Microchem Silliker Private Limited, A-513, Microchem House, TTC Industrial Area, Mahape, Navi Mumbai, Maharashtra

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1 Last Amended on Page 1 of 8 I. FOOD & AGRICULTURAL PRODUCTS 1. All type of Food and Agri. products Aerobic Plate Count IS: ISO: 4833 Part Yeast & Mould Count IS: ISO :2008 Coliform Count IS: 5401 (Part 2) MPN/g ISO-4831:2006 ISO-4832:2006 IS: 5401 (Part 1) 2002 E. coli IS: 5887 (Part 1) 1976 ISO-7251: MPN/g Detection of S. aureus IS: 5887 (Part 2) 1976 Enumeration of S. aureus IS: 5887 (Part 8) : Sec / ISO 6888 Part Mesophilic spore count Foods APHA 4th Edition 2001 (Chap 22)

2 Last Amended on Page 2 of 8 Flat Sour Spore Count Foods APHA 4th Edition 2001 (Chap 24, 25) Detection of Clostridium perfringens IS: 5887 (Part 4) : cfu/g Anaerobic spore count Foods APHA 4th Edition 2001 (Chap 23,26) Faecal Streptococci count IS: 5887 (Part 2) 1976 Detection of Vibrio cholerae IS: 5887 (Part 5) 1976 Detection of Vibrio parahaemolyticus IS: 5887 (Part 5) 1976 Detection of Heat Resistant Moulds Foods 4th Edition 2001 (Chap 21) Enumeration of Bacillus cereus IS: 5887 (Part 6) Enterobacteriaceae Health Protection Agency (2005) National Standard Method F-18 ISO

3 Last Amended on Page 3 of 8 Detection of Shigellaspp IS: 5887 (Part 7) 1999 Detection of Salmonellaspp IS: 5887 (Part 3) 1999 ISO: By VIDAS MC/SOP/MIC/15, Issue No.02, Issue Date: Detection of Listeria monocytogenes IS (Part 1) Animal Feed products Aerobic Plate Count IS: ISO: 4833 Part Yeast & Mould Count IS: ISO :2008 Coliform Count IS: 5401 (Part 2) MPN/g ISO-4831:2006 ISO-4832:2006 IS: 5401 (Part 1) E. coli IS: 5887 (Part 1) ISO-7251: MPN/g Detection of S. aureus IS: 5887 (Part 2)

4 Last Amended on Page 4 of 8 Enumeration of S. aureus IS: 5887 (Part 8) : Sec / ISO 6888 Part Mesophilic spore count Flat Sour Spore Count Detection of Clostridium perfringens Anaerobic spore count Faecal Streptococci count Detection of Vibrio cholerae Detection of Vibrio parahaemolyticus Detection of Heat Resistant Moulds Foods - APHA 4th Edition 2001 (Chap - 22) Foods - APHA 4th Edition 2001 (Chap - 24, 25) IS: 5887 (Part 4) : 1999 Foods - APHA 4th Edition 2001 (Chap - 23,26) IS: 5887 (Part 2) IS: 5887 (Part 5) IS: 5887 (Part 5) Foods - 4th Edition 2001 (Chap - 21) cfu/g

5 Last Amended on Page 5 of 8 Enumeration of Bacillus cereus IS: 5887 (Part 6) Enterobacteriaceae Health Protection Agency (2005) National Standard Method F-18 ISO Detection of Shigellaspp IS: 5887 (Part 7) Detection of Salmonellaspp IS: 5887 (Part 3) ISO: By VIDAS MC/SOP/MIC/15,Issue No.02, Issue Date: II. WATER 1. WATER FROM ANY SOURCE (Drinking, Domestic, Irrigation, Packaged, Process, Mineral, Distilled, Effluent Water, Water used in Processed Food Water, any other water source) Detection of Listeria monocytogenes IS (Part 1) 2001 Aerobic Plate Count IS: (Reaffirmed 2009) cfu/ml IS: Coliform Count IS: (Reaffirmed 2009) IS: 5401 (Part 1) IS: cfu/ml 1 MPN /100ml 2 MPN /100ml cfu/ml E. coli IS: (Reaffirmed 2009) IS: 5887 (Part 1)

6 Last Amended on Page 6 of 8 Proteolytic organisms IS (Reaffirmed 2009) cfu/ml Lipolytic organisms IS (Reaffirmed 2009) cfu/ml Thermophilic Bacteria Count Sulphite Reducing Anaerobes IS (Reaffirmed 2009) Annexure C, IS: cfu/ml Pseudomonas aeruginosa Annexure D, IS: IS: Yeast & Mould Count IS: cfu/ml Salmonellaspp IS: Shigellaspp IS: 5887 (Part 7) Faecal Streptococci IS: 5887 (Part 2) IS: Staphylococcus aureus IS: 5887 (Part 2) Vibrio parahaemolyticus IS: 5887 (Part 5) MPN /100ml 2 MPN /100ml cfu/ml

7 Last Amended on Page 7 of 8 Vibrio cholerae IS: 5887 (Part 5) MS2 Phage USEPA method 1602 Cryptosporidium ISO : 2006 Giardia ISO : 2006 III. ENVIRONMENT & POLLUTION 1. Food and Other Industrial environmental monitoring Air in factory locations, units, storehouses, dairies, kitchens, any food handling locations Air Sampling (i) Bacterial Count (ii) Yeast & Mould Count MC/SOP/MIC/11,Issue No.02,Issue Date: (based on ISO 7218) cfu/min cfu/plate 2. Food and Packaging Industries - environmental monitoring Sanitization efficiency of equipments, handlers, linen, cooking platforms, cooking accessories Swabs (i) Bacterial Count (ii) Yeast & Mould Count (iii) Coliform Count (iv) E.coli count MC/SOP/MIC/06,Issue No.01, Issue Date: cfu/swab 3. Antiseptics and Disinfectants: 1. Soap Bars 2. Liquid Soap Antimicrobial efficacy testing MC/SOP/MIC/17,Issue No.02, Issue Date: (based on BS EN 1040:2005) Log reduction:3-5 for acceptability

8 Last Amended on Page 8 of 8 IV MOLECULAR ANALYSIS GMO TESTING 1 Soya & Soya based products Qualitative PCR test for 35 S promoter and NOS terminator. MC/SOP/GMO/03, Issue No.02, Issue Date: (based on IS/ISO 21569:2005, Reaffirmed ) Qualitative,, LOD 0.1% 2 Cotton and Cotton based products Qualitative PCR test for 35 S promoter and NOS terminator. MC/SOP/GMO/04, Issue No.02, Issue Date: (based on IS/ISO 21569:2005, Reaffirmed ) Qualitative,, LOD 0.1% 3 Maize and Maize based product Qualitative PCR test for 35 S promoter and NOS terminator. MC/SOP/GMO/05, Issue No.02, Issue Date: (based on IS/ISO 21569:2005, Reaffirmed ) Qualitative,, LOD 0.1% -X-X-X-X-X-X-X-X-X-X-X-X-X-X-X-X- *NOTE: The Laboratory has demonstrated competence for the stated scope for WATER. This however does not fully cover the specification requirements of BIS for the Packaged Drinking Water as per IS:14543 and the Packaged Natural Mineral Water IS:13428.