HSCCC, (H igh - Speed Countercurrent Chro2 matography, HSCCC) 2 ,, HSCCC, HPLC HSCCC

Transcription

1 2007 Vol. 9 No. 1 * * * (/ ) :(HSCCC) : (H igh - Speed Countercurrent Chro2 matography, HSCCC)2,, : : * ( KM ) ;,:; ( ),: * * :,,,Tel: , E2mail: Caoxl@ th. btbu. edu. cn 1995, 5 [ 1-5 ], [ 6, 7 ] HSCCC,,, HSCCC,, (HPLC), HPLC HSCCC, HSCCC, HSCCC 2030,,,,, 54 W orld Science and Technology /M odernization of Traditional Chinese M edicine and M ateria M edica

2 ( ),,,,,, - (UV - V IS), ( ELSD ),,,,,, HSCCC,,HPLC, HSCCC -,, HSCCC,, HSCCC [ 7 ] HSCCC,,,,pH,,,1 [ 5 ],,,,,,,,, 10, HSCCC ( EGCG) ( ECG) ( GCG) 2, 3, 5, W orld Science and Technology /M odernization of Traditional Chinese M edicine and M ateria M edica 55

3 2007 Vol. 9 No. 1 HSCCC [ 8 ] HSCCC HSCCC HSCCC, 1mm, 50mL(multilayer coil p lanet centrifuge,mlcpc) HSCCC HPLC,,,, [ 9 ] HSCCC HSCCC,,,, HSCCC [ 10, 11 ],,, (MS),HSCCC HSCCC,,, HSCCC, HSCCC,, HSCCC [ 12, 13 ],, ( ) 10,,,, [ 14 ] HSCCC,,,,,,,,,,, HSCCC,, HSCCC 1. HSCCC,HSCCC, 35mL, mm, HSCCC MS [ 15, 16 ],HSCCC MS,,, HSCCC 2. HSCCC HSCCC, HSCCCHPLC HSCCCHPLC,, HPLC 75% [ 17 ],, HSCCC 56 W orld Science and Technology /M odernization of Traditional Chinese M edicine and M ateria M edica

4 , HSCCC,,, B runel Sutherland [ 18 ],,5mL 5L,1000, ( < 10m in),,hsccc, B runel Dynam ic Extraction18L (CCC), 10LCCC 3. HSCCC HSCCC [ 19 ], ph - 10g [ 20 ], HSCCC HSCCC,,, HSCCC [ 21, 22 ] 4. HSCCC, HSCCC,,, ;, HSCCC,,,,,,, HSCCC, HSCCC,, - 30,, HSCCC,,,, HSCCC 1 Conway W D and Petroski R J ( eds. ) Modern Countercurrent Chroma2 tography. ACS Symposium Series Vol Foucault A. Centrifugal Partition Chromatography, Chromatographic Science Series, Vol. 68. Marcel Dekker, New York, Ito Y and Conway W D ( Eds. ) H igh - speed Countercurrent Chroma2 tography. Chem ical Analysis, Vol W iley, New York, W orld Science and Technology /M odernization of Traditional Chinese M edicine and M ateria M edica 57

5 2007 Vol. 9 No. 1 4 Menet J M and Thiebaut D. ( eds. ) Countercurrent Chromatography, Characterization of the solvent system used in countercurrent chromatog2 raphy, Marcel Dekker, Inc New York Basel, Berthod A ( ed. ) Countercurrent Chromatography. W ilson & W ilson s Comprehensive Analytical Chem istry. Vol. XXXV III. Elsevier :, :, Zhang T Y, Cao X L, Han X. J L iq Chromatogr Relat Technol, 2003, 26 (9&10) Schaufelberger D E. In: Ito Y and Conway W D ( Eds. ) H igh - speed Countercurrent Chromatography. Chem ical Analysis, Vol W iley, New York, , , 1 (2) ( HSCCC)., 2001, 23 (5) A lvi K A. In: Culter S J and Culter H G ( Eds. ), B ioactive Natural Products. CRC Press, Boca Raton, FL, Fregeau N L and R inehart K L. Isolation of marine natural p roducts by high - speed countercurrent chromatography. In: Ito Y, Conway W D ( eds. ) H igh - Speed Countercurrent Chromatography. A W iley - Inter2 science Publication, ,,,.. -, 2003, 5 (4) OKa H, Ikai Y, Kawamuta N, et al. D irect interfacing of high - speed countercurrent chromatography to Frit electron ionization, chem ical ioni2 zation, and fast atom bombardment mass spectrometry. Anal Chem, 1991, Kong Z R, R inehart K L, M ilberg R M, Conway W D. App lication of high - speed countercurrent chromatography/ electrosp ray ionization mass spectrometry ( HSCCC / ESIMS) L iq Chrom & Rel Technol, 1998, 21 (1&2) 65. in natural p roducts chem istry. J 17Graham A S, McConvey I F and Shering P J, An evaluation of the per2 formance of a preparative CCC machine for the separation of an active pharmaceutical ingredient. J L iq Chrom and Rel Technol, 2001, Sutherland I, Hawes D, Ignatova S, et al. Review of p rogress towards the industrial scale - up of CCC. J L iq Chrom & Rel, Technol, 2005, 28 (12&13) Oka H, Harada K, Ito Y, et al. J Chromatogr A, 1998, Ito Y, Ma Y. J Chromatogr A, 1996, Ito Y, Yang F Q, Fitze P, et al. Sp iral disk A ssembly for HSCCC: Col2 umn design and basic studies on chromatographic resolution and station2 ary phase. J L iq Chromatogr Relat Technol, 2003, Ito Y, Yang F Q, Fitze P, et al. Imp roved sp iral disk assembly for high - speed countercurrent chromatography. J Chromatogr A, 2003, HSCCC and Its Applica tion s in M ed ica l Research Cao X ueli (B eijing Key L aboratory of P lan t R esou rce R esearch and D evelopm ent, B eijing Technology and B usiness U n iversity, B eijing ) H igh2speed countercurrent chromatography, or HSCCC, is a continuous liquid2liquid partition chromatography de2 veloped in the 1980 s. In recent years, HSCCC has been w idely used in research and development of traditional Chinese medicine, biomedicine, natural p roducts, functional foods and cosm etics, including separating natural active compo2 nents from p lant resources, develop ing standard reference material of natural components, fingerp rint analysis of tradi2 tional Chinese m edicine, and selecting and screening new natural m edicines. A review is p resented in this paper, on HSCCC separation mechanism, instrum entation, operation, and its app lications in TCM research. Keywords: H igh2speed countercurrent chrom atography ( HSCCC), traditional Chinese medicines ( TCM ) ( :, :, :) 58 W orld Science and Technology /M odernization of Traditional Chinese M edicine and M ateria M edica