MICROBIOLOGICAL TESTING DETAILS. S. No. Test Description Test Method 01 HPC APHA 9215 B. 02 Total Coliforms by Membrane Filtration APHA 9222 B
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1 MICROBIOLOGICAL TESTING DETAILS WATER SAMPLE ANALYSIS DETAILS: 1. DRINKING WATER OR POTABLE WATER OR UNBOTTLED WATER: 02 Total Coliforms by Membrane Filtration APHA 9222 B 03 Fecal Coliforms by Membrane APHA 9222 B & G 04 E. Coli by Membrane Filtration APHA 9222 B & G 05 Fecal Streptococci or Enterococci APHA 9230 C 06 Pseudomonas aeruginosa ISO Staphylococcus aureus NSM W10 08 Sulphite Reducing Clostridia CCFRA Yeast and Moulds APHA 9610 D 2. TAP WATER/TANK WATER/WATER FROM WATER TANKERS: 02 Legionella ISO BOTTLED WATER (AVAILABLE IN PACKED BOTTLES FROM DIFFERENT BRANDS): 01 Total Coliforms by Membrane Filtration APHA 9222 B 02 Fecal Coliforms by Membrane APHA 9222 B & G 03 E. Coli by Membrane Filtration APHA 9222 B & G 04 Fecal Streptococci or Enterococci APHA 9230 C 05 Pseudomonas aeruginosa ISO Sulphite Reducing Clostridia CCFRA 2.8.3
2 4. COOLING WATER SYSTEM (COOLING TOWERS/EVAPORATIVE CONDENSORS): 02 Legionella ISO HOT WATER SYSTEM & COLD WATER SYSTEM: 02 Legionella ISO WATER SYSTEM: (SHOWER HEAD/FOUNTAIN/SPA/JACUZZI/AIR WASHERS/HUMIDIFIERS): 02 Legionella ISO DENTAL CHAIR WATER (HANDPIECE/CUPFILLER): 02 Legionella ISO EDIBLE PACKAGED ICE: 02 Total Coliforms by Membrane Filtration APHA 9222 B 03 Fecal Coliforms by Membrane APHA 9222 B & G 04 E. Coli by Membrane Filtration APHA 9222 B & G 05 Pseudomonas aeruginosa ISO 16266
3 9. SWIMMING POOL WATER: 02 Total Coliforms by Membrane Filtration APHA 9222 B 03 Fecal Coliforms by Membrane APHA 9222 B & G 04 Fecal Streptococci or Enterococci APHA 9230 C 05 Pseudomonas aeruginosa ISO RAW WATER OR NON POTABLE WATER OR SURFACE WATER (INDUSTRIAL WASTE WATER): 01 Fecal Coliforms by MPN APHA 9221 E 02 Nematological Examination APHA B 11. RAW WATER OR NON POTABLE WATER OR SURFACE WATER (IRRIGATION WATER): 01 Fecal Coliforms by MPN APHA 9221 E 12. RAW WATER OR NON POTABLE WATER OR SURFACE WATER (GROUND WATER): 01 Fecal Coliforms by MPN APHA 9221 E 13. RAW WATER OR NON POTABLE WATER OR SURFACE WATER (MARINE WATER): 01 E.coli by MPN APHA 9221 F
4 14. RAW WATER OR NON POTABLE WATER OR SURFACE WATER (WASTEWATER DISPOSAL TO MARINE WATER): 01 Coliforms by MPN APHA 9221 B 02 Fecal Coliforms by MPN APHA 9221 E 03 Egg & Warm Parasites (I.E, *Nematological Examination) APHA B 15. RAW WATER OR NON POTABLE WATER OR SURFACE WATER (WASTEWATER DISPOSAL TO SEA WATER): 01 Fecal Coliforms by MPN APHA 9221 E NOTE: *NEMATOLOGICAL EXAMINATION - APHA B COSMETICS SAMPLE ANALYSIS DETAILS: 1. COSMETICS (CREAM/LOTION/SUSPENSION/SOLUTION/OINTMENT/POWDER/GEL/STICK): 01 Enumeration and detection of AerobicMesophilic Bacteria ISO Detection of Staphylococcus aureus ISO Detection of Pseudonomas aeruginosa ISO Detection of E.coli ISO Enumeration of Yeast & Mould ISO AIR (ROOM): AIR SAMPLE ANALYSIS DETAILS: 01 Total Bacterial Count 02 Fungal Count NMAM 800
5 SWAB ANALYSIS DETAILS: 1. HAND SWAB (PERSONNEL): 01 Total Bacterial Count BAM Chapter 3 02 Total Coliforms CCFRA Staphylococcus aureus CCFRA SURFACE SWAB ANALYSIS DETAILS: 1. SURFACE SWAB - UTENSIL (SPOON/KNIFE/BOWL/CUP/PLATE/ETC): 01 Total Bacterial Count BAM Chapter 3 02 Total Coliforms CCFRA E.coli BAM Chapter 4 2. SURFACE SWAB - SURFACE (CHOPPING BOARD/TABLE/MOLD/ETC): 01 Total Bacterial Count BAM Chapter 3 02 Total Coliforms CCFRA E.coli BAM Chapter 4 3. SURFACE SWAB - EQUIPMENTS (CONVEYOR BELT/X-RAY MACHINE/CHILLER/ETC): 01 Total Bacterial Count BAM Chapter 3 02 Total Coliforms CCFRA E.coli BAM Chapter 4
6 FOOD ANALYSIS DETAILS: 1. DAIRY PRODUCTS (MILK/CREAM): 02 Enumeration of Enterobacteriaceae CCFRA E.coli by MPN method CMMEF Coliforms by MPN method CMMEF Salmonella sps CCFRA Yeast and Moulds CCFRA Staphylococcus Aureus CCFRA DAIRY PRODUCTS (CHEESE): 01 Enumeration of Enterobacteriaceae CCFRA E.coli by MPN method CMMEF Salmonella sps CCFRA Staphylococcus aureus CCFRA Listeria spp. Reveal test kit, Neogen Corporation 06 Aerobic Plate Count BAM Chapter 3 3. DAIRY PRODUCTS (ICE-CREAM): 01 Enumeration of Enterobacteriaceae CCFRA E.coli by MPN method CMMEF Salmonella sps CCFRA Staphylococcus aureus CCFRA Yeast and Moulds CCFRA Aerobic Plate Count BAM Chapter 3
7 4. DIETIC FOODS (BISCUITS): 01 Enumeration of Enterobacteriaceae CCFRA Yeast and Moulds CCFRA Salmonella sps CCFRA Staphylococcus aureus CCFRA E. Coli O157 : H7 AOAC Bacillus Cereus BAM Chapter DIETIC FOODS (CEREAL BASED FOODS): 02 Salmonella sps CCFRA Staphylococcus aureus CCFRA Bacillus Cereus BAM Chapter Listeria spp. Reveal test kit, Neogen Corporation 06 Clostridium Perfringens CCFRA Enumeration of Enterobacteriaceae CCFRA E. Coli O157 : H7 AOAC MEAT,POULTRY AND ITS PRODUCTS: 02 Salmonella sps CCFRA E. Coli O157 : H7 AOAC Clostridium Perfringens CCFRA Staphylococcus aureus CCFRA Enumeration of Enterobacteriaceae CCFRA Listeria spp. Reveal test kit, Neogen Corporation
8 7. FISH,SHELLFISH AND THEIR PRODUCTS: 02 E.coli by MPN method CMMEF Vibrio Parahaemolyticus CCFRA Salmonella sps CCFRA Listeria spp. Reveal test kit, Neogen Corporation 06 Staphylococcus aureus CCFRA E. Coli O157 : H7 AOAC Clostridium Perfringens CCFRA *SHIGELLA SPS. BAM CHAPTER 6 8. EGG AND THEIR PRODUCTS: 02 E.coli by MPN method CMMEF Enumeration of Enterobacteriaceae CCFRA Salmonella sps CCFRA Listeria spp. Reveal test kit, Neogen Corporation 06 Staphylococcus aureus CCFRA Bacillus Cereus BAM Chapter Clostridium Perfringens CCFRA TOMATO CONCENTRATES: 02 Yeast and Moulds CCFRA Salmonella sps CCFRA 3.1.2
9 10. FATS AND OILS: 01 Enumeration of Enterobacteriaceae CCFRA Salmonella sps CCFRA Listeria spp. Reveal test kit, Neogen Corporation 04 Staphylococcus aureus CCFRA Yeast and Moulds CCFRA E.coli by MPN method CMMEF Aerobic Plate Count BAM Chapter SPICES AND HERBS: 02 Yeast and Moulds CCFRA Staphylococcus aureus CCFRA Bacillus Cereus BAM Chapter Clostridium Perfringens CCFRA Salmonella sps CCFRA Fecal Coliforms by MPN CMMEF SAUCES: 02 Yeast and Moulds CCFRA Enumeration of Enterobacteriaceae CCFRA E.coli by MPN method CMMEF Salmonella sps CCFRA Staphylococcus aureus CCFRA 3.5.1
10 13. CEREALS: 02 Yeast and Moulds CCFRA Staphylococcus aureus CCFRA Bacillus Cereus BAM Chapter Clostridium Perfringens CCFRA Salmonella sps CCFRA E.coli by MPN method CMMEF Coliforms by MPN method CMMEF Enumeration of Enterobacteriaceae Reveal test kit, Neogen Corporation 10 Listeria spp. CCFRA FRUITS AND VEGETABLES: 01 Yeast and Moulds CCFRA E.coli by MPN method CMMEF Salmonella sps CCFRA Staphylococcus aureus CCFRA Listeria spp. Reveal test kit, Neogen Corporation 06 E. Coli O157 : H7 AOAC Bacillus Cereus BAM Chapter Clostridium Perfringens CCFRA JAM AND JELLY: 01 Yeast and Moulds CCFRA Salmonella sps CCFRA 3.1.2
11 16. VINEGAR: 17. CHOCOLATES,SWEETS AND THEIR INGREDIENTS: 02 Yeast and Moulds CCFRA Enumeration of Enterobacteriaceae CCFRA Salmonella sps CCFRA Staphylococcus aureus CCFRA Listeria spp. Reveal test kit, Neogen Corporation 07 E. Coli O157 : H7 AOAC INGREDIENTS FOR FOOD INDUSTRY: 02 Salmonella sps CCFRA Staphylococcus aureus CCFRA Enumeration of Enterobacteriaceae CCFRA Clostridium Perfringens CCFRA BEVERAGES: 02 Salmonella sps CCFRA Staphylococcus aureus CCFRA Yeast and Moulds CCFRA Bacillus Cereus BAM Chapter E.coli by MPN method CMMEF Coliforms by MPN method CMMEF E. Coli O157 : H7 AOAC
12 20. READY TO EAT FOODS: 01 E.coli by MPN method CMMEF E. Coli O157 : H7 AOAC Salmonella sps CCFRA Listeria spp. Reveal test kit, Neogen Corporation 05 Staphylococcus aureus CCFRA Bacillus Cereus BAM Chapter Enumeration of Enterobacteriaceae CCFRA Clostridium Perfringens CCFRA * NOTE: In food, parameters varies as per different food products.
Microchem Silliker Private Limited, A-513, Microchem House, TTC Industrial Area, Mahape, Navi Mumbai, Maharashtra
Last Amended on 28.03.2014 Page 1 of 8 I. FOOD & AGRICULTURAL PRODUCTS 1. All type of Food and Agri. products Aerobic Plate Count IS: 5402 2012 ISO: 4833 Part 1 2013 Yeast & Mould Count IS: 5403 1999 ISO-21527-2:2008
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