What is Tandem Mass Spectrometry? (MS/MS)
|
|
|
- Anissa Brown
- 6 years ago
- Views:
Transcription
1 What is Tandem Mass Spectrometry? (MS/MS) Activate MS1 MS2 Gas Surface Photons Electrons
2
3 Major difference between MS and MS/MS: two analysis steps Ionization Analysis MS: Collide with target to produce fragments MS/MS: Ionization Selection Activation Analysis
4 Tandem Mass Spectrometry Widely Used Tool Many Instrument Types Many Applications
5 Why/when is MS/MS used? Why hook two mass analyzers together?
6 MS/MS is chemically selective -provides structural information on individual components of mixtures
7 Newborn Screening by MS/MS Currently, Neo Gen Screening monitors more than 60 chemical and chemical relationships in a single test. Tandem Mass Spectrometry measures these indicators or markers in a typical 2- minute run.
8 Proteomics Enzyme HPLC MS Proteins Peptide m/z Protein 1 Protein 2 SIYDGK FWSEFR TLLHPYK Peptide Sequence MS/MS
9 MS/MS often produces different spectra for isomers isomer distinction CH 3 CH 2 OH +.. CH 2 CH 2 OH 2 + Wysocki, Kenttamaa JACS 1990, 112,
10 isomer distinction not! EIMS
11 isomer distinction MS/MS
12 MS/MS or MS n provides fragmentation of cold ions -ions that do not gain enough energy to fragment in the ionization process
13 Why MS/MS? mixture analysis, select each ion isomer distinction cold ionization methods provide limited energy to ion, no fragments
14 Reasons to understand MS/MS No ideal MS/MS exists Knowledge of differences and reasons for differences allows for Choice of proper instrument Proper interpretation of results Knowledge of limitations can lead to improved instruments or new approaches to solve problems
15 How is MS/MS accomplished? MS1 Activate MS2 Gas Surface Photons (UV or IR) Electrons
16 The activation step in MS/MS is actually two steps: Activation Unimolecular dissociation
17 Simulation of Two-Step Process Hase Lab, Texas Tech
18 Two-step Process: activation + unimolecular dissociation Internal energy distribution Molecule: Different reaction pathways Energy dependent rate constants: k(e)=ν ([E-E 0 ]/E) s-1 (overly simplified) Instrument Configuration: Time for reaction
19
20 How is MS/MS accomplished? Activate MS1 MS2 Tandem-in-space (distinct analyzers) Tandem-in-time (trapping instrument)
21
22 Tandem in Space Triple quadrupole Collisions with gas
23 Tandem in Space Triple quadrupole Collisions with gas
24 Tandem in Space Orbitrap (Q/collision cell/orbitrap) Collide with gas Mass select Analyze m/z Ionize
25 Quadrupole ion trap Tandem in time
26 Tandem in Time MS/MS T1 T2 T3 Collisions with gas msec dissociation
27 MSn
28 Current MS/MS instruments Tandem in Time (Trapping) QIT, LIT Tandem in Space QqQ QqLIT QqTOF TOF TOF Trap-Orbi, Q-Orbi, Q-ICR
29 Activation Methods Post Source Decay (PSD) - MALDI instruments Collision Induced Dissociation (CID) low energy (ev) high energy (kev) single collision multiple collisions Sustained Off-Resonance Irradiation (SORI) Resonance Excitation (RE) Infrared Multiphoton Dissociation (IRMPD) Electron Capture Dissociation (ECD) Electron Transfer Dissociation (ETD) Surface-Induced Dissociation (SID) Blackbody Infrared Radiative Dissociation (BIRD) 29
30 Electron Capture Dissociation [M + nh] n+ + e - [[ M + nh] (n-1)+ ]* fragments 30
31 or or z ion c ion Cooper H J, Håkansson H, Marshall A G, Mass Spectrom. Rev., 2005, 24,
32 Comparison of ECD and SORI-CAD in FTICR c and z ion series c 3 + c 2 + c 4 + c 5 + c 6 + c 8 + c 9 + c 10 + no loss of water, phosphate groups or phosphoric acid y 2 + y 4 + b 7 + b 9 + b 10 + Stensballe, et al. Rapid Commun. Mass Spectrom., 2000, 14,
33 Cleave Peptide Bonds without Cleaving Sugar 33 Cooper H J, Håkansson H, Marshall A G, Mass Spectrom. Rev., 2005, 24,
34 ECD for Peptide Bonds, IRMPD for Sugar ECD IRMPD Cooper H J, Håkansson H, Marshall A G, Mass Spectrom. Rev., 2005, 24,
35 Why are there so many MS/MS instrument types? Many ionization sources Many mass analyzers Sort by mass/charge with different resolution, different mass accuracy, different time frames for dissociation Many applications
36 Figures taken from several web sites and publications including: as noted on figures plus (not active) (not active) Edmond de Hoffmann JMS, 1996, 31, Tandem Mass Spectrometry: A Primer
MASS ANALYSER. Mass analysers - separate the ions according to their mass-to-charge ratio. sample. Vacuum pumps
ION ANALYZERS MASS ANALYSER sample Vacuum pumps Mass analysers - separate the ions according to their mass-to-charge ratio MASS ANALYSER Separate the ions according to their mass-to-charge ratio in space
Mass Spectrometry. Hyphenated Techniques GC-MS LC-MS and MS-MS
Mass Spectrometry Hyphenated Techniques GC-MS LC-MS and MS-MS Reasons for Using Chromatography with MS Mixture analysis by MS alone is difficult Fragmentation from ionization (EI or CI) Fragments from
for the Novice Mass Spectrometry (^>, John Greaves and John Roboz yc**' CRC Press J Taylor & Francis Group Boca Raton London New York
Mass Spectrometry for the Novice John Greaves and John Roboz (^>, yc**' CRC Press J Taylor & Francis Group Boca Raton London New York CRC Press is an imprint of the Taylor & Francis Croup, an informa business
Ion Activation in MS/MS. Ion Activation in MS/MS
Ion Activation in MS/MS Briefly introduce different activation methods Provide framework for understanding differences Árpád Somogyi OSU CCIC MSP Summer Workshop July 10, 2018 1 Ion Activation in MS/MS
Week 5: Fourier Tranform-based Mass Analyzers: FT-ICR and Orbitrap
Week 5: Fourier Tranform-based Mass Analyzers: FT-ICR and Orbitrap 1 Last Time Mass Analyzers; CAD and TOF mass analyzers: 2 Fourier Transforms A transform is when you change your analytical space without
Interazioni di ioni con elettroni (ECD, ETD) e fotoni (Ion spectroscopy) Gianluca Giorgi. via Aldo Moro Siena
Interazioni di ioni con elettroni (ECD, ETD) e fotoni (Ion spectroscopy) Gianluca Giorgi Università degli Studi di Siena Dipartimento di Biotecnologie, Chimica e Farmacia via Aldo Moro 53100 Siena e-mail:
MS/MS .LQGVRI0606([SHULPHQWV
0DVV6SHFWURPHWHUV Tandem Mass Spectrometry (MS/MS) :KDWLV0606" Mass spectrometers are commonly combined with separation devices such as gas chromatographs (GC) and liquid chromatographs (LC). The GC or
Types of Analyzers: Quadrupole: mass filter -part1
16 Types of Analyzers: Sector or double focusing: magnetic and electric Time-of-flight (TOF) Quadrupole (mass filter) Linear ion trap Quadrupole Ion Trap (3D trap) FTICR fourier transform ion cyclotron
Atomic masses. Atomic masses of elements. Atomic masses of isotopes. Nominal and exact atomic masses. Example: CO, N 2 ja C 2 H 4
High-Resolution Mass spectrometry (HR-MS, HRAM-MS) (FT mass spectrometry) MS that enables identifying elemental compositions (empirical formulas) from accurate m/z data 9.05.2017 1 Atomic masses (atomic
Fundamentals of Mass Spectrometry. Fundamentals of Mass Spectrometry. Learning Objective. Proteomics
Mass spectrometry (MS) is the technique for protein identification and analysis by production of charged molecular species in vacuum, and their separation by magnetic and electric fields based on mass
Overview of NETCHEM MSc & PhD courses: mass spectrometry in EFSC
AQualEER Chemistry faculty (Belgrade, Serbia, December 2017) Overview of NETCHEM MSc & PhD courses: mass spectrometry in EFSC Emeritus Prof. Dr Jean-Claude Tabet 1,2,3 1 Institut Parisien de Chimie Moléculaire
TANDEM MASS SPECTROSCOPY
TANDEM MASS SPECTROSCOPY 1 MASS SPECTROMETER TYPES OF MASS SPECTROMETER PRINCIPLE TANDEM MASS SPECTROMETER INSTRUMENTATION QUADRAPOLE MASS ANALYZER TRIPLE QUADRAPOLE MASS ANALYZER TIME OF FLIGHT MASS ANALYSER
LECTURE-11. Hybrid MS Configurations HANDOUT. As discussed in our previous lecture, mass spectrometry is by far the most versatile
LECTURE-11 Hybrid MS Configurations HANDOUT PREAMBLE As discussed in our previous lecture, mass spectrometry is by far the most versatile technique used in proteomics. We had also discussed some of the
(Refer Slide Time 00:09) (Refer Slide Time 00:13)
(Refer Slide Time 00:09) Mass Spectrometry Based Proteomics Professor Sanjeeva Srivastava Department of Biosciences and Bioengineering Indian Institute of Technology, Bombay Mod 02 Lecture Number 09 (Refer
Mass Spectrometry and Proteomics - Lecture 2 - Matthias Trost Newcastle University
Mass Spectrometry and Proteomics - Lecture 2 - Matthias Trost Newcastle University matthias.trost@ncl.ac.uk Previously: Resolution and other basics MALDI Electrospray 40 Lecture 2 Mass analysers Detectors
Qualitative Proteomics (how to obtain high-confidence high-throughput protein identification!)
Qualitative Proteomics (how to obtain high-confidence high-throughput protein identification!) James A. Mobley, Ph.D. Director of Research in Urology Associate Director of Mass Spectrometry (contact: mobleyja@uab.edu)
Introduction to the Q Trap LC/MS/MS System
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +1.847.913.0777 for Refurbished & Certified Lab Equipment ABI Q Trap LC/MS/MS Introduction to the Q Trap LC/MS/MS System The Q Trap
Chapter 2 Fundamentals of Ion Chemistry
Chapter 2 Fundamentals of Ion Chemistry Toshihiro Fujii 2.1 Termolecular Association Reactions 2.1.1 Association Reaction Mechanism It was experimentally found that the overall process of ion molecule
Identification of Human Hemoglobin Protein Variants Using Electrospray Ionization-Electron Transfer Dissociation Mass Spectrometry
Identification of Human Hemoglobin Protein Variants Using Electrospray Ionization-Electron Transfer Dissociation Mass Spectrometry Jonathan Williams Waters Corporation, Milford, MA, USA A P P L I C AT
SHORT COMMUNICATION. Electron Capture, Collision-Induced, and Electron Capture-Collision Induced Dissociation in Q-TOF
B American Society for Mass Spectrometry, 2011 J. Am. Soc. Mass Spectrom. (2011) 22:607Y611 DOI: 10.1007/s13361-010-0072-x SHORT COMMUNICATION Electron Capture, Collision-Induced, and Electron Capture-Collision
Applications of Tandem Mass Spectrometry: From Structural Analysis to Fundamental Studies
Applications of Tandem Mass Spectrometry: From Structural Analysis to Fundamental Studies Paulo J. Amorim Madeira and M. Helena Florêncio Centro de Química e Bioquímica, Departamento de Química e Bioquímica,
Mass Spectrometry of Proteins
University of South Bohemia Faculty of Science Mass Spectrometry of Proteins Biological Chemistry Bachelor Thesis Author: Tamara Buchberger Supervisor: Prof. RNDr. Libor Grubhoffer, CSc. May, 2011 Annotation
BIOINF 4399B Computational Proteomics and Metabolomics
BIOINF 4399B Computational Proteomics and Metabolomics Sven Nahnsen WS 13/14 3. Chromatography and mass spectrometry Overview Recall last lecture Basics of liquid chromatography Algorithms to predict and
Table of Contents... XI
Table of Contents Table of Contents... XI 1 Introduction...1 Learning Objectives...1 1.1 Aims and Scope...3 1.1.1 Filling the Black Box...5 1.2 What Is Mass Spectrometry?...5 1.2.1 Mass Spectrometry...6
Powerful Scan Modes of QTRAP System Technology
Powerful Scan Modes of QTRAP System Technology Unique Hybrid Triple Quadrupole Linear Ion Trap Technology Provides Powerful Workflows to Answer Complex Questions with No Compromises While there are many
Mass spectrometry has been used a lot in biology since the late 1950 s. However it really came into play in the late 1980 s once methods were
Mass spectrometry has been used a lot in biology since the late 1950 s. However it really came into play in the late 1980 s once methods were developed to allow the analysis of large intact (bigger than
Improvements in Electrospray Ionization Source Design and Advances in. Tandem Mass Spectrometry. Jared M. Bushey
Improvements in Electrospray Ionization Source Design and Advances in Tandem Mass Spectrometry Jared M. Bushey A dissertation submitted to the faculty of the University of North Carolina at Chapel Hill
WADA Technical Document TD2015IDCR
MINIMUM CRITERIA FOR CHROMATOGRAPHIC-MASS SPECTROMETRIC CONFIRMATION OF THE IDENTITY OF ANALYTES FOR DOPING CONTROL PURPOSES. The ability of a method to identify an analyte is a function of the entire
Tandem MS = MS / MS. ESI-MS give information on the mass of a molecule but none on the structure
Tandem MS = MS / MS ESI-MS give information on the mass of a molecule but none on the structure In tandem MS (MSMS) (pseudo-)molecular ions are selected in MS1 and fragmented by collision with gas. collision
Mass Spectrometry Course
Mass Spectrometry Course Árpád Somogyi Mass Spectrometry Laboratory, Department of Chemistry and Biochemistry University of Arizona, Tucson, AZ Eötvös University, Budapest April 11-20, 2012 1 2 UA Chemistry
1. Prepare the MALDI sample plate by spotting an angiotensin standard and the test sample(s).
Analysis of a Peptide Sequence from a Proteolytic Digest by MALDI-TOF Post-Source Decay (PSD) and Collision-Induced Dissociation (CID) Standard Operating Procedure Purpose: The following procedure may
Study of Non-Covalent Complexes by ESI-MS. By Quinn Tays
Study of Non-Covalent Complexes by ESI-MS By Quinn Tays History Overview Background Electrospray Ionization How it is used in study of noncovalent interactions Uses of the Technique Types of molecules
Introduction to LC-MS
Wednesday April 5, 2017 10am Introduction to LC-MS Amy Patton, MS Laboratory Manager, Pinpoint Testing, LLC Little Rock, AR DESCRIPTION: Amy Patton, laboratory manager for Pinpoint Testing, will begin
Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS)
Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) The first steps along the way to FT- ICR-MS date back to the 1930s, when Ernest Lawrence, at the University of California - Berkeley,
Propose a structure for an alcohol, C4H10O, that has the following
Propose a structure for an alcohol, C4H10O, that has the following 13CNMR spectral data: Broadband _ decoupled 13CNMR: 19.0, 31.7, 69.5 б DEPT _90: 31.7 б DEPT _ 135: positive peak at 19.0 & 31.7 б, negative
BST 226 Statistical Methods for Bioinformatics David M. Rocke. January 22, 2014 BST 226 Statistical Methods for Bioinformatics 1
BST 226 Statistical Methods for Bioinformatics David M. Rocke January 22, 2014 BST 226 Statistical Methods for Bioinformatics 1 Mass Spectrometry Mass spectrometry (mass spec, MS) comprises a set of instrumental
Università degli Studi di Bari CHIMICA ANALITICA STRUMENTALE
Università degli Studi di Bari Corso di Laurea Magistrale in Scienze Chimiche Corso di CHIMICA ANALITICA STRUMENTALE II semestre, 5 + 1 crediti (40 + 15 ore) Prof. Ilario Losito E-mail: illosdid@hotmail.com
LC-MS Based Metabolomics
LC-MS Based Metabolomics Analysing the METABOLOME 1. Metabolite Extraction 2. Metabolite detection (with or without separation) 3. Data analysis Metabolite Detection GC-MS: Naturally volatile or made volatile
MS-based proteomics to investigate proteins and their modifications
MS-based proteomics to investigate proteins and their modifications Francis Impens VIB Proteomics Core October th 217 Overview Mass spectrometry-based proteomics: general workflow Identification of protein
Selecting and planning for the right mass spectrometer
Selecting and planning for the right mass spectrometer MSACL US 2018 Palm Springs, CA Wednesday, January 24th Deborah French Ph.D., DABCC, FACB University of California San Francisco Learning Objectives
The introduction of electron capture dissociation
Characterization of Amino Acid Side Chain Losses in Electron Capture Dissociation Helen J. Cooper, Robert R. Hudgins, Kristina Håkansson, and Alan G. Marshall * Ion Cyclotron Resonance Program, National
Choosing the metabolomics platform
GBS 748 Choosing the metabolomics platform Stephen Barnes, PhD 4 7117; sbarnes@uab.edu So, I have my samples what s next? You ve collected your samples and you may have extracted them Protein precipitation
Instrumental Analysis. Mass Spectrometry. Lecturer:! Somsak Sirichai
303351 Instrumental Analysis Mass Spectrometry Lecturer:! Somsak Sirichai Mass Spectrometry What is Mass spectrometry (MS)? An analytic method that employs ionization and mass analysis of compounds in
Other Methods for Generating Ions 1. MALDI matrix assisted laser desorption ionization MS 2. Spray ionization techniques 3. Fast atom bombardment 4.
Other Methods for Generating Ions 1. MALDI matrix assisted laser desorption ionization MS 2. Spray ionization techniques 3. Fast atom bombardment 4. Field Desorption 5. MS MS techniques Matrix assisted
Profiling of Diferulates (Plant Cell Wall Cross- Linkers) Using Ultrahigh-performance Liquid. Chromatography-Tandem Mass Spectrometry
Supporting Information for: Profiling of Diferulates (Plant Cell Wall Cross- Linkers) Using Ultrahigh-performance Liquid Chromatography-Tandem Mass Spectrometry Ramin Vismeh a,b, Fachuang Lu c,d, Shishir
Mass Analyzers. Principles of the three most common types magnetic sector, quadrupole and time of flight - will be discussed herein.
Mass Analyzers After the production of ions in ion sources, the next critical step in mass spectrometry is to separate these gas phase ions according to their mass-to-charge ratio (m/z). Ions are extracted
20.2 Ion Sources. ions electrospray uses evaporation of a charged liquid stream to transfer high molecular mass compounds into the gas phase as MH n
20.2 Ion Sources electron ionization produces an M + ion and extensive fragmentation chemical ionization produces an M +, MH +, M +, or M - ion with minimal fragmentation MALDI uses laser ablation to transfer
PART I COPYRIGHTED MATERIAL INSTRUMENTATION INTRODUCTION
PART I INTRODUCTION INSTRUMENTATION The first part of this book is dedicated to a discussion of mass spectrometry (MS) instrumentation. We start with a list of basic definitions and explanations (Chapter
Lecture 8: Mass Spectrometry
intensity Lecture 8: Mass Spectrometry Relative abundance m/z 1 Ethylbenzene experiment CH 2 CH 3 + m/z = 106 CH 2 + m/z = 91 C 8 H 10 MW = 106 CH + m/z = 77 + 2 2 What information can we get from MS spectrum?
Chemistry 311: Topic 3 - Mass Spectrometry
Mass Spectroscopy: A technique used to measure the mass-to-charge ratio of molecules and atoms. Often characteristic ions produced by an induced unimolecular dissociation of a molecule are measured. These
WADA Technical Document TD2003IDCR
IDENTIFICATION CRITERIA FOR QUALITATIVE ASSAYS INCORPORATING CHROMATOGRAPHY AND MASS SPECTROMETRY The appropriate analytical characteristics must be documented for a particular assay. The Laboratory must
Lecture 8: Mass Spectrometry
intensity Lecture 8: Mass Spectrometry Relative abundance m/z 1 Ethylbenzene CH 2 CH 3 + m/z = 106 CH 2 + m/z = 91 C 8 H 10 MW = 106 CH + m/z = 77 + 2 2 What information can be obtained from a MS spectrum?
Protein analysis using mass spectrometry
Protein analysis using mass spectrometry Michael Stadlmeier 2017/12/18 Literature http://www.carellgroup.de/teaching/master 3 What is Proteomics? The proteome is: the entire set of proteins in a given
Computational Methods for Mass Spectrometry Proteomics
Computational Methods for Mass Spectrometry Proteomics Eidhammer, Ingvar ISBN-13: 9780470512975 Table of Contents Preface. Acknowledgements. 1 Protein, Proteome, and Proteomics. 1.1 Primary goals for studying
MASS SPECTROMETRY. Topics
MASS SPECTROMETRY MALDI-TOF AND ESI-MS Topics Principle of Mass Spectrometry MALDI-TOF Determination of Mw of Proteins Structural Information by MS: Primary Sequence of a Protein 1 A. Principles Ionization:
Dissociation of Even-Electron Ions
Dissociation of Even-Electron Ions Andrea Raffaelli CNR Istituto di Fisiologia Clinica Via Moruzzi, 1, 56124 Pisa. E-Mail: andrea.raffaelli@cnr.it Web: http://raffaelli.ifc.cnr.it A Simple? ESI Spectrum
SPECTRA LIBRARY ASSISTED DE NOVO PEPTIDE SEQUENCING FOR HCD AND ETD SPECTRA PAIRS
SPECTRA LIBRARY ASSISTED DE NOVO PEPTIDE SEQUENCING FOR HCD AND ETD SPECTRA PAIRS 1 Yan Yan Department of Computer Science University of Western Ontario, Canada OUTLINE Background Tandem mass spectrometry
(Bio)chemical Proteomics. Alex Kentsis October, 2013
(Bio)chemical Proteomics Alex Kentsis October, 2013 http://alexkentsis.net A brief history of chemical proteomics 1907: Eduard Buchner, demonstration of cell-free alcohol fermentation (i.e. enzymes) 1946:
VIII. QUADRUPOLE ION TRAPS: AN ION TRAPPING INST. A. BASIC DESIGN (REF: JMS, 1997, 32, 351)
VIII. QUADRUPOLE ION TRAPS: AN ION TRAPPING INST. A. BASIC DESIGN (REF: JMS, 1997, 32, 351) The trap consists of a ring electrode (internal radius is r 0 ) and two end caps separated by z 0.; all internal
Tandem mass spectrometry (MS/MS) is an essential
FOCUS: COOKS, 2006 DISTINGUISHED CONTRIBUTION IN MASS SPECTROMETRY AWARDEE ACCOUNT AND PERSPECTIVE Surface-Induced Dissociation of Small Molecules, Peptides, and Non-Covalent Protein Complexes Vicki H.
PC 235: Mass Spectrometry and Proteomics
PC 235: Mass Spectrometry and Proteomics Lecture 1 May 11 th, 2009 Shenheng Guan NIH NCRR Mass Spectrometry Facility, UCSF sguan@cgl.ucsf.edu Course Outline Lectures: 10hrs: 10am-12 in Genentech Hall Room
MS Goals and Applications. MS Goals and Applications
MS Goals and Applications 3 Several variations on a theme, three common steps Form gas-phase ions choice of ionization method depends on sample identity and information required Separate ions on basis
Mass Spectrometry. Electron Ionization and Chemical Ionization
Mass Spectrometry Electron Ionization and Chemical Ionization Mass Spectrometer All Instruments Have: 1. Sample Inlet 2. Ion Source 3. Mass Analyzer 4. Detector 5. Data System http://www.asms.org Ionization
Quadrupole Storage Mass Spectrometry
Quadrupole Storage Mass Spectrometry RAYMOND E. MARCH AND RICHARD J. HUGHES Trent University Peterborough, Ontario, Canada with a historical review by John F. J. Tbdd University of Kent Canterbury, Kent,
GRADUATE COURSE IN MASS SPECTROMETRY: LECTURE 2
DEPARTMENTS OF CHEMISTRY AND BIOCHEMISTRY GRADUATE COURSE IN MASS SPECTROMETRY: LECTURE 2 Mass Analysers Shabaz Mohammed October 20, 2015 High Vacuum System Turbo pumps Diffusion pumps Rough pumps Rotary
ion mobility spectrometry IR spectroscopy
Debasmita Gho 29.10.2016 Introducti on Owing to its accuracy, sensitivity, and speed, mass spectrometry (MS) coupled to fragmentation techniques is the method of choice for determining the primary structure
Protein Quantitation II: Multiple Reaction Monitoring. Kelly Ruggles New York University
Protein Quantitation II: Multiple Reaction Monitoring Kelly Ruggles kelly@fenyolab.org New York University Traditional Affinity-based proteomics Use antibodies to quantify proteins Western Blot Immunohistochemistry
Chapter 5. Complexation of Tholins by 18-crown-6:
5-1 Chapter 5. Complexation of Tholins by 18-crown-6: Identification of Primary Amines 5.1. Introduction Electrospray ionization (ESI) is an excellent technique for the ionization of complex mixtures,
INTRODUCTION TO MASS SPECTROMETRY Instrumentation, Applications and Strategies for Data Interpretation
INTRODUCTION TO MASS SPECTROMETRY Instrumentation, Applications and Strategies for Data Interpretation FOURTH EDITION J. THROCK WATSON 0. DAVID SPARKMAN 2007 John Wiley & Sons Ltd, Chichester, 978-0-470-51634-8
CHROMATOGRAPHY AND MASS SPECTROMETER
22 CHROMATOGRAPHY AND MASS SPECTROMETER 22.1 INTRODUCTION We know that the biochemistry or biological chemistry deals with the study of molecules present in organisms. These molecules are called as biomolecules
Multi-stage Mass Spectrometry up to MS 4 on a QTof System
Valerie Koppen, 1 Russell Mortishire-Smith, 2 Filip Cuyckens 1 1 Janssen R&D, Beerse, Belgium 2 Waters Corporation, Wilmslow, United Kingdom APPLICATION BENEFITS This application note illustrates how multi-stage
Mass Spectrometry in MCAL
Mass Spectrometry in MCAL Two systems: GC-MS, LC-MS GC seperates small, volatile, non-polar material MS is detection devise (Agilent 320-MS TQ Mass Spectrometer) Full scan monitoring SIM single ion monitoring
Instrumentation development for coupling ion/ion reactions and ion mobility in biological mass spectrometry
Graduate Theses and Dissertations Iowa State University Capstones, Theses and Dissertations 2008 Instrumentation development for coupling ion/ion reactions and ion mobility in biological mass spectrometry
L.7. Mass Spectrum Interpretation
L.7. Mass Spectrum Interpretation Fragmentation reactions Spectrum interpretation Confirmation of ion structural assignment Biomolecule dissociation Fragmentation reactions 1. Fragmentation reactions of
Thermo Scientific LTQ Orbitrap Velos Hybrid FT Mass Spectrometer
IET International Equipment Trading Ltd. www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Thermo Scientific LTQ Orbitrap Velos
Oligonucleotide ions can be formed using different
Identification of Fragmentation Channels of Dinucleotides Using Deuterium Labeling Dorothée Balbeur, a Dominique Dehareng, b and Edwin De Pauw a a Laboratory of Mass Spectrometry, University of Liège,
The development of environmentally-benign herbicides
Studies of Rearrangement Reactions of Protonated and Lithium Cationized 2-Pyrimidinyloxy-N-Arylbenzylamine Derivatives by MALDI-FT-ICR Mass Spectrometry Hao-Yang Wang and Yin-Long Guo Shanghai Mass Spectrometry
Sponsored document from Journal of the American Society for Mass Spectrometry
Sponsored document from Journal of the American Society for Mass Spectrometry Activated Ion Electron Capture Dissociation (AI ECD) of Proteins: Synchronization of Infrared and Electron Irradiation with
The 4-Source Ion Trap
Introduction ur research program is devoted to the study of gaseous macro-ions applied within the context of mass spectrometry to challenging analysis prolems. ur work involves instrument development,
Mass spectrometry gas phase transfer and instrumentation
Objectives of the Lecture spectrometry gas phase transfer and instrumentation Matt Renfrow January 15, 2014 1. Make ions 2. Separate/Analyze 3. Detect ions 4. What is mass resolution and mass accuracy?
Lecture 15: Introduction to mass spectrometry-i
Lecture 15: Introduction to mass spectrometry-i Mass spectrometry (MS) is an analytical technique that measures the mass/charge ratio of charged particles in vacuum. Mass spectrometry can determine masse/charge
Technical Note. Introduction
Technical Note Analysis and Characterization of Psilocybin and Psilocin Using Liquid Chromatography - Electrospray Ionization Mass Spectrometry (LC-ESI-MS) with Collision-Induced-Dissociation (CID) and
Chapter 4. Rapid Comm. Mass Spectrom. 2006; 20: The Netherlands
Combined infrared multiphoton dissociation and electron-capture dissociation using co-linear and overlapping beams in Fourier transform ion cyclotron resonance mass spectrometry Romulus Mihalca 1, Yuri
Bogdan A. Budnik and Parminder Kaur Cardiovascular Proteomics Center, Boston University School of Medicine, Boston, Massachusetts, USA
Characterization of a New qqq-fticr Mass Spectrometer for Post-Translational Modification Analysis and Top-Down Tandem Mass Spectrometry of Whole Proteins Judith A. Jebanathirajah and Jason L. Pittman
This is the total charge on an ion divided by the elementary charge (e).
12.2 Fundamentals and general terms Accelerator mass spectrometry An ultra-sensitive technique using tandem accelerators employed mainly for the study of long-lived radioisotopes, and stable isotopes at
FOCUS: MOBILE PROTON MODEL
FOCUS: MOBILE PROTON MODEL Separation and Identification of Structural Isomers by Quadrupole Collision-Induced Dissociation-Hydrogen/Deuterium Exchange-Infrared Multiphoton Dissociation (QCID-HDX-IRMPD)
Molecular Mass Spectrometry
Molecular Mass Spectrometry Mass Spectrometry: capable of providing information about (1) Elemental composition of samples of matter: atomic mass (2) Structures of inorganic, organic, and biological molecules
Relaxation of energized PAHs
Relaxation of energized PAHs 2018 1 st year PhD student Supervisor: Dr. Christine Joblin May 16, 2018 Mid-Infrared Spectra Emission features 3.3, 6.2, 7.7, 8.6, 11.2, 12.7 µm Vibrational modes of PAHs
New Approaches in the Analysis of Amadori Compounds
7 TH Wartburg Symposium on Flavor Chemistry and Biology Eisenach, April 21 st - April 23 rd, 2004 New Approaches in the Analysis of Amadori Compounds T. Davidek, I. Blank, K. Kraehenhuehl, J. Hau and S.
高等食品分析 (Advanced Food Analysis) I. SPECTROSCOPIC METHODS *Instrumental methods: 1. Spectroscopic methods (spectroscopy): a) Electromagnetic radiation
*Instrumental methods: 1. Spectroscopic methods (spectroscopy): a) Electromagnetic radiation (EMR): γ-ray emission X-Ray absorption, emission, fluorescence and diffraction Vacuum ultraviolet (UV) absorption
Anion-Peptide Adduct Formation and Decomposition As Studied by Fourier Transform Ion Cyclotron Resonance (FT-ICR) Mass Spectrometry
University of New Orleans ScholarWorks@UNO University of New Orleans Theses and Dissertations Dissertations and Theses Fall 12-20-2013 Anion-Peptide Adduct Formation and Decomposition As Studied by Fourier
Supporting Information - Polymer analysis in the second dimension: preliminary studies for the characterisation of polymers with 2D MS
Supporting Information - Polymer analysis in the second dimension: preliminary studies for the characterisation of polymers with 2D MS Federico Floris, 1 Claudio Vallotto, 1 Lionel Chiron, 2 Alice M. Lynch,
Recent trends in mass spectrometer development
Anal Bioanal Chem (2004) 378 : 845 850 DOI 10.1007/s00216-003-2287-1 REVIEW James W. Hager Recent trends in mass spectrometer development Received: 25 July 2003 / Revised: 8 September 2003 / Accepted:
Interpretation of Organic Spectra. Chem 4361/8361
Interpretation of Organic Spectra Chem 4361/8361 Characteristics of Common Spectrometric Methods H-1 C-13 MS IR/RAMAN UV-VIS ORD/CD X- RAY Radiation type RF RF Not relevant IR UV to visible UV to visible
Mini review for final
Mini review for final NB: This does not cover all material! (very) Rough outline Units, Conversions, Powers of 10, Prefixes Errors: Sig Figs, Error propagation Statistics: Normal distribution, t-test,
Protein Quantitation II: Multiple Reaction Monitoring. Kelly Ruggles New York University
Protein Quantitation II: Multiple Reaction Monitoring Kelly Ruggles kelly@fenyolab.org New York University Traditional Affinity-based proteomics Use antibodies to quantify proteins Western Blot RPPA Immunohistochemistry
Electrospray ionization mass spectrometry (ESI-
Automated Charge State Determination of Complex Isotope-Resolved Mass Spectra by Peak-Target Fourier Transform Li Chen a and Yee Leng Yap b a Bioinformatics Institute, 30 Biopolis Street, Singapore b Davos
Proteomics. November 13, 2007
Proteomics November 13, 2007 Acknowledgement Slides presented here have been borrowed from presentations by : Dr. Mark A. Knepper (LKEM, NHLBI, NIH) Dr. Nathan Edwards (Center for Bioinformatics and Computational
Analysis of chemically modified proteins by FT-ICR MS
Analysis of chemically modified proteins by FT-ICR MS PETR NOVAK EU FT-ICR MS End User School 1 UEF Chemistry August 19-24 2018 Joensuu, Finland Structural Mass Spectrometry Disulfide bonds mapping Limited
FOCUS: VAN BERKEL, 2005 BIEMANN MEDAL AWARDEE Determination of Cooling Rates in a Quadrupole Ion Trap
FOCUS: VAN BERKEL, 2005 BIEMANN MEDAL AWARDEE Determination of Cooling Rates in a Quadrupole Ion Trap David M. Black, Anne H. Payne, and Gary L. Glish Department of Chemistry, University of North Carolina