Kluwer Academic Publishers

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1 Kluwer Academic Publishers P.O. Box 17,3300 AA Dordrecht, The Netherlands Dear Reader We would very much appreciate receiving your suggestions and criticisms on the Plant Tissue Culture Manual. They will be most helpful during our preparations for future supplements. Would you please answer the questions listed below, and send your comments with any further suggestions you may have, to Dr. M. Brewis at the above-mentioned address. Thank you for your assistance! Dr M. Brewis Publisher PLANT TISSUE CULTURE MANUAL I. What errors have you found? (list page numbers and describe mistakes) 2. What protocols do you find to be confusing or lacking in detail? (list chapter numbers and page numbers and describe problems) 3. What protocols do you feel should be replaced in future supplements with newer (better) methods? 4. What new topics or other material would you like to see included in future supplements? Please print or type your answers in the space below, and continue overleaf. Name: Address: Date:

2 Plant Tissue Culture Manual Supplement 1, February 1992 INSTRUCTIONS FOR SUPPLEMENT 1 Preliminary pages Replace: Table of Contents Section A Add after Chapter A 7 : Chapter A8: Chapter A9: D. Valvekens, M. van Lijsebettens and M. van Montagu/Arabidopsis regeneration and transformation (root explant system) A.D. Krikorian and D.L. Smith/Somatic embryogenesis in carrot (Daucus carota) Chapter AlO: G. Spangenberg and H.-U. Koop/Low density cultures: microdroplets and single-cell nurse cultures Section B Add qfter Chapter BB: Chapter B9: Section C Add after Chapter C5: ChapterC6: Section D Add after Chapter D6: Chapter D7: e.a. Rhodes and D.W. Gray/Transformation and regeneration of maize protoplasts M.e. Coleman and W. Powell/Virus elimination and testing P.K. Saxena and J. King/Isolation and uptake of plant nuclei End matter Replace: Index

3 PLANT TISSUE CULTURE MANUAL SUPPLEMENT 1

4 PLANT TISSUE CULTURE MANUAL Supplement 1 Edited by : K. LINDSEY Plant Cell & Molecular Biology Group Leicester Biocentre. University of Leicester. U.K. Springer Science+Business Media, B.Y. 1992

5 Library of Congress Cataloglng-in-Publication Data Plant tissue culture manual : fundamentals and applications / edited by K. Lindsey. p. cm. Includes bibliographical references and index. ISBN (acid-free paper) 1. Plant tissue culture Laboratory manuals. I. Lindsey, K. QK725.P T dc ISBN ISBN ISBN (ebook) DOI / Printed on acid-free paper All Rights Reserved 1992 Springer Science+Business Media Dordrecht Originally published by Kluwer Academic Publishers in 1992 Softcover reprint of the hardcover 1st edition 1992 No part of the material protected by this copyright notice may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording or by any information storage and retrieval system, without written permission from the copyright owner.

6 Contents Preface SECfION A: BASIC TECHNIQUES - CELL & TISSUE CULTURE OF MODEL SPECIES 1. Media preparation O.L. Gamborg, Fort Collins, USA 2. The initiation and maintenance of callus cultures of carrot and tobacco R.D. Hall, Wageningen, The Netherlands 3. The initiation and maintenance of plant cell suspension cultures R.D. Hall, Wageningen, The Netherlands 4. Shoot cultures and root cultures of tobacco I.F. Topping and K. Lindsey, Leicester, UK 5. Somatic embryogenesis in orchardgrass M.E. Hom, Davis, USA 6. Arabidopsis regeneration and transformation (leaf & cotyledon explant system) R. Schmidt and L. Willmitzer, Berlin, Germany 7. Arabidopsis protoplast transformation and regeneration B. Damm and L. Willmitzer, Berlin, Germany *8. Arabidopsis regeneration and transformation (root explant system) D. Valvekens, M. van Lijsebettens and M. van Montagu, Gent, Belgium *9. Somatic embryogenesis in carrot (Daucus carota) A.D. Krikorian and D.L. Smith, New York, USA * 10. Low density cultures: microdroplets and single-cell nurse cultures G. Spangenberg, Ladenburg, Germany, and H.U. Koop, Munich, Germany SECfION B: TISSUE CULTURE & TRANSFORMATION OF CROP SPECIES l. Embryogenic callus, cell suspension and protoplast cultures of cereals I.K. Vasil and V. Vasil, Gainesville, USA Included in Supplement I.

7 2. Transformation and regeneration of rice protoplasts J. Kyozuka and K. Shimamoto, Yokohama, Japan 3. Transformation and regeneration of orchardgrass protoplasts M.E. Horn, Davis, USA 4. Transformation and regeneration of oilseed rape protoplasts D. Rouan and P. Guerche, Versailles, France 5. Regeneration and transformation of potato by Agrobacterium tumefaciens R.G.F. Visser, Wageningen, Netherlands 6. Transformation of tomato with Agrobacterium tumefaciens S. McCormick, Albany, USA 7. Regeneration and transformation of sugarbeet by Agrobacterium tumefaciens K. Lindsey, P. Gallois and C. Eady, Leicester, UK 8. Regeneration and transformation of apple (Malus pumila Mill.) DJ. James and A.M. Dandekar, Davis, USA *9. Transformation and regeneration of maize protoplasts c.a. Rhodes and D.W. Gray, Palo Alto, USA SECTION C: PROPAGATION & CONSERVATION OF GERMPLASM 1. Clonal propagation of orchids Y. Sagawa, Honolulu, USA 2. Clonal propagation of palms B. Tisserat, Pasadena, USA 3. Clonal propagation of conifers T.A. Thorpe and I.S. Harry, Alberta, Canada 4. Cytological techniques A. Karp, Long Ashton, UK 5. Restriction fragment analysis of somaciones R.H. Potter, As, Norway *6. Virus elimination and testing M.C. Coleman and W. Powell, Dundee, UK Included in Supplement I.

8 SECTION D: DIRECT GENE TRANSFER & PROTOPLAST FUSION 1. Gene transfer by particle bombardment T.M. Klein, S. Knowlton and R. Arentzen, Wilmington, USA 2. Transformation of pollen by particle bombardment D. Twell, T.M. Klein and S. McCormick, Albany, USA 3. Electrical fusion of protoplasts M.G.K. Jones, Murdoch, Australia 4. Cybrid production and selection E. Galun and D. Aviv, Rehovot, Israel 5. Fluorescence-activated analysis and sorting of protoplasts and somatic hybrids D.W. Galbraith, Lincoln, USA 6. RFLP analysis of organellar genomes in somatic hybrids E. Pehu, Helsinki, Finland *7. Isolation and uptake of plant nuclei P.K. Saxena and J. King, Saskatoon, Canada * Index * Included in Supplement I.