The 4-Source Ion Trap

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1 Introduction ur research program is devoted to the study of gaseous macro-ions applied within the context of mass spectrometry to challenging analysis prolems. ur work involves instrument development, ion chemistry studies, and applications to analytical prolems. This poster summarizes our recent activities in these areas.

2 Instrumentation Finnigan (ITMS TM ) dual source ion trap mass spectrometer Finnigan (ITMS TM ) 4-source ion trap mass spectrometer Modified Q STAR quadrupole time-of-flight mass spectrometer Two modified Q TRAP linear ion trap mass spectrometers Multi-Source linear ion trap Cold-ion spectroscopy tandem mass spectrometer

3 The 4-Source Ion Trap Glow discharge source Turning quad -ESI source ESI / Glow Discharge Source Ion trap Turo pump 400 L/s Transfer optics (tue lens / linear quad) Turo pump 400 L/s ESI source

4 Modified Dueling Source 3D Ion Trap Finnigan Electronics Frequency Synthesizer TTL1 ut RF Power Supply V -V ASGDI Source DC Pulse Generator Amplifier Custom Switch Box ESI Source

5 Multi-Source Linear Ion Trap 500 mtorr Glow Discharge 1 mtorr Turning Quad CEM Quadrupole Ion Trap Electrostatic Lenses ESI Tip

6 Cold-Ion Spectroscopy Tandem Mass Spectrometer Joint project with the Laoratory of Timothy Zwier Detector 4K Cold Trap Laser Pulse Source B (Glow Discharge) Source A (ESI)

7 Modified Q Star Quadrupole Time-of-Flight Leak-in Setup LIT ion/ion Reactor TF Analyzer rifice Curtain Plate Plate

8 Modified QTRAP Linear Ion Trap 2 CAD Gas Aux AC Reagent inlet Analyte inlet RF Skimmer IQ1 ST IQ2 IQ3 rifice Q0 Q1 Q2 Q3 Exit lens Deflector Reagent inlet Detector Pulsed Triple Ionization Source

9 Modified MALDI-QTRAP4000 Linear Ion Trap Laser Beam

10 Custom Components High power rf supplies Ale to drive LITs, QIT, and mass resolving Quadrupoles Low power rf supplies Durale tue-ased design for ion optical devices Scanale transistor supplies for simple mass analysis Ion funnel Small, simple design Significant signal gains on homeuilt interface Roust interface

11 Chemistry Unimolecular Dissociation Reactions Peptides Proteins ucleic Acids Carohydrates Ion/Ion Reactions Proton Transfer (PT) Electron Transfer (ET) Specific Covalent Chemistry Ion/Molecule Reaction Treatment of Electrosprayed Droplets

12 Collision-Induced Dissociation (CID) a 1 c 1 a 2 c R 1 R 2 R 3 H 2 CH C H CH C H CH CH Reagent anion e- - x 2 Excitation increases ion displacement from the trap center, wherey ions undergo increases in kinetic energy. Collisions with the ath gas convert kinetic energy to internal energy (slow heating). Electron Transfer Dissociation (ETD) a 1 1 y 2 c 1 z 2 a 2 x 1 y 1 R 1 R 2 R 3 H 2 CH C H CH C H CH CH 2 c 2 z 1 x 2 y 2 z 2 x 1 y 1 z 1 Less sequence dependence than CID (wider sequence coverage) Does not cleave laile post translational modifications

13 Electron Transfer Reaction etween Azoenzene and Disulfide-linked Peptide [M3H] 3 Relative Aundance, % [A] [A-2H] [BH] [B] Pep V: (q)a G C K(q)-Me S S (q )T F T S C-Me A: A-Chain B: B-Chain Ac 1 Bc 1 Az 1 Bc 2 [B-S-H] Bc 3 Bc [A-SH 2 ] [AS] [BSH] H 3 C H 3 C ABz 2 (CH 2 ) 3 H _ CH 3

14 DC CID: Fragmentation vs Time

15 DC CID: A Broadand Application Top: DC CID of romocriptine. 40 V DDC, 7.5 ms, low mass cutoff = 117. Middle: DC CID of YGGFL V DDC, 10 ms, low mass cutoff = 51 Th. Bottom: DC CID of uiquitin V DDC, 10 ms, low mass cutoff = 234 Th.

16 Relative Aundance Relative Aundance Ion/Ion Reactions of Transition Metal Complex Cations with Multiply Charged ligodeoxynucleotide Anions 100 a) (A 6 3H) 3 [Cu II (phen) 2 ] 2 (A 6 3H) 3 * (A 6 3H) 2 a) (A 6 3H) 3 [Cu II (phen) 3 ] 2 # [Cu II (A 6 3H)] 100 ~ * (A 6 3H) 3 (x 9.1) (A 6 3H) 2 w 1 (A 6 2 3H) 2 [Cu II (A 6 3H)] ) (A 6 3H) 3 [Co II (phen) 2 ] (A 6 3H) 2 [Co II (A 3H)] ) (A 6 3H) 3 [Co II (phen) 3 ] 2 [Co II (A 6 3H)]

17 Intenisty (Ar. Units) Intensity (Ar. Units) ESI of a DA Mixture, pd(a)40-60 Proton Transfer Charge Reduction of Multiply- Charged pd(a)40-60 Anions pda 41 pda 42 pda 43 pda 44 pda 45 pda 46 pda 47 pda 48 pda 49 pda 50 pda 51 pda 52 pda 53 pda 54 pda 55 pda 56 pda 57 pda 58 pda 59 pda 60 pda 40

18 Aundance Ion Parking [M15H] [M19H] 19 [M11H] 11 [M17H] [M21H] [M12H] 12 [M10H] 10 [M14H] a) ) c) Mass Spectra of Porcine Elastase Acquired in (a) Pre Ion/Ion, () Post Ion/Ion and (c) Ion Parking Modes

19 Charge Inversion Process n- H H H H H H H H H H H H H Polyamidoamine (PAMAM) Dendrimer Anion m - H - H 2 H 2 H 2 H 2 H 2 H 2 Peptide Ion (Bradykinin) H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 - H - H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 1,4-Diaminoutane (DAB) Dendrimer Cation H 2 H 2 H 2 H 2 H 2 H 2 H 2 2 2H

20 Aundance (Ar. Units) Sequential Information via Covalent Modification made in the Gas Phase 9e6 a) D R V Y I H P F [M -H 2 ] - 6e6 CID of modified angiotensin with 4-formyl-enzene disulfonic acid [M ] - 3e6 0 [FBDSA-H] y 7 1.6e8 8e7 ) CID of unmodified angiotensin y 7 [M-H]

21 Relative Aundance (Ar. Unit) Inter-Molecular Cross-Linking in the Gas-Phase 6.2x E6 3.1x E6 (a) Ion Trap CID of [YGGFLKBS 3-2a-(sulfo-HS)KKKKKKKKKK2H] 2 [M1BS 3-2a-(sulfo-HS)M22H] 2 -(sulfo-hs) -H 2 9 * 2 * denotes KKKKKKKKKK fragment ions cross-linked to YGGFLK 0 1.4x E6 7.0x E () 7 * 2 y 7 * 2 8 * 2 y 8 * 2 1 * y 1 * 9 * 2 y 9 * 2 -H 2 [M1M2BS 3-2a-2(sulfo-HS)2H] 2 x2.5 2 * y 2 * 9 3 * y 3 * MS 3 of [YGGFLKBS 3-2a- 2(sulfo-HS)KKKKKKKKKK2H] 2 4 * y 4 * 5 * y 5 * 6 * y 6 *

22 Aundance (Ar. Units) Charge Inversion/ETD of a Phosphopeptide Charge Inversion - - H -1 Phosphopeptide Anion H 2 H 2 H 2 H H 2 H 2 2 H H 2 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H H 2 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 H 2 7 H 2 7 DAB Dendrimer G4 Cation ETD 2H 2 2 Phosphopeptide Cation -1 Azoenzene Anion -

23 Intensity (Ar. Unit) Acid Vapor Introduction for Removal of Metal Counter- Ions of Various DA 12mers 4a 5a 3a 2a [M3H] 3 a 6a 7a nesi o Leak 8a 9a 10a [M-4H] 4- a K a -nesi o Leak Ka 3a (a) (d) [M3H] 3 a HCl Leak [M-4H] 4- Acetic Acid Leak () 2a 3a (e) a K (c) a 2a [M3H] 3 3a 4a 5a 6a TFA Leak (f) [M-4H] 4- Formic Acid Leak

24 Intensity (Ar. Unit) 6-5 Protein Folding Base Leak-in Acid Leak-in 9-7 Cytochrome c 100% H 2 16 Acid Leak-in Base Leak-in -10 Protein Unfolding

25 Applications Protein Mixture Analysis Dataase-Assisted Protein Identification Post-Translational Modification (PTM) Analysis

26 Aundance Top-Down Protein Identification Aundance Protein ESI Ionization & accumulation Precursor ion purification and concentration via ion parking Ion/Ion chemistry Dissociation Charge state reduction Ion/Ion chemistry Protein Dataase search Protein Identification

27 Relative Informing Power of ESI Based Top-down Approaches ESI High resolution 1 Post ion/ion 1 1

28 Aundance (Ar. Units) Modified DA Sequencing H A Me P SH C ITCID Ps 2 -Me RA 6-mer Hz_180mV c 3 2- Me P H C Me P SH G Me P H A P Me200 SH [M-AH] G 50 H Me 0 y 1 - # 1 a 1 y1 x - c w - 1 c a 2 -B y 3 2 c 3 2 x 3 2- # # d 5 3- x 5 3- [M-AH] 3- # # z 2 y 4 2- c 2 - z 4 2- c 4 2- x2 - a 5- -B 5 3- ITCID Ps 2 -Me RA 6-mer Hz_250mV y 4 2 y 2 c 2 w 2 [M-AH] 2- - y # y 5 2 x 5 2 [M-GH] 2 [M-CH] 2 x 3 - # - internal fragment y 3 x 3

29 Aundance Post Ion-Ion Reaction MS/MS Spectra of the [M 9H] 9 Ion of α-synuclein y y 3 y y / y 7 - y 9 y y y / y 16 y 14 y 20 y 15 y y y [M2H] 2 46, 46 * 42, 42 * y y 49, y 49 * 2 species / 2 60 *- 62 */ 55, 119 y * / 64 * 63 y / 65 * / y 64,y 66, y 68,y 68 * y / y / 66 * / 73 *, y 70, y 74, y 74 * / y 69, *- 83 */ y 79,y , 87 */y / 104 * 100 y y / * 92 y y 94 y / 115 *, 116 *, 118 */ y y / / 105 * 109 *, 110 *, 112 * */ 107 / y106 - y 108, y * y / 119 * y / 121 *, 122 *,/ 125 *- 127 */ y y 120 / y y / 131 */y / 134 *- 137 * [MH] y

30 Macro-Ionophiles From -terminus to C-terminus: Scott A. McLuckey Ken Chanthamontri Corrine DeMuth Christine Fisher Yang Gao Josh Gilert Kerry Hassell Ryan Hilger Anastasia Kharlamova Carl Luongo Marija Mentinova William McGee Boone Prentice James Redwine Jessica Espy John Stutzman Ian We CH H 3