CELL BIOLOGY, BIOINFORMATICS AND SYSTEMS BIOLOGY BIO160 (GU); UMF012 (Chalmers) schedule 2010
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1 CELL BIOLOGY, BIOINFORMATICS AND SYSTEMS BIOLOGY BIO160 (GU); UMF012 (Chalmers) schedule 2010 Head of course: Markus Tamas (MT) Cell and Molecular Biology-Microbiology Medicinaregatan 9C Lundberg Laboratory (Room 1415) Göteborg Phone: Responsible for the bioinformatics part Tore Samuelsson Department of Medical Biochemistry and Cell Biology Medicinareg. 9A Göteborg, Sweden Phone: Lecturers and assistants: Lasse Ottosson (LO) Loubna Bendrioua (LB) Madelene Palmgren (MP) Roja Babazadeh (RB) Lectures, group discussions and lab practicals will be at the Medical Hill (Medicinareberget) in Göteborg. For those of you who might have difficulties in finding the lecture room 2303 (on Medicinaregatan 9C located on 3 rd floor above Lundberg Café) we will see each other at the entrance to Lundberg Laboratory at on September 1 st. Literature: Essential cell biology - An introduction to the molecular biology of the cell (2 nd Edition) Alberts et al., 2004; Garland Science Marks on the course: The final mark on the course will be the result of 1) the presentation of the function of a gene, 2) written labreports, 3) written summaries of research seminars and 4) the exam. The balance between the tasks in the final mark will be 1) 12%, 2) 19%, 3) 19%, and 4) 50%. Lab practicals and the preparation and discussions regarding the lab practicals are compulsory. Also the research seminars and the project presentations are compulsory. The group discussions are optional and it is up to you to attend and get help from the assistants, however, it is highly recommended that you take part in these events. 1
2 w1 (35) INTRODUCTION TO CELLS 1/9 Wednesday (room: 2303) Welcome and introduction to the course Lecuture (MT) - The cell cells under the microscope; cell sizes: cell organisation; prokaryote; eukaryote Chapter 1 (p. 1-16) Lecture (MT) - Cell diversity unity and diversity of cells; genetic model organisms; E.coli; yeast; C.elegans; Arabidopsis; Drosophila; mouse Chapter 1 (p ) 2/9 Thursday (room: 1412) Lecture (MT) - Chemical bonds chemical bonds; ionic bonds; covalent bonds; water Chapter 2 (p ) Lecture (MT) - Molecules in cells molecules in cells; building blocks; macromolecules Chapter 2 (p ) 3/9 Friday (room: Maj Bring) Lecture (MT) - Thermodynamics catalysis; enzymes; thermodynamics Chapter 3 (p ) Lecture (MT) - Biosynthesis activated carriers; ATP; NADH and NADPH; biosynthesis Chapter 3 (p ) w2 (36) CHEMICAL COMPONENTS OF CELLS AND BASIC CELL METABOLISM 7/9 Tuesday (room: Gösta Sandels) Lecture (LB) - Energy generation Catabolism; glycolysis; acetyl-coa; citric acid cycle Chapter 13 (p ) Lecture (LB) - Regulation of metabolism electron transport; storage; regulation of metabolism Chapter 13 (p ) (room: Gösta Sandels) Preparation for practical I 8/9 Wednesday (Lundberg Laboratory; kurslab 5) Practical I - Cellular growth 9/9 Thursday (Lundberg Laboratory; kurslab 5) Practical I 2
3 9/9 Thursday (room: 1412) Lecture (LO) - DNA replication DNA; genes; structure; replication; DNA polymerase; Chapter 6 (p ) Lecture (LO) - DNA repair DNA repair; mutations Chapter 6 (p ) (Lundberg Laboratory; kurslab 5) Practical I - Cellular growth (cont.) 10/9 Friday (room: Maj Bring) Lecture (MP) - Protein structure shape and structure; alpha-helix; beta-sheet; cross linkage Chapter 4 (p ) Lecture (MP) - Protein properties affinities; antibodies; enzyme kinetics; allosteric enzymes Chapter 4 ( ) (room: Maj Bring) How to write a labreport?! w3 (37) PROTEIN, DNA AND CHROMOSOMES 13/9 Monday (room: A404) Lecture (LO) - DNA technology I restriction endonucleases; gel electrophoresis; DNA sequencing; DNA hybridisation; Chapter 10 (p ) Lecture (LO) - DNA technology II DNA cloning, cdna libraries; PCR; DNA engineering Chapter 10 (p ) 14/9 Tuesday (room: A428) Lecture (LB) - Transcription from DNA to RNA; transcription; RNA polymerase; RNA processing; introns; Chapter 7 (p ) Lecture (LB) - Translation from RNA to protein; translation; ribosomes; trna; codons; RNA and the origin of life Chapter 7 (p ) (room: A428) Preparation for practical II 15/9 Wednesday (Lundberg Laboratory; kurslab 5) Practical II - Protein characterisation 3
4 16/9Thursday (Lundberg Laboratory; kurslab 5) 8-17 Practical II - Protein characterisation 17/9 Friday (room: Gösta Sandels) Lecture (RB) - Chromosomes the structure of eucaryotic chromosomes; nucleosomes; replication start sites Chapter 5 (p ) Lecture (RB) - Gene regulation gene regulatory proteins; general transcription factors; combinatorial control Chapter 8 ( ) w4 (38) CELLULAR INTEGRATION 20/9 Monday (room: A428) Lecture (MT) - Genetic variation genetic variation in bacteria; transformation; homologous recombination; bacterial mating; plasmids Chapter 9 (p ) Lecture (MT) - Viruses and transposable elements genetic variation in eucaryotes; gene duplication; transposable elements; viruses; retroviruses; sexual reproduction; meioses Chapter 9 (p ) 21/9 Tuesday (room: Karl Kylberg) Lecture (MP) - Membrane structure the lipid bilayer; membrane proteins; detergents Chapter 11 (p ) Lecture (MP) - Membrane transport carrier proteins; active transport; osmotic balance; ion channels; nerve cells Chapters 12 (p ) (room: Karl Kylberg) 22/9 Wednesday (room: A428) Lecture (LO) Mitochondria biogenesis; mitochondrial genome; ATP generation Chapter 14 (p ) Lecture (LO) - Chloroplasts biogenesis; chloroplast genome; photosynthesis Chapter 14 (p ) 23/9 Thursday (room: A428) Lecture (MT) - Protein sorting signal sequences; chaperones Chapter 15 (p ) Lecture (MT) - Secretion vesicular transport; protein modification; ER; Golgi Chapter 15 (p ) 4
5 24/9 Friday (room: Maj Bring) Lecture (RB) - Intracellular signalling receptors; nitric oxide; signalling cascades Chapter 16 (p ) Lecture (RB) - G-protein linked receptors cyclic AMP; phospholipase C; tyrosine kinases Chapter 16 (p ) w5 (39) CELL COMMUNICATION AND CELL DIVISION 28/9 Tuesday (room: 1411) Preparation for practical III (room: 1411) 29/9 Wednesday (room: A404) Lecture (MT) - Cytoskeleton microtubules; actin filaments; myosin Chapter 17 (p ) Lecture (MT) - Cell cycle control and cell death cyclin dependent protein kinases; checkpoints; apoptosis Chapter 18 (p ) 29/9 Wednesday (Lundberg Laboratory; kurslab 5) Practical III - DNA characterisation 30/9 Thursday (room: Maj Bring) Lecture (MT) - Cell division mitosis; mitotic spindle; cytokinesis Chapter 19 (p ) Lecture (MT) Genetics, Meiosis, Heredity Meiosis, Molecular basis of heredity Chapter 20 (p ) 30/9 Thursday (Lundberg Laboratory; kurslab 5) Practical III - DNA characterisation (cont.) 1/10 Friday (room: 1411) Lecture (MT) - Tissues Extracellular matrix; collagen; integrins; gap junctions; cancer; development Chapter 21 (p ) 5
6 w6 (40) RESEARCH SEMINARS 5/10 Tuesday (room: A428) Helpdesk for gene presentation (room: A428) 5/10 Tuesday (room: A428) Research seminar (Dr. Bodil Nordlander) + discussion 6/10 Wednesday (room: 1412) Research seminar (Dr. Bodil Nordlander) + discussion 7/10 Thursday (room: A404) Research seminar (Dr. Jonas Warringer) + discussion W7 (41) PROJECT PRESENTATION 12/10 Tuesday (room: Åke Göransson) Oral presentations 13/10 Wednesday (room: 1411) Oral presentations 14/10 Thursday (room: Maj Bring) Final discussion practicals (room: Maj Bring) W8 (42) SELF STUDY + WRITING OF REPORTS & SUMMARIES + EXAM 20/10 Wednesday (room: 1412) Questions 22/10 Friday Examination Info later Usually in the V-House (Väg och Vatten) at Chalmers The room will be posted in the entrance to the V-House 6
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