Mass Spectrometry and Proteomics - Lecture 2 - Matthias Trost Newcastle University

Transcription

1 Mass Spectrometry and Proteomics - Lecture 2 - Matthias Trost Newcastle University matthias.trost@ncl.ac.uk

2 Previously: Resolution and other basics MALDI Electrospray 40

3 Lecture 2 Mass analysers Detectors Tandem mass spectrometry 41

4 Mass analysers Sector instruments (magnetic- B; electrostatic E) Time-of-Flight (TOF) Quadrupol (Q) Ion Trap (IT) Ion Cyclotron Resonance (ICR) Orbitrap 42

5 Factors affecting choice of Mass analyser Mass range Resolving power Mass accuracy and ease of calibration Speed Scanning or not scanning? Ease of interfacing with ion sources Ease of use for tandem mass spectrometry 43

6 Sector instruments Mass range up to m/z 15,000 Resolving power up to 100,000 Most suitable ionisation: EI, CI, FAB, FD Less suitable for ESI or MALDI Rarely used nowadays, large, complicated 44

7 Time-of-Flight (TOF) Requires pulsed ion packages (MALDI, pulser in ESI-QTOF) Ions travel in a field-free region according to their kinetic energy E k =mv 2 /2; i.e. mass can be determined if speed is measured. Theoretically unlimited mass range (realistically >250k) Resolving power up to ~30,000 Electrostatic mirrors (Reflectrons) used to lengthen flight time and reduce spread of ion populations higher resolution Fast, sensitive, simple, cheap 45

8 Better resolving power: Reflectron TOF Acceleration region Reflectron Ion source Detector Field-free drift region Ion source Reflectron Detector Reflectron 46

9 Time-of-Flight (TOF) Agilent 47

10 Quadrupole (Q) Four parallel metal rods, opposite rods have DC potentials (U) on which a radio frequency is superimposed. Ions oscillate and at a fixed U/R f only a certain m/z passes through the quadrupole. By continuously varying U/R f, the Q can be used for scanning. Ion motion in a Quadrupole Quadrupole schematic 48

11 Quadrupole (Q) Advantages: small, light, portable; robust; no high voltage; high dynamic range; cheap Disadvantages: low resolution (~2000); mass range m/z <3,000 49

12 Miniature Mass Spectrometers 50

13 Ion Trap (IT) Two commonly used forms exist: Linear Ion Trap (LIT e.g. Velos, Thermo) and 3D or Paul Ion Trap (QIT e.g. amazon, Bruker) Similar to quadrupole but in 3D. Equations of ion motion are complex. Space charge effects possible when many ions in trap. Resolution 2,000-10,000 depending on scanning speed. Mass range <2,000, can only scan down to 1/3 m/z of precursor in MS/MS loss of low molecular masses. Linear IT 51

14 3D Ion Trap (QIT) 3D IT 52

15 Potential well and ion motion in 3D trap 53

16 Fourier Transform Ion Cyclotron Resonance (FT ICR) The m/z is determined based on the cyclotron frequency of ions in a fixed magnetic field (requiring superconducting magnet). Ions are trapped in a Penning Trap (ion trap in magnetic field) and excited to a cyclotron motion by an oscillating electric field. Very high mass accuracy (<0.5 ppm) and very high resolution (>1M) Ions are not destroyed thus time dependent studies such as gas reactions in the ICR cell are possible. Very expensive in purchase and maintenance, tricky to run 54

17 FT-ICR MS working principle s/theory/fticr-massspec.html Cyclotron frequency of ion: ω C zb 2 m 55

18 Orbitrap Developed in 2000 by Alexander Makarov, commercialised in 2005 by Thermo. Ions are injected into the electric field between electrodes and trapped. Ions oscillate around and along the inner electrode according to the m/z. FT allows measurement of mass. Requires ultra-high vacuum (10-10 bar) Requires C-trap to inject tight ion packages. High mass accuracy (<1 ppm), high resolution (<500k) Sensitive and high dynamic range. Much cheaper than FT ICR. 56

19 The Story of the Orbitrap Alexander Makarov with Orbitrap 2013 Alexander Makarov, 1990s The Analytical Scientist, Nov

20 Ion Mobility Spectrometer Ions are separated by shape, not m/z. Drift gas and electric field opposed or orthogonal. Drift time increases with collisional cross-section. Often combined with mass spectrometry. 58

21 Ion mobility spectrum Oligomers of Alzheimer s disease Aβ1-40 form several different structural states. Kloniecki et al,

22 Detectors Commonly used are Secondary Electron multipliers (SEM), Channel electron multipliers (CEM) and Microchannel Plates (MCP). These work by the principle that energetic particles hitting a semiconductor or metal surface lead to emission of secondary electrons. These can than be used to further amplify the signal in a cascade. Gain of Detectors age and require regular replacement. 60

23 Detectors: MCP Microchannel Plates (MCP) Note: ion counting detectors also give signals of impact of energetic neutrals, electrons and photons. Therefore, care has to be taken, not to allow other particles than the massanalysed ions to hit the detector. 61

24 Tandem mass spectrometry MS/MS, MS 2 or tandem mass spectrometry Tandem-in-space can be performed on (ion beamtransmitting) Hybrid instruments with 2 distinct mass analysers. Tandem-in-time in Ion traps: ion selection, activation and product ion analysis in the very same place. 62

25 Product Ion Scan 1. Precursor or Parent Ions are selected and isolated 2. Fragmentation by CID 3. Product or Daughter Ions are characterised by Q3 4. Most common MS/MS experiment used for protein ID 63

26 Precursor Ion Scan 1. Product Ion is selected and Q3 is parked 2. Q1 scans normally 3. Only precursors which fragment to produce a selected product ion are detected. 4. Often used to target peptides with specific modifications resulting in reporter ions. 64

27 Neutral Loss Scan 1. The mass of a functional group whose loss is to be detected is selected 2. Both Q1 and Q3 scan simultaneously, offset by the selected neutral loss mass 3. Fragmentation by CID 4. Product ions are detected only when specified loss occurs in Q2, indicating the presence of the moiety of interest. 5. Often used for peptide modifications such as phosphorylation. 65

28 Selected Reaction Monitoring (SRM) 1. Precursor and product ion mass need to be known. 2. Q1 is fixed on specified mass. 3. Fragmentation by CID. 4. Q3 is fixed on specified fragment of mass selected in Q1. 5. Very commonly used for accurate peptide/small molecule quantitation. 66