Talk Line-up. MassTech, Inc Columbia Gateway Drive Columbia, Maryland (443) Goals for MT Explorer 50 Development

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1 A Field Deployable Ion Trap Mass Spectrometer with Atmospheric Pressure Interface Arnold Lee *, Alexander Misharin, Konstantin Novoselov, Victor Laiko, Vladimir M. Doroshenko Talk Line-up Goals for MT Explorer development MTE design features MTE specifications MTE configurations MTE potential market MassTech, Inc. 699 Columbia Gateway Drive Columbia, Maryland 6 () Goals for MT Explorer Development MTE Design Features Field-deployable instrument for: MS Detector small molecule analysis biomolecule analysis Sensitivity comparable with that of commercial desktops Ion Source Ion Guide Optics Mass Analyzer Ion Detection System Instrument Control and Data Acquisition Laptop or Personal Computer Interfacing with all atmospheric pressure (AP) ionization Turbo Pump Turbo Pump Diaphragm Pump techniques Providing software tools for custom application software development Two vacuum chamber design (U.S. Patent 8,7,99) Bounded hydrogen (metal hydride) cartridge as a source for buffer gas (U.S. Patent 8,76,86)

2 y y y y9 9. y y. 77. y MTE Design Features MTE: Specifications Atmospheric pressure interface (API) MS and MS/MS modes of operation Mass range: -, Da Mass accuracy. Da Weight 7 lb Dimensions x7 x Power -W ESI-MS of the calibration mixture A. Cone, heating elements and inlet capillary APCI-MS/MS of TNT p ( ) B. Ion optics: inlet hexapole ion guide and conductance limit orifice C. Ion Optics: MS analyzer hexapole ion guide D. Ion trap mass analyzer Intensity ( au) E. Conversion dynode and electron multiplier F. Pre-amplifier Ionization: APCl Polarity: Negative pg Loaded MTE Interfaced with AP-MALDI Ion Source MTE Interfaced with AP-MALDI Ion Source Limit of detection MS/MS capability. fmol fmol 8 Relative Inten sity (%) y y y6 y7 y8 y9 y y y y AP MALDI MS/MS spectra of PR peptide ions: fmol and fmol (inse rt) loaded AP MALDI MS spectra of peptide mixture of Angiotensin II (MW 6 Da) and PR (MW Da): fmol and fmol (insert) loaded 7 8

3 MTE Interfaced with AP-MALDI Ion Source MTE Interfaced with DART Ion Source Identification of Bacillus Globigii spores * 97.6* Detection of narcotics *.7 AP MALDI MS spectrum of Bacillus Globigii spores after chemical processing and digestion by trypsin (BG spores biomarker peptide are indicated) / 8 6 ng.8 / AP MALDI MS/MS spectrum of BG peptide ion with value of 8 in the spectrum above 9 DART MS and DART MS/MS spectra of Cocaine. The molecular ion is indic ated MTE Interfaced with DART Ion Source MTE Interfaced with DART Ion Source Parathion ng DART MS and DART MS/MS spectra of Parathion. The molecular ion is indic ated Carbofuran ng DART MS and DART MS/MS spectra of Carbofuran. The molecular ion is indic ated

4 MTE Interfaced with DS-APCI Ion Source MTE Interfaced with DS-APCI Ion Source Detection of narcotics / ng DS-APCI MS (above) and MS/MS (below) spectra of Cocaine. The molecular ion is indic ated Parathion Relative Intensity pg in use pg in use DS-APCI MS and MS/MS spectra of Parathion. The molecular ion is indic ated MTE Interfaced with DS-APCI Ion Source MTE Interfaced with DS-sESI Ion Source Detection of explosives Methyl Salicylate ng in use DS-APCI MS and MS/MS spectra of Methyl Salicylate. The molecular ion is indic ated / / DS-sESI MS (above) and MS/MS (below) spectra of TNT. Direct injection. The molecular ion is indicated pg in use,.% formic acid Solid probe of pg of TNT; MS mode;.% formic acid. 6

5 MTE Interfaced with DS-sESI Ion Source MODAS: Control Software Detection of explosives DS-sESI MS (above) and MS/MS spectra (below) of ng RDX by direct injection. The molecular ion is indicated.% formic acid. Software tools for custom application software development Normal and expert mode of operations Built-in chromatogram viewer DS-sESI MS spectra of ng PETN (above)..% formic acid. 7 8 Portable MS: What is the potential market? Field-deployable mass spectrometry (MS) applications Portable MS applications Environmental MS applications Ambient MS applications Fieldable biological MS applications (like DoD, DHS, DARPA) 9

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