HCV E1E2 : Assembly on infectious virions

Transcription

1 A cluster-based protein coevolution method uncovers critical features of the original fusion mechanism Comment l étude de la co-évolution des protéines virales a permis de révéler le mécanisme de fusion membrane du VHC E1E2 : Assembly on infectious virions E1 et E2 Pre-fusion structure 1

2 E1E2 : Interaction with receptors E1E2 : Internalization within cells Endosome E1 et E2 Pre-fusion structure Endosomal membrane Viral membrane 2

3 E1E2 : Membrane fusion Viral membrane Endosomal membrane Cytoplasma E1 et E2 Post-fusion structure E1 and E2 functions are poorly characterized E1 E2 flavivirus YFV BVDV CSFV pestivirus Den WNV JEV pegivirus GBV-A Clade H,G GBV-C GBV-B hepacivirus Transmembrane domains Adapted from Krey et al,

4 Dengue envelope glycoproteins E1 E2 Dengue E Dengue PrM DIII Dengue E domains DII DI Class II fusion protein-induced fusion E1 E2 Dengue E Cell surface binding Membrane fusion Dengue PrM Structural chaperone Cell Virus Homodimer dissociation Virus genome Domain III Fold Back Membrane merging Fusion 4

5 E2 is not a Class 2 fusion protein hepacivirus E1 E2 flavivirus YFV BVDV CSFV pestivirus Den WNV JEV pegivirus GBV-A Clade H,G GBV-C GBV-B hepacivirus Kong et al. Law, Science 2013 Khan Marcotrigiano Nature 2014 El Omari Stuart Nat Com 2014 hepacivirus E1 E2 E1 and E2 functions are still poorly characterized BL β-s Kong et al. Law, Science 2013 Khan Marcotrigiano Nature 2014 El Omari Stuart Nat Com 2014 FL 5

6 fusion mechanism: how do E1 and E2 act? hepacivirus E1 E2 flavivirus Dengue E pestivirus BVDV E1 E2 Dengue PrM DIII DI DII Binding: E2 Fusion: E1? Binding: E Fusion: E Binding: E2 Fusion: E1 Hepatitis C Virus E1E2 coevolution networks analysis unveils their functional dialog and structural organization Identification Molecular virology Theoretical modelling Function, molecular entry mechanism Application Entry inhibitors developement 6

7 Mediator of protein function Protein coevolution signals are strong mediator of protein functions. Such signals represent interesting tools to unveil viral protein dynamic rearrangements and uncover potential therapeutic targets. Loss of function Protein coevolution signals are strong mediator of protein functions. Such signals represent interesting tools to unveil viral protein dynamic rearrangements and uncover potential therapeutic targets. 7

8 Restoration of function Protein coevolution signals are strong mediator of protein functions. Such signals represent interesting tools to unveil viral protein dynamic rearrangements and uncover potential therapeutic targets. The BIS Model A computational detection of amino acid co-evolution * Allows identification of networks and dialogs within proteins * Principle: Small protein fragments can be used as basic building blocks to reconstruct networks of coevolving amino acids Ø Can we identify E1E2 functional dialogs faster and in a larger extend? Ø Can we predict potential E1E2 structural organization and rearangements? Dib L and Carbone A. PLoS One

9 Application of BIS model to two envelope glycoproteins: E1E2 Genotypes and sub-types 1a 1b 1 2a 2b 2 3 4a 5a 6a Number of sequences processed Number of clusters detected Clusters location Total detected Total (p<0.05) intra-e1 (p<0.05) intra-e2 (p<0.05) inter-e1-e2 (p<0.05) Subtypes (15-30%) Types 1 to 6 (>30%) 6a 6b 4a 4 3c 3b 3a 2a 2c 2b a 1b 1a 1c 0.1 Clusters map important residues described in the literature Douam et al. In press. 9

10 E1E2 clusters map important residues described in the literature Genotype 1a clusters Structural Clusters (folding, heterodimerization, binding) Membrane fusion Clusters Multifunctional Clusters Undefined role 2 X 6 X X 11 X 5 X 7 X X 8 X 10 X X 12 X X 4 X X X 16 X X X 3? 9? 13? 14? 15? Douam et al. In press. fusion cluster support E1E2-interdependent rearrangements Douam et al. In press. 10

11 fusion cluster support E1E2 interdependent rearrangements Douam et al. In press. Soluble BL inhibits entry Douam et al., In Revision 11

12 Acknowledgements EVIR lab CIRI Florian Douam Floriane Fusil Dimitri Lavillette Fouzia Amirache Ornéllie Bernadin Bertrand Boson Solène Denolly Margot Enguehard Nelly Fontaine Natalia Freitas Christelle Granier Camille Levy Didier Nègre Anaïs Ollivier Chloé Mialon Jimena Perez-Vargas Els Verhoeyen Laboratoire de Biologie Computationnelle et Quantitative Sorbonne Université Alessandra Carbone Linda Dib Francesca Nadalin A protein coevolution method uncovers critical features of the Hepatitis C Virus fusion mechanism. Florian Douam, Floriane Fusil #, Margot Enguehard #, Linda Dib #, Francesca Nadalin #, Loïc Schwaller #, Gabriela Hrebikova, Jimmy Mancip, Laurent Mailly, Roland Montserret, Qiang Ding, Carine Maisse, Emilie Carlot, Ke Xu, Els Verhoeyen, Thomas F. Baumert, Alexander Ploss, Alessandra Carbone $, François-Loïc Cosset $ and Dimitri Lavillette $ PLoS Pathogens. In press. 12