NO - : S511 : A : (2005) Compa rison of nitra te utiliza tion by four diffe rent rice (O ryza sa tiva L.

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1 2005, 28 (1) : Journal of N anjing A gricultural U niversity NO -,,,, 3 (, ) : 4 ( ) (NRA) ( GSA), 1 mmol L - 1 NH + 4,, 1 mmol L - 1 NO d,,, 6, 57 ; NRA GSA, NRA 5817%, GSA 3416%, GSA 312, NO - : ; (NRA) ; ( GSA) ; NO - 3 ; : S511 : A : (2005) Compa rison of nitra te utiliza tion by four diffe rent rice (O ryza sa tiva L. ) cultiva rs CAO Yun, FAN Xiao2rong, J IA L i2jun, YIN Xiao2m ing, SHEN Q i2rong 3 (College of Resourses and Environmental Sciences, Nanjing Agricultural University, Nanjing , China) Ab stra c t: Four rice cultivars were grown in solution culture and their growth and utilization of nitrate (NO - 3 (NH + 4 ) and ammonium ) was compared. The results obtained were as follows. A ll the four rice cultivars, Shanyou 63, Yangdao 6, Siyou 917 and Nongken 57 grew well and p roduced sim ilar amounts of biomass in 1 mmol L - 1 ammonium as the only N source. In contrast, they behaved differently when supp lied with 1 mmol L - 1 NO - 3. Yangdao 6 p roduced the greatest biomass while Nongken 57 the lowest growth among the four cultivars in 1 mmol L - 1 NO - 3. Indica rice had higher nitrate reductase activity (NRA) and glutam ine syn2 thetase activity ( GSA) than Japonica rice in 1 mmol L - 1 NO - 3. The leaf NRA and GSA of Indica rice was 5817% and 3416% higher than that of Japonica rice, respectively. The root GSA of Indica rice was 312 times higher than Japonica rice. A ll the results suggested that Indica rice had a greater ability to utilize nitrate than Japonica, which is very useful for the further study of differ2 ences in NO - 3 utilization from molecular app roach. Key wo rd s: O ryza sativa L. ; nitrate reductase activity (NRA) ; glutam ine synthetase activity ( GSA) ; NO - 3 ; utilization,, NO - 3 2N [ 1 3 ], NO - 3 2N NO - 3, [ 2, 4 ], NO - 3, NO - 3 NO - [ 3 ], NO -, NH + 4, NO -,, (O ryza sativa rl. ) : 63 ( H2I) 6 ( I) : : ( ) : (1981 E2mail: ), 3 Corresponding author: (1957 ),,, qrshen@ njau1edu1cn ' China Academic Journal Electronic Publishing House. All rights reserved.

2 1 : NO ( H2J) 57 ( J) % ( ) H 2 O 2 30 m in, h, 5 L, 12, 3 15 h, 29, 34, 65% 5% Ca (NO 3 ) 2 NH 4 Cl 1 mmol L - 1 NO mmol L - 1 NH + 4 : 2 mmol L - 1 K 2 SO 4, 2 mmol L - 1 MgSO 4, 1 mmol L - 1 CaCl 2, 013 mmol L - 1 NaH 2 PO 4, 40 mol L - 1 Fe2EDTA, 9 mol L - 1 MnCl 2, 25 mol L - 1 (NH 4 ) 6 Mo 7 O 24, 20 mol L - 1 H 3 BO 3, 115 mol L - 1 ZnSO 4, 115 mol L - 1 CuSO 4 1 mmol L - 1 Na 2 SiO 7 mol L - 1 (DCD), 1 mol L - 1 HCl 1 mol L - 1 NaOH ph 515, 2 d 4, NO - 3 (NRA) ( GSA) NO - 3 [ 5 ] 1 g,, 25 ml,, 30 m in; 100 ml, 1 g, ; NO NRA 28 d, [ 5, 6 ] NRA 10: 00 20: cm 1 g,, 10 m in - 20 : 5 mmol L - 1 EDTA 5 mmol L mol L - 1 (ph 817) 2 mg ml - 1 NADH : 5 ml,, 10 ml, r m in m in, 1 ml KNO ml NADH 014 ml, m in, 015 ml NADH, 015 ml 2, r m in m in, 540 nm 011 mol L - 1 (ph 714) NADH NO - 3 2N GSA 28 d, [ 6 ] GSA, g, ( ph 712, 015 mmol L - 1 EDTA, 50 mmol L - 1 K 2 SO 4 ),, r m in m in, 016 ml HCl 014 ml 014 ml ATP2Na 012 ml MgSO ml, 25 5 m in, 012 ml, m in 018 ml r m in m in 540 nm 1 mmol L (U ) NO - 1, 1 mmol L - 1 NO - 6 ( ), 57 ( ),,, , 6 ( ) 3, ; ( 6 63) 1 mmol L - 1 NH + 4, ( ) 212 NO - NO - 1 mmol L - 1 NO - 3, NO mol g - 1, NO mol g - 1 ( 1 A ) 6 NO - 3 ( 25 mol g - 1 ) 3 63 (

3 54 28 Cultivars 63 (H2I) Shanyou 63 6 ( I) Yangdao (H2J) Siyou (J) Nongken mm o l L - 1 NO - ( n = 4 ) Tab le 1 G row th o f fo u r rice cu ltiva rs in hyd ropo n ic cu ltu re w ith 1 mm o l L - 1 NO - 3 a s the N so u rce Fresh weight b a bc c D ry weight a a a a Root / cm Maximum length / % W ater content bcBC abAB aA bcC cC aA cC cC Fresh weight bB aA bBC bC D ry weight a a a a Shoot : Duncan s Note: A ll ANOVA was carried by SPSS, the 0105 and 0101 probability level, using Duncan s mutiple range test. respectively. /cm Plant height bB aA bB bB / % W ater content bB aA bB cC Data with symbols abc and ABC rep resent significant difference at ) NO -, NO - 3 NO %, 3 NO - 3, 4019%, 57 ( ) ; 4 ( 1 B) NO - 3, 1 1 mm o l L - 1 NO - NO - 3 (A) (B ) ( ) F ig11 N itra te co n ten t (A) a nd to ta l n itro ge n (B ) o f d iffe re n t rice cu ltiva rs g row n in 1 mm o l L - 1 NO - 3 cu ltra l system ( ca lcu la te d by fre sh w e igh t) ( : H2I: 63 Shanyou 63; I: 6 Yangdao 6; H2J: 917 Siyou 917; J: 57 Nongken 57) 213 NO - NRA GSA, (NRA) mol g - 1 h - 1 ( 2 A), 2 1 mm o l L - 1 NO - NRA (A) GSA (B ) F ig12 N itra te reduc ta se a c tivity in le ave s (A) a nd g lu tam ine syn the ta se ac tivity in lea ve s and roo ts (B ) am o ng d iffe ren t rice cu ltiva rs in 1 mm o l L - 1 NO - 3 cu ltra l system

4 1 : NO NRA, 5817%, 63 NRA 8619%, 6 ; NRA 2414% NRA,, 63 6 NRA mol g - 1 h - 1, ( ) 2 B GSA GSA U, 917 GSA, 6 GSA NRA, GSA, 3416% GSA, GSA 312, 57, 3 1 mmol L - 1 NO -, 4 1 mmol L - 1 NH + 4,, [ 7 9 ], NO - 3 2N, NO - 3 2N, ( 6 63) ( ), [ 2, 4 ] [ 10 ],,,,, ( ) [ 11 ], NO - 3 ( 1) NO - 3, 63, NO -, NO - 3,, NO - [ 12 ], NO -, N iedziela [ 13 ] NO - 3, NO -, 4 NO - NRA ( 1, 2) [ 14 ], [ 15 ] NRA, NRA [ 11 ], NRA [ 9 ], NRA, NO - 3 2N ( GS), GS NH 3 ATP, GS2GOGAT [ 16 ] Lam [ 17 ], NO - 3 2N GS mrna NO - 3, GS NO - GS NO -,, NH + 4 [ 18 ] GSA NRA ( 2) 917 GSA 3, 57, 1 mmol L - 1 NO - ( 1), : [ 1 ],,. [ J ]., 2004, 41 (5) : [ 2 ],. NH + 4 NO - 3 [ J ]., 1991, 22 (5) : [ 3 ],,,. NO - NH + 4 [ J ]., 2003, 36 ( 3) : [ 4 ] B riones A M, Okabe S, Umem iya Y, et al. Ammonia2oxidizing bacteria on root biofilm s and their possible contribution to N use efficiency of different rice cultivars [ J ]. Plant and Soil, 2003, 250: [ 5 ]. [M ]. :,

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