Opportunity and Challenge: the new era of cryo-em. Ning Gao School of Life Sciences Tsinghua University
|
|
|
- Alannah Gray
- 5 years ago
- Views:
Transcription
1 Opportunity and Challenge: the new era of cryo-em Ning Gao School of Life Sciences Tsinghua University
2 Education and Research Experience Principal Investigator present Postdoc, Department of Biochemistry and Biophysics, and HHMI (Advisor: Joachim Frank) Ph.D., Biomedical Sciences (Advisor: Joachim Frank) B.S. Biochemistry and Molecular Biology
3 Research Interest Ribosome biogenesis In vivo assembly pathways and quality control of ribosome maturation Molecular roles of biogenesis factors Translation regulation Ribosomes from human pathogens Understanding Pathogen-specific translation mechanisms Screening for small molecules with preventive and therapeutic potentials. Large RNA/DNA-protein complexes DNA replication initiation Mechano-sensitive channels
4 Representative Structural Snapshots Ribosome MCM2-7 Piezo1
5 Near atomic structure of a ribosomal complex Resolution 4.5 Å From 300,000 particles, CCD data
6 Nature Methods 11, 30 (2014)
7 CCD vs EDD CCD Electron Direct Detector From Gatan website
8 Counting mode and Super-resolution From Gatan website
9 Movie Mode and Motion Correction
10 Bronze Age Iron Age Better detective quantum efficiency (signal-to-noise ratio) Faster speed High Sensitivity Kühlbrandt, 2014, Science
11 Unpublished data
12 Small membrane channel Unpublished data
13
14 800 micrographs and 180,000 particles in 17 hours
15 70S ribosome (3.0 Å) Unpublished data
16 Opportunities Challenges
17 YnaI : a small-conductance mechanosensitive channel (MscS) Yoo et al., Biophys, 2009 Sense mechanical deformation to regulate osmotic pressure. A heptamer with ~ 250 kda in size.
18 Negative staining of YnaI Aggregate in 1 hr Preferred orientation
19 Elusive Side-views
20 1.2/1.3 grid no carbon coated 350 micrographs (K2 camera) 130,000 particles >93% top views
21 1500 micrographs 600,000 particles
22 Atomic model of YnaI (3.6 Å) Trans-membrane a b region c Cytoplasmic region Atomic model + Density map Atomic Model Unpublished data
23 Sample preparation (traditional) Liquid ethane 3-5 l -172 C Liquid N C Ethane: (boiling point), -172 (melting point), cooling speed very fast
24 Traditional EM grids Holey carbon grid Standardized cryo-sample preparation Smaller sample volume Better particle distribution Reproducibility and manipulability Microfluidics + fast screening by LM
25 Piezo: mechano-transduction in eukaryotes Coste et al., 2010, Science
26 Putative topology of Piezo1 Nilius, 2010, EMBO Reports
27 Negative Staining of mpiezo1 2D class averages
28 Cryo-EM of mpiezo1 3 Å FFT 100 nm
29 Mapping the extracellular CAP domain Nilius, 2010, EMBO Reports
30 3D structure of Piezo1 channel CAP Top Bottom Side
31 Sub-region Refinement
32 Fitting of crystal structure of the CAP
33 Flexible Refinement Original Particles Modified Particles
34 TM-helices resolved by FlexRef Original Map Map from modified particles
35 Transmembrane Wings
36 Pore Module of mpiezo1 PH PH PH PH PH1 Anchor OH CED IH CTD
37 Gating Mechanism of mpiezo1 Out Blade CED PH OH IH In Anchor CTD Beam Ge et al., 2015, Nature, accepted
38 A major challenge: heterogeneous data sorting Defined number of conformations Highly dynamic system Multiple (independent) moving parts Divide, conquer, and stitch
39 Projection Theorem p( x, y) z 2 o( x, y, z) dz o(x,y,z) : 3D density distribution of the object z 1 From Frank, Annu. Rev. Biophys. Biomol. Struct. 31:303-19
40 Mathematical Description of molecular flexibility Multiple conformers of a molecular structure can be expressed by modulation of the original density map by deformation functions, D1, D2, D3 Dn, which normally can be expressed by a rotation matrix and a pair of translational parameters (x, y) describing the conformational changes of individual rigid (relatively) domains. During refinement for each particle, in addition to orientation parameters (x, y,,, ), parameters of Dn could also be estimated.
41 Collaborators: THU: Jianlin Lei Maojun Yang Bailong Xiao Jijie Chai Bik Tye (HKUST & Cornell) Suparna Sanyal (Uppsala) John Woolford (CMU) Hyouta Himeno (Hirosaki) Yan Qin (IBP) Acknowledgments Ministry of Science and Technology, China National Natural Sciences Foundation of China Ministry of Education, China EM Facility Setup and Maintainance: Senfang Sui, Hongwei Wang, Xueming Li Jianlin Lei, Yanji Xu
42 Thank you!
43 Structural Snapshots of Actively Translating Human Ribosomes Behrmann et al., 2015, Cell
44 Behrmann et al., 2015, Cell
Application examples of single particle 3D reconstruction. Ning Gao Tsinghua University
Application examples of single particle 3D reconstruction Ning Gao Tsinghua University ninggao@tsinghua.edu.cn Electron Microscopes First electron microscope constructed by Ernst Ruska in 1930 s (1986
Introduction to Three Dimensional Structure Determination of Macromolecules by Cryo-Electron Microscopy
Introduction to Three Dimensional Structure Determination of Macromolecules by Cryo-Electron Microscopy Amit Singer Princeton University, Department of Mathematics and PACM July 23, 2014 Amit Singer (Princeton
History of 3D Electron Microscopy and Helical Reconstruction
T H E U N I V E R S I T Y of T E X A S S C H O O L O F H E A L T H I N F O R M A T I O N S C I E N C E S A T H O U S T O N History of 3D Electron Microscopy and Helical Reconstruction For students of HI
Lecture CIMST Winter School. 1. What can you see by TEM?
Lecture CIMST Winter School Cryo-electron microscopy and tomography of biological macromolecules 20.1.2011 9:00-9:45 in Y03G91 Dr. Takashi Ishikawa OFLB/005 Tel: 056 310 4217 e-mail: takashi.ishikawa@psi.ch
Electron microscopy in molecular cell biology II
Electron microscopy in molecular cell biology II Cryo-EM and image processing Werner Kühlbrandt Max Planck Institute of Biophysics Sample preparation for cryo-em Preparation laboratory Specimen preparation
ParM filament images were extracted and from the electron micrographs and
Supplemental methods Outline of the EM reconstruction: ParM filament images were extracted and from the electron micrographs and straightened. The digitized images were corrected for the phase of the Contrast
Advanced Higher Biology. Unit 1- Cells and Proteins 2c) Membrane Proteins
Advanced Higher Biology Unit 1- Cells and Proteins 2c) Membrane Proteins Membrane Structure Phospholipid bilayer Transmembrane protein Integral protein Movement of Molecules Across Membranes Phospholipid
MODULE 2 : FOUNDATIONS IN BIOLOGY
OCR A LEVEL BIOLOGY MODULE 2 : FOUNDATIONS IN BIOLOGY REVISION NOTES For 2015 onwards specification Miss T Banda All living things are primarily made from 4 key elements: Carbon (C) Hydrogen (H) Oxygen
CIMST Summer School Cryo-electron microscopy for structural biology 11 September, 2014 Dr. Takashi Ishikawa
CIMST Summer School Cryo-electron microscopy for structural biology 11 September, 2014 Dr. Takashi Ishikawa Tel: 056 310 4217 e-mail: takashi.ishikawa@psi.ch Lab webpage: http://www.psi.ch/lbr/takashi-ishikawa
Part 1 X-ray Crystallography
Part 1 X-ray Crystallography What happens to electron when it is hit by x-rays? 1. The electron starts vibrating with the same frequency as the x-ray beam 2. As a result, secondary beams will be scattered
What can electron microscopy tell us about chaperoned protein folding? Helen R Saibil
Review R45 What can electron microscopy tell us about chaperoned protein folding? Helen R Saibil Chaperonins form large, cage-like structures that act as protein folding machines. Recent developments in
sin" =1.22 # D "l =1.22 f# D I: In introduction to molecular electron microscopy - Imaging macromolecular assemblies
I: In introduction to molecular electron microscopy - Imaging macromolecular assemblies Yifan Cheng Department of Biochemistry & Biophysics office: GH-S472D; email: ycheng@ucsf.edu 2/20/2015 - Introduction
Processing Heterogeneity
New Challenges for Processing Heterogeneity Nikolaus Grigorieff Larson, The Far Side Heterogeneity and Biology Translocation, Brilot et al 2013 Glutamate receptor, Dürr et al 2014 Spliceosome, Wahl et
Three-Dimensional Electron Microscopy of Macromolecular Assemblies
Three-Dimensional Electron Microscopy of Macromolecular Assemblies Joachim Frank Wadsworth Center for Laboratories and Research State of New York Department of Health The Governor Nelson A. Rockefeller
Table of contents. Saturday 24 June Sunday 25 June Monday 26 June
Table of contents Saturday 24 June 2017... 1 Sunday 25 June 2017... 3 Monday 26 June 2017... 5 i Saturday 24 June 2017 Quantitative Biology 2017: Computational and Single-Molecule Biophysics Saturday 24
SUPPLEMENTARY INFORMATION
doi:10.1038/nature11991 Supplementary Figure 1 - Refinement strategy for PIC intermediate assemblies by negative stain EM. The cryo-negative stain structure of free Pol II 1 (a) was used as initial reference
Protein Sorting, Intracellular Trafficking, and Vesicular Transport
Protein Sorting, Intracellular Trafficking, and Vesicular Transport Noemi Polgar, Ph.D. Department of Anatomy, Biochemistry and Physiology Email: polgar@hawaii.edu Phone: 692-1422 Outline Part 1- Trafficking
Building a Homology Model of the Transmembrane Domain of the Human Glycine α-1 Receptor
Building a Homology Model of the Transmembrane Domain of the Human Glycine α-1 Receptor Presented by Stephanie Lee Research Mentor: Dr. Rob Coalson Glycine Alpha 1 Receptor (GlyRa1) Member of the superfamily
Molecular electron microscopy
Molecular electron microscopy - Imaging macromolecular assemblies Yifan Cheng Department of Biochemistry & Biophysics office: GH-S427B; email: ycheng@ucsf.edu 2/22/2013 - Introduction of Molecular Microscopy:
PCA from noisy linearly reduced measurements
PCA from noisy linearly reduced measurements Amit Singer Princeton University Department of Mathematics and Program in Applied and Computational Mathematics September 27, 2016 Amit Singer (Princeton University)
The identification of the ph sensor in E. coli MscS
1 Dean s Leadership Council Faculty Fellowship Award Application Hannah R. Malcolm, Assistant Professor of Chemistry Email: hannah.malcolm@unf.edu; Phone: 904-620-1963 The identification of the ph sensor
The Tecnai Arctica (TEM-9) Cryo-EM workflow. Contact: Svetla Stoilova-McPhie, PhD Advanced bioimaging scientist
The Tecnai Arctica (TEM-9) Cryo-EM workflow Contact: Svetla Stoilova-McPhie, PhD Advanced bioimaging scientist stoilovamcphie@fas.harvard.edu The Arctica Cryo-EM training chart Cryo-TEM training involves
REVIEW 2: CELLS & CELL COMMUNICATION. A. Top 10 If you learned anything from this unit, you should have learned:
Name AP Biology REVIEW 2: CELLS & CELL COMMUNICATION A. Top 10 If you learned anything from this unit, you should have learned: 1. Prokaryotes vs. eukaryotes No internal membranes vs. membrane-bound organelles
School of Nuclear Science and Engineering, East China University of Technology,
Large-pore Layered Networks, Polycatenated Frameworks and Three-dimensional Frameworks of Uranyl Tri(biphenyl)amine/Tri(phenyl)amine Tricarboxylate: Solvent/Ligand-dependent Dual Regulation Shuai Wang,,,#
BMB November 17, Single Molecule Biophysics (I)
BMB 178 2017 November 17, 2017 14. Single Molecule Biophysics (I) Goals 1. Understand the information SM experiments can provide 2. Be acquainted with different SM approaches 3. Learn to interpret SM results
A SURPRISING CLARIFICATION OF THE MECHANISM OF ION-CHANNEL VOLTAGE- GATING
A SURPRISING CLARIFICATION OF THE MECHANISM OF ION-CHANNEL VOLTAGE- GATING AR. PL. Ashok Palaniappan * An intense controversy has surrounded the mechanism of voltage-gating in ion channels. We interpreted
Multiple Choice Review- Eukaryotic Gene Expression
Multiple Choice Review- Eukaryotic Gene Expression 1. Which of the following is the Central Dogma of cell biology? a. DNA Nucleic Acid Protein Amino Acid b. Prokaryote Bacteria - Eukaryote c. Atom Molecule
6 Mechanotransduction
6.1 Motivation The process of converting physical forces into biochemical signals and integrating these signals into the cellular response is referred to as mechnotransduction [11, 20]. To fully understand
Chapter 4 Cell Structure and Function Sections 1-6
Chapter 4 Cell Structure and Function Sections 1-6 4.1 Food For Thought E. coli O157:H7A, strain of bacteria that causes severe illness or death, occasionally contaminates foods such as ground beef and
Macromolecular Crowding
Macromolecular Crowding Keng-Hwee Chiam Mathematical and Theoretical Biology Group Goodsell (1994) Macromolecular Crowding, Oct. 15, 2003 p.1/33 Outline What: introduction, definition Why: implications
Chapter 21 PROKARYOTES AND VIRUSES
Chapter 21 PROKARYOTES AND VIRUSES Bozeman Video classification of life http://www.youtube.com/watch?v=tyl_8gv 7RiE Impacts, Issues: West Nile Virus Takes Off Alexander the Great, 336 B.C., conquered a
Homology models of the tetramerization domain of six eukaryotic voltage-gated potassium channels Kv1.1-Kv1.6
Homology models of the tetramerization domain of six eukaryotic voltage-gated potassium channels Kv1.1-Kv1.6 Hsuan-Liang Liu* and Chin-Wen Chen Department of Chemical Engineering and Graduate Institute
Cells Under the Microscope Measuring Cell Structures
Copy into Note Packet and Return to Teacher Chapter 3 Cell Structure Section 1: Looking at Cells Objectives Describe how scientists measure the length of objects. Relate magnification and resolution in
3D Structure Determination using Cryo-Electron Microscopy Computational Challenges
3D Structure Determination using Cryo-Electron Microscopy Computational Challenges Amit Singer Princeton University Department of Mathematics and Program in Applied and Computational Mathematics July 28,
Cellular Systems Biology or Biological Network Analysis
Cellular Systems Biology or Biological Network Analysis Joel S. Bader Department of Biomedical Engineering Johns Hopkins University (c) 2012 December 4, 2012 1 Preface Cells are systems. Standard engineering
CRYSTALLOGRAPHY AND STORYTELLING WITH DATA. President, Association of Women in Science, Bethesda Chapter STEM Consultant
CRYSTALLOGRAPHY AND STORYTELLING WITH DATA President, Association of Women in Science, Bethesda Chapter STEM Consultant MY STORY Passion for Science BS Biology Major MS Biotechnology & Project in Bioinformatics
!"#$%&'%()*%+*,,%-&,./*%01%02%/*/3452*%3&.26%&4752*,,*1%%
!"#$%&'%()*%+*,,%-&,./*%01%02%/*/3452*%3&.26%&4752*,,*1%% !"#$%&'(")*++*%,*'-&'./%/,*#01#%-2)#3&)/% 4'(")*++*% % %5"0)%-2)#3&) %%% %67'2#72'*%%%%%%%%%%%%%%%%%%%%%%%4'(")0/./% % 8$+&'&,+"/7 % %,$&7&/9)7$*/0/%%%%%%%%%%
Nucleus. The nucleus is a membrane bound organelle that store, protect and express most of the genetic information(dna) found in the cell.
Nucleus The nucleus is a membrane bound organelle that store, protect and express most of the genetic information(dna) found in the cell. Since regulation of gene expression takes place in the nucleus,
Biology 112 Practice Midterm Questions
Biology 112 Practice Midterm Questions 1. Identify which statement is true or false I. Bacterial cell walls prevent osmotic lysis II. All bacterial cell walls contain an LPS layer III. In a Gram stain,
GCD3033:Cell Biology. Transcription
Transcription Transcription: DNA to RNA A) production of complementary strand of DNA B) RNA types C) transcription start/stop signals D) Initiation of eukaryotic gene expression E) transcription factors
Regulation and signaling. Overview. Control of gene expression. Cells need to regulate the amounts of different proteins they express, depending on
Regulation and signaling Overview Cells need to regulate the amounts of different proteins they express, depending on cell development (skin vs liver cell) cell stage environmental conditions (food, temperature,
Hands-on Research Complex Systems School SJTU, Shanghai, China (June 17-29, 2012) Biophysics. Eva-Maria Schötz
Hands-on Research Complex Systems School SJTU, Shanghai, China (June 17-29, 2012) Biophysics Eva-Maria Schötz Lewis-Sigler Institute for Integrative Genomics Princeton University What is biophysics? The
Name: TF: Section Time: LS1a ICE 5. Practice ICE Version B
Name: TF: Section Time: LS1a ICE 5 Practice ICE Version B 1. (8 points) In addition to ion channels, certain small molecules can modulate membrane potential. a. (4 points) DNP ( 2,4-dinitrophenol ), as
Chapter 7.2. Cell Structure
Chapter 7.2 Cell Structure Daily Objectives Describe the structure and function of the cell nucleus. Describe the function and structure of membrane bound organelles found within the cell. Describe the
Tutorial 1 Geometry, Topology, and Biology Patrice Koehl and Joel Hass
Tutorial 1 Geometry, Topology, and Biology Patrice Koehl and Joel Hass University of California, Davis, USA http://www.cs.ucdavis.edu/~koehl/ims2017/ Biology = Multiscale. 10 6 m 10 3 m m mm µm nm Å ps
Scale in the biological world
Scale in the biological world 2 A cell seen by TEM 3 4 From living cells to atoms 5 Compartmentalisation in the cell: internal membranes and the cytosol 6 The Origin of mitochondria: The endosymbion hypothesis
Cellular Biology. Cells: theory, types, form & function, evolution
Cellular Biology Cells: theory, types, form & function, evolution The Cell Theory Problems with the Cell Theory? The cell theory has three components: 1. all living organisms are made up of one or more
Purification, SDS-PAGE and cryo-em characterization of the MCM hexamer and Cdt1 MCM heptamer samples.
Supplementary Figure 1 Purification, SDS-PAGE and cryo-em characterization of the MCM hexamer and Cdt1 MCM heptamer samples. (a-b) SDS-PAGE analysis of the hexamer and heptamer samples. The eluted hexamer
Supporting Information
Supporting Information Electrochemiluminescent Pb 2+ -Driven Circular Etching Sensor Coupled to a DNA Micronet-Carrier Wen-Bin Liang,, Ying Zhuo, Ying-Ning Zheng, Cheng-Yi Xiong, Ya-Qin Chai, *, and Ruo
Biochemistry: A Review and Introduction
Biochemistry: A Review and Introduction CHAPTER 1 Chem 40/ Chem 35/ Fundamentals of 1 Outline: I. Essence of Biochemistry II. Essential Elements for Living Systems III. Classes of Organic Compounds IV.
Principles of Cellular Biology
Principles of Cellular Biology آشنایی با مبانی اولیه سلول Biologists are interested in objects ranging in size from small molecules to the tallest trees: Cell Basic building blocks of life Understanding
BIO.A.1 Basic Biological Principles
BIO.A.1 Basic Biological Principles Bio.A.1.1 - Explain the characteristics common to all organisms Bio.A.1.2 Describe relationships between the structure & function at biological levels of organization
Structural Studies of the Flexible Filamentous Plant Viruses
Structural Studies of the Flexible Filamentous Plant Viruses Caitlin Azzo Dr. Gerald Stubbs BSCI 296 [4] Honors Thesis April 23, 2014 The Plant Viruses of Interest In the Potyviridae family: Wheat streak
Model building and validation for cryo-em maps at low resolution
International Workshop of Advanced Image Processing of Cryo-Electron Microscopy 2015 Tsinghua University, Beijing, China June 3-7, 2015 Model building and validation for cryo-em maps at low resolution
The Potassium Ion Channel: Rahmat Muhammad
The Potassium Ion Channel: 1952-1998 1998 Rahmat Muhammad Ions: Cell volume regulation Electrical impulse formation (e.g. sodium, potassium) Lipid membrane: the dielectric barrier Pro: compartmentalization
GENES AND CHROMOSOMES III. Lecture 5. Biology Department Concordia University. Dr. S. Azam BIOL 266/
GENES AND CHROMOSOMES III Lecture 5 BIOL 266/4 2014-15 Dr. S. Azam Biology Department Concordia University CELL NUCLEUS AND THE CONTROL OF GENE EXPRESSION OPERONS Introduction All cells in a multi-cellular
WHAT DO CELLS DO? CHALLENGE QUESTION. What are the functions of the structures inside of cells?
WHAT DO CELLS DO? CHALLENGE QUESTION What are the functions of the structures inside of cells? WHAT DO CELLS DO? Understanding normal cell structures and their functions help scientists understand how
Flow of Genetic Information
presents Flow of Genetic Information A Montagud E Navarro P Fernández de Córdoba JF Urchueguía Elements Nucleic acid DNA RNA building block structure & organization genome building block types Amino acid
Sample Preparation. Holger Stark MPI for biophysical Chemistry Göttingen Germany
Sample Preparation Holger Stark MPI for biophysical Chemistry Göttingen Germany Motivation PDB search results: s with min 4 different chains (complexes) make up only ~1% of the PDB s in contrast to the
Electron Microscopy (TEM and SEM)
7 Electron Microscopy (TEM and SEM) Paul Verkade Wolfson Bioimaging Facility, Physiology & Pharmacology and Biochemistry, University of Bristol, UK 7.1 Basic how-to-do and why-do section 7.1.1 Electron
Problem Set No. 4 Due: Monday, 11/18/10 at the start of class
Department of Chemical Engineering ChE 170 University of California, Santa Barbara Fall 2010 Problem Set No. 4 Due: Monday, 11/18/10 at the start of class Objective: To understand the thermodynamic and
Translation Part 2 of Protein Synthesis
Translation Part 2 of Protein Synthesis IN: How is transcription like making a jello mold? (be specific) What process does this diagram represent? A. Mutation B. Replication C.Transcription D.Translation
Name: Class: Date: ID: A
Class: Date: Ch 7 Review Multiple Choice Identify the letter of the choice that best completes the statement or answers the question. 1. Researchers use fluorescent labels and light microscopy to a. follow
Single Particle Reconstruction with EMAN. GroEL. GroEL 2000 (15 Å) GroEL 2001 (11.5 Å) GroEL 2003 (6 Å)
Single Reconstruction with EMAN Methods for High Resolution Refinement in Single Processing Steve Ludtke Asst. Professor, Biochemistry, BCM Co-director,NCMI Donghua Chen Joanita Jakana Wah Chiu GroEL Jiu-Li
BME 5742 Biosystems Modeling and Control
BME 5742 Biosystems Modeling and Control Lecture 24 Unregulated Gene Expression Model Dr. Zvi Roth (FAU) 1 The genetic material inside a cell, encoded in its DNA, governs the response of a cell to various
Molecular Mechanisms of self-assembly and motion of the bacterial flagellum
Molecular Mechanisms of self-assembly and motion of the bacterial flagellum Mexico-Japan Workshop on Pharmacobiology and Nanobiology Universidad Nacional Autonoma de México (UNAM) Mexico City, Mexico 2009.02.25-26
The Discovery of Cells
The Discovery of Cells Microscope observations! General Cell & Organelle Discovery 1600s Observations made by scientists using more powerful microscopes in the 1800s led to the formation of the cell theory.
2.1 CELL STRUCTURE. The cell is the smallest unit of living organisms that shows the characteristics of life.
2.1.1 Microscopy The cell is the smallest unit of living organisms that shows the characteristics of life. A general introduction to the microscope. The light microscope All cells are microscopic which
UNIT 3 CP BIOLOGY: Cell Structure
UNIT 3 CP BIOLOGY: Cell Structure Page CP: CHAPTER 3, Sections 1-3; HN: CHAPTER 7, Sections 1-2 Standard B-2: The student will demonstrate an understanding of the structure and function of cells and their
Cells vary in size and shape. What limits how large and small cells can be?
10.10.17 What limits how large and small cells can be? Cells vary in size and shape Minimum is determined by the total size of all the molecules required for cellular activity Maximum is limited by the
Molecular Dynamics Flexible Fitting
Molecular Dynamics Flexible Fitting Ryan McGreevy Research Programmer University of Illinois at Urbana-Champaign NIH Resource for Macromolecular Modeling and Bioinformatics Molecular Dynamics Flexible
Recent Developments in Direct Electron Detectors for Electron Cryo-Microscopy
Recent Developments in for Electron Cryo-Microscopy A.R.Faruqi*, R.Henderson and G.McMullan MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH E-mail:
Chapter 6: A Tour of the Cell
AP Biology Reading Guide Fred and Theresa Holtzclaw Chapter 6: A Tour of the Cell Name Period Chapter 6: A Tour of the Cell Concept 6.1 To study cells, biologists use microscopes and the tools of biochemistry
Overview & Applications. T. Lezon Hands-on Workshop in Computational Biophysics Pittsburgh Supercomputing Center 04 June, 2015
Overview & Applications T. Lezon Hands-on Workshop in Computational Biophysics Pittsburgh Supercomputing Center 4 June, 215 Simulations still take time Bakan et al. Bioinformatics 211. Coarse-grained Elastic
7-1 Life Is Cellular. Copyright Pearson Prentice Hall
7-1 Life Is Cellular The Discovery of the Cell What is the cell theory? The Discovery of the Cell The cell theory states: All living things are composed of cells. Cells are the basic units of structure
Curriculum Vitae. Lin Li
Curriculum Vitae Lin Li Department of Physics and Astronomy, Clemson University SC, United States, 29631 Email: lli5@g.clemson.edu Phone: 1-636-364-8518 Education and work experience: Research Associate
Lipid Regulated Intramolecular Conformational Dynamics of SNARE-Protein Ykt6
Supplementary Information for: Lipid Regulated Intramolecular Conformational Dynamics of SNARE-Protein Ykt6 Yawei Dai 1, 2, Markus Seeger 3, Jingwei Weng 4, Song Song 1, 2, Wenning Wang 4, Yan-Wen 1, 2,
Transport between cytosol and nucleus
of 60 3 Gated trans Lectures 9-15 MBLG 2071 The n GATED TRANSPORT transport between cytoplasm and nucleus (bidirectional) controlled by the nuclear pore complex active transport for macro molecules e.g.
Measurement of Dynamic trna Movement and Visualizing Ribosome Biogenesis Applications of Time resolved Electron Cryo microscopy
2011 TIGP CBMB Seminar Measurement of Dynamic trna Movement and Visualizing Ribosome Biogenesis Applications of Time resolved Electron Cryo microscopy Ribosome dynamics and trna movement by time resolved
GO ID GO term Number of members GO: translation 225 GO: nucleosome 50 GO: calcium ion binding 76 GO: structural
GO ID GO term Number of members GO:0006412 translation 225 GO:0000786 nucleosome 50 GO:0005509 calcium ion binding 76 GO:0003735 structural constituent of ribosome 170 GO:0019861 flagellum 23 GO:0005840
Tuesday 9/6/2018 Mike Mueckler
Tuesday 9/6/2018 Mike Mueckler mmueckler@wustl.edu Intracellular Targeting of Nascent Polypeptides Mitochondria are the Sites of Oxidative ATP Production Sugars Triglycerides Figure 14-10 Molecular Biology
Visualizing the transfer-messenger RNA as the ribosome resumes translation
Manuscript EMBO-2010-75355 Visualizing the transfer-messenger RNA as the ribosome resumes translation Jie Fu, Yaser Hashem, Iwona Wower, Jianlin Lei, Hstau Y. Liao, Christian Zwieb, Jacek Wower and Joachim
Machines with Moving Parts See How They Run.
NRAMM Workshop, CIMBio, La Jolla CA November 8-13, 2009 Machines with Moving Parts See How They Run. Alasdair C. Steven Laboratory of Structural Biology, NIAMS-NIH Sources of Variability in Electron Micrographs
Outline. Introduction, program and reference textbooks A few definition and facts Modeling of biological systems:
Introduction 1 Outline Introduction, program and reference textbooks A few definition and facts Modeling of biological systems: Models in space and time: from molecules to the cell Quantitative models
Copyright Mark Brandt, Ph.D A third method, cryogenic electron microscopy has seen increasing use over the past few years.
Structure Determination and Sequence Analysis The vast majority of the experimentally determined three-dimensional protein structures have been solved by one of two methods: X-ray diffraction and Nuclear
Supporting Online Material for
www.sciencemag.org/cgi/content/full/310/5753/1513/dc1 Supporting Online Material for Structural Roles for Human Translation Factor eif3 in Initiation of Protein Synthesis Bunpote Siridechadilok, Christopher
Dynamic modular architecture of protein-protein interaction networks beyond the dichotomy of date and party hubs
Dynamic modular architecture of protein-protein interaction networks beyond the dichotomy of date and party hubs Xiao Chang 1,#, Tao Xu 2,#, Yun Li 3, Kai Wang 1,4,5,* 1 Zilkha Neurogenetic Institute,
How Do We Get Oxygen From the Air Into Our Cells?
Name: Per: Date How Do We Get Oxygen From the Air Into Our Cells? Diffusion, Osmosis and Active Transport DAY 1 Read and follow the steps below to get started: 1. Go to the following website to learn more
Patrick: An Introduction to Medicinal Chemistry 5e Chapter 04
01) Which of the following statements is not true about receptors? a. Most receptors are proteins situated inside the cell. b. Receptors contain a hollow or cleft on their surface which is known as a binding
Chapter 1. DNA is made from the building blocks adenine, guanine, cytosine, and. Answer: d
Chapter 1 1. Matching Questions DNA is made from the building blocks adenine, guanine, cytosine, and. Answer: d 2. Matching Questions : Unbranched polymer that, when folded into its three-dimensional shape,
Chapter 4 A Tour of the Cell. The human body is made up of trillions of cells many of which are specialized - Muscle cells
Chapter 4 A Tour of the Cell State Standards Standard 1.c. Standard 1.e. Introduction to Cells Organisms are either - Single-celled, such as - Multicelled, such as The human body is made up of trillions
Turns sunlight, water & carbon dioxide (CO 2 ) into sugar & oxygen through photosynthesis
CELL PART/ ORGANELLE FUNCTION (what it does) PICTURE Plant, Animal, or Both Cell Membrane controls what goes in & out of the cell protects the cell Nucleus directs all the cell s activities contains cell
Introduction The gramicidin A (ga) channel forms by head-to-head association of two monomers at their amino termini, one from each bilayer leaflet. Th
Abstract When conductive, gramicidin monomers are linked by six hydrogen bonds. To understand the details of dissociation and how the channel transits from a state with 6H bonds to ones with 4H bonds or
Structure, Dynamics and Function of Membrane Proteins using 4D Electron Microscopy
Structure, Dynamics and Function of Membrane Proteins using 4D Electron Microscopy Anthony W. P. Fitzpatrick Department of Chemistry, Lensfield Road, Cambridge, CB2 1EW My research aims to understand the
122-Biology Guide-5thPass 12/06/14. Topic 1 An overview of the topic
Topic 1 http://bioichiban.blogspot.com Cellular Functions 1.1 The eukaryotic cell* An overview of the topic Key idea 1: Cell Organelles Key idea 2: Plasma Membrane Key idea 3: Transport Across Membrane
Biology. 7-2 Eukaryotic Cell Structure 10/29/2013. Eukaryotic Cell Structures
Biology Biology 1of 49 2of 49 Eukaryotic Cell Structures Eukaryotic Cell Structures Structures within a eukaryotic cell that perform important cellular functions are known as organelles. Cell biologists
Cellular Transport. 1. Transport to and across the membrane 1a. Transport of small molecules and ions 1b. Transport of proteins
Transport Processes Cellular Transport 1. Transport to and across the membrane 1a. Transport of small molecules and ions 1b. Transport of proteins 2. Vesicular transport 3. Transport through the nuclear
Slide 1. Slide 2. Slide 3. Chapter 4 A Tour of the Cell. State Standards. Introduction to Cells. Standard 1.c. Standard 1.e.
Slide 1 Chapter 4 A Tour of the Cell Slide 2 State Standards Standard 1.c. Standard 1.e. Slide 3 Introduction to Cells Organisms are either - Single-celled, such as - Multicelled, such as The human body
Concept 6.1 To study cells, biologists use microscopes and the tools of biochemistry
Name Period Chapter 6: A Tour of the Cell Concept 6.1 To study cells, biologists use microscopes and the tools of biochemistry 1. The study of cells has been limited by their small size, and so they were
Organisms: We will need to have some examples in mind for our spherical cows.
Lecture 4: Structure and Composition (Sept. 15) 4.1 Reading Assignment for Lectures 3-4: Phillips, Kondev, Theriot (PKT), Chapter 2 Problem Set 1 (due Sept. 24) now posted on the website. Cellular materials: