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1 Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , 2017 o: /JHD Journl o Hrl Dr u ournl omp: Prprton n pysoml nlyss o trtonl vr s on D. Sop Frnoos Hssnpour 1, Momm Hotolslmy *2, Momm Fzl N 1 1 Dprtmnt o Foo Sn n Tnoloy, Islm Az Unvrsty, Korsn rn, Isn, Irn; 2 Dprtmnt o Foo Sn n Tnoloy, Islm Az Unvrsty, Sror rn, Sror, Irn; *Eml: moot@ml.om ARTICLE INFO ABSTRACT Typ: Ornl Rsr Top: Mnl Plnts Rv July 24 t 2016 Apt Dmr 16 t 2016 Ky wors: D. sop vr stlzton Pysoml Mrol nlyss 1. Introuton D. sop, s n nnul or nnl r n t Crurmly n mmr o t Brss mly w os y snt nms Dsurnsop L. n Ssymrumsop L. Its vr s on o t Irnn trtonl vrs w v mny uss n trtonl mn (Al-Jr, 2011). T most mportnt ompoun o t D. sop Broun & Am: T mn m o ts stuy ws stlzn D. Sop vr n ru ts smntton rt. Exprmntl: Xntn n Croxy mtyl llulos ums v n us n tr lvls (0.05,.1 n.2 r/lt) n mxtur o Gur-xntn n roxymtyl llulos (.1 /l) n xtrt r um rom D. Sop n (1, 2 n 4 /l) lvls wr to vr. Pysoml, mrol n snsorl tsts v n ppl. Rsults: Rsults nt tt ph, ty, olor n turty v n n y um ton wl smntton rt s n ru tt rsult mor stl vr. T rltonsp twn smntton rt n vsosty lso s n nvstt w sow otr tors mor tn vsosty wr tv n smntton rt. Inrsn sorpton o mtrx urn t tm sow tt ssolv mtrl s n nrs ovr t tm. Mrol nlyss sow nrmnt urn stor. Snsory vluton nt tt ommrl ums v no snnt t on ptlty o vrs wl D. Sop`s um s unsrl t on t. Rommn ppltons/nustrs: Aorn to t otn t, t ws trmn tt mxtur o xntn ur um n xntn um n 0.05/l lvl t st t on t D. sop prtls stlzton. 121 ss ontnsluosnolts w nlu llylsotoynt, utynnsotoynt, yno 3 n 4-ptoutn, 5-mtyltopntnntrl, 3- pnylpropontrl, 4-mtylto utyl sotoynt, n 2-pnyltyl sotoynt. Otr ompouns nlu plmt tty, lnoln s, ol s, n str s. T mnrl ompouns n t s nlu sulur, lorn, posporus, ron, potssum, lum, soum, n mnsum. T s lso

2 Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , 2017 ontns protn, um, mul, t, n ntron ompouns (Anr t l., 2012). In toy s worl us o t oo sours s on t onrns o mnn n mnn s loon or vlopn nturl rsours w s lwys on o t mportnt spts o t stuy. In ts stuy w r loon or wy to stlz td. sopvr or ommrl prouton. Amon t sn o t vrs n trtonl mnl rs l D. sop v mny ppltons us o tr usul proprts. D. sopn t trtonl mn, s wrm ntur r (s wrm umor) possssn so mny proprts su s ln wouns n nurs, ntpyrt, rr trtmnt, ntlmnt n trtmnt o ny stons, trtmnt o ny o vtmn C, n snton proprts. Howvr, s w now, spt vn so mny usul proprts n nutrton, ts vr otn u to l o nustrl prouton sn t n n t ntrst o t oo nustry so r, n t sms tt on o t mn prolms to o so s nstlty o t vr tr prouton (Al-Jr, 2011; Anrt l., 2012; Kn n Wn, 2012) Dspt molulr wt xntn um ssolvs rly n ol n wrm wtr n vn n vry smll mounts prous onntrt solutons n ph ns osn t v mu t on t. T mn us o xntn s s tnr, stlzr, n vsosty mor nt n n ntrtn powr mxturs n n stlzn t mulson (MommHsm, 2011; Wstlr nbmllr, 1992) In ton to tnn, son, n vlopn stlty, Croxy Mtyl Cllulos or CMC s lso uson nt, wtr mntnn nt, ollol stt mntnr, stlzr,suspnn nt, mulsr, n lyr onstruton nt. Ts sustn ssolvs rly n ol n wrm wtr n s us slly n rumstns wr vsosty ontrol s mportnt. T t tt ts sustn s vsosty ontrol nt s m t to l to us s tnr, stlzr, mulsr (l sn) n suspnn nt. Gur um n low onntrtons prous vsous solutons n ts 2-3% onntrton prous l. Ts um osn t sow ny nomptlty to protns or otr polysrs. Flty o t ur us n t nustry, u to ts uson n rp yrton n ol n wrm wtrs, s wll s us o t sustn to otn propr onntrtons n somtms nrs n wtr sorpton n t prouts t. Aorn to wspr us o ts sustn n w rn o mulsrs, sotnrs, n stlzrs (Wstlr n Bmllr, 1992).In lr rsrs, ty r to t onluson tt ntv ums, n t prsn o ron ons, n stlz s wll s nr t Flxwvr wr ts snsory rtrsts ws vry smlr to t unstl rulr vr.bs on t nns o notr stuy, wtr-solul rtons o um trnt n Prsn um n torz s non yroollos w r sor on t sur o sns w n prvnt rton v str n ltrostt rpulsons y smultn ry lyr. In ton, tr nsolul rtons oul promot pysl stlty o t mxturs u to mn ll ntwor n nrsn vsosty. 2. Mtrls n Mtos D. sop ss wr otn rom t lol mrt, Srz, Irn n ty n purs rom Brn, Irn. Xntn, CroxyMtylCllulos (CMC) n ur ums rom Mr Compny (Drmstt, Grmny), wr prpr rom rsr ntr o Isn Unvrsty o tnoloy s powr n D. sop um n xtrt n t wy w wll xpln ltr. Ctr n lmon ssn prpr rom Srz Zmzm tory. QL s rowt mum plt ount r ws us or mrol vluton. In ts rsr, D. sop um,xntn, CMC n ur ums n rnt onntrtons wr us to p D. sopvr stl n prvnt smntton o ts ss. Pysoml, mrol (rst n n o t two monts stor), snsory (or ot two ormulton typsvlutons wr on. Pysoml vlutons nlu ph, ty, olor, turty, prtl sz, smntton rt (or ot ormulton typs), n sptropotomtr (sorpton n FT-IR) msurn to otn vr soluton s olor n D. sop um s ml strutur. Otr 122

3 Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , 2017 tn t mnton ov, otr vlutons wr on or t ormulton 2. D. sop vr prprton mto For ts purpos 15 D. sop n 25 sur wr us n 250 ml o wtr. Tror t ov mnton quntts n xprss n prnts, too (Tl 1). D. sop um prprton n xtrton mto D. sop, rns n wrm to C n wtr t n tr ooln, t ws pt n t rrrtor or 3-5 ys. T D. sop tn ws ltr wt lot or srn. Atr rottn, t otn xtrt ws pour on pln sur l try n pt n n ovn t C to ry. T r um ws tn ut o rom t try y sptul. Anlyss o pysoml omposton Sptropotomtr vluton Dtrmnton o mxmum Asorpton rquny (λmx) Smpls wr pour nto qurtz ll n tr sorpton nm wvlnts wr r y sptropotomtr (Kontron s Bo TUvKon 923,Enln). E smpls sorn ws lult onsrn t wvlnts w mxmum sorpton n tn ts mount ws rsn (Soon Wst, 1997) T mxmum sorton rquny s n lult orn to Soo s prnpls o nstrumntl nlyss oo n ts wvlnt w ws qul to 320 nm ws st on (Srwoo 320, Enln) sptropotomtr or susqunt sptropotomtr vlutons n ll t smpls sorpton wr r on vry 2 ws or 2 monts wt 10 tms luton. Fourr Trnsorm InrR (FT-IR) FT-IR sptrum o pur um ws otnn orr to nty ons n ml struturs. T qupmnt lso s t lty to nty sur onn. In ts mto, lt s rt to t smpl's sur n t prnt o t lt trnsmsson or 123 lt sorpton wll msur y t qupmnt, n on typs n mtrl strutur wll trmn trou xstn sours. T smpl ws norport nto KBr (sptrosop r) n prss nto 2 mm pllt. IR sptr wr ror n t trnsmttn mo rom 4000 to 450 m -1. For ts purpos w us sorpton sptromtry (Prn Elmr Atom,US) (PrrnIruyr, 2002; FrnynAsr, 2010). Msurn olor ntors Color proprts o t prpr D. sopvrs wr ssss y Colorolx EZ (Huntrl, Vrn, USA), SI (sturton nx) (Eq1),u nl (Eq2),ΔE (totl olor rn) (Eq3), *(+, r; -, rn), L* (Ltnss nx) n *(+, yllow; -, lu)ntors wr n (BnnAs, 2014; SronnYlmz, 2010). SI = (1) H = rtn ( ) (2) E = (L 0 L ) 2 + ( 0 ) 2 + ( 0 ) 2 (3) Turty msurmnt It ws msur y turty mtr v s on FTU (HI HANNA, US) (MuturmnnSsl, 2012). ph vluton By usn ph mtr (Mtrom, 827 v,swtzrln), tr lrton wt ur soluton 7 n 4, v s ltro ws put n t smpl, n t t ws wrttn tr t ws x (StnHossn, 2011). Ts vluton ws on on vry 2 ws or 2 monts n or ot ormultons. Aty vluton y ttrton mto In ts vluton, rs 0.1 N soluton ws prpr n tn 25 o t smpl ws ttrt wt t n 3 roplts o pnolptln ntor wr us tll t turn nto purpl, tn t mount o soumus ws r, put nto ty quton n tr n t ty ws n n Dorn r y quton 4(Stn Hossn, 2011).

4 Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , 2017 Aty= S Wr: (4) volum o t soum us S 25 ml (volum o t smpl) on ml N soum s qul to tr 10 Stlzton o D. sop n vr y ums Formulton 1 15 o D. sop ws rns, r to 250 volum n 25o sur ws to t. It ws t to 40 C y ot plt mnt (trml mnt strrr) n tn t ums wr tr rn ts tmprtur n t strrn ws ontnu untl 50 C n omplt ssoluton o t ums (Tl 2). Formulton 2 15 o D. sop ws rns, r to 250 volum n 25 o sur ws to t. It ws t to 40 C y ot plt mnt (trml mnt strrr) n tn t ums wr tr rn ts tmprtur n t strrn ws ontnu untl 50 C n omplt ssoluton o t ums. 0.1% tr (orn to numr 2837 stnr) n tn 0.05% lmon ssn (FCC t ton 2014) ws to t. T m soluton ws pour nto l lss n ws put n wtr t (75 C, 15 s) or psturzton (FDA), n tr ooln own ws pt n t rrrtor t 4 C. In orr to p smpls n t rrrtor or 2 monts, t psturzton ws on n tr ws us to ru ty n orr to ru mrol rowt n lmon ssn ws us to lvor t vr n msn t lvor (Tl 2). Prtl sz vluton Ts vluton ws onut y optl tl mr wt 2.5 zoom. For ts purpos, som o smpl ws pour nto plt n put n ontnr n ws potorp y t mr. T sur, mxmum, mnmum, n vr prtls mtr, 124 n tr sprty wr tn v y m prossn prorm. Smntton rt vluton In ts vluton smpls wr put nto msur n t smntton tm to l msur volum ws prtlly msur y ronomtr. T smntton rt (V) ws lso tortlly lult y Stos lw n nlly t tortl n prtl vlu wr ompr (Forount l., 2009). T omplt srpton o Stos lw (q.5) sows us tt prtl s sp sttln nto lu s rtly rlt to rvty (), t rn twn prtls n wtr nsty (D 1-D 2), n t squr o t prtl s rus. T smntton rt s nvrsly rlt to lu s vsosty or tnss or lqu s vsosty (η). V 1 = 2r2 (D 1 D 2 ) (5) η Wr: V: smntton rt n ntmtr pr son : rvty lrton, w s onsr qul to 980 m pr squr son. r: rus o t prtl sttln n t lqu n m. D 1: tul wt, w s onsr n /m 3. D 2: wtr nsty, w s qul to on /m 3. η: lqu s vsosty w s onsr qul to 1P(0.01 /m.s)or wtr. Du to mportn o t rus n ts quton prtl sz t on smntton rt wll suss s ollows. Mrol vluton In orr to nty prmry smpl n prout s mros tr 2 monts stor n t rrrtor, totl ount vluton ws on wtplt ount r (PCA) ultur mum (QL s) n 10-1, 10-2, n 10-3 lutons n n uplt(irnn Ntonl Stnrzton Ornzton). Evluton o som snsory proprts Som o snsory proprts o t smpls (tst, pprn, mout l, olor, n t nrl msson) n 6-pont Hon vluton rmwor ws vlut s vry utlty, utlty, low

5 Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , 2017 utlty, low sutlty, sutlty, n vry sutlty y 30 trn pnlsts. T tst vluton rsults lso sow tt t vr ponts o t stlz systms y rnt prts o t xntn n CMC ums wrn t so rnt rom t ontrol smpl (D. sopvr wtout um), vn n som ss t pnlsts vlut tm mor srl (BnnAs, 2014). 3. Rsults n susson Sptropotomtr vlutons All t smpls wt wvlnts twn nm t mxmum sorpton vlu n orn t rps otn rom t v t 320 nm wv w ws on top o t rps ws onsr t ln mx. By usn n sorptons urn 2 monts, t ws u tt t sorpton vlu nrss n tm n lso t ws n ts mxmum t t lst (ourt) w n n som ums l D. sop u to n romt, n xtnt nrs t t n o t ourt w. Ts nrs n sorpton s us mor sustn s n ssolv n t soluton n t romt sustns u to ums wr prmt nto t soluton (Soon Wst, 1997). Colormtr msurmnt Control trtmnt mor trnsprny n lrty n ums ru D. sopvr s trnsprny. D. sop n xntn ums t most nlun on t trnsprny ruton n CMC um t lst nlun n ts mttr w n t ws prtl vn t CMC um n xntn proprts n D. sop um olor. Unl rsults otn rom Bn n As (1393) stuy w L* nx ws twn 27-32, L* nx otn rom t tsts wr vrl n rn w mpls smpls mor trnsprny n ompr to tt stuy. In t t L* vlu otn rom t lst stuy sow lot o turty n t vr. Aorn to t * nx, * n * nx ws lso u tt ontrol trtmnt n CMC t lst vlu n n D. sop n xntn um trtmnt t most vlu u to t mnton rsons 125 (BnnAs, 2014; SronnYlmz, 2010). As t s trmn wt otr nx trtmnts totl olor rn ws mnmum n t ontrol trtmnt u to t l o um n ts rn s rtr n t trtmnts wt mor turty tn t ontrol trtmnt su s trtmnts ontnn D. sop n xntn um. Aorn to t ov rsults t u nl nx lso t mxmum vlu n ontrol trtmnt n trtmnts ontnn CMC n lss vlu n D. sop n xntn um. Turmtr vluton As t ws rsult rom turmtr vluton, ontrol trtmnt t mnmum turty n trtmnts ontnn D. sop um t mxmum turty u to t um olor n vn ollol sustns n n ll t smpls turty nrs wt um onntrton nrs, w ws prtl (Tl 3). ph vluton Tm n t v snnt t on ph, t mns tt yron on onntrton n t n n ph ws pt n t rn. Ts ph rn s ustl orn to t vrs stnr (Irnn Ntonl Stnrzton Ornzton, 2010). Aty vluton Gvn t ty vluton t s u tt ty ns urn 2 monts ws out 0.2 D n t mount o t onsum NOH vry lttl n, too (Irnn Ntonl Stnrzton Ornzton, 2010). In t ph n ty rsults sow tt ts two tors t oorntly n no t on t onsum ums n lso t rrrtor tmprtur pt ts two tor n rtn rn. Cns n ty v n xprss n tl 4.

6 Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , 2017 Tl 1. Numr, nm, n onsttunt omponnts o t smpls Numrs Nms & onsttunt Symols Symols n s Prnt o omponnts 1 Xntn um Xn X % 2 Xntn um Xn X % 3 Xntn um 0.05 Xn 0.05 X % 4 Croxy Mtyl Cllulos CMC C % 5 Croxy Mtyl Cllulos CMC C % 6 Croxy Mtyl Cllulos 0.05 CMC 0.05 C % 7 Xntn Gur Xn + Gu X+G 0.01% 8 Xntn Croxy Mtyl Xn + CMC X+C 0.01% Cllulos Bln Bln Bln 0% 10 D. sop um 0.25 Sl 0.25 S % 11 D. sop um 0.5 Sl 0.5 S % 12 D. sop um 1 Sl 1 S1 0.4% Tl 2. Formultons n rns Formultons Drns 1 15 o D.sop 250 wtr 25 o sur ums o D. sop 25 wtr 25 o sur ums 0.1% tr 0.05% lmon ssntl ol psturzton Tl 3. Comprn turmtr n olormtr vlutons vr Smpls L * * * C * ΔE SI Turty (FTU)

7 Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , Mns (± stnr vton) wt smll ommon lttrs osn t v snnt rns t 5% lvl n Dunn. Tl 4. Aty ns n D. Sop vr urn t stor Trtmnt Frst w Son w Tr w Fort w Tl 5. Tortl n prtl smntton rt omprson n ot ormultons Formulton 1 Formulton 2 Smpls Tortl Prtl Tortl & prtl rto Tortl prtl Tortl & prtl rto Xn Xn Xn CMC CMC CMC Xn + Gu

8 Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , 2017 Xn + CMC Bln Sl Sl Sl F. 1. FT-IR sptrum o D. sop powr Fourr Trnsorm InrR (FT-IR) Aorn to t FT-IR sptrum (F. 1), t ws oun tt: In t m -1 r, t D. sop um s n lool nt, prsn o t sorpton ps n ts r s usully us o t tnsl vrton twn yron n notr tom. T sorpton ps n m -1 r vn tr wnss r rlt to t lool. In t m -1 r tr r CH Alpt rlt vrtons. In t m -1 sorpton ps r u to C = C tnsl vrtons orn to w usul normton out Oln s struturs n n. In t m -1 r s sorpton sptr smll rns n molul s strutur n omponnts rsult n mor ns n sorpton ps struton. Bus o ts omplxon rul ntrprtton o ts r s sptr s rly possl. In nrl, u to l o ronyl n roxyl nt ts um sn t snstv to ph n onzton osn t v ny t on t, so t s not pnnt on ph (PrrnIruyr, 2002; SoonWst, 1997). Prtl sz vluton Aorn to Stos' lw, prtl sz n t t smntton rt, tn t t o ums on mtr o D. Sop`s mtr s n nvstt n sow tt smntton rt nrs s prtl sz ru. 128

9 lo CFU/ Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , 2017 Smntton rt vluton Aorn to t Stos s lw ormul (Eq.5) t s u tt vsosty nrs rsults n smntton rt rs n t s xpt tt vsosty nrs woul ly prtls smntton, ut smntton s pnnt on otr tors too, tt y usn t t otn rom prtl smntton rt vluton towrs t ontrol smpl, t ws u tt t s onsstnt wt t Stos s lw n t mxmum vsosty osn t nssrly v t mnmum smntton rt ut wtr sorpton y t prtls, nrs n tr mtrs (prtl s sz vluton), yroynm rus, vlopn l ntwor, synrst stt, n ttrton n rpulson twn tr prtls n rsult nto stlty nrs n smntton rt rs. Ts onluson s onorn wt Foroun t l. rsr n 1388 w ws stu out oo (n Irnn yourt vr) (Forount l., 2009). In t Stos s lw tory towrs t ontrol smpl, t vlu ws nr 1, t oul s tt smntton rt ru s u to vsosty nrs. In t ormulton 2 w n ssn wr us smntton rt prtlly nrs n ompr to t ormulton 1. Aorn to Stos s lw tortl n prtl rto n ot ormultons s sm s Tl 5: Mrol vluton T mrol vluton rsults sow tt (F.2) t t y 0 t xtrt trtmnts ontnn D. sop ums t mxmum olony vlu u to t xtrton ontons n t mnmum olony rowt ws sn n t trtmnts ontnn xntn n CMC. T numr o t trtmnts ontnn xntn n CMC olony nrs n tm ut t ws lttl ru n t trtmnt ontnn D. sop um w n us o t tr n nutrnts n loss o tm. At t n o t stor pro t mount o t olons rowt n ll t trtmnts ws mor tn t stnr lmt (3.0 ± lo CFU/).Bus psturzton t trtmnt ws us or ts vr, ts stor tm ws ovrunn or t n t optmum stor tm soul lss or stronr t trtmnt, l strlzton, soul us (Irnn Ntonl Stnrzton Ornzton) F.2. Btrl rowt t y 0 n y 60 T ommon lttrs mply nxstn o t snnt rn t 5% lvl Snsory vluton In ts vluton, orn to t ormultons 1 n 2 t ws u tt xpt D. sop um,w ws vn y mor prnt to t D. sopvr n ompr to otr ums, otr ums n t v mu t on smpls tst, olor, smll, mout l, n nrl msson n t smpls tst y vlutors wr pt. Also y pplyn psturzton t trtmnt to t sonry smpls tr wsn t ny ns n vr s snsory proprts w ws onsstnt wt Bn n As (1393) stuy (BnnAs, 2014; SronnYlmz, 2010). T rsults o snsory vluton v n nt n F trtmnts Dy 0 Dy

10 Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , 2017 olor nrl utlty F. 3. Snsory vluton o rnt trtmnts 4. Conluson lvour oor mout l Aorn to t vlutons mn t rsults n t ws trmn tt xntn ur um n xntn um n 0.05 lvl t mxmum t on t D. sop prtls stlztonatr xmnn smpls olor n turty t ws sn tt ums t olor n turty, splly, D. sop um w us o vn olor us turty n t prouts ut CMC um sow mnmum t on nrsn smpls olor n turty. In vlutn ph, ty, n sorpton urn 2 monts wly t ws sown tt ph n ty n t v mu n n vr n stnr rn ut sorpton nrs urn tm u to ssoluton o mor sustns n t soluton. D. sop um ompouns wr trmn y FT-IR vluton n t ws u tt ts um sn t pnnt on ph. Mrol vluton urn stor lso sow tt t xtrt D. sop um nrs mrol ontmnton u to xtrton stps n 2 monts stor wsn t sutl or ts prout so lss tm s srl or stor. Ts vr ws srl n trms o snsory proprts n xpt trtmnts ontnn D. sop um, otr trtmnts wr pt y vlutors. Foo nustrs r onstntly loon or onom n nnovtv wys o nrtn oo prouts wt mor srl txturl n ornolpt proprts to mt onsumrsrqurmnts so y stlzton o D. sopvr n us nw snsory rtrst prtly w n r to tt ol. 5. Rrns Anr M, Nmrouz M, B AH.Stuyn t Opnons n xprns o ram out trput ts o t trtonl prout surnsop.med Q J. 2012; 4: Al-Jr NA.Pytoml n olol stus o SsymrumroL. Su J. Cm So. 2011; 4: DOI.or/ /.ss Bn M, As S. Stlzton o Flxw (DsurnsopL.) syrup usn ntv yroollos. Irn J NutrS Foo Tnol. 2014; 9: Frny A, Asr H, Mzoo M, MsG. T mpt o onntrton, tmprtur n ph on ynm roloy o psyllum ls. Journl o Foo Ennrn.2010; 100: DOI.or/ /.oon Foroun S, As S, Hm Z. Inlun o Sr Strrn n Homonzton on Srum Sprton o Doo. EJFPP.2009; 3: Irnn Ntonl Stnrzton Ornzton numr Ml n ry prouts, Dtrmnton o ph n ty. Tst mtos Irnn Ntonl Stnrzton Ornzton numr Flxw, Sptons n tst mtos.1st Rvson. Irnn Ntonl Stnrzton Ornzton numr 6332.Spton o pvnt n o onntrt n spt n spt p.1st Rvson. Kn M, Wn N.Dsurnsop(L.): w wt multpl mnl uss.pun Unv. J. Zool. 2012; 27: MommHsm M. Stuy on t o Xntn n Ar um on nzymt tvty n untonl proprts o lysozym n ts pplton n oo systm. MS tss, Arultur Amy, Srz Unvrsty Muturmn G, Ssl S. Rmovl o turty rom rnn wtr usn nturl oulnts. J InEn 130

11 Hssnpour t l., Journl o Hrl Drus, Vol. 8, No. 2: , 2017 Cm. 2014; 20: DOI.or/ / Prr MM, Iruyr J.A rp FTIR sptrosop mto or stmton o n n sot rns n totl mtylxntns n t n o. J. Foo S. 2002; 67: DOI: / t08767.x Sron C, Ylmz MT. Molln t Ets o Prossn Ftors on t Cns n Colour Prmtrs o Coo Mtlls Usn Rspons Sur Mtooloy. Worl ApplS J. 2010; 9: St N, Hossn F. Evluton o pysoml n snsory proprts o PET ontnrs p lmon u. JFST.2011; 8: Soo DA, Wst D. Prnpls o Instrumntl Anlyss, 5t n. Nw Yor. 1997; pp Wstlr RL, Bmllr JN.Inustrl Gums Polysrs n Tr Drvtvs, 3r n. (Nw Yor), 1992; p