Welcome to BIOL 572: Recombinant DNA techniques

Transcription

1 Lecture 1: 1 Welcome to BIOL 572: Recombinant DNA techniques Agenda 1: Introduce yourselves Agenda 2: Course introduction Agenda 3: Some logistics for BIOL 572 Agenda 4: Q&A section

2 Agenda 1: Introduce yourselves Bioluminescence and its application Lecture 1: 2 Bacteria Jellyfish Tobacco expressing firefly luciferase

3 Agenda 1: Introduce yourselves Bioluminescent dinoflagellates Lecture 1: 3 10 µm Day phase Night phase Scintillons (in white) are light-emitting organelles Their numbers change on a daily basis Red fluorescence comes from chlorophyll

4 Agenda 2: Course introduction The Trio who discovered the DNA double helix Lecture 1: 4 The crystal structure of the B-DNA dodecamer C-G-C-G-A-A-T-T-C-G-C-G DNA-protein-DNA recognition: The lac repressor-operator system

5 Agenda 2: Course introduction Lecture 1: 5 Isolation, electrophoresis, and hybridization of nucleic acids PCR, DNA sequencing, and mutagenesis Genomic and cdna Library construction and screening Protein expression in prokaryotic and eukaryotic cells Characterization of protein-dna or RNA interactions using yeast genetic technology and gel retardation Reporter gene assays RNA interference Transgenic organisms and gene therapy DNA microarray analysis

6 Agenda 2: Course introduction Lecture 1: 6 DNA sequencing analysis and bioinformatics 2003 H. Sapiens (human), 3200 Mb, genes 2002 S. pombe (fission yeast), 12.5 Mb, 5468 genes 2001 A. thaliana (plant), 125 Mb, 25,500 genes 2000 D. melanogaster (fruit fly), 180 Mb, 13,600 genes 1998 C. elegans (worm), 97 Mb, 15,000 genes 1997 E. coli (bacteria), 4.6 Mb, 4403 genes 1996 S. cerevisiae (budding yeast), 12.2 Mb, 7813 genes 1995 H. Influenzae (bacteria), 1.8 Mb, 1740 genes 1990 Genome sequencing began (viruses), Mb In 1977, rapid DNA sequencing methods were invented

7 Agenda 3: Some logistics for BIOL 572 Lecture 1: 7 Instructor: Drs. Liyun Liu and Dan Odom Lecture: Monday and Wednesday, 12:00-12:50 P.M., Science 2225 Laboratory Section 1(LL): Monday and Wednesday 2:00-4:50 P.M., Science 4019 Laboratory Section 2 (DO): Monday and Wednesday 9:00-11:50 A.M., Science 4019 Office hours: Tuesdays, 10:00 A.M.-3:00 P.M. or by appointment, Office Locations: Science 4114 or Contact information: Liyun.Liu@csun.edu No Textbooks!! Course requirements and methods of evaluation:. An attendance is mandatory for both lecture and laboratory sections. In this course, it is necessary for the students not only to read the assigned reading materials, and to perform well in the examinations and quizzes, but also to prepare weekly laboratory reports, to do a research project, and to present the research results in posters and presentations. The evaluation will be based on the students increasing understanding of key concepts and their continuing improvement in experimental skills. Grading criteria: Your performance in the lecture and laboratory will be graded separately. The points that can be earned from each category as shown below will be converted into grades A-F with the plus/minus system. Homework and lab reports are due Next Monday.

8 Agenda 3: Some logistics for BIOL 572 Lecture Total 100 Laboratory Total 100 Lecture 1: : A 90-94: A : B : B 75-79: B : C : C 60-64: C : D : D 45-49: D- <45: Failure Attendance: 20 Quizzes: 15 Assignments: 5 Mid-term exam: 25 Final examination: 30 A review article: 5 Attendance: 20 Participation: 5 Maintenance of lab notes: 5 Laboratory reports: 50 Poster and presentation: 5 Project : 15 Your should start working on your review article and project after the first three weeks.

9 Agenda 4: Q&A section Lecture 1: 9

10 Lecture 1: 10 Answer questions based on the figure. 1.Write down the sequence of the double-stranded DNA using common abbreviations for four nucleotides, G, A, T, and C. 2. Assuming that the double-stranded DNA is separated into two single strands by heat denaturation, can you determine whether the two single strands have the same mass or not? Why? 3. How many picograms does one such molecule weigh? Reading assignment:: Watson and Crick 1953 Nature Paper