Study of Non-Covalent Complexes by ESI-MS. By Quinn Tays
|
|
- Anthony Bradley
- 6 years ago
- Views:
Transcription
1 Study of Non-Covalent Complexes by ESI-MS By Quinn Tays
2 History Overview Background Electrospray Ionization How it is used in study of noncovalent interactions Uses of the Technique Types of molecules Drug Discovery Case Study Hydrophobicity of Proteins
3 History of ESI-MS The potential of ESI as a soft ionization technique was first reported by Malcolm Dole in 1968 who combined it with MS LC-MS with electrospray as the ionization source was first reported in 1984 Created by Masamichi Yamashita and John Fenn Fenn won the Nobel Prize (2002) for his Analysis of Biological Macromolecules
4 Background Electrospray Ionization Spray liquid through a capillary tube with an electric field Droplets form and as the solvent evaporates, and the ions will evaporate from the surface of the drops A skimmer only allows ions through ESI is a soft ionization technique Creates primarily in [M+H] + and [M+nH] n+ ions Result: Molecules with non covalent bonds may be ionized yet remain intact Kicman, A.; Parkin, M.; Iles, R. Molecular and Cellular Endocrinology ,
5 Background - ESI with Mass Spectrometry Mass spectra are commonly a small range containing [M+nH] n+ ions Analyzers over 4000 m/z are less common Most common is a quadropole mass analyzer Results have been successfully been obtained with most other mass analyzers Detector is commonly a high energy dynode
6 ESI-MS Pros and Cons Pros Can be combined with other methods of analysis (i.e. MS/MS) Scanning for Inborn Errors of Metabolism is used in clinical labs Ionization is soft enough that even covalent complexes will not fragment Cons Doesn t work for non-polar compounds Spectra of mixtures become very complex
7 Clinical Applications: Scanning for Inborn Errors of Metabolism (IEM) Rashed, M.; Bucknall, M.; Little, D. Clin Chem , ( IEM s are genetic disorders which usually cause a lack of certain protein used in breakdown of food Newborns are often affected by these diseases and must be scanned Some Examples are; Fructose Intolerance, Galactosemia, Maple Sugar Urine Disease (MSUD), and Phenylketonuria Missing some enzyme in the biochemical pathway Blood samples are tested with ESI-MS/MS and abnormal profiles are flagged using a computer algorithm Testing of the algorithm showed it was very good at flagging samples with the disease
8 Non-Covalent Complexes Primarily proteins are studied as ESI-MS is particularly useful for ionizing large molecules RNA and DNA are also studied Proteins Hydrogen bonding Ionic bonding Hydrophobic effect Nucleotide Complexes Hydrogen bonding between nucleosides
9 Non-Covalent Complexes Activity relationship studies between two molecules Concentration of bound complex vs. non bound complex reveals affinity for one another Competitive Binding Studies Substrate that binds with the highest affinity will appear with the largest signal in an ESI-MS Spectrum
10 Uses - Peptide-Peptide Affinities of binding of a protein to a peptide Ex. Ribonuclease cleaves phosphodiester bonds It is composed of a short polypeptide and a protein
11 Uses - Peptide-Metals Many proteins are bound to metals Important for catalytic function of enzymes Useful for determining Peptide Metal ion stoichiometry Ex. One atom of iron per heme (four heme groups for one hemoglobin)
12 Uses - Protein Subunits ESI-MS is useful for determining quaternary structure of proteins Homocomplexes or heterocomplexes 33% of proteins are multimeric, 80% of these are dimers or tetramers
13 Uses Protein-Nucleic Acid Complexes Proteins commonly serve as regulators of DNA and RNA It useful to know how/where they bind Knowing where on a sequence a protein can bind is useful for targeting tumor cells Competitive binding studies for specific nucleotide sequences
14 ESI-MS in Drug Discovery Binding affinities of compounds with a target Structure activity relationships of a known drug Vancomycin (blocks cell wall formation of bacteria) was screened for binding to cell wall analogues Screening of libraries of compounds for lead identification Compounds that interact strongly with the protein of interest will remain together for ionization This can be automated can screen large libraries easily
15 Case Study Probing the Hydrophobic Effect of Noncovalent Complexes by MS (Bich, C.; Baer, S.; Jecklin, M. J Am Soc Mass Spectrom , ) Occurs with chains of hydrophobic amino acids The hydrophobic effect occurs when proteins are in solution minimize the number of side chains exposed to water It drives folding of the protein and stabilizes the structure Procedure 4 proteins were purchased A quadropole TOF MS with ESI equipped was used to obtain MS Spectra MALDI TOF MS was also used to measure the proteins Results MALDI was found to effectively give a picture of the non-covalent interactions of the proteins however ESI-MS was not found to be effective at studying compounds exhibiting the hydrophobic effect ESI-MS detected compounds with strong hydrogen bonds and compounds with mixed polar interactions and hydrophobic interactions Compounds with hydrogen bonding survived the transfer to gas
16 Conclusion ESI-MS works well for non-covalent complexes due to its lack of fragmenation ESI-MS is widely used in the study of proteins A wide variety of biological molecules can be analyzed An essential tool for early steps of drug discovery
17 References [1] Kicman, A.; Parkin, M.; Iles, R. Molecular and Cellular Endocrinology , [2] Rashed, M.; Bucknall, M.; Little, D. Clin Chem , [3] Bich, C.; Baer, S.; Jecklin, M. J Am Soc Mass Spectrom , [4] Ho, C.; Lam, C.; Chan, M. Clin Biochem Rev , [5] Loo, J. Mass Spectrometry Reviews , 1 23.
Chapter 2 The Chemistry of Biology. Dr. Ramos BIO 370
Chapter 2 The Chemistry of Biology Dr. Ramos BIO 370 2 Atoms, Bonds, and Molecules Matter - all materials that occupy space and have mass Matter is composed of atoms. Atom simplest form of matter not divisible
More informationthe spatial arrangement of atoms in a molecule and the chemical bonds that hold the atoms together Chemical structure Covalent bond Ionic bond
Chemical structure the spatial arrangement of atoms in a molecule and the chemical bonds that hold the atoms together Covalent bond bond formed by the sharing of valence electrons between atoms Ionic bond
More informationTeacher Instructions
Teacher Instructions To print handouts for students Go to File print, change Print what: to handouts, change # per page if desired to enlarge slides on page Change Print range to slides and type in slide
More informationFundamentals of Mass Spectrometry. Fundamentals of Mass Spectrometry. Learning Objective. Proteomics
Mass spectrometry (MS) is the technique for protein identification and analysis by production of charged molecular species in vacuum, and their separation by magnetic and electric fields based on mass
More informationHole s Human Anatomy and Physiology Eleventh Edition. Chapter 2
Hole s Human Anatomy and Physiology Eleventh Edition Shier Butler Lewis Chapter 2 1 Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. CHAPTER 2 CHEMICAL BASIS OF
More informationTANDEM MASS SPECTROSCOPY
TANDEM MASS SPECTROSCOPY 1 MASS SPECTROMETER TYPES OF MASS SPECTROMETER PRINCIPLE TANDEM MASS SPECTROMETER INSTRUMENTATION QUADRAPOLE MASS ANALYZER TRIPLE QUADRAPOLE MASS ANALYZER TIME OF FLIGHT MASS ANALYSER
More informationEnergy Transformation and Metabolism (Outline)
Energy Transformation and Metabolism (Outline) - Definitions & Laws of Thermodynamics - Overview of energy flow ecosystem - Biochemical processes: Anabolic/endergonic & Catabolic/exergonic - Chemical reactions
More informationENV SCI 22 GROUP QUIZ WEEK 2
ENV SCI 22 GROUP QUIZ WEEK 2 ph OF ACIDS AND BASES 1) A decrease of one unit in the ph scale above represents a tenfold increase in the hydrogen ion concentration of a solution. For example, a solution
More informationThe Chemistry of Life
The Chemistry of Life Things you should be able to do 1. Describe how the unique properties of water support life on Earth. 2. Explain how carbon is uniquely suited to form biological macromolecules. 3.
More informationFoundations in Microbiology Seventh Edition
Lecture PowerPoint to accompany Foundations in Microbiology Seventh Edition Talaro Chapter 2 The Chemistry of Biology Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
More informationMatter and Substances Section 3-1
Matter and Substances Section 3-1 Key Idea: All matter is made up of atoms. An atom has a positively charges core surrounded by a negatively charged region. An atom is the smallest unit of matter that
More informationREVIEW 1: BIOCHEMISTRY UNIT. A. Top 10 If you learned anything from this unit, you should have learned:
Period Date REVIEW 1: BIOCHEMISTRY UNIT A. Top 10 If you learned anything from this unit, you should have learned: 1. All living matter made up of CHONPS 2. Bonds a. covalent bonds are strong b. hydrogen
More informationCopy into Note Packet and Return to Teacher
Copy into Note Packet and Return to Teacher Section 1: Nature of Matter Objectives: Differentiate between atoms and elements. Analyze how compounds are formed. Distinguish between covalent bonds, hydrogen
More informationElements and Isotopes
Section 2-1 Notes Atoms Life depends on chemistry. The basic unit of matter is the atom. Atoms are incredibly small The subatomic particles that make up atoms are protons, neutrons, and electrons. Parts
More informationUNIT 1: BIOCHEMISTRY
UNIT 1: BIOCHEMISTRY UNIT 1: Biochemistry Chapter 6.1: Chemistry of Life I. Atoms, Ions, and Molecules A. Living things consist of atoms of different elements 1. An atom is the smallest basic unit of matter
More informationChemistry of Life. Chapter Two
Chemistry of Life Chapter Two 1 Biology and Chemistry Biology = study of life Chemistry = study of matter and the changes it undergoes Matter anything that takes up space and has mass Life is made up of
More informationLecture 15: Introduction to mass spectrometry-i
Lecture 15: Introduction to mass spectrometry-i Mass spectrometry (MS) is an analytical technique that measures the mass/charge ratio of charged particles in vacuum. Mass spectrometry can determine masse/charge
More informationCh 3: Chemistry of Life. Chemistry Water Macromolecules Enzymes
Ch 3: Chemistry of Life Chemistry Water Macromolecules Enzymes Chemistry Atom = smallest unit of matter that cannot be broken down by chemical means Element = substances that have similar properties and
More informationMolecular Mass Spectrometry
Molecular Mass Spectrometry Mass Spectrometry: capable of providing information about (1) Elemental composition of samples of matter: atomic mass (2) Structures of inorganic, organic, and biological molecules
More informationEnzyme Catalysis & Biotechnology
L28-1 Enzyme Catalysis & Biotechnology Bovine Pancreatic RNase A Biochemistry, Life, and all that L28-2 A brief word about biochemistry traditionally, chemical engineers used organic and inorganic chemistry
More informationChapter 2 The Chemistry of Life
Chapter 2 The Chemistry of Life I. Water Liquid Naturally occurring It expands liquid to solid Covers more than 75% of our surface Most abundant in living organisms most important inorganic compound for
More informationDescribe how proteins and nucleic acids (DNA and RNA) are related to each other.
Name Date Molecular Biology Review Part 1 IB Papers Topic 2.1 Molecules to Metabolism Living organisms control their composition by a complex web of chemical interactions. Be able to: Explain how molecular
More informationThe biomolecules of terrestrial life
Functional groups in biomolecules Groups of atoms that are responsible for the chemical properties of biomolecules The biomolecules of terrestrial life Planets and Astrobiology (2017-2018) G. Vladilo 1
More informationBiological Macromolecules
Introduction for Chem 493 Chemistry of Biological Macromolecules Dr. L. Luyt January 2008 Dr. L. Luyt Chem 493-2008 1 Biological macromolecules are the molecules of life allow for organization serve a
More informationBiological Mass Spectrometry
Biochemistry 412 Biological Mass Spectrometry February 13 th, 2007 Proteomics The study of the complete complement of proteins found in an organism Degrees of Freedom for Protein Variability Covalent Modifications
More informationBIOCHEMISTRY 10/9/17 CHEMISTRY OF LIFE. Elements: simplest form of a substance - cannot be broken down any further without changing what it is
BIOCHEMISTRY CHEMISTRY OF LIFE Elements: simplest form of a substance - cannot be broken down any further without changing what it is THE ATOM Just like cells are the basic unit of life, the ATOM is the
More informationBIOCHEMISTRY NOTES - UNIT 2-
BIOCHEMISTRY NOTES - UNIT 2- ATOMS - the basic unit of matter. Contains subatomic particles o (+ charge) o (no charge/neutral) o (- charge) Protons and neutrons have about the same mass. Electrons are
More informationChapter 002 The Chemistry of Biology
Chapter 002 The Chemistry of Biology Multiple Choice Questions 1. Anything that occupies space and has mass is called A. Atomic B. Living C. Matter D. Energy E. Space 2. The electrons of an atom are A.
More information2.1 Atoms, Ions, and Molecules
2.1 Atoms, Ions, and Molecules Living things consist of atoms of different elements. An atom is the smallest basic unit of matter. An element is one type of atom. 6 elements make up 99% of all living things
More informationMass Spectrometry. Electron Ionization and Chemical Ionization
Mass Spectrometry Electron Ionization and Chemical Ionization Mass Spectrometer All Instruments Have: 1. Sample Inlet 2. Ion Source 3. Mass Analyzer 4. Detector 5. Data System http://www.asms.org Ionization
More informationLiving and nonliving matter is composed of atoms.
Chemistry Topics Covered Atomic structure and interactions Properties of Water Biological Molecules: carbohydrates, lipids, nucleic acids, and proteins Central Dogma: DNA à RNA à Protein Protein Structure:
More informationUnit 1: Chemistry - Guided Notes
Scientific Method Notes: Unit 1: Chemistry - Guided Notes 1 Common Elements in Biology: Atoms are made up of: 1. 2. 3. In order to be stable, an atom of an element needs a full valence shell of electrons.
More informationChapter 2 Chemistry of Life
Chapter 2 Chemistry of Life Section 2.1 Atoms, Ions and Molecules Section 2.2 Properties of water Section 2.3 Carbon-based Molecules Section 2.4 Chemical Reactions Section 2.5 - Enzymes 1 Atoms, Ions and
More informationChemistry in Biology. Section 1. Atoms, Elements, and Compounds
Section 1 Atoms, Elements, and Compounds Atoms! Chemistry is the study of matter.! Atoms are the building blocks of matter.! Neutrons and protons are located at the center of the atom.! Protons are positively
More informationAtoms. Atoms 9/9/2015
The Chemistry of Life The Nature of Matter, Water,Carbon Compounds, Chemical Reactions and Enzymes The Nature of Matter B.1.9 Both living and nonliving things are composed of compounds, which are themselves
More informationChapter 2: Chemical Basis of Life I. Introduction A. The study of chemistry is essential for the study of physiology because
Shier, Butler, and Lewis: Hole s Human Anatomy and Physiology, 11 th ed. Chapter 2: Chemical Basis of Life Chapter 2: Chemical Basis of Life I. Introduction A. The study of chemistry is essential for the
More informationBIOCHEMISTRY/MOLECULAR BIOLOGY
Enzymes Activation Energy Chemical reactions require an initial input of energy activation energy large biomolecules are stable must absorb energy to break bonds cellulose energy CO 2 + H 2 O + heat Activation
More informationThe Chemistry of Life.
The Chemistry of Life http://www.chem.ufl.edu/~itl/2045_s00/matter/fg01_011.gif Atom: the smallest unit of matter Subatomic particles 1. neutron a. In nucleus b. No charge c. Weight 1dalton 2. proton a.
More informationPTYS 214 Spring Announcements. Midterm #1 on Tuesday! Be on time! No one enters after the first person leaves! Do your homework!
PTYS 214 Spring 2018 Announcements Midterm #1 on Tuesday! Be on time! No one enters after the first person leaves! Do your homework! 1 Last time - Properties of Life Organization, energy utilization, homeostasis,
More informationChemistry of Life 10/1/2010. What makes up the chemistry of life?
A. Students will be able to identify and define the parts of an atom. Chemistry of Life At the Completion of this Unit, Students will be able to: A. Identify and define the parts of an atom. B. Demonstrate
More informationLecture 8: Mass Spectrometry
intensity Lecture 8: Mass Spectrometry Relative abundance m/z 1 Ethylbenzene CH 2 CH 3 + m/z = 106 CH 2 + m/z = 91 C 8 H 10 MW = 106 CH + m/z = 77 + 2 2 What information can be obtained from a MS spectrum?
More informationBiology 30 The Chemistry of Living Things
Biology 30 The Chemistry of Living Things Hierarchy of organization: Chemistry: MATTER: Periodic Table: ELEMENT: Ex. oxygen, gold, copper, carbon COMPOUND: Ex. salt (NaCl), H 2 O ELEMENTS ESSENTIAL TO
More informationAn Introduction to Metabolism. Chapter 8
An Introduction to Metabolism Chapter 8 METABOLISM I. Introduction All of an organism s chemical reactions Thousands of reactions in a cell Example: digest starch use sugar for energy and to build new
More information2/18/2013 CHEMISTRY OF CELLS. Carbon Structural Formations. 4 Classes of Organic Compounds (biomolecules)
CHEMISTRY OF CELLS 11 elements make up all organisms C, O, N, H: 96% weight of human body ORGANIC CHEMISTRY Organic compounds: contain C Inorganic compounds: no C Bonding and Structural Formulas H and
More informationChapter 1. Topic: Overview of basic principles
Chapter 1 Topic: Overview of basic principles Four major themes of biochemistry I. What are living organism made from? II. How do organism acquire and use energy? III. How does an organism maintain its
More informationBasic Chemistry. Chapter 2 BIOL1000 Dr. Mohamad H. Termos
Basic Chemistry Chapter 2 BIOL1000 Dr. Mohamad H. Termos Chapter 2 Objectives Following this chapter, you should be able to describe: - Atoms, molecules, and ions - Composition and properties - Types of
More informationBio110 Lab 3: Basic Chemistry A. Carranza
NAME Basic Chemistry The following chart lists the important elements found in cytoplasm by weight. On the chart, fill in the symbol and the number of electrons found in each element Use the periodic table
More informationUnit Two Chemistry of the Human Body
I. Introduction to atoms Unit Two Chemistry of the Human Body A. Chemistry is the branch of science that concerns itself with the structure of matter, including the interaction between atoms. 1. Atoms-
More informationChapter Two Test Chemistry. 1. If an atom contains 11 protons and 12 neutrons, its atomic number is A. 1 C. 12 B. 11 D. 23
Name Chapter Two Test Chemistry 1. If an atom contains 11 protons and 12 neutrons, its atomic number is A. 1 C. 12 B. 11 D. 23 2. The nucleus is made up of all of the following: A. Electrons C. Protons
More informationChapter 6 Chemistry in Biology. 6.1 Atoms, Elements & Compounds 6.2 Chemical Reactions 6.3 Water and Solutions 6.4 The Building Blocks of Life
Chapter 6 Chemistry in Biology 6.1 Atoms, Elements & Compounds 6.2 Chemical Reactions 6.3 Water and Solutions 6.4 The Building Blocks of Life 6.1 Atoms, Elements, and Compounds Main idea: Matter is composed
More informationStudy Guide: Basic Chemistry, Water, Life Compounds and Enzymes
Study Guide: Basic Chemistry, Water, Life Compounds and Enzymes 1. Lipids are good energy-storage molecules because a) the can absorb a large amount of energy while maintaining a constant temperature b)
More informationThe Chemistry of Microbiology
PowerPoint Lecture Presentations prepared by Mindy Miller-Kittrell, North Carolina State University C H A P T E R 2 The Chemistry of Microbiology Atoms Matter anything that takes up space and has mass
More informationBiology. Chapter 2 Notes
Biology Chapter 2 Notes Section 1: Nature of Matter Objectives: 1) Differentiate between atoms and elements 2) Analyze how compounds are formed 3) Distinguish between covalent bonds, hydrogen bonds and
More informationLC-MS/MS in the Clinical Laboratory. Jo Adaway
LC-MS/MS in the Clinical Laboratory Jo Adaway LC-MS/MS in the clinical laboratory LC-MS/MS is mainly used for quantification of analytes used in the diagnosis and monitoring of disease Semi-quantitative
More informationU2.1.1: Molecular biology explains living processes in terms of the chemical substances involved (Oxford Biology Course Companion page 62).
Unit 11: Biochemistry Study Guide U2.1.1: Molecular biology explains living processes in terms of the chemical substances involved (Oxford Biology Course Companion page 62). 1. Define molecular biology.
More information2/25/2013. Electronic Configurations
1 2 3 4 5 Chapter 2 Chemical Principles The Structure of Atoms Chemistry is the study of interactions between atoms and molecules The atom is the smallest unit of matter that enters into chemical reactions
More informationChapter 02 Chemical Composition of the Body
Chapter 02 Chemical Composition of the Body 1. In an atom, the number of Student: A. Protons always equals the number of neutrons B. Of protons always equals the number of electrons C. Of neutrons always
More informationChapter 2: Fundamentals of Chemistry. Question Type: Multiple Choice. 1) Which of the following pairs is mismatched?
Microbiology Principles and Explorations 9th Edition Black TEST BANK Full clear download at: https://testbankreal.com/download/microbiology-principles-explorations- 9th-edition-black-test-bank/ Microbiology
More informationChapter 02 Chemistry of Life
Chapter 02 Chemistry of Life Multiple Choice Questions 1. The smallest unit of matter is the A. molecule. B. atom. C. compound. D. isotope. HAPS Objective: C.01.03 Compare and contrast the terms atoms,
More informationBiology Chapter 2: The Chemistry of Life. title 4 pictures, with color (black and white don t count!)
33 Biology Chapter 2: The Chemistry of Life title 4 pictures, with color (black and white don t count!) 34 Chapter 2: The Chemistry of Life Goals Highlight all unknown words 35-36 Chapter 2: The Chemistry
More informationEVPP 110 Lecture Exam #1 Study Questions Fall 2003 Dr. Largen
EVPP 110 Lecture Exam #1 Study Questions Fall 2003 Dr. Largen These study questions are meant to focus your study of the material for the first exam. The absence here of a topic or point covered in lecture
More informationChapter 2 Chemical Aspects of Life
Chapter 2 Chemical Aspects of Life Multiple Choice Questions 1. Anything that has weight and occupies space can be described as A. an atom. B. matter. C. a compound. D. a molecule. #1 Learning Outcome:
More informationWhat to do about the world s most deadly compound DIHYDROGEN MONOXIDE (DHMO)
What to do about the world s most deadly compound DIHYDROGEN MONOXIDE (DHMO) Unit 2 Bio-molecules and Biochemistry The Chemistry of Life It all starts with Water Life depends on water! Why do you think
More informationIntroduction to Life Science. BSC 1005 Fall 2011 Homework 1! Connect Due Date: 9/18/ :59PM. Multiple Choice Portion
Introduction to Life Science BSC 1005 Fall 2011 Homework 1 Connect Due Date: 9/18/2011 11:59PM Instructions Complete this homework assignment as the material is covered in class. You may refer to any of
More informationBiology of Humans: Concepts, Applications, and Issues, 6e (Goodenough) Chapter 2 Chemistry Comes to Life
Biology of Humans: Concepts, Applications, and Issues, 6e (Goodenough) Chapter 2 Chemistry Comes to Life 2.1 Multiple Choice Questions 1) A neutral atom must contain. A) an equal number of protons and
More informationMULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.
MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) A neutral atom must contain. A) an equal number of protons, neutrons, and electrons B) an equal
More informationChapter 6 Chemistry in Biology
Section 1: Atoms, Elements, and Compounds Section 2: Chemical Reactions Section 3: Water and Solutions Section 4: The Building Blocks of Life Click on a lesson name to select. 6.1 Atoms, Elements, and
More informationChemical Aspects of Mass Spectrometry
Chemical Aspects of Mass Spectrometry Abraham Badu Chemistry and Biochemistry, OSU July 12, 217 Spread of MS by Discipline https://masspec.scripps.edu/ mass spectrometry 2 1 Current Challenges in Mass
More informationLecture 8: Mass Spectrometry
intensity Lecture 8: Mass Spectrometry Relative abundance m/z 1 Ethylbenzene experiment CH 2 CH 3 + m/z = 106 CH 2 + m/z = 91 C 8 H 10 MW = 106 CH + m/z = 77 + 2 2 What information can we get from MS spectrum?
More informationChemistry Review. Structure of an Atom. The six most abundant elements of life. Types of chemical bonds. U n i t 2 - B i o c h e m i s t r y
Chemistry Review Structure of an Atom are organized into shells or levels around the nucleus. Atoms are most stable when their outer or valence shell is. The six most abundant elements of life Types of
More informationChem Lecture 3 Hemoglobin & Myoglobin
Chem 452 - Lecture 3 Hemoglobin & Myoglobin 111003 Hemoglobin (Hb) and Myoglobin (Mb) function as oxygen transport and storage molecules in higher organisms. There functions have been long studied and,
More information1.Matter and Organic Compounds Matter =
The Chemistry of Life Notes Unit 2 1.Matter and Organic Compounds Matter = All things are made of matter Name Matter is made up of substances Chemical substance = definite composition throughout Either
More informationChemistry of Life Essential Questions
Chemistry of Life Essential Questions VMHS Standards 8.6b; 8.6c; 1h; 4e; 4f; 5a;1b; 1. What is an atom? What are elements? An atom is the basic unit of o Consist of,, and An element is a type of atom o
More informationMolecular Mass Spectrometry
Molecular Mass Spectrometry Mass Spectrometry: capable of providing information about (1) Elemental composition of samples of matter: atomic mass (2) Structures of inorganic, organic, and biological molecules
More information2: CHEMICAL COMPOSITION OF THE BODY
1 2: CHEMICAL COMPOSITION OF THE BODY CHAPTER OVERVIEW This chapter provides an overview of basic chemical principles that are important to understanding human physiological function and ultimately homeostasis.
More informationSaba Al Fayoumi. Tamer Barakat. Dr. Mamoun Ahram + Dr. Diala Abu-Hassan
1 Saba Al Fayoumi Tamer Barakat Dr. Mamoun Ahram + Dr. Diala Abu-Hassan What is BIOCHEMISTRY??? Biochemistry = understanding life Chemical reactions are what makes an organism (An organism is simply atoms
More informationATP ATP. The energy needs of life. Living economy. Where do we get the energy from? 9/11/2015. Making energy! Organisms are endergonic systems
Making energy! ATP The energy needs of life rganisms are endergonic systems What do we need energy for? synthesis building biomolecules reproduction movement active transport temperature regulation 2007-2008
More informationCh. 3 Metabolism and Enzymes
Ch. 3 Metabolism and Enzymes Originally prepared by Kim B. Foglia. Revised and adapted by Nhan A. Pham Flow of energy through life Life is built on chemical reactions that enable energy to flow through
More information2.1 Atoms, Ions, and Molecules. 2.1 Atoms, Ions, and Molecules. 2.1 Atoms, Ions, and Molecules. 2.1 Atoms, Ions, and Molecules
All living things are based on atoms and their interactions. Living things consist of atoms of different elements. An atom is the smallest basic unit of matter. An element is one type of atom. ydrogen
More informationAnalysis of Polar Metabolites using Mass Spectrometry
Analysis of Polar Metabolites using Mass Spectrometry TransMed Course: Basics in Clinical Proteomics and Metabolomics. Oct 10-19, 2012 dd.mm.yyyy Vidya Velagapudi, Ph.D, Adjunct Professor Head of the Metabolomics
More informationMULTIPLE CHOICE. Circle the one alternative that best completes the statement or answers the question.
Summer Work Quiz - Molecules and Chemistry Name MULTIPLE CHOICE. Circle the one alternative that best completes the statement or answers the question. 1) The four most common elements in living organisms
More informationPress Release: The Nobel Prize in Chemistry October 2002 The Royal Swedish Academy of Sciences has decided to award the Nobel Prize in
Press Release: The Nobel Prize in Chemistry 2002 9 October 2002 The Royal Swedish Academy of Sciences has decided to award the Nobel Prize in Chemistry for 2002 for the development of methods for identification
More informationNature of matter. Chemical bond is a force that joins atoms
Nature of matter Atom the smallest unit of matter that cannot be broken down by chemical means The subatomic particles of an atom consist of protons, neutrons and electrons Element is a pure substance
More information2.1 The Nature of Matter
2.1 The Nature of Matter Lesson Objectives Identify the three subatomic particles found in atoms. Explain how all of the isotopes of an element are similar and how they are different. Explain how compounds
More informationOther Methods for Generating Ions 1. MALDI matrix assisted laser desorption ionization MS 2. Spray ionization techniques 3. Fast atom bombardment 4.
Other Methods for Generating Ions 1. MALDI matrix assisted laser desorption ionization MS 2. Spray ionization techniques 3. Fast atom bombardment 4. Field Desorption 5. MS MS techniques Matrix assisted
More informationCharged amino acids (side-chains)
Proteins are composed of monomers called amino acids There are 20 different amino acids Amine Group Central ydrocarbon N C C R Group Carboxyl Group ALL amino acids have the exact same structure except
More informationReview_Unit 2 Biochemistry
Review_Unit 2 Biochemistry Basic Chemistry 1. What is an element? A substance that cannot be broken down into smaller particles. 2. What are atoms? The smallest part of an element that still maintains
More information2.1. KEY CONCEPT All living things are based on atoms and their interactions. 34 Reinforcement Unit 1 Resource Book
2.1 ATOMS, IONS, AND MOLECULES KEY CONCEPT All living things are based on atoms and their interactions. All matter, whether living or nonliving, is made of the same tiny building blocks, called atoms.
More informationBloom's Level: 1. Remember Learning Outcome: Describe the structure of atoms. Section: Topic: Chemistry
Chapter 02 The Chemistry of Life Multiple Choice Questions 1. The primary elements making up living organisms are A. carbon, hydrogen, oxygen, and calcium. B. carbon, oxygen, iron, and chlorine. C. carbon,
More informationReading Assignments. A. Genes and the Synthesis of Polypeptides. Lecture Series 7 From DNA to Protein: Genotype to Phenotype
Lecture Series 7 From DNA to Protein: Genotype to Phenotype Reading Assignments Read Chapter 7 From DNA to Protein A. Genes and the Synthesis of Polypeptides Genes are made up of DNA and are expressed
More informationBIOCHEMISTRY GUIDED NOTES - AP BIOLOGY-
BIOCHEMISTRY GUIDED NOTES - AP BIOLOGY- ELEMENTS AND COMPOUNDS - anything that has mass and takes up space. - cannot be broken down to other substances. - substance containing two or more different elements
More informationFigure ) Letter E represents a nucleic acid building block known as a. Answer: nucleotide Diff: 3 Page Ref: 54
Essentials of Human Anatomy and Physiology, 10e (Marieb) Chapter 2 Basic Chemistry 2.1 Short Answer Figure 2.1 Using Figure 2.1, identify the following: 1) Which letter represents a carbohydrate polymer?
More informationProtein Structure. W. M. Grogan, Ph.D. OBJECTIVES
Protein Structure W. M. Grogan, Ph.D. OBJECTIVES 1. Describe the structure and characteristic properties of typical proteins. 2. List and describe the four levels of structure found in proteins. 3. Relate
More informationDo Now. What is a catalyst? PASS UP LABS!
Do Now What is a catalyst? PASS UP LABS! Do Now What is a compound? Give an example Name 3 elements found in our body. What are the 3 parts of an atom and where are they located? Do Now What are carbohydrates
More informationChapter 02 Chemistry of Life
Maders Understanding Human Anatomy and Physiology 9th Edition Longenbaker Test Bank Full Download: http://testbanklive.com/download/maders-understanding-human-anatomy-and-physiology-9th-edition-longenbaker
More informationChapter 02 The Chemistry of Biology
Chapter 02 The Chemistry of Biology Multiple Choice Questions 1. Anything that occupies space and has mass is called A. atomic. B. living. C. matter. D. energy. E. space. Learning Outcome: 02.01 Explain
More informationStructure of the α-helix
Structure of the α-helix Structure of the β Sheet Protein Dynamics Basics of Quenching HDX Hydrogen exchange of amide protons is catalyzed by H 2 O, OH -, and H 3 O +, but it s most dominated by base
More informationUnit 2: The Properties of Water, Organic Macromolecules, Enzymes, Digestion (questions)
Table 1: ph Values of Common Substances 1. Observe Table 1, which substance has the highest concentration of H+ ions? a. Water b. Baking soda solution c. Lemon juice d. Sodium hydroxide solution 2. Which
More informationBST 226 Statistical Methods for Bioinformatics David M. Rocke. January 22, 2014 BST 226 Statistical Methods for Bioinformatics 1
BST 226 Statistical Methods for Bioinformatics David M. Rocke January 22, 2014 BST 226 Statistical Methods for Bioinformatics 1 Mass Spectrometry Mass spectrometry (mass spec, MS) comprises a set of instrumental
More informationLiving and nonliving things are all made of elements. It is the way that atoms combine that give every element a different characteristic.
Living and nonliving things are all made of elements. It is the way that atoms combine that give every element a different characteristic. 98% of the body is made of only 6 elements The 6 elements are:
More information