ID Membranes for Microbial Rapid Identification
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1 ID Membranes for Microbial Rapid Identification Chromogenic Reaction by Specific Substrates on Membranes
2 Classical plates are still used today as first steps in modern microbiology. Smart, inexpensive membranes can help to identify organisms within 1 to 4 hours. These membranes are for economical and rapid identification and confirmation of microorganisms in water, food, environmental and clinical samples. They find their application in various sectors in food and dairy industry, water industry, pharmaceutical laboratory testing, cosmetic industry, environmental and sanitary testing, clinical diagnostic etc. After the routine inoculation and isolation technique the membranes enable the direct identification. Principle The membranes contains chromogenic substrates such as ONPG, X-Gal, or X-Glu and other substrates and inidcators which serve as the basis for the differentiation by color. The target organisms are characterized by enzyme systems that metabolize the substrates and initiate the color change. The colors can then be visually detected on the membranes and show the enzymatic activity of the microoganisms which is a helpful tool for the identification of genus and species. Figure 2: Placement of the membrane on an incubated plate with colonies. 3. Replication: For replication technique place the membrane on the surface of agar plate. Allow the membrane to adsorb the grown colonies. Perform this step for maximum of 30 seconds to 1 minute. Mark the corresponding orientation of paper. Method Workflow 1. Inoculation and Isolation: Inoculate the organisms from sample on any of general purpose media, nutrient agar, tryptic soy agar, plate count agar etc. Adopt any of surface plating methods as; spread plate method, quadrant (four or five) streak pattern or T streak method so as to obtain isolated colonies from inoculums. 2. Incubation: Incubate at C for hours. Figure 3: Replication of the membrane. 4. Identification: Incubate the replicated identification membrane in empty sterile Petri dish at C for 1 4 hours or if desired membrane can be placed on dry lid of same plate and incubate in inverted position (*if lid has moisture wiped it with sterile cotton). Alternately the membrane may be kept for incubation on growth media at C. Observe for development of color and interpret results. For further reference the membranes can be autoclaved and then be stored or may be scanned and saved on a computer. Specifications: Membranes are sterile and have a diameter of 70 mm. Storage Temperature: 2 8 C (in the dark). Figure 1: The membrane is may marked for the orientation of the paper. The membrane is removed from the box with sterile forceps under aseptic conditions. 2 sigma-aldrich.com/idmembranes
3 78039 Bacillus ID Membrane For rapid detection and differentiation between various species of Bacillus such as B. subtilis, B. cereus, B. thuriangiensis, B. megaterium, B. coagulans, B. pumilus from food, meat, fish, cosmetic and pharmaceutical preparations. Appearance: Pale pink colored membrane. Bacillus cereus (10876) Bacillus subtilis (6633) Bacillus thuringiensis (10876) Biochemical Esculin ID Membrane (Esculin Test ID Membrane) Enterococcus feacalis (29212) Enterococcus feacium (27273) Light blue Yellowish green to green Light blue Appearance: Light brown colored membrane. For rapid detection of Group D streptococci from food, dairy, water samples and pharmaceutical products etc. Black Black Bacillus subtilis (ATCC 6633) Green colored Enterococcus feacalis (ATCC 29212) Black colored Differential Coli-E. coli ID Membrane For rapid detection of E. coli, Klebsiella, Pseudomonas and Salmonella species in food and environmental samples. Klebsiella pneumoniae (13883) Salmonella Enteritidis (13076) Dark blue to violet Light pink Salmon to red, greenish, pigment may be observed Light pink colored Dark blue to violet colored Salmonella Enteritidis (ATCC 13076) Salmon to red colored Differential ID Membrane For rapid differentiation of Lactose fermenting and Lactose non fermenting enteric bacteria from water, food, dairy products, cosmetics, pharmaceutical preparations etc. Appearance: Pinkish colored membrane. Escherichia coli (8739) Dark pink Dark pink Dark pink colored Salmonella Typhimurium (ATCC 14028) 3
4 73257 DNase ID Membrane For rapid detection of deoxyribonuclease (DNA) activity of bacteria especially for identification of pathogenic staphylococci. Appearance: Blue colored membrane Dual Confirmation of E. coli ID Membrane Pink zone around the colony, DNase positive Fluorescence under UV light Negative Blue Positive Negative Negative Pink zone For rapid detection and confirmation of Escherichia coli in water and food samples, based on a chromogenic and fluorogenic methods. Fluorescence under UV (365nm) Escherichia coli ATCC Blue color E. coli Chromogenic ID Membrane For rapid detection and confirmation of Escherichia coli in water and food samples. Appearance: White colored membrane Blue Blue color 4 sigma-aldrich.com/idmembranes
5 06722 E. coli Fluorogenic ID Membrane For rapid detection and confirmation of Escherichia coli in water and food samples on the basis of fluorogenic emission at 365 nm. Fluorescence under UV light Negative Positive Negative Negative Pseudomonas ID Membrane For rapid detection of Pseudomonas aeruginosa from clinical and nonclinical specimens. Fluorescence under UV light Negative Positive Enterococcus feacalis (29212) Negative Klebsiella pneumoniae (13883), mucoid Negative Fluorescence under UV (365nm) Escherichia coli ATCC Fluorescence under UV (365nm) Fluorescence colored Blue colored no fluorescence Salmonella ID Membrane For rapid detection of Salmonella species from coliforms. Salmonella Enteritidis (13076) Klebsiella pneumoniae (13883) Blue Light purple Light purple, mucoid Salmonella Typhimurium (ATCC 14028) Light purple colored Salmonella Enteritidis (ATCC 13076) Light purple colored mucoid Blue colored Total Coliform ID Membrane For qualitative detection of coliforms from water, pharmaceutical preparations, dairy and food products. Appearance: Pink colored membrane. Enterobacter cloacae (23355) Citrobacter freundii (8090) Klebsiella pneumoniae (13883) Dark blue Salmon to red Salmon to red Light pink Dark blue colored Enterobacter cloacae (ATCC 23355) Salmon to red colored Light pink mucoid colored 5
6 39187 Universal Environmental ID Membrane For rapid detection of Pseudomonas, Enterococcus, E. coli and Salmonella species etc. from environmental samples, samples of clinical origin such as nosocomial samples. Appearance: Pale pink colored membrane. Enterococcus faecalis (29212) Universal Food pathogen ID Membrane Bacillus cereus (10876) Listeria monoyctogenes (19111) Escherichia coli O157:H7 (NCTC 12900) Shigella flexneri (12022) Pink-purple Golden yellow (greenish pigment is observed) Blue-blue green (small) Appearance: Light pink colored membrane. For rapid detection of food pathogens such as E. coli, E. coli O157:H7, Staphylococcus aureus, Salmonella, Bacillus, Listeria and Shigella species etc. from various food, dairy, fish, and meat products. Purple Golden yellow Light green (big) Blue-green Purple pink, greenish, pigment may be observed Pink to purple colored Enterococcus faecalis (ATCC 29212) Blue colored small Listeria monoyctogenes (ATCC 19111) Blue colored Purple colored Escherichia coli O157:H7 (NCTC 12900) Purple pink colored, greenish, pigment may be observed Universal Microbial Limit Test Membrane Recommended for detection of pathogenic microorganisms such as E. coli, S. aureus, P. aeruginosa, Bacillus and Salmonella species from pharmaceutical preparations, raw materials, cosmetic samples etc. Pseudomonas aeruginosa (9027) Escherichia coli (8739) Staphylococcus aureus (6538) Salmonella Abony (NCTC 6017) Pink-purple Green to bluish-green Escherichia coli (ATCC 8739) Pink-purple color may be observed Staphylococcus aureus (ATCC 6538) Green color may be observed, greenish, pigment may be observed 6 sigma-aldrich.com/idmembranes
7 30374 UTI ID Membrane (Urinary Tract Infections ID Membrane) For rapid detection and confirmation of microorganisms mainly causing urinary tract infection, for eg. E. coli, Proteus, Klebsiella, Pseudomonas, S. aureus, and Enterococcus species. Enterococcus faecalis (29212) Klebsiella pneumoniae (13883) Proteus mirabilis (12453) Carbohydrate Fermentation Membranes Pink-purple Golden yellow Blue-blue green (small) Blue to purple mucoid Light brown Cat. No. Product Name Product Description Glucose Fermentation Membrane For rapid detection of Lactose Fermentation Membrane carbohydrate fermenting organisms from mixed Mannitol Fermentation Membrane flora where fermenting Sucrose Fermentation Membrane organisms will exhibit Xylose Fermentation Membrane yellow color. Appearance: Pinkish colored membrane., greenish, pigment may be observed Blue to purple colored, mucoid Pink to purple colored Enterococcus faecalis (ATCC 29212) Blue colored small Golden yellow Development of yellow color indicates positive color Glucose Lactose Mannitol Sucrose Xylose Citrobacter freundii (8090) Enterobacter aerogenes (13048) Klebsiella pneumoniae (13883) Proteus vulgaris (13315) Salmonella typhimurium (14028) Salmonella typhi (6539) Serratia marcescens (8100) Shigella flexneri (12022) + + 7
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