Raphanus sativus L. Raphaiol. Thin Layer Chromatography R f

Transcription

1 Raphaiol Thin Layer Chromatography R f

2 Tissue Cultivation of Plant and Identification of Raphaiol Alkaloid of Extraction of The Seeds, Explants, Callus and produced Plants from tissue Cultivation Asst. Lect. Taghreed Nawaf Ahmed Department of Biology College of Education / Mosul University Abstract: The present study managed the initiation of callus from leaves, stems and roots of the sterilized Seeds using the solid supplied with various concentrations of growth regulators (, ). The root parts showed the highest percentage of 100% in the presence of 1.0 mg/liter of and. Then the stem parts in which the callus initiation estimated at 98%, and the leaves parts with 88% percentage initiating which is done in the same medium. The callus of the stem and the leaves of has showed a high ability of regeneration of shoots in the same medium. Moreover, the results of isolation and identification of Raphaiol alkaloid from the seeds, the plant parts (leaves, stems, and roots) and the produced callus from them and the leaves of the plants produced from callus by using the thin layer chromatography showed an isolated spot from each tested sample.these samples showed perfect conformity with the rate flow (R f ) and perfect and approximate conformity with the rates flow of the standard Raphaiol alkaloids spot (the control sample amounted to)=(0.59). Bajaj, 1998 Sinapine Raphanin Raphaiol Raphaiol

3 Raphaiol Gutierrez, 2004 TLC Murashige & Skoog, 1962 NaOCl * : * : O

4 Naphthalene Benzyl adenine Acetic Acid

5 Raphanus sativas L. AlDaody Electro Thermal Rotary Vacuum Evaporator NH 4 OH 1500 r.p.m Harborne, 1973 NH 4 OH Raphaiol 1984 Wagner Whatman No.1

6 G Thin Layer Chromatography (TLC) Drummond Scientific Tank MeOH : NH 4 OH Co.U.S.A Harborne, 1976 UV R f R f Mikes, 1979 R f

7 NaOCl Mohammad & Collin, Badigannavar & Kuruvinashetti, Negrutiu, 1978 Totipotency

8 Raphanus 2,4D+ Kin sativus L. Kin 2,4D

9 A B C A B C C B A A B C Raphanus sativus L.

10 AlSafadi, 1990 Phillips, 1985 B A D C Hartmann, Regeneration et al., 2002 Street, 1977 Hartmann, 2002 Ozyigit, 2007

11 A B C D A B C D

12 Dragen droff TLC TLC Thin layer chromatography Andreu, 1982 Sarin, 2005 Rf R f Rate of flow Rf Andreu, 1982 R f

13 Larkin & Scowcroft, 1981 R f A B C D Raphaiol TLC A B C D

14 A B C D Raphaiol TLC A B C D

15 Reference 1. Bajaj Y.P.S. (1998). Biotechnology for improvement of medicinal plants. Symposium on Plant Biotechnology As a Tool For Exploitation of Mountain Lands., 457: Gutierrez, R. M. P. & Perez, R. L. (2004). (Radish): Their Chemistry and Biology. The scientific world Journal 4: Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth bioassay with tobacco tissue culture, physiol. plant. 15: Helianthus annuus L 8. AlDaody, A. G. K. (1998). Chemical study on some Iraqi plants, (Ph.D), University of Mosul, Iraq. 9. Harborne, J. B. (1973). phytochemical methods; Vol. I, p: Wagner, H. B.; Ladt, S. & Zgainski, E. M. (1984). "Plant drug analysis a thin layer chromatography atlas". Springer Verlage. Berlin. 11. Harborne, J. B. O. (1976). "Phytochemical Methods". John Willey and Sons. Inc., New York. 12. Mikes, O. (1979). "Chromatographic and Allied Methods". John Willey and Sons, Inc., New York Badigannavar, A. M. & Kuruvinashetti, M. S. (1996). Bractas an Explant for callus induction and shoot bud formation in sunflower (Helianthus annus L.) Agricultural sciences, 19:25, Mohammad, A. M. S. & Collin, H. A. (1979). Growth and invertase activity of sugar beet callus. New Phytol., 82:

16 18. Negrutiu, I.; Jacobs, M. & Dorina, G. (1978). Some factors controlling in vitro morphogenesis of (Arabidopsis Thaliana). Z. pflanzen Physiol; 86: AlSafadi, B. & Simon, P. W. (1990). The Effects of gamma irradiation on the growth and cytology of carrot (Daucus carota). Tissue culture Environ. Expt. Bot., 30: Phillips, G. C. & Hustenberger, J. F. (1985). Organogenesis in pepper tissue cultures. Plant cell. Tiss. Org. Cult., 4: Hartmann, H. T.; D. E. Kester; F. T. Davies & R. L. Geneve (2002). Plant Propagation Principles and Practices. 7 th ed., Perntice Hall, Inc. New Jersey. USA. 22. Street, H. E. (1977). "Plant Tissue and Cell Culture Black Well Scientific Publication". Oxford, London Edinburgh, Melbourne. Nigella sativa L. 24. Ozyigit, I, I; Gozkirmizi, N, and Semiz, B. D. (2007). Genotype de pended callus in duction and shoot regeneration in sun flower (Helianthus, annuus, L.) Afri. J. Biotechnol. Vol. 6(13), PP Andreu, J. M. & Timasheff, S. N. (1982). Biochemistry; 2: 21(3): Sarin, R. (2005). Biotechnology, 4(2): Larkin, P. J. & Scowcroft, W. R. (1981). Theor. Appl Genet ; 60:

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