( BTH) Stud ies on Induction Resistance to P sedope ronospo ra cubensis by BTH in Cucum ber Seedlings

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1 2006, 33 (2) : Acta Horticulturae Sinica ( BTH) ( 1, ; 2, ) : 015 mmol/l BTH ( ) h (7 d) 2 4 d, BTH,, 48 h 7 d 21032, BTH, : ; ; BTH; ; ; : S : A : X (2006) Stud ies on Induction Resistance to P sedope ronospo ra cubensis by BTH in Cucum ber Seedlings L i Yuhong 1, Cheng Zhihui 13, Chen Peng 2, and Chen Xiaoguang 1 ( 1 College of Horticulture, N orthw est A & F U niversity, Yangling , China; versity, Yangling , China) 2 College of L ife Sciences, N orthw est A & F U ni2 Abstract: 015 mmol/l BTH was used in seedling with two true leaves 4, 8 and 12 h, or 1, 2, 4 and 7 days before or 4 and 2 days after inoculation with P1cubensis. Results obtained showed that BTH had not a curative effect but could induce local and system ic resistance in cucumber against the downy m ildew. Exp res2 sion of system ic resistance in leaves was detectable 48 h after treatm ent and continued for at least 7 days. Chitinase and 21032glucanase activities analysis showed that induced resistance in cucumber leaves was accompanied by a system ic increasing in both enzymes activities. Two enzym es were related to resistance to downy m ildew in cucumber. Key words: Cucumber downy m ildew; BTH; Induced resistance; Chitinase; 21032glucanase,,,,, 1 4 BTH ( ), 1 4, BTH, 1 111, 20 35, 2 : ; : : (2004BA516A09) ; ; (042M083) 3 Author for correspondence ( E2mail: chengzh@public1xa1sn1cn)

2 2 : (BTH) BTH BTH,, 2 d 2 4 d 015 mmol/l BTH ( ) 1, (,, ), 7 d 1 2 3, 30 5, BF CF BTH 1, BS CS BTH BTH, h, 015 mmol/l BTH 10 ml ( ), BTH, 20 3 h 2 ( 50 L),, , 3,, ( % ) 114 BTH 015 mmol/l BTH ( ) 1, h 14 ( ), 3, BTH 21032, 1, 015 mmol/l BTH ( ) 1 2, BTH ( ) 1 2 d 3, g, 5 ml (0105 mol/l, ph 510), (3 ) 6, 1 (U ) 1 mol N , 1 (U) 1 mol BTH 1, 2 d BTH 1 2, 2 d 4 d BTH, 1 2, 2 d BTH, 015 mmol/l BTH, 3 h 20% 2112%, 6 h 6017% 5419%, 9 h 8513% 9015% BTH 1 BTH 3 3 1% F ig. 1 Effect of BTH solution s trea tm en t before or after inocula tion on the downy m ildew d isea se index in the cucum ber CS: The second true leaves after treatment with water; 3 3 : Denotes at 1% level signification.

3 280 33, BTH, 212 BTH BTH, ( 2, 3) BTH h, 1 2 ; BTH 24 h, 1,, BTH 24 h BTH 48 h, 1 2 BTH 48 h 168 h (7 d), 1 2, BTH 168 h (7 d) 2 BTH 1 3 BTH 2 3 5% ; 3 3 1% 3 5% ; 3 3 1% Fig. 2 D isease index on the first leaves of cucumber seedlings after different tim e interva ls between BTH trea tm ent and inocula tion 3 : Denotes at 5% level signification; 3 3 : Denotes at 1% level signification. 213 BTH Fig. 3 D isease index on the second leaves of cucumber seedlings after differen t tim e interva ls between BTH treatm ent and inocula tion 3 : Denotes at 5% level signification; 3 3 : Denotes at 1% level signification., BTH 1 2 ( 4), BTH, 4 BTH F ig. 4 Activ ities of ch itina se in cucum ber leaves after BTH trea tm en t 5 F ig. 5 Activ ities of ch itina se in cucum ber leaves after inocula tion

4 2 : (BTH) 281, ( 5) BTH d, 7 d ; 2, 214 BTH 21032, BTH ( 6), 2, BTH 4 d 7 d BTH 21032, 21032, ( 7) BTH d, 7 d ; 2, 21032, BTH F ig. 6 Activ ities of 21032glucana se in the cucum ber leaves after BTH trea tm en t F ig. 7 Activ ities of 21032glucana se in the cucum ber leaves after inocula tion 3, BTH BTH 1 2, BTH BTH 7 d ( 2, 3) BTH 24 h,, 48 h BTH (48 h) (24 h), ( SAR) 2, , 21032, , 9,,, 8 BTH, BTH, ( 4,

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