Test Kit Instructions

Transcription

1 Test Kit Instructins QuenchGne21 TM Specialty Test Kit Prduct # QG21S-50 / QG21St-100 At LuminUltra, we are cmmitted t prviding high quality test kits t anyne that needs fast and reliable results abut the micrbilgical characteristics f any prcess! Visit t learn abut all the exciting pprtunities that ur technlgy can bring t yur applicatin. Whereas traditinal micrbilgical tests require days fr feedback and measure nly a fractin f the micrrganisms, 2 nd Generatin Adensine Triphsphate (ATP) test kits frm LuminUltra measure ttal micrrganisms and prvide feedback in minutes! In this test kit instructin guide, yu will learn... Where this kit can be used; Hw 2 nd Generatin ATP technlgy wrks; Hw t handle and stre cmpnents f this kit; Hw t perfrm tests; Hw t calculate and interpret results; and Where t find mre infrmatin and hw t cntact us. Chsing the Right Test Kit LuminUltra prvides 6 cre test kits fr measuring ttal micrbilgical cncentratin via ATP, each tailred t specific applicatins: Quench-Gne TM Aqueus (QGA TM ): Fr lw-slids water-based samples, such as drinking, cling and ilfield waters. Quench-Gne Organic Mdified (QGO-M TM ): Fr lw-slids rganic-based samples, such as fuel, ily brine, lubricants and latex plymers. Depsit & Surface Analysis (DSA TM ): Fr measuring depsits and surfaces, including crrsin prducts and slimes. QuenchGne21 Industrial (QG21I TM ): Fr high-slids prcess fluids, including paper prcess and ther wash waters. QuenchGne21 Specialty (QG21S TM ): Fr chemical prduct testing, such as slurries, adhesives and ther catings. QuenchGne21 Wastewater (QG21W TM ): Fr wastewater and biprcessing samples, whether influent, bireactr r effluent. All test kits are designed t be used with the majrity f phtmultiplier-based luminmeters. New users are advised t acquire the kit r kits f their chice t pair with the Field Kit & Luminmeter Package (Prduct # EQP-PAC) when getting started with LuminUltra s 2 nd Generatin ATP test kits. QG21S Test Kit (QG21S-50C) LuminUltra Technlgies Ltd. 440 King Street, King Twer, Suite 630 Frederictn, New Brunswick, Canada E3B 5H supprt@luminultra.cm LuminUltra 2010 Dcument v1.0 Page 1 f 8

2 Where Can I Use QG21S? QG21S test kits prvide a real-time measurement f ttal micrrganism ppulatin and stress level in high slids slurries and prcess fluids. Use QG21S t detect ttal micrbilgical activity in: Slurries Adhesives Paints Catings and mre! QG21S test kits are available in tw frmats. Chse the best frmat t suit yur needs frm the fllwing descriptins: QG21S Standard (QG21S-50) prvides materials t perfrm 50 analyses each f Ttal ATP (r tatp TM : living plus dead bimass) and Disslved ATP (r datp TM : dead bimass nly). This prvides the mst accurate indicatin f living micrrganisms nly via Cellular ATP and allws cmputatin f the Bimass Stress Index t assess micrbial ppulatin health. QG21S tatp Only (QG21St-100) prvides materials t perfrm nly 100 analyses f tatp. Use this kit when n differentiatin between living and dead micrbes is required. Bimass Stress Index (BSI ) represents the stress level experienced by the micrbilgical ppulatin. QG21S is capable f detecting ATP levels as lw as 20 pg ATP/mL using standard prcedures and equipment. Getting Started LuminUltra s test kits cntain all f the cnsumable materials required t run their specified number f tests (Defined by the last 2 r 3 digits f the prduct cde). T use these test kits, LuminUltra recmmends ne f the fllwing equipment bundles: Field Kit & Luminmeter (EQP-PAC): Field Case, Micrpipettrs, Kikkman Lumitester. Bench Materials & Luminmeter (EQP-BTM): Micrpipettrs, Kikkman Lumitester, Test Racks. Kikkman Lumitester TM C-110 (EQP-LUK): Kikkman Lumitester luminmeter & accessries. Hw Des ATP Testing Wrk? LuminUltra s test kits are based n the measurement f ATP. ATP is a direct and interference-free indicatr f ttal living bimass. ATP is measured using the firefly luciferase assay, where a sample cntaining ATP is intrduced t a slutin cntaining the enzyme Luciferase, which naturally ccurs in the tails f fireflies, t prduce light. The light is detected in a luminmeter as Relative Light Units (). Mg ++ luciferase ATP + O2 + luciferin AMP + PPi + xyluciferin + light The QG21S Standard test kit uses tw parallel 1- minute analyses (tatp and datp) n each sample t determine tw valuable pieces f infrmatin: Cellular ATP (catp ) represents ATP frm living micrrganisms and therefre is a direct indicatin f ttal living micrrganisms. Field Kit & Luminmeter Package (EQP-PAC) NOTE: LuminUltra s test kits can be used with the majrity f phtmultiplier tube based luminmeters. Cntact LuminUltra t cnfirm cmpatibility f yur luminmeter. LuminUltra is sensitive t the needs f each individual custmer. Our expert staff can cnsult with yu t determine the best means by which the data cllected frm ur test kits can be integrated int yur existing data management practices. We have multiple sftware platfrms and can even assist yu t integrate ATP data directly int existing cntrl systems, prcess histrians, and data archives. Getting started with QG21S is easier with direct training. LuminUltra can supply yu with n-site LuminUltra 2010 Page 2 f 8

3 auditing and training services, web-based training, and ne-n-ne cnsultatin t get yur prcess imprvement prgram ff the grund. Cntact us tday t learn mre! Test Kit Cntents and Strage When yu receive yur QG21S test kit, utilize the fllwing guidelines fr material strage. Nte that the presence and quantity f each item listed belw will depend n test kit size and type. QG21S Test Kit Cntents & Strage Cnditins Cmpnent (LuminUltra P/N) Luminase TM XL Drpper (LuXL-5 Luciferase Enzyme Reagent, 5mL UltraCheck TM 1 Drpper (UC1-5 1 ng ATP/mL Standard, 5mL QG21S Extractin s (UL30(21)--50R) UltraLyse 30 21,, 50/rack QG21S Dilutin s (ULuS-8mL-50R) UltraLute /Resin, 8mL, 50/rack QG21S LumiSlve TM s ** (LS-9mL-50R) LumiSlve, 9mL, 50/rack QG21S Pre-Dilutin s (PRE-DIL-50R) UltraLute, 9mL, 50/rack 100 t 1000µL Blue Pipette Tips, 100/rack (DIS-PT1-100R) *** 1 t 5mL Natural Pipette Tips, 50/pk (DIS-PT5-50) *** 12x55mm Test s, 50/pk (DIS-CT12-50) Stre At Shelf Life 4 C* 4 m* 20 C - 20 C - 20 C - * Luminase XL shelf life can be extended t 6 mnths when frzen, r can be left at rm temperature fr as lng as 3 weeks during rutine use. Nte that the Luminase XL supplied in QG21S kits is NOT interchangeable with ther frms f Luminase (i.e. Luminase, Luminase W, and Luminase Lite). ** Materials are included as part f QG21S Standard (QG21S-50) test kit nly. *** Pipette tips supplied in cmplete test kits are cmpatible with mst Fisherbrand and Eppendrf adjustable micrpipettrs. Preparing t Test New t 2 nd Generatin ATP technlgy? Befre getting started, cnsult the training center at fr vide demnstratins, use guidelines, validatin guidelines, and mre! Be certain t allw Luminase XL t reach ambient temperature prir t use! Fr rm temperature (15 t 25 C) strage, n warming is required. Fr refrigeratr (2 t 8 C) strage, let stand at ambient fr at least 1 hur prir t testing. Fr freezer (-10 t -20 C) strage, let stand at ambient fr at least 2 hurs prir t testing. Luminase XL expsure t temperatures between 30 and 40 C shuld be limited t 1-2 hurs. Prlnged expsure will result in accelerated activity lss. Never expse t temperatures > 40 C. Fr mre infrmatin n Luminase XL strage and handling, cnsult the Luminase XL insert. If yu are new t the use f micrpipettrs, cnsult the Micrpipetting Fundamentals training materials n Avid analysis cntaminatin by always using a new pipette tip fr each pipetting step. Avid usage f expired test kit cmpnents. Cntact LuminUltra t replace expired cmpnents. Because ATP and bacteria are present n skin, d nt t tuch the surface f pipette tips. Ensure that all assay tubes are clean inside and utside. D nt mark n assay tubes as this may impact light detectin by the luminmeter. Micrbilgical characteristics f mst samples will begin t change immediately upn cllectin. Analyze samples within 2 hurs f cllectin whenever pssible. LuminUltra 2010 Page 3 f 8

4 If samples cannt be tested within 2 hurs f cllectin, stre refrigerated (2 t 8 C) and test within 24 hurs f cllectin. Ensure that samples are first allwed t reach ambient temperature prir t testing. Perfrm ATP analyses n the same sample used fr measuring ther parameters fr reliable interpretatin. Waste reagent can be discarded as general waste in mst cases. Cnsult MSDS fr mre infrmatin. Obtain MSDS and ther prduct dcumentatin frm Diagnstics and Trubleshting LuminUltra s test kits are the mst rbust and quantitative ATP test kits available. If yu suspect yu are encuntering interferences in yur applicatin f these kits, first check t ensure yu are using the crrect kit type fr yur sample. If yu are unsure r feel yu have unique testing requirements, cntact LuminUltra s supprt staff fr assistance. Fluid samples with high slids cntent r cmplex nature (e.g. paints, catings, slurries, adhesives) may require pre-dilutin prir t analysis t ensure reliable quantificatin f ATP. The QG21S test kit is designed t prvide the necessary dilutin t vercme such interferences. If yu feel yu may require further prcedure mdificatin, cntact LuminUltra fr additinal recmmendatins n predilutin r sample pre-treatment prir t ATP measurement. When testing samples that yield lw values (i.e. tatp r datp 50 when using a Kikkman Lumitester C-100 r C-110), it is recmmended that yu accunt fr backgrund nise in the test prcedure prir t the final calculatins. T assess backgrund nise, simply fllw the prcedure withut adding any f the ATP-cntaining sample int the analysis. Crrect fr backgrund nise by subtracting the backgrund ( bg ) frm the measured ( tatp r datp ) prir t executing calculatins. Typical bg when using a Kikkman Lumitester C-100 r C-110 are 10. If high bg are cnsistently bserved, repeat assays in an area ut f direct sunlight r intense lighting. If prblems still ccur, cntact LuminUltra fr assistance. A single bg may be used fr multiple analyses much like a single UltraCheck 1 ( UC1 ). If drpper bttles becme plugged r yu encunter difficulty dispensing drps, remve and discard the drpper tip and use a pipettr t measure and dispense the reagent. Step 1 UltraCheck 1 Calibratin Included in QG21S and QG21St test kits. The UltraCheck 1 (UC1) Calibratin cnverts luminmeter values int actual ATP cncentratins. Perfrm ne UltraCheck 1 calibratin per day r fr each set f samples analyzed at the same time. Be sure that Luminase XL is allwed t reach ambient temperature prir t use. PROCEDURE: Add 2 drps (100µL) f UltraCheck 1 and 2 drps (100µL) f Luminase XL t a new 12x55mm test tube (the Assay ), swirl gently five times, immediately insert int the luminmeter and measure. Recrd UC1 fr use in Step 4. NOTE: If UC1 50,000 using a Kikkman Lumitester C- 100 r C-110, it is recmmended t btain a new bttle f Luminase XL fr maximum sensitivity. NOTE: UC1 will fall ver time fr the same batch f Luminase XL. This is because f decreased luciferase enzyme activity. When fllwed, the guideline abve ensures that there is sufficient activity t meet the specified detectin limit. LuminUltra 2010 Page 4 f 8

5 Step 2 tatp Analysis Included in QG21S and QG21St test kits. The Ttal ATP (tatp) analysis measures ATP frm bth living and dead cells. Perfrm ne tatp analysis n each sample yu wish t test. 2.2 EXTRACTION Using a new wide-bre pipette tip, add f wellmixed, pre-diluted sample t a new QG21S Extractin. Cap and invert three times t mix. Allw at least 1 minute fr incubatin. 2.1 PRE-DILUTION Chemical prducts that are highly turbid are recmmended t be pre-diluted prir t carrying ut the tatp analysis. Because it is imprtant t ensure that the dilutin medium used is free f ATP and ther micrbial cntaminants, LuminUltra prvides predispensed pre-dilutin tubes. Use a new wide-bre pipette tip t transfer f sample int a new QG21S Pre-Dilutin. Mix and immediately prceed t Step 2.2. NOTE: At this pint, the cntents f the QG21S Extractin can be capped and stred refrigerated between 2-8 C fr up t 1 week prir t 2.3. TIP: Wide-bre pipette tips are thse that have sufficiently large penings t prevent tip plugging by sample particles. In general, standard 1-5mL and wide-muth µL pipette tips are sufficient fr mst samples. If required, increase the bre size f a tip using a clean pair f scissrs. 2.3 DILUTION NOTE: Wide-bre pipette tips are thse that have sufficiently large penings t prevent tip plugging by sample particles. In general, standard 1-5mL and wide-muth µL pipette tips are sufficient fr mst samples. If required, increase the bre size f a tip using a clean pair f scissrs. TIP: If a different dilutin is desired, increase r decrease the prprtin f sample t dilutin medium used in this step and adjust the dilutin factr in the final calculatins accrdingly. Fr example, a 1:9 dilutin as shwn abve prvides a dilutin factr f 10, whereas a 1:19 dilutin prvides a dilutin factr f 20. Be aware hwever that t much dilutin will cmprmise test methd sensitivity. Pur the QG21S Extractin cntents int a new QG21S Dilutin. Transfer the mixture back and frth between the tw tubes at least three times fr best mixing accuracy. Cap and invert three times t mix. Allw beads t settle. NOTE: At this pint, the cntents f the QG21S Dilutin are stable at rm temperature fr up t 4 hurs. TIP: If beads d nt settle r settle slwly, tap the QG21S Dilutin gently t assist settling. TIP: If the QG21S Extractin cannt be pured int the QG21S Dilutin, simply pur the QG21S Dilutin cntents int the QG21S Extractin t liquefy its cntents. LuminUltra 2010 Page 5 f 8

6 2.4 ASSAY Using a new pipette tip, add 100µL f the QG21S Dilutin cntents t a new 12x55mm test tube (the Assay ), add 2 drps (100µL) f Luminase XL, swirl gently five times, immediately insert int the luminmeter and measure. Recrd tatp fr use in Step 4. NOTE: If tatp 10 n a Kikkman Lumitester C-100 r C-110, yu are belw the lw detectin limit. Reprt tatp (pg ATP/ = 0 in Step 4. NOTE: When tatp 50 n a Kikkman Lumitester C-100 r C-110, it is recmmended that yu measure and subtract bg frm yur measurement. See Diagnstics and Trubleshting. TIP: If Scale Over is returned, repeat the tatp analysis using 100µL f pre-diluted sample in 2.2 (EXTRACTION) and mdify the dilutin factr in Step 4 as nted. Step 3 datp Analysis Included in QG21S Standard test kit nly. The Disslved ATP (datp) analysis measures ATP frm nly dead cells. Perfrm ne datp analysis n each sample yu wish t test. NOTE: Use the same pre-diluted sample as prepared in Step 2.1 fr the tatp analysis when using the datp analysis. TIP: Wide-bre pipette tips are thse that have sufficiently large penings t prevent tip plugging by sample particles. In general, standard 1-5mL and wide-muth µL pipette tips are sufficient fr mst samples. If required, increase the bre size f a tip using a clean pair f scissrs. 3.2 ASSAY Using a new pipette tip, add 100µL f the QG21S LumiSlve cntents t a new 12x55mm test tube (the Assay ), add 2 drps (100µL) f Luminase XL, swirl gently five times, immediately insert int the luminmeter and measure. Recrd datp fr use in Step 4. NOTE: If datp 10 n a Kikkman Lumitester C-100 r C-110, yu are belw the lw detectin limit. Reprt datp (pg ATP/ = 0 in Step 4. NOTE: When datp 50 n a Kikkman Lumitester C-100 r C-110, it is recmmended that yu measure and subtract bg frm yur measurement. See Diagnstics and Trubleshting. TIP: If Scale Over is returned, repeat the datp analysis using 100µL f pre-diluted sample in 3.1 (DILUTION) and mdify the dilutin factr in Step 4 as nted. 3.1 DILUTION Using a new wide-bre pipette tip, add f wellmixed, pre-diluted sample t a QG21S LumiSlve. Cap and invert three times t mix. Allw at least 1 minute fr incubatin. NOTE: The QG21S LumiSlve can be capped and stred between 2 t 8 C fr up t 1 week prir t LuminUltra 2010 Page 6 f 8

7 Step 4 Intermediate Calculatins Fllwing cmpletin f QG21S analyses, values must be cnverted t ATP cncentratins using the fllwing calculatins. Fr easy calculatins, utilize LumiCapture TM sftware available n Fr manual calculatins, see belw. 1. Ttal ATP (tatp) measures all ATP within a sample, including ATP frm living cells in additin t ATP that has been released frm dead cells. NOTE: If 100µL f sample was used in 2.2 f the tatp analysis, replace the dilutin factr 100,000 with 1,000,000. tatp ( pg ATP/ = tatp UC1 100,000 ( pg ATP/ NOTE: When applicable, subtract bg frm tatp prir t executing the abve calculatin. 2. Disslved ATP (datp) represents ATP within a sample that has been released frm dead cells. NOTE: If 100µL f sample was used in 3.1 f the datp analysis, replace the dilutin factr 100,000 with 1,000,000. datp ( pg ATP/ = datp UC1 100,000 ( pg ATP/ NOTE: When applicable, subtract bg frm datp prir t executing the abve calculatin. Use these results t perfrm the final calculatins shwn in the next sectin. NOTE: When using the QG21S tatp-nly kit, skip the final calculatins and interpret tatp results as yu wuld catp results in the Interpretatin Guidelines. Final Calculatins Fr mnitring basic bimass cncentratin and health at any prcess lcatin, the fllwing 2 parameters are used. Fr easy calculatins, use LumiCapture sftware, which can be btained at Fr manual calculatins, see belw. NOTE: If the results f Step 4 shw fr a given sample that datp (ng/ > tatp (ng/, bypass these calculatins and reprt catp = 0 and BSI = 100%. 1. Cellular ATP (catp) represents the amunt f ATP cntained within living cells and is a direct indicatin f ttal living bimass (quantity). catp ( pg ATP/ = tatp ( pg ATP/ datp ( pg ATP/ T cmmunicate results n the same basis as traditinal culture tests, catp results are cnverted int Micrbial Equivalents (ME s). This is based n the established cnversin that 1 E. cli-sized bacteria cntains pg (1 fg) f ATP. catp ( ME = catp ( pg ATP / / 1ME pg ATP NOTE: Fr mre discussin n the quantity f ATP per cell, visit Because many f the traditinal culture-based methds reprt results in a similar fashin, it is smetimes cnvenient t reprt catp results in ME/mL using Scientific Ntatin (i.e. #.# x 10 # ) r n a Lg 10 frmat fr cmparisn purpses. 2. Bimass Stress Index (BSI) prvides a measure f the stress level (quality) f the micrbilgical cmmunity. datp ( pg ATP/ BSI (%) = tatp ( pg ATP/ Interpretatin Guidelines 100% Once QG21S catp and BSI results are calculated, micrbial cntrl can be evaluated. ATP-based measurements are extremely sensitive t changes in ttal micrbial quantity. In general, prcesses will have the best micrbial cntrl when catp is minimized and BSI is maximized. LuminUltra s ATP test kits can be used t audit micrbial quantity t reveal differences at different prcess lcatins in an effrt t quickly assess the ht spts within a prcess that require mre immediate attentin. Fr prcess cntrl, daily mnitring using ATP test kits will give yu true ttal micrbial quantity parameters t trend ver time against prcess characteristics and perfrmance. When utilizing ATP test kits it is imprtant t remember that every prcess is different. During audits, relative LuminUltra 2010 Page 7 f 8

8 cmparisns frm pint t pint are a reliable means t assess yur prcess, while fr daily mnitring it is imprtant t establish a baseline trend befre making cntrl decisins. T get started, LuminUltra prvides the fllwing catp guidelines in units f pg catp per ml: Applicatin QG21S catp Interpretatin Guidelines Prduct Quality Cntrl (Paints, Catings, Slurries) Gd Cntrl (pg catp/ < 100 Preventive Actin (pg catp/ 100 t 1,000 Crrective Actin (pg catp/ > 1,000 Fr BSI (when applicable), it can generally be interpreted that gd cntrl is achieved at levels f 75% r abve. Preventive actin shuld be taken at levels between 50% and 75%, and crrective actin shuld be taken at levels belw 50%. NOTE: These interpretatin guidelines are designed fr generic risk management guidance nly. Users are encuraged t establish their wn cntrl ranges n which t base prcess decisins. LuminUltra and its affiliates d nt accept any liability fr any decisin r assessment taken r made as a cnsequence f using this test kit. T btain pricing infrmatin, inquire abut ther prducts and services, r t place an rder, cntact LuminUltra r yur authrized representative. LuminUltra Technlgies Ltd. 440 King Street King Twer, Suite 630 Frederictn, New Brunswick Canada E3B 5H8 Tel: Fax: sales@luminultra.cm Majr credit cards (Visa, MasterCard, AMEX) are accepted. Cntact LuminUltra by phne t place credit card rders. Orders generally ship within 3 business days. Yu will receive rder cnfirmatin via Fax r . Ordering Infrmatin New t 2 nd generatin ATP technlgy? Start by rdering the Field Kit & Luminmeter Package (Prduct # EQP-PAC) and the test kit(s) f yur chice. When rerdering materials fr testing, it is preferred t rder cmplete kits. QG21S is available in fur frmats: Descriptin Part # QG21S Standard, 50 Tests, Cmplete * QG21S Standard, 50 Tests, Reagents Only QG21S tatp Only, 100 Tests, Cmplete * QG21S tatp Only, 100 Tests, Reagents Only QG21S-50C QG21S-50 QG21St-100C QG21St-100 * Cmplete kits include LuminUltra-manufactured reagents plus all cnsumables (tips, tubes, filters, syringes) required t run analysis. If yu supply yur wn cnsumables, reagent nly kits are available. Lumitester is a trademark f Kikkman Crpratin, all ther trademarks are the prperty f LuminUltra Technlgies Ltd. LuminUltra 2010 Page 8 f 8

9 Quick-Reference Guide QuenchGne21 TM Specialty Test Kit Prduct # : QG21S-50 / QG21St-100 NOTE: Please refer t Test Kit Instructins during first prduct use and fr additinal details including legal statements. Step 1 UltraCheck TM 1 Calibratin Perfrm ne UltraCheck 1 calibratin per day r per each set f samples analyzed. Luminase XL UltraCheck 1 2 Drps (100µL) Assay 2 Drps (100µL) NOTE: If UC1 50,000 using a Kikkman Lumitester TM C-100 r C-110, btain a new bttle f Luminase. Step 2 tatp TM Analysis (1 per sample) Included in QG21S TM and QG21St test kits. 2.1 PRE-DILUTION Pre-dilute sample. 1 ml 2.3 DILUTION Dilute ut interferences. Step 3 datp TM Analysis (1 per sample) Included in QG21S test kit nly. 3.1 DILUTION Add pre-diluted sample t recver ATP. Final Calculatins NOTE: When using the QG21S tatp-nly kit, skip final calculatins and interpret tatp results as yu wuld catp results using the Interpretatin Guidelines. NOTE: If the results shw fr a given sample that datp (ng/ > tatp (ng/, reprt catp TM = 0 and BSI TM = 100%. Sample QG21S Pre-Dilutin QG21S Extractin QG21S Dilutin QG21S Pre-Dilutin QG21S LumiSlve minute catp ( pg / = tatp (pg ATP / datp (pg ATP / 1ME catp ( ME / = catp ( pg ATP / pg ATP 2.2 EXTRACTION Add pre-diluted sample t extract ATP. QG21S Pre-Dilutin QG21S Extractin minute 2.4 ASSAY Measure ATP cncentratin. tatp 100 µl 100µL QG21S Dilutin Luminase XL 2 Drps (100µL) Assay NOTE: If tatp 10 n a Kikkman Lumitester C-100 r C-110, yu are belw the lw detectin limit. NOTE: If tatp 50 n a Kikkman Lumitester C-100 r C-110, cnsider accunting fr bg. See Test Kit Instructins. tatp ( pg ATP / = 100,000 ( pg ATP / UC1 3.2 ASSAY Measure ATP cncentratin. 100 µl 100µL QG21S LumiSlve Luminase XL Assay 2 Drps (100µL) NOTE: If datp 10 n a Kikkman Lumitester C-100 r C-110, yu are belw the lw detectin limit. NOTE: If datp 50 n a Kikkman Lumitester C-100 r C-110, cnsider accunting fr bg. See Test Kit Instructins. datp datp ( pg ATP / = 100,000 ( pg ATP / UC1 NOTE: 1 ME (Micrbial Equivalent) assumes pg (1 fg) ATP per cell. datp ( pg ATP/ BSI (%) = 100 % tatp ( pg ATP/ Interpretatin Guidelines Applicatin Prduct Quality Cntrl (Paints, Catings, Slurries) Gd Cntrl (pg catp/ < 100 Preventative Actin (pg catp/ 100 t 1000 Crrective Actin (pg catp/ > 1000 Fr BSI (when applicable), it can generally be interpreted that gd cntrl is achieved at levels f 75% r abve. Preventive actin shuld be taken at levels between 50% and 75%, and crrective actin shuld be taken at levels belw 50%. NOTE: Interpretatin Guidelines are prvided fr general guidance. Fr best results, establish yur wn baseline and cntrl levels. Dcument v1.0 Visit t check fr updates LuminUltra Technlgies Ltd. 2010