Chapter X. Pathogenic Escherichia coli Kyle S. Enger, MPH
|
|
- Harvey Parker
- 6 years ago
- Views:
Transcription
1 Chapter X. Pathogenic Escherichia coli Kyle S. Enger, MPH X.1 Overview Escherichia coli usually exists as a commensal bacterium in the mammalian large intestine, benefiting itself as well as the host. However, there are several well-established pathotypes of disease-causing E. coli (Kaper 2004, Nataro 1998): Enteropathogenic () o Attaches to small intestinal wall and produces attaching and effacing lesions, in which microvilli are destroyed and the bacteria become perched on pedestals on the surface of the epithelial cell. This ability is encoded on the locus of enterocyte effacement (LEE) pathogenicity island. o Causes an inflammatory response and diarrhea, but seldom in persons older than 2y; it can also be isolated from healthy older persons. o Primarily found in developing countries. Enterohemorrhagic (EHEC) o This is discussed in more detail in its own chapter. Enterotoxigenic () o Attaches to small intestinal wall. o Produces a heat-labile (LT) and/or heat-stable (ST) toxins, both of which cause secretion from the small intestinal wall, leading to mild to severe watery diarrhea. LT is an immunogenic multisubunit protein similar to cholera enterotoxin. ST is a nonimmunogenic polypeptide containing amino acids. o Primarily found in developing countries and is a major cause of diarrhea in weaned infants, as well as traveler s diarrhea. o Can be shed even by immune asymptomatic individuals. Enteroaggregative (EAEC) o Loosely classified group, some of which may be nonpathogenic. o Produces a thick biofilm ( stacked brick configuration) in the small or large intestines. o Thought to cause persistent diarrhea (lasting >14d). o Can produce many different secretory toxins and cytotoxins, but not ST or LT. Enteroinvasive (EIEC) o Actually invades the epithelial cells of the large intestine, where it multiplies. o Usually produces watery diarrhea similar to that of and, sometimes inflammatory colitis or dysentery. o Particularly closely related to Shigella sp. (which are now thought to be subgroups of E. coli); much pathogenesis from EIEC and Shigella sp. is mediated by the pwr100 virulence plasmid. Diffusely adherent (DAEC) o Attaches to the small intestinal wall and induces formation of projections which wrap around the bacterium.
2 Enterotoxigenic Escherichia coli () is the most common type of diarrheagenic E. coli (Qadri 2005). It may also be the most common cause of childhood diarrhea in the developing world, responsible for approximately 1/7 of diarrheal episodes in children aged less than 1y and almost ¼ of diarrheal episodes in 1-4 year olds (Wenneras 2004). It can also cause severe dehydrating cholera-like disease in adults (Qadri 2005). Diagnosis is complicated since many other Gram-negative bacteria produce similar toxins, so toxins as well as the E. coli bacterium must be tested for in order to yield accurate results (Wenneras 2004). can often be detected in apparently healthy people. In developing countries among healthy 0-11 month olds, and 1-4 year olds, 11.7% and 7.1%, respectively, are estimated to be colonized (Wenneras 2004). Feeding studies of or in healthy volunteers typically give 2-3g of, which neutralizes stomach acid and reduces the infectious dose (Levine et al. 1977). However, it has been suggested that food as a vehicle would have a similar acid-neutralizing effect, so feeding studies given may better represent natural foodborne infection.(levine et al. 1977) and generally have high ID 50, and partly as a consequence of this, they do not appear to be transmitted person-to-person; a study of -infected volunteers co-housed uninfected volunteers did not result in any transmission of infection.(levine 1980) Food was all served individually to the volunteers over the course of the experiment, so there was no opportunity for to spread via that route.(levine 1980) X.2 Summary of data and models There are many feeding studies of various E. coli types and strains, which can be pooled in various ways to yield different dose response models. Many of these have small sample sizes and cannot be used on their own to reliably fit a dose response model. In general, data exist spanning a wide range of doses and responses for disease. This is not the case for infection; most datasets describe high levels of infection resulting from high doses. Lower doses remain to be investigated, and dose response models for infection are therefore uncertain. Another important factor is whether the dose was given bicarbonate, which would neutralize some stomach acid and possibly increase infectivity. Haas, Rose, and Gerba (1999) fitted beta-poisson models to strains O111 (Ferguson et al. 1952) and O55 (June et al. 1953), as well as EIEC strains 4608 and 1624 (DuPont et al. 1971). Diarrhea was the response. However, it mixed data from experiments in which bacteria were given and out bicarbonate. The best available dataset using infection as a response comes from an experiment 3 dose levels, feeding EIEC to adult s (DuPont et al. 1971). Haas, Rose, and Gerba (1999) fitted a beta-poisson model to several pooled datasets describing the disease response from,, and EIEC. One strain ( O111) was found to differ from the rest, and was excluded. Powell et al. (2000) pooled 3 trial datasets (Levine et al. 1978, Beiber et al. 1998) for
3 to produce a beta-poisson model and a Weibull-gamma model. Additional pooling analyses for this chapter were conducted on the basis of pathotype ( or ), whether the dose was given bicarbonate, and the nature of the response (disease or infection), incorporating more data from the literature than the previous two published models. Since some combinations of these factors lacked data, analyses could only be done for disease (buffered or unbuffered), disease (buffered), infection (unbuffered), and infection (buffered). The pooled datasets for infection contained mostly positive responses, and therefore their behavior at low doses is very uncertain. For the pooled analyses describing disease, datasets were excluded if they contributed significantly (P < 0.05) to the -2 log likelihood of the model given the data (Haas, Rose, and Gerba 1999). Two experiments (DuPont et al. 1971) examining diarrhea from EIEC were also pooled. Table X.X: Summary of dose response data and models Experiment Reference Route Host Pathogen type type number Respons e of disease DuPont et al DuPont et al DuPont et al DuPont et al June et al Ferguson et al Coster et al Graham et al Levine et al B7A EIEC 4608 EIEC 1624 B2C O55 O111 B7A H10407 H10407 B7A milk milk milk milk Dose units cells Respon se diarrhea Best fit model None (2 doses) Optimized parameters ID 50 cells diarrhea Exponential k = 9.70E E+07 cells diarrhea Exponential k = 1.22E E+07 cells diarrhea cells diarrhea None (2 doses) Beta-Poisson (no significant trend) cells diarrhea Beta-Poisson CFU diarrhea CFU diarrhea CFU diarrhea None (2 doses) None (2 doses) cells diarrhea None (2 doses) α = 8.69E-02 N 50 = 1.94E+05 α = 2.61E-01 N 50 = 3.39E E E+06
4 Tacket et al Donnenbe rg et al Clements et al Levine et al Levine et al Levine et al B7A E2528- C1 2348/ / / TD225- C4 H10407 B7A H10407 B7A H10407 cells diarrhea cells diarrhea CFU diarrhea CFU diarrhea CFU diarrhea CFU diarrhea CFU diarrhea CFU diarrhea CFU diarrhea CFU diarrhea milk cells diarrhea or vomitin g cells diarrhea cells diarrhea None (2 doses) None (2 doses) Beta-Poisson None (2 doses) cells diarrhea α = 2.50E-01 N 50 = 9.10E E+07
5 Bieber et al Levine et al Respons e of infection DuPont et al DuPont et al DuPont et al DuPont et al Levine et al Levine et al Levine et al Levine et al Tacket et al B /69 E851/17 1 E74/68 B7A EIEC 4608 EIEC 1624 B2C B7A B7A B7A E2528- C1 2348/69 cells diarrhea CFU diarrhea Exponential k = 1.97E E+08 CFU diarrhea CFU diarrhea CFU diarrhea milk milk milk milk cells cells cells cells cells cells cells cells CFU positive isolation positive isolation positive isolation positive isolation positive isolation positive isolation positive isolation positive isolation sheddin g in None (2 doses) None (poor fit) None (all responses negative) None (2 doses) No dose response trend Beta-Poisson None (2 doses) None (2 doses) α = 1.55E-01 N 50 = 2.11E E+006
6 Donnenbe rg et al Donnenbe rg et al Donnenbe rg et al Levine et al Levine et al Levine et al Levine et al / / /69 E851/71 E74/68 milk CFU CFU CFU cells CFU CFU CFU sheddin g in sheddin g in sheddin g in positive isolation positive isolation positive isolation positive isolation None (2 doses) All but 1 subject positive None (2 doses) All subjects positive All but 1 subject positive Pooled models 38, 39, 40, 42, 99, , 216, , 143, 144, 145, 147, 151, 161, 162, 163, 164, 168, 169, 170, , 42, 99, , 100, 166 See above; fit by Haas, Rose, and Gerba 1999 See above; fit by Powell 2000,, EIEC See above milk or See above (no ) See above (no CFU diarrhea Beta-Poisson CFU diarrhea Beta-Poisson CFU diarrhea Beta-Poisson CFU diarrhea Beta-Poisson CFU infectio n Beta-Poisson α = 1.78E-01 N 50 = 8.60E+07 α = 2.21E-01 N 50 = 6.85E+07 α = 7.54E-02 N 50 = 1.70E+06 α = 2.06E-01 N 50 = 1.28E+08 α = 3.75E-01 N 50 = 1.78E E E E E E+005
7 153, 157, 159, 214, 216, , 156, 158, 160, 219, 220, , 40 See above See above DuPont et al EIEC ) CFU diarrhea Beta-Poisson Exponential infectio CFU (no low dose n data) milk α = 1.62E-01 N 50 = 9.98E+07 k = 1.95E E E+005 cells diarrhea Exponential k = 1.07E E+007
8 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for experiment 39. Table 2. Model data for Escherichia coli (EIEC 4608) in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 4 ) (10 6 ) (10 8 ) DuPont et al. (1971) Exponential Beta-Poisson Exponential fits better than beta-poisson; cannot reject good fit for exponential. Table 4. Optimized parameter(k) and ID 50 for best fitting model (exponential); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% k 9.70E E E E E E E-08 ID E E E E E E E+08 Figure 1. Histogram of k parameter for exponential model. Figure 2. Exponential model plot, confidence bounds around the optimized model.
9 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for experiment 40. Table 2. Model data for Escherichia coli (EIEC 1624) in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 4 ) (10 6 ) (10 8 ) DuPont et al. (1971) Exponential Beta-Poisson Exponential fits better than beta-poisson; cannot reject good fit for exponential. Table 4. Optimized parameter(k) and ID 50 for best fitting model (exponential); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% k 1.22E E E E E E E-07 ID E E E E E E E+08 Figure 1. Histogram of k parameter for exponential model. Figure 2. Exponential model plot, confidence bounds around the optimized model.
10 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for experiment 42. Table 2. Model data for Escherichia coli ( O55 (in paper as type 55, B5 )) in the Dose Positive Negative Total 1.4 (10 8 ) (10 9 ) (10 9 ) (10 10 ) June et al. (1953) Model Table 3. Best fitting model determination Devianc e Exponential 53.3 Δ 48.7 Degrees of freedom Beta-Poisson χ ,1 χ ,m-k Beta-Poisson fits better than exponential; cannot reject good fit for beta-poisson. Table 4. Optimized parameters and LD 50 for best fitting model (beta-poisson); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% α 7.23E E E E E E E-01 N E E E E E E E+008 Figure 1. Parameter scatter plot for beta-poisson model. Ellipses signify the 0.90, 0.95 and 0.99 confidence intervals. Figure 2. Beta-Poisson model plot, confidence bounds around the optimized model.
11 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for experiment 43. Table 2. Model data for Escherichia coli ( O111 (in paper as E. coli 111, B4 )) in the Dose Positive Negative Total 7 (10 6 ) (10 8 ) (10 9 ) (10 9 ) Ferguson et al. (1952) Model Table 3. Best fitting model determination Devianc e Exponential 51.4 Δ 45.0 Degrees of freedom Beta-Poisson χ ,1 χ ,m-k Neither the exponential nor beta-poisson fits well; beta-poisson is less bad. Table 4. Optimized parameters and LD 50 for best fitting model (beta-poisson); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% α 2.61E E E E E E E+00 N E E E E E E E+007 Figure 1. Parameter scatter plot for beta-poisson model. Ellipses signify the 0.90, 0.95 and 0.99 confidence intervals. Figure 2. Beta-Poisson model plot, confidence bounds around the optimized model.
12 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for experiment 165. Table 2. Model data for Escherichia coli ( (ST)) in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 6 ) (10 8 ) (10 10 ) Levine et al. (1977) Exponential Beta-Poisson Beta-Poisson fits better than exponential; cannot reject good fit for beta-poisson. Table 4. Optimized parameters and LD 50 for best fitting model (beta-poisson); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% α 2.50E E E E E E E+00 N E E E E E E E+008 Figure 1. Parameter scatter plot for beta-poisson model. Ellipses signify the 0.90, 0.95 and 0.99 confidence intervals. Figure 2. Beta-Poisson model plot, confidence bounds around the optimized model.
13 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for experiment 214. Table 2. Model data for Escherichia coli ( B171-8 (serotype O11:NM)) in the Dose Positive Negative Total 5 (10 8 ) (10 9 ) (10 10 ) Bieber et al. (1998) Model Table 3. Best fitting model determination Devianc e Exponential 0.6 Δ NA Degrees of freedom Beta-Poisson NA 1 2 χ , χ ,m-k NA Exponential fits better than beta-poisson; cannot reject good fit for exponential. Table 4. Optimized parameter(k) and ID 50 for best fitting model (exponential); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% k 1.97E E E E E E E-08 ID E E E E E E E+08 Figure 1. Histogram of k parameter for exponential model. Figure 2. Exponential model plot, confidence bounds around the optimized model.
14 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for experiment 98. Table 2. Model data for Escherichia coli (EIEC 1624) in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 4 ) (10 6 ) (10 8 ) DuPont et al. (1971) Exponential Beta-Poisson Beta-Poisson fits better than exponential; cannot reject good fit for beta-poisson. Table 4. Optimized parameters and ID 50 for best fitting model (beta-poisson); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% α 1.55E E E E E E E+02 N E E E E E E E+140 Figure 1. Parameter scatter plot for beta-poisson model. Ellipses signify the 0.90, 0.95 and 0.99 confidence intervals. Figure 2. Beta-Poisson model plot, confidence bounds around the optimized model.
15 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output, pooled experiments 38, 39, 40, 42, 99, 144. Table 2. Model data for E. coli disease (,, EIEC) in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 4 ) (10 4 ) (10 6 ) (10 6 ) (10 8 ) Exponential Beta-Poisson Beta-Poisson fits better than exponential; cannot reject good fit for beta-poisson. 1 (10 8 ) (10 8 ) (10 8 ) (10 8 ) (10 8 ) (10 9 ) (10 9 ) (10 10 ) (10 10 ) (10 10 ) DuPont et al. (1971), June et al. (1953), Graham et al. (1983) Table 4. Optimized parameters for best fitting model (beta-poisson); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% α 1.78E E E E E E E-01 N E E E E E E E+008
16 Figure 1. Parameter scatter plot for beta-poisson model. Ellipses signify the 0.90, 0.95 and 0.99 confidence intervals. Figure 2. Beta-Poisson model plot, confidence bounds around the optimized model.
17 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for pooled experiments 214 and 216 and 217. Table 2. Model data for disease in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 6 ) (10 6 ) (10 8 ) (10 8 ) (10 9 ) Exponential Beta-Poisson Beta-Poisson fits better than exponential; cannot reject good fit for beta-poisson. 2 (10 10 ) (10 10 ) Levine et al. (1978), Bieber et al. (1998) Table 4. Optimized parameters for best fitting model (beta-poisson); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% α 2.21E E E E E E E+03 N E E E E E E E+009
18 Figure 1. Parameter scatter plot for beta-poisson model. Ellipses signify the 0.90, 0.95 and 0.99 confidence intervals. Figure 2. Beta-Poisson model plot, confidence bounds around the optimized model.
19 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for pooled experiments 142, 143, 144, 145, 147, 151, 161, 162, 163, 164, 168, 169, 170, 172. Table 2. Model data for disease, buffered, in the Dose Positive Negative Total Model Table 3. Best fitting model determination Deviance Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 6 ) (10 7 ) (10 8 ) (10 8 ) (10 8 ) Exponential Beta-Poisson Beta-Poisson fits better than exponential; cannot reject good fit for beta-poisson. 1 (10 8 ) (10 8 ) (10 8 ) (10 8 ) (10 8 ) (10 8 ) (10 8 ) (10 9 ) (10 9 ) (10 9 ) (10 10 ) (10 10 ) (10 10 ) (10 10 ) Coster et al. (2007), Graham et al. (1983), Levine et al. (1979), Levine et al. (1980), Clements et al. (1981), Levine et al. (1982) Table 4. Optimized parameters & ID 50 for best fitting model (beta-poisson); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% α 7.54E E E E E E E-01 N E E E E E E E+007
20 Figure 1. Parameter scatter plot for beta-poisson model. Ellipses signify the 0.90, 0.95 and 0.99 confidence intervals. Figure 2. Beta-Poisson model plot, confidence bounds around the optimized model.
21 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for pooled experiments 38, 42, 99, 165. Table 2. Model data for disease, unbuffered, in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 6 ) (10 8 ) (10 8 ) (10 8 ) (10 8 ) Exponential Beta-Poisson Beta-Poisson fits better than exponential; cannot reject good fit for beta-poisson. 1.7 (10 9 ) (10 9 ) (10 10 ) (10 10 ) (10 10 ) (10 10 ) DuPont et al. (1971), June et al. (1953), Levine et al. (1977) Table 4. Optimized parameters and ID 50 for best fitting model (beta-poisson); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% α 2.06E E E E E E E-01 N E E E E+07 2E E E+008
22 Figure 1. Parameter scatter plot for beta-poisson model. Ellipses signify the 0.90, 0.95 and 0.99 confidence intervals. Figure 2. Beta-Poisson model plot, confidence bounds around the optimized model.
23 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for pooled experiments 96, 100, 166. Table 2. Model data for infection, unbuffered, in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 6 ) (10 8 ) (10 8 ) (10 8 ) (10 10 ) Exponential Beta-Poisson Beta-Poisson fits better than exponential; cannot reject good fit for beta-poisson. 1 (10 10 ) (10 10 ) DuPont et al. (1971), Levine et al. (1977) Table 4. Optimized parameters and ID 50 for best fitting model (beta-poisson); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% α 3.75E E E E E E E+01 N E E E E E E E+006
24 Figure 1. Parameter scatter plot for beta-poisson model. Ellipses signify the 0.90, 0.95 and 0.99 confidence intervals. Figure 2. Beta-Poisson model plot, confidence bounds around the optimized model.
25 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for pooled experiments 153, 157, 159, 214, 216, 217. Table 2. Model data for disease, buffered, in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 6 ) (10 6 ) (10 8 ) (10 8 ) (10 9 ) Exponential Beta-Poisson Beta-Poisson fits better than exponential; cannot reject good fit for beta-poisson. 1 (10 10 ) (10 10 ) (10 10 ) (10 10 ) (10 10 ) (10 10 ) Tacket et al. (2000), Donnenberg et al. (1998), Bieber et al. (1998), Levine et al. (1978) Table 4. Optimized parameters and ID 50 for best fitting model (beta-poisson); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% α 1.62E E E E E E E-01 N E E E E E E E+009
26 Figure 1. Parameter scatter plot for beta-poisson model. Ellipses signify the 0.90, 0.95 and 0.99 confidence intervals. Figure 2. Beta-Poisson model plot, confidence bounds around the optimized model.
27 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for pooled experiments 154, 156, 158, 160, 219, 220, 221. Table 2. Model data for, infection, buffered, in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 6 ) (10 6 ) (10 6 ) (10 8 ) (10 8 ) Exponential Beta-Poisson Exponential fits better than beta-poisson; cannot reject good fit for exponential. 9 (10 8 ) (10 10 ) (10 10 ) (10 10 ) (10 10 ) (10 10 ) (10 10 ) (10 10 ) Tacket et al. (2000), Donnenberg et al. (1993), Levine et al. (1978) Table 4. Optimized parameter(k) and ID 50 for best fitting model (exponential); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% k 1.95E E E E E E E-06 ID E E E E E E E+05
28 Figure 1. Histogram of k parameter for exponential model. Figure 2. Exponential model plot, confidence bounds around the optimized model.
29 X.3. Optimized models and fitting analysis X.3.1. Data and optimization output for pooled experiments 39 and 40. Table 2. Model data for EIEC disease, unbuffered, in the Dose Positive Negative Total Model Table 3. Best fitting model determination Devianc e Δ Degrees of freedom χ ,1 χ ,m-k 1 (10 4 ) (10 4 ) (10 6 ) (10 6 ) (10 8 ) Exponential Beta-Poisson Exponential fits better than beta-poisson; cannot reject good fit for exponential. 1 (10 8 ) DuPont et al. (1971) Table 4. Optimized parameter(k) and ID 50 for best fitting model (exponential); percentiles from 10 4 bootstrap iterations Parameter MLE estimate Percentiles 0.5% 2.5% 5% 95% 97.5% 99.5% k 1.07E E E E E E E-08 ID E E E E E E E+08 Figure 1. Histogram of k parameter for exponential model. Figure 2. Exponential model plot, confidence bounds around the optimized model.
30 References Bieber, D. et al., Type IV pili, transient bacterial aggregates, and virulence of enteropathogenic Escherichia coli. Science (New York, N.Y.), 280(5372), pp Clements, M.L. et al., Lactobacillus prophylaxis for diarrhea due to enterotoxigenic Escherichia coli. Antimicrobial Agents and Chemotherapy, 20(1), pp Coster, T.S. et al., Immune response, ciprofloxacin activity, and gender differences after experimental challenge by two strains of enterotoxigenic Escherichia coli. Infection and Immunity, 75(1), pp Donnenberg, M.S. et al., Role of the eaea gene in experimental enteropathogenic Escherichia coli infection. The Journal of Clinical Investigation, 92(3), pp Donnenberg, M.S. et al., Effect of prior experimental enteropathogenic Escherichia coli infection on illness following homologous and heterologous rechallenge. Infection and Immunity, 66(1), pp DuPont, H.L. et al., Pathogenesis of Escherichia coli diarrhea. The New England Journal of Medicine, 285(1), pp.1-9. Ferguson, W.W. & June, R.C., Experiments on feeding adult volunteers Escherichia coli 111, B4, a coliform organism associated infant diarrhea. American Journal of Hygiene, 55(2), pp Graham, D.Y., Estes, M.K. & Gentry, L.O., Double-blind comparison of bismuth subsalicylate and placebo in the prevention and treatment of enterotoxigenic Escherichia coli-induced diarrhea in volunteers. Gastroenterology, 85(5), pp Haas, C.N., Rose, J.B. & Gerba, C.P., Quantitative Microbial Risk Assessment, John Wiley & Sons, Inc. June, R.C., Ferguson, W.W. & Worfel, M.T., Experiments in feeding adult volunteers Escherichia coli 55, B5, a coliform organism associated infant diarrhea. American Journal of Hygiene, 57(2), pp Kaper, J.B., Nataro, J.P. & Mobley, H.L., Pathogenic Escherichia coli. Nature Reviews. Microbiology, 2(2), pp Levine, M.M. et al., Escherichia coli strains that cause diarrhoea but do not produce heatlabile or heat-stable enterotoxins and are non-invasive. Lancet, 1(8074), pp Levine, M.M. et al., Reactogenicity, immunogenicity and efficacy studies of Escherichia coli type-1 somatic pili parenteral vaccine in man. Scandinavian Journal of Infectious Diseases, Suppl. 33, pp Levine, M.M. et al., Diarrhea caused by Escherichia coli that produce only heat-stable enterotoxin. Infection and Immunity, 17(1), pp Levine, M.M. et al., Immunity to enterotoxigenic Escherichia coli. Infection and Immunity, 23(3), pp Levine, M.M. et al., Lack of person-to-person transmission of enterotoxigenic Escherichia coli despite close contact. American Journal of Epidemiology, 111(3), pp
31 Nataro, J.P. & Kaper, J.B., Diarrheagenic Escherichia coli. Clinical Microbiology Reviews, 11(1), pp Powell, M.R., Dose-response envelope for Escherichia coli O157:H7. Quantitative Microbiology, 2, pp Qadri, F. et al., Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clinical Microbiology Reviews, 18(3), pp Tacket, C.O. et al., Role of EspB in experimental enteropathogenic Escherichia coli infection. Infection and Immunity, 68(6), pp Wennerås, C. & Erling, V., Prevalence of enterotoxigenic Escherichia coli-associated diarrhoea and carrier state in the developing world. Journal of Health, Population, and Nutrition, 22(4), pp
Risk Assessment of Staphylococcus aureus and Clostridium perfringens in ready to eat Egg Products
Risk Assessment of Staphylococcus aureus and Clostridium perfringens in ready to eat Egg Products Introduction Egg products refer to products made by adding other types of food or food additives to eggs
More informationCONTROL OF SUBSTRATE UTILIZATION BY O-ISLANDS AND S- LOOPS IN ESCHERICHIA COLI O157:H7
CONTROL OF SUBSTRATE UTILIZATION BY O-ISLANDS AND S- LOOPS IN ESCHERICHIA COLI O157:H7 SARAH-JO PAQUETTE B. Sc. Biochemistry, University of Lethbridge, 2005 A Thesis Submitted to the School of Graduate
More informationFOR RUMINANTS. kemin.com/guthealth
FOR RUMINANTS kemin.com/guthealth What is CLOSTAT? CLOSTAT contains a proprietary, patented strain of Bacillus subtilis PB6. PB6 is a unique, naturally occurring, spore-forming microorganism. Kemin has
More informationIn vitro the effect of intestinal normal flora on some pathogenic bacteria.
In vitro the effect of intestinal normal flora on some pathogenic bacteria. Abstract: Dr.abbass shaker Ali adel Leena abd Al-Redha The effect of two types of intestinal bacterial normal floral ( and klebsiella)
More informationGram negative bacilli
Gram negative bacilli 1-Enterobacteriaceae Gram negative bacilli-rods Enterobacteriaceae Are everywhere Part of normal flora of humans and most animals They are cause of -30-35% septisemia -more than 70%
More informationBacteria Outline. 1. Overview. 2. Structural & Functional Features. 3. Taxonomy. 4. Communities
Bacteria Outline 1. Overview 2. Structural & Functional Features 3. Taxonomy 4. Communities Bacteria - Taxonomy PHYLUM CLASS ORDER FAMILY GENUS SPECIES SUB-SPECIES & STRAINS Bacteria - Phyla Firmicutes
More informationIndicator Organisms SCI5508
Indicator Organisms SCI5508 Indicator Organisms REFLECTS microbiological quality organisms and/or their metabolic products whose presence in given foods at certain levels may be used to assess existing
More informationCairo University Faculty of Veterinary Medicine Department of Microbiology. Thesis Presented By
Cairo University Faculty of Veterinary Medicine Department of Microbiology STUDIES ON ESCHERICHIA COLI IN CALVES Thesis Presented By Rehab Fathy El-Shafey El-Sayed (B.V.SC., Cairo University, 2000) For
More informationYear 09 Science Learning Cycle 5 Overview
e Year 09 Science Learning Cycle 5 Overview Learning Cycle Overview: Biology How do we keep your body healthy L01 4.3.1.1 Communicable (infectious) disease L02 4.3.1.2 Viral diseases L03 4.3.1.3 Bacterial
More informationTHE IDENTIFICATION OF TWO UNKNOWN BACTERIA AFUA WILLIAMS BIO 3302 TEST TUBE 3 PROF. N. HAQUE 5/14/18
THE IDENTIFICATION OF TWO UNKNOWN BACTERIA AFUA WILLIAMS BIO 3302 TEST TUBE 3 PROF. N. HAQUE Introduction: The identification of bacteria is important in order for us to differentiate one microorganism
More informationThe Evolution of Infectious Disease
The Evolution of Infectious Disease Why are some bacteria pathogenic to humans while other (closely-related) bacteria are not? This question can be approached from two directions: 1.From the point of view
More informationNIH Public Access Author Manuscript Ann N Y Acad Sci. Author manuscript; available in PMC 2012 April 13.
NIH Public Access Author Manuscript Published in final edited form as: Ann N Y Acad Sci. 2009 May ; 1165: 169 174. doi:10.1111/j.1749-6632.2009.04060.x. Tight Junctions and Enteropathogenic E. coli Andrew
More informationTwo-sample inference: Continuous Data
Two-sample inference: Continuous Data November 5 Diarrhea Diarrhea is a major health problem for babies, especially in underdeveloped countries Diarrhea leads to dehydration, which results in millions
More informationAn introduction to the Serotypes, Pathotypes and Phylotypes of Escherichia coli
International Journal of Microbiology and Allied Sciences (IJOMAS) ISSN: 2382-5537 August 2015, 2(1):9-16 IJOMAS, 2015 Review Article Page: 9-16 An introduction to the Serotypes, Pathotypes and Phylotypes
More informationThe Bacterial Causes of Camel-calf (Camelus dromedarius) Diarrhea in Eastern Sudan.
Proceedings of the Third Annual Meeting for Animal Production Under Arid Conditions, Vol. 2: 132-137 1998United Arab Emirates University. The Bacterial Causes of Camel-calf (Camelus dromedarius) Diarrhea
More informationThe Pangenome Structure of Escherichia coli: Comparative Genomic Analysis of E. coli Commensal and Pathogenic Isolates
JOURNAL OF BACTERIOLOGY, Oct. 2008, p. 6881 6893 Vol. 190, No. 20 0021-9193/08/$08.00 0 doi:10.1128/jb.00619-08 Copyright 2008, American Society for Microbiology. All Rights Reserved. The Pangenome Structure
More information1 Disease Spread Model
Technical Appendix for The Impact of Mass Gatherings and Holiday Traveling on the Course of an Influenza Pandemic: A Computational Model Pengyi Shi, Pinar Keskinocak, Julie L Swann, Bruce Y Lee December
More informationPHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF Escherichia. coli ISOLATES RECOVERED FROM TREATED WASTEWATER
PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF Escherichia coli ISOLATES RECOVERED FROM TREATED WASTEWATER EFFLUENT AND RECEIVING AQUATIC MILIEU IN DURBAN, SOUTH AFRICA BY LEANNE PILLAY Submitted in fulfilment
More informationIntroduction to Microbiology. CLS 212: Medical Microbiology Miss Zeina Alkudmani
Introduction to Microbiology CLS 212: Medical Microbiology Miss Zeina Alkudmani Microbiology Micro- means very small (that needs a microscope to see). Microbiology is the study of very small living organisms.
More informationThe Scope of Food Microbiology p. 1 Micro-organisms and Food p. 2 Food Spoilage/Preservation p. 2 Food Safety p. 4 Fermentation p.
The Scope of Food Microbiology p. 1 Micro-organisms and Food p. 2 Food Spoilage/Preservation p. 2 Food Safety p. 4 Fermentation p. 4 Microbiological Quality Assurance p. 4 Micro-organisms and Food Materials
More informationContents. The Trajectory of Food Microbiology 3. Spores and heir Significance 39. Factors That Influence Microbes infoods 11
Preface xv About the Authors xvii SECTION 1 Basics of Food Microbiology The Trajectory of Food Microbiology 3 Introduction 3 Who's on First? 3 Food Microbiology, Past and Present 4 To the Future and Beyond
More informationTyphoid Fever Dr. KHALID ALJARALLAH
Dr. KHALID ALJARALLAH kaljarallah@kfmc.med.sa Main objectives General characteristics (G-, Rod, Facultative anaerobe..etc,) Natural Habitat and transmission root Symptoms Pathogenicity Diagnosis and treatment
More informationINVESTIGATING BACTERIAL LIPOPOLYSACCHARIDES AND INTERACTIONS WITH ANTIMICROBIAL PEPTIDES
INVESTIGATING BACTERIAL LIPOPOLYSACCHARIDES AND INTERACTIONS WITH ANTIMICROBIAL PEPTIDES by Joshua Strauss A Thesis Submitted to the Faculty of the WORCESTER POLYTECHNIC INSTITUTE in partial fulfillment
More informationBACTERIAL PHYSIOLOGY SMALL GROUP. Monday, August 25, :00pm. Faculty: Adam Driks, Ph.D. Alan Wolfe, Ph.D.
BACTERIAL PHYSIOLOGY SMALL GROUP Monday, August 25, 2014 1:00pm Faculty: Adam Driks, Ph.D. Alan Wolfe, Ph.D. Learning Goal To understand how bacterial physiology applies to the diagnosis and treatment
More informationSeminar 2 : Good Bugs
Seminar 2 : Good Bugs Part 2 Viruses What is a virus? Microscopic particles that infect other organisms and can only replicate within a host cell Contain either contain DNA or RNA surrounded by a protective
More informationThursday. Threshold and Sensitivity Analysis
Thursday Threshold and Sensitivity Analysis SIR Model without Demography ds dt di dt dr dt = βsi (2.1) = βsi γi (2.2) = γi (2.3) With initial conditions S(0) > 0, I(0) > 0, and R(0) = 0. This model can
More informationAnalysis of Longitudinal Data. Patrick J. Heagerty PhD Department of Biostatistics University of Washington
Analysis of Longitudinal Data Patrick J Heagerty PhD Department of Biostatistics University of Washington Auckland 8 Session One Outline Examples of longitudinal data Scientific motivation Opportunities
More informationMolecular Evolution of Large Virulence Plasmid in Shigella Clones and Enteroinvasive Escherichia coli
INFECTION AND IMMUNITY, Oct. 2001, p. 6303 6309 Vol. 69, No. 10 0019-9567/01/$04.00 0 DOI: 10.1128/IAI.69.10.6303 6309.2001 Copyright 2001, American Society for Microbiology. All Rights Reserved. Molecular
More informationA mathematical and computational model of necrotizing enterocolitis
A mathematical and computational model of necrotizing enterocolitis Ivan Yotov Department of Mathematics, University of Pittsburgh McGowan Institute Scientific Retreat March 10-12, 2008 Acknowledgment:
More informationCOMMISSION REGULATION (EU)
26.5.2011 Official Journal of the European Union L 138/45 COMMISSION REGULATION (EU) No 517/2011 of 25 May 2011 implementing Regulation (EC) No 2160/2003 of the European Parliament and of the Council as
More informationThis is a repository copy of Evidence for antibiotic induced Clostridium perfringens diarrhoea.
This is a repository copy of Evidence for antibiotic induced Clostridium perfringens diarrhoea. White Rose Research Online URL for this paper: http://eprints.whiterose.ac.uk/256/ Article: Modi, N. and
More informationPDF // IS BACTERIA A PROKARYOTE OR EUKARYOTE
19 January, 2018 PDF // IS BACTERIA A PROKARYOTE OR EUKARYOTE Document Filetype: PDF 222.61 KB 0 PDF // IS BACTERIA A PROKARYOTE OR EUKARYOTE How to Tell the Difference Between Prokaryotes and Eukaryotes.
More informationSalmonella enteritidis Identification and Isolation
Department of Microbiology, Qom Branch, Islamic Azad University. Qom, Iran Start Here Advisor Dr.Mohsen Zargar Consulting Advisor Dr.Taghi Salehi Zahraei Presented by Zeinab Yazdanpanah 1 Outcome Enterobacteriaceae
More informationNatural Genetic Resistance to Infection
Natural Genetic Resistance to Infection The Discovery of Natural Determinants of Susceptibility to Infection in Cattle, especially Tarentaise Steve A Carlson, DVM PhD Tim A Day, PhD PSR Genetics, LLC Scott
More informationEpithelial cell signaling responses to enterohemorrhagic Escherichia coli infection
Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 100(Suppl. I): 199-203, 2005 199 Epithelial cell signaling responses to enterohemorrhagic Escherichia coli infection Peter JM Ceponis ++, Jason D Riff +++, Philip
More informationHorizontal transfer and pathogenicity
Horizontal transfer and pathogenicity Victoria Moiseeva Genomics, Master on Advanced Genetics UAB, Barcelona, 2014 INDEX Horizontal Transfer Horizontal gene transfer mechanisms Detection methods of HGT
More informationBy Eliza Bielak Bacterial Genomics and Epidemiology, DTU-Food Supervised by Henrik Hasman, PhD
By Eliza Bielak Bacterial Genomics and Epidemiology, DTU-Food elibi@food.dtu.dk Supervised by Henrik Hasman, PhD 1. Introduction to plasmid biology 2. Plasmid encoded resistance to β- lactams (basic theories)
More informationCRISPR-SeroSeq: A Developing Technique for Salmonella Subtyping
Department of Biological Sciences Seminar Blog Seminar Date: 3/23/18 Speaker: Dr. Nikki Shariat, Gettysburg College Title: Probing Salmonella population diversity using CRISPRs CRISPR-SeroSeq: A Developing
More informationKEY CONCEPTS AND PROCESS SKILLS. 2. Most infectious diseases are caused by microbes.
Who s Who? 44 40- to 1 50-minute session ACTIVITY OVERVIEW I N V E S T I O N I G AT SUMMARY Cards with images of the major groups of disease-causing microbes (s, bacteria, and es) are presented. Students
More informationMicrobiota: Its Evolution and Essence. Hsin-Jung Joyce Wu "Microbiota and man: the story about us
Microbiota: Its Evolution and Essence Overview q Define microbiota q Learn the tool q Ecological and evolutionary forces in shaping gut microbiota q Gut microbiota versus free-living microbe communities
More informationIntroductory Microbiology Dr. Hala Al Daghistani
Introductory Microbiology Dr. Hala Al Daghistani Why Study Microbes? Microbiology is the branch of biological sciences concerned with the study of the microbes. 1. Microbes and Man in Sickness and Health
More informationVIRULENCE. Vibrio cholerae Yersinia Shigella
VIRULENCE How do all the sensing systems we ve looked at so far come together to control the response of a pathogen to its host and what is the response of the host 3 examples Vibrio cholerae Yersinia
More informationno.1 Raya Ayman Anas Abu-Humaidan
no.1 Raya Ayman Anas Abu-Humaidan Introduction to microbiology Let's start! As you might have concluded, microbiology is the study of all organisms that are too small to be seen with the naked eye, Ex:
More informationRole of GIS in Tracking and Controlling Spread of Disease
Role of GIS in Tracking and Controlling Spread of Disease For Dr. Baqer Al-Ramadan By Syed Imran Quadri CRP 514: Introduction to GIS Introduction Problem Statement Objectives Methodology of Study Literature
More informationImmunization of mice with live oral vaccine based on a Salmonella enterica (sv Typhimurium) aroa strain expressing the Escherichia coli O111 O antigen
Article available online at http://www.idealibrary.com on Microbial Pathogenesis 1999; 27: 55 59 Article No. mpat.1999.286 MICROBIAL PATHOGENESIS SHORT COMMUNICATION Immunization of mice with live oral
More informationAsymptotic Confidence Ellipses of Parameters for the Beta-Poisson Dose-Response Model
Thailand Statistician January 2012; 10(1) : 15-39 http://statassoc.or.th Contributed paper Asymptotic Confidence Ellipses of Parameters for the Noppadol Angkanavisal [a,b], Kamon Budsaba* [a,b] and Andrei
More informationInteractions between symbiotic microbes, their mammalian host, and invading pathogens
Interactions between symbiotic microbes, their mammalian host, and invading pathogens Vanessa Sperandio UT Southwestern Medical Center Depts. Microbiology and Biochemistry To have a quorum you need chemicals
More informationA broad spectrum vaccine to prevent invasive Salmonella Infections for sub- Saharan Africa
A broad spectrum vaccine to prevent invasive Salmonella Infections for sub- Saharan Africa Myron M Levine, Raphael Simon, Sharon Tennant, James Galen, Andrew Lees, Velupillai Puvanesarajah, Ellen Higginson,
More informationChapter 11 Survival strategies of pathogens in the host. a.a
Chapter 11 Survival strategies of pathogens in the host a.a. 2017-18 Survival strategies of pathogens In order to survive in a host a pathogen must be able to Penetrate into the body Attach to host cells
More informationVPM 201: Veterinary Bacteriology and Mycology 6-7/10/2010. LABORATORY 5a - ENTEROBACTERIACEAE
VPM 201: Veterinary Bacteriology and Mycology 6-7/10/2010 LABORATORY 5a - ENTEROBACTERIACEAE A large family of gram-negative bacilli. They grow readily on common culture media. Organisms are separated
More informationBayesian inference and model selection for stochastic epidemics and other coupled hidden Markov models
Bayesian inference and model selection for stochastic epidemics and other coupled hidden Markov models (with special attention to epidemics of Escherichia coli O157:H7 in cattle) Simon Spencer 3rd May
More informationVocabulary- Bacteria (34 words)
Biology II BACTERIA Vocabulary- Bacteria (34 words) 1. Prokaryote 21. phototroph 2. Peptidoglycan 22. chemotroph 3. Methanogen 23. obligate anaerobe 4. Halophile 24. facultative anaerobe 5. Thermoacidophile
More informationIN VIETNAM. Benjamin Robert Sobkowiak. A thesis submitted for the degree of Doctorate of Philosophy. Department of Genetics, Evolution and Environment
THE GENETICS AND EPIDEMIOLOGY OF SHIGELLA SONNEI AND SHIGELLA FLEXNERI IN VIETNAM Benjamin Robert Sobkowiak A thesis submitted for the degree of Doctorate of Philosophy Department of Genetics, Evolution
More informationExplain your answer:
Biology Midterm Exam Review Introduction to Biology and the Scientific Method Name: Date: Hour: 1. Biology is the study of: 2. A living thing is called a(n): 3. All organisms are composed of: 4. The smallest
More informationHypothesis testing for µ:
University of California, Los Angeles Department of Statistics Statistics 10 Elements of a hypothesis test: Hypothesis testing Instructor: Nicolas Christou 1. Null hypothesis, H 0 (always =). 2. Alternative
More informationDisplacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli
Journal of Medical Microbiology (2003), 52, 925 930 DOI 10.1099/jmm.0.05009-0 Displacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli Yuan-Kun Lee, 1 Kim-Yoong Puong, 1 Arthur
More informationQuantitative Risk Assessment of Salmonella spp. in Fermented Pork Sausage (Nham)
Kasetsart J. (Nat. Sci.) 38 : 52-65 (24) Quantitative Risk Assessment of Salmonella spp. in Fermented Pork Sausage (Nham) Sukhuntha Osiriphun, Adisak Pongpoolponsak 2 and Kooranee Tuitemwong 3 ABSTRACT
More informationGenetic Variation: The genetic substrate for natural selection. Horizontal Gene Transfer. General Principles 10/2/17.
Genetic Variation: The genetic substrate for natural selection What about organisms that do not have sexual reproduction? Horizontal Gene Transfer Dr. Carol E. Lee, University of Wisconsin In prokaryotes:
More informationDepartment of Mathematics. Mathematical study of competition between Staphylococcus strains within the host and at the host population level
Department of Mathematics Mathematical study of competition between Staphylococcus strains within the host and at the host population level MATH554: Main Dissertation Written by Nouf Saleh Alghamdi ID
More informationBrief history of life on Earth
Brief history of life on Earth 4.6 Billion Years ago: Earth forms 3.6 Billion Years ago : First life on the planet (Prokaryotes = Bacteria) 2.8 Billion Years ago : First eukaryotic life (also microbial
More informationComparing two independent samples
In many applications it is necessary to compare two competing methods (for example, to compare treatment effects of a standard drug and an experimental drug). To compare two methods from statistical point
More informationModeling the Spread of Epidemic Cholera: an Age-Structured Model
Modeling the Spread of Epidemic Cholera: an Age-Structured Model Alen Agheksanterian Matthias K. Gobbert November 20, 2007 Abstract Occasional outbreaks of cholera epidemics across the world demonstrate
More informationThe outer membrane of Borrelia 7/3/2014. LDA Conference Richard Bingham 1. The Outer Membrane of Borrelia; The Interface Between Them and Us
The Outer Membrane of Borrelia; The Interface Between Them and Us Richard Bingham The University of Huddersfield Lecture Outline I will give an overview of the outer membrane of Borrelia I will present
More informationStudies on Pathogenesis and Immunity to Turkey Clostridial Dermatitis. K.V. Nagaraja and Anil Thachil
Studies on Pathogenesis and Immunity to Turkey Clostridial Dermatitis K.V. Nagaraja and Anil Thachil Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Ave, St.
More informationIntroduction to microbiology
Sulaimani University College of Pharmacy Microbiology Introduction to microbiology Dr. Abdullah Ahmed Hama PhD. Molecular Medical Parasitology abdullah.hama@spu.edu.iq 1 Definition Microbiology: is the
More informationLESSON 1.3 WORKBOOK. Bacterial structures. Workbook Lesson 1.3
Colonize the ability of bacteria to adapt to permanently inhabit our bodies. Capsule an external layer made of sugars that surrounds some bacteria. Cell wall an external layer surrounding the plasma membrane
More informationFernando Leite, Connie Gebhart, Randall Singer, Richard Isaacson. University of Minnesota, St. Paul, MN
VACCINATION AGAINST LAWSONIA INTRACELLULARIS DECREASES SHEDDING OF SALMONELLA ENTERICA SEROVAR TYPHIMURIUM IN CO-INFECTED PIGS AND CHANGES THE HOST GUT MICROBIOME Fernando Leite, Connie Gebhart, Randall
More informationProkaryotes & Viruses. Multiple Choice Review. Slide 1 / 47. Slide 2 / 47. Slide 3 / 47
New Jersey enter for Teaching and Learning Slide 1 / 47 Progressive Science Initiative This material is made freely available at www.njctl.org and is intended for the non-commercial use of students and
More informationSupplementary materials Quantitative assessment of ribosome drop-off in E. coli
Supplementary materials Quantitative assessment of ribosome drop-off in E. coli Celine Sin, Davide Chiarugi, Angelo Valleriani 1 Downstream Analysis Supplementary Figure 1: Illustration of the core steps
More informationHACCP: INTRODUCTION AND HAZARD ANALYSIS
Food Hygiene HACCP: INTRODUCTION AND HAZARD ANALYSIS State: December 15, 2004 WPF 5/0 Important milestones in the development of food safety systems Time Activity Distant past Use of prohibition principle
More informationFimbriae, Fibrils, Sex and Fuzzy Coats
Fimbriae, Fibrils, Sex and Fuzzy Coats The Limitation of Light One of the frustrating aspects of working with bacteria is that they are so small that it is almost impossible to see anything other than
More informationClinical Trials. Olli Saarela. September 18, Dalla Lana School of Public Health University of Toronto.
Introduction to Dalla Lana School of Public Health University of Toronto olli.saarela@utoronto.ca September 18, 2014 38-1 : a review 38-2 Evidence Ideal: to advance the knowledge-base of clinical medicine,
More informationWater Microbiology. Bacterial Pathogens and Water
Int. J. Environ. Res. Public Health 2010, 7, 3657-3703; doi:10.3390/ijerph7103657 Review Water Microbiology. Bacterial Pathogens and Water João P. S. Cabral Center for Interdisciplinary Marine and Environmental
More informationC. elegans as an in vivo model to decipher microbial virulence. Centre d Immunologie de Marseille-Luminy
C. elegans as an in vivo model to decipher microbial virulence Centre d Immunologie de Marseille-Luminy C. elegans : a model organism Mechanisms of apoptosis, RNA interference Neuronal function and development
More informationDr. habil. Anna Salek. Mikrobiologist Biotechnologist Research Associate
Dr. habil. Anna Salek Mikrobiologist Biotechnologist Research Associate BIOTECHNOLOGY of Food Science Cell Biology of Microorganisms Physiology of Microorganisms Biochemistry of Microorganisms Molecularbiology
More informationPhylogenetic background of enterotoxigenic and enteroinvasive Escherichia coli from patients with diarrhea in Sirjan, Iran
Volume 8 Number 3 (June 2016) 187-192 ORIGINAL ARTICLE Phylogenetic background of enterotoxigenic and enteroinvasive Escherichia coli from patients with diarrhea in Sirjan, Iran Taifeh Hoseinzadeh 1, Reza
More informationBacterial Virulence Strategies That Utilize Rho GTPases
CTMI (2005) 291:1 10 c Springer-Verlag 2005 Bacterial Virulence Strategies That Utilize Rho GTPases B. B. Finlay Biotechnology Laboratory, University of British Columbia, Vancouver British Columbia, V6T
More informationGenes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolates
JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2009, p. 2442 2451 Vol. 47, No. 8 0095-1137/09/$08.00 0 doi:10.1128/jcm.00566-09 Copyright 2009, American Society for Microbiology. All Rights Reserved. Genes Related
More informationBacterial Morphology and Structure م.م رنا مشعل
Bacterial Morphology and Structure م.م رنا مشعل SIZE OF BACTERIA Unit for measurement : Micron or micrometer, μm: 1μm=10-3 mm Size: Varies with kinds of bacteria, and also related to their age and external
More informationWHY IS THIS IMPORTANT?
CHAPTER 9 THE CLINICAL SIGNIFICANCE OF BACTERIAL ANATOMY WHY IS THIS IMPORTANT? Bacterial structures play a significant role in the five steps required for infection OVERVIEW The Clinical Signifcance of
More informationIntroduction to Microbiology BIOL 220 Summer Session I, 1996 Exam # 1
Name I. Multiple Choice (1 point each) Introduction to Microbiology BIOL 220 Summer Session I, 1996 Exam # 1 B 1. Which is possessed by eukaryotes but not by prokaryotes? A. Cell wall B. Distinct nucleus
More informationOCR Biology Checklist
Topic 1. Cell level systems Video: Eukaryotic and prokaryotic cells Compare the structure of animal and plant cells. Label typical and atypical prokaryotic cells. Compare prokaryotic and eukaryotic cells.
More informationOCR Biology Checklist
Topic 1. Cell level systems Video: Eukaryotic and prokaryotic cells Compare the structure of animal and plant cells. Label typical and atypical prokaryotic cells. Compare prokaryotic and eukaryotic cells.
More informationWorld Journal of Pharmaceutical Research SJIF Impact Factor 8.074
SJIF Impact Factor 8.074 Volume 7, Issue 5, 966-973. Research Article ISSN 2277 7105 MOLECULAR DETECTION OF ENTEROTOXIGENIC ISOLATES OF SALMONELLA TYPHIMURIUM, SHIGELLA FLEXNERI AND STAPHYLOCOCCUS AUREUS
More information3 S. Heidelberg ESBL Extended spectrum lactamase
Vol. 25 No. 123 almonella Heidelberg 1 almonella enterica serovar Heidelberg 1 3. Heidelberg EBL Extended spectrum lactamase CTX M 2 EBL. Heidelberg almonella enterica serovar Heidelberg 1 3. Heidelberg
More informationContains ribosomes attached to the endoplasmic reticulum. Genetic material consists of linear chromosomes. Diameter of the cell is 1 m
1. (a) Complete each box in the table, which compares a prokaryotic and a eukaryotic cell, with a tick if the statement is correct or a cross if it is incorrect. Prokaryotic cell Eukaryotic cell Contains
More information(A) Exotoxin (B) Endotoxin (C) Cilia (D) Flagella (E) Capsule. A. Incorrect! Only gram-positive bacteria secrete exotoxin.
College Biology - Problem Drill 13: Prokaryots and Protists Question No. 1 of 10 1. Gram-negative bacteria can cause disease in humans by release of what substance? Question #01 (A) Exotoxin (B) Endotoxin
More informationSupporting information
Electronic Supplementary Material (ESI) for Organic & Biomolecular Chemistry. This journal is The Royal Society of Chemistry 209 Supporting information Na 2 S promoted reduction of azides in water: Synthesis
More informationB1 REVISION CHAPTER 1 KEEPING HEALTHY
B1 REVISION CHAPTER 1 KEEPING HEALTHY What are the 7 components of a healthy diet? 1.. 2.. 3.. 4.. 5.. 6.. 7.. What are the different methods of infection? Describe the issues with being overweight Describe
More informationIntroduction to Bacteria
Introduction to Bacteria USDA NIFSI Food Safety in the Classroom University of Tennessee, Knoxville 2006 A quick clip http://www2.beavercreek.k12.oh.us/vi deos/28824/chp937402_700k.asf Bacteria What are
More informationMICROBIOLOGY (MICRO) Microbiology (MICRO) 1. MICRO 310: Medical Microbiology
Microbiology (MICRO) 1 MICROBIOLOGY (MICRO) Courses primarily for undergraduates: MICRO 101: Microbial World Prereq: High school biology or equivalent Introduction to the importance of viruses, bacteria,
More informationNatalie C. Marshall Finlay Lab University of British Columbia. How EPEC and EHEC modulate human cells via their type III secretion system
Natalie C. Marshall Finlay Lab University of British Columbia How EPEC and EHEC modulate human cells via their type III secretion system anthropomorphize verb (ăn thrə-pə-môr fīz ) : To attribute human
More informationVirulence of Enteropathogenic Escherichia coli, a Global Pathogen
CLINICAL MICROBIOLOGY REVIEWS, July 2003, p. 365 378 Vol. 16, No. 3 0893-8512/03/$08.00 0 DOI: 10.1128/CMR.16.3.365 378.2003 Copyright 2003, American Society for Microbiology. All Rights Reserved. Virulence
More informationAdvanced Herd Management Probabilities and distributions
Advanced Herd Management Probabilities and distributions Anders Ringgaard Kristensen Slide 1 Outline Probabilities Conditional probabilities Bayes theorem Distributions Discrete Continuous Distribution
More informationchapter one: the history of microbiology
chapter one: the history of microbiology Revised 6/19/2018 microbes microscopic (small) organisms, viruses, prions prefix sci. notation frac. equivalent dec. equivalent kilo- (k) 1 10 3 1000/1 = 1000 1000
More informationFate of Enterohemorrhagic Escherichia coli 0157:H7 in Apple Cider with and without Preservatives
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Aug. 1993, p. 2526-253 99-224/93/82526-5$2./ Copyright 1993, American Society for Microbiology Vol. 59, No. 8 Fate of Enterohemorrhagic Escherichia coli 157:H7 in
More informationProkaryotes & Viruses. Multiple Choice Review. Slide 1 / 47. Slide 2 / 47. Slide 3 / 47
New Jersey enter for Teaching and Learning Slide 1 / 47 Progressive Science Initiative This material is made freely available at www.njctl.org and is intended for the non-commercial use of students and
More informationProkaryotes & Viruses. Multiple Choice Review. Slide 2 / 47. Slide 1 / 47. Slide 3 (Answer) / 47. Slide 3 / 47. Slide 4 / 47. Slide 4 (Answer) / 47
Slide 1 / 47 Slide 2 / 47 New Jersey enter for Teaching and Learning Progressive Science Initiative This material is made freely available at www.njctl.org and is intended for the non-commercial use of
More informationANALYSIS OF MICROBIAL COMPETITION
ANALYSIS OF MICROBIAL COMPETITION Eric Pomper Microbiology 9 Pittsburgh Central Catholic High School Grade 9 Introduction Escherichia coli (E. coli) and Saccharomyces cerevisiae (Yeast) were grown together
More informationE. coli ETEC ETEC EPEC (AAEC) normal ETEC
E. coli normal Virotype Enterotoxic Enteroaggregative Enteropathogenic Enterohemorrhagic Enteroinvasive Necrotoxic Virulence factor pili,, LT, ST ST a,b bundle forming pili,, EAST effacing enteroadherence
More information