC Columns Restek Capillary HPLC Columns High quality, Restek manufactured packing materials. Superior packing technology ensures rugged, reproducible columns. Wide range of phases and dimensions available please inquire. new! Bruce Albright HPLC Innovations Chemist 4+ years of service! Physical Characteristics: Allure Basix: particle size: 3µm, spherical pore size: 60Å carbon load: 2% endcap: fully endcapped ph range: 2.5 to 7.5 temperature limit: 80 C Allure Biphenyl: particle size: 3µm, spherical pore size: 60Å carbon load: 23% endcap: yes ph range: 2.5 to 7.5 temperature limit: 80 C Allure PFP Propyl: particle size: 3µm, spherical pore size: 60Å carbon load: 7% endcap: fully endcapped ph range: 2.5 to 7.5 temperature limit: 80 C Ultra Aqueous C8: particle size: 3µm, spherical pore size: 00Å endcap: no ph range: 2.5 to 7.5 temperature limit: 80 C Ultra C8: particle size: 3µm, spherical pore size: 00Å carbon load: 20% endcap: fully endcapped ph range: 2.5 to 7.5 temperature limit: 80 C Viva C8: particle size: 3µm, spherical pore size: 300Å carbon load: 9% endcap: yes ph range: 2.5 to 0 temperature limit: 80 C Chromatographic Properties: Available in a wide variety of stationary phases for acidic to basic compounds. Columns are suitable for small molecules using Allure and Ultra phases and large biomolecules using Viva C8. ordering note Looking for another stationary phase or capillary dimension? Please contact Restek Technical Service at or (ext. 4) or support@restek.com to inquire. HPLC COLUMNS 0.3mm ID Length cat.# price Allure Basix 3µm Capillary Columns 50mm 9635B 50mm 9636B Allure Biphenyl 3µm Capillary Columns 50mm 96635B 50mm 96636B Allure PFP Propyl 3µm Capillary Columns 50mm 96935B 50mm 96936B Ultra Aqueous C8 3µm Capillary Columns 50mm 97835B 50mm 97836B Ultra C8 3µm Capillary Columns 50mm 97435B 50mm 97436B Viva C8 3µm Capillary Columns 50mm 95435B 50mm 95436B

14 2 Pinnacle DB C8 and C8 Columns Pinnacle DB Columns: 3 or 5μm particle sizes; 40Å pore size Prepared using a highly base-deactivated silica support; ideal for analyses of basic compounds, or bases mixed with acids/neutrals. Silica manufactured at Restek, for total control of quality and reproducibility. HPLC COLUMNS Application Page # Vanilla Bean Extract Xanthines also available More chromatograms available! Visit or see our annual product catalog. Pinnacle DB C8 Columns (USP L) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: yes Silica pore size: 40Å ph range: carbon load: % temperature limit: 80 C Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Hydrophobic C8 phase suitable for analyses of a wide range of compounds, from acidic through slightly basic. Replaces Hypersil BDS C8 and Pinnacle ODS Amine..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Pinnacle DB C8 Columns (USP L7) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: yes Silica pore size: 40Å ph range: carbon load: 6% temperature limit: 80 C Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Similar to Pinnacle DB C8, but the shorter alkyl chain provides less hydrophobic retention. Less retention can be useful for shortening analysis time, if resolution is adequate. Replaces Hypersil BDS C8 and Pinnacle C8 Amine..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

15 Pinnacle DB Cyano, Phenyl, and Silica Columns 3 Pinnacle DB Cyano Columns (USP L0) Physical Characteristics: particle size: 5µm, spherical endcap: yes pore size: 40Å ph range: carbon load: 4% temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Cyano bonding. Suitable for analyses of a wide range of compounds, from acidic through slightly basic. Also useful for confirmation of analyses on a C8 or C8 column. Can be used in normal phase or reversed phase mode of separation. Replaces Hypersil BDS Cyano and Pinnacle Cyano Amine..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm Pinnacle DB Phenyl Columns (USP L) Physical Characteristics: particle range: 5µm, spherical endcap: yes pore size: 40Å ph range: carbon load: 5.3% temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Pinnacle DB Phenyl columns offer alternate selectivity to straight chain hydrocarbon phases, especially for aromatic analytes. Replaces Hypersil BDS Phenyl and Pinnacle Phenyl Amine. HPLC COLUMNS.0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm Pinnacle DB Silica Columns (USP L3) Physical Characteristics: particle size: 5µm, spherical endcap: no pore size: 40Å ph range: carbon load: none temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Useful for normal phase separations. Replaces Hypersil BDS and Pinnacle Amine..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm

16 4 Pinnacle II C8 and PAH Columns Pinnacle II Columns: 3μm or 5μm particles; 0Å pore size Silica manufactured at Restek, for total control of quality and reproducibility. Excellent replacement for the original Hypersil material. Physical and chromatographic properties similar to our original Pinnacle materials, but with greater lot-to-lot uniformity. HPLC COLUMNS Application Page # Allicin Capsaicinoids Morphine Sulfate Phenolic Antioxidants , 79 Pinnacle II C8 Columns (USP L) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: fully endcapped Silica pore size: 0Å ph range: 2.5 to 0 carbon load: 3% temperature limit: 80 C Chromatographic Properties: Excellent choice as a general purpose C8 column. Intermediate carbon loading and surface area, suitable for a wide range of acidic to neutral hydrophobic compounds. Replaces Hypersil ODS and Pinnacle C8..0mm ID 2.mm ID 3.2mm ID 4.0mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm new and Improved! Pinnacle II PAH Columns Physical Characteristics: particle size: 4µm, spherical pore size: 0Å endcap: fully endcapped ph range: 2.5 to 0 temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: Developed specifically for challenging analyses of polynuclear aromatic hydrocarbons. The Pinnacle II PAH stationary phase incorporates a proprietary C8 bonding that enables unique shape selectivity to resolve to baseline all 6 PAHs listed in US EPA Method 60. Every lot of Pinnacle II PAH bonded phase material is tested to ensure baseline resolution of the Method 60 PAHs, using a simple water/acetonitrile mobile phase gradient. Further, because we make Pinnacle II PAH columns using our own silica, we have greater control over quality and reproducibility. Replaces Pinnacle PAH columns. If you are analyzing PAHs, Pinnacle II PAH columns are the reliable, cost-effective columns you need. Application Page # PAHs mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 4µm Columns 50mm mm mm mm mm a plus story The Pinnacle II PAH column gives an excellent separation of the 8 target PAHs that we commonly analyze. Column lifetime far exceeded our expectations-after more than 5000 injections, the columns still maintain resolution and peak shape. Other PAH columns typically had lifetimes of 2000 to 2500 injections. We actually had one Pinnacle II PAH column last for more than 0,000 injections! Norm Farmer, Technical Director, Accutest Laboratories

17 Pinnacle II C8 and Cyano Columns 5 Pinnacle II C8 Columns (USP L7) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: fully endcapped Silica pore size: 0Å ph range: 2.5 to 0 carbon load: 7% temperature limit: 80 C Chromatographic Properties: Reliable performance and symmetrical peaks for neutral to acidic compounds. Provides shorter retention times for hydrophobic compounds, compared to C8 phases. Replaces Hypersil C8 and Pinnacle C8..0mm ID 2.mm ID 3.2mm ID 4.0mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Pinnacle II Cyano Columns (USP L0) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 0Å ph range: 2.5 to 7.5 carbon load: 4% temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: Can be used in either reversed phase or normal phase mode. More rugged than bare silica for normal phase applications. Replaces Hypersil Cyano and Pinnacle CN. Application Page # Parabens also available More chromatograms available! Visit or see our annual product catalog. HPLC COLUMNS.0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Application Page # Corticosteroids Piperine ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

18 6 Pinnacle II Phenyl and Amino Columns HPLC COLUMNS Application Page # Parabens Sorbic & Benzoic Acids also available More chromatograms available! Visit or see our annual product catalog. Application Page # Lactulose Concentrate Maple Syrup Sugars Pinnacle II Phenyl Columns (USP L) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 0Å ph range: 2.5 to 7.5 carbon load: 6% temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: The Pinnacle II Phenyl phase offers unique selectivity versus traditional alkyl chain phases, especially for aromatic compounds. Replaces Hypersil Phenyl and Pinnacle Phenyl..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Pinnacle II Amino Columns (USP L8) Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 0Å ph range: 2.5 to 7.5 carbon load: 2% temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: HPLC analysis using an amino-based stationary phase is the most popular technique for routine analyses of simple sugars, using isocratic elution (e.g., acetonitrile:water, 75:25) and a refractive index detector (RID) or an evaporative light scattering detector (ELSD). The Pinnacle II Amino column is ideal for mono- and disaccharide analyses. Replaces Hypersil Amino and Pinnacle Amino..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

19 Pinnacle II Biphenyl and Silica Columns 7 Pinnacle II Biphenyl Columns (USP L) Physical Characteristics: particle size: 5µm, spherical endcap: yes pore size: 0Å ph range: 2.5 to 7.5 temperature limit: 80 C Inj.: 0µL Conc.: 30µg/mL (30ppm) each Sample diluent: acetonitrile Sample temp.: ambient Standard: Cat. #: 3452, 3453, 3450, 345 Column: Pinnacle II Biphenyl Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å Temp.: water:methanol, 50:50, v/v.5 ml/min. ambient 254nm. HMX 2. RDX 3. nitrobenzene 4.,2-dinitrobenzene (SS) 5. 4-amino-2,6-dinitrotoluene 6. 2-amino-4,6-dinitrotoluene 7.,3-dinitrobenzene 8. 2-nitrotoluene 9a. 3-nitrotoluene 9b. 4-nitrotoluene 0. 2,6-dinitrotoluene. 3,4-dinitrotoluene (IS) 2.,3,5-trinitrobenzene 3. 2,4-dinitrotoluene 4. tetryl 5. 2,4,6-trinitrotoluene Restek Manufactured Silica Chromatographic Properties: The Pinnacle II Biphenyl phase offers alternate selectivity to straight-chain hydrocarbon phases, and enhanced selectivity and retention for unsaturated compounds, compared to traditional phenyl phases. An excellent confirmation column for explosive compounds, as in EPA method mm ID Length cat.# price 5µm Column 50mm mm Explosives by US EPA Method 8330 on a Pinnacle II Biphenyl column. Application Page # Explosives , 70 HPLC COLUMNS LC_0322 Pinnacle II Silica Columns (USP L3) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: no Silica pore size: 0Å ph range: 2.5 to 0 carbon load: none temperature limit: 80 C Chromatographic Properties: Good general purpose packing for normal phase separations. Moderate surface area. Replaces Hypersil and Pinnacle Silica..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Application Page # Hydrocodone Bitartrate Tocopherols

20 8 Allure C8 and Basix Columns Allure Columns: 3μm or 5μm particles; 60Å pore size Small pore size in a high-purity, Type B silica provides a large surface area. High carbon loads, highly retentive. An excellent choice for light scattering (ELSD) and MS detectors, in which more organic solvent in the mobile phase gives better sensitivity. HPLC COLUMNS Application Page # Antibiotics Herbicides Pesticides also available More chromatograms available! Visit or see our annual product catalog. Allure C8 Columns (USP L) Excellent Columns for LC/MS and ELSD Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 60Å ph range: 2.5 to 7.5 carbon load: 27% temperature limit: 80 C Chromatographic Properties: Most retentive of our alkyl stationary phases due to large surface area of the base silica and high-density bondings. Provides excellent peak shapes for a wide range of compounds..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Application Page # Acetaminophen/Narcotic Analgesics Acetaminophen, Pseudoephedrine & Caffeine Antiarrhythmics , 87 Antidepressants Albuterol Herbicides Ibuprofen & Pseudoephedrine Sedatives Steroid Hormones Sulfamethoxazole Allure Basix Columns (USP L0) Excellent Columns for LC/MS and ELSD Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 60Å ph range: 2.5 to 7.5 carbon load: 2% temperature limit: 80 C Chromatographic Properties: Highly retentive propyl cyano phase. Excellent choice for basic compounds and for analytes containing amine group functionality..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm

21 Allure PFP Propyl and Aqueous C8 Columns 9 Allure PFP Propyl Columns (USP L43) Excellent Columns for LC/MS and ELSD Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 60Å ph range: 2.5 to 7.5 carbon load: 7% temperature limit: 80 C Chromatographic Properties: A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. An excellent phase for separating nucleosides, nucleotides, purines, pyrimidines, halogenated compounds, β-blockers, and tricyclic antidepressants. Application Page # Catecholamines Cocaine/Ecgonine Methyl Ester Nucleic Acid Bases Pesticides mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm HPLC COLUMNS Allure Aqueous C8 Columns (USP L) Excellent Columns for LC/MS and ELSD Physical Characteristics: particle size: 5µm spherical endcap: no pore size: 60Å ph range: 2.5 to 7.5 temperature limit: 80 C Chromatographic Properties: Highly retentive and selective phase for separating polar analytes, including polar acidic compounds. Compatible with highly aqueous (up to 00%) mobile phases. Highly base deactivated. An excellent choice when analyzing a wide range of compounds, as in LC/MS screening methods..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

22 20 Allure Biphenyl and Organic Acids Columns HPLC COLUMNS Application Page # Corticosteroids Steroid Hormones Steroids (Contraceptive)...93 Tetracyclines free literature Allure Biphenyl HPLC Columns Flyer Analyses of corticosteroids, contraceptive steroids, and endogenous steroid hormones show the unique separation mechanism of the Allure Biphenyl phase - pi-pi interactions - provides markedly better selectivity, relative to a C8 or phenyl phase, for molecules that differ in the number or positions of multiple bonds. For saturated analytes the Allure Biphenyl phase has retention and selectivity similar to a C8 phase, but superior to a phenyl phase. Download your free copy from! lit. cat.# Application Page # Carboxylic Acids Fruit Juice Acids Allure Biphenyl Columns (USP L) Physical Characteristics: new! particle size: 3µm or 5µm, spherical endcap: yes pore size: 60Å ph range: 2.5 to 7.5 carbon load: 23% temperature limit: 80 C Chromatographic Properties: Highly retentive and selective for aromatic and unsaturated compounds. Increased retention and selectivity, compared to phenyl phases. Excellent selectivity for steroids, tetracyclines, explosives, and other unsaturated compounds..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Allure Organic Acids Columns Physical Characteristics: particle size: 5µm, spherical pore size: 60Å Chromatographic Properties: Allure Organic Acids columns provide enhanced retention and selectivity for polar organic acids, allowing the separation to be performed on a single 30cm column. An Allure Organic Acids column effectively resolves key organic acids such as tartaric and quinic acids, using the chromatographic conditions specified in AOAC method Retention is stable and reproducible, even with the 00% aqueous mobile phase specified in the AOAC method. Note: Other dimensions available on request. endcap: no ph range: 2.5 to 7.5 temperature limit: 80 C 3.2mm ID 4.6mm ID Length cat.# price cat.# price 5µm Column 50mm mm mm ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

23 Allure Silica and AK Columns 2 Allure Silica Columns (USP L3) Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 60Å endcap: no ph range: 2.5 to 7.5 temperature limit: 80 C Chromatographic Properties: Highly retentive phase for normal phase separations. Very high surface area, Type B silica packing..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Allure AK Columns Physical Characteristics: particle size: 5µm pore size: 60Å endcap: yes ph range: temperature limit: 80 C new! Chromatographic Properties: This highly retentive, highly selective phase, unique to Restek, was developed specifically for the analysis of aldehydes and ketones as DNPH derivatives. Allure AK is a reversed phase HPLC material that has the unique ability to separate all thirteen carbonyl compounds specified in California Air Resources Board (CARB) Method # 004, using a simple acetonitrile/water gradient, in less than 5 minutes. Other columns require long analysis times or the use of tetrahydrofuran. Application Page # Carbonyls , 69 HPLC COLUMNS 3.2mm ID 4.6mm ID Length cat.# price cat.# price 5µm Columns with Trident Integral Inlet Fittings 200mm Carbonyls by CARB Method 004 on an Allure AK column. dinitrophenylhydrazine (DNPH) derivatives of aldehydes/ketones Inj.: 0µL Conc.: 3µg/mL each analyte, as aldehyde/ketone Sample diluent: acetonitrile Column: Allure AK Cat.#: Dimensions: 200 x 4.6mm Pore size: 60Å A) water : B):acetonitrile Time (min.) %B mL/min. Temp.: 30 C 360nm Ret. Time Peak (min.) DNPH derivative of:. formaldehyde acetaldehyde acrolein acetone propionaldehyde crotonaldehyde methacrolein butyraldehyde methylethylketone benzaldehyde valeraldehyde m-tolualdehyde hexaldehyde.36 Frank Dorman Director of Technical Development + years of service! LC_EV0393

24 22 Ultra C8 and C8 Columns Ultra Columns: 3μm or 5μm particles; 00Å pore size Our broadest selection of stationary phases, including unique phases. High density bondings, for maximum retention. High-purity, Type B silica gives excellent peak shapes for a wide range of compounds. HPLC COLUMNS Application Page # Acetaminophen/Narcotic Analgesics Aldehydes/Ketones Beclomethasone Corticosteroids Explosives Herbicides Hydrocodone Bitartrate/ Acetaminophen Nitrofuran Metabolites Vitamins (Fat Soluble) Ultra C8 Columns (USP L) Excellent for a wide range of analyses Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 20% temperature limit: 80 C Chromatographic Properties: A retentive, high-purity packing that exhibits excellent peak shape for a wide range of compounds. Excellent general-purpose reversed phase column..0mm ID 2.mm ID 3.2mm ID 4.0mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Application Page # Oxycodone Vanillin & Ethyl Vanillin Heather Johnson Special Events Coordinator 0+ years of service! Ultra C8 Columns (USP L7) Excellent for a wide range of analyses Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 2% temperature limit: 80 C Chromatographic Properties: A retentive, high-purity, base-deactivated reversed phase packing that exhibits excellent peak shape for a wide range of compounds. Less retention for neutral, hydrophobic compounds, compared to the Ultra C8 column..0mm ID 2.mm ID 3.2mm ID 4.0mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm

25 Ultra Aqueous C8 and IBD Columns 23 Ultra Aqueous C8 Columns (USP L) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: no pore size: 00Å ph range: 2.5 to 7.5 temperature limit: 80 C Chromatographic Properties: Highly retentive and selective for reversed phase separations of polar analytes. Highly base-deactivated. Compatible with highly aqueous (up to 00%) mobile phases..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Application Page # Amino Acids (Aromatics)...67 Analgesics Carboxylic Acids Herbicides , 72 Phenethyl Glucosinolate...77 Sudan Dyes also available More chromatograms available! Visit or see our annual product catalog. HPLC COLUMNS Ultra IBD Columns Specialized Columns for Mixed Polar and Nonpolar Compounds Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: no pore size: 00Å ph range: 2.5 to 7.5 carbon load: 2% temperature limit: 80 C Chromatographic Properties: An intrinsically base-deactivated (IBD) phase, containing a polar group within, or intrinsic to, the hydrocarbon bonded phase. Unique selectivity and a high level of base deactivation, while reducing or eliminating the need for mobile phase additives..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Application Page # Antiarrhythmics Antibiotics , 89 Cimetidine Diflubenzuron (Pesticide)..7 Glyburide Nucleosides, Nucleotides & Nucleic Acid Bases Vitamins (Water Soluble)...78 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

26 24 Ultra C4 and C Columns Ultra C4 Columns (USP L26) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 9% temperature limit: 80 C Chromatographic Properties: Exceptionally stable C4 packing, with high bonding coverage and base deactivation. Less retention than C8 or C8 phases. HPLC COLUMNS also available More chromatograms available! Visit or see our annual product catalog..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Ultra C Columns (USP L3) Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 00Å ph range: 2.5 to 7.5 carbon load: 5% temperature limit: 80 C Chromatographic Properties: Exceptionally stable C phase. Least retentive reversed phase hydrocarbon packing..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

27 Ultra Cyano and Phenyl Columns 25 Ultra Cyano Columns (USP L0) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 8% temperature limit: 80 C Chromatographic Properties: High-purity cyano phase with few silanol sites. Often a better choice than C8 phases for basic pharmaceuticals, especially regarding peak shape and selectivity. Cyano phases are more rugged than bare silica for normal phase analyses because they are less sensitive to small amounts of water in the mobile phase. Application Page # Antiarrhythmics mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm HPLC COLUMNS Ultra Phenyl Columns (USP L) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 0% temperature limit: 80 C Chromatographic Properties: High-purity, highly retentive, base-deactivated phase with alternative selectivity to straight chain hydrocarbon phases, especially for aromatic analytes. Application Page # Guaifenesin/Codeine mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm a plus story After the disaster of 9/, Diazald, a highly explosive compound used in herbicide analysis, was immediately controlled by the U.S. government which made shipment impossible. Restek was instrumental in helping me to develop an isocratic HPLC method that did not require the use of Diazald. This method is not only safer, but it saves us time and money. Thanks, Restek! Chris Domaradzki, Organics Manager, Environmental Testing Laboratories Vernon Bartlett HPLC Senior Innovations Chemist 9+ years of service!

28 26 Ultra Amino and PFP Columns HPLC COLUMNS also available More chromatograms available! Visit or see our annual product catalog. Ultra Amino Columns (USP L8) Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 00Å ph range: 2.5 to 7.5 carbon load: 2% temperature limit: 80 C Chromatographic Properties: Recommended for normal phase analyses of mono- and disaccharides, or similar compounds..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm Application Page # Digitalis Extract Levothyroxine Purines/Pyrimidines Randy Romesberg HPLC Senior Innovations Chemist 2+ years of service! Ultra PFP Columns (USP L43) Unique Selectivity Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 7% temperature limit: 80 C Chromatographic Properties: A pentafluorophenyl phase. Unique selectivity for compounds containing organohalogens or other basic functional groups..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

29 Ultra Silica and Carbamate Columns 27 Ultra Silica Columns (USP L3) Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 00Å carbon load: none Chromatographic Properties: High surface area, Type B silica packing. endcap: no ph range: 2.5 to 7.5 temperature limit: 80 C.0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm µm Columns 30mm mm mm mm mm mm HPLC COLUMNS Ultra Carbamate Columns Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 00Å ph range: 2.5 to 7.5 temperature limit: 80 C restek exclusive! Application Page # Pesticides (Carbamates)...73 Chromatographic Properties: Restek chemists developed the Ultra Carbamate column specifically for carbamates analysis. The unique packing separates 0 target carbamates in just over 0 minutes. The column is compatible with fluorescence or LC/MS detection.* An Ultra Carbamate column can process as many as 3 to 4 samples per hour, versus less than 2 samples per hour on a general-purpose C8 column. In addition to increased sample throughput, this much faster analysis will significantly reduce solvent usage and the costs of disposing of solvent waste..0mm ID 2.mm ID 3.2mm ID 4.0mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price cat.# price 3µm Columns 50mm mm µm Columns 250mm *For post-column derivatization / fluorescence detection applications for a 4.6mm ID column the total system dead volume, including the post-column reactor, must be less than 650µL. For standard post-column reactor systems, we recommend a 250 x 4.6mm, 5µm column. Call Restek technical service for more information. restek innovation! Faster analyses and reduced solvent use! An Ultra Carbamate column can process as many as 3 to 4 samples per hour, versus less than 2 samples per hour on a general-purpose C8 column. also available We also have syringe filters! See

30 28 Ultra Quat Columns HPLC COLUMNS restek innovation! An Ultra Quat column and Ultra Quat Reagent Solution eliminate the need for ion pairing reagents in paraquat/diquat analysis. Application Page # Paraquat/Diquat , 75 for more info For more information about the Ultra Quat system, review Restek Advantage 2004 vol. 3 at. Ultra Quat Columns Physical Characteristics: particle size: 5µm, spherical pore size: 00Å Ultra Quat Reagent Solution Use with Ultra Quat HPLC column. Dilute to liter mobile phase, per instructions. In water, 20mL/bottle cat. # 3244 (ea.) ph range: 2.5 to 7.5 temperature limit: 80 C Paraquat & Diquat Calibration Mix diquat dibromide paraquat dichloride,000µg/ml each in water, ml/ampul cat. # (ea.) Consistent resolution, retention times, and peak symmetry for paraquat and diquat on an Ultra Quat column. 2 restek exclusive! Chromatographic Properties: A retentive, high-purity, base deactivated reversed phase packing. Ideal for the analysis of paraquat and diquat or other quaternary amines when used with Ultra Quat Reagent Solution mobile phase additive (cat. # 3244). 4.6mm ID Length cat.# price 5µm Column 50mm free literature Peak time (min.). diquat paraquat nm 257 nm Simple, Sensitive HPLC/UV Analysis for Paraquat and Diquat These highly charged quaternary amines are poorly retained on alkyl stationary phases. Using only acetonitrile, water, and a solvationblocking reagent, our separation system alters the interactions among analyte, mobile phase, and stationary phase, and promotes solubility of the analytes in the stationary phase. In our system, the detection limit is 6ppb for either herbicide, and the analysis is completed in less than 0 minutes. An optimized solid phase extraction cartridge concentrates the herbicides for the analysis. Inj.: 20µL Conc.: 20ppm each component (above); 20µg/mL, 40µg/mL, and 00µg/mL each component at 308nm (left) and at 257nm (right) Sample diluent: water Column: Ultra Quat Cat. #: Dimensions: 50 x 4.6mm Temp.: 27 C Ultra Quat Reagent Solution (cat.# 3244): acetonitrile, 95:5 (v/v).0 ml/min. 257nm (paraquat) 308nm (diquat) 2 min. Call Restek at or , ext. 5, or contact your Restek representative, to request your free copy! 308 nm 257 nm LC_032 min. lit. cat.#

31 Viva C8 and C8 Columns 29 Viva Wide Pore HPLC Columns: 3μm or 5μm particles; 300Å pore size Excellent for separating peptides or proteins. Rugged, spherical particles, with 300Å pore size. High proportion of pore/surface area available to large molecules. Viva columns are based on a wide pore material we designed for optimal large molecule separations. In developing Viva silica, we found that although many commercial wide-pore silicas meet the standard 300Å mean pore size, most have very broad distributions about this mean, with a significant portion of their pore volume falling below 50Å. This means a large portion of the surface area is unavailable to larger molecules. Viva columns have a narrow distribution about the mean pore size, permitting a larger portion of the silica surface to play a role in the separation. Viva C8 Columns (USP L) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: yes Silica pore size: 300Å ph range: 2.5 to 0 carbon load: 9% temperature limit: 80 C Chromatographic Properties: Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds. Excellent general-purpose column for analyzing large molecules and biomolecules..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm mm mm mm µm Columns 30mm mm mm mm mm mm restek innovation! Viva silica has a narrow distribution about the mean pore size, permitting a larger portion of the silica surface to play a role in the separation of large molecules and biomolecules. Application Page # Peptides Proteins new! ordering note If you need another column dimension or phase, please call for a quote. HPLC COLUMNS Viva C8 Columns (USP L7) Physical Characteristics: Restek Manufactured particle size: 5µm, spherical endcap: yes Silica pore size: 300Å ph range: 2.5 to 7.5 carbon load: 5% temperature limit: 80 C Chromatographic Properties: Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds. Less retention in reversed phase assays than Viva C8..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39. also available 3µm particles are available for all Viva phases please inquire. Tawnya Childs Call Center Manager 0+ years of service!

32 30 Viva C4, Biphenyl and PFP Propyl Columns HPLC COLUMNS Tim Herring Technical Service Specialist 3+ years of service! for more info For more information, request our Viva Wide Pore HPLC Columns flyer. lit. cat# also available 3µm particles are available for all Viva phases please inquire. Viva C4 Columns (USP L26) Physical Characteristics: Restek Manufactured particle size: 5µm, spherical endcap: yes Silica pore size: 300Å ph range: 2.5 to 7.5 carbon load: 3.5% temperature limit: 80 C Chromatographic Properties: Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds. Less retention in reversed phase assays than Viva C8 or Viva C8..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm new! Viva Biphenyl Columns (USP L) Physical Characteristics: particle size: 5µm pore size: 300Å carbon load: 6.7% endcap: yes ph range: 2.5 to 7.5 temperature limit: 80 C restek exclusive! Chromatographic Properties: Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds; ideal for large molecule and biomolecule assays. Highly retentive and selective phase for aromatic and unsaturated compounds, with increased retention, relative to phenyl phases..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm new! Viva PFP Propyl Columns (USP L43) Physical Characteristics: particle size: 5µm, spherical pore size: 300Å carbon load: 5% endcap: yes ph range: 2.5 to 7.5 temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm

33 Viva Silica Columns (USP L3) Physical Characteristics: particle size: 5µm, spherical pore size: 300Å ph range: 2.5 to 7.5 temperature limit: 80 C Viva Silica Columns; Fast LC 3 Restek Manufactured Silica Chromatographic Properties: Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds in normal phase separations..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39. HPLC COLUMNS Fast LC Method Development Kits Fast, efficient separations in an economical cartridge format. The design allows rapid substitution of one cartridge for another. The holder is available with either standard fittings or with a universal PEEK tip fitting. The universal fitting gives the user the option of threading the column directly into the detector inlet, reducing system dwell volume. The cartridges are 30mm long, with either 2.mm or 4.0mm ID. The 2.mm and 4.0mm ID cartridges have the same outside diameter, so the holder will accept either. Each kit includes four 3µm Fast LC Cartridges: Ultra C8 (USP L), Ultra Aqueous C8 (USP L), Ultra Cyano (USP L0), Ultra PFP (USP L43), and a Fast LC cartridge holder. Fast LC Development Kits Description qty. cat.# price Fast LC Development Kit, four 30 x 2.mm columns kit Fast LC Development Kit, four 30 x 4.0mm columns kit μm Fast LC Cartridges Description Length 2.mm ID 4.0mm ID price Ultra C8 Fast LC Cartridge 30mm Ultra Aqueous C8 Fast LC Cartridge 30mm Ultra Cyano Fast LC Cartridge 30mm Ultra PFP Fast LC Cartridge 30mm Fast LC Cartridge Holders Description qty. cat.# price Fast LC Cartridge Holder with PEEK Tip ea Fast LC Cartridge Holder with Standard Fittings ea Fast LC saves time and conserves solvent. also available Additional column kits and Fast LC cartridges are available. Please call for more information.

34 32 Kromasil Columns Kromasil Chromatography Products Perfectly spherical, totally porous HPLC silica products from Kromasil. Wide range of bonded phases and particle sizes. Excellent chemical purity and stability, for highly efficient, reproducible separations. Kromasil HPLC silica products consist of highly spherical, porous particles in sizes from 3.5µm to 6µm and larger. The surface properties of the silica have been optimized, and include a narrow pore size distribution and a well-defined pore structure. For chromatographic separations, these properties ensure higher efficiencies, lower pressure drops, and excellent lot-to-lot reproducibility. HPLC COLUMNS Table I Typical chemical purity for Kromasil spherical silicas. Content Metal (ppm) Na <20 Al <0 Fe <0 Values based on AAS or ICP measurements. Kromasil spherical silicas are produced using a sol-gel technique, which yields a mechanically strong particle with a large surface area (330m 2 /g for 00Å silica). Metal impurities are carefully monitored, as trace metals in the silica structure increase the surface acidity and can lead to tailing peaks for basic or chelating compounds. Typical metal content for Kromasil silicas is shown in Table I. Kromasil bonded phases are manufactured using monofunctional silanes, for higher surface coverage and greater reproducibility. Bonding is followed by an end-capping procedure, which further increases chemical stability. Restek offers all Kromasil phases, in a full range of column dimensions and silica particle sizes. A sampling of our product offering is shown here. For further information about Kromasil products available from Restek, please contact Restek technical service at , ext. 4, , ext. 4, or support@restek.com Kromasil HPLC Columns Physical Characteristics: particle size: 3.5µm or 5µm, spherical pore size: 00Å endcap: fully endcapped ph range:.5 to 0 also available For guard cartridges for these columns, see page 39. Kromasil C8 Columns (USP L) average carbon load: 20% 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 3.5µm Columns 30mm mm mm mm µm Columns 30mm mm mm mm mm mm Kromasil C4 Columns (USP L26) average carbon load: 8% 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm

35 Kromasil Columns 33 Kromasil C8 Columns (USP L7) average carbon load: 2% 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 3.5µm Columns 30mm mm mm mm µm Columns 30mm mm mm mm mm mm Kromasil C Columns (USP L3) 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm Kromasil Phenyl Columns (USP L) average carbon load: 4% 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 5µm Columns 50mm mm mm Sean Nolan Materials Manager 7+ years of service! also available Other Kromasil column dimensions, phases, and particle sizes are available. Please contact Technical Service at or , ext. 4, to inquire. HPLC COLUMNS Kromasil Amino Columns (USP L8) nitrogen load:.7% 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 5µm Columns 50mm mm mm Kromasil Silica Columns (USP L3) 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 5µm Columns 30mm mm mm mm mm mm free literature Kromasil Columns & Bulk Packings Call Restek at or , ext. 5, or contact your Restek representative, to request your free copy! lit. cat.#

36 34 Prep Columns HPLC Prep Columns Easy scale-up from Restek analytical columns. Popular bonded phases. Restek makes it easy to scale up your separations. We offer a wide range of semi-preparative and preparative-scale columns packed with many of our popular Ultra, Allure, Pinnacle DB, and Pinnacle II bonded phases on a 5µm spherical silica. Other stationary phases and particle sizes are available if you need a phase on 7µm, 0µm or 5µm particles, please call. HPLC COLUMNS Supersize without surprise! ordering note We strongly recommend ordering a semi-prep or prep column only after scouting the desired separation on an equivalent analytical-scale column. Because we cannot re-use a column or the silica it contains once it has left our facility, we cannot accept returns of large-scale columns (except in cases of our error). Pinnacle DB HPLC Prep Columns Dimensions Pinnacle DB C8 Pinnacle DB C8 Pinnacle DB Cyano Pinnacle DB Silica Length x ID cat.# cat.# cat.# cat.# price 50 x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm Pinnacle II HPLC Prep Columns Dimensions Pinnacle II C8 Pinnacle II C8 Pinnacle II Cyano Pinnacle II Silica Length x ID cat.# cat.# cat.# cat.# price 50 x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm

37 Prep Columns 35 Allure HPLC Prep Columns Dimensions Allure C8 Allure Basix Allure PFP Propyl Allure Silica Length x ID cat.# cat.# cat.# cat.# price 50 x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm Ultra HPLC Prep Columns HPLC COLUMNS Dimensions Ultra C8 Ultra Aqueous C8 Ultra C8 Ultra IBD Ultra Cyano Ultra Phenyl Ultra PFP Ultra Silica Length x ID cat.# cat.# cat.# cat.# cat.# cat.# cat.# cat.# price 50 x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm x 0mm x 2.2mm x 30mm x 50mm ordering note We strongly recommend ordering a semi-prep or prep column only after scouting the desired separation on an equivalent analytical-scale column. Because we cannot re-use a column or the silica it contains once it has left our facility, we cannot accept returns of large-scale columns (except in cases of our error). Kromasil Prep Columns Dimensions Kromasil C8 Kromasil C8 Length x ID cat.# cat.# price 50 x 0mm x 2.2mm x 30mm x 0mm x 2.2mm x 30mm x 0mm x 2.2mm x 30mm new!

38 36 Bulk Packing Materials Bulk Packing Materials Use our bulk packing materials to pack your own columns! Restek is among the small group of column manufacturers capable of producing their own high purity silica. We offer our Pinnacle II and Pinnacle DB silica and bonded phases in bulk. Pinnacle II is an excellent replacement for Hypersil silica. Pinnacle DB is a highly base-deactivated material for analyses of basic compounds and an excellent alternative to Hypersil BDS silica. Bonded phases from our Ultra, Allure, and Viva lines also are available in bulk. Our extensive QC program ensures the high quality and reproducibility of these silicas. Each lot of material is tested for mean particle size and distribution, pore diameter, surface area, and total metals analysis. You can be confident that you are getting consistent, high quality product. HPLC COLUMNS also available Other stationary phases and particle sizes are available; please inquire. Use these materials for easy scale up to preparative scale chromatography, or for packing your own columns. Bulk Packing Materials Description min. qty. cat.# 5-99 grams grams grams 000 grams 3µm Pinnacle II Bulk Packing Materials Pinnacle II C8 Bulk Packing 5g 9233 /gram /gram /gram /gram Pinnacle II C8 Bulk Packing 5g 9243 /gram /gram /gram /gram Pinnacle II Cyano Bulk Packing 5g 9263 /gram /gram /gram /gram Pinnacle II Phenyl Bulk Packing 5g 9253 /gram /gram /gram /gram 5µm Pinnacle II Bulk Packing Materials Pinnacle II Amino Bulk Packing 5g 9275 /gram /gram /gram /gram Pinnacle II C8 Bulk Packing 5g 9235 /gram /gram /gram /gram Pinnacle II C8 Bulk Packing 5g 9245 /gram /gram /gram /gram Pinnacle II Cyano Bulk Packing 5g 9265 /gram /gram /gram /gram Pinnacle II Phenyl Bulk Packing 5g 9255 /gram /gram /gram /gram 5µm Pinnacle DB Bulk Packing Materials Pinnacle DB C8 Bulk Packing 5g 9445 /gram /gram /gram /gram Pinnacle DB C8 Bulk Packing 5g 9435 /gram /gram /gram /gram Pinnacle DB Cyano Bulk Packing 5g 9465 /gram /gram /gram /gram Description qty. cat.# -9 bottles 0-49 bottles bottles 00 bottles 5µm Ultra Bulk Packing Materials Ultra C Bulk Packing 0g/btl. 905 /btl. /btl. /btl. /btl. Ultra C4 Bulk Packing 0g/btl /btl. /btl. /btl. /btl. Ultra C8 Bulk Packing 0g/btl /btl. /btl. /btl. /btl. Ultra C8 Bulk Packing 0g/btl /btl. /btl. /btl. /btl. Ultra Amino Bulk Packing 0g/btl /btl. /btl. /btl. /btl. Ultra Cyano Bulk Packing 0g/btl /btl. /btl. /btl. /btl. Ultra Phenyl Bulk Packing 0g/btl /btl. /btl. /btl. /btl. new! Silica Bulk Materials Description min. qty. cat.# 5-99 grams grams grams 000 grams Silica Bulk Materials Pinnacle II Silica Bulk Packing, 3µm 5g 9203 /gram /gram /gram inquire/gram Pinnacle II Silica Bulk Packing, 5µm 5g 9205 /gram /gram /gram /gram Pinnacle DB Silica Bulk Packing, 5µm 5g 9405 /gram /gram /gram /gram qty. cat.# -9 bottles 0-49 bottles bottles 00 bottles Ultra Silica Bulk Packing, 5µm 0g/btl /btl. /btl. /btl. /btl. Kromasil Bulk Packings High-purity packing materials in 0 and 6µm. All Kromasil phases available, please inquire. Description min. qty. cat.# grams grams 000 grams Kromasil 00Å Silica, 0µm 200g /gram /gram /gram Kromasil 00Å Silica, 6µm 200g 9200 /gram /gram /gram Kromasil 00Å C8, 0µm 200g /gram /gram /gram Kromasil 00Å C8, 6µm 200g 9203 /gram /gram /gram Kromasil 00Å C8, 0µm 200g /gram /gram /gram Kromasil 00Å C8, 6µm 200g 9204 /gram /gram /gram Kromasil 00Å Chiral DMB, 0µm 200g /gram /gram /gram Kromasil 00Å Chiral DMB, 6µm 200g 9208 /gram /gram /gram Kromasil 00Å Chiral TBB, 0µm 200g 9990 /gram /gram /gram Kromasil 00Å Chiral TBB, 6µm 200g 999 /gram /gram /gram

39 Restek s Exclusive Trident Integral System Convenient and economical leak-free guard cartridge system, extremely easy to install. Versatile configuration protects against all levels of contamination. Integral design eliminates troublesome tubing connections. Trident Integral Guard Cartridge System 37 The system s foundation consists of the analytical column configured with our exclusive Trident end fitting and XF fitting. This configuration contains the standard internal frit as well as a replaceable cap frit, which easily can be changed without disturbing the packed bed. Changing the external frit can reverse the effects of accumulated particles, such as high backpressure or peak distortion. To obtain this basic configuration, simply order any Restek HPLC column, and add the suffix -700 to the catalog number for the column. (Nominal additional charge. ) Lori McGarry Environmental Health & Safety Officer + year of service! For maximum protection against contaminants and particulate matter, the system can be configured with an integral guard cartridge holder (XG-XF), a guard cartridge, and a replaceable external frit. To obtain this configuration, simply order any Restek HPLC column, add the suffix -700 to the catalog number for the column, and order the appropriate XG-XF male fitting (cat.# or 25062, below) and Trident guard cartridges (page 39). Description qty. cat.# price XG-XF Fitting for 0mm Guard Cartridge ea XG-XF Fitting for 20mm Guard Cartridge ea Replacement XF Filter Fitting ea Replacement Cap Frits: 4mm, 2.0µm 5-pk Replacement Cap Frits: 4mm, 0.5µm 5-pk Replacement Cap Frits: 2mm, 2.0µm 5-pk HPLC COLUMNS Trident Integral Guard System Assembled column with Trident integral guard system XF End Fitting Cap Frit XG-XF Fitting Guard Cartridge End Fitting Column with Trident Integral Inlet Fitting (to order add -700 to catalog number of column) and guard cartridge, XG-XF fitting, cap frit, and XF end fitting. Remove the XF end fitting and install the guard cartridge in the end of the column. Add the XG-XF fitting (order cat.#25026 for 0mm guard cartidges, cat.#25062 for 20mm guard cartridges). Re-install the XF end fitting with cap frit. The cap frit can be easily replaced if it becomes contaminated/plugged.

40 38 Trident Direct Guard Cartridge System / Cartridge Holders Trident Direct provides three levels of protection Trident Direct Guard Cartridge System Easy to Use, Low Dead Volume The Ultimate Combination of Convenience and Column Protection Unlike one size fits all guard systems, the Trident Direct system gives you the power to select the right level of protection for your analysis. The system offers three levels of protection and guard cartridges in four dimensions, with a variety of bonded phases to match your analytical column. The economical, leakfree cartridge design provides an unprecedented combination of convenience, economy, and reliability. The foundation of the Trident Direct system is a reusable direct connect holder that easily attaches to any HPLC column using CPI- or Waters-style end fittings.* The system is available in configurations to match different protection level needs: in-line filter, in-line filter with holder for 0mm guard cartridge, and in-line filter with holder for 20mm guard cartridge. The guard cartridges are available in 2. and 4.0mm ID and are interchangeable within the appropriate length holder. HPLC COLUMNS Trident Direct high-pressure filter Protection against particulate matter. Trident Direct 0mm guard cartridge holder with filter Protection against particulate matter and moderate protection against irreversibly adsorbed compounds. *The standard PEEK tip in Trident Direct systems is compatible with Parker, Upchurch, Valco, and other CPI-style fittings. To use Trident Direct systems with Waters-style end fittings, replace the tip with cat.# Description qty. cat.# price High-pressure filter ea mm guard cartridge holder without filter ea mm guard cartridge holder with filter ea mm guard cartridge holder without filter ea mm guard cartridge holder with filter ea Connection tip for Waters-style end fittings ea PEEK tip standard fittings ea Replacement Cap Frits: 4mm, 2.0µm 5-pk Replacement Cap Frits: 4mm, 0.5µm 5-pk Replacement Cap Frits: 2mm, 2.0µm 5-pk Trident Direct 20mm guard cartridge holder with filter Protection against particulate matter and maximum protection against irreversibly adsorbed compounds. Cap frits Trident HPLC In-Line Guard Cartridge Holders A Trident in-line guard cartridge holder can be used with almost any HPLC column by connecting it with a short piece of /6" tubing, appropriate nuts and ferrules, or finger-tight fittings. The system can be used with Restek columns, or with columns from other manufacturers. Holders are available for either 0mm or 20mm guard cartridges. Either size can be purchased with or without a prefilter, which provides added protection against the particles that can shorten the lifetime of the guard cartridge. Description qty. cat.# price Holder for 0mm guard cartridge ea Holder with filter for 0mm guard cartridge ea Holder for 20mm guard cartridge ea Holder with filter for 20mm guard cartridge ea Replacement Cap Frits: 4mm, 2.0µm* 5-pk Replacement Cap Frits: 4mm, 0.5µm 5-pk Replacement Cap Frits: 2mm, 2.0µm* 5-pk *Standard porosity. Holder for 0mm guard cartridge Holder with filter for 0mm guard cartridge Holder for 20mm guard cartridge Holder with filter for 20mm guard cartridge

41 Trident Guard Cartridges 39 Trident HPLC Guard Cartridges 3-pk. 3-pk. 2-pk. 2-pk. Guard Cartridges (0 x 2.mm) (0 x 4.0mm) (20 x 2.mm) (20 x 4.0mm) price Allure AK new! Allure Basix Allure C Allure PFP Propyl Allure Silica Allure Organic Acids Allure Aqueous C Allure Biphenyl phidelity C Pinnacle II Amino Pinnacle II C Pinnacle II C Pinnacle II Cyano Pinnacle II PAH Pinnacle II Phenyl Pinnacle II Biphenyl Pinnacle II Silica Pinnacle DB C Pinnacle DB C Pinnacle DB Cyano Pinnacle DB Phenyl Pinnacle DB Silica Ultra Amino Ultra Aqueous C Ultra C Ultra C Ultra C Ultra C Ultra Carbamate Ultra Cyano Ultra IBD Ultra PFP Ultra Phenyl Ultra Silica Ultra Quat Viva C Viva C Viva C Viva PFP Propyl new! Viva Biphenyl new! Viva Silica Kromasil C Kromasil C Kromasil C Kromasil C Kromasil Amino Kromasil Phenyl Kromasil Silica & 20mm Guard Cartridges HPLC COLUMNS

42 40 Replacement Parts HPLC ACCESSORIES Outlet Ball Valve Needle Seat Assembly new! Lamp, VWD G34A Restek Replacement Parts for Agilent HPLC Systems Description Model # Similar to Agilent part # qty. cat.# price Preventive Maintenance Kit (Includes: rotor seal, needle seat, needle assembly, seat cap) kit Autosampler Preventive Maintenance Kit (Includes: rotor seal, needle assembly, needle seat) 00 G kit 2527 Pump Maintenance Kit (Includes: PTFE frit, outlet cap, active inlet cartridge, gold disk seal, 2 piston seals, glass solvent filter) 050 & 00 G kit Outlet Ball Valve, Binary Pump 00 G ea Outlet Ball Valve 050 & 00 G ea Sieves for Outlet Valve 050 & pk Check Valve Cartridge Assembly ea Piston Seals, Teflon w/graphite 050 & pk Piston Seals, Teflon w/graphite 050 & pk Piston Seals (Black) pk Seal Wash Kit, Binary Pump (4 seals, 4 gaskets) 00 kit Seal Wash Kit (2 seals, 2 gaskets) 00 kit Wash Seal 050 & ea Sapphire Piston 050 & ea Sapphire Piston ea Needle Seat ea Needle Seat ea Needle Seat Assembly 00 G ea Needle Assembly 00 G ea Rotor Seal (not for use with 725 injection valve) ea Rotor Seal ea Rotor Seal (Rheodyne -style) ea Frits, PTFE 050 & pk Seal, Gold Disk (outlet) 050 & ea Outlet Cap 050 & pk Outlet Cap & Gold Seal Assembly 050 & 00 2-pk Connecting Tube 050 & 00 G ea Bottle Head Assembly G ea Detector Lamp, 090 DA, 050 VW/DA/MWD 090, ea Lamp, DAD G35A, G365A ea Lamp, VWD G34A 00 G ea Deuterium Lamp ea G32 Fluorescence Detector Flash Lamp ea Lamp, DAD Long Life Deuterium (2000 hours) ea new! Capillary SS Tubing with fittings Mixing Capillary Assembly Capillary Stainless Steel Tubing Assemblies for Agilent HPLC Systems Description Model # Similar to Agilent part # qty. cat.# price Capillary SS Tubing, 30mm x 0.7mm ID, with fittings ea Capillary SS Tubing, 800mm x 0.7mm ID, with fittings ea Capillary SS Tubing, 80mm x 0.7mm ID, with fittings 00 G ea Capillary SS Tubing, 700mm x 0.25mm ID, with fittings ea Capillary SS Tubing, 700mm x 0.25mm ID, with fittings ea Seat Capillary, SS Tubing, 0.7mm ID ea Capillary SS Tubing, 05mm x 0.7mmID ea Mixing Capillary Assembly 00 G ea Capillary SS Tubing, Valve to Metering Head 00 G ea Capillary SS Tubing, 50mm x 0.7mm ID ea Capillary SS Tubing, 280mm x 0.7mm ID ea Capillary SS Tubing, 400mm x 0.7mm ID ea /6" SS Fitting, Front and Back Ferrules pk

43 Outlet Cap and Gold Seal Assembly Tool for Agilent 050 & 00 HPLC Systems Easily install the gold seal into the outlet cap. Replacement Parts 4 new! Put Outlet Cap on male part of Assembly Tool. Push the Assembly Tool together. Hold onto Outlet Cap and pull Assembly Tool apart. Put Gold Seal over pin on male part of Assembly Tool. Restek Replacement Parts for Beckman HPLC Systems Push the Assembly Tool together and press the Gold Seal in the Outlet Cap. Pull the Assembly Tool apart and remove assembled Outlet Cap and Gold Seal. Description qty. cat.# price Outlet Cap and Gold Seal Assembly Tool for Agilent 050 & 00 HPLC Systems ea Description Model # Similar to Beckman part # qty. cat.# price Inlet Check Valve Assembly 0A&B, 2, 4M, 6, 8, 25, 26, 27, 28 Pumps ea Outlet Check Valve Assembly 0A&B, 2, 4M, 6, 8, 25, 26, 27, 28 Pumps ea Inlet Check Valve Cartridge for Check Valve Assembly 0A&B, 2, 4M, 6, 8, 25, 26, 27, 28 Pumps ea Outlet Check Valve Cartridge for Check Valve Assembly 0A&B, 2, 4M, 6, 8, 25, 26, 27, 28 Pumps ea Guide Sleeve for Graphite Plunger Guide 0 Series Pumps ea Outlet Check Filter Frit Assembly 0 Series Pumps ea Piston Guide Assembly 0 Series and 2 Pumps ea Plunger 0 Series and 2 Pumps ea Plunger Seal 0 Series Pumps ea Plunger Seal 2 Pumps ea Sapphire Plunger 4M, 6, 8, 25, 26, 27, 28 Pumps ea Plunger Seal 4M, 6, 8, 26, 27, 28 Pumps ea Pump Seal, Gold* 4, 6, 25, 26, 27, 28 Pumps ea Plunger Wash Seal 25, 26, 27, 28 Pumps ea Deuterium Lamp DU60, 62, 64, ea *Ultra-high molecular weight polyethylene (UHMWPE). Outlet Check Valve Assembly Sapphire Plunger HPLC ACCESSORIES Restek Replacement Parts for Hitachi HPLC Systems Description Model # Similar to Hitachi part # qty. cat.# price SS Check Valve Cartridges ( /6" Ruby Ball) 655/6000/6200 AN pk Inlet Check Valve Assembly 655/6000/6200 AN ea Outlet Check Valve Assembly 655/6000/6200 AN ea Plunger Assembly 655, 6000, 6200, ea Plunger Seal L655A, 6000, 6200, ea Plunger Seal, Gold 655, 6000, 6200, ea Inlet Check Valve Assembly L-700 AN ea Outlet Check Valve Assembly L-700 AN ea Rotor Assembly for Dilutor Valve AS-7200 Autosampler ea Rotor Seal Kit AS-7200, AS-7250 Injection Valve AN0-088 ea Deuterium Lamp, Prealigned L4000, L4200, L4250, L ea

44 42 Replacement Parts HPLC ACCESSORIES new! Restek Replacement Parts for PerkinElmer HPLC Systems Description Model # Similar to PerkinElmer part # qty. cat.# price Inlet Check Valve PE 200, 4, 250, 400, 40, ea Outlet Check Valve PE 200, 4, 250, 400, 40, ea High Pressure Seal Kit PE Series 200 Pumps--, 2, 3, 3B, 4, 0, 250, 400, 40, 620, Int Pump ea Standard High Pressure Seal PE Series 200 Pumps--, 2, 3, 3B, 4, 0, 250, 400, 40, 620, Int Pump ea Low Pressure Seal PE Series 200 Pumps ea Needle PE Series 200 Pumps N ea Deuterium Lamp PE Lambda--2, 5, 7, 8, 0,, 2, 4, 5, 6, 7, 8, 9, 20, 40, 800, 900 B06097 ea Deuterium Lamp PE 200/785A N ea Restek Replacement Parts for Shimadzu HPLC Systems Description Model # Similar to Shimadzu part # qty. cat.# price Inlet Check Valve LC-6A, LC-0AS ea Inlet Check Valve LC-600, LC-9A, LC-0AD ea Inlet Check Valve LC-0ADvp ea Inlet Check Valve LC-0AT, LC-0ATvp ea Outlet Check Valve LC-6A, LC-0AS ea Outlet Check Valve Rebuild Kit LC-6A, LC-0AS pk Outlet Check Valve LC-600, LC-9A, LC-0AD, LC-0AT ea Outlet Check Valve LC-0ADvp, LC-0ATvp ea Plunger Seal LC-6A ea Plunger Seal, Polyethylene LC-0AS, LC-0AT, LC-0ATvp ea Plunger Seal LC-600, LC-9A, LC-0AD ea Plunger Seal LC-0ADvp ea Plunger Seal, Gold LC-0ADvp ea Plunger Seal SIL-0ADvp, LC-0ATvp ea Plunger Rinse Seal LC-0AS ea Sapphire Plunger LC-6A ea Sapphire Plunger LC-0AS ea Sapphire Plunger LC-600, LC-9A, LC-0AD ea Needle Seal SIL-0A, 0XL, 0ADvp ea Rotor Seal SIL-0ADvp ea Rotor Seal Assembly SIL-0A, 0AXL ea Stator Assembly SIL-0A, 0AXL ea Syringe, 500µL SIL-0A, 0AXL ea Plunger Assembly, Ceramic LC-0ADvp ea Plunger Assembly, Ceramic LC-0ATvp ea Plunger Assembly, Sapphire LC-0ADvp ea Deuterium Lamp SPD-6A ea Deuterium Lamp SPD-0A, 0AV, M20A ea Julie Kowalski Innovations Chemist 2+ years of service!

45 Replacement Parts 43 Restek Replacement Parts for Thermo Separation HPLC Systems Description Model # Similar to SP/TSP part # qty. cat.# price Inlet Check Valve Assembly SP8800 & P-Series Pumps A ea Outlet Check Valve Assembly SP8800 Series Pumps A ea Piston SP8800 & P-Series Pumps A ea Back-up Seal SP8800 & P-Series Pumps A ea Plunger Seal, Gold Superseal SP8800 & P-Series Pumps A ea Check Valve and Transducer Assembly P-Series Pumps A ea Kel-F Washer P-Series Pumps A ea TSP AS00, 300, 000, 3000, 3500, Rotor Seal Assembly, Rheodyne , and 8880 Autosamplers ea TSP AS00, 300, 000, 3000, 3500, Syringe Assembly, 250µL 8875, and 8880 Autosamplers A ea Syringe, 500µL 8800 Series ea Lamp, UV Linear UV-200, 203, 204, 205, 206, and UV 00, 50, 000, and 2000 Detectors ea Description Model # Similar to TSP part # qty. cat.# price Check Valve Cartridge LDC Constametric Pumps ea Sapphire Plunger LDC Constametric Pumps ea Plunger Seal Kit, Gold LDC Constametric Pumps ea Plunger Seal, Black LDC Constametric Pumps ea Plunger Seal, Gold LDC Constametric Pumps ea Lamp, Deuterium LDC SM-I, II, III, 3000, 300, 300X, and 4000 Detectors ea Lamp, Deuterium Pre-aligned LDC 3200 and 400 Detectors ea Lamp, UV Piston Syringe Assembly, 250μL HPLC ACCESSORIES Restek Rheodyne Style Replacement Parts for Waters HPLC Systems Description Similar to Rheodyne part # qty. cat.# price 700 Vespel Rotor Seal ea Vespel Rotor Seal ea Isolation Seal, ea For easy access to Rheodyne fittings, use a ValvTool Wrench see page 54. Restek Replacement Parts for Waters Detectors Similar to Description Model # Waters part # qty. cat.# price Window Gasket 484, 486, ea Lamp Side Gasket 484, 486, ea Quartz Lens ea Xenon Lamp (w/o holder or mirror) 470 ea Xenon Lamp 474 ea Deuterium Lamp (UV/Vis) 480, ea Deuterium Lamp (UV/Vis) ea Deuterium Lamp (UV/Vis) ea Deuterium Lamp 996, 2996 WAT ea Deuterium Lamp 2487 WAS0842 ea Deuterium Lamp, long life (2000 hours)* ea *Standard lamps have nominal 000 hour life. also available HPLC autosampler syringes. See pages

46 44 Replacement Parts HPLC ACCESSORIES Preventative Maintenance Kit for 77 Autosampler Inlet Check Valve Assembly new! 77 Seal Pack (Rebuilt) with Needle new! Restek Replacement Parts for Waters HPLC Systems Description Model # Similar to Waters part # qty. cat.# price Preventative Maintenance Kit Includes: sparge diffuser, filter insert, compression screws, SS ferrule, battery for 2690/77, 250µL WISP syringe, seal wash plunger seal kits (2), wash tube seal kits (4), 2690 seal pack rebuild kit (steel bodies not included), 2690 head plunger seal kits (2), solvent reservoir 20µm filters (4), Alliance check valve cartridges (2), Alliance plunger assemblies (2), 2690 face seals (4) Alliance 2690, 2695 WAT kit 2543 Preventative Maintenance Kit Includes: PerformancePLUS cartridges (2), sparge diffusers (4), Super Seals (2), solvent reservoir 20µm filters (2), sapphire plungers (2), reference valve rebuild kit, inlet manifold kit 600 Pump WAT kit 2544 Preventative Maintenance Kit Includes: 77 seal pack with needle, filter insert, 250µL WISP syringe 77 Autosampler WAT kit 2545 Preventative Maintenance Kit Includes: 66/326/625 plunger assemblies (2), pump seal kit, sparge diffusers (4), solvent reservoir 20µm filters (4), 66 cartridge assemblies (4) 66 Pump WAT kit 2546 Preventative Maintenance Kit Includes: PerformancePLUS check valves (2), sparge diffuser, solvent 0µm filter, sapphire plungers (2), plunger seals (2) 55 Pump WAT kit 2659 Inlet Check Valve Assembly M6KA, 50, 50, 55, 590, 600E 33679, 2524 ea Inlet Check Valve Housing M6KA, 50, 50, 55, 590, 600E ea Inlet Check Valve Rebuild Kit M6KA, 50, 50, 55, 590, 600E pk Outlet Check Valve Assembly (Actuator Style) M6KA, 50, 50, 55, 590, 600E ea Outlet Check Valve Housing (Actuator Style) M6KA, 50, 50, 55, 590, 600E 2522 ea Outlet Check Valve Rebuild Kit (Actuator Style) M6KA, 50, 50, 55, 590, 600E pk Outlet Check Valve Assembly (Ball & Seat Style) M6KA, 50, 50, 55, 590, 600E 2526 ea Outlet Check Valve Housing (Ball & Seat Style) M6KA, 50, 50, 55, 590, 600E ea Outlet Check Valve Rebuild Kit (Ball & Seat Style) M6KA, 50, 50, 55, 590, 600E pk Inlet Check Valve Assembly, 225µL (Extended Flow) M6KA, 50, 50, 55, 590, 600E ea PerformancePLUS Check Valve Cartridge M6KA, 50, 50, 55, 590, 600E pk Check Valve Rebuild Kit (Extended Flow) M6KA, 50, 50, 55, 590, 600E pk PerformancePLUS Check Valve Housing M6KA, 50, 50, 55, 590, 600E ea Check Valve Cartridges Alliance WAT pk Super Seal for Analytical Heads M6KA, 50, 50, 55, 590, 600E 22946, ea Plunger Seal, Gold for Analytical Heads* M6KA, 50, 50, 55, 590, 600E ea Plunger Seal, Black for graphite-filled Teflon M6KA, 50, 50, 55, 590, 600E 2663 ea Plunger Seal, Black for EF Heads 50, 590, 600E ea Plunger Seal, Gold for EF Heads 50, 590, 600E ea Seal Wash Plunger Seal Alliance WAT pk Head Plunger Seal Kit Alliance WAT pk Head Plunger Seal Kit (Black) Alliance WAT pk Insert Seal Parts Kit M6KA, 50, 50, 55, 590, 600E 6002 kit Sapphire Plunger M6KA, 50, 590, ea Sapphire Plunger (Extended Flow) 50, 590, 600E ea Sapphire Plunger M45, M ea Sapphire Plunger Assembly Kit M55 WAS ea Sapphire Plunger 66, 625, ea Sapphire Plunger Alliance WAT ea Pressure Transducer M6KA, M45, 50, 50, 590, 600E WAT ea Draw-Off Tube Assembly 50, 55, 600, ea Inlet Manifold Kit M45, 50, 50, 590, 600E kit 2542 Ferrule, Stainless Steel ea Wash Face Seal Alliance 2690 WAT2707 ea Wash Tube Seal Kit Alliance 2690 WAT pk Rebuild Kit, Reference Valve 600 Pump ea Compression Screws, Stainless Steel Alliance pk Battery Alliance 2690, 77, 486, 484 ea Seal Pack Rebuild Kit, without Seal Wash Tube Assembly Alliance 2690 WAT2709 ea Seal Pack (Rebuilt) with Needle 77 Autosampler 4559 ea Seal Kit, Pump 66 Pump, 626, ea Cartridge Assembly, Stainless Steel 66 Pump ea *Ultra-high molecular weight polyethylene (UHMWPE).

47 Valves 45 ASI Piston Seals for Agilent HPLC Systems Over time the piston seal in every HPLC system becomes worn and must be replaced. Our piston seals are constructed of Teflon material with an additive that reduces their tendency to cold flow and improves their wear characteristics. Teflon seals are more resistant to any HPLC solvent than UHMWPE seals, but they are less wear resistant and rugged. For Agilent 050 and 00 HPLC systems. Description qty. cat.# price ASI Piston Seal ea ASI Piston Seal 0-pk ASI Check Valves ASI subjects each valve to a rigorous series of tests, including self-priming capability, which means you typically won t need a syringe or draw-off valves to prime your pump. A high-pressure test ensures that the valve will not leak, even at pressures up to 2,000psi (82,737kPa). A low-pressure test verifies that the valve closes properly, even without a high backpressure force. The cartridge design allows easy, convenient replacement of worn or damaged valves. ASI Check Valves for Agilent 050 & 00 Description Material qty. cat.# price Outlet Check Valve (cartridge and housing) Sapphire & Ruby Ball & Seat ea Outlet Check Valve (cartridge and housing) Ceramic Ball & Seat ea Outlet Cartridges Sapphire & Ruby Ball & Seat ea. 257 Outlet Cartridges Ceramic Ball & Seat ea Outlet Housing ea ASI Check Valves for Waters Alliance Model 2690 Description Material qty. cat.# price Inlet and Outlet Cartridges Sapphire & Ruby Ball & Seat ea Inlet and Outlet Cartridges Ceramic Ball & Seat ea Inlet and Outlet Check Valves for Waters Instruments HPLC ACCESSORIES Valves for HPLC Our valves have low internal volumes and are rated to 5,000psi (03,42kPa). They feature a 2-piece stem assembly in which the rotating upper shank is coupled through a ball joint to a static lower stem. Only fingertight torque is required to make the seal. new! Description qty. cat.# price Two-Way Through Valve for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea Two-Way Angle Valve for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea Three-Way Bottom Vent Valve for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea Side Vent Valve for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea Dual Stem Three Way Valve for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea Prime/Purge Valve for HPLC, /4-28 Flanged Seat, Stainless Steel, with Tubing and Fittings ea Prime/Purge Valve Repair Kit for Prime/Purge Valve kit Side Vent Valve In-Line Check Valves for HPLC Our 36 stainless steel in-line check valves have a soft seat and /6" fittings. Nuts and ferrules are included. Description qty. cat.# price Check Valve, Soft Seat, for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea new!

48 46 Filters High-Pressure Frit-Type In-Line Filter Restek s high-pressure in-line filter is a stand-alone version of the Trident column protection system, specifically designed for ease of use, low dead-volume, and flexibility. The filter has a replaceable, PEEK encapsulated 36 stainless steel frit with a surface area of 2mm². The standard frit with 2.0µm porosity may be replaced with an optional 0.5µm porosity frit. Use of this filter can greatly extend column life, thereby reducing costs and saving maintenance time. Tubing OD /6"; connectors CPI. Description Porosity qty. cat.# price Frit-Type In-Line Filter 2.0µm ea Replacement Cap Frits: 4mm 0.5µm 5-pk Replacement Cap Frits: 4mm 2.0µm 5-pk HPLC ACCESSORIES High-Pressure Cup-Type In-Line Filter This high-pressure cup-type filter can be used in fluid streams operating to 5,000psi (03,42kPa). The cup-shaped filter elements have a large (2.5 cm 2 ) surface area to give long operating lifetime. Mounted in screw-type adapters, they are easily removed for cleaning. Normally, backflushing and cleaning in an ultrasonic bath with an appropriate solvent will restore them. If they become permanently clogged, replacement elements are available. Housings and all wetted parts are 36 stainless steel. Filters are packaged with gland nuts and ferrules. A bulkhead type fitting is available for thru-panel mounting. Tubing OD /6"; connectors CPI. Description Porosity qty. cat.# price Cup-Type In-Line Filter 0.5µm ea Cup-Type In-Line Filter 2.0µm ea Replacement Filter Elements & Seals 0.5µm 2-pk Replacement Filter Elements & Seals 2.0µm 2-pk Low-Pressure Slip-On Inlet Filter for Mobile Phase Reservoir A type 36 stainless steel tip with a Tefzel collar seals to a corrosion-resistant type 36 stainless steel filter element. The slip-on filter easily attaches to the pump inlet line, without the use of wrenches. The universal tip accommodates standard Teflon tubing inner diameters. The cylindrical filter is standard 0µm porosity. /8" OD. Fits Altex, ISCO, LDC, Varian, Waters, PerkinElmer, and other pumps. Description qty. cat.# price Slip-on Inlet Filter ea Low-Pressure CPI Inlet Filter for Mobile Phase Reservoir A 36 stainless steel knurled cap and Tefzel CPI ferrule seal to /8" OD Teflon tubing when finger-tightened onto the precision-machined filter holder. The filter element is replaceable. Standard 0µm porosity protects delicate pump components from particles but introduces very little pressure drop. /8"OD.May be used as a helium sparging diffuser. Description qty. cat.# price CPI Inlet Filter ea Replacement Elements: 0µm filter 2-pk also available Trident Direct high-pressure filter protection against particulate matter. See page 38 for details.

49 Filters 47 Mobile Phase Sparge Filter; Inlet Filter The 2µm helium sparge filter is an inexpensive way to prepare and maintain mobile phases free of dissolved gas. Both filters are made from 36 stainless steel and PEEK and are compatible with most solvents. Description qty. cat.# price Mobile Phase Sparge Filter: 2µm ea. 253 Inlet Filter: 0µm ea Inlet Filter: 20µm ea Last Drop Filter The flat filter element sits parallel to the bottom of the mobile phase reservoir, allowing the filter to draw 98% of the mobile phase without drawing air into the system. Conventional cylindrical mobile phase filters begin to draw air into the system when approximately 0% of the solvent remains in the reservoir. The Last Drop Filter allows more analyses per batch of mobile phase and helps reduce hazardous waste. 22.mm OD. Description qty. cat.# price Last Drop Filter: 2µm ea Last Drop Filter: 0µm ea HPLC ACCESSORIES Kontes Membrane Microfiltration Glassware 47mm filtration apparatus with fritted glass support is recommended for routine filtration of corrosive liquids and removal of particles from HPLC solvents. The ground joint connection eliminates phthalate contamination that can occur when using silicone or neoprene stoppers. The support base has a coarse porosity glass frit and an integral vacuum connection, located above the drip tip to prevent contamination of the vacuum line with filtrate droplets. Each apparatus includes a funnel, an anodized aluminum clamp, a 47mm fritted glass support base, and a filtration flask. Kontes All-Glass Microfiltration Apparatus qty. cat.# price 300mL Funnel, 000mL Flask ea. KT mL Funnel, 2000mL Flask ea. KT mL Funnel, 4000mL Flask ea. KT Replacement Parts for Kontes Microfiltration Apparatus qty. cat.# price 40/35 PTFE Joint Sleeve 6-pk. KT Flask Cap, 40/35 Outer Joint ea. KT Fritted Glass Support, 47mm, 40/35 Joint ea. KT Glass Funnel, 47mm, 00mL ea. KT Glass Funnel, 47mm, 300mL ea. KT Glass Funnel, 47mm, 500mL ea. KT Glass Funnel, 47mm, 000mL ea. KT Flask, 000mL, 40/35 Joint ea. KT Flask, 2000mL, 40/35 Joint ea. KT Flask, 4000mL, 40/35 Joint ea. KT Aluminum Clamp, 47mm ea. KT Membrane Filters qty. cat.# price Polyproylene Membrane Filters, 47mm, 0.45µm 00-pk Polyproylene Membrane Filters, 47mm, 0.22µm 00-pk Nylon Membrane Filters, 47mm, 0.45µm 00-pk Nylon Membrane Filters, 47mm, 0.22µm 00-pk new! tech tip Mobile Phase Additives Mobile phase additives such as triethylamine, trifluoroacetic acid, and ion-pairing reagents can compete with sample ions, decreasing sensitivity and, in some cases, reducing sample ion intake into the MS. To obtain symmetric peaks and/or sufficient retention, use base deactivated, state-of-the-art Type B silica packings that minimize the need for additives.

50 48 Mobile Phase Accessories HPLC ACCESSORIES Degasys Ultimate Degasser provides highly stable baselines Ultimate Degasser Off LC_096 Ultimate Degasser On Mobile Phase: water:methanol 50:50.0 ml/min. 20nm Mobile Phase Degasser Dissolved oxygen can cause flow rate instability and increased baseline noise. Also, it has a quenching effect on fluorescence detection and increases the background of UV detectors. Dissolved gases can out-gas in the HPLC system, forming bubbles in check valves, at connections, or in detector flow cells. In-line vacuum degassing is more effective at removing dissolved gas from mobile phases than sonication or helium sparging. In-line degassers work by withdrawing gas across a gas-permeable membrane encased in a sealed chamber. Traditionally, the membrane has been made of PTFE tubing, but the Degasys Ultimate Degasser uses tubing composed of an amorphous fluoropolymer that is 200 to 300 times more gas permeable than PTFE. This translates into the ability to use shorter tubing for removing dissolved gas. This new material also has better tubular burst strength than PTFE. To prevent cross contamination, each channel on this Degasys unit is individually encased within its own vacuum chamber. Specifications: Residual Oxygen Pressure Loss Internal Volume Wetted Parts Max Flow Rate 0.9ppm 0.24psi (.65kPa) 500µL Teflon AF, PTFE, ETFE, PPS 7mL/min./channel At a flow rate of ml/min. Description qty. cat.# price 0V Mobile Phase Degasser (4 Channel, 7mL/min./channel) ea V Mobile Phase Degasser (4 Channel, 7mL/min./channel) ea Do not use the Degasys system with solutions containing TFA at concentrations greater than 5%. Solvent Debubbler Bubbles in an HPLC system can cause check valve malfunctions and pump cavitation, seriously affecting pump performance. The debubbler removes bubbles from the fluid stream before it enters the pump. Special geometry at the base of the housing allows bubbles entrained in the inlet fluid stream to rise and be trapped in the reservoir. The gas/liquid interface is easily visible through the translucent wall of the device. Loosening the airtight cap releases the trapped gas. The debubbler is fitted with a bracket and universal connecting tips. Description qty. cat.# price Solvent Debubbler with Bracket ea Sonic Debubbler Fast. Easy to use. Less solvent waste; less clean-up. Just touch the Sonic Debubbler to the inlet line or check valve sonic vibrations will quickly dislodge or redissolve trapped air bubbles. Reduces downtime or conversion time from one mobile phase to another. Description qty. cat.# price Sonic Debubbler (0V) ea Sonic Debubbler (220V) ea Terry Reid Technical Service Representative 8+ years of service!

51 Bottle Tops 49 Opti-Cap Bottle Top The most economical way to helium-sparge and deliver HPLC mobile phases. The Opti-Cap top fits all standard GL45 bottles and has two /8-inch holes and one /6-inch hole for tubing. Description qty. cat.# price Opti-Cap (Cap and PEEK Plug) ea Opti-Cap Kit (Opti-Cap, 3 meters of tubing, sparging filters) kit 2530 Opti-Cap Kit with L Bottle kit Opti-Cap Kit with 2L Bottle kit Related items and replacement parts qty. cat.# price SS Mobile Phase Mobile Phase Sparge Filter: 2µm ea. 253 Mobile Phase Inlet Filter: 0µm ea Teflon Tubing, /8" OD x 0.094" ID x 3m (2.4mm ID) 3m Teflon Tubing, /8" OD x 0.063" ID x 3m (.6mm ID) 3m PEEK Plug, /4"-28 threads 3-pk L Graduated Safety-Coated Bottle GL-45 threads ea L Graduated Safety-Coated Bottle GL-45 threads ea Eco-Cap Bottle Top, PTFE Fits all standard GL-45 bottles; has two /8-inch holes and one /6-inch hole for tubing. Description qty. cat.# price Eco-Cap Bottle Top, includes Male Luer Plug ea Opti-Cap Kit with bottle HPLC ACCESSORIES Hub-Cap 4 Liter Bottle Tops Most bottles use a GL45 cap. New Hub-Cap bottle tops are a great way to neatly keep your mobile phase lines where they belong. Use instead of parafilm, aluminum foil, or tape on your mobile phase reservoirs. new! Hub-Cap (assembly of the bottle cap and plug) Description qty. cat.# price Hub-Cap (assembly of the bottle cap and plug) kit 2654 Hub-Cap Multi-pack 3-pk Hub-Cap Adapters Allow the use of the Opti-Cap with 4-liter solvent bottles. new! Description qty. cat.# price Hub-Cap Adapter ea Hub-Cap Adapter Multi-pack 3-pk Hub-Cap Adapter and Opti-Cap kit Hub-Cap Filter Kit Kit includes: bottle adapter, bottle adapter nut, filter inlet cap, grid support, vacuum hose barb, tube compression fitting, 47mm grid, 47mm.22µm filter membrane, 47mm.45µm filter membrane, /4" OD x /8" ID ultra chemical resistant, Teflon FEP lined Tygon tubing (3'), 6" x 6" box with shrink wrap insert. Description qty. cat.# price Hub-Cap Filter Kit kit Replacement Membrane Filters qty. cat.# price Polyproylene Membrane Filters, 47mm, 0.45µm 00-pk Polyproylene Membrane Filters, 47mm, 0.22µm 00-pk Nylon Membrane Filters, 47mm, 0.45µm 00-pk Nylon Membrane Filters, 47mm, 0.22µm 00-pk Hub-Cap Adapter new! Hub-Cap Adapter and Opti- Cap

52 50 Column Heater and Heater/Cooler, Gas Trapping System new! Sidewinder Column Heater Easy to set up! Operation range: 5 C above ambient to 85 C, ± C. Lightweight, compact design fits in small spaces. Column holder can be placed in any orientation. HPLC ACCESSORIES Sidewinder temperature control module new! This unique design completely encloses any HPLC analytical column up to 25cm in length. Two lengths of heater jackets are available: the short column holder accommodates columns up to 0cm in length, while the long column holder holds columns up to 25cm in length. The control module provides optimum heating performance, accuracy to within C, and stability to within 0. C. The new Sidewinder controller has fast 0Hz sampling for improved responsiveness. Power requirements: 24V control unit for maximum stability; RS232 control allows external programming. Description qty. cat.# price Temperature Control Module and Long Column Holder, 25cm Holder ea Temperature Control Module and Short Column Holder, 0cm Holder ea Sidewinder Heater/Cooler Temperature Control Module Operation range: 5-55 C, ±0.2 C. Ability to program multiple temperature points. Accommodates columns up to 30cm in length and 7.8mm ID. Compact design. The Sidewinder heater/cooler unit has a doubly insulated cover to maintain the programmed temperature to within 0.2 C. The 24V control unit provides maximum stability and rapid equilibration times; RS232 control allows external programming. Description qty. cat.# price Sidewinder Heater/Cooler Temperature Control Module ea All Sidewinder temperature control products carry the value recognized CE mark. Each unit meets the demanding electromagnetic emission standards of the new European Union Directives, United States standards, and Canadian standards. Mobile Phase Pre-heater Heats mobile phase before it enters a heated column. Minimizes temperature changes, to help keep analyte peaks sharp. Description qty. cat.# price Mobile Phase Pre-heater ea it s a fact A Super-Clean quick-change cartridge system efficiently removes hydrocarbons from nitrogen! Charcoal Filters Super-Clean Gas Trapping System for LC/MS Changing filters is quick and easy. Up to 20L of hydrocarbon-free nitrogen per minute. 2-Position Base Plate The Super-Clean Gas Trapping System is the latest technology in cartridge-style gas filtration for purifying nitrogen, and is ideal for use in LC/MS systems. The two-position baseplate (installed in the gas line) allows cartridges to be exchanged without introducing oxygen into the system. Spring-loaded check valves seal when a cartridge is removed and open only when a new cartridge has been locked in place. There is no need for loosening and tightening fittings every time you change cartridges, and your system cannot become contaminated during the changing process. To meet the high flow needs of the LC/MS system, the charcoal-filled cartridges are positioned and connected in parallel. The incoming gas stream is split equally between the cartridges, and the two streams are rejoined after purification but before the gas exits the baseplate. This approach allows longer contact between the nitrogen and the adsorbent, ensuring higher gas purity and eliminating a potential source of contaminants to your analyses. A handy date wheel, included with the system, indicates the cartridge installation date and the recommended replacement date. Description qty. cat.# price Super-Clean Gas-Trapping System (2-position baseplate, 2 charcoal filters) ea Position Baseplate ( /4" Fittings) ea Replacement Hydrocarbon (Charcoal) Filters 2-pk. 2206

53 Survival Kits 5 PEEK Survival Kit for HPLC The PEEK Survival Kit is an invaluable parts kit that contains tubing, fittings, and tools essential for setting up and maintaining your HPLC system: PEEK tubing, connectors, and elbows, Teflon tubing, a tubing cutter and extra blades, a ValvTool wrench, open-end wrenches, and more. For start-up and maintenance in all HPLC systems. Description qty. cat.# price PEEK Survival Kit for HPLC kit Contents: Description qty. cat.# price PEEK Column Connector (beige, round body) 0-pk PEEK Tubing, /6" OD x 0.005" ID x 3m (red stripe) ea PEEK Tubing, /6" OD x 0.007" ID x 3m (yellow stripe) ea PEEK Tubing, /6" OD x 0.00" ID x 3m (blue stripe) ea PEEK Tubing Elbow 90 5-pk PEEK Tubing Elbow 80 5-pk Teflon Tubing, /8" OD x 0.063" ID x 3m (.6mm ID) 3m Teflon Tubing, /8" OD x 0.094" ID x 3m (2.4mm ID) 3m Tubing Clip 5-pk ValvTool Wrench ea Open-End Wrenches ( /4" x 5 /6") 2-pk.* 200 Clean-Cut Tubing Cutter ea Replacement Blade for Clean-Cut Cutter ea PEEK Union Connector /6" 2-pk Mobile Phase Sparge Filter: 2µm ea. 253 Inlet Filter: 0µm ea *Kit contains wrench, replacement (cat.# 200) is 2-pk. PEEK Survival Kit HPLC ACCESSORIES Stainless Steel Survival Kit for HPLC The stainless steel survival kit contains a wide range of tubing, fittings, and tools necessary to set up and maintain your HPLC system: a selection of lengths and IDs of /6-inch tubing, plus nuts, ferrules, a ValvTool wrench, and a zero-dead-volume union. Description qty. cat.# price Stainless Steel Survival Kit for HPLC kit Contents: Description color qty. cat.# price HPLC Capillary Tubing, SS /6" x 0.005" x 5cm red 3-pk HPLC Capillary Tubing, SS, /6" x 0.005" x 0cm red 3-pk HPLC Capillary Tubing, SS, /6" x 0.005" x 20cm red 3-pk HPLC Capillary Tubing, SS, /6" x 0.005" x 30cm red 3-pk HPLC Capillary Tubing, SS, /6" x 0.007" x 5cm gray 3-pk HPLC Capillary Tubing, SS, /6" x 0.007" x 0cm gray 3-pk HPLC Capillary Tubing, SS, /6" x 0.007" x 20cm gray 3-pk HPLC Capillary Tubing, SS, /6" x 0.007" x 30cm gray 3-pk HPLC Capillary Tubing, SS, /6" x 0.00" x 5cm blue 3-pk HPLC Capillary Tubing, SS, /6" x 0.00" x 0cm blue 3-pk HPLC Capillary Tubing, SS, /6" x 0.00" x 20cm blue 3-pk HPLC Capillary Tubing, SS, /6" x 0.00" x 30cm blue 3-pk HPLC Capillary Tubing, SS, /6" x 0.020" x 5cm yellow 3-pk HPLC Capillary Tubing, SS, /6" x 0.020" x 0cm yellow 3-pk HPLC Capillary Tubing, SS, /6" x 0.020" x 20cm yellow 3-pk HPLC Capillary Tubing, SS, /6" x 0.020" x 30cm yellow 3-pk /6" Rheodyne Style Nut 0-pk /6" Rheodyne Style Ferrule 0-pk ValvTool Wrench ea /6" Stainless Steel Ferrules 0-pk /6" Stainless Steel Nuts 0-pk Zero-Dead-Volume Internal Union ea Stainless Steel Survival Kit it s a fact Survival Kits are convenient and economical, compared to ordering individual items!

54 52 Fittings, Connectors, Tools PEEK Fitting Extractor Drill into the broken fitting, then screw the extractor into the fitting and remove it easily. Eliminates the need for heat or other techniques that could damage your column. HPLC ACCESSORIES Description qty. cat.# price PEEK Fitting Extractor ea PEEK Union Connector Allows you to quickly and reliably connect two pieces of /6-inch tubing. 0.3mm union bore. End fittings included. Description qty. cat.# price PEEK Union Connector /6" 2-pk Zero-Dead-Volume Internal Union Ends of tubing seat squarely at bottoms of fitting details. 300 series stainless steel. For /6-inch OD tubing. Stainless steel ferrules included. Description Union Bore Valco # qty. cat.# price Internal Union 0.5mm ZUXC ea Internal Union 0.25mm ZUC ea Internal Union 0.75mm ZU ea Internal Union /6" ZUT ea Figure Incompatible fittings create problems. Tubing and ferrule seated correctly Improving Column Connections A good connection is necessary for trouble-free chromatography. Connecting incompatible fittings from different manufacturers can result in leaks, poor peak shape, and increased void volume (Figure ). Each type of end-fitting has a unique seat depth or style. Generally, Restek, Valco, Parker, and Upchurch Scientific fittings are interchangeable; whereas Waters, SSI, Rheodyne, and Gasukura fittings are not (Figure 2). Our wrenchless universal 0-32 PEEK column connector (cat.# 2505, page 54) can be used with any style of end fitting, and all /6-inch tubing. It is reusable and will adjust to any seat, depth, or style. Figure 2 Fitting styles differ among various manufacturers. Tubing not seated, causing dead volume Parker Valco Waters Swagelok Parker A-Lok Ferrule can t seal Rheodyne

55 HPLC Piston Seal Insertion Tool Simplify pump maintenance: use one end to remove your old seal, then simply slip your new seal on the other end and push it flush into position. The tool cannot mar the surrounding metal surface of the pump housing. Remove a seal Fittings, Connectors, Tools 53 Seat a seal Description qty. cat.# price HPLC Piston Seal Insertion Tool ea Use the flat side of the Piston Seal Insertion Tool to seat a Waters face seal. HPLC ACCESSORIES Secure-Fit Fittings A good connection between HPLC components is necessary for trouble-free chromatography. Secure-Fit connectors from Restek and Selerity Technologies ensure a consistent, leak-free seal and they eliminate excess dead volume! An internal spring mechanism holds the capillary tubing at the proper depth in the female fitting. This seal is maintained while you finger-tighten the nut. These fittings are available in stainless steel or PEEK, and in a variety of tubing lengths and internal diameters. Stainless Steel Secure-Fit Fittings Single Connector Length 0.005" ID 0.007" ID 0.00" ID price 6cm cm cm cm cm Stainless Steel Secure-Fit Fittings Two Connectors Length 0.005" ID 0.007" ID 0.00" ID price 0cm cm cm cm Stainless Steel Secure-Fit Fitting PEEK Secure-Fit Fittings Single Connector Length 0.005" ID 0.007" ID 0.00" ID price 6cm cm cm cm PEEK Secure-Fit Fittings Two Connectors Length 0.005" ID 0.007" ID 0.00" ID price 0cm cm cm

56 54 Connectors, Tubing Accessories PEEK Tubing Elbows Tubing Elbows (90 and 80 ) are ideal for routing /6-inch PEEK tubing through your system. Simply snap the tubing into the elbow. Prevent pinching of PEEK tubing which can cause high pressure. Description qty. cat.# price PEEK Tubing Elbow 90 5-pk PEEK Tubing Elbow 80 5-pk Tubing Dressing Tool Opens stainless steel tubing bore and removes burrs. For /6-inch OD tubing or /8-inch OD tubing. HPLC ACCESSORIES Description qty. cat.# price /6" Tubing Dressing Tool ea Replacement Insert for /6" Tubing Dressing Tool ea /8" Tubing Dressing Tool ea Replacement Insert for /8" Tubing Dressing Tool ea. 209 Clean-Cut Tubing Cutter Burr-free, perpendicular cuts that will not distort the tubing OD or close the ID. Use on PEEK, Teflon, Tefzel, other polymeric tubing. Description qty. cat.# price Clean-Cut Tubing Cutter ea Replacement Blade for Clean-Cut Cutter ea For stainless steel tubing cutters, see our general catalog or website. Universal 0-32 PEEK Column Connectors and Plugs Universal PEEK Connectors allow easy installation of all /6-inch tubing, including stainless steel. See Improving Column Connections data on page 52. Description qty. cat.# price PEEK Column Connector (beige, round body) 0-pk PEEK Column Plug (black) 0-pk PEEK Fingertight Fittings (blue, flat-sided) 0-pk Rheodyne Style Nut and Ferrule Replacement long nut for connecting stainless steel tubing to a Rheodyne 6-port valve or other Rheodyne part. Description qty. cat.# price /6" Rheodyne Style Nut 0-pk /6" Rheodyne Style Ferrule 0-pk ValvTool Wrench The ValvTool is a time-saving device that provides easy access to many hard-to-reach Rheodyne or Valco valves. For /4-inch nuts. Description qty. cat.# price ValvTool Wrench ea. 2532

57 Tubing 55 Inert PEEK Tubing Replaces stainless steel, titanium, Teflon or Tefzel tubing. Less oxygen permeable and more temperature resistant (to 250 C) than Teflon or Tefzel tubing. Use with PEEK fingertight or flangeless fittings. Use to 7,000psi (48,263kPa). Description Color Code qty. cat.# price PEEK Tubing, /6" OD x " ID x m pink dash stripe 3-pk PEEK Tubing, /6" OD x 0.005" ID x 3m red stripe ea PEEK Tubing, /6" OD x 0.007" ID x 3m yellow stripe ea PEEK Tubing, /6" OD x 0.00" ID x 3m blue stripe ea PEEK Tubing, /6" OD x 0.020" ID x 3m orange stripe ea HPLC Stainless Steel Capillary Tubing 36 grade stainless steel. Precise pre-cut lengths. Smooth surface finish. Ultra clean. Whether you need to replace system tubing as part of your troubleshooting or are looking to reduce the dwell volume of your system as you move to narrower columns, Restek has the quality tubing in the lengths and IDs you need. Each ID is color-coded so it is easy to identify and replace correctly. HPLC ACCESSORIES /6" OD 0.005" ID (red) 0.007" ID (gray) 0.00" ID (blue) 0.020" ID (yellow) Length qty. cat.# price cat.# price cat.# price cat.# price 5cm 3-pk cm 3-pk cm 3-pk cm 3-pk Teflon Tubing Ideal for mobile phase inlet lines. Chemically inert. Use to 500psi (3,447kPa) and 80 C. Description qty. cat.# price Teflon Tubing, /8" OD x 0.063" ID x 3m (.6mm ID) 3m Teflon Tubing, /8" OD x 0.094" ID x 3m (2.4mm ID) 3m Tubing Clip Securely holds /6-inch or /8-inch tubing in beaker, flask, or bottle up to 4mm thick. Description qty. cat.# price Tubing Clip 5-pk. 2530

58 56 Flow Splitters, Static Mixers QuickSplit Post-Column Flow Splitters Fluid resistor technology eliminates adjustments to capillary tubing for optimizing split ratio. Wide range of interchangeable resistors available. Fixed Flow Splitters for HPLC & LC/MS Split ratio not affected by changes in viscosity or pressure. High operating pressure limit: 0,000psi (68,948kPa). Low dead volume - negligible effect on analyte bandwidth. Total flow: mL/min. HPLC ACCESSORIES did you know? We are pleased to offer HPLC accessory items from Analytical Scientific Instruments (ASI). ASI specializes in HPLC system components and parts that often exceed the original equipment manufacturer s performance. See page 45. Adjustable Flow Splitter Adjustable metering valve gives convenient control of split ratio. Split ratio not affected by changes in viscosity or pressure. High operating pressure limit: 5,000psi (34,474kPa). Low dead volume - negligible effect on analyte bandwidth. Total flow: mL/min. Description Split Ratio qty. cat.# price Binary Fixed Flow Splitter 00:0:00 ea :0:00 ea :0 ea :0 ea :0 ea Replacement Fixed Flow Splitter Resistor Set 00:0:00 ea :0:00 ea :0 ea :0 ea :0 ea Adjustable Flow Splitter 5: to 00: ea new! : to 20: ea : to 000: ea Replacement Adjustable Flow Splitter Resistor Set 5: to 00: ea : to 20: ea new! HyperShear HPLC Mixers Reduced baseline noise. Increased sensitivity. Improved gradient accuracy, for more reproducible results. Increased reaction efficiency in post-column derivatization. An efficient cross-flow shearing mechanism and interchangeable cartridges produce vortex shear mixing over a wide range of flows. Stainless steel or PEEK. HyperShear Static In-Line Mixers Stainless Steel PEEK Description qty. cat.# price qty. cat.# price µl In-Line Mixer ea ea µL In-Line Mixer ea. 264 ea µL In-Line Mixer ea ea µL In-Line Mixer ea ea µL In-Line Mixer ea ea Ternary Tee Mixer Description qty. cat.# price 25µL Ternary Tee Mixer ea. 2538

59 FlatLine Pulse Damper Rupture-proof, no diaphragm minimal risk of failure or leaks. Clean flush-out design no sample carryover. Low internal volume negligible effect on analyte bandwidth. Pulse Dampers, Backpressure Regulators 57 The ASI FlatLine Pulse Damper combines performance and reliability in a simple, easy-to-use housing. Standard 0-32 inlet and outlet ports allow quick connection into virtually any HPLC system. Solid core technology provides reliable long-term operation without the down time associated with ruptured or leaking membrane dampers µL internal volume at ambient pressure. Description qty. cat.# price FlatLine Pulse Damper ea MicroPulse Pulse Dampers Compact unit (2.5" x.5") can be placed almost anywhere. Small, 50µL dead volume at atmospheric pressure. PEEK unit can withstand pressures to 5,000psi (34,474kPa). 36 Stainless steel unit can withstand pressures to 6,000psi (4,369kPa). Improves system baseline stability while increasing the total system volume by only 50µL. The MicroPulse pulse damper is ideal for applications where minimizing the total system volume is critical. Stainless steel and PEEK options for a wide range of applications. HPLC ACCESSORIES Description qty. cat.# price MicroPulse Pulse Damper, Stainless Steel ea MicroPulse Pulse Damper, PEEK ea LO-Pulse Pulse Damper The LO-Pulse Pulse Damper is a patented, wide-dynamic-range 36 stainless steel device that smooths pulsations and maintains constant flow at system pressures up to 6,000psi (4,369kPa). The flow path volume is only 0.9mL and the path is efficiently swept, eliminating solvent memory effects when changing mobile phases. The LO-Pulse pulse damper also is available in a space-saving, economical kit that includes hardware for mounting the pulse damper on a bracket, or for installing feet on it for bench-top use. Description qty. cat.# price Model LP-2 LO-Pulse Pulse Damper ea Pulse Damper Kit kit 2503 LO-Pulse Pulse Damper Kit Backpressure Regulators Backpressure regulators can improve detector performance by preventing bubble formation in the detector flow cell. They also are useful in post-column reaction lines and between detectors and fraction collectors in preparatory work. Regulators are superior to more specific alternative solutions, like smallbore tubing, in which pressure varies with flow rate. Our end-of-line and flow-through backpressure regulators are adjustable to assure constant backpressure over a wide range of mobile-phase viscosities and flow rates. The end-of-line model is available with /4-28 plastic flange-type fittings or high-pressure /6-inch compression fittings; this design adjusts from 5 to 60psi (03 to 44kPa). The flow-through design has /6-inch compression fittings and is adjustable from 7 to 75psi (48 to 57kPa). Description qty. cat.# price A) Backpressure Regulator: end-of-line, /6-inch OD tubing, flanged ea B) Backpressure Regulator: end-of-line, high-pressure seat ea C) Backpressure Regulator: flow-through, 5µL internal volume ea AA B B C C

60 58 Column Hardware HPLC Column Tubing Restek tubing is manufactured from fine, chromatographic-grade 36 stainless steel. It is corrosion resistant, ultrasonically cleaned and passivated. We polish the tubing and promise a burr-free cut. You can use this tubing immediately there is no need for any additional treatment. HPLC ACCESSORIES 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price /4" OD Tubing 30mm mm mm mm mm mm /4-Inch HPLC Frits We manufacture our frits from fine, chromatographic-grade 36 stainless steel, and we offer sizes to fit most column and pore sizes. To choose the correct frit, check its pore size compatibility with the particle size of the packing in the column (see chart at left). If the packing has a smaller particle size than the pore size of the frit, the packing can clog the frit. Packing particle size 3 4µm 5 20µm Use this pore size: 0.5µm 2.0µm ID Pore Size qty. cat.# price 4.6mm 2.0µm 0-pk mm 0.5µm 0-pk mm 2.0µm 0-pk mm 0.5µm 0-pk mm 2.0µm 0-pk mm 0.5µm 0-pk Column End-Fittings /4-inch end-fittings are compatible with Valco and Parker fittings. Description qty. cat.# price Column End-Fitting for 4.6mm Column ea Column End-Fitting Flat Bottom ea Empty Chromatography Columns 36 stainless steel tubing complete with end-fittings, frits, nuts, and ferrules. Preassembled prior to shipment, unless otherwise requested. Internal /6-inch seats are compatible with Valco and Parker fittings. 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price /4" OD Tubing 30mm mm mm mm mm mm

61 Restek Pack in a Box Kit, HPLC Organizers 59 Restek Pack in a Box Kit Restek s new Pack in a Box Kit is a complete column-packing system. Everything you need to pack your own analytical HPLC columns is included: pump, reservoir, column hardware, packing material, and system control software (computer not included). Detailed packing instructions and a video also are included. The packing pump is electric, so no pressurized air is required. new! Description qty. cat.# price Pack in a Box Kit kit HPLC Organizers HPLC ACCESSORIES LCLocker HPLC Organizer Deluxe BenchBooster Organizer Mini phperch Storage Unit TopLoader BalanceBank Storage Unit Bill Grove Instrument Innovations + year of service! HPLC 30-Column Storage Cabinet Book Holders Open Supply Bins, 3-bin unit Glove Box Dispensers Description dimensions qty. cat.# price LCLocker 24 x 2 x 6" ea BenchBooster 24 x 7 x 2" ea Mini phperch 3 x 2 x 6" ea TopLoader 2 x 2 x 7" ea Column Cabinet 7 3 /8 x 5 x 2 7 /8" ea Book Holder, Small 0.75" ID ea. 255 Book Holder, Large.5" ID ea Open Supply Bin, 4-bin Unit 24 x 2 x 0" ea Open Supply Bin, 5-bin Unit 2 x 6 x 0.5" ea Open Supply Bin, 3-bin Unit 2 x 7.5 x 2" ea Glove Box Dispenser, Single 5 3 /8 x /2 x 4 /2" ea Glove Box Dispenser, Double 0 3 /8 x /2 x 4 /2" ea Glove Box Dispenser, Triple 5 3 /8 x /2 x 4 /2" ea *Please note: Accessories and supplies in photographs are not included.

62 60 Tech Tips HPLC Pump Pressure Conversion Table Pressure psi atm kg/cm 2 torr kpa bar inches Hg psi = atm = kg/cm 2 = torr = kpa = bar = in Hg = HPLC ACCESSORIES Acetone Acetonitrile Dimethyl formamide Dimethyl sulfoxide Dioxane Ethanol Isopropyl alcohol Methanol Tetrahydrofuran Water Benzene Butanol Carbon tetrachloride Chloroform Cyclohexane,2-Dichloroethane Dichloromethane Ethyl acetate Ethyl ether Heptane Hexane Isooctane Methyl tert-butyl ether Methyl ethyl ketone Pentane Toluene Xylene Multiply units in the left-most column by the conversion factors listed in the columns to the right. e.g., 0 psi x = 0.68atm 0 bar x = inches Hg Acetone Acetonitrile Dimethyl formamide Dimethyl sulfoxide Dioxane Solvent Miscibility and Solubility Ethanol Isopropyl alcohol Methanol Tetrahydrofuran Water Benzene Butanol Carbon tetrachloride Chloroform Cyclohexane,2-Dichloroethane Dichloromethane Ethyl acetate Ethyl ether Miscible/Water Miscible Solvents Miscible Solvents Immiscible Solvents Heptane Hexane Isooctane Methyl tert-butyl ether Methyl ethyl ketone Pentane Toluene Xylene

63 mm Vials, 9mm Vials 6 Crimp-Top Vials, 2.0mL, 2 x 32mm, mm Crimp Finish White graduated marking spots are a standard feature unless otherwise noted. Crimp-Top Vials, Snap Seal Style 2 x 32mm, mm Crimp Description 00-pk. 000-pk. 2.0mL Clear Glass Vial w/white Graduated Marking Spot mL Amber Glass Vial w/white Graduated Marking Spot mL Clear Glass Vial without Graduated Marking Spot mm Aluminum Crimp Seals with Septa Description 00-pk. 500-pk. 000-pk. Silver Seal, PTFE/Natural Rubber Septa Blue Seal, PTFE/Red Rubber Septa Green Seal, PTFE/Red Rubber Septa Red Seal, PTFE/Red Rubber Septa Yellow Seal, PTFE/Red Rubber Septa Mixed Colors, PTFE/Rubber Septa 2724 Silver Seal, PTFE/Silicone Septa Blue Seal, PTFE/Silicone Septa Green Seal, PTFE/Silicone Septa Red Seal, PTFE/Silicone Septa Yellow Seal, PTFE/Silicone Septa Mixed Colors, PTFE/Silicone Septa 2725 Silver Seal, PTFE/Silicone/PTFE Septa for more info see page 62 for electronic crimpers and decappers. HPLC ACCESSORIES Convenience Kits: Vials, Caps, & Septa Vials packaged in a clear-lid tray. Caps with septa packaged in a plastic bag. Description 00-pk. 000-pk. 2.0mL Clear Vial, deactivated, PTFE/Natural Rubber Silver Seal mL Amber Vial, deactivated, PTFE/Natural Rubber Silver Seal mL Clear Vial, untreated, PTFE/Natural Rubber Silver Seal mL Amber Vial, untreated, PTFE/Natural Rubber Silver Seal mL Clear Vial, untreated, PTFE/Silicone Silver Seal mL Amber Vial, untreated, PTFE/Silicone Silver Seal mm Short-Cap, Screw-Thread Vials, 2 x 32mm, 9mm Opening Fit all 2.0mL, 2 x 32mm, Crimp-Top Vial Based Autosamplers. Shorter screw cap allows the robotic autosampler to function properly. Wider opening prevents syringe problems. Dimensions are effectively identical to the crimp-top vial. Variety of colors and liner material. 9mm Short-Cap, Screw-Thread Vials, 2 x 32mm, 9mm Opening Description 00-pk. 000-pk. 2.0mL Clear Vial, w/white Graduated Marking Spot mL Amber Vial, w/white Graduated Marking Spot mL Clear Vial, without Graduated Marking Spot mm Thread Pre-Assembled Short-Cap, Screw-Vial Closures Description 00-pk. 500-pk. 000-pk. Blue Cap, PTFE/Red Rubber Liner Mixed Colors, PTFE/Red Rubber Liner Blue Cap, PTFE/Silicone Liner Green Cap, PTFE/Silicone Liner Yellow Cap, PTFE/Silicone Liner Mixed Colors, PTFE/Silicone Liner Blue Cap, PTFE/Silicone/PTFE Liner Black Cap, PTFE/Silicone/PTFE Liner Mixed Colors, PTFE/Silicone/PTFE Liner Cap 24670

64 62 Crimpers and Decappers Safer than removing caps with pliers, knife or screwdriver blade! Aluminum Seal Crimpers and Decappers The crimper is adjustable for optimized sealing performance and is comfortable enough for prolonged use. If you need to save, transfer, or dispose of your samples, our decapper allows you to remove a crimptop cap safely and easily. Far safer than removing caps with pliers, knife or screwdriver blades, etc. If you aren t currently using a seal decapper, we recommend you order one today! HPLC ACCESSORIES Decapper Crimper Description qty. cat.# price 8mm Crimper ea mm Decapper ea mm Crimper ea. 270 mm Decapper ea. 27 3mm Crimper ea mm Decapper ea mm Crimper ea mm Decapper ea Hand-held Rechargeable Crimpers and Rechargeable Decappers Easy to use; comfortable grip. Hundreds of operations from one charge. Adjustable crimping force. All kits include battery charger. Powered by a standard Black and Decker Versapak battery, these electronic tools for mm or 20mm caps will cycle hundreds of times on a single charge. The cycle is controlled precisely, through an internal counter, and is adjusted with two buttons on the side of the case. The tools fit comfortably in the hand and weigh approximately 600 grams. The jaws can be positioned easily around closely-spaced vials in standard autosampler trays. Each kit includes the tool, a Versapak Gold rechargeable battery, which uses environmentally friendly NiMH technology, and a charger. Recharging generally takes 6-9 hours. Decapping has never been easier! one... two... three! The Electronic Crimper fits around vials in standard autosampler trays. The adjustable setting provides a precision crimp, vial after vial. Description qty. cat.# price mm Electronic Crimper (0 volt battery charger) kit mm Electronic Crimper (220 volt European battery charger) kit EUR mm Electronic Crimper (220 volt UK battery charger) kit UK mm Electronic Decapper (0 volt battery charger) kit mm Electronic Decapper (220 volt European battery charger) kit EUR mm Electronic Decapper (220 volt UK battery charger) kit UK 20mm Electronic Crimper (0 volt battery charger) kit mm Electronic Crimper (220 volt European battery charger) kit 2285-EUR 20mm Electronic Crimper (220 volt UK battery charger) kit 2285-UK 20mm Electronic Decapper (0 volt battery charger) kit mm Electronic Decapper (220 volt European battery charger) kit EUR 20mm Electronic Decapper (220 volt UK battery charger) kit UK

65 WISP Style Vials 63 Screw-Thread Step Vials, 4.0mL, WISP 48 Style, 3mm/425 Screw-Thread WISP 48 Screw-Thread Step Vials (5 x 45mm, 3/425 Screw-Thread) Description 00-pk. 000-pk. 4.0mL Clear Step Vial, White Grad. Marking Spot mL Amber Step Vial, White Grad. Marking Spot Caps and Septa Description 00-pk. 000-pk. Top Hat, PTFE/Silicone Septa Pre-Assembled, Red PTFE/Silicone, 0.065" Caps, Black, Polypropylene, Open-Hole Septa, Red PTFE/Silicone, 0.040" Septa, Red PTFE/Silicone, 0.060" Limited Volume Inserts Description 00-pk. 000-pk. 500µL, Glass, WISP, Flat Bottom* *Also fit 4.0mL Snap Seal Vials (see our general catalog or website). 4.0mL Clear Step Vial HPLC ACCESSORIES Shell Vials,.0mL, WISP 96 Style, 8 x 40mm WISP 96 Style, 8 x 40mm Shell Vials Description 200-pk. 000-pk..0mL Clear Vial mL Amber Vial mL Polypropylene Vial µL Limited Volume Polypropylene Vial mm Starburst Plugs Description 200-pk. 000-pk. Snap Plug, Clear Polyethylene Snap Plug, Conical, Clear Polyethylene Snap Plug, Silicone Lined, Clear Polyethylene Limited Volume Inserts for Shell Vials Description 00-pk. 000-pk. 250µL Glass, Big Mouth Insert w/ Bottom Spring

66 64 LC Autosampler Syringes Syringes for Agilent 090 & 00 LC Autosamplers SGE Syringes 25μL, /4-32 UNEF Thread SGE Restek Volume Model cat.# qty. cat.# price 25µL 25D-HP090-GT ea µL 250D-HP090-GT ea HPLC ACCESSORIES Syringe for Hitachi LC Autosamplers SGE Syringe 500μL, M0 X Thread SGE Restek Volume Model cat.# qty. cat.# price 500µL 500C-HITACH ea Syringes for PerkinElmer LC Autosamplers SGE Syringes 250μL, /4-28 UNF Thread SGE Restek Volume Model cat.# qty. cat.# price 50µL 50D-CX-GT ea µL 00D-CX-GT ea µL 250D-CX-GT ea µL 500D-CX-GT ea ml MD-C-GT ea Syringes for Waters WISP LC Autosamplers SGE Syringes 250μL, /4-28 UNF Thread SGE Restek Volume Model cat.# qty. cat.# price 25µL 25D-WISP ea µL 250D-WISP ea Syringes for Waters WISP LC Autosamplers Teflon PTFE-tipped plungers Hamilton Syringes Hamilton Restek Volume Model cat.# qty. cat.# price 25µL ea µL ea

67 LC Autosampler Syringes / Analytical Reference Materials 65 Syringes for CTC/LEAP LC Autosamplers Hamilton Syringes Hamilton 0μL LC Autosampler Syringe Volume Needle Needle Needle Point Hamilton Restek Term. Gauge Length Style Model cat.# qty. cat.# price 0µL N 22s 2"/5mm 3 70N ea µL N 22s 2"/5mm 3 702N ea µL N 22s 2"/5mm 3 70N Slim Line* ea µL N 22 2"/5mm 3 70N ea µL N 22 2"/5mm 3 725N ea µL N 22 2"/5mm 3 750N ea *Barrel O.D. = 6.7mm; all other 25µL and 00µL syringes have a 7.9mm barrel O.D. SGE Syringes SGE 0μL LC Autosampler Syringe Volume Needle Needle Needle Point SGE Restek Term. Gauge Length Style Model cat.# qty. cat.# price 0µL F 22s 2"/5mm LC 0F-CTC-LC ea µL F 22s 2"/5mm LC 25F-C/T-LC ea µL* R 22s 2"/5mm LC 25R-C/T-GT-LC ea µL* F 22s 2"/5mm LC 50F-C/T-GT-LC ea µL* R 22s 2"/5mm LC 00R-C/T-GT-LC ea µL* F 22s 2"/5mm LC 500F-CTC-GT-LC(0.4) ea *Gas-Tight syringe. new! new! HPLC ACCESSORIES Analytical Reference Materials HPLC Normal Phase Test Mix # Routine analysis using this mix can assist in determining the need to perform column and/or system maintenance. benzene.00mg/ml benzyl alcohol 3.00 benzaldehyde methoxybenzyl alcohol 2.00 In hexane, ml/ampul cat. # (ea.) No data pack available. HPLC Reversed Phase Test Mix # Routine analysis using this mix can assist in determining the need to perform column and/or system maintenance. benzene 3.00mg/mL naphthalene 0.50 uracil 0.02 biphenyl 0.06 In methanol:water (75:25), ml/ampul cat. # (ea.) No data pack available. HPLC Performance Test Mix The National Institute of Standards and Technology (NIST) formulated a mixture that is highly effective for characterizing HPLC columns for efficiency, void volume, methylene selectivity, retentiveness, and activity toward chelators and organic bases. Results can be used for column classification, column selection, monitoring column performance over time, or for quality control. We test our material against the NIST 870 standard. amitriptyline quinizarin 94 hydrochloride 2800µg/mL toluene 400 ethylbenzene 700 uracil 28 In methanol, ml/ampul cat. # 3699 (ea.) HPLC OQ Linearity Test Mix Kit Linear detector responses to concentration variations are an important part of operation qualification (OQ) for HPLC instruments. Our kit of five aqueous solutions of caffeine can be used to generate simple plots of UV response versus concentration. Certificate of Analysis includes caffeine concentration, calculated variance in preparing each mixture, a linearity plot, and coefficient of determination (r²) for the linear plot. Caffeine at 5.0, 25.0, 25.0, 250.0, µg/ml in water in a five ampul kit. ml each of these mixtures. cat. # 3805 (kit) No data pack available. Quantity discounts not available. Carbohydrate HPLC Performance Check Mix Performance qualification (PQ) determines the precision of the HPLC system. Our performance check mix for HPLC / RI consists of five simple sugars in varied concentrations. We prepare the reference material in water, lyophilize it, and pack it dry for enhanced stability. glucose 2.0mg maltose 4.5 fructose 2. sucrose 4.0 lactose 4.4 Dry components in 4mL screw-cap vial. Reconstitute in ml acetonitrile:water (75:25) to 2.0, 2., 4.4, 4.5, 4.0 mg/ml, respectively. cat. # 3809 (ea.) No data pack available.

68 66 Nucleotides, Nucleosides, Nucleic Acid Bases Nucleotides, Nucleosides, and Nitrogenous Bases on Ultra IBD Uracil, Uridine, UTP, UDP, and UMP on Ultra IBD HPLC APPLICATIONS BIOCHEMICAL/CLINICAL Conc. (µg/ml). CDP CMP 3 3. ATP 7 4. ADP cytidine TMP AMP guanine guanosine adenine 38. adenosine Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 0 Inj.: 20µL Solvent: 20mM ammonium acetate, ph min. LC_028 Thymine, Thymidine, TTP, TDP, and TMP on Ultra IBD mM ammonium acetate, ph 5.8:methanol (97.5:2.5).0mL/min. Temp.: 35 C 260nm Conc. (µg/ml). TTP TDP TMP thymine thymidine 38 Inj.: 20µL Solvent: 20mM ammonium acetate, ph 5.8 Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 20mM ammonium acetate, ph 5.8: methanol (97.5:2.5).0mL/min. Temp.: 35 C 260nm min. LC_033 Inj.: Conc. Solvent: Conc. (µg/ml). UTP UDP UMP uracil uridine 230 Inj.: 20µL Solvent: 20mM ammonium acetate, ph 5.8 Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 20mM ammonium acetate, ph 5.8: methanol (97.5:2.5).0mL/min. Temp.: 35 C 260nm Nucleic Acid Bases on Allure PFP Propyl. cytosine 2. uracil 3. guanine 4. thymine 5. adenine 2 3.0µL 00µg/mL each 20 mm ammonium acetate, ph 5.0 Note: -3 drops of ammonium hydroxide added to keep guanine in solution Column: Allure PFP Propyl Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 4 Temp.: 20mM ammonium acetate, ph 5.0: methanol (85:5, v/v).0ml/min. ambient 260nm min. LC_ min. LC_037

69 Purines/Pyrimidines, Amino Acids, Cocaine 67 Purines/Pyrimidines on Ultra PFP min. Conc. (mg/ml). tyrosine phenylalanine.6 3. tryptophan 0.4 Inj.: 20µL Solvent: mobile phase A LC_036 Aromatic Amino Acids on Ultra Aqueous C8 Column: Ultra Aqueous C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å Mobile phases: A: 50mM potassium phosphate, ph 2.5 B: acetonitrile 5% - 20% B: 0-5 min. 20% - 5% B: 5-6 min. Hold at 5% B: 6-3 min..0ml/min. Temp.: 30 C 254nm Searching for a chromatogram? cytosine 2. 5-methylcytosine 3. uracil 4. uric acid 5. hypoxanthine 6. xanthine 7. thymine 8. purine 9. theobromine 0. ethyluracil. 6-methylpurine 2.,7-dimethylxanthine 3. theophylline 4. propyluracil Inj.: 0µL Conc.: 00µg/mL each Solvent: mobile phase Column: Ultra PFP Cat. #: Dimensions: 250 x 4.6mm Pore size: 00Å Temp.: 0.00% formic acid in water: methanol (50:50, v/v).0ml/min. ambient 260nm Cocaine and Ecgonine Methyl Ester on Allure PFP Propyl. EME (ecgonine methyl ester) 2. COC (cocaine) Inj.: 0µL Conc.: µg/ml Solvent: water Temp.: 4 C Column: Allure PFP Propyl Cat. #: Dimensions: 30 x 2.mm Pore size: 60Å LC_026 Column temp.: XIC of +MRM (3 pairs) for / 82.0 amu.30e3 cps 5mM ammonium formate, ph 3.0: acetonitrile (0:90, v/v) 0.6mL/min. ambient Applied Biosystems API 3200 LC/MS/MS System turbo ion spray, ESI Interface: Interface temp.: 50 C Ion mode: positive ESI probe voltage: 5000V Orifice: + 7V Ring: + 265V Collision gas: nitrogen Collision gas pressure: 2.2 mtorr Collision gas energy: 28 ev (COC) 26 ev (EME) Electron multiplier: 200 volts Auxilary gas flow: 7000cc/min. Nebulizer gas setting: 5lb/in.² Curtain gas setting: 2lb/in.² HPLC APPLICATIONS BIOCHEMICAL/CLINICAL min. XIC of +MRM (3 pairs) for / 82.0 amu 2.35e3 cps min. LC_ min. Data Courtesy of Shane Needham, Pfizer Inc.

70 68 Proteins, Peptides, Catecholamines Protein Mix on Viva C8 Viva Wide Pore C8 (cat.# ) Note excellent peak shapes and resolution on the Viva column! HPLC APPLICATIONS BIOCHEMICAL/CLINICAL mfr. H mfr. D mfr. C mfr. F Viva Manufacturer A Manufacturer B Manufacturer C Manufacturer D Peptide Mix on Viva C8 LC_ LC_BC0386 Inj.: 20µL peptide test mix Column: Viva C8 Wide Pore Cat.#: Dimensions: 50 x 2.mm Pore size: 300Å. val-tyr 2. val-tyr-val 3. meth enkephalin 4. val-4-angiotensin III 5. angiotensin II, III 6. leucine enkephalin 7. angiotensin I A % trifluoroacetic acid in water (ph 2.0) B % TFA in acetonitrile, 0% B to 40% B in 20 min. 0.2mL/min. 24nm * High levels of trace metals (~00ppm) in the silica enhance this separation. * 7 Peak List Ret. Time (min.). ribonuclease A.3 2. cytochrome c holo-transferrin apomyoglobin Inj.: 20µL Conc.: 0.08mg/mL each protein Sample diluent: 0.0% TFA in water / 0.0% TFA in acetonitrile, 80:20, v/v Sample temp.: 25 C Columns: Wide Pore C8 Dimensions: 50 x 2.mm Pore size: 300Å Temp.: Catecholamines on Allure PFP Propyl LC_BA0349 A: 0.0% TFA in water, B: 0.0% TFA in acetonitrile, 20% B to 70% B in 30 min. 0.20mL/min. 25ºC (or ambient) 24nm Peak List Retention Time (min.). norepinephrine levodopa epinephrine tyrosine dopamine.3 Inj.: 0µL Conc.: 40µg/mL each component Sample diluent: mobile phase Sample temp.: ambient Column: Allure PFP Propyl Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 50mM ammonium phosphate (ph 3.0, adjusted with formic acid) ml/min. Temp.: 35 C 266nm

71 Carbonyls by CARB Method 004 on Allure AK Aldehydes, Ketones new! 69 dinitrophenylhydrazine (DNPH) derivatives of aldehydes/ketones Inj.: 0µL Conc.: 3µg/mL each analyte, as aldehyde/ketone Sample diluent: acetonitrile Column: Allure AK Cat.#: Dimensions: 200 x 4.6mm Pore size: 60Å A) water : B):acetonitrile Time (min.) %B mL/min. Temp.: 30 C 360nm Aldehydes and Ketones (DNPH derivatives) on Ultra C8 (40 C) DNPH derivatives of:. formaldehyde 2. acetaldehyde 3. acetone 4. acrolein 5. propionaldehyde 6. crotonaldehyde 7. MEK 8. methacrolein 9. butyraldehyde 0. benzaldehyde. valeraldehyde 2. m-tolualdehyde 3. hexanaldehyde LC_EV0393 Ret. Time Peak (min.) DNPH derivative of:. formaldehyde acetaldehyde acrolein acetone propionaldehyde crotonaldehyde methacrolein butyraldehyde methylethylketone benzaldehyde valeraldehyde m-tolualdehyde hexaldehyde.36 Inj.: 0µL Conc.: 3.0µg/mL each derivative Column: Ultra C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å Solvent A: water Solvent B: acetonitrile Solvent C: tetrahydrofuran 50-30% A/30-65% B/20-5% C, 0-5 min. Flow rate:.5ml/min. 365nm Temp.: 40 C HPLC APPLICATIONS BIOCHEMICAL/CLINICAL min. LC_0036

72 70 Explosives EPA 8330B Explosives on Ultra C8 HPLC APPLICATIONS ENVIRONMENTAL Ret. Time (min.). HMX RDX ,3,5-TNB ,3-DNB ,5-DNA NB tetryl ,4,6-TNT NG A-4,6-DNT A-2,6-DNT ,4-DNT ,6-DNT NT NT NT PETN 2.08 EPA 8330B Explosives on Pinnacle II Biphenyl Ret. Time (min.). HMX RDX NG ,5-DNA NB A-2,6-DNT A-4,6-DNT ,3-DNB NT PETN NT NT ,6-DNT ,3,5-TNB ,4-DNT tetryl ,4,6-TNT 20.7 Column: Ultra C8 Cat.#: Dimensions: 250mm x 4.6mm Pore size: 00Å 50µg/mL each compound diluted in acetonitrile 8330 Calibration Mix # (cat.# 3450) 8330 Calibration Mix #2 (cat.# 345) PETN Standard (cat.# 3600) 3,5-dinitroaniline Standard (cat.# 366) Nitroglycerin Standard (cat.# 3498) Inj.: 0µL water:methanol (44:56 v/v).0ml/min. Temp.: 30 C UV 20nm Column: Pinnacle II Biphenyl Cat.#: Dimensions: 250mm x 4.6mm Pore size: 0Å LC_EV µg/mL each compound diluted in acetonitrile 8330 Calibration Mix # (cat.# 3450) 8330 Calibration Mix #2 (cat.# 345) PETN Standard (cat.# 3600) 3,5-dinitroaniline Standard (cat.# 366) Nitroglycerin Standard (cat.# 3498) Inj.: 0µL water:methanol (44:55 v/v).2ml/min. Temp.: 30 C UV 20nm LC_EV0429

73 Herbicides, Pesticides 7 Herbicides (Phenylurea) on Ultra C8 Herbicides on Ultra Aqueous C8. 2,4-D 2. Silvex (2,4,5-TP) 3.,4-dichlorobenzene (IS) Fast isocratic analysis! Conc. (µg/ml or ppm). tebuthiuron 2 2. thidiazuron 2 3. monuron 5 4. fluometuron 2 5. diuron 2 6. propanil 2 7. siduron 2 8. linuron 2 9. carbazole 5 0. diflubenzuron 2 2 Inj.: 0µL Conc.: 0ppm each Solvent: 0.05% phosphoric acid : acetonitrile, 50:50 Column: Ultra Aqueous C8 Cat. #: Dimensions: 50 x 4.6mm Particle size: 3µm Pore size: 00Å 0.05% phosphoric acid : acetonitrile, 60:40.0mL/min. Temp.: ambient 225nm 3 LC_039 Inj.: 20µL Conc.: see peak list Sample diluent: acetonitrile Column: Ultra C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å A: 25mM phosphate buffer, ph 2.5 B: acetonitrile Time (min.) %B ml/min. Temp.: ambient 245nm Diflubenzuron (Pesticide) on Ultra IBD. diflubenzuron 2. N,N'-bis-(chlorobenzyl)urea Inj.: 0µL Conc.: mg/ml Solvent: water:acetonitrile (:, v/v) Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Particle size: 3µm Pore size: 00Å Solvent A: Flow rate: Temp.: water:acetonitrile (45:55, v/v) 65% 0.M ammonium acetate ph 6.0.0mL/min. ambient 260nm 2 HPLC APPLICATIONS ENVIRONMENTAL min. LC_ min. LC_008

74 72 Herbicides Phenoxyacid Herbicides on Ultra Aqueous C8 new! HPLC APPLICATIONS ENVIRONMENTAL Mix A and Mix B. picloram 2. chloramben 3. 4-nitrophenol 4. dicamba 5. bentazon 2,4-dichlorophenyl acetic acid (surr.) (not included) 7. 2,4-D 8. MCPA 9. 3,5-dichlorobenzoic acid 0. MCPP. dichlorprop 2. 2,4,5-T 3. 2,4-DB 4. 2,4,5-TP (Silvex) 5. acifluorfen,4-dichlorobenzene (int. std.) (not included) 7. dinoseb 8. pentachlorophenol Phenoxyacid Herbicides on Allure Basix Mix A and Mix B. picloram 2. chloramben 3. 4-nitrophenol 4. dicamba 5. bentazon 2,4-dichlorophenyl acetic acid (surr.) (not included) 7. 2,4-D 8. MCPA 9. 3,5-dichlorobenzoic acid 0. MCPP. dichlorprop 2. 2,4,5-T 3. 2,4-DB 4. 2,4,5-TP (Silvex) 5. acifluorfen,4-dichlorobenzene (int. std.) (not included) 7. dinoseb 8. pentachlorophenol Chlorinated Acids by HPLC, Mix A (cat.# 3243), or Chlorinated Acids by HPLC, Mix B (cat.# 32430) 000µg/mL each component in acetonitrile Inj.: 0µL Conc.: 0ppm each analyte Sample diluent: acetonitrile Column: Ultra Aqueous C8 Cat.#: Dimensions: 50 x 4.6mm Pore size: 00Å Temp.: LC_EV0356 LC_EV0355 A) 0.05% H3PO4 B) acetonitrile Time (min.) %B mL/min. ambient 225nm new! Chlorinated Acids by HPLC, Mix A (cat.# 3243), or Chlorinated Acids by HPLC, Mix B (cat.# 32430) 000µg/mL each component in acetonitrile Inj.: 0µL Conc.: 0ppm each analyte Sample diluent: acetonitrile Column: Allure Basix Cat.#: Dimensions: 50 x 4.6mm Pore size: 60Å Temp.: A) 0.05% H3PO4 B) acetonitrile Time (min.) %B mL/min. ambient 225nm LC_EV0357 LC_EV0358

75 Herbicides, Pesticides 73 Herbicides on Allure C8 Ret. Time (min.). cyanazine methabenzthiazuron chlortoluron secbumeton isoproturon diuron linuron flusilazole ethofumesate neburon pendimethalin 3.46 LC_0309 Pesticides (Carbamates) on Ultra Carbamate Compound Ion Cone V. aldicarb sulfone V 2. aldicarb sulfoxide V 3. oxamyl 237.2* 0V 4. methomyl V 5. 3-hydroxycarbofuran V *Ammonium adduct (all other are [M+H] + ions) 3-hydroxycarbofuran oxamyl methiocarb aldicarb sulfone carbofuran propoxur aldicarb sulfoxide carbaryl Compound Ion Cone V 6. aldicarb 9.2 8V 7. propoxur V 8. carbofuran V 9. carbaryl V 0. methiocarb V Inj.: Conc.: Diluent: 5µL custom mix approx. 20µg/mL each acetonitrile Column: Allure C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å Temp.: A: water B: acetonitrile Time (min.) %B mL/min. ambient 220nm Inj.: 0µL Conc.: 00µg/mL each component Column: Ultra Carbamate Cat. #: Dimensions: 00 x 4.6mm Particle size: 3µm Pore size: 00Å Temp.: MSD Detector: Mode: Capillary V: 3.50 Extractor: 4.0 Ion energy: 0.4 Multiplier: 650 Source temp: 00 C A: 90% water: 0% methanol with 0mM ammonium formate B: 90% acetonitrile: 0% methanol with 0 mm ammonium formate Time (min.) %B 0.0 0% 5 90% 20 90%.0mL/min. (approx. 0.75mL/min. to UV detector, 0.25mL/min. to MSD) ambient 220nm; MSD Micromass ZMD ESI+ Desolv. temp: 250 C Gas flow: 490 L/hr. HPLC APPLICATIONS ENVIRONMENTAL aldicarb methomyl LC_0308

76 74 Pesticides Pesticides on Allure C8 HPLC APPLICATIONS ENVIRONMENTAL. desisopropyl atrazine 2. desethyl atrazine 3. simazine 4. terbuthylazine desethyl 5. cyanazine 6. methabenzthiazuron 7. chlortoluron 8. atrazine 9. secbumeton 0. isoproturon. diuron 2. terbumeton 3. terbuthylazine 4. linuron 5. flusilazole 6. ethofumesate 7. neburon Pesticides on Allure PFP Propyl. desisopropyl atrazine 2. desethyl atrazine 3. simazine 4. terbuthylazine desethyl 5. cyanazine 6. atrazine 7. methabenzthiazuron 8. secbumeton 9. chlortoluron 0. isoproturon. terbumeton 2. diuron 3. terbuthylazine 4. linuron 5. ethofumesate 6. flusilazole 7. neburon LC_030 Inj.: 5µL Conc.: approx. 0ppm each pesticide Solvent: acetonitrile Column: Allure C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å Mobile phase A: 20mM K2HPO4, ph 7.0 B: acetonitrile Time (min.) %B mL/min. Temp.: ambient 220nm Inj.: 5µL Conc.: approx. 0ppm each pesticide Solvent: acetonitrile Column: Allure PFP Propyl Cat. #: Dimensions: 50 x 4.6mm Particle size: 3µm Pore size: 60Å Mobile phase A: 20mM K2HPO4, ph 7.0 B: acetonitrile Time (min.) %B ml/min. Temp.: ambient 220nm LC_032

77 Herbicides 75 Paraquat and Diquat on Ultra Quat 2 Peak time (min.). diquat paraquat 8.8 min. diquat 308nm 00µg/mL 40µg/mL 20µg/mL Paraquat and Diquat on Ultra Quat Fast, sensitive LC/MS/MS analysis! paraquat dichloride [CAS# ] diquat dibromide [CAS # ] LC_EV0383 Inj.: 20µL Conc.: 20ppm each component (above); 20µg/mL, 40µg/mL, and 00µg/mL each component at 308nm (left) and at 257nm (right) Sample diluent: water Column: Ultra Quat Cat. #: Dimensions: 50 x 4.6mm Ultra Quat Reagent Solution (cat.# 3244): acetonitrile, 95:5 (v/v).0 ml/min. Temp.: 27 C Peak List. diquat 2. paraquat *Data courtesy of Houssain El Aribi, Ph.D., LC/MS Product and Application Specialist, MDS SCIEX, 7 Four Valley Drive, Concord, Ontario, Canada, L4K 4V8 257nm (paraquat) 308nm (diquat) 00µg/mL 40µg/mL 20µg/mL Inj.: 0µL Conc.: 5µg/mL each component Sample diluent: DI water Sample temp.: ambient Column: Ultra Quat Cat.#: Dimensions: 50 x 2.mm Particle size: 3µm Pore size: 00Å paraquat 257nm LC_032 0mM heptafluorobutyric acid:acetonitrile, 95:5 (v/v) 0.3mL/min. Temp.: ambient Applied Biosystems API 3200 Interface: electrospray Ion Mode: positive Temp.: 600 C Ion Spray voltage: 5500V Collision exit potential: 3V Curtain Gas : 5psi (03kPa) Gas supply : 70psi (483kPa) Gas supply 2: 60psi (44kPa) Quantitation: (MRM) Q/Q3: unit resolution Dwell time: 200 ms Precursor Ion Fragment Ion DP Collision Energy (amu) (amu) (V) (ev) diquat, paraquat, 93 (2+) min. new! HPLC APPLICATIONS ENVIRONMENTAL

78 76 Polynuclear Aromatic Hydrocarbons Polynuclear Aromatic Hydrocarbons (PAHs) on Pinnacle II PAH 6 HPLC APPLICATIONS ENVIRONMENTAL Inj.: 5µL Conc.: see peak list Sample Diluent: ::2 methylene chloride: methanol:acetonitrile Sample Temp.: ambient Column: Pinnacle II PAH Cat.#: Dimensions: 50mm x 3.2mm Particle size: 4µm Pore size: 0Å A: purified water B: acetonitrile Time (min.) %B Temp.:.2mL/min. ambient 254nm Time (min) Conc. (µg/ml). naphthalene acenaphthylene acenaphthene fluorene phenanthrene anthracene fluoranthene pyrene benzo(a)anthracene chrysene 250. benzo(b)fluoranthene benzo(k)fluoranthene benzo(a)pyrene dibenzo(a,h)anthracene benzo(ghi)perylene indeno(,2,3-cd)pyrene 250 LC_EV0442 free literature Environmental HPLC Applications Columns Reference Materials Restek HPLC columns support environmental HPLC applications with rapid analysis times and effective analyte resolution. Sample turn-around can be 50% faster, or greater, than with alternative columns. In addition, we prepare analytical reference materials and sample preparation products for these applications. Analyses described in this 8-page publication include carbamates, carbonyls, explosives, paraquat/diquat, phenoxyacid herbicides, and polyaromatic hydrocarbons. Call Restek at or , ext. 5, or contact your Restek representative, to request your free copy! lit. cat.# 5974A (domestic) lit. cat.# 5974A-INT (international)

79 Dietary Supplements: Plant Extracts 77 Allicin in Garlic Tablets on Pinnacle II C8 Phenethyl Glucosinolate on Ultra Aqueous C8. allicin Inj.: 20µL Conc.:.2mg/mL garlic tablet in water Solvent: deionized water Column: Pinnacle II C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å water:methanol (50:50 v/v) 0.8 ml/min. Temp.: 30 C 240nm min. LC_074 Phenolics in Echinacea on Pinnacle II C8 Inj.: 0µL Conc.: 6.7 mg/ml Echinacea capsule contents in sample diluent Solvent: ethanol:water (70:30, v/v) Sample temp.: 25 C Column: Pinnacle II C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å A = 0.% phosphoric acid B = acetonitrile Time (min.) %B mL/min. Temp.: 35 C 330nm. caftaric acid 2. chlorogenic acid 3. echinacoside 4. cichoric acid. phenethyl glucosinolate Inj.: 0µL Conc.: 000µg/mL Solvent: water Column: Ultra Aqueous C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å Temp.: LC_066 3 A: 50mM potassium phosphate, ph 2.5 B: acetonitrile Time (min.): % B mL/min. ambient 20nm Tocopherols in Soy Oil on Pinnacle II Silica. α-tocopherol 2. β-tocopherol 3. γ-tocopherol 4. δ-tocopherol Inj.: 0µL Conc.: approx..25% soy oil Solvent: hexane Column: Pinnacle II Silica Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å isopropyl alcohol:hexane (0.5:99.5) 0.6 ml/min. Temp.: 30 C 295nm HPLC APPLICATIONS NUTRACEUTICAL 4 2 LC_ min. LC_097

80 78 Preservatives, Vitamins Parabens on Pinnacle II C8 Parabens on Pinnacle II Phenyl Column: Pinnacle II C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å Column: Pinnacle II Phenyl Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å HPLC APPLICATIONS FOODS & FLAVORS Vitamins (Fat Soluble) on Ultra C8 Conc.. (mg/ml). solvent front n/a 2. menadione (vitamin K3) all-trans-retinol (vitamin A) vitamin D unknown n/a Solvent: diethyl ether 6. α tocopherol (vitamin E) α tocopherol acetate (vitamin E acetate) 2.4 Column: Cat. #: Ultra C unknown n/a Dimensions: 250 x 4.6mm 9. phylloquinone (vitamin K) 0.84 Pore size: 00Å 2 3 LC_076 4 acetonitrile:methanol (90:0, v/v) Time (min.) Flow (ml/min.) Temp.: 30 C 280nm Conc. (µg/ml). methyl paraben 0 2. ethyl paraben propyl paraben butyl paraben 40 Inj.: 5µL Solvent: methanol:water (50:50 v/v) Temp.: 0.% acetic acid in water: acetonitrile (50:50, v/v).0ml/min. ambient 254nm min. Vitamins Thiamin (Vitamin B) and Ascorbic Acid (Vitamin C) on Ultra IBD 2 LC_077 Conc. (mg/ml). thiamin ascorbic acid 2.5 Inj.: Solvent:.0µL mobile phase Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å Temp.: mM potassium phosphate, ph 2.5.0mL/min. ambient 254nm min. LC_044 LC_0227

81 Preservatives, Flavor Compounds 79 Phenolic Antioxidants on Pinnacle II C8 Sorbic and Benzoic Acids on Pinnacle II Phenyl Conc. (ppm). propyl gallate TBHQ BHA + 3-BHA BHT 93 Black Pepper: Piperine on Pinnacle II Cyano. piperine Inj.: 5µL Conc.: 00µg/mL Solvent: methanol Column: Pinnacle II Cyano Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å Temp.: min. LC_098 water:acetonitrile (50:50 v/v).0ml/min. ambient 345nm 3 Inj.: 0µL Solvent: methanol Column: Pinnacle II C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å 4 A = % acetic acid B = methanol Time B (min.) (%) mL/min. Temp.: 30 C 280nm Column: Pinnacle II Phenyl Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å % acetic acid:methanol (80:20).0 ml/min. Temp.: 25 C 230nm min. LC_099 Capsaicinoids: Heat Level Assay for Habeñero Nuggets on Pinnacle II C8. unknown 2. capsaicin 3. dihydrocapsaicin Inj.: 0µL habañero nugget extract Conc.: 0.8g sample in 200mL Solvent: ethanol Column: Pinnacle II C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å Temp.: A: % acetic acid in water (v/v) B: acetonitrile Time (min.) % B mL/min. ambient 280nm. sorbic acid 2. benzoic acid Inj.: 0µL Conc.: 00ppm each Solvent: methanol 2 HPLC APPLICATIONS FOODS & FLAVORS min. LC_08 LC_060

82 80 Preservatives, Organic Acids Carboxylic Acids (Pyruvic and Lactic) on Ultra Aqueous C8 Carboxylic Acids on Ultra Aqueous C8 HPLC APPLICATIONS FOODS & FLAVORS Conc. (mg/ml). pyruvic acid 5 2. lactic acid 50 Inj.: 5µL Solvent: 50mM potassium phosphate, ph 2.5 Column: Ultra Aqueous C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 50mM potassium phosphate, ph 2.5.0mL/min. Temp.: 26 C 20nm LC_0297 Organic Acids on Allure Organic Acids. malic acid 2. isocitric acid restek innovation! Excellent peak symmetry in 00% aqueous mobile phase! Inj.: 5µL Conc.: approx. 0.2 mg/ml malic acid; 0.02 mg/ml isocitric acid Solvent: water Column: Allure Organic Acids Cat. #: Dimensions: 250 x 4.6mm Pore size: 60Å 00mM KH2PO4, ph mL/min. Temp.: ambient 226nm Conc. (µg/ml):. malonic acid lactic acid acetic acid citric acid succinic acid fumaric acid 0 Inj.: 0µL Solvent: water Column: Ultra Aqueous C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 50mM potassium phosphate, ph 2.5: acetonitrile (99:).5mL/min. Temp.: 25 C 20nm min. LC_040 Organic Acids on Allure Organic Acids restek innovation! Baseline resolution of tartaric and quinic acids in 00% aqueous mobile phase! 3 Conc. (mg/ml). tartaric acid 2. quinic acid 3. malic acid 4. citric acid 5. fumaric acid Inj.: Solvent: 4 0µL standard solution water Column: Allure Organic Acids Cat. #: Dimensions: 300 x 4.6mm Pore size: 60Å 00mM phosphate buffer, ph mL/min. Temp.: ambient 226nm 5 6 LC_03 LC_0238

83 Organic Acids, Sugars, Flavoring 8 Organic Acids in Cranberry Juice on Allure Organic Acids Sugars on Pinnacle II Amino (3μm). quinic acid 2. malic acid 3. citric acid Inj.: 0µL Conc.: cranberry juice:water (50:50, v/v) Solvent: water Column: Allure Organic Acids Cat. #: Dimensions: 300 x 4.6mm Pore size: 60Å 00mM phosphate buffer, ph mL/min. Temp.: ambient 226nm LC_0236 Sugars: Maple Syrup on Pinnacle II Amino. fructose 2. glucose 3. sucrose 3 Inj.: Conc.: Solvent: 50.0µL % maple syrup in solvent acetonitrile:water (70:30, v/v) Column: Pinnacle II Amino Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å water:acetonitrile (25:75).0mL/min. Temp.: 35 C refractive 35 C Conc. (mg/ml). fructose glucose sucrose maltose lactose 4.4 Inj.: 5µL Solvent: mobile phase Column: Pinnacle II Amino Cat. #: Dimensions: 50 x 4.6mm Particle size: 3µm Pore size: 0Å water:acetonitrile (25:75, v/v).5 ml/min. Temp.: 35 C refractive 35 C LC_0223 Vanilla Bean Extract on Pinnacle DB C8 (Fast LC) Inj.: 5µL Conc.: 20% v/v commercial extract in water Column: Pinnacle DB C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 40Å 0.2% v/v phosphoric acid in water:methanol (80:20 v/v) 2mL/min. Temp.: 27 C 254nm Peak List Ret. Time (min.). vanillin 2.00 HPLC APPLICATIONS FOODS & FLAVORS min. LC_0205 LC_0252

84 82 Flavorings, Food Contaminants HPLC APPLICATIONS FOODS & FLAVORS Vanillin on Ultra C8 Inj.: Conc.: Solvent: 0mL 5% solution of vanilla extract 40% ethanol Column: Ultra C8 Cat. #: Dimensions: 50x4.6mm Pore size: 00Å Mobile phases: A:.2% acetic acid in water B: methanol Time (min.) % B mL/min. Temp.: 28 C 254nm. vanillin min. LC_049 Sudan Dyes on Ultra Aqueous C8 Photodiode Array Detector Vanillin and Ethyl Vanillin on Ultra C8 Conc. (mg/ml). vanillin ethyl vanillin 0.04 Inj.: 0µL Solvent: 40% ethanol Column: Ultra C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å Mobile phases: A:.2% acetic acid B: methanol Time (min.) %B mL/min. Temp.: 28 C 254nm min. Fixed Dual Wavelength Detector LC_048 2 new!. Sudan I 2. Sudan II 3. Sudan III 4. Sudan IV wavelength change Monitor Sudan I, II, III, and IV in a single, isocratic analysis! LC_FF0326 LC_FF0327 Inj.: 20µL, mixture of Sudan I, Sudan II, Sudan III, Sudan IV Conc.: 20µg/mL each component Sample diluent: methanol Column: Ultra Aqueous C8 Cat.#: Dimensions: 50 x 4.6 mm Pore size: 00Å methanol:water, 97:3 ml/min. Temp.: ambient 476 / 493 / 52 / 357 nm (left) or 488 / 520 nm (right)

85 Food Contaminants 83 Nitrofuran Metabolites by LC-MS/MS on Ultra C8 d5-amoz AMOZ Conc. (ppb) Time (min). d5-amoz AMOZ AHD d4-aoz AOZ SC Column: Ultra C8 Cat. #: Dimensions: 00 x 2.mm Particle size: 3µm Pore size: 00Å A: 0.05% formic acid in methanol B: 0.05% formic acid 5 mm NH4 formate in water Time (min) %B AHD d4-aoz AOZ LC_ µL/min. Temp.: 30 C MS/MS triple quadrupoles (Thermo Scientific Discovery ) SC Analyzer Parameters: Ion source: ESI (electrospray ionization) Only segment: 5 min. Polarity: positive Data type: centroid Scan mode: SRM product Scan width (m/z): 0.7 Scan time (s): 0.25 Peak width: Q: within 0.7 Q2: 0.7 Collision gas pressure (mtorr):.5 (argon) Divert valve: active, with 3 positions Positions- 2 min., 2 8 min., 3 5 min. Analyte Prec. Ion Prod. Ion Collision E Tube Lens AOZ V 20 AMOZ V 00 SC V 80 AHD V 0 AMOZ = 3-amino-5-morpholinomethyl-2-oxazolidinone AHD = -aminohydantoin hydrochloride AOZ = 3-amino-2-oxazolidinone SC = semicarbazide HPLC APPLICATIONS FOODS & FLAVORS for more info For more information, review Restek Advantage 2005 vol.2, page, on our website. lit. cat.# 59923

86 84 Analgesics Hydroxybenzoic Acids on Ultra Aqueous C8 new!. 3,5-dihydroxybenzoic acid 2. 2,5-dihydroxybenzoic acid 3. 4-hydroxybenzoic acid 4. 3-hydroxybenzoic acid 5. 2,4-dihydroxybenzoic acid 6. benzoic acid 7. salicylic acid HPLC APPLICATIONS PHARMACEUTICAL Analgesic Acetaminophen and Narcotic Analgesics on Allure Basix 2. acetaminophen 2. codeine 3. oxycodone 4. hydroxycodone Inj.: 5µL Conc.: 00µg/mL Solvent: mobile phase Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å LC_PH mM ammonium acetate, ph 4.5: acetonitrile (85:5, v/v) Flow rate:.0ml/min. Temp.: 30 C 280nm Conc. (µg/ml) U. unknown unknown. morphine sulfate acetaminophen codeine phosphate oxycodone HCl hydrocodone bitartrate 28 Inj.: 5µL Conc.: 50µg/mL each component Sample diluent: mobile phase Column: Ultra Aqueous C8 Cat.#: Dimensions: 50 x 4.6mm Pore size: 00Å A: 20mM potassium phosphate (ph 2.5); B: acetonitrile Time (min.) %B mL/min. Temp.: ambient 20nm Analgesic Acetaminophen and Narcotic Analgesics on Ultra C8 Inj.: Solvent: 4.0µL raw material mix mobile phase Column: Ultra C8 Catalog #: Dimensions: 250 x 4.6mm Pore size: 00Å A: 0mm potassium phosphate, ph 2.8 B: acetonitrile:methanol, (90:0, v/v) (85A:5B, v/v).0ml/min. Temp.: 35 C 235nm min. LC_0082 LC_022

87 Analgesics, Antiarrhythmics 85 Narcotic Analgesic Morphine Sulfate Raw Material - USP 25: Resolution Solution on Pinnacle II C8 Conc. (µg/ml). unknown unknown 2. phenol morphine sulfate 0.24 Inj.: Solvent: Column: Pinnacle II C8 Cat. #: Dimensions: 250 x 4.6mm Pore size: 0Å Temp.: 20.0µL mobile phase Column: Ultra C8 Cat. #: Dimensions: 50 x 4.0mm Pore size: 00Å A: 0.73g -heptane sulfonic acid and 0mL glacial acetic acid, diluted to 720mL with water, ph=2.33 B: methanol (72A:28B, v/v).00ml/min, 26.5 C 284nm LC_0209 Narcotic Analgesic Oxycodone HCl Raw Material by USP 25: Resolution Solution on Ultra C8 Conc. (µg/ml) Ret. Time (min.) RRT Tailing Res.. codeine phosphate oxycodone HCl Inj.: 0µL resolution solution Solvent: mobile phase A: 5mM hexane sulfonic acid with 2mL TEA and 3mL of phosphoric acid per liter. ph adjusted to 2.5 B: methanol (90A:0B, v/v).5ml/min. Temp.: 40 C 206nm Narcotic Analgesic Hydrocodone Bitartrate by USP 25 on Pinnacle II Silica Ret. Time (min.) Inj. % RSD (n=5). hydrocodone bitartrate % Inj.: 20µL Conc.: µg/ml assay standard Solvent: mobile phase Column: Pinnacle II Silica Cat. #: Dimensions: 250 x 4.6mm Pore size: 0Å.5mL/min. Temp.: 27 C 280nm Impurities resolved from primary peak. A: 800mL acetonitrile; 4mL water; ml diethylamine B: methanol (55A:45B, v/v) min. LC_02 Antiarrhythmic Calcium Channel Blockers on Ultra Cyano. diltiazem 2. nifedipine impurity 3. verapamil 4. nifedipine 5. nicardipine Inj.: 5µL Conc.: 00mg/mL Solvent: acetonitrile:water (:) Column: Ultra Cyano Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å Flow rate: Temp.: 20mM potassium phosphate monobasic, ph 3.0:acetonitrile (70:30, v/v).2ml/min. 30 C (± C) 235nm 4 5 HPLC APPLICATIONS PHARMACEUTICAL 3 2 LC_ min. LC_0062

88 86 Antiarrhythmics Digitalis Extracts/Derivatives on Ultra PFP Propyl Cartridge (Fast LC) Antiarrhythmic Furosemide on Ultra IBD HPLC APPLICATIONS PHARMACEUTICAL Conc. Ret. Time (min.). digoxigenin 00µg/mL gitoxigenin 00µg/mL digoxin 00µg/mL.0 4. gitoxin ~0µg/mL digitoxin 00µg/mL 2.60 Inj.: 0µL Solvent: water:acetonitrile (80:20 v/v) Column: Ultra PFP Propyl Cartridge Dimensions: 30 x 4.0mm Particle size: 3µm Pore size: 00Å A: water B: acetonitrile Time (min.) %B mL/min. Temp.: 27 C 230nm LC_0250 Antiarrhythmic Atenolol on Allure Basix 2. uracil (marker) 2. atenolol Inj.: µl Conc.: mg/ml Solvent: water:methanol (7:3, v/v) Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å acetonitrile:20mm ammonium acetate, ph 4.5 (80:20, v/v).2ml/min. Flow rate: Temp.: 25 C 225nm. uracil (marker) 2. furosemide Inj.: 5µL Conc.: 000µg/mL Solvent: acetonitrile:water (60:40, v/v) Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 20mM ammonium acetate, ph 4.5: acetonitrile (70:30, v/v) Flow rate: 2.0mL/min. Temp.: 27 C 280nm LC_007 Impurities in Furosemide (enlargement of baseline) Antiarrhythmic Enalapril Maleate on Allure Basix. maleate 2. enalapril Inj.: Conc.: Solvent: 2.5µL.mg/mL methanol:water (30:70) Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 2.5: acetonitrile (20:80, v/v) Flow rate:.2ml/min. Temp.: 25 C 225nm min. LC_0072 LC_009

89 Antiarrhythmics 87 Antiarrhythmic Timolol Maleate on Allure Basix Antiarrhythmic Metoprolol on Allure Basix. maleate 2. timolol Inj.: 2.5µL Conc.:.2mg/mL Solvent: methanol:water (30:70) Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 2.5: acetonitrile (20:80, v/v) Flow rate:.2ml/min. Temp.: 25 C 290nm LC_0092 Antiarrhythmic Triamterene and Hydrochlorothiazide on Allure Basix. hydrochlorothiazide 2. triamterene Inj.: µl Conc.: 500µg/mL Solvent: methanol:water: tetrahydrofuran (30:35:35) Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 20mM ammonium acetate, ph 4.5:acetonitrile (65:35,v/v) Flow rate:.2ml/min. Temp.: 25 C 225nm. unknown 2. metoprolol Inj.: Conc.: Solvent: 2.5µL.5mg/mL methanol:water (30:70) Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 2.5: acetonitrile (0:90, v/v) Flow rate:.2ml/min. Temp.: 25 C 225nm LC_0090 Antiarrhythmic Verapamil HCl on Allure Basix. toluene (marker) 2. verapamil HCl Inj.: µl Conc.: mg/ml Solvent: water:acetonitrile (70:30, v/v) Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å acetonitrile: 20mM ammonium acetate, ph 4.5 (65:35,v/v) Flow rate:.2ml/min. Temp.: 25 C 230nm 2 HPLC APPLICATIONS PHARMACEUTICAL min. LC_0086 LC_0077

90 88 Antiasthmatics, Antibiotics Antiasthmatic Beclomethasone on Ultra C8 Antibiotic Amoxicillin on Ultra IBD HPLC APPLICATIONS PHARMACEUTICAL. beclomethasone 2. deschlorobeclomethasone Inj.: 0µL Conc.: 79.5µg/mL (beclomethasone) Solvent: water:acetonitrile (0:90, v/v) Column: Ultra C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å water:acetonitrile (70:30, v/v).0ml/min. Temp.: 25 C 238nm min. LC_020 Antibiotic Cephalexin on Ultra IBD 2. amoxicillin Inj.: 5µL Conc.:.5mg/mL Solvent: acetonitrile:water (0:90, v/v) Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 0mM ammonium formate, ph 2.5: acetonitrile (95:5, v/v) Flow rate:.2ml/min. Temp.: 30 C 270nm min. LC_0095 Antibiotic Penicillin V on Ultra IBD. cephalexin 2. cephradine Inj.: 0µL Conc.: 500µg/mL Solvent: acetonitrile:water (0:90, v/v) Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 0mM ammonium formate, ph 2.5: acetonitrile (90:0, v/v) Flow rate:.2ml/min. Temp.: 30 C 270nm 2. penicillin V Inj.: Conc.: Solvent: 2.5µL.2mg/mL acetonitrile:water (0:90,v/v) Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 0mM ammonium formate, ph 2.5: acetonitrile (95:5, v/v) Flow rate:.2ml/min. Temp.: 30 C 270nm min min. LC_0094 LC_0096

91 Antibiotics, Antidepressants 89 Antibiotic Sulfamethoxazole on Allure Basix. sulfamethoxazole Inj.: 0µL Conc.: 00µg/mL Solvent: water:methanol (:, v/v) Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å Flow rate: Temp.: water:methanol (70:30, v/v).0ml/min. ambient 254nm min. LC_0066 Antibiotic Cephaloridine on Ultra IBD (Reversed Phase). cephaloridine Inj.: 5µL Conc.: mg/ml Solvent: acetonitrile:water (50:50, v/v) Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å acetonitrile: 20mM ammonium phosphate, ph 4.0 (20:80,v/v).2mL/min. Temp.: 27 C 254nm Antibiotic Sulfamethoxazole, Trimethoprim, and Naphthoate on Allure C trimethoprim 2. sulfamethoxazole 3. naphthoate Inj.: 0µL Conc.: 00µg/mL Solvent: water:methanol (:, v/v) Column: Allure C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å Flow rate: Temp.: 0mM ammonium formate, ph to 3.0 with formic acid: methanol (50:50, v/v).0ml/min. ambient 220nm min. LC_0067 Antidepressants (Tricyclic) on Allure Basix. doxepin 2. nordoxepin 3. amitriptyline 4. imipramine 5. desimipramine 6. nortriptyline 7. protriptyline 2 3 Inj.: 0µL Conc.: 00µg/mL each Solvent: mobile phase Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å acetonitrile: 50mM KH2PO4 ph3:methanol (60:25:5, v/v) Flow rate:.2ml/min. Temp.: 30 C 254nm HPLC APPLICATIONS PHARMACEUTICAL min min. LC_0053 LC_002

92 90 Selective Serotonin Reuptake Inhibitors (SSRIs) HPLC APPLICATIONS PHARMACEUTICAL Tetracycline Mix on Allure Biphenyl Ret. Time Peak List (min.). oxytetracycline degradation peak tetracycline degradation peak.6 5. chlortetracycline doxycycline 9.66 Inj.: 30µL Conc.: 00µg/mL each component Sample diluent: methanol Column: Allure Biphenyl Cat. #: Dimensions: 50 x 4.6 mm Pore size: 60Å Temp.: 20mM ammonium phosphate (ph 2.5):acetonitrile, 80:20 ml/min. ambient 254 nm LC_PH0347 Selective Serotonin Reuptake Inhibitors (SSRIs) on phidelity C8 Oxytetracycline and Tetracycline on Allure Biphenyl Ret. Time Peak List (min.). oxytetracycline degradation peak tetracycline 7.0 Inj.: 20µL Conc.: 00µg/mL each component Sample diluent: methanol Column: Allure Biphenyl Cat. #: Dimensions: 50 x 4.6 mm Pore size: 60Å Temp.: 20mM ammonium phosphate (ph 2.5):acetonitrile, 80:20 ml/min. ambient 254 nm Peak List. uracil (marker) 2. fluvoxamine maleate 3. fluoxetine 4. sertraline HCl Inj.: 0µL Conc.: 00µg/mL each component Sample diluent: acetonitrile Column: phidelity C8 Cat.#: Dimensions: 50 x 4.6 mm Particle size: 3µm Pore size: 200Å LC_PH0340 new! 0mM ammonium bicarbonate (ph ): acetonitrile:tetrahydrofuran, 45:45:0.5mL/min. Temp.: ambient 230 nm LC_PH036

93 Anti-Diabetic, Anti-Ulcerative, Bronchodilator, Corticosteroids 9 Anti-Diabetic Glyburide (Glybenclamide) on Ultra IBD 2 Anti-Ulcerative Cimetidine on Ultra IBD (LC/MS). unknown 2. glyburide Inj.: µl Conc.: 500mg/mL Solvent: water:methanol:acetone (::2, v/v) Column: Ultra IBD Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å water with 0.% (v/v) acetic acid: acetonitrile (95:05, v/v) Flow rate:.0ml/min. Temp.: 27 C 254nm min. LC_0069 Bronchodilator Albuterol on Allure Basix. albuterol Inj.: Conc.: Solvent: 2.5µL.mg/mL methanol:water (30:70) Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 2.5: acetonitrile (20:80, v/v) Flow rate:.2ml/min. Temp.: 25 C 225nm. cimetidine Inj.: 5µL Conc.: 0µg/mL Solvent: mobile phase Column: Ultra IBD Cat. #: Dimensions: 50 x 2.mm Pore size: 00Å water:acetonitrile (20:80, v/v) 0.2 ml/min. Column temp.: ambient PE/Sciex API 50 EX Interface: turbo ion spray Ion mode: positive Ion spray voltage: 4800V Temp.: 350 C Orifice: 0V Ring: 00V Auxiliary gas: 6000cc/min min. LC_05 Corticosteroids on Pinnacle II Cyano (Reversed Phase) Conc. (mg/ml). cortisone corticosterone cortisone acetate 0.7 Inj.: Solvent: 5.0µL methanol Column: Pinnacle II Cyano Cat. #: Dimensions: 50 x 4.6mm Pore size: 0Å water:methanol (65:35, v/v).0ml/min. Temp.: 30 C 254nm Pressure: 33 bar 2 3 HPLC APPLICATIONS PHARMACEUTICAL min. LC_ min. LC_086

94 92 Corticosteroids Corticosteroids on Ultra C8 Corticosteroids on Allure Biphenyl HPLC APPLICATIONS PHARMACEUTICAL. uracil 2. triamcinolone 3. hydrocortisone 4. dexamethasone 5. corticosterone 6. deoxycorticosterone Inj.: 5µL Conc.: 00µg/mL Solvent: mobile phase Column: Ultra C8 Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å water:methanol (30:70, v/v).0ml/min. Temp.: 30 C 220nm min. LC_008 Corticosteroids on Allure Biphenyl Ret. Time Peak List (min.). hydrocortisone prednisone cortisone dexamethasone corticosterone cortisone acetate desoxycorticosterone Inj.: 5µL Conc.: 00µg/mL each component Sample diluent: methanol Column: Allure Biphenyl Cat. #: Dimensions: 50 x 4.6 mm Pore size: 60Å water:acetonitrile, 60:40 ml/min. Temp.: ambient 254 nm LC_PH0330 new! 4 Concentration Peak List (µg/ml). hydrocortisone prednisone cortisone betamethasone dexamethasone 52 Excellent resolution of isomers! Inj.: 0µL Sample diluent: methanol Column: Allure Biphenyl Cat.#: Dimensions: 50 x 4.6 mm Particle size: 3µm Pore size: 60Å Temp.: water:acetonitrile, 70:30 (v/v).5ml/min. ambient 220nm LC_PH0384

95 Steroids 93 Endogenous Steroids on Allure Biphenyl Ret. Time Peak List (min.). β-estradiol testosterone progesterone 4.05 Inj.: 5µL Conc.: 00µg/mL each component Sample diluent: methanol Column: Allure Biphenyl Cat. #: Dimensions: 50 x 4.6 mm Pore size: 60Å water:acetonitrile, 40:60 ml/min. Temp.: ambient 220 nm Steroid Hormones on Allure Biphenyl LC_PH0334 Contraceptive Hormones on Allure Biphenyl Ret. Time Peak List (min.). β-estradiol ethynyl estradiol norethindrone norgestrel 8.4 Inj.: 5µL Conc.: 00µg/mL each component Sample diluent: methanol Column: Allure Biphenyl Cat. #: Dimensions: 50 x 4.6 mm Pore size: 60Å Temp.: water:acetonitrile, 40:60 ml/min. ambient 220 nm LC_PH0328 new! HPLC APPLICATIONS PHARMACEUTICAL 2 3 Resolve steroid hormones in a simple, isocratic analysis. Excellent resolution, including isomers! Peak List. estriol 2. 7β-estradiol 3. 7α-estradiol 4. 7α-ethynyl estradiol 5. testosterone 6. estrone 7. norethindrone Inj.: 0µL Conc.: 50µg/mL each component Sample diluent: acetonitrile:methanol, 4: (v/v) Column: Allure Biphenyl Cat.#: Dimensions: 50 x 4.6 mm Particle size: 3µm Pore size: 60Å LC_BP0385 Temp.: water:acetonitrile, 50:50 (v/v).5ml/min. ambient 220nm

96 94 Steroids, Levothyroxine, Basic Drugs Contraceptive Hormones on Allure Basix Hormone Levothyroxine on Ultra PFP HPLC APPLICATIONS PHARMACEUTICAL. uracil (marker) 2. norethindrone 3. medroxyprogesterone Inj.: 0µL Conc.: 00µg/mL Solvent: mobile phase Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å water:acetonitrile (40:60, v/v).0ml/min. Flow rate: Temp.: 30 C 220nm min. LC_0083 Basic Drugs on phidelity C unknown 2. levothyroxine Inj.: 20µL Conc.:.mg/mL Solvent: water:methanol (70:30) Add 3 drops of concentrated ammonium hydroxide to each 0mL of solvent. Levothyroxine will not dissolve unless the ph is basic. Column: Ultra PFP Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 0mM ammonium formate, ph 2.5: methanol (60:40, v/v) Flow rate:.2ml/min. Temp.: 25 C 254nm min. LC_0093 new! Retention Time Peak List pka (min.). lidocaine verapamil diphenhydramine nortriptyline Inj.: 0µL Conc.: 00µg/mL each component Sample diluent: acetonitrile Column: phidelity C8 Cat.#: Dimensions: 50 x 4.6 mm Particle size: 3µm Pore size: 200Å Temp.: 0mM ammonium bicarbonate (ph ): acetonitrile: tetrahydrofuran, 35:55:0 (v/v/v).5ml/min. ambient 254 nm LC_PH0363

97 Lactulose Concentrate, Xanthines, Sedatives 95 Laxative Lactulose Concentrate by USP 25: Resolution Solution on Pinnacle II Amino Conc. (mg/ml) Ret. Time (min.) RRT Res. Inj. % RSD (n=5). fructose galactose epi-lactose unknown lactulose % 5. lactose Inj.: 20µL resolution standard Solvent: acetonitrile:water (50:50) Column: Pinnacle II Amino Cat. #: Dimensions: 50 x 4.6mm Particle size: 3µm Pore size: 0Å LC_0222 Sedatives Alprazolam and Lorazepam on Allure Basix 0mM sodium phosphate monobasic in water, ph 4.8: acetonitrile (22:78, v/v).3ml/min. Temp.: 40 C refractive 40 C Xanthines on Pinnacle DB C min. Sedative Cyclobenzaprine on Allure Basix 3 Conc. (µg/ml). theobromine theophylline β-hydroxyethyltheophylline caffeine 400 LC_024 4 Inj.: 2µL Solvent: mobile phase Column: Pinnacle DB C8 Cat. #: Dimensions: 50 x 4.6 mm Pore size: 40Å 20mM KH2PO4, ph 3.0: acetonitrile (90:0 v/v).0 ml/min. Temp.: 27 C 254nm 2 HPLC APPLICATIONS PHARMACEUTICAL. toluene (marker) 2. lorazepam 3. alprazolam Inj.: 0µL Conc.: 500µg/mL Solvent: water:acetonitrile (7:3, v/v) Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 3.0:acetonitrile (70:30, v/v) Flow rate: 2.0mL/min. Temp.: 25 C 230nm 2 3. toluene (marker) 2. cyclobenzaprine HCl Inj.: µl Conc.:.5µg/mL Solvent: 00% water Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 3.0 acetonitrile (25:75, v/v) Flow rate:.2ml/min. Temp.: 30 C 230nm min. LC_ min. LC_0087

98 96 Admixed Drugs Admixtures: Analgesic Ibuprofen and Decongestant Pseudoephedrine on Allure Basix Admixtures: Analgesic Acetaminophen, Decongestant Pseudoephedrine and Stimulant Caffeine on Allure Basix HPLC APPLICATIONS PHARMACEUTICAL. uracil 2. ibuprofen 3. pseudoephedrine 2 3 LC_0098 Inj.: 5µL Conc.: 200µg/mL Solvent: mobile phase Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium acetate, ph 6.9: methanol (75:25, v/v).0ml/min. Temp.: 25 C 20nm min. Admixtures: Hydrocodone Bitartrate/Acetaminophen by USP 25 on Ultra C8 Conc. Ret. Time RRT Tailing Resolution Inj. %RSD (µg/ml) (min.) (n=5). acetaminophen hydrocodone bitartrate Inj.: 25µL 500mg acetaminophen with 5mg hydrocodone bitartrate (tablet) Solvent: mobile phase Column: Ultra C8 Cat. #: Dimensions: 250 x 4.6mm Pore size: 00Å Temp.: 27 C 6.8g potassium monobasic with 0.2mL TEA per liter: acetonitrile (85:5, v/v).5ml/min. 20nm (hydrocodone bitartrate) and 295nm (acetaminophen) sample solvent 2. caffeine 3. acetaminophen 4. pseudoephedrine Inj.: 5µL Conc.: 00µg/mL Solvent: mobile phase Column: Allure Basix Cat. #: Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium acetate, ph 6.9: methanol (75:25, v/v).0ml/min. Temp.: 25 C 20nm min. LC_0097 Admixtures: Expectorant/Antitussive Guaifenesin and Narcotic Analgesic Codeine on Ultra Phenyl Conc. (µg/ml). codeine guaifenesin 375 Inj.: 5µL Solvent: methanol:water (50:50) Column: Ultra Phenyl Cat. #: Dimensions: 50 x 4.6mm Pore size: 00Å 0mM ammonium formate, ph 2.5: acetonitrile (80:20, v/v) Flow rate:.2ml/min. Temp.: 25 C 280nm min. LC_022 LC_0088

99 HPLC APPLICATIONS 97 acenaphthene acenaphthylene acetaldehyde , 69 acetaminophen , 96 acetic acid acetone , 69 acifluorfen acrolein , 69 adenine adenosine ADP AHD (-aminohydantoin hydrochloride) albuterol aldicarb aldicarb sulfone aldicarb sulfoxide allicin alprazolam amino-4,6-dinitrotoluene , 70 4-amino-2,6-dinitrotoluene , 70 amitriptyline amoxicillin AMOZ (3-amino-5-morpholinomethyl- 2-oxazolidinone) d5-amoz (3-amino-5-morpholinomethyl- 2-oxazolidinone) AMP angiotensin I angiotensin II, III anthracene AOZ (3-amino-2-oxazolidinone) d4-aoz (3-amino-2-oxazolidinone) apomyoglobin ascorbic acid atenolol ATP atrazine beclomethasone bentazon benzaldehyde , 69 benzene benzo(a)anthracene benzo(b)fluoranthene benzo(k)fluoranthene benzo(ghi)perylene benzo(a)pyrene benzoic acid , 84 betamethasone BHA BHA BHA BHT biphenyl butyl paraben butyraldehyde , 69 caffeine , 96 caftaric acid capsaicin carbaryl carbazole carbofuran CDP cephalexin cephaloridine cephradine chloramben chlorogenic acid chlortetracycline chlortoluron , 74 chrysene cichoric acid cimetidine citric acid , 8 CMP COC cocaine codeine , 96 codeine phosphate , 85 corticosterone , 92 cortisone , 92 cortisone acetate , 92 crotonaldehyde , 69 cyanazine , 74 cyclobenzaprine HCl cytidine cytochrome c cytosine , 67 2,4-D , 72 2,4-DB deschlorobeclomethasone desimipramine desisopropylatrazine desoxycorticosterone dexamethasone dibenzo(a,h)anthracene dicamba ,4-dichlorobenzene , 72 3,5-dichlorobenzoic acid ,4-dichlorophenylacetic acid dichlorprop diflubenzuron digitoxin digoxigenin digoxin dihydrocapsaicin ,4-dihydroxybenzoic acid ,5-dihydroxybenzoic acid ,5-dihydroxybenzoic acid diltiazem ,7-dimethylxanthine ,2-dinitrobenzene , 70,3-dinitrobenzene , 70 3,4-dinitrobenzene ,4-dinitrotoluene , 70 2,6-dinitrotoluene , 70 3,4-dinitrotoluene , 70 dinoseb diphenhydramine diquat , 75 diuron , 73, 74 dopamine doxepin doxycycline ecgonine methyl ester echinacoside EME enalapril epi-lactose epinephrine α-estradiol β-estradiol β-estradiol estriol estrone ethofumesate , 74 ethyl paraben ethyl vanillin ethyluracil ethynyl estradiol α-ethynyl estradiol fluometuron fluoranthene fluorene fluoxetine flusilazole , 74 fluvoxamine maleate formaldehyde , 69 fructose , 95 fumaric acid furosemide galactose gitoxigenin gitoxin glucose glyburide guaifenesin guanine guanosine hexanaldehyde , 69 HMX , 70 holo-transferrin hydrochlorothiazide hydrocodone bitartrate , 85, 96 hydrocortisone hydroxybenzoic acid hydroxybenzoic acid hydroxycarbofuran hydroxycodone β-hydroxyethyltheophylline hypoxanthine ibuprofen imipramine indeno(,2,3-cd)pyrene isocitric acid isoproturon , 74 lactic acid lactose , 95 lactulose leucine enkephalin levodopa levothyroxine lidocaine linuron , 73, 74 lorazepam maleate , 87 malic acid , 8 malonic acid maltose MCPA MCPP medroxyprogesterone MEK menadione meth enkephalin methabenzthiazuron , 74 methacrolein , 69 methiocarb methomyl methyl ethyl ketone , 69 methyl paraben methylcytosine methylpurine metoprolol monuron morphine sulfate , 85 N,N'-bis-(chlorobenzyl)urea naphthalene , 76 naphthoate neburon , 74 nicardipine nifedipine nitrobenzene , 70 4-nitrophenol nitrotoluene , 70 3-nitrotoluene , 70 4-nitrotoluene , 70 nordihydrocapsaicin nordoxepin norepinephrine norethindrone , 94 norgestrel nortriptyline , 94 oxamyl oxycodone oxycodone HCl , 85 oxytetracycline paraquat , 75 pendimethalin penicillin V pentachlorophenol phenanthrene phenethyl glucosinolate phenol phenylalanine phylloquinone picloram piperine prednisone progesterone propanil propionaldehyde , 69 propoxur propyl gallate propyl paraben propyluracil protriptyline pseudoephedrine purine pyrene pyruvic acid quinic acid , 8 RDX , 70 all-trans-retinol ribonuclease A salicylic acid SC (semicarbazide) secbumeton , 74 sertraline HCl siduron Silvex , 72 simazine sorbic acid succinic acid sucrose Sudan I Sudan II Sudan III Sudan IV sulfamethoxazole ,4,5-T tartaric acid TBHQ TDP tebuthiuron terbumeton terbuthylazine terbuthylazine desethyl testosterone tetracycline Tetryl , 70 theobromine , 95 theophylline , 95 thiamin thidiazuron thymidine thymine , 67 timolol TMP α-tocopherol , 78 α-tocopherol acetate β-tocopherol δ-tocopherol γ-tocopherol m-tolualdehyde , 69 toluene ,4,5-TP (Silvex) triamcinolone triamterene trimethoprim ,3,5-trinitrobenzene , 70 2,4,6-trinitrotoluene , 70 tryptophan TTP tyrosine , 68 UDP UMP uracil , 66, 67, 86, 90, 92, 94 uric acid uridine UTP val-4-angiotensin III val-tyr val-tyr-val valeraldehyde , 69 vanillin , 82 verapamil , 94 verapamil HCl vitamin D xanthine Compound Index Restek Trademarks: Allure, phidelity, Pinnacle, Restek logo, Sidewinder, Trident. Other Trademarks: Kel-F (3M Co.), Zorbax (Agilent), API 3200 (Applied Biosystems), FlatLine, HyperShear, QuickSplit (Analytical Scientific Instruments), Kromasil (Eka Chemicals AB), Teflon, Tefzel, Vespel (E.I. du Pont de Nemours & Co., Inc.), Inertsil (GL Sciences), Snap Seal, Step, Top Hat (J.G. Finneran Associates, Inc.), Clean-Cut, Opti-Cap (Jour Research), LiChrospher (Merck KgaA), Parker (Parker Instrumentation Division), AQUA, Develosil, Luna, Maxsil, Ultracarb (Phenomenex, Inc.), Rheodyne (Rheodyne LP), Tygon (Saint-Gobain Performance Plastics Corp.), LO-Pulse, MicroPulse (Scientific Systems, Inc.), Super-Clean (SGT Middleburg BV), Betamax, Discovery, Hypersil, Prism (Thermo Fisher Scientific), BalanceBank, BenchBooster, LCLocker, phperch, TopLoader (TrippNT, Inc.), Upchurch (Upchurch Scientific), Valco (Valco Instruments Co., Inc.), PEEK (Victrex plc), Alliance, PerformancePLUS, Spherisorb, Super Seals, Symmetry, SymmetryShield, WISP, YMC (Waters Associates, Inc.)

100 Lit. Cat.# C 2007 Restek Corporation. Restek U.S. 0 Benner Circle Bellefonte, PA fax: Restek France phone: 33 (0) fax: 33 (0) restek@restekfrance.fr Restek Ireland phone: fax: restekeurope@aol.com Thames Restek U.K. LTD phone: fax: sales@thamesrestek.co.uk Restek GmBH phone: +49 (0) fax: +49 (0) info@restekgmbh.de