Dr. habil. Anna Salek. Mikrobiologist Biotechnologist Research Associate
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2 Dr. habil. Anna Salek Mikrobiologist Biotechnologist Research Associate
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4 BIOTECHNOLOGY of Food Science Cell Biology of Microorganisms Physiology of Microorganisms Biochemistry of Microorganisms Molecularbiology of Microorganisms Applied Microbiology
5 BIOTECHNOLOGY of Pharmaceutics Drug-protein R & D Regulatory Affairs for EU Development of vet. medicine Quality control Milecularbiology & Biochemistry Protein 5 Vet. medicine Parenteralia Microbiological Development of Drug-Proteinen Drug-Protein (Glykoprotein) Chemical Prophilaktic in Veterinary
6 Laboratory Experience (Experienced checking) Microbiology of Food Products (Predictive microbiology) Hygiene in Food Industry (HACCP) Microbiology in Environmental (Water & Soil & Air) Microbiology in Pharmaindustry & Veterinary med.
7 Dr. habil.. Anna Salek Microbiologist GERMANY, 2006
8 Germany September 2006 Dr. habil.. Anna Salek Microbiologist
9 Microorganisms surround us and affect our lives in many ways. They play key roles in processing whole biotechnology and affect the quality of our food, water, control some pests (biocontrol) and cause diseases.
10 Microbiological infections are back at the top of the public and political media with the thread of bioterrorism. How we should successfully contain and combat the threat and consequences of bioterrorist attack?
11 To meet this emerging threat, we have to address four important challenges:
12 1 st line of: Fully aware defence against bioterrorism weapons will be existing medical and public health response as well as efficient food chain surveillance system (i.e. HACCP).
13 2 nd line: Provide prophylaxis, medical care and infection control on massive scale in good network of onward diagnostic laboratory by enhancing capability to detect infections and conduct epidemiologic investigations.
14 3 rd line: Accelerating microbiological research and development of new drugs and vaccines as well as of rapid & safety diagnostics with application of recent attainment in science. 4 th line: Adequate investment to prepare Anti-Bioterrorism initiative.
15 The key areas of public health care Microbiological infections Chemical contamination Bioterrorism Epidemiology Chemical contamination Bioterrorism Epidemiology Microbiological infections
16 The key areas of public health care Microbiological infections Chemical contamination Bioterrorism Epidemiology Chemical contamination Bioterrorism Epidemiology Microbiological infections
17 Microbiological threat for public health Sources of Microbiological Infections Foodborne Pathogenes: - Fecal-oral transmission, - Gastroenteritis bacteria, - Food poisoning bacteria Infective Agents: - Human-to-Human, - Animal-to-Human, - Water & Air & Soil Bioterrorism: - Anthrax, Botulism, Plaque, Smallpox - VHF, FMD, - SARS?? Diagnostic: - Modern methods, i.e. FISH,PCR, ELISA, biosensors, - Classic biology methods Protecting the Food Supply and Production: - HACCP System, - Predictive Microbiology Diagnostic/Identification: - Rapid methods, - Sefety methods, - Onward methods Protecting Role of Food & Human Health Service: Cooperation between science, medical & veterinary service
18 Microbiological threat for public health Sources of Microbiological Infections Foodborne Pathogenes: - Fecal-oral transmission, - Gastroenteritis bacteria, - Food poisoning bacteria Infective Agents: - Human-to-Human, - Animal-to-Human, - Water & Air & Soil Bioterrorism: - Anthrax, Botulism, Plaque, Smallpox - VHF, FMD, - SARS?? Diagnostic: - Modern methods, FISH, PCR, ELISA, biosensors - Classic biology methods Protecting the Food Supply and Production: - HACCP System, - Predictive Microbiology Diagnostic/Identification: - Rapid methods, - Sefety methods, - Onward methods Protecting Role of Food & Human Health Service: Cooperation between science, medical & veterinary service
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20 Microbiological threat for public health Sources of Microbiological Infections Foodborne Pathogenes Fecal-Oral Transmission Bacteria Gastroenteritis Bacteria Food Poisoning Bacteria Foodborne Listeriosis Foodborne Animal Protozoa Viruses & Foodborne Biohazards Staphylococcocal S. aureus S. epidermidis S. haemolyticus Vibrio spp. V.cholerae V.vulnificus Gram-positive Sporeforming Bacteria Listera spp. Listeraia monocytogenes Giardia lamblia cysts Cryptosporidium parvum Oocysts Rotavirus Hepatitis Norwak Prions (BSE) Salmonella spp. S. typhimurium S.enteritidis S.paratyphi A Yersinia spp. Y.enterocolica Y.kristensenii Y.intermedia C. perfringens C. botulinum B. cereus Shigella spp. S.dysenteriae S.flexneri S.boydii Campylobacter C.jejuni C.coli C.intestinalis Escherichia Escherichia coli (EHEC)
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26 Microbiological threat for public health Sources of Microbiological Infections Foodborne Pathogenes Diagnostic of pathogenes: - Modern of molecularbiology & immunology methods: FISH, PCR, ELISA, biosensors, - Classic microbiological methods Protecting the Food Production and Supply: - HACCP and Microbiological Criteria for Safety Food, -Predictive Microbiology for Microbial Modeling, - Indicators of Food Microbial Quality and Safety
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29 Microbiological threat for public health Sources of Microbiological Infections Foodborne Pathogenes: - Fecal-oral transmission, - Gastroenteritis bacteria, - Food poisoning bacteria Infective Agents: - Human-to-Human, - Animal-to-Human, - Water & Air & Soil Bioterrorism: - Anthrax, Botulism, Plaque, Smallpox - VHF, FMD, - SARS?? Diagnostic: - Classic methods, - Medern methods, i.e. FISH, PCR, ELISA Protecting the Food Supply and Production: - HACCP System, - Predictive Microbiology Diagnostic/Identification: - Rapid methods, - Sefety methods, - Onward methods Protecting Role of Food & Human Health Service: Cooperation between science, medical & veterinary service
30 Sources of Microbiological Infections Infective Agents Human-to-Human Animal-to-Human Environment All Human Pathogenes i.e. Vibrio cholerae Zoonotic Microorganisms Surface & Supply Water Air in Environment and for public health Soil in Environment and for utility Salmonella Campylobacter Clostridium Klebsiella Bacteria: Coliforms Enterococci Legionella Protozoa: Giardia Cryptosp. Toxoplas. Yeasts: Candida Trychosporin Bacteria: Sporeforming Viruses: Human & Animals Fungi: Aspergillus fumigatus Bacteria & Fungi Viruses & Prions BSE
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34 Microbiological threat for public health Microbiological threat for public health Sources of Microbiological Infections Foodborne Pathogenes: - Fecal-oral transmission, - Gastroenteritis bacteria, - Food poisoning bacteria Infective Agents: - Human-to-Human, - Animal-to-Human, - Water & Air & Soil Bioterrorism: - Anthrax, Botulism, Plaque, Smallpox - VHF, FMD, - SARS?? Diagnostic: - Modern biology methods: FISH, PCR, ELISA, biosensors, - Classic biological methods Protecting the Food Supply and Production: - HACCP System, - Predictive Microbiology Diagnostic/Identification: - Rapid methods, - Sefety methods, - Onward methods Protecting Role of Food & Human Health Service: Cooperation between science, medical & veterinary service
35 Bioterrorism: Anthrax, Botulism, Plaque, Smallpox - VHF, FMD, - SARS??
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37 Bioterrorism in the World Diagnostic/Identification: - Rapid methods, - Sefety methods, - Onward methods Protecting Role of Food & Health Service Cooperation with Veterinary Anthrax: Bacillus anthracis Botulism: Clostridium botulinum Plaque: Yersinia pestis Smallpox: genus Orthopoxviruses Viral Hemorrhagic Fever (VHF): Several Viruses Tularemia: Francisella tularensis
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40 1 Key microbiological needs for Public Health Care Providing frontline co-operation between specialists from epidemiological services and national reference laboratories about: Investigations and surveillance of diseases from foodborne pathogens, i.e. gastroenteritis, protozoa, Viruses, prions, like PrPres (BSE); An application of Predictive Microbiology and HACCP System to identify in food any indicators and source of zoonotic as well as biohazards organisms; A protection of food production and supply disruption by bioterrorism.
41 2 Key microbiological needs for Public Health Care Application of the HACCP (hazard analysis critical control point) System according to microbiological criteria for safety food: Choice of indicators for food microbial quality & safety, like: Coliforms, Enterococci, Coliphages, Fecal microorganisms; HACCP An application of Predictive Microbiology for microbial modelling.
42 3 Key microbiological needs for Public Health Care Organisation of permanent education and training (also international) of specialist from another services, i.e. the response to bioterrorism. Cooperation with international Societies (WHO, FAO, FDA).
43 4 Key microbiological needs for Public Health Care Development and transfer of onward, safety and rapid methods for competent microbiological diagnostic, considering genetic research, integration of microbial databases and microbial biodiversity. 5 Key microbiological needs for Public Health Care Competently organised structure of routine microbiological laboratory (GLP) for connections with industry (GMP) and public services (collaborative working for health).
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