REFERENCE MATERIAL CATALOGUE. FOR PHYSICAL-CHEMICAL AND MICROBIOLOGICAL LABORATORIES (edition: February 2011)

Transcription

1 REFERENCE MATERIAL CATALOGUE FOR PHYSICAL-CHEMICAL AND MICROBIOLOGICAL LABORATORIES (edition: February 2011)

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3 ielab is a company engaged to provide services and products for the application of quality in testing laboratories, that offers the organization of Proficiency Testing Schemes on international basis or the manufacturing and sale of physical-chemical and microbiological reference materials, among others. The present catalogue is the result of the work that has been developed by our R+D+i Department and our Quality Department, that have as reference the quality, the independence and to give answer to the technological necessities that are arising in the exercise of our work. They also have given responses to the necessities that the application of the quality required and which until then did not exist. It is for the reason of this constant process of research, innovation and application to the quality that this catalogue is something dynamic and in a continuous change, it is a reflection of our offer only the day in which it was published. In addition, many of our materials are certified in the process of Proficiency Testing Schemes, reason why new materials with each closed round are included, becoming an updated catalogue impossible. For that reason we invite you to visit our Web page ( where you can find our latest catalogue and diverse information that sure will be of your interest. Trusting that our cooperation can contribute to the continuous improvement of the quality of your laboratory, we hope to count on your visit. 3

4 INDEX page Presentation 3 Index of contents 4 Physical-chemical reference material Properties of the physical-chemical reference material 5 Advantages of the physical-chemical reference material 5 Wastewaters 6 Drinking waters 6 Sludges 6 Sample supports 6 Sea waters 6 Soils 6 Absorption solution 6 PCBs in dielectric oils 6 Microbiological reference material Properties of the microbiological reference material 7 Advantages of the microbiological reference material 7 BAControl 8 BACuanti 16 BACuali 20 Materials for molecular biology (PCR and DNA) 22 Price list Reference material price list 24 Reference material purchase order form 25 4

5 Physical-chemical reference material ielab presents a wide range of reference materials that have been specially designed to make easy all the quality control tasks accomplished in diagnostic laboratories. All the supplied material comes accompanied by the corresponding Certificate of Analysis and User Guide. Properties of the physical-chemical reference material The ielab reference materials are obtained through Proficiency Testing Schemes accredited by the Spanish National Accreditation Body (ENAC), in which a great number of national and international laboratories take part. Each of these laboratories that are participating in Proficiency Testing Schemes have different working methodologies, which gives very robust results. For the production of these materials, the standards established in the ISO GUIDE 34 and in the guide ILAC-G -12:2000, which indicate the general requirements for all the reference materials producers, are followed. The greatest part of the provided material is of natural origin and with analyte concentration levels similar to real samples that are performed in laboratories. Necessary homogeneity and stability tests are made to these materials to calculate the property value according to the ISO GUIDE 35. The certificate that accompanies the material fulfils the established requirements of the ISO GUIDE 31 and UNE 82131:2003 IN including the corresponding homogeneity and stability studies, the statistical study of results and the concentration and uncertainties of the provided materials. Advantages of the physical-chemical reference material Quality guarantee: For its production the standards established in the ISO GUIDE 34 are followed, statistical criteria defined in the ISO GUIDE 35 have been used to obtain the property value and the certificate fulfills the ISO GUIDE 31. Natural: The greatest part of the provided materials are of NATURAL ORIGIN, allowing to reproduce customary testing conditions. Stable: The stability of the samples is proven according to the established under the standard ISO 13528:2005, being analyzed by a method accredited under the standard EN ISO/IEC Homogeneous: Statistical studies are carried out of the variability, as it is established in the ISO GUIDE 35 and according to the IUPAC protocol, being analyzed by a method accredited under the standard EN ISO/IEC Flexible: Materials for various matrixes and with many different parameters are available. 5

6 WASTEWATERS (part nr. I061000) Coming from WWTP with urban or industrial origin. These reference materials are provided mostly in containers of 500 ml. DRINKING WATERS Type 1 (part nr. I061014) Type 2 (part nr. I061017) Type 3 (part nr. I061057) Drinking waters coming from potable water supply. These reference materials are provided customarily in containers of 500 ml or in vials of 10mL with a concentrated solution of organic compounds. SLUDGES (part nr. I061061) Sludges coming from WWTP with urban or industrial origin. These reference materials are provided in containers of 65 ml containing dry and sieved sludge. SAMPLE SUPPORTS (part nr. I061089) These reference materials are presented in transparent cases which contain the filters. Each supply of sample supports consists of three filter blanks and three spiked filters. SEA WATERS (part nr. I061103) Taken directly of the Mediterranean sea. These reference materials are provided in containers of 500 ml, to exception of the materials AM 5 and AM 6 that are packed in vials of 100 ml. SOILS (part nr. I061111) These reference materials are presented in bottles containing 65 ml of dried and sieved soil. ABSORPTION SOLUTION (part nr. I061074) These reference materials are provided in containers with 500 ml. A blank reference sample is always included with the spiked sample capture solution. PCBs EN DIELECTRIC OILS (part nr. I061099) These reference materials are provided in containers of 65 ml. The PCBs (Polychlorinated biphenyls) that are present are the ones that are included in the Directive 96/59/CE. 6 On the attached document (Appendix) you can find the current batches of each of the matrixes, including the analytes and its corresponding concentration and uncertainty.

7 Microbiological reference material ielab presents, within the framework of its line of products for microbiological diagnostics, a new range of reference materials, which have been specially designed to facilitate all stages of quality control performed in diagnostic laboratories. Properties of the microbiological reference material The ielab microbiological reference materials are quantified by certification tests performed by seven laboratories accredited by ENAC (Spanish National Accreditation Body) according to the norm EN ISO/IEC For the production of these materials, the established under the ISO GUIDE 34 and the guide ILAC-G -12:2000 are followed. These indicate the general requirements for all the producers of reference materials. All supplied material include a Quick User Guide and the corresponding Certificate of Analysis. Advantages of the microbiological reference material Simple: The preparation of the material is extremely simple, as the presentation is in tablet form (except for BACuanti-DNA) and is supplied in an easy-to-use sterile plastic device, or in individual vials, avoiding the need of multiple manipulations. Quick: It takes only 3 steps and 10 minutes to have ready bacterial suspensions of a known count. Easy storage: In a conventional freezer. Safe: There is no risk of contamination, as manipulation is reduced to the minimum. Traceable: The used strains are traceable to different Type Culture Collections and bearing less than two subcultures from the original strain. Known concentration: The existing concentration of the material is certified. Flexible: There are different formats for diverse microorganisms, and are available in two concentration ranges: low concentration and high concentration. Service: Apart from the species available from stock, there is the possibility of preparing reference material for other microorganisms at a concentration predefined by the client. Please contact our Sales Department for further information. 7

8 BAControl This is a quantitative material in which each tablet contains a specific number of viable, culturable cells, obtained by the manufacturer under the test conditions specified in the Certificate of Analysis. It is a material which is appropriate for carrying out routine quality control procedures, such as process controls, creation of control charts, or quality control of culture media. It has a shelf life of 6 months from the manufacturing date and is provided in boxes containing 10 vials with one tablet each or in a sterile dispenser of 5 tablets each. Those species labeled as PHARMA correspond to those requested by the norms of European Pharmacopoeia, supplement 6.5. Aspergillus brasiliensis (niger) A. brasiliensis ATCC A. brasiliensis ATCC <100 >100 I I A. brasiliensis ATCC A. brasiliensis ATCC <100 >100 I I Aspergillus brasiliensis (niger) - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. A. brasiliensis ATCC A. brasiliensis ATCC <100 >100 I I A. brasiliensis ATCC A. brasiliensis ATCC <100 >100 I I Bacillus subtilis B. subtilis ATCC 6633 <100 I B. subtilis ATCC 6633 >100 I B. subtilis ATCC 6633 B. subtilis ATCC 6633 <100 >100 I I * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml.

9 Bacillus subtilis - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. B. subtilis ATCC 6633 <100 I B. subtilis ATCC 6633 >100 I B. subtilis ATCC 6633 <100 I B. subtilis ATCC 6633 >100 I Candida albicans. C. albicans ATCC I C. albicans ATCC I C. albicans ATCC C. albicans ATCC I I Candida albicans - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No.. C. albicans ATCC I C. albicans ATCC I C. albicans ATCC C. albicans ATCC I I Citrobacter freundii. C. freundii NCTC 6272 C. freundii NCTC 6272 I I C.freundii NCTC 6272 C. freundii NCTC 6272 I I Recommended use, other than for the microorganism itself, for aerobic bacteria, coliform bacteria (total coliforms) and fecal coliforms. * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml. 9

10 Clostridium perfringens C. perfringens ATCC I C. perfringens ATCC I C. perfringens ATCC C. perfringens ATCC I I Recommended use, other than for the microorganism itself, for sulphite-reducing clostridia. Clostridium sporogenes C. sporogenes ATCC I C. sporogenes ATCC I C. sporogenes ATCC C. sporogenes ATCC I I Clostridium sporogenes - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. C. sporogenes ATCC C. sporogenes ATCC I I C. sporogenes ATCC C. sporogenes ATCC I I Enterococcus faecium E. faecium ATCC I E. faecium ATCC I E. faecium ATCC E. faecium ATCC I I Recommended use, other than for the microorganism itself, for aerobic bacteria, fecal enterococcus and streptococcus. 10 * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml.

11 Escherichia coli E. coli ATCC I E. coli ATCC I E. coli ATCC I E. coli ATCC I Recommended use, other than for the microorganism itself, for aerobic bacteria, coliform bacteria (total coliforms) and fecal coliforms. E. coli ATCC 8739 E. coli ATCC 8739 I I E. coli ATCC 8739 E. coli ATCC 8739 I I Recommended use, other than for the microorganism itself, for aerobic bacteria, coliform bacteria (total coliforms) and fecal coliforms. Escherichia coli - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. E. coli ATCC 8739 E. coli ATCC 8739 I I E. coli ATCC 8739 E. coli ATCC 8739 I I Legionella pneumophila L. pneumophila ATCC L. pneumophila ATCC I I L. pneumophila ATCC L. pneumophila ATCC I I * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml. 11

12 Legionella jordanis L. jordanis NCTC L. jordanis NCTC I I L. jordanis NCTC I L. jordanis NCTC I Listeria monocytogenes L. monocytogenes ATCC L. monocytogenes ATCC I I L. monocytogenes ATCC L. monocytogenes ATCC I I Pseudomonas aeruginosa P. aeruginosa ATCC P. aeruginosa ATCC I I P. aeruginosa ATCC P. aeruginosa ATCC I I P. aeruginosa ATCC 9027 P. aeruginosa ATCC 9027 I I P. aeruginosa ATCC 9027 P. aeruginosa ATCC 9027 I I * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml.

13 Pseudomonas aeruginosa - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. P. aeruginosa ATCC 9027 I P. aeruginosa ATCC 9027 I P. aeruginosa ATCC 9027 I P. aeruginosa ATCC 9027 I Salmonella sp. S. enterica subsp. enterica ATCC S. enterica subsp. enterica ATCC I I S. enterica subsp. enterica ATCC I S. enterica subsp. enterica ATCC I Salmonella enterica subsp. enterica serovar Abony S. Abony ATCC 6017 S. Abony ATCC 6017 I I S. Abony ATCC 6017 S. Abony ATCC 6017 I I Salmonella enterica subsp. enterica serovar Abony - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. S. Abony ATCC 6017 S. AbonyATCC 6017 I I S. Abony ATCC 6017 S. Abony ATCC 6017 I I * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml. 13

14 Salmonella enterica subsp.enterica serovar Typhimurium S. typhimurium ATCC I S. typhimurium ATCC I S. typhimurium ATCC I S. typhimurium ATCC I Salmonella enterica subsp.enterica serovar Typhimurium -línea PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. S.typhimurium ATCC S.typhimurium ATCC I I S.typhimurium ATCC I S.typhimurium ATCC I Staphylococcus aureus S. aureus ATCC S. aureus ATCC I I S. aureus ATCC I S. aureus ATCC I S.aureus ATCC 6538 I S.aureus ATCC 6538 I S.aureus ATCC 6538 I S.aureus ATCC 6538 I Staphylococcus aureus - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. S.aureus ATCC 6538 I S.aureus ATCC 6538 I S.aureus ATCC 6538 S.aureus ATCC 6538 I I * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml.

15 Helminths This quantitative material is provided in vials containing 150 eggs/vial. It has a shelf life of 12 months from the manufacturing date. traceable strain Part No Fasciola hepatica I Fasciola gigantica Ascaris lumbricoides Taenia solium Trichuris sp. Schistosoma sp. Diphyllobothrium sp. I I I I I I Supplementary material BACwater: box with 10 vials containing sterile water BACglass: box with 10 sterile vials Part No I I * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml. 15

16 BACuanti This is a quantitative material in which each tablet contains a specific number of viable and culturable cells, obtained from the certification studies performed by seven accredited laboratories according to EN ISO/IEC The material is appropriate for the validation of methods, and quantitative quality controls. It has a shelf life of 6 months from the date of manufacturing and is provided in a sterile dispenser of 5 tablets. Those species labeled as PHARMA correspond to those requested by the norms of European Pharmacopoeia, supplement 6.5. Aspergillus brasiliensis (niger) A. brasiliensis ATCC <100 I A. brasiliensis ATCC >100 I Aspergillus brasiliensis (niger) - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. A. brasiliensis ATCC A. brasiliensis ATCC <100 >100 I I Bacillus subtilis B. subtilis ATCC 6633 B. subtilis ATCC 6633 <100 >100 I I Bacillus subtilis - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. B. subtilis ATCC 6633 B. subtilis ATCC 6633 <100 >100 I I Candida albicans C. albicans ATCC C. albicans ATCC <100 >100 I I Candida albicans - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. C. albicans ATCC <100 I C. albicans ATCC >100 I * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml.

17 Citrobacter freundii C. freundii NCTC 6272 C. freundii NCTC 6272 I I Recommended use, other than for the microorganism itself, for aerobic bacteria, coliform bacteria (total coliforms) and fecal coliforms. Clostridium perfringens C. perfringens ATCC C. perfringens ATCC I I Recommended use, other than for the microorganism itself, for sulphite-reducing clostridia. Clostridium sporogenes C. sporogenes ATCC I C. sporogenes ATCC I Clostridium sporogenes - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. C. sporogenes ATCC I C. sporogenes ATCC I Enterococcus faecium E. faecium ATCC I E. faecium ATCC I Recommended use, other than for the microorganism itself, for aerobic bacteria, fecal enterococcus and streptococcus. Escherichia coli E. coli ATCC E. coli ATCC I I Recommended use, other than for the microorganism itself, for aerobic bacteria, coliform bacteria (total coliforms) and fecal coliforms. E. coli ATCC E. coli ATCC I I Recommended use, other than for the microorganism itself, for aerobic bacteria, coliform bacteria (total coliforms) and fecal coliforms. * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml. 17

18 Escherichia coli - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. E. coli ATCC 8739 E. coli ATCC 8739 I I Recommended use, other than for the microorganism itself, for aerobic bacteria, coliform bacteria (total coliforms) and fecal coliforms. Legionella pneumophila L. pneumophila ATCC I L. pneumophila ATCC I Legionella jordanis L. jordanis NCTC I L. jordanis NCTC I Listeria monocytogenes L. monocytogenes ATCC L. monocytogenes ATCC I I Pseudomonas aeruginosa P. aeruginosa ATCC I P. aeruginosa ATCC I P. aeruginosa ATCC 9027 P. aeruginosa ATCC 9027 I I Pseudomonas aeruginosa - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. P. aeruginosa ATCC 9027 P. aeruginosa ATCC 9027 I I * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml.

19 Salmonella sp. S. enterica subsp. enterica ATCC I S. enterica subsp. enterica ATCC I Salmonella enterica subsp. enterica serovar Abony S. Abony ATCC 6017 I S. Abony ATCC 6017 I Salmonella enterica subsp. enterica serovar Abony-PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. S. Abony ATCC 6017 I S. Abony ATCC 6017 I Salmonella enterica subsp. enterica serovar Typhimurium S.typhimurium ATCC S.typhimurium ATCC I I Salmonella enterica subsp. enterica serovar Typhimurium -PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. S. typhimurium ATCC I S. typhimurium ATCC I Staphylococcus aureus S. aureus ATCC S. aureus ATCC I I S. aureus ATCC 6538 S. aureus ATCC 6538 I I Staphylococcus aureus - PHARMA traceable strain concentration (cfu/0.1 ml)* Part No. S. aureus ATCC 6538 S. aureus ATCC 6538 I I * According to the User's Guide of this product, concentration is calculated after dissolving the tablet in 20 ml. 19

20 BACuali This is a qualitative material in which the identity of the bacterial strain is certified. Appropriate for validation of qualitative methods and for the accomplishment of quality controls where quantification is not required. It has a shelf life of 9 months from the date of manufacturing and is provided in a sterile dispenser of 5 tablets. traceable strain Aspergillus brasiliensis (niger) ATCC Aspergillus brasiliensis (niger) ATCC PHARMA Bacillus subtilis ATCC 6633 Bacillus subtilis ATCC PHARMA Candida albicans ATCC Candida albicans ATCC PHARMA part No. I I I I I I Citrobacter freundii NCTC 6272 I Recommended use, other than for the microorganism itself, for aerobic bacteria, coliform bacteria (total coliforms) and fecal coliforms. Clostridium perfringens ATCC Recommended use, other than for the microorganism itself, for sulphite-reducing clostridia. Clostridium sporogenes ATCC PHARMA I I Enterococcus faecium ATCC I Recommended use, other than for the microorganism itself, for aerobic bacteria, fecal enterococcus and streptococcus. Escherichia coli ATCC I Recommended use, other than for the microorganism itself, for aerobic bacteria, coliform bacteria (total coliforms) and fecal coliforms. Escherichia coli ATCC PHARMA I

21 BACuali (PHARMA) traceable strain Legionella pneumophila ATCC Legionella jordanis NCTC Listeria monocytogenes ATCC Pseudomonas aeruginosa ATCC Pseudomonas aeruginosa - ATCC PHARMA Salmonella enterica subsp.enterica ATCC Salmonella enterica subsp.enterica part No. I I I I I I I serovar Abony ATCC PHARMA Salmonella enterica subsp.enterica I serovar Thyphimurium ATCC PHARMA Staphylococcus aureus ATCC Staphylococcus aureus - ATCC PHARMA I I

22 Materials for molecular biology (PCR and DNA) BACuanti-PCR This is a quantitative material specially designed for PCR, in which each tablet contains a specific number of inactivated cells, being certified the number of cells per tablet. This allows a control over the whole analytical process, from the initial sample to the final analysis. It has a shelf life of 12 months from the date of manufacturing and is provided in a sterile dispenser of 5 tablets. The currently available microorganisms are: Legionella pneumophila traceable strain concentration Part No. L. pneumophila ATCC L. pneumophila ATCC low (>4 and <6 log) high (>6 and <8 log) I I

23 BACuanti-DNA This is a quantitative material which is presented in freeze-dried format inside a vial, being certified the quantity in µg/vial. Designed for validation and quality control of the PCR amplification step. It has a shelf life of 9 months from the date of manufacturing and is provided in individual vials, including a vial with PCR-grade distilled water. The currently available microorganisms are: Legionella pneumophila traceable strain Part No. L. pneumophila ATCC I Escherichia coli traceable strain Part No. Escherichia coli ATCC I

24 PROFICIENCY TESTING SCHEMES ielab is a company engaged to provide services and products for the application of quality in testing laboratories, where we think that quality is synonym to calmness and reliance. We offer the organization of Proficiency Testing Schemes on international basis, molecular diagnostic kits and microbiological and physicalchemical reference materials. We invite you to visit our web page: where you can find our latest and detailed information and the availability of our products and services. ielab is accredited by ENAC (Spanish National Accreditation Body) to undertake Proficiency Testing Schemes on the basis of the standard ISO/IEC 17043:2010, GUIDE ILAC-13 and CGA-ENAC-PPI (scope nr. 2/PPI007), with many years of experience and more than 1100 participants of 34 countries in the last year. The usage of suppliers of accredited Proficiency Testing Schemes provides important advantages to laboratories and by this way to be able to show their knowledge and their quality level to both, current or future customers and to the accreditation or certification entities. PROPERTIES OF THE ielab PROFICIENCY TESTS 1. Participation code Ensures the confidentiality of the participant laboratory. 2. Samples Ielab provides real life samples and these are according to the IUPAC criteria to assure its homogeneity and stability. 3. Sample distribution and online report sheet Sending of the samples by urgent courier and online management of the analytical results, for a better agility and segurity in the process. 4. Statistical treatment According to the IUPAC criteria and to ISO 13528:2005. For each parameter its consensus value, its standard deviation and its uncertainty are calculated. The laboratories are assessed by the Z-SCORE criteria. 5. Reports Sending of the reports of each round by , containing the results, the corresponding statistical studies, the used methods, tables and control charts, etc. 6. Final meeting and Certificate Possibility to raise questions or debate on the development of the PTS. Certificates of Participation will be available to download from the ielab webpage during the year.

25 2011 ielab PROFICIENCY TESTS 9 matrixes 23 PTs 160 parameters DRINKING WATERS Drinking waters A Drinking waters B Drinking waters C Microbiology in drinking waters CONTINENTAL WATERS Directive 2000/60/EC (list of priority substances) Raw waters DRO+GRO+Hydrocarbons Microbiology in continental waters WASTEWATERS Wastewaters Reclaimed waters (physical - chemical and microbiological parameters) Solids in wastewaters Microbiology in wastewaters SEA WATERS Sea waters (physical - chemical and microbiological parameters) SOLIDS Organic compounds in solids Sludges Residues Soils SAMPLE SUPPORTS Atmospheric pollution LEGIONELLA - culture LEGIONELLA -PCR BIOTA OTHERS PCBs in dielectric oils ANALYSIS "IN SITU" Analysis "in situ" OTHER ielab PRODUCTS OF YOUR INTEREST Microbiological reference material (document ) Physical-chemical reference material (document ) Molecular diagnostic kits (document ) C/ Dracma, 16 Pol. Ind. Las Atalayas Alicante (Spain) T F comercial@ielab.es

26 A P P E N D I X T O T H E PHYSICAL-CHEMICAL REFERENCE MATERIAL CATALOGUE (edition: February 2011)

27 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 INDEX page WASTEWATER 4 DRINKING WATER 5 SLUDGES 9 SAMPLE SUPPORTS 11 SEA WATER 12 SOILS 13 ABSORTION SOLUTIONS 14 PCBs 15 page 3 of 16

28 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 WASTEWATERS part nr. I Part nr. Analyte Property value Uncertainty Units RES 1.2 Chromium 0,5 0,007 mg/l Iron 1050,0 0,013 mg/l RES 2.1 Kjeldahl Nitrogen 2,1 0,071 mg/l RES 2.2 Cadmium 0,1 0,0022 mg/l RES 4.2 Cadmium 1,0 0,015 mg/l Copper 3,0 0,038 mg/l RES 5.1 Orthophosphates 4,2 0,039 mg/l RES 5.2 Lead 0,4 0,006 mg/l Chromium 1,5 0,021 mg/l RES 6.2 Nickel 0,9 0,014 mg/l Iron 2740,0 0,031 mg/l RES 7.1 Total Phosphorus 14,0 0,083 mg/l RES 8.1 Kjeldahl Nitrogen 31,4 0,234 mg/l Nitrates 21,1 0,215 mg/l RES 9.1 Orthophosphates 16,7 0,161 mg/l Total Nitrogen 58,9 0,445 mg/l RES 10.2 Copper 1,0 0,006 mg/l Chromium 4,6 0,055 mg/l RES 11.1 Total Phosphorus 3,6 0,026 mg/l Orthophosphates 10,0 0,071 mg/l TOC 16,8 0,505 mg/l RES 11.2 Cadmium 0,9 0,013 mg/l Nickel 1,5 0,021 mg/l RES 12.1 Total Nitrogen 105,1 0,744 mg/l Kjeldahl Nitrogen 103,6 0,428 mg/l RES 12.2 Boron 1,4 0,014 mg/l Lead 1,0 0,015 mg/l Iron 1,1 0,013 mg/l RES 13.1 Ammonium 47,7 0,349 mg/l Nitrates 19,0 0,272 mg/l RES 13.2 Copper 1,7 0,015 mg/l Chromium 2,3 0,031 mg/l RES 14.1 Total Phosphorus 4,8 0,034 mg/l Orthophosphates 12,0 0,135 mg/l TOC 38,8 1,063 mg/l RES 14.2 Cadmium 1,5 0,009 mg/l Nickel 0,9 0,007 mg/l RES 15.1 Total Nitrogen 75,1 0,583 mg/l Kjeldahl Nitrogen 74,5 0,387 mg/l RES 15.2 Boron 0,9 0,011 mg/l Lead 1,1 0,012 mg/l Iron 2,1 0,002 mg/l page 4 of 16

29 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 DRINKING WATERS typ pe 1 part nr. I Part nr. Analyte Property value Uncertainty Units TYPE 1 POT 1.1 Aluminium 147,2 2,13 µg/l Cadmium 12,0 0,12 µg/l Manganese 47,5 0,5 µg/l Lead 20,0 0,23 µg/l POT 1.2 Nitrates 3,4 0,028 mg/l Boron 0,5 0,005 mg/l POT 2.1 Arsenic 12,8 0,13 µg/l Iron 101,2 1 µg/l Selenium 9,8 0,27 µg/l POT 2.2 Magnesium 22,2 0,19 mg/l Sodium 87,9 0,55 mg/l Chlorides 143,3 0,44 mg/l POT 2.3 ph 6,5 0,003 U ph POT 3.1 Copper 1,0 0,005 mg/l Nickel 15,0 0,154 µg/l Chromium 39,9 0,392 µg/l Antimony 10,0 0,298 µg/l POT 3.2 Potassium 6,9 0,05 mg/l POT 4.1 Arsenic 15,0 0,176 µg/l Copper 0,8 0,007 mg/l Iron 0,3 0,003 mg/l POT 5.1 Aluminium 502,0 6 µg/l Chromium 59,1 0,641 µg/l Manganese 78,5 0,793 µg/l Nickel 25,3 0,264 µg/l POT 7.1 Aluminium 340, µg/l Iron 494, µg/l Antimony 15,5 0,432 µg/l Cadmium 6,2 0,067 µg/l POT 8.1 Arsenic 10,0 0,114 µg/l Copper 671, µg/l Chromium 35,0 0,346 µg/l Manganese 53,3 0,493 µg/l POT 8.2 Magnesium 29,4 0,097 mg/l Boron 0,6 0,006 mg/l Nitrates 1,5 0,022 mg/l POT 9.1 Mercury 0,9 0,0117 µg/l Lead 29,5 0,3127 µg/l Nickel 19,0 0,2042 µg/l Zinc 58,6 0,7809 µg/l Selenium 10,0 0,2729 µg/l POT 9.2 Sulfates 98,3 0,423 mg/l Potassium 4,5 0,036 mg/l POT 9.3 Colour 8,0 0,094 mg/l POT 10.1 Aluminium 485, µg/l Antimony 12,0 0,338 µg/l Manganese 59,0 0,545 µg/l Cadmium 8,6 0,086 µg/l POT 10.2 Conductivity 764,0 5,68 µs/cm Magnesium 19,3 0,101 mg/l Sodium 86,8 0,456 mg/l POT 11.1 Iron 229,0 2,09 µg/l Arsenic 20,9 0,229 µg/l Mercury 1,7 0,032 µg/l Selenium 16,0 0,331 µg/l POT 11.2 ph 6,3 0,003 U ph POT 11.4 Chlorides 146,8 0,377 mg/l Potassium 8,6 0,054 mg/l POT 12.1 Lead 44,6 0,462 µg/l Copper 1,6 0,007 mg/l Chromium 64,9 0,656 µg/l Nickel 28,9 0,285 µg/l POT 12.2 Fluorine 0,2 0,003 mg/l Sulfates 59,2 0,34 mg/l Calcium 32,7 0,12 mg/l POT 13.1 Aluminium 394,0 4,903 µg/l Antimony 21,3 0,57 µg/l Manganese 97,0 0,766 µg/l Cadmium 8,2 0,078 µg/l POT 14.1 Iron 386,9 3,04 µg/l Arsenic 18,1 0,241 µg/l Mercury 0,9 0,014 µg/l Selenium 10,6 0,209 µg/l POT 14.2 Ph 5,7 0,004 UpH POT 14.3 Color 7,7 0,136 mg/l POT 14.4 Chlorides 96,3 0,259 mg/l Potassium 2,8 0,025 mg/l page 5 of 16

30 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 DRINKING WATERS type 1 part nr. I DRINKING WATERS type 2 part nr. I Part nr. Analyte Property value Uncertainty Units TYPE 1 (continues) POT 15.1 Lead 31,0 0,357 µg/l Copper 746,0 3,939 µg/l Nickel 23,9 0,228 µg/l Chromium 55,9 0,546 µg/l POT 15.2 Turbidity 1,7 0,017 UNF Fluorides 0,5 0,005 mg/l Sulfates 100,4 0,427 mg/l Calcium 51,3 0,168 mg/l Part nr. Analyte Property value Uncertainty Units TYPE 2 POT 4.3 Cloroform 14,9 0,5290 µg/l Bromoform 4,2 0,1580 µg/l 1,1,1-Trichloroethane 8,5 0,3810 µg/l o-xylene 7,5 0,2940 µg/l POT 4.4 Benzo-b-fluoranthene 0,0 0,0014 µg/l Benzo-(g,h,i)-perylene 0,1 0,0029 µg/l Aldrin 0,0 0,0019 µg/l Dieldrin 0,1 0,0023 µg/l Propazine 0,2 0,0087 µg/l POT 5.4 Tetrachloroethene 3,0 0,1050 µg/l Trichloroethene 8,0 0,2720 µg/l 1,2-Dichloroethane 2,0 0,0750 µg/l Toluene 12,9 0,5460 µg/l POT 5.5 Benzo-a-pyrene 0,0 0,0007 µg/l Indeno-(1,2,3-c,d)-pyrene 0,1 0,0032 µg/l Heptachlor 0,2 0,0067 µg/l Alfa-endosulfan 0,1 0,0034 µg/l Beta-endosulfan 0,1 0,0043 µg/l POT 7.5 Benzo-a-pyrene 0,020 0,0007 µg/l 4,4 -DDE 0,072 0,0030 µg/l Atrazine 0,162 0,0070 µg/l Simazine 0,188 0,0079 µg/l Heptachlor 0,040 0,0016 µg/l POT 8.4 Dibromochloromethane 33, ,0000 µg/l Dichlorobromomethane 18,000 0,6490 µg/l 1,2-Dichloroethane 3,500 0,1360 µg/l 1,1,1-Trichloroethane 7,400 0,3080 µg/l POT 8.5 Aldrin 0,038 0,0015 µg/l Dieldrin 0,072 0,0027 µg/l Beta-endosulfan 0,128 0,0050 µg/l Benzo-b-fluoranthene 0, , µg/l Benzo-k-fluoranthene 0,054 0,0020 µg/l Ametryn 0,097 0,0051 µg/l POT 9.4 Benzene 3,030 0,1160 µg/l Toluene 20,000 0,8220 µg/l o-xylene 10,000 0,4110 µg/l Ethylbenzene 5,030 0,2100 µg/l POT 9.5 Indeno-(1,2,3-c,d)-pyrene 0,101 0,0039 µg/l Benzo-(g,h,i)-perylene 0,090 0,0035 µg/l Fluoranthene 0,085 0,0038 µg/l Propazine 0,150 0,0073 µg/l Terbutylazine 0,177 0,0075 µg/l POT ,2-Dichloroethane 4,950 0,1830 µg/l 1,1,1-Trichloroethane 10,000 0,4000 µg/l Dibromochloromethane 25,000 0,8270 µg/l Dichlorobromomethane 14,900 0,4790 µg/l POT 10.4 Benzo-a-pyrene 0,051 0,0020 µg/l Benzo-b-fluoranthene 0,070 0,0030 µg/l Aldrin 0,100 0,0040 µg/l Dieldrin 0,103 0,0040 µg/l Atrazine 0,202 0,0080 µg/l Ametryn 0,093 0,0050 µg/l POT 11.5 Bromoform 9,160 0,2750 µg/l Cloroform 25,100 0,8440 µg/l Benzene 5,020 0,1920 µg/l Toluene 15,000 0,6430 µg/l POT 11.6 Benzo-g,h,i-perilene 0,150 0,0056 µg/l Alfa-endosulfan 0,200 0,0088 µg/l Heptachlor 0,100 0,0040 µg/l Indeno-1,2,3-c,d-pirene 0,148 0,0058 µg/l Propazine 0,109 0,0050 µg/l POT 12.3 Terbutilazine 0,120 0,0054 µg/l Trichloroethene 14,560 0,4860 µg/l Tetrachloroethene 11,000 0,3710 µg/l Ethylbenzene 13,320 0,5400 µg/l o-xylene 30,500 1,2700 µg/l page 6 of 16

31 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 Part nr. Analyte Property value Uncertainty Units TYPE 2 (continues) POT 12.4 Benzo-k-fluoranthene 0,102 0,0040 µg/l Beta-endosulfan 0,191 0,0080 µg/l 4,4 -DDE 0,090 0,0040 µg/l Fluoranthene 0,080 0,0030 µg/l Simazine 0,246 0,0110 µg/l Heptachlor epoxide 0,090 0,0040 µg/l POT 13.2 Conductivity 758,000 1,1660 mg/l Bicarbonates 140,330 0,6060 mg/l Magnesium 26,320 0,1450 mg/l Sodium 69,400 0,4000 mg/l POT ,2-Dichloroethane 7,430 0,2830 µg/l 1,1,1-Trichloroethane 5,740 0,2580 µg/l Dibromochloromethane 11,790 0,2500 µg/l Dichlorobromomethane 19,400 0,4380 µg/l POT 13.4 Benzo-a-pyrene 0,085 0,0026 µg/l Benzo-b-fluoranthene 0,095 0,0024 µg/l Aldrin 0,130 0,0046 µg/l Dieldrin 0,136 0,0042 µg/l Atrazine 0,229 0,0073 µg/l Ametryn 0,069 0,0037 µg/l POT 14.5 Bromoform 13,750 0,2470 µg/l Chloroform 10,870 0,2820 µg/l Benzene 7,470 0,2590 µg/l Toluene 21,220 0,5650 µg/l POT 14.6 Benzo-g,h,i-perilene 0,074 0,0027 µg/l Alfa-endosulfan 0,091 0,0043 µg/l Heptachlor 0,090 0,0028 µg/l Indeno-1,2,3-c,d-pirene 0,108 0,0040 µg/l Propazine 0,115 0,0044 µg/l Terbutilazine 0,222 0,0085 µg/l POT 15.3 Trichloroethene 24,360 0,7430 µg/l Tetrachloroethene 17,850 0,5480 µg/l Ethylbenzene 18,790 0,6750 µg/l O-xilene 36,690 1,2610 µg/l POT 15.4 Benzo-k-fluoranthene 0,193 0,0088 µg/l Beta endosulfan 0,017 0,0009 µg/l 4,4 -dde 0,019 0,0010 µg/l Fluoranthene 0,102 0,0041 µg/l Simazine 0,264 0,0082 µg/l Heptachlor epoxide 0,012 0,0005 µg/l page 7 of 16

32 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 DRINKING WATERS type 3 part nr. I Part nr. Analyte Property value Uncertainty Units TYPE 3 POTC 1 Calcium 50,2 0,53 mg/l Silica 6,8 0,11 mg/l POTC 2 Total Phosphorus 4,4 0,08 mg/l Kjeldahl Nitrogen 1,6 0,09 mg/l POTC 3 Anionic Surfactants 0,6 0,028 mg/l POTC 4 Cobalt 29,9 1,76 µg/l Vanadium 40,0 2,44 µg/l POTC 5 Magnesium 19,6 0,235 mg/l Hardness 18,3 0,152 mg/l Dry Matter 528,0 11,186 mg/l POTC 6 Phenols 203,0 12,422 µg/l POTC 9 Calcium 42,6 0,500 mg/l Bicarbonates 95,0 1,010 mg/l Hardness 16,8 0,179 ºF Dry Matter 453,0 11,350 mg/l POTC 10 Barium 178,1 10,245 µg/l Berilium 99,0 6,290 µg/l Vanadium 54,0 3,300 µg/l POTC 11 Phenols 352,0 19,053 µg/l POTC 12 Magnesium 15,4 0,118 mg/l Silica 11,2 0,209 mg/l POTC 13 Anionic surfactants 0,5 0,024 mg/l Kjeldahl Nitrogen 2,6 0,119 mg/l Total Phosphorus 6,4 0,066 mg/l POTC 14 Calcium 42,6 0,514 mg/l Bicarbonates 100,9 0,969 mg/l Hardness 16,6 0,136 ºF Dry Matter 375,9 6,182 mg/l POTC 15 Barium 100,6 0,99 µg/l Berilium 61,2 1,712 µg/l Vanadium 80,3 1,55 µg/l POTC 16 Phenols 348,6 37,267 µg/l POTC 17 Magnesium 14,6 0,149 mg/l Silica 2,3 0,093 mg/l POTC 18 Anionic Surfactants 591,0 16,231 µg/l Kjeldahl Nitrogen 3,7 0,174 mg/l Total Phosphorus 4,5 0,091 mg/l page 8 of 16

33 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 SLUDGES rt nr. I par Part nr. Analyte Property value Uncertainty Units LD-0301 Cadmium 0,8 0,07 mg/kg Copper 549,0 6,38 mg/kg Chromium 57,9 2,11 mg/kg Mercury 1,0 0,05 mg/kg Nickel 20,4 0,58 mg/kg Lead 60,6 1,36 mg/kg Zinc 594,0 8,03 mg/kg Arsenic 5,7 0,55 mg/kg ph 5,8 0,01 U ph Conductivity 5669,0 142,51 µs/cm Organic Matter 63,5 1,02 % LD-0302 Cadmium 20,2 0,25 mg/kg Copper 324,0 3,05 mg/kg Chromium 34,1 0,83 mg/kg Mercury 1,4 0,05 mg/kg Nickel 19,6 0,37 mg/kg Lead 66,1 1,22 mg/kg Zinc 661,0 7 mg/kg Arsenic 50,2 0,58 mg/kg Kjeldahl Nitrogen 3,0 0,02 % N ph 5,5 0,01 U ph LD-0305 Cadmium 8,4 0,144 mg/kg Copper 344,0 3,54 mg/kg Chromium 293,0 4,22 mg/kg Mercury 0,6 0,05 mg/kg Nickel 125,0 1,66 mg/kg Lead 176,0 2,5 mg/kg Zinc 971,0 13,08 mg/kg Iron 48063,0 528,75 mg/kg Total Phosphorus 6,1 0,101 % P2O5 Kjeldahl Nitrogen 3,9 0,029 % N LD-0307 Cadmium 1,7 0,092 mg/kg Copper 1281,0 15,68 mg/kg Chromium 457,0 7,86 mg/kg Mercury 0,6 0,071 mg/kg Nickel 631,0 9,67 mg/kg Lead 41,7 1,39 mg/kg Zinc 2592,0 43,47 mg/kg Manganese 104,0 2,48 mg/kg Selenium 60,0 2,59 mg/kg LD-0310 Cadmium 1,8 0,063 mg/kg Copper 1748,0 14,56 mg/kg Chromium 550,00 6,53 mg/kg Mercury 0,6 0,025 mg/kg Nickel 914,0 13,62 mg/kg Lead 49,0 1,02 mg/kg Zinc 5788,0 65,18 mg/kg Conductivity 6195,0 136,558 µs/cm Magnesium 1,2 0,01 % MgO Iron 3,8 0,057 % Fe2O3 LD 2 Cadmium 7,1 0,096 mg/kg Copper 153,0 1,87 mg/kg Chromium 44,3 1,3 mg/kg Mercury 2,6 0,054 mg/kg Nickel 21,4 0,58 mg/kg Lead 128,0 1,79 mg/kg Zinc 883,0 12,27 mg/kg Iron 31868,0 431,61 mg/kg Total Phosphorus 9,1 0,102 mg/kg Organic Matter 57,2 0,288 % LD 3 Cadmium 14,8 0,192 mg/kg Copper 271,0 2,36 mg/kg Chromium 42,3 0,75 mg/kg Mercury 0,9 0,045 mg/kg Nickel 26,8 0,52 mg/kg Lead 78,1 1,18 mg/kg Zinc 689,0 7,6 mg/kg Conductivity 4622,0 85,67 µs/cm Magnesium 0,7 0,013 % MgO Kjeldahl Nitrogen 4,3 0,016 % N LD 4 Cadmium 61,5 0,829 mg/kg Copper 205, mg/kg Chromium 59, mg/kg Mercury 1,9 0,057 mg/kg Nickel 26,2 0,651 mg/kg Lead 177, mg/kg Zinc 4896, mg/kg Conductivity 5807, µs/cm Aluminium 14532, mg/kg Kjeldahl Nitrogen 4,5 0,034 % N page 9 of 16

34 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 Part nr. Analyte Property value Uncertainty Units SLUDGES part nr. I LD 7 Cadmium 144, mg/kg Copper 220,0 2,61 mg/kg Chromium 75,7 2,55 mg/kg Mercury 1,9 0,063 mg/kg Nickel 39,9 1,35 mg/kg Lead 254,0 3,27 mg/kg Zinc 2921,0 37,42 mg/kg Arsenic 27,2 0,57 mg/kg Aluminium 15634,0 643,532 mg/kg ph 5,9 0,013 U ph LD 8 Cadmium 3,2 0,05 mg/kg Copper 341,0 3,37 mg/kg Chromium 78,8 2,11 mg/kg Mercury 1,9 0,04 mg/kg Nickel 51,6 1,52 mg/kg Lead 323,0 4,22 mg/kg Zinc 1249,0 15,51 mg/kg Iron 15068,0 224 mg/kg Total Phosphorus 4,6 0,07 %P2O5 Conductivity 9709,0 214,24 µs/cm LD 10 Cadmium 3,0 0,07 mg/kg Copper 338,0 3,24 mg/kg Chromium 80,3 2,8 mg/kg Mercury 1,9 0,06 mg/kg Nickel 56,0 1,75 mg/kg Lead 323,0 4,12 mg/kg Zinc 1250,0 14,7 mg/kg ph 6,5 0,02 U ph Iron 14676,0 263,14 mg/kg Kjeldahl Nitrogen 4,6 0,03 % N LD 11 Cadmium 80,9 1,24 mg/kg Copper 269,0 3,97 mg/kg Chromium 73,6 1,93 mg/kg Mercury 1,7 0,05 mg/kg Nickel 28,5 0,849 mg/kg Lead 187,0 2,29 mg/kg Zinc 3924,0 70,39 mg/kg Conductivity 5455,0 96,61 µs/cm Aluminium 15915,0 435,75 mg/kg Total Phosphorus 6,4 0,09 %P2O5 LD 12 Cadmium 57,0 1,07 mg/kg Copper 230,0 2,5 mg/kg Chromium 224,0 3,11 mg/kg Mercury 0,8 0,036 mg/kg Nickel 63,5 1 mg/kg Lead 92,9 1,5 mg/kg Zinc 2163,0 27,86 mg/kg Organic Matter 60,7 0,122 % Arsenic 52,1 1,57 mg/kg Manganese 145,0 2,09 mg/kg LD 14 Cadmium 69,1 0,96 mg/kg Copper 532,4 7,07 mg/kg Chromium 79,0 2,15 mg/kg Mercury 15,1 0,37 mg/kg Nickel 40,0 0,82 mg/kg Lead 118,6 2,03 mg/kg Zinc 1699,2 22,38 mg/kg Conductivity 5574,0 121,16 µs/cm Aluminium 17045,7 606,14 mg/kg Fósforo total 6,1 0,10 %P2O5 LD 15 Arsénico 32,7 0,76 mg/kg Cadmio 66,9 1,05 mg/kg Cobre 386,6 4,71 mg/kg Cromo 43,0 0,81 mg/kg Manganeso 89,4 1,67 mg/kg Materia orgánica 66,3 0,20 % Mercurio 0,9 0,03 mg/kg Niquel 25,4 0,59 mg/kg Plomo 58,1 1,04 mg/kg Zinc 843,5 12,76 mg/kg page 10 of 16

35 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 SAMPLE SUPPORTS part nr. I Part nr. Analyte Property value Uncertainty Units AT 0403 Arsenic 10 0,482 µg/filter Manganese 12 0,554 µg/filter Nickel 20,6 0,965 µg/filter Selenium 5,1 0,282 µg/filter Vanadium 6,8 0,348 µg/filter AT 0405 Arsenic 9,14 0,584 µg/filter Manganese 5,73 0,331 µg/filter Nickel 7,8 0,435 µg/filter Selenium 13,7 0,859 µg/filter Vanadium 30,8 1,585 µg/filter FILTROS 1 Antimony 15,84 0,755 µg/filter Cobalt 8,06 0,431 µg/filter Chromium 12,19 0,686 µg/filter Lead 45, µg/filter Zinc 25, µg/filter FILTROS 2 Arsenic 7,07 0,294 µg/filter Copper 30, µg/filter Tin 15,06 0,839 µg/filter Manganese 5,01 0,24 µg/filter Selenium 10,01 0,461 µg/filter FILTROS 3 Cadmium 13,4 0,571 µg/filter Nickel 11,3 0,503 µg/filter Thallium 14,9 0,746 µg/filter Vanadium 8,03 0,438 µg/filter FILTROS 4 Antimony 39, µg/filter Cadmium 18,6 0,812 µg/filter Nickel 33, µg/filter Zinc 15,2 0,667 µg/filter Thallium 20, µg/filter FILTROS 5 Cobalt 16,4 0,747 µg/filter Chromium 21,4 0,945 µg/filter Tin 29, µg/filter Lead 15 0,651 µg/filter Selenium 26, µg/filter FILTROS 6 Arsenic 8,5 0,390 µg/filter Copper 10,1 0,473 µg/filter Vanadium 12,7 0,626 µg/filter Molybnenum 15,1 0,815 µg/filter FILTROS 9 Tin 20 0,947 µg/filter Vanadium 10 0,452 µg/filter Thallium 15 0,701 µg/filter Selenium 26, µg/filter FILTROS 11 Antimony 19,4 0,93 µg/filter Cadmium 13,8 0,56 µg/filter Chromium 8 0,341 µg/filter Tin 12 0,575 µg/filter Thallium 8,9 0,409 µg/filter FILTROS 12 Copper 24,1 1,08 µg/filter Lead 12,3 0,537 µg/filter Selenium 16,9 0,835 µg/filter Zinc 33,9 1,51 µg/filter FILTROS 13 Arsenic 8,54 0,1119 µg/filter Cobalt 7,49 0,0788 µg/filter Manganese 19,02 0,1799 µg/filter Nickel 16,56 0,1614 µg/filter Vanadium 11,49 0,1276 µg/filter page 11 of 16

36 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 SEA WATERS part nr. I Part nr. Analyte Property value Uncertainty Units AM 1 Orthophosphates 0,58 0,023 mg/l Ammonium 1 0,037 mg/l AM 2 Salinity 38,3 0,367 %o AM 4 Kjeldahl Nitrogen 3,05 0,155 mg/l Nitrates 4,4 0,145 mg/l AM 6 ph 8,38 0,008 U ph AM 7 Ammonium 0,78 0,028 mg/l Nitrates 2,37 0,086 mg/l AM 9 Orthophosphates 1,01 0,032 mg/l Kjeldahl Nitrogen 6,3 0,193 mg/l AM 10 Salinity 38,2 0,240 %o AM 11 Antimony 19,9 1,388 µg/l Lead 14,8 0,983 µg/l Mercury 1,99 0,107 µg/l AM 12 Ammonium 2,1 0,074 mg/l Nitrates 3,85 0,176 mg/l AM 13 Turbidity 1,25 0,054 UNF AM 14 ph 7,99 0,012 U ph AM 15 Cadmium 14,52 0,752 µg/l Arsenic 10,04 0,521 µg/l AM 16 Orthophosphates 1,99 0,028 mg/l Kjeldahl Nitrogen 3,28 0,257 mg/l AM 17 Salinity 37,93 0,253 AM 18 Antimony 13,04 0,445 µg/l Lead 18,05 0,779 µg/l Mercury 0,86 0,041 µg/l page 12 of 16

37 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 SOILS par nr. I Part nr. Analyte Property value Uncertainty Units SL 1 Copper 27,1 1,18 mg/kg Nickel 8,4 0,38 mg/kg Arsenic 46,3 1,41 mg/kg Selenium 52,6 2,29 mg/kg Sodium 0,056 0,01 % Na2O Magnesium 1,5 0,05 % MgO Potassium 0,42 0,04 % K2O ph 8,3 0,03 U ph SL 2 Chromium 21, mg/kg Lead 39, mg/kg Iron 1,07 0,044 % Fe2O3 Phosphorus 0,345 0,005 %P2O5 Conductivity 0,863 0,019 µs/cm Organic Matter 7,49 0,064 % Kjeldahl Nitrogen 0,41 0,006 % N Available Phosphorus 392, mg/kg SL 5 ph 8,7 0,08 UpH Conductivity ,67 µs/cm Calcium 23,1 1,45 %CaO Phosphorus ,31 mg/kg Potassium ,68 mg/kg Available Boron 0,991 0,07 mg/kg Available Magnesium ,34 mg/kg Equivalent Calcium Carbonate 56,7 0,69 % CaCO3 Exchange Bases 6,77 2,64 q Na/100 g m SL 9 Kjeldahl Nitrogen 0,25 0,006 % N Available Potassium 170, mg/kg Oxidable Organic Matter 5,27 0,25 % Calcium 4,26 0,128 % CaO Sodium 0,16 0,025 % Na2O Arsenic 36,6 0,989 mg/kg Copper 36, mg/kg Chromium 115, mg/kg SL 11 Copper 22,1 0,72 mg/kg Iron mg/kg Lead 25,5 0,85 mg/kg ph 7,46 0,039 U ph Available Magnesium 106 5,3 mg/kg Total Phosphorus 0,14 0,004 % P2O5 SL 16 Cadmium 15,2 0,507 mg/kg Copper 21,2 1,054 mg/kg Chromium 55,2 2,34 mg/kg Mercury 4,3 0,178 mg/kg Nickel 26,6 1,494 mg/kg Lead 33,7 1,475 mg/kg Zinc 65,4 1,9 mg/kg Arsenic 20,6 0,65 mg/kg SL 17 Total Phosphorus 0,271 0,010 % P2O5 Iron ,207 mg/kg Magnesium 1,22 0,044 % MgO Sodium 0,045 0,002 % Na2O SL 18 Calcium 34,47 1,083 % CaO Conductivity ,224 µs/cm Manganese 160 5,569 mg/kg Potassium 0,36 0,025 % K2O ph 8,47 0,043 UpH SL 19 Cadmium 33,1 1,581 mg/kg Chromium 17,8 2,055 mg/kg Nickel 8,5 0,700 mg/kg page 13 of 16

38 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 ABSORPTION SOLUTION part nr. I Part nr. Analyte Property value Uncertainty Units SC 2 Antimony 100,2 3,99 µg/l Cadmium 172,6 5,73 µg/l Cobalt 80,4 3,02 µg/l Chromium 251 8,27 µg/l SC 3 Fluorhidric acid 0,700 0,020 mg/l SC 4.1 Sulphur Dioxide (SO2) 200 2,72 mg/l SC 4.2 Arsenic µg/l Cobalt µg/l Chromium µg/l Vanadium µg/l SC 5.1 Hydrochloric acid (HCl) mg/l SC 5.2 Cadmium µg/l Copper µg/l Tin µg/l Lead µg/l SC 6.1 Hydrofluoric acid (HF) 1 0,027 mg/l SC 6.2 Antimony µg/l Manganese µg/l Nickel µg/l Zinc µg/l SC 7.1 Hydrochloric acid (HCl) 74,6 0,999 mg/l SC 7.2 Cadmium µg/l Copper µg/l Manganese µg/l Nickel µg/l SC 8.1 Hydrofluoric acid (HF) 0,51 0,014 mg/l SC 8.2 Arsenic 316 8,59 µg/l Antimony 142 4,58 µg/l Cadmium 109 3,36 µg/l Vanadium 241 7,21 µg/l SC 9.1 Sulphur Dioxide (SO2) 343 4,1 mg/l SC 9.2 Cobalt 83,4 2,68 µg/l Chromium 174 5,02 µg/l Lead 231 6,39 µg/l Zinc 148 4,51 µg/l SC 10.1 Hydrofluoric acid (HF) 1,07 0,029 mg/l SC 10.2 Cadmium 37,7 1,15 µg/l Arsenic 134 4,06 µg/l Antimony 107 3,44 µg/l Copper 263 7,13 µg/l SC 11.1 Hydrochloric acid (HCl) 59,9 0,779 mg/l SC 11.2 Chromium 291 7,86 µg/l Manganese 182 5,23 µg/l Lead 172,7 4,99 µg/l Vanadium 79 2,5 µg/l SC 12.1 Sulphur Dioxide (SO2) 570,3 5,85 mg/l SC 12.2 Cobalt 203 5,77 µg/l Tin 108 3,59 µg/l Nickel 59,6 1,82 µg/l Zinc ,52 µg/l SC 13.1 Fluorhidric acid 1,2 0,016 mg/l SC 13.2 Cadmium 74,86 0,659 µg/l Arsenic 167,36 1,616 µg/l Antimony 216,7 2,574 µg/l Copper 256,08 2,215 µg/l SC 14.1 Chloridric acid (HCl) 84,21 0,487 mg/l SC 14.2 Chromium 94,73 0,601 µg/l Manganese 111,3 0,599 µg/l Lead 79,36 0,62 µg/l Vanadium 158,73 0,974 µg/l SC 15.1 SO2 465,15 4,487 mg/l SC 15.2 Cobalt 91,93 0,56 µg/l Tin 160,1 1,475 µg/l Nickel 191,05 1,324 µg/l Zinc 263,9 2,482 µg/l page 14 of 16

39 APPENDIX TO THE PHYSICHAL-CHEMICAL REFERENCE MATERIAL CATALOGUE version: February 2011 PCBs part nr. I Part nr. Analyte Property value Uncertainty Units PCB 1 PCBs (sum) ,55 mg/kg PCB 2 PCBs (sum) ,67 mg/kg PCB 3 PCBs (sum) 17,7 1,18 mg/kg PCB 4 PCBs (sum) 191 9,75 mg/kg PCB 5 PCBs (sum) 71,6 1,73 mg/kg PCB 6 PCBs (sum) ,78 mg/kg PCB 7 PCBs (sum) 202 5,13 mg/kg PCB 8 PCBs (sum) ,44 mg/kg page 15 of 16