: O 646 : A H 2 SO 4 20 ( SCE). ( PBS) : 0. 14mol NaCl, 3mmol KCl, mol Na 2 HPO 4, 7mmol KH 2 PO 4,

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1 ELECTROCHEM ISTRY Vol. 14 No. 1 Feb : (2008) Pt 1, 2, 2, 2, 2, 1 1, 23, (1.,, ; 2.,, ) : ( electrochem ical in2situ FTIR ) Pt. : Pt, ; 2 ; V ( vs. SCE) V ( vs. SCE),. : ; ; ; : O 646 : A.,.,,, [ 1 ].,. [ 228 ],,., [ 9 ]. Pt Pt ( = 5. 0 mm ),, m A l 2 O 3,,0. 1mol/L H 2 SO 4 20,, ( SCE). Pt 4 mg/ml 60 m in ( Sigma ),,. ( PBS) : 0. 14mol NaCl, 3mmol KCl, mol Na 2 HPO 4, 7mmol KH 2 PO 4, 1000 ml, NaOH ph = :PBS 4mg/ ml. 2 : g K 3 [ Fe (CN) 6 ], g K 4 [ Fe (CN ) 6 ] 3H 2 O 0. 5mol/L KCl250 ml 2mmol/L [ Fe (CN ) 6 ] 4 - / [ Fe (CN) 6 ] CH I2630 ( ),, Pt,, ( SCE). : , : , E2mail: superwgj@263. net; Namba_wan@ home. swjtu. edu. cn (NSFC: No ), (No ), 973 (No. 2005CB623904), (No ).

2 1 : Pt 57 MCT2B Gopher N ICO2 LET 730, CaF 2, ( ) [ 10 ] (MS2FTIR ), ( E r) R ( E r), ( E s) R ( E s). ( R /R ) : R /R = (R ( E s) - R ( E r) ) /R ( E r) (1), 1000, 8 cm - 1. E s, PtPBS 4 mg/ml PBS., PBS, 100 mv / s ( a), I E V ( vs. SCE), V. PBS ( b),, 500 mv / s( 1B ),,,., V ( vs. SCE) PBS, [ 5 ],. 1, Sawyer [ 9 ], V Pt ( ). (340Kda),,, [ 11 ]. 2 Pt 2., Pt V /( Epa = V, Epc = V ) ; Pt, / ( Epa = V, Epc = 2 2Pt ( a) Pt ( b) Fig. 2 Cyclic voltammogram s of the bare Pt electrode ( a) and the Pt modified by bovine fibrinogen electrode ( b ) in 2mmol/L ( K 3 [ Fe ( CN ) 6 ] + K 4 [ Fe (CN) 6 ]. 3H 2 O) mol/L KCl scan rate : 100mV / s 1Pt Fig. 1 Cyclic voltammogram s of the Pt electrode in PBS( a) and in PBS containing 4mg/mL bovine fibrinogen ( b) scan rate /mv s - 1 : A) 100; B) 500

3 V),,Ipa 50% ( A /cm ma /cm 2 ) Pt4 mg/ml, ( E r) V, E s V ( V ) 1. 1 V., b, c, da.,. 3b V ( vs. SCE), 990 cm - 1,C S S O S S,, 29 3 (A B C ) [ 8 ] ; cm V ( vs. SCE), C O C N, C N [ 12 ] ; 0. 7V 1. 1V ( vs. SCE), 1161 cm - 1,C O, C O,3c, cm - 1 ( 1573 cm cm - 1 ), [ 3, 8 ], C H (1550 cm - 1 ) 2sheet ( cm - 1 ). C H, 5 (ATR2FTIR),., 2337 cm cm - 1 ( 3d), N H +, 3 Pt4mg/mL PBS Fig. 3 In2situ FTIR spectra recorded on the Pt electrode in PBS containing 4mg/mL bovine fibrinogen E r = V, potential scan range E s/v: a) ; b) ; c, d) , wave number/ cm - 1, a) ; b) ; c) ; d)

4 1 : Pt 59, [ 13 ] cm - 1 C H N H [ 14, 8 ], 5, V, [ 1 ]. 3 1) Pt, ; 2. 2) V ( vs. SCE),,,.,. ( References) : [ 1 ] Cheng Q ing ( ), W ang Guo2bin (). B lood material interaction [ J ]. neering, 2001, 2 (3) : 33. ShangHai B iomedical Engi2 [ 2 ] Peiqing, Ana S V iana, Luisa M Abrantes, et al. Ado2 sorpotion of human serum album in onto gold: a com2 bined electrochem ical and ellip sometric study[ J ]. Jour2 nal of Colloid and Interfaces Science, 2004, 279: 95. [ 3 ] Sibel Tunc,Manfred F Matiz, Gerald Steiner, et al. In situ conformational analysis of fibrinogen adsorbed on Si surfaces[ J ]. Colloids and Surfaces B, 2005, 42: 219. [ 4 ] Robert T TGettens, Jeremy L Gilbert. Quantification of fibrinogen adsorp tion onto 316L stainless steel [ J ]. Journal of B iomedicalmaterials, 2006, 11: 465. [ 5 ] Ramasamyl N, Ranganathan M, Duic L, et al. Electro2 chem ical behavior of blood coagulation factors [ J ]. J Electrochem Soc, 1973, 120: 354. [ 6 ] Douglas R Jackson, Sasha Omanovic, Sharon G Ros2 coe. Electrochem ical studies of the assorp tion behavior of serum p roteins on titanium [ J ]. Langm iur, 2000, 16: [ 7 ] W ang J ie, Chen Xiao - yun, Mattew L Clarke, et al. V ibrational spectroscop ic studies on fibrinogen adsorp2 tion at polystyrene /p rotein solution interfaces: Hydro2 phobic side chain and secondary structure changes[ J ]. J Phys Chem B, 2006, 110: [ 8 ] Matthew L Clarke, W ang J ie, Chen Zhan. Conformation2 al changes of fibrinogen after adsorp tion [ J ]. J Phys Chem B, 2005, 109 ( 46) : [ 9 ] Sawyer P N. Significance of electrochem ical phenomena in intravascular thrombosis [ J ]. Nature, 1965, 206 (2) : [ 10 ] Sun Shi2gang, Gong Hui. Research methods of solid catalyst[ J ]. Petrochem ical Industry, 2001, 30 ( 10) : 806. [ 11 ] Luo Guo2an (), W ang Zong2hua (), W ang Yi2m ing(). App lications and fabrication of the bio2compatible electrode [M ]. Beijing: Science Publishing House, [ 12 ] Patrick Vermette, V irginie Gauvreau, M ichel Pezolet, et al. A lbum in and fibrinogen adsorp tion onto phos2 phatidylcholine monolayers investigated by fourier transform infrared[ J ]. Colloids and Surfaces B, 2003, 29: 285. [ 13 ] Xing Xu2ying (), Chen Shi2di (), Shen Si2yun (). The use guide of infrared spectra [M ]. Beijing: Science Publishing House, [ 14 ] Stamatin L, Cristescu R, Socol G, et al. Laser deposi2 ton of fibrinogen blood p roteins thin film s by matrix as2 sisted pulsed laser evaporation [ J ]. App lied Surface Science, 2005, 248: 422.

5 Interfacial Electrochem ical Behavior of Fibrinogen on Pt Electrode CHEN Song2mei 1, 2, ZHOU J ian2zhang 2, L IN Zhong2hua 2, ZENG Dong2mei 2, HUANG Nan 1 1, 23, WAN Guo2jiang (1. Key L ab. of advanced Technology of M aterials of Education M inistry of China, Collge of M a terials Science and Engineering, Southw est J iaotong U niversity, Chengdu , China; 2. S tate Key L ab. of Physical Chem istry of the Solid Surface, D ept. of Chem istry, X iam en U niversity, X iam en , Fujian, China) Abstract: The interfacial electrochem ical behaviors of fibrinogen on Pt electrode in phosphate buffered solution ( PB S) was investigated by using cyclic voltamm etry and electrochem ical in2situ FTIR spectroscopy. The cyclic voltamm etry results demonstrated that the fibrinogen adsorb on Pt electrode and impede the interfacial charge transfer of hydrogen ion reaction p rocess as well as of oxygen reduction p rocess, which is increased w ith higher scan rate. The cyclic voltammetry spectra of p latinum absorbed with fibrinogen in K 3 [ Fe (CN ) 6 ] and K 4 [ Fe (CN) 6 ] 3H 2 O solutions shows that the fibrinogen can partly decrease the oxidation and reduction current peaks. There is no specific anodic and cathodic current peaks found to attributed from fibrinogen w ithin potential scanned rang of V ( vs. SCE). Nevertheless, electrochem ical in2situ FTIR spectra indicate the fibrin2 ogen undergo chem ical reaction when which anodic potential higher than V ( vs. SCE). Key words: fibrinogen; electrochem ical in2situ FTIRS; interfacial electrochem ical behavior; coagulation