Iden tif ica tion of the Aroma Com pounds in Seleng W orm wood by Ga s Chroma tography - O lfactom etry
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1 2009, 36 (11) : Acta Horticulturae Sinica,,,, 3 (, ) :, ( GC - O) ( GC - MS), 21,, , ( - ) - ( - ) - : ; ; ; : S 647 : A: X (2009) Iden tif ica tion of the Aroma Com pounds in Seleng W orm wood by Ga s Chroma tography - O lfactom etry XU Ying, N I Guang2yuan, CHEN Q ing2chan, SUN Zhi2da, and YANG Er2ning 3 ( College of Food Science and Technology, Huazhong A gricultural U niversity, W uhan , China) Abstract: The volatile compounds in seleng wormwood were extracted by using headspace solid phase m icro2extraction technology and analyzed by both gas chromatography2olfactom etry and gas chromatography2 mass spectrometry. There were totally twenty2one compounds identified. B ased on the odor intensity, the most important aroma compound in seleng wormwood was trans222hexenal, 2phellandrene, cis2ocimene, 32thu2 janone, camphor, cucum ber aldehyde, ( 2) 2borneol and ( 2) 2bornylacetate. Key words: seleng wormwood; aroma compound; solid phase m icro2extraction; gas chrom atography - olfactom etry (A rtem isia selengensis Turcz. ),,, (, 1975;, 1987;, 1995),, ( gas chromatography2mass spectrometry, GC - MS) (, 2002;, 2004;, 2007), ( gas chromatography2olfactometry, GC - O),,,, (Mayol & Acree, 2001), GC - O (, 2004;, 2004) ( headspace solid phase m icro2extraction, HS2SPME),, : ; : : (2006BAD27B09) 3 Author for correspondence ( E2mail: yangerning@mail1hzau1edu1cn)
2 11 : 1677, ,,,,,, , (DVB /CAR /PDMS 2 cm Supelco ) (250 ) 5 m in 50 g 250 ml,,,, 5 g 2 g 3 ml(40 ml ), /,, m in, GC - O GC - O Sniffer 9000 ( B rechb hler) 1 1,, ( F ID),,,, 6890 (HP25) :, 210 ml m in - 1 ; : 30 m 0132 mm, 0125 m; 40, 1 m in, 4 m in , 8 m in , 4 m in; 250, ; ( F ID ) 260, 30 ml m in - 1, 400 ml m in - 1, 25 ml m in m in, 3 ( 1 2) 30 h 3,, ( 9 ) 16, 0, 1, 7, 15 3,, GC - MS (5973 ) (HP25m s) : 0125 m;, 110 ml m in - 1 ; : 30 m 0125 mm, 40, 1 m in, 4 m in , 8 m in , 4 m in; 250, ; 280, E I70 ev, m /z (R I) C 5 C 20, ( t R ), ( t M ) = /, ( t R ) = t R - t M Kov ts (Van & Kratz, 1963) R I
3 GC - O 1, a GC - O, ( 1, b) 1, a b, GC - O F ID,, ( 2 15) ( 17), F ig. 1 ( a) ( b) 1 21, 1 1 The Chroma togram ( a) and the aroma2gram ( b) of the vola tiles in seleng worm wood Peak numbers 1 to 21 correspond to twenty2one aroma compounds been identified in seleng wormwood, whose chem ical names were listed in table GC - M S GC - MS, W iley, 1 (www1flavornet1com ), ( 10), 1 +, GC - O, 1 +, 28 GC - MS, 7, , 15 6GC - O,
4 11 : , - 2 -, ; - 3 -, ; - ( - ) - ; ( - ) -, 1, ( - ) - ( - ) - 8, Compounds Peak No. 1 Table 1 Iden tif ica tion of the aroma com pounds in seleng worm wood (R I) Retention index Measured value Reference value Identification R Iidentification O rdor O rdor description 1 Hexanal Grass Trans222Hexenal Green apple 3-2p inene Turpentine 4 4 Octanol Mushroom 4 5 2myrcene Musty 4 6 2phellandrene M int p2cymene Gasoline Trans2ocimene Herb Cis2ocimene Rose 6 10 ( + ) Earth 4 ( + ) 242carene thujanone M int 5 12 Camphor Camphor 5 13 Cucumber aldehyde Cucumber 6 14 Borneol Herb 4 15 ( - ) - ( - ) 2Borneol , 5 Hay, camphor 16 ( - ) , 5 ( - ) 2Bornylacetate Herb, grass 17 Caryophyllene , 3 Sweet, app le 18 Isocaryophillene Wood cubebene , 3 Mushroom, grass 20 Farnesyl acetate Dairy 3 21 Hep tadecatriene Balsam ic 4 O rdor intensity 3,,, GC - MS GC - O, (Jetti et al., 2007),,,, 7%,, (2002) 9,,
5 GC - MS47, ( 2007), - GC - MS, GC - O 21,,, , ( - ) - ( - ) -,,,,,,,,,, SPME SPME,,, References Beijing Institute of Botany, Chinese Academy of Sciences L llustrated handbook of Chinese higher p lants. Beijing: Science Press. ( in Chi2 nese) (). :. Chen Xin, Ye W en2feng, Sun L ing2feng Study on chem ical constituents of the volatile oil from stem s and leaves of artem isia selengensis. Physical Testing and Chemical Analysis Part B: Chem ical Analysis, 40 (11) : ( in Chinese),, , 40 (11) : Jetti R R, Yang E, Kurnianta A, Finn C, Q ianm C Quantification of selected aroma2active compounds in strawberries by headspace solid2 phase m icroextraction gas chromatography and correlation with sensory descriptive analysis. Food Science, 72 (7) : L i Bao2tong, Tang L i2mei, Yu Man2gen Studies on the chem ical components of essential oil from artem isia selengensis and its effect to the bioacitvity of helicoverpa arm igera. Cotton Science, (4) : ( in Chinese),, , (4) : L i Jing2m ing, Yan Hong, N i Yuan2ying, Cai Tong2yi The app lication of sniffing analysis to determ ination of aroma components in strawber2 ry juice. Food and Fermentation Industries, 30 (3) : 1-4. ( in Chinese),,, ( sniffing)., 30 (3) : 1-4. Mayol A R, Acree T E Advances in gas chromatography2olfactometry. Gas chromatography2olfactometry, W ashington D C: American Chem ical Society: Tian Huai2xiang, W ang Zhang, Xu Shi2ying Characterization of odor2active compounds in jinhua ham by GC - O lfactometry. Food and Fer2 mentation Industries, 30 (12) : ( in Chinese),, GC - O., 30 (12) : Tao Gui2quan Chinese map. Beijing: Chinese Peopleπs L iberation A rmy. ( in Chinese) :. van den Dool H, Kratz P D A generalization of the retention index system including linear temperature p rogrammed gas2liquid partition chromatography. Chromatography, 11: Xu Zhong2hai, W u Ying, L iu D i2gui, Chen Bo Study on chem ical constituents of the essential oil from artem isia selengensis in dongting lake area. Chromatography, 25 (5) : ( in Chinese),,, , 25 (5) : Yang Zhen2guo, Chen B in, Yang Yi2qing The phytochemical study of bupleurum yinchowerse et Y. Chinese W ild Plant Resource, (1) : 61. ( in Chinese),, , (1) : 61.
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