Nitrogen limitation as a driver of genome size evolution in a group of karst plants

Transcription

1 Supporting information Nitrogen limitation as a driver of genome size evolution in a group of karst plants Ming Kang, Jing Wang, Hongwen Huang Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China Table S1 Raw data of the 100 populations of Primulina in this study Species Genome C N P C : N N : P C : P size (pg) (mg g -1 ) (mg g -1 ) (mg g -1 ) P. baishouensis P. bicolor P. bipinnatifida P. brachytricha P. bullata P. cavicola P. chizhouensis P. cordifoli P. cordifolia P. danxiaensis P. depressa P. dongguanica P. eburnea P. eburnea P. fimbrisepala P. glandulosa P. gueilinensis P. gueilinensis var brachycarpa P. guihaiensis P. hedyotidea P. heterotricha P. hochiensis P. huaijiensis P. juliae P. langshanica P. latinervis P. laxiflora P. leiophylla

2 P. lepingensis P. leprosa P. liboensis P. liguliformis P. lijiangensis P. linearicalyx P. linearifolia P. lobulata P. longgangensis P. longicalyx P. longii P. longistyla P. lunglinensis P. lungzhouensis P. luochengensis P. lutea P. mabaensis P. macrodonta P. medica P. moii P. mollifolia P. nandanensis P. napoensis P. obtusidentata P. ophiopogoides P. orthandra P. parvifolia P. pinnatifida P. pseudoeburnea P. pterppoda P. pulchurfolia P. qingyuanensis P. renifolia P. repanda P. repanda var guilinensis P. ronganensis P. rongshuiensis P. roseo-alba P. sclerophylla P. shouchengensis P. sinensis P. spinulosa P. subrhomboidea P. subrhomboidea var. tribracteata

3 P. subulata P. swinglei P. tabacum P. tenuifolia P. tenuituba P. tiandengensis P. tribracteata P. varicolor P. verecunda P. villosissima P. wentsaii P. xiuningensis P. xiziae P. yangchunensis P. yongxingensis P. yungfuensis P. sp.nov P. sp.nov P. sp.nov P. sp.nov P. sp.nov P. sp.nov P. sp.nov P. sp.nov P. sp.nov P. sp.nov P. sp.nov P. sp.nov

4 Table S2 Summary of genome size (2C DNA content) in relation to leaf nutrient contents under regression models of ordinary least squares (OLS) and phylogenetic generalized least squares (PGLS). The values of log-likelihood (Lh), slope, R 2 and P are indicated. Statistically significant relationships are in bold (P<0.05) PGLS OLS Lh Slope R 2 P Lh Slope R 2 P C (mg g -1 ) N (mg g -1 ) P (mg g -1 ) C: N C: P N: P

5 Table S3 Summary of leaf N concentration in relation to latitude and bioclimatic variables under the regression model of phylogenetic generalized least squares (PGLS) Slope R 2 P Latitude ( N) BIO 1 = Annual mean temperature BIO 2 = Mean diurnal range (mean of monthly (max temp min temp)) BIO 3 = Isothermality (BIO2/BIO7) (*100) BIO 4 = Temperature seasonality (standard deviation *100) BIO 5 = Max temperature of warmest month BIO 6 = Min temperature of coldest month BIO 7 = Temperature annual range (BIO5-BIO6) BIO 8 = Mean temperature of wettest quarter BIO 9 = Mean temperature of driest quarter BIO 10 = Mean temperature of warmest quarter BIO 11 = Mean temperature of coldest quarter BIO 12 = Annual precipitation BIO 13 = Precipitation of wettest month BIO 14 = Precipitation of driest month BIO 15 = Precipitation seasonality (coefficient of variation) BIO 16 = Precipitation of wettest quarter BIO 17 = Precipitation of driest quarter BIO 18 = Precipitation of warmest quarter BIO 19 = Precipitation of coldest quarter

6 Fig. S1 Sampling locations of the 100 populations representing 99 species of Primulina in south China. The map was made with Natural Earth (Free vector and raster map naturalearthdata.com).