Table of Contents. Evaluation of Southern Regional MGVI, MGVII and MGVIII Soybean Lines in Cooperation with Dr. Jim Heitholt...16

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2 Tble of Contents Avoiding Insect Problems on the Upper Gulf Cost by Plnting Mturity Group V nd VI Soybens...1 Evlution of Southern Regionl MGVI, MGVII nd MGVIII Soyben Lines in Coopertion with Dr. Jim Heitholt...16 Evlution of Syngent Advnced Soyben Lines in Coopertion with Dr. Glenn Bowers...22 Determining the Influence of Seed Tretments of Cruiser 5FS nd Folir Applictions of Qudris on Soyben Yield...26 MGIV Soyben Insecticide Screening Experiment...30 MGVII Soyben Insecticide Screening Experiment...35 Soyben Performnce Tril t Beumont, TX...41 Evluting Sugrcne Vrieties in Southest Texs. Beumont, TX Mexicn Rice Borer Detected Est of Hrris County in Informtion presented in this report does not constitute n endorsement of product(s) by the Texs Agriculturl Experiment Sttion or the Texs Coopertive Extension. i

3 Avoiding Soyben Insect Problems on the Upper Gulf Cost by Plnting Mturity Group V nd VI Soybens Project investigtors: Dr. M.O. Wy nd M.S. Nunez Technicin: R.G. Wllce Grdute student: R.A. Wolff Student ssistnts: M.S. Weiss nd J.M. Lee Administrtive support: C.D. Tribble Introduction Mturity Group (MG) IV soybens re normlly plnted erly (Apr 1-15) on the Upper Gulf Cost of Texs in order to void potentil drought conditions during lte summer nd lso fcilitte timely hrvest. However, MGIV soybens re usully severely ttcked by stink bugs [southern green stink bug (SGSB), Nezr viridul; green stink bug (GSB), Acrosternum hilre; nd brown sting bug (BSB), Euschistus servus] during the criticl pod-fill stge. Lte MG soybens (VIIs nd VIIIs) re normlly plnted mid-my to mid-june nd mture much lter thn MGIV soybens. These lter mturing soybens cn suffer from drought conditions during pod-fill nd typiclly re exposed to inclement wether s hrvest ners resulting in seed deteriortion nd hrvesting problems. MGVII nd VIII soybens re often ttcked by dmging popultions of defoliting Lepidopter [velvetben cterpillr (VBC), Anticrsi gemmtlis; green cloverworm (GCW), Plthypen scbr; nd soyben looper (SL), Pseudoplusi includens]. MGV nd/or MGVI soybens, however, plnted on selected dtes my void both severe stink bug nd Lepidopter pressure nd mture under more fvorble hrvest conditions. The objective of this study ws to monitor insect popultions nd dmge nd compre yield nd seed qulity mong MGIV, V, VI, nd VII soybens plnted cross three dtes. Mterils nd Methods The study consisted of three plnting dtes on Morey silt lom t the TAMU Agriculturl Reserch nd Extension Center t Beumont in RA 452 (MGIV) ws plnted on 17 Apr. Beds were cultivted prior to plnting nd First 0.75 oz/a nd Dul II 2.5 pt/a were pplied preemergence (PRE) for erly seson weed control with 4-row trctor-mounted spry tnk nd boom (35 gp). S50-N3 (MGV), NC ROY (MGVI), nd DP 7220RR (MGVII) were plnted on 21 My. Beds were cultivted prior to plnting but received no PRE herbicide. 1.5 qt/a ws lter post-directed on the DP 7220RR plots on 14 Jun with hnd-held 1-nozzle spry boom (15 gp). S50-N3 nd NC ROY plots were hnd-hoed s needed erly in the seson nd lter post-directed with 1.5 pt/a, Blzer 1.0 pt/a, 1.5 pt/a, nd Agri- 1.0 pt/a on 16 Jul with the 1-nozzle hnd-held spry boom (15 gp). S50-N3, NC ROY, nd DP 7220RR were gin plnted on 6 Jul. Beds were cultivted prior to plnting nd First 0.75 oz/a nd Dul II 2.5 pt/a were pplied PRE for erly seson weed control with the trctor-mounted tnk nd boom (35 gp). The second plnting dte (My 21) ws cultivted on 5 Jul. The Apr 17 nd Jul 6 plnting dtes received no post-emergence cultivtion. Plot size for ll 1

4 three plnting dtes consisted of 8 rows, 30 inch row width, trimmed to 43 ft (0.02 cres). Plnting dtes were rndomized on the field nd four replictions of treted (T) nd untreted (U) plot for ech vriety pired within its respective plnting dtes (Tble 1). All plnting dtes were scouted for insects s the seson progressed. As popultions of stink bugs nd/or Lepidopter ppered in plots, smpling ws begun. Smpling consisted of 20 sweeps with cnvs net down the entire length (43 ft) of one row in ech plot. Contents of the net were trnsferred to plstic bg, frozen nd lter ll insects identified nd counted in the lb. These bseline counts were begun on 13 Jul. Immeditely following, Orthene 90S ws 1.0 lb (AI)/A to ll treted plots with two-person hnd-held spry boom ( nozzles, 50 mesh screens, 20 ft spry swth, 20 gp). Subsequent 20 sweeps per plot were conducted in n djcent row pproximtely every 10 dys for the reminder of the growing seson (Tble 2). Orthene 90S gin ws 1.0 lb (AI)/A t pproximtely 10 dy intervls to ll treted plots to ensure control of both stink bugs nd Lepidopter throughout the growing seson (Tble 2). Untreted plots received no insecticidl control. Vegettive nd reproductive growth stges of ech cultivr nd within ech plnting dte were recorded on the dtes 20 sweeps were collected (Tble 2). At individul plot mturity, plnt heights were mesured nd the 4 middle rows of ech 8-row plot (43 ft long, 0.01 cres) were hrvested with n Almco SPC20 plot combine. Yields were determined nd djusted to 13% moisture nd 60 lb/bu. A visul estimte of seed qulity ws lso ssessed (1 = excellent, 5 = very poor). All dt were nlyzed s split plot using ANOVA nd LSD (min plot = vriety/plnting dte, subplot = treted or untreted for insects). Insect dt were trnsformed using x 05. before nlysis. Results Southern green stink bug (SGSB) ws the predominnt pest species in the experiment. Lepidopter lrve (VBC, GCW nd SL) were lso identified nd counted but never present in dmging numbers. Popultions peked in erly September t or below threshold nd declined. Threecornered lflf hopper, grsshoppers, cucumber beetles, lefhoppers nd predtors (spiders nd ssssin bugs) were lso counted but not present in significnt numbers or ny meningful pttern mong tretments. These dt nd Lepidopter dt re not presented in the tbles. Popultions of stink bugs (nymphs nd dults) begn to increse in RA 452 untreted plots in mid- July when soybens were R4/5 (Tbles 2, 4A, 5A, 6A nd 7A). RA 452 (plnted Apr 17) reched R4 (full pod) bout 1 month erlier thn NC ROY (MGVI) plnted on 21 My. Previous reserch by the Project Investigtors corroborte this observtion - stink bugs begin infesting erly plnted MGIV soybens during pod-fill. Plnting MGIV soybens in erly to mid-april represents the Erly Soyben Production System (ESPS) which is prcticed on the Texs Upper Gulf Cost to void drought conditions during pod-fill nd llow for timely hrvest before the onset of inclement wether. Resons for the timing of this stink bug infesttion re unknown, but the following re possibilities: 1) stink bugs build up popultions on other hosts nd spill over into ESPS soybens during pod-fill; 2) stink bugs rpidly build up popultions in ESPS soybens prior to pod-fill nd ttck in high numbers during pod-fill; 3) ESPS soybens relese voltile substnce during pod-fill which ttrcts dditionl stink bugs; 4) once initil stink bug colonizers estblish in ESPS soybens, these insects relese n ggregtion pheromone which ttrcts other stink bugs; 5) stink 2

5 bug popultion dynmics coincide with ESPS soybens during pod-fill. At ny rte, stink bug nymphs nd dults rpidly incresed to bove-threshold levels (threshold = 7 stink bugs per 20 sweeps) in RA 452 (ESPS) untreted plots from mid-july to mid-aug when soybens were in podfill (Tbles 2, 4A, 5A, 6A nd 7A). A similr phenomenon ws observed for S50-N3 (MGV) soybens plnted My 21 (Tbles 4A, 5A, 6A nd 7A). However, in this cse, stink bugs (primrily SGSB) reched bove-threshold densities lter (erly Aug), peked during mid-aug nd rpidly declined therefter. These dtes lso corresponded to pod-fill for the MGV cultivr (Tble 2). In comprison, NC ROY plnted My 21 ws not exposed to high popultions of stink bugs; in fct, popultions of stink bugs were well below threshold for the entire seson (Tbles 4A, 5A, 6A nd 7A). Anlyses of the stink bug dt show for ll smpling dtes, stink bug popultions were low nd not sttisticlly different between treted nd untreted plots of NC ROY plnted My 21 (Tbles 4B, 5B, 6B nd 7B). On the other hnd, RA 452 untreted plots were so dversely ffected by high stink bug popultions, they were not ble to be hrvested until 16 dys lter thn treted RA 452 plots (Tble 3A). Conversely, there ws little to no difference in hrvest mturity between the treted nd untreted NC ROY plots (My 21 plnting) nd, in fct, were ble to be hrvested on the sme dy. Across treted nd untreted plots, RA 452 mtured on 9 Sep. The MGV cultivr, NC ROY nd the MGVII cultivr plnted My 21 mtured Sep 28, Oct 14 nd Oct 19, respectively (Tble 3B). Thus, RA 452 nd the My 21 plntings ll voided inclement wether during hrvest. Across min plots, NC ROY plnted My 21 yielded more thn ny other cultivr x plnting dte tretment (Tble 3B). Although RA 452 treted plots outyielded NC ROY treted plots (only by 2.2 bu/a), the difference in yield between treted nd untreted plots of RA 452 ws 13.6 bu/a (Tble 3A). The difference in yield between treted nd untreted plots of NC ROY plnted My 21 ws only 3.7 bu/a. These dt show the dmging effect of stink bugs on yield of RA 452. NC ROY did not suffer nerly s much becuse this MGVI cultivr plnted My 21 voided hevy popultions of stink bugs. In ddition, seed qulity ws gretly ffected in RA 452 untreted plots (Tble 3A). For the My 21 plnting of NC ROY, seed qulity ws only slightly less in untreted thn treted plots (Tble 3A). The lte plnting dte (Jul 6) resulted in sttisticlly lower yields for ll three cultivrs when compred to the My 21 plnting (Tble 3B). Seed qulity ws lso slightly poorer except for the MGV cultivr. Hevy infesttions of stink bugs were not present in the Jul 6 plnting, especilly when compred to the erly plnted MGIV cultivr (Tble 7A). Bsed on these dt nd previous reserch, the Project Investigtors believe tht plnting MGVI cultivr in My could void dmging stink bug popultions typiclly ssocited with ESPS soybens grown on the Upper Gulf Cost of Texs. Dt lso show tht MGVI cultivr plnted in My cn produce yields comprble, if not better, thn ESPS soybens or lter MG soybens. In ddition, MGVI soybens plnted in mid-my (trditionlly considered the optimum plnting time for conventionl cultivrs) would llow for erlier hrvest with less risk of wether-relted hrvest difficulties compred to plnting MGVII nd VIII cultivrs commonly grown in counties est of Hrris Co. 3

6 Tble 1. Plot pln for plnting dtes, soyben cultivrs nd treted (T) or untreted (U) designtions for individul plots b. Beumont, TX Plnting dte REP I REP II My 21 NC ROY (T) NC ROY (U) NC ROY (U) NC ROY (T) Apr 17 RA 452 (U) RA 452 (T) RA 452 (T) RA 452 (U) Jul 6 DP 7220RR (U) DP 7220RR (T) DP 7220RR (T) DP 7220RR (U) My 21 S50-N3 (T) S50-N3 (U) S50-N3 (U) S50-N3 (T) Jul 6 NC ROY (T) NC ROY (U) NC ROY (U) NC ROY (T) My 21 DP 7220RR (U) DP 7220RR (T) DP 7220RR (T) DP 7220RR (U) Jul 6 S50-N3 (U) S50-N3 (T) S50-N3 (T) S50-N3 (U) REP III REP IV My 21 S50-N3 (T) S50-N3 (U) S50-N3 (U) S50-N3 (T) Jul 6 DP 7220RR (T) DP 7220RR (U) DP 7220RR (U) DP 7220RR (T) My 21 NC ROY (U) NC ROY (T) NC ROY (T) NC ROY (U) Jul 6 S50-N3 (U) S50-N3 (T) S50-N3 (T) S50-N3 (U) Apr 17 RA 452 (T) RA 452 (U) RA 452 (U) RA 452 (T) Jul 6 NC ROY (T) NC ROY (U) NC ROY (U) NC ROY (T) My 21 DP 7220RR (U) DP 7220RR (T) DP 7220RR (T) DP 7220RR (U) RA 452 = MGIV, S50-N3 = MGV, NC ROY = MGVI, DP 7220RR = MGVII. b Plot size (not to scle) = 8 rows, 30 inch row width, 43 ft long (0.02 cres). 4

7 Tble 2. Insecticidl pplictions nd soyben growth stge t time of insect smpling (20 sweeps/plot) cross four mturity groups (MGIV, V, VI, VII) nd three plnting dtes (Apr 17, My 21, July 6). Beumont, TX April 17 My 21 July 6 Dte MGIV RA 452 MGV S50-N3 MGVI NC ROY MGVII DP 7220RR MGV S50-N3 MGVI NC ROY MGVII DP 7220RR July 13 R4 R2/3 R2 R1 V1 V1 V1 Applied Orthene 90S (April 17 nd My 21 plntings) on July 13 (following pretretment sweeps) Applied Orthene 90S (April 17 nd My 21 plntings) on July 21 July 23 R5 R3 R2/3 R1/2 V3 V3 V3 Applied Orthene 90S (April 17 nd My 21 plntings) on Aug 2 Aug 3 R5/6 R4 R3 R2 V6/7 V6/7 V5 Applied Orthene 90S (April 17 nd My 21 plntings) on Aug 13 (prior to 20 sweeps on Aug 13) Aug 13 R6 R6 R5 R3/4 R2 R2 V8 Applied Orthene 90S (ll plnting dtes) on Aug 22 Aug 23 R6/7 R6 R5 R4 R3 R2 R2 Applied Orthene 90S (My 21 nd July 6 plntings) on Aug 31 Sep b R7 R6 R5 R4 R4 R2 Sep R7 R6 R5/6 R5 R5 R3 Applied Orthene 90S (My 21 nd July 6 plntings) on Sep 16 Sep R6 R6 R6 R6 R5 Applied Orthene 90S (My 21/MGVII only nd July 6 plntings) on Sep 30 Oct R7 --- R6 R6 Oct R6 Insecticidl pplictions consisted of Orthene 90S pplied t 1.0 lb (AI)/cre only on plots designted s treted for insects. b --- indictes plots lredy hrvested or ner mturity nd 20 sweeps were discontinued. 5

8 Tble 3A. Agronomic nd yield dt for the soyben mturity group/plnting dte vs. insects study. Beumont, TX Vriety Mturit y group Plnting dte Tretment Plnt ht. (in.) Mture dte Seed qul. b (1-5) Yield c (bu/a) RA 452 IV Apr 17 T 29 Sep U 30 Sep S50-N3 V My 21 T 33 Sep U 35 Oct Jul 6 T 27 Oct U 26 Oct NC ROY VI My 21 T 26 Oct U 26 Oct Jul 6 T 25 Nov U 23 Nov DP 7220RR VII My 21 T 31 Oct U 32 Oct Jul 6 T 26 Nov U 24 Dec Tretment: T = treted for insects with Orthene 1.0 lb (AI)/A, U = untreted. b Seed qulity: Visul estimte (1 = excellent, 5 = very poor). c Yield (bu/a): Adusted to 13% moisture nd 60 lb/bu. See Tble 3B for sttisticl nlysis of gronomic nd yield dt. 6

9 Tble 3B. Sttisticl nlysis of gronomic nd yield dt from Tble 3A. Beumont, TX Plnt ht. (in.) Mture dte Seed qul. (1-5) Yield (bu/a) Min plot (soyben mturity group/plnting dte) effects: RA 452 (MGIV) plnted Apr 17 29c Sep 9f 3.3b 29.6b S50-N3 (MGV) plnted My Sep 28e 3.0c 26.5bc S50-N3 (MGV) plnted Jul 6 27d Oct 16d 2.6d 18.2d NC ROY (MGVI) plnted My 21 26d Oct 14d 2.5d 32.3 NC ROY (MGVI) plnted Jul 6 24e Nov 6b 2.7d 26.1bc DP 7220RR (MGVII) plnted My 21 31b Oct 19c 3.3b 23.9c DP 7220RR (MGVII) plnted Jul 6 25de Nov d Subplot (treted or untreted for insects) effects: Treted 28 Oct 12b 2.5b 27.7 Untreted 28 Oct b Interction (min plot x subplot): NS SIG SIG NS Seed qulity: Visul estimte (1 = excellent, 5 = very poor). b Yield (bu/a): Adusted to 13% moisture nd 60 lb/bu. Mens in column followed by the sme or no letter re not significntly (NS) different t the 5% level (ANOVA, LSD). 7

10 Tble 4A. Southern green stink bug dult dt for soyben mturity group/plnting dte vs. insects. Beumont, TX no. southern green stink bug dult/20 sweeps Vriety Mturity group Plnting Jul Dte Trt 13 Jul 23 Aug 3 Aug 13 Aug 23 Sep 1 Sep 10 Sep 20 Oct 4 Oct 14 RA 452 IV Apr 17 T H b H H H H U H H H H H S50-N3 V My 21 T H H H U H H H Jul 6 T H H U H H NC ROY VI My 21 T H H U H H Jul 6 T H U H DP 7220RR VII My 21 T H U H Jul 6 T U Tretment: T = treted for insects, U = untreted for insects. b H = plots previously hrvested. See Tble 4B for sttisticl nlysis of southern green stink bug dult dt. 8

11 Tble 4B. Sttisticl nlysis of southern green stink bug dult dt from Tble 4A. Beumont, TX no. southern green stink bug dult/20 sweeps Jul 13 Jul 23 Aug 3 Aug 13 Aug 23 Sep 1 Sep 10 Sep 20 Oct 4 Oct 14 Min plot (soyben mturity group/plnting dte) effects: RA 452 (MGIV/Apr 17) H b H H H H S50-N3 (MGV/My 21) b 1.0b H H H S50-N3 (MGV/Jul 6) H H NC ROY (MGVI/My 21) 0 0c 0c 0.3b H H NC ROY (MGVI/Jul 6) H DP 7220RR (MGVII/My 21) 0 0.4bc 0.1c 0.4b H DP 7220RR (MGVII/Jul 6) Subplot (treted or untreted for insects) effects: Treted b 0.3b 0.6b 0 0.1b 0.1b Untreted Interction (min plot x subplot): NS SIG SIG SIG NS NS NS NS NS NS Mens in column followed by the sme or no letter re not significntly (NS) different t the 5% level (ANOVA, LSD). b H = plots previously hrvested. 9

12 Tble 5A. Southern green stink bug nymph dt for soyben mturity group/plnting dte vs. insects. Beumont, TX no. southern green stink bug nymph/20 sweeps Vriety Mturity group Plnting Jul Dte Trt 13 Jul 23 Aug 3 Aug 13 Aug 23 Sep 1 Sep 10 Sep 20 Oct 4 Oct 14 RA 452 IV Apr 17 T H b H H H H U H H H H H S50-N3 V My 21 T H H H U H H H Jul 6 T H H U H H NC ROY VI My 21 T H H U H H Jul 6 T H U H DP 7220RR VII My 21 T H U H Jul 6 T U Tretment: T = treted for insects, U = untreted for insects. b H = plots previously hrvested. See Tble 5B for sttisticl nlysis of southern green stink bug nymph dt. 10

13 Tble 5B. Sttisticl nlysis of southern green stink bug nymph dt from Tble 5A. Beumont, TX no. southern green stink bug nymph/20 sweeps Jul 13 Jul 23 Aug 3 Aug 13 Aug 23 Sep 1 Sep 10 Sep 20 Oct 4 Oct 14 Min plot (soyben mturity group/plnting dte) effects: RA 452 (MGIV/Apr 17) b H b H H H H S50-N3 (MGV/My 21) b 3.1b H H H S50-N3 (MGV/Jul 6) 0b H H NC ROY (MGVI/My 21) 0 0.4b 0.5c 0b 0.3b H H NC ROY (MGVI/Jul 6) 0b H DP 7220RR (MGVII/My 21) 0 0.9b 0.6c 0.1b 0b b H DP 7220RR (MGVII/Jul 6) 0b b 0.8 Subplot (treted or untreted for insects) effects: Treted b 0.1b 1.0 0b 0b 0.1b 0b Untreted Interction (min plot x subplot): NS SIG SIG SIG SIG NS NS NS NS NS Mens in column followed by the sme or no letter re not significntly (NS) different t the 5% level (ANOVA, LSD). b H = plots previously hrvested. 11

14 Tble 6A. Southern green stink bug (dult + nymph) dt for soyben mturity group/plnting dte vs. insects. Beumont, TX no. southern green stink bug (dult + nymph)/20 sweeps Vriety Mturity group Plnting Jul Dte Trt 13 Jul 23 Aug 3 Aug 13 Aug 23 Sep 1 Sep 10 Sep 20 Oct 4 Oct 14 RA 452 IV Apr 17 T H b H H H H U H H H H H S50-N3 V My 21 T H H H U H H H Jul 6 T H H U H H NC ROY VI My 21 T H H U H H Jul 6 T H U H DP 7220RR VII My 21 T H U H Jul 6 T U Tretment: T = treted for insects, U = untreted for insects. b H = plots previously hrvested. See Tble 6B for sttisticl nlysis of southern green stink bug (dult + nymph) dt. 12

15 Tble 6B. Sttisticl nlysis of southern green stink bug (dult + nymph) dt from Tble 6A. Beumont, TX no. southern green stink bug (dult + nymph)/20 sweeps Jul 13 Jul 23 Aug 3 Aug 13 Aug 23 Sep 1 Sep 10 Sep 20 Oct 4 Oct 14 Min plot (soyben mturity group/plnting dte) effects: RA 452 (MGIV/Apr 17) b H b H H H H S50-N3 (MGV/My 21) b 4.1b b H H H S50-N3 (MGV/Jul 6) 0b H H NC ROY (MGVI/My 21) 0 0.4b 0.5c 0.3b 0.3b H H NC ROY (MGVI/Jul 6) 0b 0.6 0b H DP 7220RR (MGVII/My 21) 0 1.3b 0.8c 0.5b 0.3b b H DP 7220RR (MGVII/Jul 6) 0b b Subplot (treted or untreted for insects) effects: Treted b 0.4b 1.6b 0b 0.1b 0.2b 0b 0.2b 0.8 Untreted Interction (min plot x subplot): NS SIG SIG SIG SIG NS NS NS NS NS Mens in column followed by the sme or no letter re not significntly (NS) different t the 5% level (ANOVA, LSD). b H = plots previously hrvested. 13

16 Tble 7A. Totl phytophgous stink bugs (dult + nymph) dt for soyben mturity group/plnting dte vs. insects. Beumont, TX no. totl phytophgous stink bugs (dult + nymph)/20 sweeps Vriety Mturity group Plnting Jul Dte Trt 13 Jul 23 Aug 3 Aug 13 Aug 23 Sep 1 Sep 10 Sep 20 Oct 4 Oct 14 RA 452 IV Apr 17 T H b H H H H U H H H H H S50-N3 V My 21 T H H H U H H H Jul 6 T H H U H H NC ROY VI My 21 T H H U H H Jul 6 T H U H DP 7220RR VII My 21 T H U H Jul 6 T U Totl phytophgous stink bug = southern green stink bug (SGSB), green stink bug (GSB), nd brown stink bug (BSB). b Tretment: T = treted for insects, U = untreted for insects. See Tble 7B for sttisticl nlysis of totl phytophgous stink bugs (dult + nymph) dt. 14

17 Tble 7B. Sttisticl nlysis of totl phytophgous stink bugs (dult + nymph) dt from Tble 7A. Beumont, TX no. totl phytophgous stink bugs (dult + nymph)/20 sweeps Jul 13 Jul 23 Aug 3 Aug 13 Aug 23 Sep 1 Sep 10 Sep 20 Oct 4 Oct 14 Min plot (soyben mturity group/plnting dte) effects: RA 452 (MGIV/Apr 17) b H b H H H H S50-N3 (MGV/My 21) 0.4b 1.4b 4.8b b H H H S50-N3 (MGV/Jul 6) 0b 0.3bc 1.6b 3.6 H H NC ROY (MGVI/My 21) 0.3b 0.4b 0.8c 0.3b 0.3b 1.5b b H H NC ROY (MGVI/Jul 6) 0b 0.6bc 0.5b 1.4b 3.6 H DP 7220RR (MGVII/My 21) 0b 1.4b 0.8c 0.9b 0.4b 0.1c 1.3b 0.9b 0.4b H DP 7220RR (MGVII/Jul 6) 0.1b 0.5bc 0.5b 1.5b Subplot (treted or untreted for insects) effects: Treted b 0.6b 1.7b 0b 0.1b 0.5b 0.2b 0.7b 1.3 Untreted Interction (min plot x subplot): NS SIG SIG SIG SIG SIG NS SIG SIG NS Mens in column followed by the sme or no letter re not significntly (NS) different t the 5% level (ANOVA, LSD). b H = plots previously hrvested. 15

18 Evlution of Southern Regionl MGVI, MGVII nd MGVIII Soyben Lines in Coopertion with Dr. Jim Heitholt Project investigtor: Technicins: Grdute student: Student ssistnts: Administrtive support: Dr. M.O. Wy M.S. Nunez nd R.G. Wllce R.A. Wolff M.S. Weiss nd J.M. Lee C.D. Tribble Introduction The objective of this reserch ws to ssess yield, seed qulity nd mturity dte on experimentl Mturity Group (MG) VI, MGVII nd MGVIII soyben lines grown on the Texs Upper Gulf Cost. The reserch consisted of 33 Preliminry MGVI entries, 18 Uniform MGVI entries, 24 Preliminry MGVII entries, 15 Uniform MGVII entries, nd 18 Preliminry MGVIII entries. These five tests were prt of lrger scope of Southern Regionl soyben reserch conducted by Dr. Jim Heitholt (Soyben Agronomist, Texs A&M University Agriculturl Reserch nd Extension Center t Dlls). Mterils nd Methods The soyben plntings were conducted on Morey silt lom t the Texs Agriculturl Reserch nd Extension Center t Beumont in Beds for the tests were pulled on 30 Mr (30 in. row width) nd lter cultivted on 16 Apr. The beds were gin cultivted just prior to plnting on 21 My. Plot size ws four rows, 30 inch row width, trimmed to 15 ft long (0.003 cres). The Preliminry tests consisted of two replictions nd the Uniform tests consisted of three replictions, ech test in n individully rndomized complete block. First 0.75 oz/a nd Dul II 2.5 pt/a were pplied preemergence (PRE) on 25 My for erly seson weed control with 4-row trctor-mounted spry tnk nd boom (35 gp). Plots were cultivted once during the growing seson on 5 Jul. On 11 Aug, n eril ppliction of Mustng 4oz/A nd 2 oz/a ws pplied to ll plots for insect control (minly Lepidopter lrve). There were no irrigtions. Lodging ws not observed in ny plots. The two middle rows of ech plot (15 ft long, cres) were hrvested individully t mturity by hnd or with n Almco SPC20 plot combine. Plnt heights lso were mesured t mturity. Yields were determined, djusted to 13% moisture nd 60 lb/bu nd nlyzed using ANOVA nd LSD (Tbles 1-5). Seed qulity lso ws ssessed nd given visul rting (1 = excellent, 5 = very poor). Results The Preliminry nd Uniform MG VI soyben lines generlly yielded higher thn the MGVII nd MGVII lines. Test mens were 32.1 nd 32.6 bu/a, respectively (Tbles 1-2). Highest yield overll ws 48.4 bu/a (Tble 2, Uniform MGVI). The erlier mturing soybens lso experienced noticebly better seed qulity t hrvest. The MGVII soyben lines yielded somewht less thn the MGVI lines. Test mens were 25.2 nd 28.3 bu/a (Tbles 3-4). Seed qulity lso declined in these lter mturing soybens. The Preliminry MGVIII lines yielded lowest with the worst seed qulity (Tble 5). These ltest mturing lines were subjected to inclement wether conditions during pod-fill nd s hrvest pproched. Some MGVIII lines, in prticulr, were not ble to fully mture resulting in green stems nd extremely poor pod condition. Test men for the MGVIII lines ws 19.3 bu/a. 16

19 Tble 1. Evlution of Preliminry MGVI soyben lines t Beumont, TX Seed Strin or Mture Plnt ht. qulity Yield b check vriety dte (inches) (1-5) (bu/a) R Oct NC ROY Oct NCC Oct VS Oct Au Oct VS Oct R Oct NCC Oct Au Oct SC01-778RR Nov BOGGS RR Oct NCC Oct VS Oct N Oct R Oct R Oct NTCPR01-42 Oct Au Oct VS Oct SC01-669RR Oct NCC RR Oct G03-614RR Nov NCC Oct VS Oct Au Oct DILLON Oct VS Oct R F Oct SC01-698RR Nov Derry Oct SC Oct Rndolph Oct Asmr Oct Test men = 32.1 bu/a CV = 19.9% LSD(0.05) = 13.0 bu/a Seed qulity: Visul estimte (1 = excellent, 5 = very poor). b Yields in bold itlics re not sttisticlly different thn the highest yielding entry t the 5% level (ANOVA, LSD). 17

20 Tble 2. Evlution of Uniform MGVI soyben lines t Beumont, TX Seed Strin or Mture Plnt ht. qulity Yield b check vriety dte (inches) (1-5) (bu/a) R Oct NC ROY Oct BOGGS RR Oct R Oct R Oct R Oct R Oct VS Oct VS Oct VS Nov SC Nov NTCPPR Oct DILLON Oct Au Oct VS Oct VS Oct Au Oct VS Oct Test men = 32.6 bu/a CV = 14.5% LSD(0.05) = 7.9 bu/a Seed qulity: Visul estimte (1 = excellent, 5 = very poor). b Yields in bold itlics re not sttisticlly different thn the highest yielding entry t the 5% level (ANOVA, LSD). 18

21 Tble 3. Evlution of Preliminry MGVII soyben lines t Beumont, TX Seed Strin or Mture Plnt ht. qulity Yield b check vriety dte (inches) (1-5) (bu/a) N Nov G03-830RR Oct BENNING Nov N Nov N Nov Au Nov SC01-796RR Nov Au Nov Au Nov N Oct SC01-786RR Nov G03-940RR Dec HASKELL RR Nov Au Nov G03-557RR Nov SC01-779RR Nov G03-926RR Dec SC01-784RR Oct SC01-783RR Nov SC01-819RR Dec G03-364RR Dec Tyrone Oct G03-630RR Dec G03-332RR Dec Test men = 25.2 bu/a CV = 20.0% LSD(0.05) = 10.4 bu/a Seed qulity: Visul estimte (1 = excellent, 5 = very poor). b Yields in bold itlics re not sttisticlly different thn the highest yielding entry t the 5% level (ANOVA, LSD). 19

22 Tble 4. Evlution of Uniform MGVII soyben lines t Beumont, TX Seed Strin or Mture Plnt ht. qulity Yield b check vriety dte (inches) (1-5) (bu/a) G Nov SC Nov N Nov G Nov N Nov G Nov BENNING Nov G Nov N Nov N Dec HASKELL RR Dec G03-G1126RR Nov SC Nov G Dec SC00-601RR Dec Test men = 28.3 bu/a CV = 18.6% LSD(0.05) = 8.9 bu/a Seed qulity: Visul estimte (1 = excellent, 5 = very poor). b Yields in bold itlics re not sttisticlly different thn the highest yielding entry t the 5% level (ANOVA, LSD). 20

23 Tble 5. Evlution of Preliminry MGVIII soyben lines t Beumont, TX Seed Strin or Mture Plnt ht. qulity Yield b check vriety dte (inches) (1-5) (bu/a) SC Nov SC01-798RR Dec COOK Dec SC01-794RR Dec SC01-832RR Dec NTC02AXB-717 Nov G03-548RR Dec SC01-793RR Dec G03-394RR Nov SC01-809RR Dec SC01-803RR Dec SC01-805RR Dec G03-533RR Dec G03-695RR Dec PRICHARD RR Dec G03-425RR Dec G03-952RR Dec G03-893RR Dec Test men = 19.3 bu/a CV = 36.9% LSD(0.05) = 15.0 bu/a Seed qulity: Visul estimte (1 = excellent, 5 = very poor). b Yields in bold itlics re not sttisticlly different thn the highest yielding entry t the 5% level (ANOVA, LSD). 21

24 Evlution of Syngent Advnced Soyben Lines in Coopertion with Dr. Glenn Bowers Project investigtor: Technicins: Grdute student: Student ssistnts: Administrtive support: Dr. M.O. Wy M.S. Nunez nd R.G. Wllce R.A. Wolff M.S. Weiss nd J.M. Lee C.D. Tribble Introduction The objective of this reserch ws to ssess yield, seed qulity nd mturity dte on experimentl soyben lines grown on the Texs Upper Gulf Cost. Soyben entries were provided by Dr. Glenn Bowers (Soyben Physiologist, Syngent). The reserch consisted of Advnced Test 560 (36 entries), Advnced Test 567 (25 entries) nd Advnced Test 570 (36 entries). Mterils nd Methods The soyben plntings were conducted on Morey silt lom t the Texs Agriculturl Reserch nd Extension Center t Beumont in Beds for the tests were pulled on 30 Mr (30 inch row width) nd lter cultivted on 16 Apr. The beds were gin cultivted just prior to plnting on 21 My. Plot size ws four rows, 30 inch row width, trimmed to 15 ft long (0.003 cres). All tests consisted of two replictions, ech test in n individully rndomized complete block. First 0.75 oz/a nd Dul II 2.5 pt/a were pplied preemergence (PRE) on 25 My for erly seson weed control with 4-row trctor-mounted spry tnk nd boom (35 gp). Plots were cultivted once during the growing seson on 5 Jul. On 11 Aug, n eril ppliction of Mustng 4oz/A nd 2 oz/a ws pplied to ll plots for insect control (minly Lepidopter lrve). There were no irrigtions. Lodging ws not observed in ny plots. The two middle rows of ech plot (15 ft long, cres) were hrvested t mturity by hnd or with n Almco SPC20 plot combine. Plnt heights lso were mesured t mturity. Yields were determined, djusted to 13% moisture nd 60 lb/bu nd nlyzed using ANOVA nd LSD (Tbles 1-3). Seed qulity lso ws ssessed nd given visul rting (1 = excellent, 5 = very poor). Results The erlier mturing entries (Tble 1, Advnced Test 560) tended to yield higher with better seed qulity prtly due to n erlier hrvest under more fvorble environmentl conditions. Seed qulity in lter mturing entries suffered from deteriorting wether conditions during lte pod-fill nd pproching hrvest (Tbles 2-3, Advnced Tests 567 nd 570). Drought conditions existed during t lest portion of the reproductive stge for ll three tests. Some entries my hve been ffected more thn others. Yields rnged from 43.8 bu/a (Advnced Test 567) to 8.3 bu/a (Advnced Test 570). Soyben lines from Advnced Test 560 (Mturity Groups ) performed best overll with test men of 32.8 bu/a (Tble 1). Mny soyben lines were not dptble to the Upper Gulf Cost environment. Although they hd cceptble yields, they often suffered from poor seed qulity t hrvest. Other lines, however, yielded well with cceptble seed qulity. 22

25 Tble 1. Evlution of Syngent Advnced Test 560 soyben lines (MG ) t Beumont, TX. Seed Strin or Mture Plnt ht. qulity Yield b check vriety dte (inches) (1-5) (bu/a) S59-V6 Sep BA Oct P96-B21 Oct BA Oct BA Oct KG Sep AG6202 Oct KG Oct P95B96 Oct KG Oct KG Oct BA Oct KG Oct KG Oct KG Oct BA04694 Oct KG Oct AG5901 Oct S64-J1 Oct KG Sep KG Oct KG Oct KG Oct KG Oct KG Sep KG Oct S57-P1 Oct BA Oct KG Oct KG Oct KG Oct BA Oct KG Oct KG Oct KG Oct KG Oct Test men = 32.8 bu/a CV = 12.5% LSD(0.05) = 8.3 bu/a Seed qulity: Visul estimte (1 = excellent, 5 = very poor). b Yields in bold itlics re not sttisticlly different thn the highest yielding entry t the 5% level (ANOVA, LSD). 23

26 Tble 2. Evlution of Syngent Advnced Test 567 soyben lines (MG ) t Beumont, TX. Seed Strin or Mture Plnt ht. qulity Yield b check vriety dte (inches) (1-5) (bu/a) BA Oct BA Oct BA Oct P95B97 Oct BA Oct BA Oct HBK5992 Oct BA Oct Benning Nov BA Oct BA Oct BA Oct BA Oct Prichrd Nov BA Oct BA Oct BA Dec BA Nov BA Nov BA Dec BA Nov BA Dec BA Nov BA Dec BA Dec Test men = 30.3 bu/a CV = 18.3% LSD(0.05) = 11.5 bu/a Seed qulity: Visul estimte (1 = excellent, 5 = very poor). b Yields in bold itlics re not sttisticlly different thn the highest yielding entry t the 5% level (ANOVA, LSD). 24

27 Tble 3. Evlution of Syngent Advnced Test 570 soyben lines (MG ) t Beumont, TX. Seed Strin or Mture Plnt ht. qulity Yield b check vriety dte (inches) (1-5) (bu/a) DP6880RR Oct H6255RR Oct KG Nov DP7220RR Oct KG Oct AG7601 Nov KG Oct KG Oct S76-L9 Nov KG Oct BA Nov BA Nov KG Oct KG Nov KG Oct KG Oct KG Oct KG Dec BA Nov KG Nov KG Nov KG Nov BA Dec BA Nov KG Dec KG Nov KG Oct KG Dec KG Dec KG Dec KG Dec BA Dec KG Dec S73-Z5 Dec KG Dec BA Dec Test men = 25.2 bu/a CV = 22.7% LSD(0.05) = 11.8 bu/a Seed qulity: Visul estimte (1 = excellent, 5 = very poor). b Yields in bold itlics re not sttisticlly different thn the highest yielding entry t the 5% level (ANOVA, LSD). 25

28 Determining the Influence of Seed Tretments of Cruiser 5FS nd Folir Applictions of Qudris on Soyben Yield Beumont, TX 2004 Syngent CHRONOLOGICAL INFORMATION nd PRODUCTION PRACTICES Mr 31 Seed treted by SYNGENTA (see Tbles for tretment descriptions, rtes nd timings) My 21 Cultivted beds prior to plnting (Morey silt lom) My 22 Plnted 222 seeds per 25 ft of row (Vriety = S73-Z5, MGVII) Plot size = 4 rows, 30 inch row width, 25 ft long, cres, 4 replictions My 24 Applied First 0.75 oz/a nd Dul II 2.5 pt/a preemergence (PRE) with 4-row trctor-mounted spry tnk nd boom (35 gp) for erly seson weed control June 1 Counted dult nd nymph thrips on 20 plnts/plot Estimted defolition (%) on 20 plnts/plot June 7 Counted number of plnts in 3 ft section of the 2 middle rows of ech plot (stnd count) June 22 Counted threecornered lflf hopper (TCAH) girdles on min stem nd petioles of 5 plnts/plot Estimted defolition (%) on 5 plnts/plot Mesured plnt height (inches) nd vegettive growth stge on 5 plnts/plot July 5 July 29 Aug 16 Aug 28 Dec 15 Cultivted rows Applied folir ppliction tretments of 6.0 oz/cre with 2-row hnd-held spry boom (8002 cone nozzles, 50 mesh screens, 12 gp spry volume) Soybens t growth stge R3 t time of ppliction Plots evluted by Tom Iskeit for southern blight, lef spot, web blight Only trce mounts of diseses noted (ll < 1% of lef re or plnt ffected) Treted ll plots with Krte 0.03 lb (AI)/cre for lte seson Lepidopter control with 2-row hnd-held spry boom (8002 cone nozzles, 50 mesh screens, 12 gp spry volume) Hrvested plots with n Almco SPC20 plot combine (Size hrvested plot = 2 middle rows, 30 inch row width, 25 ft long, cres) Yields determined nd djusted to 13% moisture nd 60 lb/bu Visul estimte of seed qulity ssessed (1 = excellent, 5 = very poor) Thrip nd TCAH girdle dt were trnsformed using before nlysis. x 05. Defolition dt were subjected to rcsine trnsformtion before nlysis. All dt were nlyzed using ANOVA nd LSD t the 5% level. 26

29 Determining the Influence of Seed Tretments of Cruiser 5FS nd Folir Applictions of Qudris on Soyben Yield Discussion A significnt difference mong tretments occurred erly in the seson with respect to thrip counts (thrips were identified s Frnkliniell occidentlis). Thrips were not found in plots seed-treted with Cruiser 5FS (Tble 1). Plots were inspected for threecornered lflf hopper girdles but reveled no dmge (Tble 2). Otherwise, plots cross ll tretments developed similrly in the erly growing seson nd there were no significnt differences in yield or seed qulity t hrvest (Tble 3). The vriety S73-Z5 proved to be not very dptble to the Upper Gulf Cost environmentl conditions s evidenced by extremely poor seed qulity in ll plots. No significnt conclusions cn be drwn s to the effectiveness of the Cruiser 5FS seed tretments nd/or folir pplictions of Qudris in incresing soyben yield. Tble 1. SYNGENTA soyben seed tretment test [thrips (Frnkliniell occidentlis) nd defolition dt on June 1]. Beumont,TX Rte No. thrips/plnt b Defolition c Tretment g (AI)/100 kg seed Nymphs Adults Totl (%) Untreted c 3 b 7 c 2 Fungicide only Fungicide + Cruiser 5FS c 3 b 7 bc Fungicide + Qudris Fungicide + Cruiser 5FS + Qudris fl oz/c fl oz/a 2 b 3 b 5 b NS Tretment: Fungicide (APRON MAXX RTA + MOLY ES) nd Cruiser 5FS pplied s seed tretments; Qudris pplied s folir spry (6.0 fl oz/a) t R3 growth stge. b Derived from the men of 20 plnts/plot. c Visul estimte of defolition derived from the men of 20 plnts/plot. Mens in column followed by the sme or no letter re not significntly (NS) different t the 5% level (ANOVA, LSD). 27

30 Tble 2. SYNGENTA soyben seed tretment test (plnt chrcteristics, threecornered lflf hopper, nd defolition dt on June 22). Beumont, TX Rte Threecornered lflf hopper girdles b Growth Plnt ht. Tretment g (AI)/100 kg seed stge (inches) Min stem Petioles Totl Defol. c (%) Untreted --- V Fungicide only Fungicide + Cruiser 5FS 6.25 V V Fungicide + Qudris Fungicide + Cruiser 5FS + Qudris fl oz/c fl oz/a V V NS NS NS NS NS NS Tretment: Fungicide (APRON MAXX RTA + MOLY ES) nd Cruiser 5FS pplied s seed tretments; Qudris pplied s folir spry (6.0 fl oz/a) t R3 growth stge. b Derived from the men of 5 plnts/plot. c Visul estimte of defolition derived from the men of 5 plnts/plot. Mens in ll columns re not significntly (NS) different t the 5% level (ANOVA, LSD). 28

31 Tble 3. SYNGENTA soyben seed tretment test (plnt stnd, yield nd seed qulity). Beumont, TX Tretment Rte Yield lb (AI)/100 kg seed Plnts/ft of row b (bu/cre) c Seed qulity (1-5) d Untreted c Fungicide only Fungicide + Cruiser 5FS b b Fungicide + Qudris Fungicide + Cruiser 5FS + Qudris fl oz/c fl oz/a 7 bc NS NS Tretment: Fungicide (APRON MAXX RTA + MOLY ES) nd Cruiser 5FS pplied s seed tretments; Qudris pplied s folir spry (6.0 fl oz/a) t R3 growth stge. b Derived by counting number of plnts in 3 ft. section of ech of the two middle rows/plot. c Yields djusted to 13% moisture nd 60 lb/bu. d Visul estimte of seed qulity (1 = excellent, 5 = very poor). Mens in column followed by the sme or no letter re not significntly (NS) different t the 5% level (ANOVA, LSD). 29

32 CHRONOLOGICAL INFORMATION MGIV Soyben Insecticide Screening Experiment Texs Agriculturl Experiment Sttion Beumont, TX 2004 Mr 30 Pulled beds for experiment (Morey silt lom) Apr 16 Cultivted beds (30 inch row width) Apr 17 Plnted RA 452 (MGIV soyben) t rte of 8 vible seed per foot of row Plot size lter trimmed to 8 rows, 30 inch row width, 40 ft long, 4 replictions (0.018 cres) Apr 21 Applied First 0.75 oz/a nd Dul II 2.5 pt/a preemergence (PRE) with 2-row hnd-held spry boom (15 gp) Jul 28 Soybens t R4 growth stge. Stink bug popultions noted s on the increse s well s some soyben loopers Aug 3 Pretretment smples re collected (20 sweeps/plot down entire length of one row). Soybens t growth stge R5. Smples were plced in plstic bgs, frozen nd insects lter identified nd counted. Subsequent 20 sweep smples were collected on n djcent row to previous smpling. Aug 4 Mustng Mx t three rtes nd Krte Z t one rte (Tbles 1-6) were pplied to selected plots with 2-person hnd-held spry boom pressurized with CO 2 t 25 psi ( nozzles, 50 mesh screens, 20 ft spry swth, 20 gp spry volume). Aug 6 20 sweeps/plot [2 dys fter tretment (DAT)]. Soybens t R5. Aug 9 20 sweeps/plot (5 DAT). Soybens t R5/6. Aug sweeps/plot (9 DAT). Soybens t R6. Aug sweeps/plot (12 DAT). Soybens t R6. Aug sweeps/plot (16 DAT). Soybens t R6. Aug 25 Finl 20 sweeps/plot (21 DAT). Soybens t R7. Sep 9 Hrvested ll plots except untreted which were still green-stemmed nd not fully mture Size hrvested plot = 4 middle rows, 30 in row width, 40 ft long (0.009 cres) Sep 17 Hrvested untreted plots Note: Yields were determined nd djusted to 13% moisture nd 60 lb/bu. Seed qulity ws ssessed nd given visul rting (1 = excellent, 5 = very poor). Insect counts were trnsformed using x 05. nd ll dt nlyzed using ANOVA nd LSD (Tbles 1-6). 30

33 MGIV Soyben Insecticide Screening Experiment Beumont, TX 2004 Discussion Southern green stink bug (SGSB), Nezr viridul, ws the predominnt insect pest in this experiment. All tretments performed eqully well in controlling this species (Tbles 1-3). The low rte of Mustng Mx ws slightly less effective thn the mid nd high rte t controlling ll species of stink bugs [SGSB, green stink bug (Acrosternum hilre) nd brown stink bug (Euschistus servus)] (Tble 4). Lepidopter lrve (velvetben cterpillr, green cloverworm nd soyben looper) nd threecornered lflf hopper (TCAH) were not significnt pests during pod-fill nd pproching mturity. TCAH dt re not presented due to their presence in extremely low numbers. Lepidopter lrve were on the increse t 5 DAT (Tble 5) but not in significnt numbers to jeoprdize yield. Mustng Mx nd Krte Z ppered to hve little or no residul effect on controlling Lepidopter lrve in this experiment. Although Mustng Mx (ll rtes) nd Krte Z tretments outyielded untreted plots, there ws not sttisticl difference. Yields in insecticide treted plots rnged from 27.6 to 23.7 bu/a compred to 20.2 bu/a in untreted plots. However, ll plots with insecticide tretments (low, mid nd high rte of Mustng Mx, nd Krte Z) benefitted with significntly better seed qulity t hrvest thn untreted plots (Tble 6). Tble 1. Southern green stink bug nymph counts in the insecticide screening test with MGIV soybens. Beumont, TX Tretment Rte lb(ai)/cre No./20 sweeps PRE 2 DAT b 5 DAT 9 DAT 12 DAT 16 DAT 21 DAT Mustng Mx b 1 b Mustng Mx b 0 b Mustng Mx b 1 b Krte Z b 0 b Untreted NS NS NS NS NS PRE = pretretment. b DAT = dys fter tretment. Mens in column followed by the sme or no letter re not sttisticlly (NS) different t the 5% level (ANOVA, LSD). 31

34 Tble 2. Southern green stink bug dult counts in the insecticide screening test with MGIV soybens. Beumont, TX Tretment Rte lb(ai)/cre No./20 sweeps PRE 2 DAT b 5 DAT 9 DAT 12 DAT 16 DAT 21 DAT Mustng Mx b 1 b 1 b 0 b 1 0 Mustng Mx b 0 b 0 b 0 b 1 0 Mustng Mx b 1 b 1 b 1 b 0 0 Krte Z b 1 b 0 b 0 b 0 0 Untreted NS NS NS PRE = pretretment. b DAT = dys fter tretment. Mens in column followed by the sme or no letter re not sttisticlly (NS) different t the 5% level (ANOVA, LSD). Tble 3. Southern green stink bug (nymph nd dult) counts in the insecticide screening test with MGIV soybens. Beumont, TX Tretment Rte lb(ai)/cre No./20 sweeps PRE 2 DAT b 5 DAT 9 DAT 12 DAT 16 DAT 21 DAT Mustng Mx b 2 b 1 b 0 b 1 b 0 Mustng Mx b 1 b 0 b 0 b 1 b 0 Mustng Mx b 1 b 1 b 1 b 0 b 0 Krte Z b 1 b 0 b 0 b 0 b 0 Untreted NS NS PRE = pretretment. b DAT = dys fter tretment. Mens in column followed by the sme or no letter re not sttisticlly (NS) different t the 5% level (ANOVA, LSD). 32

35 Tble 4. Totl phytophgous stink bug nymph nd dult (southern green stink bug, Piezodorus guildinii, brown stink bug) counts in the insecticide screening test with MGIV soybens. Beumont, TX Tretment Rte lb(ai)/cre No./20 sweeps PRE 2 DAT b 5 DAT 9 DAT 12 DAT 16 DAT 21 DAT Mustng Mx b 6 b 1 b Mustng Mx b 1 c 0 b Mustng Mx b 2 c 1 b Krte Z b 4 bc 1 b Untreted NS NS NS NS PRE = pretretment. b DAT = dys fter tretment. Mens in column followed by the sme or no letter re not sttisticlly (NS) different t the 5% level (ANOVA, LSD). Tble 5. Totl Lepidopter lrve (soyben looper, green cloverworm, velvetben cterpillr) counts in the insecticide screening test with MGIV soybens. Beumont, TX Tretment Rte lb(ai)/cre No./20 sweeps PRE 2 DAT b 5 DAT 9 DAT 12 DAT 16 DAT 21 DAT Mustng Mx Mustng Mx Mustng Mx Krte Z Untreted NS NS NS NS NS NS NS PRE = pretretment. b DAT = dys fter tretment. Mens in column followed by the sme or no letter re not sttisticlly (NS) different t the 5% level (ANOVA, LSD). 33

36 Tble 6. Men yield nd seed qulity in MGIV soyben insecticide screening test. Beumont, TX Tretment Rte lb (AI)/cre Yield (bu/cre) Seed qulity (1-5) b Mustng Mx Mustng Mx Mustng Mx Krte Z Untreted b Yield (bu/cre): Adjusted to 13% moisture nd 60 lb/bu. b Seed qulity (1-5): visul estimte, 1 = excellent, 5 = very poor. Mens in column followed by the sme or no letter re not sttisticlly (NS) different t the 5% level (ANOVA, LSD). NS 34

37 MGVII Soyben Insecticide Screening Experiment Texs Agriculturl Experiment Sttion Beumont, Texs 2004 My 22 My 24 June 18 Aug 27 Plnted Pioneer 97B52 RR (MGVII) on Levc soil t vible seed per foot of row Plot size = 8 rows, 40 ft long, 30 inch row width, 4 replictions (0.018 cres) Applied First 0.75 oz/cre nd Dul II 2.5 pt/cre preemergence (PRE) with 4-row trctor-mounted spry tnk nd boom (35 gp) for erly seson weed control Applied Honcho 1.0 qt/cre by irplne for erly-mid seson weed control Note: Cultivtion ws not required for durtion of seson. Pretretment smples collected (20 sweeps/plot with cnvs net down entire length of one row in ech plot). Subsequent 20 sweeps were collected in the row djcent to previous smpling. Smples were plced in plstic bg, frozen nd insects lter identified nd counted. Tretments were rrnged in rndomized complete block. Immeditely following collection of pretretment smples, insecticide tretments were pplied using 2-person hnd-held spry boom pressurized with CO 2 t 25 psi ( nozzles, 50 mesh screens, 20 ft spry swth, 20 gp spry volume). Soybens were t R5 growth stge. Aug sweeps/plot [3 dys fter tretment (DAT )] Soybens t R5 growth stge Sep 6 Sep 13 Oct 15 Dec 4 Note: 20 sweeps/plot (10 DAT) Soybens t R5/R6 growth stge Finl 20 sweeps/plot (17 DAT) Soybens t R6 growth stge Soybens t lte R6 growth stge, leves yellowing, beginning of lef drop Due to dverse wether nd poor hrvesting conditions, plots were hnd-hrvested, crried out of the field, nd lter threshed with n Almco SPC20 plot combine. Size hrvested plot = 1 row, 30 inch row width, 25 ft long ( cres) Yields were determined nd djusted to 13% moisture nd 60 lb/bu. Seed qulity ws ssessed nd given visul rting (1 = excellent, 5 = very poor). Insect counts were trnsformed using x 05. nd ll dt nlyzed using ANOVA nd LSD (Tbles 1-4). 35