Karyotype Ana lysis and Cytology O bserva tion on M e iosis of Paeon ia ostii Phoen ix W h ite

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1 2007, 34 (2) : Acta Horticulturae Sinica 1, 2, 2, 3, 23, 1, 2 ( 1, ; ) 2, ; 3, : :, 2n = 10, : 2n = 2x = 10 = 6m + 2 sm + 2 st, 2A,, :,, 2 3 : ; ; ; ; : S : A : X (2007) Karyotype Ana lysis and Cytology O bserva tion on M e iosis of Paeon ia ostii Phoen ix W h ite L I Zi2feng 1, 2, WANG J ia 2, 3, HU Yong2hong 23, L IU Q ing2hua 1, and Q IN Jun 2 ( 1 College of L andscape A rchitecture and A rt, Laiyang A gricultural U niversity, Q ingdao, Shandong , China; B otanical Garden, Shanghai , China; 3 College of Landscape, B eijing Forestry U niversity, B eijing , China) 2 Shanghai Abstract: The karyotype and meiosis course of pollen mother cell on Paeonia ostii Phoenix W hite of cultivar group of tree peony from South Yangtse R iver of China have been observed and researched. Results show that the chromosome number of Paeonia ostii Phoenix W hite is 10. It is lip loid. Kitagawa is 2n = 2x = 10 = 6m + 2 sm + 2 st, which is belong to 2A type. It has not observed chromosome satellites. Extrapolated Paeonia ostii Phoenix W hite with Paeonia ostii is not the different same thing, whether there is mainly dis2 p lays in chromosome satellites. Compare with the karyotype of Paeon ia ostii and show that from Paeonia ostii to Paeonia ostii Phoenix W hite evolved the course from pure to m ixed and shut different grade. Meiosis is out of step. It was observed that the chromosome bridge was in anaphase, telophase and the diagonal bridge, sp lits unequally, one pair of bridges, etc. were in anaphase, telophase. The unusual phenome2 non in meiosis course of pollen mother cell of Paeonia ostii PhoenixW hite have been analyzed from cell sci2 ence of heredity level. beginning of M arch in Shanghai. The best time to observation of the pollen master cell meiosis is end of February to the Key words: Paeon ia ostii; Chromosome; Karyotype; Pollen mother cell; M eiosis (Cultivar Group of Tree Peony from South Yangtse R iver of China) : ; : : ( F050304) 3 Author for correspondence ( E2mail: huyonghong68@hotmail1com)

2 412 34, ( Paeon ia ostii T. Hong et J. X. Zhang) (, 1999;, 2002),,,,,,, (, 2005), ;,,,, ( Paeonia ostii T. Hong et J. X. Zhang Phoenix W hite ) ( ), 4 ( ) cm, 9 11 ( = 1 3) 24 h; 50% ; 1 mol L - 1 HCl m in;, 1 2 mm, ;, 5 (, 1985) ( Stebbins, 1971) ( cm ), ( ) 24 h, 70% ( 1 mol L - 1 HCl 3 m in),,, 1 2,,,, (, , (, 2003) (,, : 20% 1996), 9 11) : 2n = 2x = 10 = 6m + 2 sm + 2 st m, 2 (012), 115 ( < 2 1), 2A, Table 1 Karyotype ana lysis of Paeon ia ostii Phoen ix W h ite No. + = L + S = Total ( / ) A rm ratio (L /S) Type = m = m = m = sm = st

3 2 : F ig. 1 M etapha se of Paeon ia ostii Phoen ix W h ite soma tic cell , (, 1 15) 82, 1813%, 418% ; 2312%,, 7132% ; 2143%, ;, 5%, 3011% 1 5, (, 11) (, a n) 128, 1, 6125%,,, 25%,, 3512%,, 2917%,, 3 311, (2n = 2x = 10 = 8m + 2 st) (, 2003) : [ 2n = 2x = 10 = 6m + 2 sm (1SAT) + 2 st (1SAT) ],,,, P1ostii ( ) (, 1998, 2005) ;

4 (, 2001) (1SAT) : 2n = 2x = 10 = 6m ( 3SAT) + 2 sm + 2 st (2SAT), SAT SAT -, SAT 1 /2,, ; SAT,, :,,, : m 3 /5, 2A,, ( Stebbins, 1971), 312 cm ( ), , h 1,,,,,,, 1 2 :,, :,, ;, ( ), 5,,,,,, :,,,, 1, 2,,, ; 4,, 2 (, 2003;, 2004) 313,,,

2 : 415 : 1 151 1 a n1 2 11 ; 21 ; 31 ; 41 ; 51 ; 61 ; 7, 81 ; 9 111 ; 121 ; 131 ; 141 ; 151 a ; b1 ; c1 ; d1 ; e, j1 ; f, h1 ; g1 ; i1 ; k1 ; l1 ; m1 ; n1 Explana tion of pla tes: 1-151 The first

5 2 : 415 : a n ; 21 ; 31 ; 41 ; 51 ; 61 ; 7, 81 ; ; 121 ; 131 ; 141 ; 151 a ; b1 ; c1 ; d1 ; e, j1 ; f, h1 ; g1 ; i1 ; k1 ; l1 ; m1 ; n1 Explana tion of pla tes: The first meiosis; a - n. The second meiosis. 11Leptotense; 21Zygotene; 31Pachgtene; 41D iplotene; 51D iakinesis; 61Metaphase ; 7, 81Chromosomes disjunction ahead of time; 9-111Anaphase ; 121Chromosome bridge in anaphase ; 131Telophase ; 141Chromosome bridge in Telophase ; 151D iad. a1prophase ; b1metaphase ; c1 Initial stage of Anaphase ; d1chromosomes diagonal bridge in Anaphase ; e, j1anaphase ; f, h1unequal separation of chromosome in Metaphase ; g1chromosome double ridges inmeta2 phase ; i1chromosomes diagonal bridge in Anaphase ; k1 Telophase ; l1chromosome bridge in Telophase ; m1tetrad; n. Tritrad.

6 References Chen Rui2yang, SongW en2qin, L i Xiu2lan, L imao2xue Chromosome atlas ofmajor econom ic plants genome in China III. Beijing: Science Press: ( in Chinese),,, ( 3 ). : : Chinese Peony Book Condification Comm ittee Chinese peony book. Beijing: China Science and Technology Publishing House: ( in Chinese) : : He L i2xia The cytogenetic observation on pollen mother cell of Paeonia lutea. Journal oflanzhou University (Natural Sciences), 6 (40) : ( in Chinese) ( ), 6 (40) : Hong De2yuan, Pan Kai2yu Taxonom ical history and revision of Paeonia sect. Moutan ( Paeoniaceae). Acta Phytotaxonom ica Sinica, 37 (4) : ( in Chinese), , 37 (4) : Hong De2yuan, Pan Kai2yu Make up of tha note revision of Paeonia sect. Moutan ( Paeoniaceae). Acta Phytotaxonom ica Sinica, 43 (3) : ( in Chinese), , 43 (3) : L i Jia2jue Peonies in Chinese horticulture. Beijing: China Forestry Publishing House: ( in Chinese) : : L i Jia2jue Pictorial record of Chinese tree peony varieties? Northwest, Southwest, South Yangtse river. Beijing: China Forestry Publishing House: 8-9. ( in Chinese) : : 8-9. L imao2xue, Chen Rui2yang A suggestion on the standardization of karyotype analysis in p lants. Journal ofw uhan Botanical Research, 3 (4) : ( in Chinese), , 3 (4) : L imao2xue, Zhang Zan2p ing Research techniques of crops chromosome. Beijing: China Agricultural Press: ( in Chinese), : : L i Xue, Chen L i2mei, Du Jie, L iangw an2fu, Xing Heng2tai Observation on abnormal meiosis of pollen mother cells in L ilium davidii var. unicolor. Acta Botanica Boreali2Occidentalia Sinica, 23 (10) : ( in Chinese),,,, , 23 (10) : Shen Bao2an Taxonom ic identification and revision ofmedicinal plants in sect. Moutan of the genus Paeonia. L ishizhenmedicine andmate2 ria Medica Research, 12 (4) : ( in Chinese) , 12 (4) : Stebbins G L Chromosomal evolution in higher plants. London: Edward A rnod L id.

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