Microbiological Testing Summary
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1 BACTERICIDAL ACTIVITY EN Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas Test method and requirements (phase 2, step 1) Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Enterococcus hirae Clean conditions (0.3g/l bovine albumin) - Dirty conditions (3.0g/l bovine albumin) Obligatory Log reduction 5 in 5 minutes at 20 C Acinetobacter calcoaceticus ABBOTT ANALYTICAL 1:30 EN1276 Dirty > LOG 5 Enterococcus hirae ABBOTT ANALYTICAL 1:100 EN1276 Dirty > LOG 5 Enterococcus hirae BLUTEST 1:100 EN1276 Dirty > LOG 5 Escherichia coli ABBOTT ANALYTICAL 1:100 EN1276 Dirty > LOG 5 Escherichia coli BLUTEST 1:100 EN1276 Dirty > LOG 5 Listeria monocytogenes ABBOTT ANALYTICAL 1:100 EN1276 Dirty > LOG 5 Methicillin -resistant Staphylococcus aureus ABBOTT ANALYTICAL 1:100 EN1276 Dirty > LOG 5 Methicillin-resistant Staphylococcus pseudintermedius ABBOTT ANALYTICAL 1:100 EN1276 Dirty > LOG 5 Pseudomonas aeruginosa ABBOTT ANALYTICAL 1:100 EN1276 Dirty > LOG 5 Pseudomonas aeruginosa BLUTEST 1:100 EN1276 Dirty > LOG 5 Salmonella tryphimurium ABBOTT ANALYTICAL 1:100 EN1276 Dirty > LOG 5 Staphylococcus aureus ABBOTT ANALYTICAL 1:100 EN1276 Dirty > LOG 5 Staphylococcus aureus BLUTEST 1:100 EN1276 Dirty > LOG 5 Streptococcus equi ABBOTT ANALYTICAL 1:100 EN1276 Dirty > LOG 5 EN Chemical disinfectants and antiseptics Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas Test method and requirements without mechanical action (phase 2, step 2) BACTERIA - Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Enterococcus hirae FUNGI - Clean conditions (0.3g/l bovine albumin)- Dirty conditions (3.0g/l bovine albumin) Obligatory Bacteria - Log reduction 4 in 5 minutes at 20 C, FUNGI Log reduction 3 in 15 minutes at 20 C Enterococcus hirae BLUTEST 1:100 EN13697 Dirty > LOG 5 Escherichia coli BLUTEST 1:100 EN13697 Dirty > LOG 5 Pseudomonas aeruginosa BLUTEST 1:100 EN13697 Dirty > LOG 5 Staphylococcus aureus BLUTEST 1:100 EN13697 Dirty > LOG 5
2 BACTERICIDAL ACTIVITY (cont.) EN Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area Test method and requirements (phase 2, step 1) Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus, Enterococcus hirae Low-level soiling (3g/l bovine albumin) - High-level soiling (10g/l bovine albumin + 10g/l yeast extract) Obligatory Log reduction 5 in 30 minutes at 10 C Bordetella bronchiseptica ABBOTT ANALYTICAL 1:100 EN1656 Dirty > LOG 5 Campylobacter jejuni ABBOTT ANALYTICAL 1:100 EN1656 Dirty > LOG 5 Methicillin-resistant Staphylococcus pseudintermedius ABBOTT ANALYTICAL 1:100 EN1656 Dirty > LOG 5 Rhodococcus equi ABBOTT ANALYTICAL 1:100 EN1656 Dirty > LOG 5 Streptococcus equi ABBOTT ANALYTICAL 1:100 EN1656 Dirty > LOG 5 EN 13623, Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of bactericidal activity against Legionella pneumophila of chemical disinfectants for aqueous systems Test method and requirements (phase 2, step 1) Obligatory Log reduction 5 in 60 minutes at 30 C Legionella pneumophila ABBOTT ANALYTICAL 1:100 EN13623 > LOG 5 HLD4 formulation has also been tested under EN1275 and EN1040 EN Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics Test method and requirements (phase 1) Pseudomonas aeruginosa, Staphylococcus aureus Obligatory Lg reduction 5 in 5 minutes at 20 C EN Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics Test method and requirements (phase 1) Obligatory Lg reduction 4 in 15 minutes at 20 C
3 VIRUCIDAL ACTIVITY BS EN 14476: Chemical disinfectants and antiseptics. Virucidal quantitative suspension test for chemical disinfectants and antiseptics used in human medicine. Test method and requirements (phase 2, step 1) Adenovirus and Poliovirus Obligatory Lg reduction 4 (Requirements may vary by claim) Adenovirus BLUTEST 1:100 EN14476 DIRTY LOG 3.67 Canine Parvovirus (CPV2) BLUTEST 1:100 EN14476 CLEAN > LOG 4 Canine Parvovirus (CPV2) BLUTEST 1:50 EN14476 DIRTY > LOG 4 Feline Infectious Peritonitis ATS 1:50 EPA DIRTY > LOG 4 Hepatitis B ATS LABS 1:50 EN14476 CLEAN LOG 4.67 Herpes Simplex BLUTEST 1:50 EN14476 DIRTY LOG 4.16 Human Immunodeficiency Virus BLUTEST 1:50 EN14476 DIRTY LOG 4.50 Norovirus (Feline Calicivirus) BLUTEST 1:50 EN14476 DIRTY LOG 4.00 SPORICIDAL ACTIVITY EN Chemical disinfectants and antiseptics Basic sporicidal activity Test method and requirements (phase 1) Bacillus subtilis, Bacillus cereus Obligatory Lg reduction 3 in 60 minutes at 20 C Bacillus cereus ABBOTT ANALYTICAL 1:50 (10 deg C) EN14347 LOG 3.18 Bacillus subtilis ABBOTT ANALYTICAL 1:50 (10 deg C) EN14347 LOG 3.17 EN Chemical disinfectants Quantitative suspension test for the evaluation of sporicidal activity of chemical disinfectants used in human medicine, veterinary field, and food, industrial, domestic and institutional areas Test method and requirements (phase 2, step 1) Bacillus subtilis, Bacillus cereus Clean conditions (0.3g/l bovine albumin)- Dirty conditions (3.0g/l bovine albumin + 3.0ml/l sheep erythrocytes) Obligatory Lg reduction 3 in 60 minutes at 20 C Clostridium difficile ABBOTT ANALYTICAL 1:10 (10 deg C) EN13704 DIRTY > LOG 3
4 MYCOBACTERICIDAL ACTIVITY EN Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of mycobactericidal activity of chemical disinfectants and antiseptics used in the veterinary area Test method and requirements (phase 2, step 1) Mycobacterium avium Low-level soiling (3g/l bovine albumin)- High-level soiling (10g/l bovine albumin + 10g/l yeast extract) Obligatory Lg reduction 4 in 60 minutes at 10 C Mycobacterium avium ABBOTT ANALYTICAL 1:50 EN14204 DIRTY > LOG 4 Mycobacterium bovis ABBOTT ANALYTICAL 1:50 EN14204 DIRTY > LOG 4 Mycobacterium terrae ABBOTT ANALYTICAL 1:50 EN14204 DIRTY > LOG 4 EN Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of mycobactericidal activity of chemical disinfectants in the medical area including instrument disinfectants Test method and requirements (phase 2, step 1) Mycobacterium avium, Mycobacterium terrae Clean conditions (0.3g/l bovine albumin)- Dirty conditions (3.0g/l bovine albumin + 3.0ml/l sheep erythrocytes) Obligatory Lg reduction 4 in 60 minutes at 20 C Mycobacterium avium CHEMILA 1:20 EN14348 CLEAN > LOG 4 Mycobacterium avium CHEMILA 1:20 EN14348 DIRTY > LOG 4 Mycobacterium terrae CHEMILA 1:20 EN14348 CLEAN > LOG 4 Mycobacterium terrae CHEMILA 1:20 EN14348 DIRTY > LOG 4 FUNGICIDAL ACTIVITY EN Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas Test method and requirements (phase 2, step 1) Clean conditions (0.3g/l bovine albumin)- Dirty conditions (3.0g/l bovine albumin) Obligatory Lg reduction 4 in 15 minutes at 20 C Aspergillus niger ABBOTT ANALYTICAL 1:100 (10 deg C) EN1650 DIRTY > LOG 4 Candida albicans ABBOTT ANALYTICAL 1:100 (10 deg C) EN1650 DIRTY > LOG 4
5 FUNGICIDAL ACTIVITY (cont.) EN Chemical disinfectants and antiseptics Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas Test method and requirements without mechanical action (phase 2, step 2) Clean conditions (0.3g/l bovine albumin) - Dirty conditions (3.0g/l bovine albumin) Fungicidal/Yeasticidal Obligatory Lg reduction 3 in 15 minutes at 20 C Candida albicans BLUTEST 1:50 EN13697 DIRTY > LOG 4 Candida albicans BLUTEST 1:100 EN13697 DIRTY > LOG 4 DNA / RNA HLD4 formulation is proven to denature / precipitate DNA / RNA immediately at dilutions of 1:20 or 1:50 according to Cambridge University Technical Services Further to your query regarding the efficacy of Medimark Scientific Limited s product HLD4, CUTS can report that at dilutions of HLD4 less than 1:100 (v/v) 1:20 or 1:50 for example nucleic acids are precipitated immediately. For such dilutions (less than or equal to 1:100), the contact time for immersed solutions should be a few minutes at maximum. The product was not tested in aerosol form. However, it is likely that if the concentrations of HLD4 in an aerosol formulation is similar and the active ingredients are stable that it would behave similarly. Concentrate Spray (1:20) Wipes (1:20) XTM305 XTM309 XTM308 XTM301 XTM302 XTM353 FOR MORE INFORMATION AND A COMPETITIVE QUOTE, CONTACT YOUR LOCAL STARLAB ACCOUNT MANAGER STARLAB (UK), LTD 5 Tanners Drive, Blakelands, Milton Keynes MK14 5BU. Tel: Fax: infoline@starlab.co.uk
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Consulting Scientists to the Disinfectant Industry. Test Report. Sample ref: 16G/027 Date received: 12 July 2016
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