Development and Evaluation of Visual Biosensors for Rapid Detection of Salmonella spp. and Listeria monocytogenes
|
|
|
- Allyson Carroll
- 6 years ago
- Views:
Transcription
1 Development and Evaluation of Visual Biosensors for Rapid Detection of Salmonella spp. and Listeria monocytogenes Lawrence D. Goodridge Department of Animal Sciences Colorado State University
2 Introduction Ongoing foodborne outbreaks have necessitated the development of modern detection methods Most methods (cultural, immunological, molecular) are laboratory based, and not suited for field based testing Need to develop field based tests, that can rapidly determine the presence of pathogens in foods The perfect test: sensitive, specific, rapid, robust, easy to use (integrated), cost effective
3 Objective To develop a suite of optical biosensors for rapid detection of the foodborne pathogens Salmonella spp., and Listeria monocytogenes Optical biosensors: no need for instruments to read the test result (field deployable)
4 Optical Biosensor The Phast Swab Biochemical test Vertically integrated Contains a sampling device, bacterial growth media, immunomagnetic separation beads, and an enzyme substrate in a single device
5 Salmonella spp. Biosensor based on detection of a Salmonella specific esterase (apee) Outer membrane protein encoded by the apee gene (Collin-Osdoby and Miller 1994) Enzyme substrate:5-bromo-6-chloro-3-indolyl-caprylate (Magenta-Caprylate) Cleavage of the substrate results in the formation of a magenta precipitate
6 Salmonella spp. 20 hour test 13 hours of enrichment, IMS (10 minutes) 7 hour color development
7 Salmonella spp. Detection of Salmonella in artificially spiked poultry (thigh) Overnight culture of S. Enteritidis 10 fold serial dilutions Individual 11 X 11 cm 2 thigh portions (with skin on) inoculated with 1ml of each dilution Poultry allowed to dry for 20 minutes Each thigh portion swabbed with an individual Phast Swab Assay conducted as described
8 Salmonella spp.
9 Salmonella spp. Salmonella Phast Swab detected 10 0 CFU/121 cm 2 of poultry (thigh) within 20 hours Detection limit (sensitivity): 10 7 CFU/ml Specificity: Salmonella Typhimurium, Salmonella Enteritidis: positive E. coli O157:H7, E. coli K12, L. monocytogenes: negative Color formation was not even (possibly due to fat content of the poultry) Alternative sampling methods
10 Listeria monocytogenes Biosensor based on detection of phosphatidylinositolspecific phospholipase C (PI-PLC) Plays a critical role in escape of this human pathogen from host cell vacuoles Isolates lacking PI-PLC much less virulent (Camilli et al. 1991) Enzyme substrate:5-bromo-4-chloro-3-indoxyl-myoinositol-1-phosphate (X-inp) Cleavage of the substrate results in the formation of a soluble blue compound
11 Listeria monocytogenes 15 hour test Enrichment for 10 hours, IMS (10 minutes) Addition of lysis buffer (PI-PLC is intracellular), substrate Color development for 5 hours
12 Listeria monocytogenes Detection of L. monocytogenes in artificially spiked RTE turkey meat Overnight culture of L. monocytogenes 10 fold serial dilutions Individual deli slices (100 mm diameter) inoculated with 1 ml of each dilution Meat allowed to dry for 20 minutes Each deli slice swabbed with an individual Phast Swab Assay conducted as described
13 Listeria monocytogenes
14 Listeria monocytogenes L. monocytogenes Phast Swab detected 10 2 CFU per RTE deli slice of turkey (100 mm diameter) within 15 hours Detection limit (sensitivity) 10 6 CFU/ml Specificity: 55 of 57 L. monocytogenes isolates postive, E. coli O157:H7, E. coli C, Salmonella spp. negative Future work: investigate the use of the Phast Swab to test for environmental sources of L. monocytogenes (within RTE meat processing plants) L. monocyotgenes is ubiquitous and is considered an indicator of the cleanliness of these plants
15 Summary The Phast Swab is an easy to use test that can be completed in the field and requires minimal instrumentation Future work: make the test more sensitive by converting it to a fluorescent version
16 Acknowledgements Jeff Callaway Steve Higham National Pork Board #
Use of the 3M Molecular Detection System for Salmonella and Listeria spp.
Use of the 3M Molecular Detection System for Salmonella and Listeria spp. March 11, 213 Prof Steve Forsythe Pathogen Research Centre, School of Science and Technology Nottingham Trent University Clifton
AOAC Method Comparison Study. Deli Turkey 1 CFU/25g & 5 CFU/25g
AOAC Method Comparison Study Deli Turkey 1 CFU/25g & 5 CFU/25g AOAC Guidelines for Matrix Validation - AOAC mandates two-tiered fractional recovery procedure 1 CFU/25g Low level, recovery target between
3M Food Safety 3M Petrifilm Salmonella Express System
3M Petrifilm Salmonella Express System 2 3M Food Safety 3M Petrifilm Salmonella Express System 3M Petrifilm Salmonella Express System is a qualitative test used for the rapid detection and biochemical
Progress on the biocontrol of foodborne pathogens on leafy greens with non-pathogenic microbes
Progress on the biocontrol of foodborne pathogens on leafy greens with non-pathogenic microbes M.O. Olanya and D.O. Ukuku USDA Agricultural Research Service, Eastern Regional Research Center, Wyndmoor,
Immunomicroarray for multipathogens
Funding period: Jan 3-Feb ultipathogen screening and /or confirmation via microarray detection Arun Bhunia, ark organ, Shu-I Tu Center for Food Safety and Engineering, Purdue University ERRC, Wyndmoor,
Antimicrobial Activity of Cinnamic Acid, Citric Acid, Cinnamaldehyde, and Levulinic Acid Against Foodborne Pathogens
University of Tennessee, Knoxville Trace: Tennessee Research and Creative Exchange University of Tennessee Honors Thesis Projects University of Tennessee Honors Program 5-2014 Antimicrobial Activity of
Phospholipase C Fluorometric/Colorimetric Assay Kit
Phospholipase C Fluorometric/Colorimetric Assay Kit Catalog No. KM0129 Detection and Quantification of Phospholipase C Concentrations in Biological Samples. Research Purposes Only. Not Intended for Diagnostic
C.M. Harris*, S.K. Williams* 1. PhD Candidate Department of Animal Sciences Meat and Poultry Processing and Food Safety
The Antimicrobial Properties of a Vinegar-based Ingredient on Salmonella Typhimurium and Psychrotrophs inoculated in Ground Chicken Breast Meat and stored at 3±1 C for 7 days C.M. Harris*, S.K. Williams*
NRL-Salmonella, Hungary. National Food Chain Safety Office Food and Feed Safety Directorate Erzsébet Adrián 29 May 2018
NRL-Salmonella, Hungary National Food Chain Safety Office Food and Feed Safety Directorate Erzsébet Adrián 29 May 2018 Structure National Food Chain Safety Office Food and Feed Safety Directorate Official
EN ISO validation study of the Assurance GDS TM Salmonella method
BIOCONTROL SYSTEMS Inc. 12822 SE 32nd Street BELLEVUE, WA 98005 USA NF VALIDATION Validation study according to the EN ISO 16140 standard: Summary Report EN ISO 16140 validation study of the Assurance
Dong-Hyun Kang, Daniel Y.C. Fung
International Journal of Food Microbiology 54 (2000) 127 132 www.elsevier.nl/ locate/ ijfoodmicro Short communication Application of thin agar layer method for recovery of injured Salmonella typhimurium
Bacterial Cell Surface Charge, Attachment and Decontamination on Melon Rind Surfaces
Bacterial Cell Surface Charge, Attachment and Decontamination on Melon Rind Surfaces Eastern Regional Research Center Dike O. Ukuku Ph.D. FSIT-ERRC-ARS-USDA Wyndmoor, PA 19038 Background Information Ability
Overview. Michelle D. Danyluk University of Florida. 4/14/14
Michelle D. Danyluk University of Florida mddanyluk@ufl.edu Overview Biological Hazards What is the pathogen of concern? Are all strains created equal? What pathogen is the most resistant to the lethal
Pr oject Summar y. Funded by The Beef Checkoff
Pr oject Summar y Seasonal effects on E. coli O157:H7, multi drug-resistant Salmonella, and Listeria monocytogenes prevalence and E. coli O157:H7 and Salmonella load on hides and carcasses at cow/bull
Robustness of a cross contamination model describing transfer of pathogens during grinding of meat
Downloaded from orbit.dtu.dk on: Jul 24, 2018 Robustness of a cross contamination model describing transfer of pathogens during grinding of meat Møller, Cleide Oliveira de Almeida; Sant Ana, A. S.; Hansen,
Validation of methods of analysis. Application in food microbiology
Application identification number: NF102 Publication date: 07 th October 2016 Validation of methods of analysis Application in food microbiology Requirements regarding comparison and interlaboratory studies
Colorimetric GAPDH Assay Cat. No. 8148, 100 tests
Colorimetric GAPDH Assay Cat. No. 8148, 1 tests Introduction Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a tetrameric enzyme that catalyzes glycolysis and thus serves to break down glucose for
Antimicrobial Effect of Buffered Sodium Citrate (BSC) on Foodborne Pathogens in Liquid Media and Ground Beef
J Food Sci Nutr Vol 5, p 9~ () DOI:.7/jfn..5..9 Antimicrobial Effect of Buffered Sodium Citrate (BSC) on Foodborne Pathogens in Liquid Media and Ground Beef Si Hyun Ryu and Daniel Y. C. Fung Department
CRISPR-SeroSeq: A Developing Technique for Salmonella Subtyping
Department of Biological Sciences Seminar Blog Seminar Date: 3/23/18 Speaker: Dr. Nikki Shariat, Gettysburg College Title: Probing Salmonella population diversity using CRISPRs CRISPR-SeroSeq: A Developing
Industry Learning in Salmonella Control
Industry Learning in Salmonella Control Dr. Angie Siemens Cargill www.cargill.com CONTROL: to exercise restraint or direction over; dominate; command. CONTROL: to eliminate or prevent the flourishing or
Performance Evaluation of Various ATP Detecting Units
Silliker, Inc., Food Science Center Report RPN: 13922 December 11, 2009 Revised January 21, 2010 Performance Evaluation of Various ATP Detecting Units Prepared for: Steven Nason 941 Avenida Acaso Camarillo,
SEC. Interpretation Guide. Select E. coli Count Plate
Interpretation Guide The 3M Petrifilm Select E. coli Count Plate is a sample-ready-culture medium system which contains selective agents, nutrients, a cold-water-soluble gelling agent, and an indicator
Component Product # Product # Cell Lysis Reagent 100 ml 500 ml Product Insert 1 1
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cell Lysis Reagent Product # 18800 (100 ml) Product # 18801 (500
Impedimetric evaluation of the synergistic effects of oregano and cranberry aqueous extract mixtures on the growth of Salmonella typhimurium
2013 Scienceweb Publishing Medicinal and Aromatic Plant Research Journal Vol. 1(2), pp. 12-19, December 2013 Research Paper Impedimetric evaluation of the synergistic effects of oregano and cranberry aqueous
Transcription of the SsrAB Regulon Is Repressed by Alkaline ph and Is Independent of PhoPQ and Magnesium Concentration
JOURNAL OF BACTERIOLOGY, Mar. 2002, p. 1493 1497 Vol. 184, No. 5 0021-9193/02/$04.00 0 DOI: 10.1128/JB.184.5.1493 1497.2002 Copyright 2002, American Society for Microbiology. All Rights Reserved. Transcription
Tineke Jones Agriculture and Agri-Food Canada Lacombe Research Centre Lacombe, Alberta
Growth of Escherichia Coli at Chiller Temperatures Tineke Jones Agriculture and Agri-Food Canada Lacombe Research Centre Lacombe, Alberta \ Introduction The responses of mesophilic microorganisms to chiller
The effect of short-time microwave exposures on Salmonella typhimurium inoculated onto chicken drumettes
Short Paper The effect of short-time microwave exposures on Salmonella typhimurium inoculated onto chicken drumettes Jamshidi, A. 1* ; Ghasemi, A. 2 and Mohammadi, A. 3 1 Department of Food Hygiene and
Nitric Oxide Synthase Assay Kit
Nitric Oxide Synthase Assay Kit Catalog Number KA1634 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay...
Evaluation of non-pathogenic surrogate bacteria as process validation indicators
Evaluation of non-pathogenic surrogate bacteria as process validation indicators for Salmonella enteric for selected antimicrobial treatments, cold storage and fermentation in meat S. E. Niebuhr 1, A.
World Journal of Pharmaceutical Research SJIF Impact Factor 8.074
SJIF Impact Factor 8.074 Volume 7, Issue 5, 966-973. Research Article ISSN 2277 7105 MOLECULAR DETECTION OF ENTEROTOXIGENIC ISOLATES OF SALMONELLA TYPHIMURIUM, SHIGELLA FLEXNERI AND STAPHYLOCOCCUS AUREUS
Gram negative bacilli
Gram negative bacilli 1-Enterobacteriaceae Gram negative bacilli-rods Enterobacteriaceae Are everywhere Part of normal flora of humans and most animals They are cause of -30-35% septisemia -more than 70%
Fluoro NADP/NADPH Fluorescent NADP/NADPH Detection Kit
Fluoro NADP/NADPH Fluorescent NADP/NADPH Detection Kit Contact Information Address Telephone Toll Free Fax General Information Sales Technical Questions Website Cell Technology Inc 950 Rengstorff Ave Suite
Monensin ELISA Kit. Catalog Number KA assays Version: 11. Intended for research use only.
Monensin ELISA Kit Catalog Number KA1422 96 assays Version: 11 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General Information...
Optical forward scattering for colony identification and differentiation of bacterial species
Optical forward scattering for colony identification and differentiation of bacterial species Arun K. Bhunia, E. D. Hirleman and J.P. Robinson Center for Food Safety and Engineering, Purdue University
NF VALIDATION Validation of alternative analytical methods Application in food microbiology. Summary report
ACCREDITATION N 1-0144 PORTEE DISPONIBLE SUR WWW.COFRAC.FR THERMO FISHER SCIENTIFIC 2130 Woodward Austin, TX 78744 USA NF VALIDATION Validation of alternative analytical methods Application in food microbiology
PREDICTIVE MICROBIOLOGICAL MODELS: WHAT ARE THEY AND HOW CAN THEY BE USED IN THE FOOD INDUSTRY?
PREDICTIVE MICROBIOLOGICAL MODELS: WHAT ARE THEY AND HOW CAN THEY BE USED IN THE FOOD INDUSTRY? WHY USE MODELS? Predictive microbiological models are tools that can be used to assess product shelf-life
Synergistic Effect of High Temperature and High ph on the Destruction of Salmonella enteritidis and Escherichia co1i0157:h7
1023 Journal of Food Protection, Vol. 5, No. 10, 16, Pages 1023-1030 Copyrighte>, nternational Association of Milk, Food and Environmental Sanitarians Synergistic Effect of High Temperature and High ph
Stephen J. Knabel, Ph.D.
Stephen J. Knabel, Ph.D. Professor of Food Science 437 Food Science Building The Pennsylvania State University, University Park, PA 16802 EDUCATION AND PROFESSIONAL EXPERIENCE July 1, 2006 Present. Professor
Predictive food microbiology
Predictive food microbiology Tina Beck Hansen (tibha@food.dtu.dk) What is it? How is it done? What is it used for? Predictive food microbiology What is it? Prediction of microbial behaviour in food environments
Microbiology. Definition of a Microorganism. Microorganisms in the Lab. The Study of Microorganisms
Microbiology The Study of Microorganisms Definition of a Microorganism Derived from the Greek: Mikros, «small» and Organismos, organism Microscopic organism which is single celled (unicellular) or a mass
Introduction to Bacteria
Introduction to Bacteria USDA NIFSI Food Safety in the Classroom University of Tennessee, Knoxville 2006 A quick clip http://www2.beavercreek.k12.oh.us/vi deos/28824/chp937402_700k.asf Bacteria What are
Dynamics of Salmonella Typhimurium shedding from early to peak lay in laying hens
Dynamics of Salmonella Typhimurium shedding from early to peak lay in laying hens P. SHARMA*, V. PANDE, R. DEVON, A. MCWHORTER and K. K. CHOUSALKAR School of Animal and Veterinary Sciences, University
SUPPLEMENTARY INFORMATION
doi:10.1038/nature11419 Supplementary Figure 1 Schematic representation of innate immune signaling pathways induced by intracellular Salmonella in cultured macrophages. a, During the infection Salmonella
DISSERTATION EVALUATION OF RISK FACTORS AND DETECTION OF SELECTED FOODBORNE PATHOGENS ASSOCIATED WITH FRESH PRODUCE. Submitted by. Shannon M.
DISSERTATION EVALUATION OF RISK FACTORS AND DETECTION OF SELECTED FOODBORNE PATHOGENS ASSOCIATED WITH FRESH PRODUCE Submitted by Shannon M. Coleman Department of Animal Sciences Doctoral Committee: Advisor:
Salmonella in Food Table 1: Salmonella spp. in meat
Salmonella in Food 2013 Table 1: Salmonella spp. in meat Food Type Sampling Site RTE Status Samples Tested Samples Positive % Positive FRESH MEAT BROILER Processing NS 43 0 0 Slaughterhouse NS 7 0 0 BEEF
Know Your Microbes Introduction to food microbiology Factors affecting microbial growth Temperature Time
Know Your Microbes Know Your Microbes Introduction to food microbiology Factors affecting microbial growth Temperature Time ph Water activity (Aw) Nutrient availability Atmosphere Hurdle technology Foodborne
Product List. - Kits & Reagents
Product List - Kits & Reagents Bacterial Pathogens Viral Pathogens Beverage Spoilage Organisms Yeast and Mold GMOs Allergens Animal Identification Veterinary Environmental Pharma DNA/RNA Extraction Additional
NF VALIDATION Validation of alternative analytical methods Application in food microbiology. Summary report
ACCREDITATION N 1-0144 PORTEE DISPONIBLE SUR WWW.COFRAC.FR Boulevard de l Oise F-95029 CERGY PONTOISE CEDEX NF VALIDATION Validation of alternative analytical methods Application in food microbiology Summary
Survival and Heat Resistance of Salmonella enterica and Escherichia coli O157:H7 in Peanut Butter
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Dec. 2011, p. 8434 8438 Vol. 77, No. 23 0099-2240/11/$12.00 doi:10.1128/aem.06270-11 Copyright 2011, American Society for Microbiology. All Rights Reserved. Survival
Cell Review. 1. The diagram below represents levels of organization in living things.
Cell Review 1. The diagram below represents levels of organization in living things. Which term would best represent X? 1) human 2) tissue 3) stomach 4) chloroplast 2. Which statement is not a part of
COMPANY WITH QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL = ISO 9001 = Complement CH50 Assay. Kit Insert
COMPANY WITH QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL = ISO 9001 = Complement CH0 Assay Kit Insert Version: November 01 Summary Complement hemolytic activity is a functional test of the classical
PSI Report Salmonella Testing - Algorithm Tuning - by J. J. Lehett, Director of Engineering & P. J. Simpson, V. P. of Technology Development
PSI Report Salmonella Testing - Algorithm Tuning - by J. J. Lehett, Director of Engineering & P. J. Simpson, V. P. of Technology Development Executive Summary: RV Results: 64 total samples run; 44 positives
Collaborators. Page 1 of 7
Anti-Salmonella and Anti-Campylobacter Properties of Sodium Metasilicate on Commercially Available Ready-to-Cook Broiler Breast Meat Stored at 4 ± 1 C for 7 Days Collaborators Sally K. Williams, Ph.D.
Figure Small ByT-FAS raw data plots of a quinine tracer injected into the sample carrier stream.
Figure 3.23. Small ByT-FAS raw data plots of a quinine tracer injected into the sample carrier stream. Quinine sample was injected into the sample side of the system with different sample injection loop
D-Lactate Fluorometric/Colorimetric Assay Kit
D-Lactate Fluorometric/Colorimetric Assay Kit Catalog No. KM0096 Detection and Quantification of D-Lactate Concentrations in Biological Samples. Research Purposes Only. Not Intended for Diagnostic or Clinical
C. elegans as an in vivo model to decipher microbial virulence. Centre d Immunologie de Marseille-Luminy
C. elegans as an in vivo model to decipher microbial virulence Centre d Immunologie de Marseille-Luminy C. elegans : a model organism Mechanisms of apoptosis, RNA interference Neuronal function and development
June 24, Q Laboratories, Inc Harrison Avenue Cincinnati, OH 45214
June 24, 2011 A Comparative Evaluation of the Aerobic Procedure for Lactic Acid Bacteria with 3M Petrifilm Aerobic Count Plates with Two Reference Methods for the Enumeration of Lactic Acid Bacteria in
Project Title: Estimation of the area affected by animal feces in vegetable field under overhead sprinkle irrigation system
I. Abstract. Project Title: Estimation of the area affected by animal feces in vegetable field under overhead sprinkle irrigation system Project Investigator(s): Jorge M. Fonseca 1, Sadhana Ravishankar
Hospitality Institute of Technology and Management, 670 Transfer Road, Suite 21A, St. Paul, MN 55114, USA;
Food Protection Trends, Vol. 30, No. 4, Pages 223 229 International Association for Food Protection 6200 Aurora Ave., Suite 200W, Des Moines, IA 50322-2864 Controlled Vapor Oven Cooking and Holding Procedures
SUPPORTING INFORMATION
SUPPORTING INFORMATION Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay Zdeněk Farka, 1, * Veronika Čunderlová, 1,2 Veronika Horáčková, 1 Matěj Pastucha,
Novel antibiotics from symbiotic peptides
HU-NO Research conference and Knowledge exchange 15.02.2018 Novel antibiotics from symbiotic peptides Eva Kondorosi Biological Research Centre Hungarian Academy of Sciences Medicago truncatula-sinorhizobium
Salmonella monitoring data and foodborne outbreaks for 2015 in the European Union
Salmonella monitoring data and foodborne outbreaks for 2015 in the European Union Valentina Rizzi BIOCONTAM Unit 22 nd EURL- Salmonella Workshop 2017 Zaandam, 29-30 May 2017 OUTLINE EUSR zoonoses-fbo 2015
Dr. Dina A. A. Hassan Associate Professor, Pharmacology
Cytology Dr. Dina A. A. Hassan Associate Professor, Pharmacology Email: da.hassan@psau.edu.sa Cells All living things are made up of cells Basic building blocks of life It is the smallest functional and
SEL, a Selective Enrichment Broth for Simultaneous Growth of. Listeria monocytogenes
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Aug. 2008, p. 4853 4866 Vol. 74, No. 15 0099-2240/08/$08.00 0 doi:10.1128/aem.02756-07 Copyright 2008, American Society for Microbiology. All Rights Reserved. SEL,
Potential of atmospheric cold plasma for biofilm control in food processing
Potential of atmospheric cold plasma for biofilm control in food processing Paula Bourke, PhD, Dublin Institute of Technology paula.bourke@dit.ie IAFP European Symposium, 30 th March 2017 RATIONALE FOR
Biology. Chapter 2 Notes
Biology Chapter 2 Notes Section 1: Nature of Matter Objectives: 1) Differentiate between atoms and elements 2) Analyze how compounds are formed 3) Distinguish between covalent bonds, hydrogen bonds and
Succinate (Succinic Acid) Assay Kit (Colorimetric)
Succinate (Succinic Acid) Assay Kit (Colorimetric) Catalog Number KA3955 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background...
TrioMol Isolation Reagent
TrioMol Isolation Reagent Technical Manual No. 0242 Version 06142007 I Description... 1 II Key Features... 1 III Storage..... 1 IV General Protocol Using Triomol Isolation Reagent 1 V Troubleshooting.
Pharmaceutical Microbiology Forum Newsletter Vol. 12 (4) Page 3 of 14 (NCIMB 8545, CIP NBRC. Salmonella enterica ssp typhimurium
Page 3 of 14 Continued from page 2 Table 2. Absence of Specified Details Media Growth Promotion Organisms for Trypticase Soy Staphylococcus aureus Escherichia coli Pseudomonas aeruginosa Salmonella Staphylococcus
TrioMol Isolation Reagent
TrioMol Isolation Reagent Technical Manual No. 0242 Version 06142007 I Description... 1 II Key Features... 1 III Storage..... 1 IV General Protocol Using Triomol Isolation Reagent 1 V Troubleshooting.
The effect of salinomycin on Salmonella, Campylobacter and the intestinal microflora in experimentally infected broiler chickens
The effect of salinomycin on Salmonella, Campylobacter and the intestinal microflora in experimentally infected broiler chickens C. H. JOHANSEN, L. BJERRUM, M. LUND and K. PEDERSEN* Danish Institute for
Inhibition of Salmonella Typhimurium and Listeria monocytogenes in Mung Bean Sprouts by Chemical Treatment
1088 Journal of Food Protection, Vol. 65, No. 7, 2002, Pages 1088 1092 Copyright Q, International Association for Food Protection Inhibition of Salmonella Typhimurium and Listeria monocytogenes in Mung
Name: Date: Period: Biology Notes: Biochemistry Directions: Fill this out as we cover the following topics in class
Name: Date: Period: Biology Notes: Biochemistry Directions: Fill this out as we cover the following topics in class Part I. Water Water Basics Polar: part of a molecule is slightly, while another part
NF VALIDATION Validation of alternative analytical methods Application in food microbiology. Summary report
ACCREDITATION N 1-0144 PORTEE DISPONIBLE SUR WWW.COFRAC.FR Boulevard de l Oise F-95029 CERGY PONTOISE CEDEX NF VALIDATION Validation of alternative analytical methods Application in food microbiology Summary
The effects of agar concentration on the growth and morphology of submerged colonies of motile and nonmotile
Journal of Applied Microbiology 1997, 83, 76 8 The effects of agar concentration on the growth and morphology of submerged colonies of motile and nonmotile bacteria A.J. Mitchell and J.W.T. Wimpenny School
Product List. - Kits & Reagents
Product List - Kits & Reagents Bacterial Pathogens Viral Pathogens Beverage Spoilage Organisms Yeast and Mold GMOs Allergens Animal Identification Veterinary Environmental Pharma DNA/RNA Extraction Additional
VOCABULARY. Cell Membrane Nucleus Cell Wall Chloroplast Vacuole Tissue Organ Organ System
CELLS VOCABULARY Cell Membrane Nucleus Cell Wall Chloroplast Vacuole Tissue Organ Organ System THE IMPORTANCE OF CELLS Cells are the smallest unit of life in all living things They are organized structures
cgmp ELISA Kit (Direct Competitive) Based on Monoclonal Anti-cGMP Antibody
(FOR RESEARCH USE ONLY. DO NOT USE IT IN CLINICAL DIAGNOSIS!) cgmp ELISA Kit (Direct Competitive) Based on Monoclonal Anti-cGMP Antibody Catalog No: E-EL-DS02 96T This manual must be read attentively and
Survival of artificially inoculated Escherichia coli and Salmonella Typhimurium on the surface of raw poultry products subjected to crust freezing
Survival of artificially inoculated Escherichia coli and Salmonella Typhimurium on the surface of raw poultry products subjected to crust freezing B. D. Chaves, I. Y. Han, P. L. Dawson, 1 and J. K. Northcutt
Denaturation and renaturation of proteins
Denaturation and renaturation of proteins Higher levels of protein structure are formed without covalent bonds. Therefore, they are not as stable as peptide covalent bonds which make protein primary structure
SULFIDE-INDOLE-MOTILITY (SIM) TEST
Microbiology Laboratory (BIOL 3702L) Page 1 of 5 Principle and Purpose SULFIDE-INDOLE-MOTILITY (SIM) TEST Using Sulfide-Indole-Motility (SIM) media, various Gram-negative enteric bacilli can be distinguished
Cells & Cell Organelles. Doing Life s Work
Cells & Cell Organelles Doing Life s Work Types of cells bacteria cells Prokaryote Eukaryotes animal cells plant cells Cell size comparison Animal cell Bacterial cell most bacteria 1-10 microns eukaryotic
Tetracyclines (TCs) ELISA Test Kit
Tetracyclines (TCs) ELISA Test Kit 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Tetracyclines in the sample. The coupling antigens are pre-coated on the
Malachite Green Phosphate Detection Kit Catalog Number: DY996
Malachite Green Phosphate Detection Kit Catalog Number: DY996 This Malachite Green Phosphate Detection Kit employs a simple, sensitive, reproducible, and non-radioactive method for measuring inorganic
Supplemental Information. Measuring Markers of Liver Function Using a Micro-Patterned Paper Device. Designed for Blood from a Fingerstick
Supplemental Information Measuring Markers of Liver Function Using a Micro-Patterned Paper Device Designed for Blood from a Fingerstick Sarah J. Vella 1, Patrick Beattie 2, Rebecca Cademartiri 1, Anna
The Relative Efficacy of Thermal and Acidification Stresses on the Survival of E. coli O157:H7, Salmonella, and Listeria Monocytogenes in Ground Beef
Journal of Food Research; Vol. 6, No. 3; 2017 ISSN 1927-0887 E-ISSN 1927-0895 Published by Canadian Center of Science and Education The Relative Efficacy of Thermal and Acidification Stresses on the Survival
Richard K. Gast 1 and Peter S. Holt
Influence of the Level and Location of Contamination on the Multiplication of Salmonella enteritidis at Different Storage Temperatures in Experimentally Inoculated Eggs Richard K. Gast 1 and Peter S. Holt
Complete the notes on photosynthesis in the spaces below.
Section: 3.2 Name: Opening Activity: What type of energy is absorbed by pigment molecules in plant cells to start photosynthesis? Latin Root Word: Review of Old Information: ATP then provides the energy
Biology. Mrs. Michaelsen. Types of cells. Cells & Cell Organelles. Cell size comparison. The Cell. Doing Life s Work. Hooke first viewed cork 1600 s
Types of cells bacteria cells Prokaryote - no organelles Cells & Cell Organelles Doing Life s Work Eukaryotes - organelles animal cells plant cells Cell size comparison Animal cell Bacterial cell most
Natural Genetic Resistance to Infection
Natural Genetic Resistance to Infection The Discovery of Natural Determinants of Susceptibility to Infection in Cattle, especially Tarentaise Steve A Carlson, DVM PhD Tim A Day, PhD PSR Genetics, LLC Scott
Contains ribosomes attached to the endoplasmic reticulum. Genetic material consists of linear chromosomes. Diameter of the cell is 1 m
1. (a) Complete each box in the table, which compares a prokaryotic and a eukaryotic cell, with a tick if the statement is correct or a cross if it is incorrect. Prokaryotic cell Eukaryotic cell Contains
Chapter 6 Microbial Growth With a focus on Bacteria
Chapter 6 Microbial Growth With a focus on Bacteria Temperature Minimum growth temperature Optimum growth temperature Maximum growth temperature Usually within a 30-40 degree range Microbial growth = increase
Peroxidase Assay Kit. Catalog Number KA assays Version: 02. Intended for research use only.
Peroxidase Assay Kit Catalog Number KA1620 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
Under the Radar Screen: How Bugs Trick Our Immune Defenses
Under the Radar Screen: How Bugs Trick Our Immune Defenses Session 2: Phagocytosis Marie-Eve Paquet and Gijsbert Grotenbreg Whitehead Institute for Biomedical Research Salmonella Gram negative bacteria
Evaluation of the efficiency of Mxxxx as a barrier against microrganisms crossing
Evaluation of the efficiency of as a barrier against microrganisms crossing A) composition of filter The filter of has the following characteristics: 1. An outer layer, which is composed by a medical,
Tellurite resistance protein/ethidium efflux transporter/ proflavin transporter. Putative inner membrane protein: function unknown
Additional file 1. Table S1 and Figures S1-4 of Zhang et al. High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG Table S1. Properties of the membrane proteins used
Estimation of Microbial Concentration in Food Products from Qualitative Microbiological Test Data with the MPN Technique
173 Original Paper Estimation of Microbial Concentration in Food Products from Qualitative Microbiological Test Data with the MPN Technique Hiroshi Fujikawa* Faculty of Agriculture, Tokyo University of
Experiment 4 - BIOC 221 W2019. The Subject for the should be: BIOCHEM 221 EXP4
EXCEL SHEET INSTRUCTIONS 1. a) On the web page there is a file called YOURNAME_Bioc221Exp4W2019.xlsx. Fill in the information colored RED with your own information. (Please do not readjust where the cells
RayBio Human IDUA ELISA Kit
RayBio Human IDUA ELISA Kit Catalog #: ELH-IDUA User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross, GA
Introductory Microbiology Dr. Hala Al Daghistani
Introductory Microbiology Dr. Hala Al Daghistani Why Study Microbes? Microbiology is the branch of biological sciences concerned with the study of the microbes. 1. Microbes and Man in Sickness and Health