Endocytosis and Endocytic Network in Plants. Communication in Plants: Neuronal Aspects of Plant Life

Transcription

1 Endocytosis and Endocytic Network in Plants Communication in Plants: Neuronal Aspects of Plant Life Bonn, 29 November 2006

2 WHAT IS ENDOCYTOSIS? Endocytosis Endo (within) and Cytosis (cell) A process in which a substance gains entry into a cell without passing through the cell membrane Subdiving into three different types: 1. Receptor-mediated endocytosis 2. Pinocytosis 3. Phagocytosis

3 1. Receptor-mediated endocytosis 2. Pinocytosis 3. Phagocytosis

4 TYPES OF ENDOCYTOSIS Phagocytosis &

5 Signalling Endosomes Animal/Human Neurobiology Animal/Human Immunobiology McPherson P.S. et al. (2001) Signalling on the Endocytic Pathway. Traffic 2: Sorkin A, Von Zastrow M (2002) Signal Transduction and Endocytosis: Close Encounters of Many Kinds. Nat Rev Mol Cell Biol 3: Gonzalez-Gaitan Gaitan M. (2003) ) Signal Dispersal and Transduction Through the Endocytic Pathway. Nat Rev Mol Cell Biol 4: Miaczynska M. et al. (2004) Not Just a Sink: Endosomes in Control of Signal Transduction. Curr Opin Cell Biol 16: Le Borgne (2006) Regulation of Notch Signalling by Endocytosis and a Endosomal Sorting. Curr Opin Cell Biol 18:

6 Endosomes Control Trafficking and Localization of Receptors McPherson P.S. et al.. (Traffic( 2: , 2001)

7 Signalling Endosomes Between Neuronal Synapses and Nucleus McPherson P.S. et al.. (Traffic( 2: , 2001)

8 ... IS THERE ANY ENDOCYTOSIS IN PLANTS? The 80 and 90 of the 20th Century: No The High Turgor Pressure Does Not Allow Any Endocytosis In n Plant Cells!!!

9 ... IS THERE ANY ENDOCYTOSIS IN PLANTS? Baluška F, Šamaj J, Hlavačka ka A, Kendrick-Jones J, Volkmann D (2004) Myosin VIII and F-actin F enriched plasmodesmata in maize root inner cortex cells accomplish fluid-phase endocytosis via an actomyosin-dependent process. J Exp Bot 55: Šamaj J, Baluška F, Voigt B, Schlicht M, Volkmann D, Menzel D (2004) 04) Endocytosis, actin cytoskeleton and signalling. Plant Physiol 135: (Review) Voigt B, Timmers A, Šamaj J, Hlavacka A, Ueda T, Preuss M, Nielsen E, Mathur J, Emans N, Stenmark H, Nakano A, Baluška F, Menzel D (2005) Actin-based based motility of endosomes is linked to polar tip- growth of root hairs. Eur J Cell Biol 84, Baluška F, Liners F, Hlavačka ka A, Schlicht M, Van Cutsem P, McCurdy D, Menzel D (2005) Cell wall pectins and xyloglucans are internalized into dividing root cells s and accumulate within cell plates during cytokinesis. Protoplasma 225: Ovecka M, Lang I, Baluška F, Ismail A, Illeš P, Lichtscheidl IK (2005) Endocytosis and vesicle trafficking during tip growth of root hairs. Protoplasma 226: Wang Q, Kong L, Li Y, Šamaj J, Baluška F, Lin J (2005) Effects of Brefeldin A on pollen germination and tube growth: antagonistic effects on endocytosis and secretion. Plant Physiol 139: Šamaj J, Read ND, Volkmann D, Menzel D, Baluška F (2005) The endocytic network in plants. Trends Cell Biol 15: (Review) Šamaj J, Baluška F, F Menzel D (2005) Endocytosis in Plants. Springer Verlag (Book) Dhonukshe P, Baluška F, Schlicht M, Hlavačka ka A, Šamaj J, Friml J, Gadella Jr TWJ (2006) Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis.. Dev Cell 10: Baluška F, Menzel D, Barlow PW (2006) Cytokinesis in plant and animal cells: endosomes shut the door.. Dev Biol,, In press (Review)

10 Multivesicular Bodies (MVBs) Endosomes in Plants Winter and Hauser (Trends Plant Sci, In press)

11 Endocytosis: Plant Cell Nutrition Šamaj J, Baluška F, Menzel D (eds) Endocytosis in Plants (2005) Springer Verlag

12 Endocytosis in Plants 1. Plant Cell Nutrition 2. Cell Wall Remodelling 3. Tip Growth 4. Cytokinesis 5. Stomata Movements 6. Papilla Formation 7.. Innate Immunity 8.. Polar Auxin Transport 9.. Brassinosteroid Signalling 10.. Blue Light Perception Signalling 11.. Plasma Membrane Nucleus Signaling

13 Brefeldin A (BFA) Treatment Induces s BFA Compartments F-Actin-Dependent Endocytosis Drives Vesicle Recycling

14 BFA-Induced Compartment

15 Auxin Efflux Carrier (PIN1) Recycles At Cross-Walls Of Apical Cell Poles Of Root Cells B Control BFA PIN1 PIN1 A

16 BFA Compartments Cross Walls PIN2 PIN2 - BFA B Boonsirichai K et al - Plant Cell 15: (2003) A

17 Our bones are replaced via endocytosis... Bone-resorbing osteoclast Väänänen nen et al. (2000) what about plant cell walls?

18 Endocytosis: Cell Wall Remodelling Plant Physiol 130: (2002) Plant Physiol 130: (2002)

19 RGII Pectins In Maize Root Cells BFA 2h

20 RGII Pectins In BFA-Treated Maize Root Cells

21 Endosomes - PPBs Microtubules Endosomes PPB Dhonukshe et al (Dev Cell 10: , 150, 2006)

22 Endosomes Cell Plate Golgi Stacks Endosomes Dhonukshe et al (Dev Cell 10: , 150, 2006)

23 PEN1 SNARE Papilla Formation

24 PEN1 SNARE Papilla Formation Assaad et al (Mol Biol Cell 15: , 2004)

25 PEN1 SNARE Localizes to Endocytic BFA-induced Compartments and Cytokinetic Cell Plates PEN1-GFP line: Hans Thordal-Christensen (Boris Voigt)

26 PEN1 SNARE Localizes to Endocytic Clear Zone of Root Hairs PEN1 FM4-64 Control Cyt D PEN1-GFP line: Hans Thordal-Christensen (Boris Voigt)

27 FYVE-GFP Labelled Endosomes in Root Hairs Voigt et al (Eur J Cell Biol 84: , 621, 2005)

28 FYVE-GFP Labelled Endosomes in Root Hairs Accumulate at Outgrowing Bulges (b) and at Actively Growing Tips (c) Voigt et al (Eur J Cell Biol 84: , 621, 2005)

29 Ovecka et al (Protoplasma 226: 39-54, 2005) Endocytic Tracer FM1-43 in n Root Hairs

30 Plant Physiol 133: (2003) FM4-64

31 Fluid-Phase Endocytosis Of f Lucifer Yellow At t Plasmodesmata/Pit /Pit- Fields Of The Inner Cortex Cells PD CW PD CW Vacuole PD

32 Myosin VIII Actin Fluid-Phase Endocytosis Myosin VIII and Actin at Pit-Fields in Cells of the Inner Cortex Pit-Fields Pit-Fields Pit-Fields Pit-Fields Actin

33 Myosin VIII - Plasmodesmata Root Inner Cortex Cross-Section

34 Myosin VIII At Plasmodesmata/Pit-Fields In The Inner Cortex Young Cross- Wall CW Pit-Field Reichelt et al. (1999) Plant J 19:

35 Plasmodesmata Pit-Fields ields Plasmodesmata Plasmodesmata Grouped Into Pit-Field Pit-Field Evert et al. (1977) Planta 136: 77-89

36 Synapses Term was Coined in 1897 for Neurons by Charles Scott Sherrington Current Broader Definition: Asymmetric Actin-Based Adhesion Domains Specialised for Cell-to-Cell Communication Dustin ML, Colman DR (2002) Neural and immunological synaptic relations. Science 298:

37 Synapses A) Neuronal Synapses B) Immunological Synapses C) Virological Synapses D) Plant Synapses Baluška F, Volkmann D, Menzel D (2005) Plant synapses: actin-based adhesion domains for cell-to-cell communication. Trends Plant Sci 10:

38 Plant Synapses Endocytosis and Vesicle Recycling at Plant Synapses: From Auxin Transport to Signalling Baluška F et al (2003) Polar transport of auxin: Carrier-mediated flux across the plasma membrane or neurotransmitter-like secretion? Trends Cell Biol 13: Baluška F, Volkmann D, Menzel D (2005) Plant synapses: actin-based adhesion domains for cell-to-cell communication. Trends Plant Sci 10:

39 Plant Synapses: Actin and Myosin Actin Myosin VIII Zea mays Baluška F, Barlow PW, Volkmann D (2000) Actin and myosin VIII in developing root cells. In: Actin: A Dynamic Framework For Multiple Plant Cell Functions, CJ Staiger, F Baluška, D Volkmann, PW Barlow (eds), Kluwer Academic Publishers, Dordrecht, The Netherlands,

40 Effects of Brefeldin A (BFA) on F-Actin and Plant Synapses Zea mays Control BFA 2h

41 Endosomal Plasma Membrane/Synapse Nucleus Signalling in Plants: Trithorax ATX1 ATX1-GFP Alvarez-Venegas R, Sadder M, Hlavacka A, Xia Y, Lu, G, Firsov A, Sarath G, Moriyama H, Baluška F, Avramova Z (2006) Phosphoinositide-5-phosphate phosphate and the Trithorax homolog, ATX1, define a novel regulatory pathway in Arabidopsis. Proc Natl Acad Sci USA, In press

42 Plant Synapses Blue Light Photoreceptor PHOTOTROPIN OTROPIN 1 Arabidopsis thaliana GFP-PHOT1 PHOT1 Green, FM4-64 Red Yinglang Wan Collaboration with Winslow Briggs

43 The blue light receptor photropin 1 (Phot1) is a flavin-binding light-activated protein kinase. The blue light-induces induces endocytosis of blue light receptor photropin 1, which emerges as part of the signal transduction pathway. The most rapid blue-light light induced endocytosis of photropin 1 was scored at root synapses.

44 Innate Immunnity Receptor Kinase FLS2 is Internalized in Response of Binding to Ligand Flagellin and Its Endocytosis is Needed for Its Signalling

45 Endosomes in Self/Non-Self Recognition Goldraij et al (Nature 439: , 810, 2006)