PCR- TTGE PCR (PCR-TTGE) PCR.

Transcription

1 (-) (tryptic soy broth) TSB

2 - Typhi (-) 2500 enterica enterica Typhimurium (tryptic soy broth) TSB enterica 2500 bongori enterica Typhimurium Typhi Namimatsu et al., 2000 Olsen et al., 2000 Lyons et al., 980 Mahon et al., 997 Schonenbrucher et al., 2008 Kauffmann, Kauffmann-White RNA (Temporal Temperature Gradient gel. multiplex Lin and Tsen, 996 6S r multiplex -Temperature Gradient Gel Electrophoresis) - enterica Myers et al., 985 GC-clamp Salmonellosis 2- serotype - typhoid fever 4- gastroenteritis 5- serotyping

3 TGGE 99 Yoshino Electrophoresis) Yoshino et al., 99 enterica PTCC: 609 Typhi ATCC: 4028 Typhimurium 7 TSB (tryptic soy broth Dublin 2000rpm ml µl 2000rpm 20c Pui et al., 20 SalR SalF 40 Allel ID Aabo et al., 99 enteric 20 µl Sheffield et al., x0 8 CFU/ml BIONEER DCode universal mutation detection system Dcode 5 mm 6x6cm DAN 0 4 bp 254 Typhimurium Ladder - Dublin Typhi Typhimurium 2 x x bp x0 5 2 x x0 2 x

4 4 - Typhi Dublin enteric Typhimurium 4 PAGE S 6S r 9 B PAGE enteric Anderson Typhimurium 8 A Derby DGGE DGGE PAGE DGGE Anderson. P.N. et al., 200 Myers et al., 987 TGGE DGGE enteric DGGE 2r Aabo, S., Rasmussen, O. F., Rossen, L. &Sorensen, P. D. a. O., J.E. (99). identification by the polymerase chain reaction.. Molecular and Cellular Probes 7: Anderson. P.N., Hume, M. E., Byrd, J. A., Hernandez, C., Stevens, S. M., Stringfellow, K. &Caldwell, D. J. (200). Molecular analysis of serotypes at different stages of commercial turkey processing. Poultry Science Anderson. P.N., Hume, M. E., Byrd, J. A., Hernandez, C., Stevens, S. M., Stringfellow, K. &Caldwell, D. J. (200 ). Evaluation of repetitive extragenic palindromic-polymerase chain reaction and denatured gradient gel electrophoresis in identifying serotypes isolated from processed turkeys. Poultry Science 89: Kauffmann, F. (96). The species definition in the Enterobacteriaceae. International Bulletin of Bacteriological Nomenclature and Taxonomy : 5-6. Lin, C. K. &Tsen, H. Y. (996). Use of two 6S targeted oligonucleotides as primers for the specific detection of in foods. Journal of Applied Microbiology 80: Lyons, R. W., Samples, C. L., DeSilva, H. N., Ross, K. A., Julian, E. M. &Checko, P. J. (980). An epidemic of resistant in a nursery: animal-to-human spread.. JAMA 24: : Mahon, B. E., Ponka, A. &Hall, W. N., et al. (997). An international outbreak of infections caused by alfalfa sprouts grown from contaminated seeds. J Infect Dis 75: Myers, R., Fischer, S., Lerman, L. &Maniatis, T. (985). Nearly all single base substitutions in fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis. Nucleic Acids Res : 45. Myers, R. M., Maniatis, T. &Lerman, L. S. (987). Detection and localization of single base changes by denaturing gradient gel electrophoresis. Methods Enzymol. 55: Namimatsu, T., Tsuna, M., Imai, Y., Futo, S., Mitsuse, S., Sakano, T. &al, e. (2000). Detection of by using the hybridization in microtiter wells. The Journal of Veterinary Medical Science colorimetric /rrna sandwich 62:

5 O'Regan, E., McCabe, E., Burgess, C., McGuinness, S., Barry, T., Duffy, G., Whyte, P. &Fanning, S. (2008). Development of a real-time multiplex assay for the detection of multiple serotypes in chicken samples. BMC Microbiol 8: 56. Olsen, S. J., MacKinnon, L., Goulding, J., Bean, N. H. &Slutsker, L. (2000). Surveillance for foodbornedisease outbreaks: United States, Morbidity and Mortality Weekly Report CDC Surveillance Summary. 49: -62. Pui, C. F., Wong, W. C., Chai, L. C., Nillian, E., Ghazali, F. M., Cheah, Y. K., Nakaguchi, Y., Nishibuchi, M. and Radu, S. (20). Simultaneous detection of spp., Typhi and Typhimurium in sliced fruits using multiplex. Food Control 22: Schonenbrucher, V., Mallinson, E. T. &Bulte, M. (2008). A comparison of standard cultural methods for the detection of foodborne species including three new chromogenic plating media. International Journal of Food Microbiology 24: Sheffield, V. C., Cox, D. R. &Lerman, L. S., and Myers, R.M. (989). Attachment of a 40-base-pair G+C-rich sequence (GC-clamp) to genomic fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc. Nati. Acad. Sci.USA 86: Wray, C., McLaren, I., Parkinson, N. M. &Beedell, Y. (987). Differentiation of typhimurium DT204c by plasmid profile and biotyping. 2 Veterinary Record: Yoshino, K., Nishigaki, K. &Husimi, Y. (99). Temperature sweep gel electrophoresis: a simple method to detect point mutations. Nucleic Acids Research 9: