surpass all possibilities [ Cortecs 2.7 µm columns ]

Transcription

1 surpass all possibilities [ Cortecs.7 µm columns ]

2 [ CORTECS.7 µm COLUMNS ] A SOLID-CORE PARTICLE COLUMN THAT LIVES UP TO ITS POTENTIAL.

3 C 8 + C 8 HILIC.7 μm SOLID-CORE PARTICLE COLUMNS FOR HPLC AND UHPLC SEPARATIONS Unleash the power of your laboratory s instrumentation. CORTECS.7 μm Solid-Core Particle Columns are designed to maximize performance of HPLC or UHPLC systems by maximizing efficiency, resulting in enhanced resolution and peak capacity at HPLC- and UHPLC-optimized pressures. Whether you are developing a quality control method or transferring an LC separation, CORTECS Columns will exceed your LC column expectations.

4 [ CORTECS.7 µm COLUMNS ] TRUTH BEHIND INCREASED EFFICIENCY WITH SOLID-CORE PARTICLES Efficiency gains of solid-core particle columns were previously credited to the shorter diffusion path in solid-core particles versus fully-porous particles. This shorter diffusion path resulted in faster mass transfer kinetics. However, for analytes of low molecular weight which have high diffusion rates, mass transfer kinetics have been found to play a minimal role in efficiency improvement. Recent research suggests that solid-core particles demonstrate improved performance by lowering the three terms of the van Deemter equation: Solid-core particles may pack more uniformly lowering the A term Their lower particle porosity reduces axial diffusion lowering the B term Their solid core may improve heat transfer, diminishing radial temperature gradients lowering the C term HETP A B υ C υ G. Guiochon and F. Gritti, J. Chrom. A 8, 0, Waters CORTECS Columns are designed to maximize efficiency, throughput, and performance. Additionally, they fulfill the promise of solid-core particle columns.

5 SETTING THE STANDARD FOR REPRODUCIBILITY Waters cutting-edge manufacturing facilities are dedicated to producing columns that maximize laboratory performance. As a primary manufacturer of silica and hybrid particles, we are able to continually monitor and control the manufacturing process over the lifetime of a product. Controlled manufacturing processes ensure you receive the best, most reliable column possible for consistent performance year after year. CORTECS Columns are created using state-of-the-art column packing techniques and bonding and endcapping processes. The result is consistent, reproducible performance, and long column lifetimes. Excellent Batch-to-Batch Reproducibility for,.7 µm Columns System: ACQUITY UPLC H-Class System with ACQUITY PDA 0.0 Batch A Column: Mobile phase:,.7 µm,. x 0 mm (p/n 8007) Acetonitrile/. mm ammonium formate, ph (/, v/v) Flow rate: Injection volume: µl 0. ml/min Column temp.: 0 C 0.0 Batch B Detection: Sample diluent: UV at nm Mobile phase 0.0 Compounds:. Uracil ( µg/ml). Promethazine (.0 µg/ml). Amitriptyline (8.0 µg/ml). Butylparaben (. µg/ml). Naphthalene (0 µg/ml) Batch C 0.0 Batch D min Separations obtained using columns containing four different batches of bonded material demonstrate the excellent reproducibility that can be expected from CORTECS Columns, assuring the long-term reproducibility of your analytical method. Stability at Low ph: Hour Exposure to 0.% TFA at 0 C,.7 µm +,.7 µm Competitor A, Solid-Core C 8,.7 µm Competitor B, Solid-Core C 8,. µm Competitor C, Solid-Core C 8,. µm % Retention Loss for Methyl Paraben In this test, the loss in retention of the neutral marker methyl paraben indicates bonded phase loss due to acid hydrolysis. CORTECS Columns resist bonded phase loss due to novel bonding and endcapping processes resulting in good column lifetimes.

6 [ CORTECS.7 µm COLUMNS ] EMPOWERING METHOD DEVELOPMENT There are several factors to consider when developing a method. Parameters that influence your separation include mobile phase composition, temperature, and column chemistry. With three phases to choose from, CORTECS.7 µm Columns offer both reversed-phase and orthogonal HILIC selectivity, giving you flexibility and the power to develop methods for challenging separations. With different particle characteristics and innovative charged surface modifications, CORTECS.7 µm Columns are suitable for use in a wide variety of applications. CORTECS CHEMISTRY CHARACTERISTICS C 8 + C 8 HILIC Chemistry O O Si O O O Si O General purpose, high-efficiency, General purpose, high-efficiency, High-efficiency column reversed-phase column. A positively reversed-phase column. Balanced designed for retention of Intended Use charged surface delivers excellent peak shape for basic compounds retention of acids, bases, and neutrals at low- and mid-range ph. extremely polar analytes. Offers orthogonal selectivity at low ionic strength acidic vs. C 8 columns. mobile phases. Ligand Type Trifunctional C 8 Trifunctional C 8 None Surface Charge Modification + None None Endcap Style Proprietary Proprietary None Carbon Load.7%.% Unbonded Ligand Density. µmol/m.7 µmol/m N/A ph Range Temperature Limits* Low ph = C High ph = C Low ph = C High ph = C Low ph = C High ph = C Performance Standard Neutrals QCRM Neutrals QCRM HILIC QCRM Application Standard Reversed-Phase QCRM Reversed-Phase QCRM HILIC QCRM * Recommended temperature limits when operating at the extremes of the ph range. Higher temperatures may be used when the ph is not near the limits. Implement the appropriate Waters Quality Control Reference Material (QCRM) into your workflow to benchmark system performance and gain confidence in your results. For more information, visit

7 IMPROVED PEAK SHAPE AND INCREASED MS SENSITIVITY FOR BASIC ANALYTES C is a general purpose, high-efficiency, reversed-phase column which features a positively charged surface modification. Charged surface technology utilizes a controlled, low-level surface charge for enhanced selectivity and exceptional peak shape for basic compounds when using acidic, low-ionic strength mobile phases such as formic acid. Benefits of + Columns include unique column selectivity with industry-leading reproducibility, exceptional peak shape and loading capacity without the need for ion-pair reagents, and improved signal-to-noise performance in LC and LC-MS applications. Superior Peak Shape for Low Level Impurity Analysis 00 Competitor Solid-Core C 8,. µm Pressure max : 0 psi : SIR of Channel ES+ TIC (Impurity at 0.% spike).8e7 +,.7 µm Pressure max : 70 psi : SIR of Channel ES+ TIC (Impurity at 0.% spike).8e7 % S/N Ratio: 0% Increase in S/N Ratio S/N Ratio: min min.0e-.e- () PDA Ch nm@.8nm Range: 8e- () PDA Ch nm@.8nm Range:.0e-.e- Imipramine % Decrease in Peak Width Imipramine.0e-.e-.0e- Impurity at 0.% spike PW.% : 0. min Impurity at 0.% spike PW.% : 9 min.0e min min Comparative separations may not be representative in all applications. HPLC-UV/MS analysis of the low-ionic basic antidepressant imipramine reveals a low level impurity. Using a +,.7 µm Column designed for use with low ionic strength acidic mobile phases results in narrower peaks and improved signal-to-noise. Data conditions Columns:.0 x 0 mm; Mobile phase A: 0.% formic acid in water; Mobile phase B: 0.% formic acid in acetonitrile; Gradient: to %B in. minutes; Flow rate: 0.8 ml/min; Column temperature: 0 C; Detection: UV@ nm and ESI+ MS; Injection volume: 0 µl; Compounds: imipramine (0. mg/ml), impurity at 0.% spike (0. µg/ml). 7

8 [ CORTECS.7 µm COLUMNS ] EXCELLENT RESOLUTION OF ACIDS, BASES, AND NEUTRALS C 8 The C 8 ligand is the most popular choice in method development because of its stability and ability to retain a variety of analytes. is a traditional C 8 -bonded phase which exhibits balanced retention of acids, bases, and neutrals at low- and mid-range ph. It provides superb resolution and retention for complex mixtures. Analysis of Acids, Bases, and Neutrals System: ACQUITY UPLC H-Class System with ACQUITY PDA Detector Fully-Porous C 8, µm,. x 0 mm Peak capacity: 00 0 Column:,.7 µm,. x 0 mm (p/n ) Mobile phase A:. mm ammonium formate ph Mobile phase B: Acetonitrile Gradient: Time (min) %A %B Curve Initial min 7 8 9,.7 µm,. x 0 mm Peak capacity: 0 0 min Comparative separations may not be representative in all applications. Flow rate:.0 ml/min Injection volume: 0 µl Column temp.: 0 C Detection: UV at nm Sample diluent:. mm ammonium formate ph /acetonitrile (9/, v/v) Compounds:. Caffeine (0 µg/ml) 7. Propranolol (0 µg/ml). -Nitrobenzoic Acid ( µg/ml) 8. -Nitrophenol (0 µg/ml). Metoprolol (00 µg/ml) 9. Amitriptyline (0 µg/ml). Papaverine ( µg/ml) 0. Diethylphthalate ( µg/ml). -Chlorobenzoic Acid ( µg/ml). Fenoprofen (00 µg/ml). Pyrenesulfonic Acid (9 µg/ml). Dipropylphthalate ( µg/ml) Analysis of a column characterization mix of acids, bases, and neutrals, using a,.7 µm,. x 0 mm Column on an ACQUITY UPLC H-Class System. The improved efficiencies of CORTECS.7 µm Columns versus fully-porous particle columns produce sharper, narrower peaks resulting in higher peak capacity and increased sensitivity. 8

9 SUPERIOR RESOLUTION AND RETENTION OF POLAR ANALYTES HILIC CORTECS HILIC,.7 µm Columns are designed specifically for the retention of very polar compounds using hydrophilicinteraction chromatography (HILIC). HILIC is a complementary chromatographic technique to reversed-phase that can provide an orthogonal separation mode for mixtures of ionizable and very polar compounds. The combination of acetonitrile-rich mobile phase and polar, unbonded CORTECS HILIC stationary phase offers additional chromatographic benefits such as increased mass spectrometry response and direct injection of organic extracts from common sample preparation techniques, such as solid-phase extraction (SPE), liquid-liquid extraction (LLE), and protein precipitation (PPT). Retention of Polar Morphine Metabolites Compounds:. -Acetylmorphine (0. mg/ml). Morphine (0. mg/ml). Morphine-N-Oxide (0. mg/ml). Morphine-β-glucuronide (0. mg/ml) System: Column: Mobile phase A: Mobile phase B: Alliance HPLC with 998 Photodiode Array Detector CORTECS HILIC,.7 µm,. x 0 mm (p/n 80079) 0 mm ammonium formate in 0% acetonitrile/9.87% water/0.% formic acid 0 mm ammonium formate in 90% acetonitrile/9.87% water/0.% formic acid Gradient: Time (min) %A %B Curve 0. CORTECS HILIC,.7 μm,. x 0 mm Pressure max :97 psi Initial Flow rate:.99 ml/min Injection volume:. µl 0.0 Column temp.: Detection: Sample: Sample diluent: 0 C UV at 80 nm Morphine Metabolites Mobile phase B min Using HILIC gradient test conditions and an Alliance HPLC System, a mix of morphine and its polar metabolites were analyzed using a CORTECS HILIC,.7 µm,. x 0 mm Column. To learn more about Waters Comprehensive Guide to HILIC (Hydrophilic Interaction Chromatography), visit 9

10 [ CORTECS.7 µm COLUMNS ] REDUCE BACKPRESSURE WITHOUT SACRIFICING EFFICIENCY CORTECS.7 µm Columns offer the unique advantage of operating at lower backpressure without sacrificing efficiency or resolution. Additionally, CORTECS.7 µm Columns are more efficient than columns packed with fully-porous particles of equivalent size. Advantages of combining lower operating backpressure and high efficiency include: Increased resolution using longer columns Improved utilization and performance of existing HPLC and UHPLC equipment Faster flow rate operation for improved throughput Efficiency Advantages of CORTECS.7 µm Columns Efficiency (USP) Top of Class Efficiency +,.7 µm Fully-Porous C 8,. µm Competitor A Solid-Core C 8,. µm Competitor B Solid-Core C 8,.7 µm CORTECS.7 µm Columns exhibit excellent efficiency compared to similarly-sized, fullyporous and solid-core particle columns. Data conditions Columns:. x 0 mm; Mobile phase: water/acetonitrile (/7, v/v); Column temperature: 0 C; Detection: UV@ nm; Injection volume: 0. µl; Compounds: acenaphthene (00 µg/ml), octanophenone (00 µg/ml). Comparative separations may not be representative in all applications. Flow Rate (ml/min) Linear Velocity (cm/min). x 0 mm x 0 mm x 0 mm Backpressure Advantages of CORTECS.7 µm Columns Column Pressure (psi) ,.7 µm Fully-Porous C 8,. µm Competitor A Solid-Core C 8,. µm Competitor B Solid-Core C 8,.7 µm Linear Velocity (cm/min) at % lower backpressure than the competitor s. µm solid-core particle column CORTECS.7 µm Columns offer a % reduction in operating backpressure without sacrificing efficiency. Data conditions Columns:. x 0 mm; Mobile phase: water/acetonitrile (/7, v/v); Column temperature: 0 C; Detection: UV@ nm; Injection volume: 0. µl; Compounds: acenaphthene (00 µg/ml), octanophenone (00 µg/ml). Comparative separations may not be representative in all applications. Flow Rate (ml/min). x 0 mm x 0 mm x 0 mm

11 The combination of higher efficiency with lower backpressure culminates in the flexibility to run methods at HPLC- and UHPLC-optimized backpressures, using longer column lengths and higher flow rates to further improve efficiency, resolution, and throughput. Increased Efficiency and Resolution for Estradiols with,.7 µm Columns Compounds:. Estradiol (0 µg/ml). Ethinyl estradiol (0 µg/ml). Estrone (0 µg/ml). Levonorgestrel (0 µg/ml) R s. R s.8 R s.9 Fully-Porous C 8, µm,. x 0 mm Pressure: 900 psi N peak : min 0.0 Fully-Porous C 8,. µm,. x 0 mm Pressure: 900 psi 0.0 N peak : R s.9 R s. R s min R s. R s. R s 8.,.7 µm,. x 0 mm Pressure: 00 psi N peak : min Comparative separations may not be representative in all applications. Increased efficiency (N) and resolution (R s ) of estradiols can be achieved using a,.7 µm Column at HPLCand UHPLC-optimized backpressures. Data conditions Column:,.7 µm,. x 0 mm (p/n ); Mobile phase: 0.% formic acid in water/acetonitrile (/); Flow rate:.0 ml/min; Column temperature: 0 C; Detection: UV@0 nm; Injection volume: 0 µl. Waters Column Advisor recommends the most appropriate columns for your specific application requirements. For help with choosing a column, visit

12 [ CORTECS.7 µm COLUMNS ] BOOST YOUR LABORATORY S PRODUCTIVITY CORTECS.7 µm Columns enable you to achieve faster separations without sacrificing performance. Shorter-length columns can be used to reduce analysis time of the original method and increase the number of samples analyzed. With higher flow rates, gradient times can be further reduced, increasing the speed of analysis. Increased Throughput and Speed using,.7 µm Columns Peak capacity: Full-Porous C 8, µm,. x 00 mm 0.0 Flow rate: ml/min Injection volume: µl min Peak capacity: 0.80,.7 µm,. x 0 mm 0.0 Higher Throughput Flow rate: ml/min Injection volume:. µl min Peak capacity: 0.80 Compounds: 0.0 Greater Speed. Carbadox (0. mg/ml). Oxolinic acid (0. mg/ml). Sulfaquinoxaline (0. mg/ml). Nalidixic acid (0.mg/mL),.7 µm,. x 0 mm Flow rate: ml/min Injection volume:. µl min Comparative separations may not be representative in all applications. Throughput was increased x by replacing a fully-porous, µm, 00 mm length column with a shorter length CORTECS Column. A further x time savings was then achieved by doubling the flow rate without sacrificing peak capacity. Data conditions Columns:,.7 µm,. x 0 mm (p/n 80077); Fully-Porous C 8, µm,. x 00 mm; Mobile phase A: 0.% formic acid in water; Mobile phase B: 0.% formic acid in acetonitrile; Gradients: to 0%B in,, or minutes; Column temperature: 0 C; Detection: UV@ nm. The CORTECS Family of and.7 µm Solid-Core Particle Columns improves the performance of your laboratory s LC systems by increasing resolution while improving sample throughput.

13 SIMPLE METHOD TRANSFER In the contemporary world of multi-national and multi-site laboratories whose LC platforms may be sourced from different vendors, Waters recognizes the need for versatility and ease of method transfer. It is essential that robust methods be created quickly and easily while being compatible with a wide range of chromatographic systems. With particle sizes ideal for HPLC, UHPLC, and UPLC platforms, you can be assured a simple, and seamless transfer with consistent results across different particle sizes and column configurations. USP Method Transfer of Abacavir with Time and Solvent Savings Fully-Porous C 8, µm,. x 0 mm Flow rate:.00 ml/min Injection volume: 8 µl System: Alliance HPLC Original method Compounds:. Descycleopropylabacavir. Abacavir. R,R Trans abacavir. O-(-Chloro-,-diaminopyimidnyl)-abacavir. O-t-Butyl-abacavir min,.7 µm,. x 7 mm Flow rate:.8 ml/min Injection volume: µl System: Alliance HPLC Transfer to.7 µm X faster method X less solvent Comparative separations may not be representative in all applications min ,.7 µm,. x 7 mm Flow rate:.8 ml/min Injection volume: µl System: ACQUITY UPLC H-Class Compatible with HPLC, UHPLC, and UPLC systems min CORTECS UPLC C 8,,. x 0 mm Flow rate: 0. ml/min Injection volume: 0. µl System: ACQUITY UPLC H-Class Transfer to 9X faster method X less solvent min Methods developed on µm fully-porous columns can be scaled and transferred to shorter.7 µm columns. For further efficiency gains and productivity improvements, sub--µm UPLC columns can be used, enabling greater flexibility in method consistency when transitioning between laboratories within an organization or to contract partners. Data conditions Mobile phase A: 0.% trifluoroacetic acid in water; Mobile phase B: 8% methanol in water; Geometrically-scaled gradients (i.e., same column volumes per gradient step): Fully-Porous C 8, µm,. x 0 mm column: to 0%B in. minutes and 0 to 90%B in.8 min;,.7 µm,. x 7 mm column (p/n 80077): to 0%B in. minutes and 0 to 90%B in.0 minutes;,,. x 0 mm column: to 0%B in. minutes and 0 to 90%B in. minutes; Column temperature: 0 C; Detection: UV@ nm.

14 [ CORTECS.7 µm COLUMNS ] VANGUARD COLUMN PROTECTION The often complex sample matrices encountered in pharmaceutical, natural product, environmental, and industrial chemical analysis prematurely shorten analytical column lifetime and degrade chromatographic performance. Waters VanGuard Column Protection Products help improve analytical column performance by removing particulate and chemical contamination that may be present in the mobile-phase stream. VanGuard Pre-columns and VanGuard Cartridges are uniquely designed and optimized to protect and prolong analytical column lifetimes without compromising chromatographic performance. Each available CORTECS VanGuard configuration is ideally suited for the physical and chemical protection for CORTECS UPLC and HPLC Columns. KEY FEATURES AND BENEFITS: Feature First guard column for UPLC and UHPLC applications Patent-pending, ultra-low volume design Manufactured using Waters column hardware, particles, and sorbent chemistries Connects directly to Waters HPLC, XP, and UPLC columns Benefit Guaranteed compatibility with pressures up to 8,000 psi Minimal chromatographic effects Superior HPLC, UHPLC, and UPLC column protection and performance Leaks and connection voids are eliminated Minimal Chromatographic Effects with VanGuard Cartridges 0.0 CORTECS C8,.7 µm,. x 00 mm Column without guard column Peak I.D. Without Vanguard Cartridge Peak Capacity With VanGuard Cartridge Sulfathiazole. Sulfamerazine 8. Sulfamethazine 0. Sulfamethoxypyrizadine 8. Sulfachloropyrizadine 9. Sulfamethoxazole 7 7. Sulfafurazole CORTECS C8,.7 µm,. x 00 mm Column VanGuard C8,.7 µm,.9 x mm Cartridge Column 7 System: Mobile phase A: Mobile phase B: Alliance e9 with e89 nm 0. % formic acid in water 0. % formic acid in acetonitrile 0.0 Gradient: Time Flow rate %A %B (min) (ml/min) min Column temp.: 0 C Concentration: 0 µg/ml Economical VanGuard Cartridge Columns can be used to extend analytical column lifetime without compromise to separation performance. Injection volume: µl

15 ORDERING INFORMATION CORTECS.7 µm Columns Chemistry Particle Size Dimension Part No. Pack Part No. Pack +.7 µm. x 0 mm µm. x 0 mm µm. x 7 mm µm. x 00 mm µm. x 0 mm µm.0 x 0 mm µm.0 x 0 mm µm.0 x 7 mm µm.0 x 00 mm µm.0 x 0 mm µm. x 0 mm µm. x 0 mm µm. x 7 mm µm. x 00 mm µm. x 0 mm µm. x 0 mm µm. x 0 mm µm. x 7 mm µm. x 00 mm µm. x 0 mm µm.0 x 0 mm µm.0 x 0 mm µm.0 x 7 mm µm.0 x 00 mm µm.0 x 0 mm µm. x 0 mm µm. x 0 mm µm. x 7 mm µm. x 00 mm µm. x 0 mm CORTECS HILIC.7 µm. x 0 mm CORTECS HILIC.7 µm. x 0 mm CORTECS HILIC.7 µm. x 7 mm CORTECS HILIC.7 µm. x 00 mm CORTECS HILIC.7 µm. x 0 mm CORTECS HILIC.7 µm.0 x 0 mm CORTECS HILIC.7 µm.0 x 0 mm CORTECS HILIC.7 µm.0 x 7 mm CORTECS HILIC.7 µm.0 x 00 mm CORTECS HILIC.7 µm.0 x 0 mm CORTECS HILIC.7 µm. x 0 mm CORTECS HILIC.7 µm. x 0 mm CORTECS HILIC.7 µm. x 7 mm CORTECS HILIC.7 µm. x 00 mm CORTECS HILIC.7 µm. x 0 mm VanGuard Column Protection (Guard Columns) Chemistry Particle Part No. Part No. Size. x mm, /pk.9 x mm, /pk VanGuard Cartridge.7 µm VanGuard Cartridge.7 µm CORTECS HILIC VanGuard Cartridge.7 µm Universal VanGuard Cartridge Holder Recommended for. mm and.0 mm i.d. columns Recommended for. mm i.d. columns CORTECS.7 µm Columns Method Validation Kits (MVK)* Chemistry Particle Size Dimension Part No. +.7 µm. x 0 mm µm. x 0 mm µm. x 7 mm µm. x 00 mm µm. x 0 mm µm.0 x 0 mm µm.0 x 0 mm µm.0 x 7 mm µm.0 x 00 mm µm.0 x 0 mm µm. x 0 mm µm. x 0 mm µm. x 7 mm µm. x 00 mm µm. x 0 mm µm. x 0 mm µm. x 0 mm µm. x 7 mm µm. x 00 mm µm. x 0 mm µm.0 x 0 mm µm.0 x 0 mm µm.0 x 7 mm µm.0 x 00 mm µm.0 x 0 mm µm. x 0 mm µm. x 0 mm µm. x 7 mm µm. x 00 mm µm. x 0 mm 8007 CORTECS HILIC.7 µm. x 0 mm 8007 CORTECS HILIC.7 µm. x 0 mm 8007 CORTECS HILIC.7 µm. x 7 mm 8007 CORTECS HILIC.7 µm. x 00 mm CORTECS HILIC.7 µm. x 0 mm CORTECS HILIC.7 µm.0 x 0 mm CORTECS HILIC.7 µm.0 x 0 mm CORTECS HILIC.7 µm.0 x 7 mm 8007 CORTECS HILIC.7 µm.0 x 00 mm 8007 CORTECS HILIC.7 µm.0 x 0 mm 8007 CORTECS HILIC.7 µm. x 0 mm 8007 CORTECS HILIC.7 µm. x 0 mm 8007 CORTECS HILIC.7 µm. x 7 mm 8007 CORTECS HILIC.7 µm. x 00 mm CORTECS HILIC.7 µm. x 0 mm *Each kit contains three columns from three batches of material. Quality Control Reference Materials (QCRM) Description Part No. Reversed-Phase QCRM 800 Neutrals QCRM 8000 HILIC QCRM 8007 QDa QCRM 8007 LCMS QCRM 8009 For a full list of Waters Analytical Standards and Reagents, visit CORTECS.7 µm Columns CORTECS UPLC Columns

16 SALES OFFICES: Australia Austria Belgium and Luxembourg Brazil 788 Canada China 8 Czech Republic Denmark Finland France Germany Hong Kong Hungary 0 08 India Ireland 8 00 Israel Italy Japan Korea Mexico The Netherlands Norway Poland Portugal Puerto Rico Russia/CIS / Singapore Spain Sweden 8 00 Switzerland Taiwan UAE 97 8 UK US Waters Corporation Maple Street Milford, MA 077 U.S.A. T: F: Waters, The Science of What s Possible, Alliance, CORTECS, ACQUITY, UPLC, and ACQUITY UPLC are registered trademarks of Waters Corporation. CSH is a trademark of Waters Corporation. All other trademarks are property of their respective owners. 0 Waters Corporation. Printed in the U.S.A. February EN Rev C SC-SIG

17 ultimate efficiency unleashed [ Cortecs columns ]

18 [ CORTECS UPLC COLUMNS ] A SUB--µm SOLID-CORE PARTICLE COLUMN THAT LIVES UP TO ITS POTENTIAL.

19 C 8 + C 8 HILIC SOLID-CORE PARTICLE COLUMNS THAT DELIVER ULTIMATE EFFICIENCY AND PERFORMANCE With our unique understanding of how to harness the power of sub--µm particles, Waters brings you the latest addition to our family of sub--µm UltraPerformance LC Columns. Based on solid-core particle technology, CORTECS Columns enable you to achieve new levels of efficiency and performance. Whether you re trying to resolve complex mixtures or maintain resolution while increasing throughput, CORTECS Columns will surpass ALL expectations.

20 [ CORTECS UPLC COLUMNS ] T RUTH BEHIND INCREASED EFFICIENCY WITH SOLID-CORE PARTICLES Why do solid-core particles achieve higher column efficiency? Initial explanation previously credited the shorter diffusion path in solid-core particles versus fully-porous particles. A shorter diffusion path results in faster mass transfer kinetics. However, for analytes of low molecular weight which have high diffusion rates, mass transfer kinetics have been found to play a minimal role in efficiency improvement. Modern findings suggest that solid-core particles improve performance by lowering each of the three terms of the van Deemter equation: Solid-core particles may pack more uniformly lowering the A term Their lower particle porosity reduces axial diffusion lowering the B term Their solid core may improve heat transfer, diminishing radial temperature gradients lowering the C term HETP A B υ C υ G. Guiochon and F. Gritti, J. Chrom. A 8, 0, Waters CORTECS Columns are designed to maximize efficiency, throughput, and performance as well as to fulfill the promise of solid-core particle columns.

21 ACHIEVE A NEW EFFICIENCY STANDARD Due to the characteristic duality of their morphology, solid-core particles hold the potential for higher efficiency when compared to fully-porous particles of a similar size. However, achievement of this theoretical potential requires the use of ultra-low dispersion column hardware and optimal packing techniques. By using unique column hardware and proprietary packing equipment and methodologies, Waters has set the efficiency standard for UPLC Columns. Backed by over a decade of knowledge and expertise in manufacturing sub--μm particle columns, CORTECS UPLC Columns deliver the full benefit of solid-core particle technology. DESIGNED AND CONSTRUCTED FOR MAXIMUM EFFICIENCY AND THROUGHPUT Efficiency Advantage of CORTECS UPLC Columns 9% higher efficiency than solid-core C8,.7 µm column at optimal flow rate Same efficiency as fully-porous.7 µm column at up to x the flow rate Efficiency (USP) CORTECS UPLC C8+, 000 Fully-Porous C8,.7 µm 000 Competitor Solid-Core C8,.7 µm Flow Rate (ml/min) Comparative separations may not be representative in all applications. CORTECS UPLC Columns offer higher efficiency than columns containing sub--µm fully-porous particles as well as those containing.7 µm competitor solid-core particles. They also give chromatographers the option to more than double the throughput of their current sub--µm column separation while maintaining a similar efficiency. Data conditions Columns:. x 0 mm; Analyte: acenaphthene; Mobile phase: 7: (v/v) acetonitrile/water; Temperature: 0 C.

22 [ CORTECS UPLC COLUMNS ] MAXIMUM EFFICIENCY FOR MAXIMUM RESOLUTION As described in this equation, resolution depends on three variables: the efficiency of the column/system (N), the selectivity between compounds (α), and the retention factor (k) of the analyte. N (α-) k α (k+) RS Resolution increases in proportion to the square root of efficiency. So, the higher efficiency of CORTECS UPLC Columns gives you increased resolution when analyzing complex samples compared to fully-porous columns. Improved Resolution of Local Anesthetics on,. μm Columns Column:,,. x 0 mm (p/n 80070) Fully-Porous HILIC,.7 µm,. x 0 mm 0.0 Fully-Porous HILIC,.7 µm,. x 0 mm 0. USP Resolution, :. System: ACQUITY UPLC I-Class with ACQUITY PDA Mobile phase: 90:0 acetonitrile/0 mm ammonium formate with 0.% formic acid Separation mode: Isocratic Flow rate: 0.8 ml/min Injection volume:.0 µl Column temp.: 0 C Detection: UV at nm Sample diluent: 7: acetonitrile/methanol with 0.% formic acid Sample concentration: 00 µg/ml Lidocaine. Butacaine. Tetracaine. Procaine. Procainamide 0. USP Resolution, :..0 min Compounds:,,. x 0 mm min Comparative separations may not be representative in all applications. Improved resolution for a separation of local anesthetics using a Column compared to a fully-porous column, using the same method conditions.

23 FASTER SEPARATIONS FOR GREATER THROUGHPUT Another benefit of CORTECS UPLC Columns is that they enable you to increase sample throughput with comparable or better peak capacities, simply by raising the flow rate of the method. Alternatively, the high efficiency of CORTECS UPLC Columns can enable the use of a shorter column length compared to the original separation, while maintaining similar peak capacities and providing faster re-equilibration time. This increased speed of analysis without sacrificing separation performance gives you the ability to run more samples in the same amount of time and obtain results faster while decreasing the costs of analysis and lab operation. Faster Separation and Similar Peak Capacity at Double the Flow Rate Fully-Porous C8,.8 μm,. x 0 mm Flow rate: 0. ml/min.8 µm,. x 0 mm Gradient time:. min Runtime: min Peak capacity: peaks per minute (gradient) min 0.8 Compounds: Peak capacity: peaks per minute (gradient) Flow rate:.0 ml/min,. x 0 mm Gradient time:. min Runtime: min 0.. CORTECS UPLC C8+,. μm,. x 0 mm. Faster separation with similar peak capacity for sulfa drugs using a CORTECS UPLC C8+ Column (p/n: 8007) at double the flow rate, compared to a fully-porous column. Data conditions System: ACQUITY UPLC H-Class; UV detection: nm, scaling the gradient to account for the change in flow rate; Sample concentration: 0 µg/ml min. Sulfathiazole. Sulfamerazine. Sulfamethazine. Sulfamethoxypyridazine. Sulfachloropyridazine. Sulfamethoxazole 7. Sulfasoxazole Comparative separations may not be representative in all applications. Higher Throughput With Shorter Column Length Competitor Solid-Core C8,.7 µm,. x 00 mm min CORTECS UPLC C8+,,. x 0 mm Gradient time:.min Runtime: min Peak capacity: 7 0 peaks per minute (gradient) Gradient time: 8. min Runtime: min Peak capacity: 7 peaks per minute (gradient) Higher throughput with similar peak capacity for sulfa drugs using a 0 mm length CORTECS UPLC C8+ Column (p/n: 8007), compared to a 00 mm length competitor solid-core particle column. Data conditions System: ACQUITY UPLC H-Class; UV detection: nm, scaling the method to account for the change in column length; Sample concentration: 0 µg/ml Compounds:. Sulfathiazole. Sulfamerazine. Sulfamethazine. Sulfamethoxypyridazine. Sulfachloropyridazine. Sulfamethoxazole 7. Sulfasoxazole. min Comparative separations may not be representative in all applications. 7

24 [ CORTECS UPLC COLUMNS ] REPRODUCIBILITY YOU CAN DEPEND ON With more than fifty years of experience in the separation sciences, Waters chromatographic media sets the standard for quality. As a primary manufacturer of chromatographic media, Waters monitors and controls each step of the manufacturing process to maintain unparalleled reproducibility. The result is consistent performance year-after-year the hallmark of reliability. Excellent Batch-to-Batch Reproducibility for CORTECS UPLC C8 Columns System: ACQUITY UPLC H-Class System with ACQUITY PDA 0.0 CORTECS UPLC C8,,. x 0 mm (p/n ) Acetonitrile/. mm ammonium formate, ph (/, v/v) 0. ml/min Column: 0.0 Batch A Mobile phase: Injection volume: µl Column temp.: 0 C Detection: Flow rate: UV at nm 0.0 Sample diluent: 0.0 Batch B Mobile phase Compounds:. Uracil ( µg/ml). Promethazine (.0 µg/ml). Amitriptyline (8.0 µg/ml). Butylparaben (. µg/ml). Naphthalene (0 µg/ml) Batch C Separationsobtained using columns containing four different batches of bonded material demonstrates the solid reproducibility that can be expected from 0.0 CORTECS UPLC Columns, assuring the long-term reproducibility of your analytical method. 0.0 Batch D min 0

25 EMPOWERING METHOD DEVELOPMENT There are several factors to consider when developing a method. Parameters that influence your separation include mobile phase composition, temperature, and column chemistry. With three phases to choose from, CORTECS UPLC Columns offer both complementary and orthogonal selectivity, giving you flexibility and power to develop methods faster. With different particle characteristics including innovative charged surface modifications, CORTECS UPLC Columns are suitable for use in a wide variety of applications. CORTECS UPLC CHEMISTRY CHARACTERISTICS C8+ Chemistry HILIC General purpose, high-efficiency, High-efficiency column designed O O O Si O Si O O General purpose, high-efficiency, Intended Use C8 reversed-phase column. A positively reversed-phase column. Balanced for retention of extremely polar charged surface delivers excellent retention of acids, bases, and analytes. Offers orthogonal peak shape for basic compounds neutrals at low- and mid-range ph. selectivity vs. C8 columns. at low ionic strength acidic mobile phases. Ligand Type Trifunctional C8 Trifunctional C8 None Surface Charge Modification + None None Endcap Style Proprietary Proprietary None Carbon Load.7%.% Unbonded Ligand Density. µmol/m.7 µmol/m N/A ph Range Low ph = C Low ph = C Low ph = C High ph = C High ph = C High ph = C Performance Standard Neutrals QCRM Neutrals QCRM HILIC QCRM Application Standard Reversed-Phase QCRM Reversed-Phase QCRM HILIC QCRM Temperature Limits* * Recommended temperature limits when operating at the extremes of the ph range. Higher temperatures may be used when the ph is not near the limits. 9

26 [ CORTECS UPLC COLUMNS ] ENHANCED PEAK SHAPE AND LOADING CAPACITY FOR BASIC ANALYTES The greatest benefit of CSH Technology is improved peak shape and loading capacity for basic analytes using low-ionic strength, acidic-mobile phases. This technology imparts a low-level positive charge to the CORTECS particle surface which enables the use of low-ionic strength formic acid mobiles in place of ion-pairing reagents such as trifluoroacetic acid. Improved Peak Shape of Basic Analytes with CORTECS UPLC C8+ Columns 0.0 Competitor Solid-Core C8,.7 µm,. x 0 mm 0.0 Column: Peak capacity: 7 CORTECS UPLC C8+,,. x 0 mm (p/n 8007) Competitor Solid-Core C8, µm,. x 0 mm System: ACQUITY UPLC H-Class with ACQUITY PDA Mobile phase A: 0.% formic acid in water Mobile phase B: 0.% formic acid in acetonitrile Gradient: 8 % B over minutes Flow rate: 0. ml/min Injection volume:.0 µl Column temp.: 0 C Detection: UV at nm min 0.0 CORTECS UPLC C8+,,. x 0 mm Peak capacity: 0.0 Sample concentration: 0 µg/ml 0.0 Compounds:. Nordoxepin. Protriptyline. Nortriptyline. Amitriptyline. Trimipramine min Comparative separations may not be representative in all applications. Improved peak shape of basic compounds using a CORTECS UPLC C8+ Column, compared to a competitor C8 solid-core particle column results in much higher peak capacity for the CORTECS UPLC Column separation using the same method conditions. Implement the appropriate Waters Quality Control Reference Material (QCRM) into your workflow to benchmark system performance and gain confidence in your results. For more information, visit 0

27 EXCELLENT RESOLUTION AND RETENTION WITH CORTECS UPLC C 8 COLUMNS Most chromatographers choose C8 ligands for their excellent retention and stability. The CORTECS C8 material is a traditional C8 bonded phase that exhibits balanced retention of acids, bases, and neutrals at low- and mid-range ph, and provide superb efficiency, resolution, and retention for complex analyte mixtures. Greater Resolution for Synthetic Cannabinoids on a CORTECS UPLC C8 Column 00 8 Fully-Porous C8,.7 µm,. x 00 mm Pressureinitial:,00 psi System: ACQUITY UPLC Column: CORTECS UPLC C8,. μm,. x 00 mm (p/n800709) Column temp.: 0 C Injection volume: 0 μl Flow rate: 0. ml/min Mobile phase A: 0.% formic acid in Milli-Q water Mobile phase B: 0.% formic acid in acetonitrile Gradient: Time (min) Initial CORTECS UPLC C8,,. x 00 mm min 8 Pressureinitial:,800 psi 0 %A %B Curve 7 9 Compounds: min Comparative separations may not be representative in all applications. UPLC-MS/MS analysis of synthetic cannabinoids and metabolites at 0 ng/ml using a CORTECS UPLC C8 Column, compared to a Fully-Porous C8,.7 µm,. x 00 mm column at 0. ml/min. The analysis was performed on an ACQUITY UPLC System with a Xevo TQD Mass Spectrometer, using the same method for both columns. Co-elution is evident on the fully-porous column for peaks /, 7/8 and 9/0 (isobaric), but these are sufficiently resolved for quantitation on the CORTECS UPLC C8,. μm Column.. AM. RCS-, M0. RCS-, M. AM 8. JWH-07 -butanoic acid metabolite. JWH-07 -hydroxybutyl metabolite 7. JWH-08 -pentanoic acid metabolite 8. JWH-07 (+/-) -hydroxybutyl metabolite 9. JWH--0 0hydroxypentyl metabolite 0. JWH-08 (+/-) -hydroxypentyl metabolite Waters Column Advisor recommends the most appropriate columns for your specific application requirements. For help with choosing a column, visit

28 [ CORTECS UPLC COLUMNS ] RETENTION OF POLAR ANALYTES WITH COLUMNS Hydrophilic-interaction chromatography (HILIC) is a separation mode that can be used to improve the retention of extremely polar analytes. Columns are unbonded solid-core particles specifically designed for this application. HILIC uses mobile phases with a high concentration of organic solvent which enables effective desolvation of analytes in the MS source, resulting in improved MS response and sensitivity. Retention and Resolution of Neurotransmitters on Column ACh 00 Ch System: ACQUITY UPLC Column:,. μm,. mm x 00 mm (p/n 80070) Mobile phase A: 00 mm ammonium formate, ph Mobile phase B: Acetonitrile Gradient: Time (min) %A Initial t-miaa iso-ach HA % t-mha Flow rate: 0. ml/min Column temp.: C Sample temp.: C Injection volume: 0 μl Run time:. min %B Curve min UPLC-MS/MS analysis of neurotransmitters at pg/ml in artificial cerebrospinal fluid (acsf) using a CORTECS UPLC HILIC,,. x 00 mm Column and a Xevo TQ-S Mass Spectrometer. These compounds are highly polar and will typically be poorly retained in reversed-phase LC. To learn more about Waters Comprehensive Guide to HILIC (Hydrophilic Interaction Chromatography), visit

29 MATCHING COLUMN CONFIGURATIONS WITH LC SYSTEMS Selecting an appropriate column for your system is very important. The dispersion (extra-column band spreading) of the system will have considerable impact on the observed performance of the column, especially for columns of smaller internal diameter. As system dispersion decreases, peaks become narrower and taller and the efficiency of a separation increases. High-efficiency CORTECS Columns can be paired with any LC system, but when paired with an ultra-low dispersion instrument such as the ACQUITY UPLC I-Class Systems, you can experience new levels of performance. Impact of Particle Size and System Dispersion on Efficiency 0.07 System: UHPLC (band spread: µl) 0.0 Competitor Solid-Core C8,. µm,.0 x 0 mm Competitor UHPLC 0.0 ACQUITY UPLC I-Class with ACQUITY PDA; USP Res, :. 0.0 Column: USP Plate Count (peak ): CORTECS UPLC C8+,,. x 0 mm (p/n 8007) 0.0 Competitor Solid-Core C8,. µm,.0 x 0 mm min 0.0 Isocratic Mobile phase: : water/acetonitrile with 0.% formic acid Flow rate: 0. ml/min (,. x 0 mm) 0.9 ml/min (. µm,.0 x 0 mm) ACQUITY UPLC I-Class (band spread: 7 µl) 0.0 Injection volume: CORTECS UPLC C8,,. x 0 mm Separation mode:.0 µl (,. x 0 mm).0 µl (. µm,.0 x 0 mm) 0.0 USP Res, :. USP Plate Count (peak ): Column temp.: 0 C Detection: UV at 0 nm Sample concentration: 0 µg/ml min The separation of estradiols on a. µm competitor solid-core particle column using a UHPLC system with µl system band spread, compared to the same separation on a CORTECS UPLC Column using an ACQUITY UPLC I-Class System with 7 µl system band spread. Band Spread Values for Modern Chromatographic Systems and the Recommended Column i.d. for the Best Column Performance Column i.d. (mm) * Determined at flow rate of 0. ml/minute using acetonitrile/water (0/0, v/v) as the mobile phase and caffeine (0 µg/ml) (p/n Band Spread (µl)* st Choice nd Choice 70000) as the sample. A 0. µl injection was used and the HPLC 0..0 peak was detected at 7 nm using a data rate of 80 Hz or highest UHPLC.0. UPLC 8..0 System available for the system. T he peak width was measured at.% of peak height.

30 [ CORTECS UPLC COLUMNS ] VERSATILITY IN SUB--µm PARTICLE DESIGN Scientists are often challenged by the need to analyze mixtures of compounds that vary in their polarity, molecular weight, functionality, and complexity. While screening columns with different ligands is an essential strategy in method development, choosing the particle with the appropriate attributes for the separation is even more crucial. This is why we have added CORTECS Solid-Core Particles to our growing family of innovative particles. CORTECS SOLID-CORE PARTICLE High efficiency Increased throughput at similar efficiency* Higher performance at same backpressure* UPLC to HPLC scalability * Compared to fully-porous particles CSH TECHNOLOGY Superior peak shape BEH TECHNOLOGY for basic analytes Exceptional loading ph stability capacity Mobile phase and temperature versatility UPLC to HPLC scalability Unparalleled UPLC to HPLC scalability HSS TECHNOLOGY Maximum retention Enhanced selectivity UPLC to HPLC scalability

31 CORTECS UPLC Columns ORDERING INFORMATION CORTECS UPLC Columns Method Validation Kits (MVK)* CORTECS UPLC Columns Chemistry Particle Size Dimension Part No. 700 CORTECS UPLC C8+. x 0 mm CORTECS UPLC C8+. x 0 mm x 7 mm CORTECS UPLC C8+. x 7 mm x 00 mm CORTECS UPLC C8+. x 00 mm x 0 mm CORTECS UPLC C8+. x 0 mm x 0 mm CORTECS UPLC C8+.0 x 0 mm x 0 mm CORTECS UPLC C8+.0 x 0 mm CORTECS UPLC C8+.0 x 7 mm CORTECS UPLC C8+.0 x 7 mm CORTECS UPLC C8+.0 x 00 mm CORTECS UPLC C8+.0 x 00 mm CORTECS UPLC C8+.0 x 0 mm CORTECS UPLC C8+.0 x 0 mm CORTECS UPLC C8. x 0 mm CORTECS UPLC C8. x 0 mm 8007 CORTECS UPLC C8. x 0 mm CORTECS UPLC C8. x 0 mm x 7 mm Dimension Part No. Pack Part No. Pack. x 0 mm 8007 CORTECS UPLC C8+. x 0 mm CORTECS UPLC C8+ CORTECS UPLC C8+ CORTECS UPLC C8+ CORTECS UPLC C8+ CORTECS UPLC C8+ Chemistry Particle Size CORTECS UPLC C8+ CORTECS UPLC C8. x 7 mm CORTECS UPLC C8 CORTECS UPLC C8. x 00 mm CORTECS UPLC C8. x 00 mm CORTECS UPLC C8. x 0 mm CORTECS UPLC C8. x 0 mm CORTECS UPLC C8.0 x 0 mm CORTECS UPLC C8.0 x 0 mm 8007 CORTECS UPLC C8.0 x 0 mm CORTECS UPLC C8.0 x 0 mm 8007 CORTECS UPLC C8.0 x 7 mm CORTECS UPLC C8.0 x 7 mm 8007 CORTECS UPLC C8.0 x 00 mm CORTECS UPLC C8.0 x 00 mm 8007 CORTECS UPLC C8.0 x 0 mm CORTECS UPLC C8.0 x 0 mm x 0 mm x 0 mm x 0 mm x 0 mm x 7 mm x 7 mm x 00 mm x 00 mm x 0 mm x 0 mm x 0 mm x 0 mm x 0 mm x 0 mm x 7 mm x 7 mm x 00 mm x 00 mm x 0 mm x 0 mm *Each kit contains three columns from three batches of material. Description Part No. Particle Size Dimension Part No. CORTECS UPLC C8+. x mm 8007 Reversed-Phase QCRM 800 CORTECS UPLC C8. x mm 8007 Neutrals QCRM x mm 8007 HILIC QCRM 8007 QDa QCRM 8007 LCMS QCRM 8009 For a full list of Waters Analytical Standards and Reagents, visit CORTECS.7 µm Columns Quality Control Reference Materials (QCRM) VanGuard Pre-Columns (Guard Columns) Pack Chemistry

32 SALES OFFICES: Australia Austria Belgium and Luxembourg Brazil 788 Canada China 8 Czech Republic Denmark Finland France Germany Hong Kong Hungary 0 08 India Ireland 8 00 Israel Italy Japan Korea Mexico The Netherlands Norway Poland Portugal Puerto Rico Russia/CIS / Singapore Spain Sweden 8 00 Switzerland Taiwan UAE 97 8 UK US Waters Corporation Maple Street Milford, MA 077 U.S.A. T: F: Waters, The Science of What s Possible, Alliance, CORTECS, ACQUITY, UPLC, and ACQUITY UPLC are registered trademarks of Waters Corporation. CSH is a trademark of Waters Corporation. All other trademarks are property of their respective owners. 0 Waters Corporation. Printed in the U.S.A. February EN Rev C SC-SIG