Foundations in Microbiology Seventh Edition

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1 Lecture PowerPoint to accompany Foundations in Microbiology Seventh Edition Talaro Chapter 2 The Chemistry of Biology Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display.

2 2.1 Atoms, Bonds, and Molecules Matter - all materials that occupy space and have mass Matter is composed of atoms Atom - simplest form of matter not divisible into simpler substances Protons: (+) subatomic particles Neutrons: neutral subatomic particles Electrons: (-) subatomic particles 2

3 Figure 2.1a Three-dimensional model 3

4 Figure 2.1b Simple model 4

5 Different Types of Atoms All atoms share the same fundamental structure Element - pure substances with a characteristic number of protons, neutrons, and electrons and predictable chemical behaviors 5

6 Table 2.1 The Major Elements of Life 6

7 Characteristics of Elements Atomic number number of protons Mass number number of protons and neutrons Isotopes variant forms of the same element that differ in the number of neutrons Atomic weight average mass numbers of all isotopic forms Electron orbitals volumes of space surrounding the atomic nucleus where electrons are likely to be found 7

8 Figure 2.1 Models of atomic structure 8

9 Figure 2.2 Biologically important atoms 9

10 Bonds and Molecules Molecule - a chemical substance that results from the combination of two or more atoms Compounds - molecules that are combinations of two or more different elements Formula/Mass weight - sum of all of the atomic masses of the atoms a molecule contains Chemical bonds - when 2 or more atoms share, donate, or accept electrons to form molecules and compounds 3 types: covalent, ionic, and hydrogen 10

11 3 Types of Chemical Bonds 1. Covalent bonds electrons are shared among atoms Polar covalent bonds unequal sharing Nonpolar covalent bonds equal sharing 2. Ionic bonds electrons are transferred to one atom forming positively charged cations and negatively charged anions 3. Hydrogen bonds weak bonds between hydrogen and other atoms 11

12 Figure 2.3 Three types of bonds 12

13 Figure 2.4 Covalent bonds 13

14 Figure 2.5 Polar molecule 14

15 Figure 2.6 Ionic bonding 15

16 Figure 2.7 Ionization 16

17 Figure 2.8 Hydrogen bonding 17

18 Electron Transfer and Oxidation- Reduction Reactions Energy exchange in cells is a result of the movement of electrons from one molecule to another. Oxidation the loss of electrons Reduction the gaining of electrons Redox reactions essential to biochemical processes 18

19 Figure 2.9 Oxidation-reduction reaction 19

20 Chemical Shorthand Reactants - molecules starting a reaction Products - substances left by a reaction Synthesis reaction - the reactants bond together in a manner that produces an entirely new molecule S + O 2 SO 2 20

21 Figure 2.10 Molecular and Structural Formulas 21

22 Solutions: Mixtures of Molecules Solution a mixture of one or more substances called solutes, dispersed in a dissolving medium called a solvent Solutes Na + & Cl - Solvent H 2 O 22

23 Most biological activities occur in aqueous (water-based) solutions Hydrophilic molecules - dissolve in water Hydrophobic molecules - repel water Amphipathic molecules - have both hydrophilic and hydrophobic properties 23

24 Figure 2.12 Hydration spheres formed around ions 24

25 Acidity, Alkalinity, and the ph Scale Ionization of H 2 O releases hydrogen ions [H + ] and hydroxyl ions [OH - ] ph scale ranges from 0 to 14, expresses the concentration of H + ions ph is the negative logarithm of the concentration of H + ions. ph 2 = 0.01 moles/l H + ions ph 14 = moles/l H + ions 25

26 Figure 2.13 The ph scale 26

27 Table

28 The Chemistry of Carbon and Organic Compounds Organic chemicals compounds containing carbon bonded to hydrogens 28

29 Carbon is the fundamental element of life Contains 4 atoms in its outer orbital Can form single, double, or triple covalent bonds Can form linear, branched, or ringed molecules 29

30 Figure 2.14 The versatility of bonding in carbon 30

31 Functional Groups of Organic Compounds Accessory molecules that bind to organic compounds Confer unique reactive properties on the whole molecule 31

32 2.2 Macromolecules Biochemicals are organic compounds produced by living things Macromolecules: large compounds assembled from smaller subunits Monomer: a repeating subunit Polymer: a chain of monomers 32

33 Macromolecules 1. Carbohydrates monosaccharides, disaccharides, polysaccharides 2. Lipids triglycerides (fats and oils), phospholipids, steroids 3. Proteins 4. Nucleic acids DNA, RNA 33

34 Carbohydrates Sugars and polysaccharides General formula (CH 2 O) n Aldehydes and ketones Saccharide: simple carbohydrate Monosaccharide: 3-7 carbons Disaccharide: two monosaccharides Polysaccharide: five or more monosaccharides 34

35 Figure 2.15a Common classes of carbohydrates 35

36 Figure 2.15b Common classes of carbohydrates 36

37 Carbohydrates Subunits linked by glycosidic bonds Dehydration synthesis: loss of water in a polymerization reaction 37

38 Carbohydrates Functions cell structure, adhesion, and metabolism 38

39 Lipids Long or complex, hydrophobic, C - H chains Triglycerides, phospholipids in membranes, steroids like cholesterol Functions Triglycerides energy storage Phospholipid major cell membrane component Steroids cell membrane component 39

40 Figure 2.18b Triglycerides 40

41 Figure 2.19 Phospholipids - membrane molecules 41

42 Figure 2.20 Membrane 42

43 Proteins Predominant molecules in cells Monomer amino acids 20 Polymer peptide, polypeptide, protein Subunits linked by peptide bonds Fold into very specific 3-D shapes Functions support, enzymes, transport, defense, movement 43

44 44

45 Figure 2.21 Formation of a peptide bond 45

46 46

47 Nucleic Acids DNA and RNA Monomer nucleotide DNA deoxyribonucleic acid A,T,C,G nitrogen bases Double helix Function hereditary material RNA ribonucleic acid A,U,C,G nitrogen bases Function organize protein synthesis 47

48 48

49 Figure 2.24 Sugars and nitrogen bases of DNA and RNA 49

50 DNA is formed by two very long polynucleotide strands linked along their length by hydrogen bonds Double Helix of DNA 50

51 Passing on the Genetic Message Each strand is copied Replication is guided by base pairing End result is two separate double strands 51

52 ATP: The Energy Molecule of Cells Adenosine triphosphate Nucleotide - adenine, ribose, three phosphates Function - transfer and storage of energy Insert figure 2.27 a ATP molecule 52

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