Test Kit Instructions

Transcription

1 Test Kit Instructins QuenchGne21 TM Specialty Test Kit QG21S/QG21St Test Kit At Aqua-tls, we are cmmitted t prviding high quality test kits t anyne that needs fast and reliable results abut the micrbilgical characteristics f any prcess! These prducts are made in Canada by LuminUltra. Visit and t learn abut all the exciting pprtunities that ur technlgy can bring t yur applicatin. Whereas traditinal micrbilgical tests require days fr feedback and measure nly a fractin f the micrrganisms, 2 nd Generatin Adensine Triphsphate (ATP) test kits frm Aqua-tls measure ttal micrrganisms and prvide feedback in minutes! In this test kit instructin guide, yu will learn... Where this kit can be used; Hw 2 nd Generatin ATP technlgy wrks; Hw t handle and stre cmpnents f this kit; Hw t perfrm tests; Hw t calculate and interpret results; and Where t find mre infrmatin and hw t cntact us. Chsing the Right Test Kit Aqua-tls prvides 6 cre test kits fr measuring ttal micrbilgical cncentratin via ATP, each tailred t specific applicatins: Quench-Gne TM Aqueus (QGA TM ): Fr lw-slids water-based samples, such as drinking, cling and ilfield waters. Quench-Gne Organic Mdified (QGO-M TM ): Fr lw-slids rganic-based samples, such as fuel, ily brine, lubricants and latex plymers. Depsit & Surface Analysis (DSA TM ): Fr measuring depsits and surfaces, including crrsin prducts and slimes. QuenchGne21 Industrial (QG21I TM ): Fr high-slids prcess fluids, including paper prcess and ther wash waters. QuenchGne21 Specialty (QG21S TM ): Fr chemical prduct testing, such as slurries, adhesives and ther catings. QuenchGne21 Wastewater (QG21W TM ): Fr wastewater and biprcessing samples, whether influent, bireactr r effluent. All test kits are ptimized fr use with the Kikkman Lumitester C-110 Lumitester, althugh mst any phtmultiplier-based luminmeter may be used. New users are advised t acquire the kit r kits f their chice t pair with the Starter kit and Luminmeter when getting started with 2 nd Generatin ATP test kits. QG21S Test Kit Aqua-tls Rute de Renault FLINS-SUR-SEINE +33 (0) cntact@aqua-tls.cm Aqua-tls 2010 Dcument v1.0 Page 1 f 7

2 Test Kit Instructins QuenchGne21 Specialty (QG21S) Where Can I Use QG21S? QG21S test kits prvide a real-time measurement f ttal micrrganism ppulatin and stress level in high slids slurries and prcess fluids. Use QG21S t detect ttal micrbilgical activity in: Slurries Adhesives Paints Catings and mre! QG21S test kits are available in tw frmats. Chse the best frmat t suit yur needs frm the fllwing descriptins: QG21S Standard (QG21S) prvides materials t perfrm 50 analyses each f Ttal ATP (r tatp TM : living plus dead bimass) and Disslved ATP (r datp TM : dead bimass nly). This prvides the mst accurate indicatin f living micrrganisms nly via Cellular ATP and allws cmputatin f the Bimass Stress Index t assess micrbial ppulatin health. QG21S is capable f detecting ATP levels as lw as 20 pg ATP/mL using standard prcedures and equipment. Getting Started Aqua-tls test kits cntain all f the reagents required t run their specified number f tests (defined by the last 2 r 3 digits f the prduct cde). T use these test kits, Aqua-tls recmmends the fllwing equipment and cnsumables: Starter Kit: Micrpipettrs and Tips, Test Tube Racks, Luminmeter Tubes, Extractin Tubes. Kikkman Lumitester C-110: Kikkman Lumitester C-110 luminmeter & accessries. Field Case (nly n specific request): Strng Case t pack Kits and Luminmeter. QG21S tatp Only (QG21St) prvides materials t perfrm nly 100 analyses f tatp. Use this kit when n differentiatin between living and dead micrbes is required. Hw Des ATP Testing Wrk? Aqua-tls test kits are based n the measurement f ATP. ATP is a direct and interference-free indicatr f ttal living bimass. ATP is measured using the firefly luciferase assay, where a sample cntaining ATP is intrduced t a slutin cntaining the enzyme Luciferase, which naturally ccurs in the tails f fireflies, t prduce light. The light is detected in a luminmeter as Relative Light Units (). Mg ++ luciferase ATP + O2 + luciferin AMP + PPi + xyluciferin + light The QG21S Standard test kit uses tw parallel 1- minute analyses (tatp and datp) n each sample t determine tw valuable pieces f infrmatin: Cellular ATP (catp ) represents ATP frm living micrrganisms and therefre is a direct indicatin f ttal living micrrganisms. Bimass Stress Index (BSI ) represents the stress level experienced by the micrbilgical ppulatin. Field Case and Kikkman Lumitester C-110 NOTE: Althugh ptimized fr the Kikkman Lumitester C- 110, Aqua-tls test kits can be used with the majrity f phtmultiplier tube based luminmeters. Cntact Aquatls t cnfirm cmpatibility f yur luminmeter. Aqua-tls is sensitive t the needs f each individual custmer. Our expert staff can cnsult with yu t determine the best means by which the data cllected frm ur test kits can be integrated int yur existing data management practices. We have multiple sftware platfrms and can even assist yu t integrate ATP data directly int existing cntrl systems, prcess histrians, and data archives. Getting started with QG21S is easier with direct training. Aqua-tls can supply yu with n-site auditing and training services, web-based training, and ne-n-ne cnsultatin t get yur prcess imprvement prgram ff the grund. Cntact us tday t learn mre! Aqua-tls 2010 Page 2 f 7

3 Test Kit Instructins QuenchGne21 Specialty (QG21S) Test Kit Cntents and Strage When yu receive yur QG21S test kit, utilize the fllwing guidelines fr material strage. Nte that the presence and quantity f each item listed belw will depend n test kit size and type. QG21S Test Kit Cntents & Strage Cnditins Cmpnent Luminase TM XL Drpper * Luciferase Enzyme Reagent, 5mL UltraCheck TM 1 Drpper 1 ng ATP/mL Standard, 5mL QG21S Extractin Tubes UltraLyse 30 21, 1mL, 50/rack QG21S Dilutin Tubes UltraLute /Resin, 8mL, 50/rack QG21S LumiSlve TM Tubes ** LumiSlve, 9mL, 50/rack QG21S Pre-Dilutin Tubes UltraLute, 9mL, 50/rack Stre At Shelf Life 4 C* 4 m* 20 C 12 m 20 C 12 m 20 C 12 m 20 C 12 m 20 C 12 m * Luminase XL shelf life can be extended t 6 mnths when frzen, r can be left at rm temperature fr as lng as 3 weeks during rutine use. Nte that the Luminase XL supplied in QG21S kits is NOT interchangeable with ther frms f Luminase (i.e. Luminase, Luminase W, and Luminase Lite). ** Materials are nt included in QG21St test kit. Preparing t Test New t 2 nd Generatin ATP technlgy? Befre getting started, cnsult the training center at fr vide demnstratins, use guidelines, validatin guidelines, and mre! Be certain t allw Luminase XL t reach ambient temperature prir t use! Fr rm temperature (15 t 25 C) strage, n warming is required. t 1-2 hurs. Prlnged expsure will result in accelerated activity lss. Never expse t temperatures > 40 C. Fr mre infrmatin n Luminase XL strage and handling, cnsult the Luminase XL insert. If yu are new t the use f micrpipettrs, cnsult the Micrpipetting Fundamentals training materials n Avid analysis cntaminatin by always using a new pipette tip fr each pipetting step. Avid usage f expired test kit cmpnents. Cntact Aqua-tls t replace expired cmpnents. Because ATP and bacteria are present n skin, d nt t tuch the surface f pipette tips. Ensure that all assay tubes are clean inside and utside. D nt mark n assay tubes as this may impact light detectin by the luminmeter. Micrbilgical characteristics f mst samples will begin t change immediately upn cllectin. Analyze samples within 2 hurs f cllectin whenever pssible. If samples cannt be tested within 2 hurs f cllectin, stre refrigerated (2 t 8 C) and test within 24 hurs f cllectin. Ensure that samples are first allwed t reach ambient temperature prir t testing. Perfrm ATP analyses n the same sample used fr measuring ther parameters fr reliable interpretatin. Waste reagent can be discarded as general waste in mst cases. Cnsult MSDS fr mre infrmatin. Obtain MSDS and ther prduct dcumentatin frm Fr refrigeratr (2 t 8 C) strage, let stand at ambient fr at least 1 hur prir t testing. Fr freezer (-10 t -20 C) strage, let stand at ambient fr at least 2 hurs prir t testing. Luminase XL expsure t temperatures between 30 and 40 C shuld be limited Aqua-tls 2010 Page 3 f 7

4 Test Kit Instructins QuenchGne21 Specialty (QG21S) Diagnstics and Trubleshting Aqua-tls test kits are the mst rbust and quantitative ATP test kits available. If yu suspect yu are encuntering interferences in yur applicatin f these kits, first check t ensure yu are using the crrect kit type fr yur sample. If yu are unsure r feel yu have unique testing requirements, cntact Aqua-tls supprt staff fr assistance. Fluid samples with high slids cntent r cmplex nature (e.g. paints, catings, slurries, adhesives) may require pre-dilutin prir t analysis t ensure reliable quantificatin f ATP. The QG21S test kit is designed t prvide the necessary dilutin t vercme such interferences. If yu feel yu may require further prcedure mdificatin, cntact Aqua-tls fr additinal recmmendatins n pre-dilutin r sample pre-treatment prir t ATP measurement. When testing samples that yield lw values (i.e. tatp r datp 50 when using a Kikkman Lumitester C-100 r C-110), it is recmmended that yu accunt fr backgrund nise in the test prcedure prir t the final calculatins. T assess backgrund nise, simply fllw the prcedure withut adding any f the ATP-cntaining sample int the analysis. Crrect fr backgrund nise by subtracting the backgrund ( bg ) frm the measured ( tatp r datp ) prir t executing calculatins. Typical bg when using a Kikkman Lumitester C-100 r C-110 are 10. If high bg are cnsistently bserved, repeat assays in an area ut f direct sunlight r intense lighting. If prblems still ccur, cntact Aqua-tls fr assistance. A single bg may be used fr multiple analyses much like a single UltraCheck 1 ( UC1 ). If drpper bttles becme plugged r yu encunter difficulty dispensing drps, remve and discard the drpper tip and use a pipettr t measure and dispense the reagent. Step 1 UltraCheck 1 Calibratin Included in QG21S and QG21St test kits. The UltraCheck 1 (UC1) Calibratin cnverts luminmeter values int actual ATP cncentratins. Perfrm ne UltraCheck 1 calibratin per day r fr each set f samples analyzed at the same time. Be sure that Luminase XL is allwed t reach ambient temperature prir t use. PROCEDURE: Add 2 drps (100µL) f UltraCheck 1 and 2 drps (100µL) f Luminase XL t a new 12x55mm test tube (the Assay Tube), swirl gently five times, immediately insert int the luminmeter and measure. Recrd UC1 fr use in Step 4. NOTE: If UC1 50,000 using a Kikkman Lumitester C- 100 r C-110, it is recmmended t btain a new bttle f Luminase XL fr maximum sensitivity. NOTE: UC1 will fall ver time fr the same batch f Luminase XL. This is because f decreased luciferase enzyme activity. When fllwed, the guideline abve ensures that there is sufficient activity t meet the specified detectin limit. Step 2 tatp Analysis Included in QG21S and QG21St test kits. The Ttal ATP (tatp) analysis measures ATP frm bth living and dead cells. Perfrm ne tatp analysis n each sample yu wish t test. 2.1 PRE-DILUTION Chemical prducts that are highly turbid are recmmended t be pre-diluted prir t carrying ut the tatp analysis. Because it is imprtant t ensure that the dilutin medium used is free f ATP and ther micrbial cntaminants, Aqua-tls prvides pre-dispensed pre-dilutin tubes. Use a new wide-bre pipette tip t transfer 1mL f sample int a new QG21S Pre-Dilutin Tube. Mix and immediately prceed t Step Aqua-tls 2010 Page 4 f 7

5 Test Kit Instructins QuenchGne21 Specialty (QG21S) NOTE: At this pint, the cntents f the QG21S Dilutin Tube are stable at rm temperature fr up t 4 hurs. TIP: If beads d nt settle r settle slwly, tap the QG21S Dilutin Tube gently t assist settling. NOTE: Wide-bre pipette tips are thse that have sufficiently large penings t prevent tip plugging by sample particles. In general, standard 1-5mL and widemuth µL pipette tips are sufficient fr mst samples. If required, increase the bre size f a tip using a clean pair f scissrs. TIP: If a different dilutin is desired, increase r decrease the prprtin f sample t dilutin medium used in this step and adjust the dilutin factr in the final calculatins accrdingly. Fr example, a 1:9 dilutin as shwn abve prvides a dilutin factr f 10, whereas a 1:19 dilutin prvides a dilutin factr f 20. Be aware hwever that t much dilutin will cmprmise test methd sensitivity. TIP: If the QG21S Extractin Tube cannt be pured int the QG21S Dilutin Tube, simply pur the QG21S Dilutin Tube cntents int the QG21S Extractin Tube t liquefy its cntents. 2.4 ASSAY Using a new pipette tip, add 100µL f the QG21S Dilutin Tube cntents t a new 12x55mm test tube (the Assay Tube), add 2 drps (100µL) f Luminase XL, swirl gently five times, immediately insert int the luminmeter and measure. Recrd tatp fr use in Step EXTRACTION Using a new wide-bre pipette tip, add 1mL f wellmixed, pre-diluted sample t a new QG21S Extractin Tube. Cap and invert three times t mix. Allw at least 1 minute fr incubatin. NOTE: If tatp 10 n a Kikkman Lumitester C-100 r C-110, yu are belw the lw detectin limit. Reprt tatp (pg ATP/ = 0 in Step 4. NOTE: When tatp 50 n a Kikkman Lumitester C- 100 r C-110, it is recmmended that yu measure and subtract bg frm yur measurement. See Diagnstics and Trubleshting. NOTE: At this pint, the cntents f the QG21S Extractin Tube can be capped and stred refrigerated between 2-8 C fr up t 1 week prir t 2.3. TIP: Wide-bre pipette tips are thse that have sufficiently large penings t prevent tip plugging by sample particles. In general, standard 1-5mL and wide-muth µL pipette tips are sufficient fr mst samples. If required, increase the bre size f a tip using a clean pair f scissrs. 2.3 DILUTION Pur the QG21S Extractin Tube cntents int a new QG21S Dilutin Tube. Transfer the mixture back and frth between the tw tubes at least three times fr best mixing accuracy. Cap and invert three times t mix. Allw beads t settle. TIP: If Scale Over is returned, repeat the tatp analysis using 100µL f pre-diluted sample in 2.2 (EXTRACTION) and mdify the dilutin factr in Step 4 as nted. Step 3 datp Analysis Included in QG21S Standard test kit nly. The Disslved ATP (datp) analysis measures ATP frm nly dead cells. Perfrm ne datp analysis n each sample yu wish t test. NOTE: Use the same pre-diluted sample as prepared in Step 2.1 fr the tatp analysis when using the datp analysis. 3.1 DILUTION Using a new wide-bre pipette tip, add 1mL f wellmixed, pre-diluted sample t a QG21S LumiSlve Tube. Cap and invert three times t mix. Allw at least 1 minute fr incubatin. Aqua-tls 2010 Page 5 f 7

6 Test Kit Instructins QuenchGne21 Specialty (QG21S) NOTE: If 100µL f sample was used in 2.2 f the tatp analysis, replace the dilutin factr 100,000 with 1,000,000. tatp ( pg ATP/ = tatp UC1 100,000 ( pg ATP / NOTE: The QG21S LumiSlve Tube can be capped and stred between 2 t 8 C fr up t 1 week prir t 3.2. TIP: Wide-bre pipette tips are thse that have sufficiently large penings t prevent tip plugging by sample particles. In general, standard 1-5mL and wide-muth µL pipette tips are sufficient fr mst samples. If required, increase the bre size f a tip using a clean pair f scissrs. NOTE: When applicable, subtract bg frm tatp prir t executing the abve calculatin. 2. Disslved ATP (datp) represents ATP within a sample that has been released frm dead cells. NOTE: If 100µL f sample was used in 3.1 f the datp analysis, replace the dilutin factr 100,000 with 1,000, ASSAY datp ( pg ATP/ = datp UC1 100,000 ( pg ATP / Using a new pipette tip, add 100µL f the QG21S LumiSlve Tube cntents t a new 12x55mm test tube (the Assay Tube), add 2 drps (100µL) f Luminase XL, swirl gently five times, immediately insert int the luminmeter and measure. Recrd datp fr use in Step 4. NOTE: When applicable, subtract bg frm datp prir t executing the abve calculatin. Use these results t perfrm the final calculatins shwn in the next sectin. NOTE: When using the QG21S tatp-nly kit, skip the final calculatins and interpret tatp results as yu wuld catp results in the Interpretatin Guidelines. Final Calculatins NOTE: If datp 10 n a Kikkman Lumitester C-100 r C-110, yu are belw the lw detectin limit. Reprt datp (pg ATP/ = 0 in Step 4. NOTE: When datp 50 n a Kikkman Lumitester C- 100 r C-110, it is recmmended that yu measure and subtract bg frm yur measurement. See Diagnstics and Trubleshting. TIP: If Scale Over is returned, repeat the datp analysis using 100µL f pre-diluted sample in 3.1 (DILUTION) and mdify the dilutin factr in Step 4 as nted. Fr mnitring basic bimass cncentratin and health at any prcess lcatin, the fllwing 2 parameters are used. Fr easy calculatins, use LumiCapture sftware, which can be btained at Fr manual calculatins, see belw. NOTE: If the results f Step 4 shw fr a given sample that datp (ng/ > tatp (ng/, bypass these calculatins and reprt catp = 0 and BSI = 100%. Step 4 Intermediate Calculatins Fllwing cmpletin f QG21S analyses, values must be cnverted t ATP cncentratins using the fllwing calculatins. Fr easy calculatins, utilize LumiCapture TM sftware available n Fr manual calculatins, see belw. 1. Ttal ATP (tatp) measures all ATP within a sample, including ATP frm living cells in additin t ATP that has been released frm dead cells. 1. Cellular ATP (catp) represents the amunt f ATP cntained within living cells and is a direct indicatin f ttal living bimass (quantity). catp ( pg ATP/ = tatp ( pg ATP/ datp ( pg ATP/ T cmmunicate results n the same basis as traditinal culture tests, catp results are cnverted int Micrbial Equivalents (ME s). This is based n the established cnversin that 1 E. cli-sized bacteria cntains pg (1 fg) f ATP. Aqua-tls 2010 Page 6 f 7

7 Test Kit Instructins QuenchGne21 Specialty (QG21S) catp ( ME = catp ( pg ATP / / 1ME pg ATP NOTE: Fr mre discussin n the quantity f ATP per cell, visit Because many f the traditinal culture-based methds reprt results in a similar fashin, it is smetimes cnvenient t reprt catp results in ME/mL using Scientific Ntatin (i.e. #.# x 10 # ) r n a Lg 10 frmat fr cmparisn purpses. 2. Bimass Stress Index (BSI) prvides a measure f the stress level (quality) f the micrbilgical cmmunity. datp ( pg ATP/ BSI (%) = tatp ( pg ATP/ Interpretatin Guidelines 100% Once QG21S catp and BSI results are calculated, micrbial cntrl can be evaluated. ATP-based measurements are extremely sensitive t changes in ttal micrbial quantity. In general, prcesses will have the best micrbial cntrl when catp is minimized and BSI is maximized. Aqua-tls ATP test kits can be used t audit micrbial quantity t reveal differences at different prcess lcatins in an effrt t quickly assess the ht spts within a prcess that require mre immediate attentin. Fr prcess cntrl, daily mnitring using ATP test kits will give yu true ttal micrbial quantity parameters t trend ver time against prcess characteristics and perfrmance. When utilizing ATP test kits it is imprtant t remember that every prcess is different. During audits, relative cmparisns frm pint t pint are a reliable means t assess yur prcess, while fr daily mnitring it is imprtant t establish a baseline trend befre making cntrl decisins. T get started, Aqua-tls prvides the fllwing catp guidelines in units f pg catp per ml: Fr BSI (when applicable), it can generally be interpreted that gd cntrl is achieved at levels f 75% r abve. Preventive actin shuld be taken at levels between 50% and 75%, and crrective actin shuld be taken at levels belw 50%. NOTE: These interpretatin guidelines are designed fr generic risk management guidance nly. Users are encuraged t establish their wn cntrl ranges n which t base prcess decisins. Ordering Infrmatin New t 2 nd generatin ATP technlgy? Start by rdering the Starter Kit, the Luminmeter and the test kit(s) f yur chice. T btain pricing infrmatin, inquire abut ther prducts and services, r t place an rder, cntact Aqua-tls fr Eurpe and MEA (please cntact LuminUltra fr the rest f the wrld). Aqua-tls Rute de Renault Flins-sur-Seine France Phne: Fax: cntact@aqua-tls.cm Orders generally ship within 2 business days fr Eurpe, 4 days fr ther cuntries, except Field Case upn special request. Yu will receive rder cnfirmatin via Fax r . exclusive distributr QG21S catp Interpretatin Guidelines Applicatin Prduct Quality Cntrl (Paints, Catings, Slurries) Gd Cntrl (pg catp/ < 100 Preventive Actin (pg catp/ 100 t 1,000 Crrective Actin (pg catp/ > 1,000 Lumitester is a trademark f Kikkman Crpratin; all ther trademarks are the prperty f LuminUltra Technlgies Ltd (Canada). Kits are manufactured by LuminUltra. Revised: 26/11/ Aqua-tls 2010 Page 7 f 7