SCPC Systems for Liquid-Liquid Extraction

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Oswin Thompson
5 years ago
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SCPC Systems for Liquid-Liquid Extraction The SCPC systems are a combination of HPLC with the SCPC as separation column for the purification of active ingredients by liquid-liquid extraction or

The method belongs to the product range of column chromatography despite its column material free technology. Applications meet areas of e.g. pharmacognosy, pharmaceutical biology, organic chemistry, peptide chemistry, fermentations and soil ecology.

Inside the SCPC a rotor with many round stacked metal plates is located. Inside the rotor more than a thousand chambers interconnected in series.

The rotor is accelerated to about 2000 U / min, so that the centrifugal force causes the separation of the two liquid phases in the individual chambers.

1 SCPC Systems for Liquid-Liquid Extraction The SCPC systems are a combination of HPLC with the SCPC as separation column for the purification of active ingredients by liquid-liquid extraction or distribution chromatography. They are ideally suited for laboratory, pilot plant and production. Liquid-liquid extraction is also called liquid-liquid chromatography or distribution chromatography. The method belongs to the product range of column chromatography despite its column material free technology. Applications meet areas of e.g. pharmacognosy, pharmaceutical biology, organic chemistry, peptide chemistry, fermentations and soil ecology. For liquid-liquid extraction the SCPC is attached to HPLC as a rotary separation column instead of a HPLC column with a solid stationary phase. Inside the SCPC a rotor with many round stacked metal plates is located. Inside the rotor more than a thousand chambers interconnected in series. SCPC Rotor Rotor Chambers Distribution in the Chambers By means of the HPLC pump, the stationary liquid phase in the rotor is penetrated by a mobile liquid phase. The rotor is accelerated to about 2000 U / min, so that the centrifugal force causes the separation of the two liquid phases in the individual chambers. When the liquid mobile phase reaches equilibrium with the liquid stationary phase, the rotor is ready for sample injection. The separation process of the liquid-liquid extraction takes place within the two immiscible phases whereby the components of the sample are distributed within the two liquid phases according to their distribution coefficients. The components move so through the chambers at different rates to the rotor exit where they are fractionated. The combination of HPLC with the SCPC for liquid-liquid extraction allows separations of samples of various matrices in the analytical, preparative, and production scale. The SCPC systems are schematically identical regardless of their size and throughput. The central unit of each system is the SCPC. It virtually resembles the separation column surrounded by peripheral devices, such as HPLC pump, sample injector, UV-VIS detector, fraction collector and computer with HPLC software for control and chromatogram evaluation. 1/5

Example 1: SCPC System for Laboratory Pump Detector SCPC Fraction Collector Example 2: SCPC System for Pilot Plant and Production HPLC Skid 6L ATEX SCPC 5000 ATEX Key Features HP Injetion of varying

solvent consumption compared to HPLC with solid phase column Direct sample injection of e.g.

ATEX optional Without irreversible adsorption Ideal for samples of all polarities Pressure stable up to 100 bar Compared to solid-liquid chromatography, liquid-liquid extraction is trouble-less,

2 Example 1: SCPC System for Laboratory Pump Detector SCPC Fraction Collector Example 2: SCPC System for Pilot Plant and Production HPLC Skid 6L ATEX SCPC 5000 ATEX Key Features HP Injetion of varying sample volumes, low and high, at the same chromatographic conditions Super-fast chromatography runs without interfering matrix effects Cost-saving as no solid phase and only solvents necessary Less solvent consumption compared to HPLC with solid phase column Direct sample injection of e.g. crude extracts, without sample pretreatment Quick and loss-free separation of crude extracts Upscaling from milligram to kilogram scale One hundred percent recovery of the ingredients GMP, FDA and ATEX optional Without irreversible adsorption Ideal for samples of all polarities Pressure stable up to 100 bar Compared to solid-liquid chromatography, liquid-liquid extraction is trouble-less, simple and inexpensive, especially when crude extracts of different matrices have to be purified. The method does not directly compete with the solid-liquid HPLC, but rather accelerates in combination with HPLC the entire chromatographic process to the pure product. Further benefits of the liquid-liquid extraction using the SCPC is given in the great saving of time and money, placing it in general as an unparalleled innovative technology and particularly in contrast to the classical LC technologies. 2/5

3 Some Versions of SCPC 100 ml Rotor Volume 250 ml Rotor Volume 1000 ml Rotor Volume 5000 ml Rotor Volume There also are other versions of the SCPC. Just feel free to contact us, we will be very happy to answer your or phone call. Perhaps the following performance comparisons between LC / HPLC with solid phase column and LC / HPLC with SCPC are interesting for you. Liquid-Liquid Extraction Performance Comparison Solid Phase Column vs. SCPC Example 1: Production of a Plant-Derived Active Ingredient Column/Rotor Glascolumn ID 10 cm H 200 cm Rotor 6 Liters Sample volume 250 g crude extract, 44 runs p.a. = g 250 g crude extract, 110 runs p.a.= g Solvent per run Liters of Heptan per run 1:1 150 Liters Heptan/ACN Separation mat. Silicagel per year none Duration for 250 g crude extract 5 working days for 250 g crude extract 2 working days LC-System / R-System / Heptan Liters Heptan Liters Heptan Recycling Heptan Recycling ACN Liters ACN Recycling Separation material Separation material 0 per year per year per day per day 684 Total 5 days each / run days each / run /g / 250 g / 250 g 25 g/h 250 g / 5 days = 40 h 6,25 g 250 g / 2 days = 16 h 15,6 g 3/5

4 Example 2: Production of a Medical Dye Column/Rotor Glass Column ID 5 cm H 100 cm Rotor 1 Liter Sample volume 5 g sample, 55 runs p.a. = 275 g 5 g sample, 110 runs p.a. = 550 g Solvent per run 20 Liter MeOH per run 1,5 Liter BUOH/10L H 2O Separation mat. Silicagel per year none Duration for 5 g sample 4 working days for 5 g sample 2 working days LC-System /6 833 R-System / MeOH Liters BUOH 165 Liters H 2O Liters Separation material Separation material 0 per year per year per day 79 per day 142 Total 4 days each / run days each / run /g / 5 g / 5 g g/h 5 g / 4 days = 32 h 0,16 g 5 g / 2 days = 16 h 0,31 g Example 3: Production of Peptides Column/Rotor HPLC-Column ID 10 cm H 25 cm Rotor 6 Liters Sample volume 60 g sample, 220 runs p.a. = g 60 g sample, 880 runs p.a.= g Solvent per run 70:30 54 Liter ACN/H 2O per run 1:1 9 Liter BUOH/H 2O Separation mat. Silicagel per year none Duration for 60 g sample 8 hours for 60 g sample 2 hours HPLC-System / R-System / ACN Liters BUOH Liters BUOH Recycling H 2O Liters H 2O Liters Separation material Separation material 0 per year per year per day per day 488 Total 1 day each / run hours each 361 / run 722 /g / 60 g / 60 g 12 g/h 60 g / 1 day = 8 h 7,5 g 60 g / 2 h 30 g 4/5

5 Example 4: Product Purification after Organic Synthesis Column/Rotor HPLC-Column ID 10 cm H 25 cm Rotor 6 Liters Sample volume 60 g sample, 220 runs p.a. = g 60 g sample, 880 runs p.a.= g Solvent per run 90:10 per run 1:1 Separation mat. Silicagel per year none Duration for 60 g sample 8 hours for 60 g sample 2 hours HPLC-System / R-System / Heptan Liters Heptan Liters Heptan Recycling Heptan Recycling Ethylacetat Liters Ethylacetat Liters Ethylacetat Recycling Ethylacetat Recycling MeOH Liters MeOH Recycling H 2O Liters Separation material Separation material 0 per year per year per day 599 per day 456 Total 1 day each / run hours each 357 / run 714 /g / 60 g / 60 g 12 g/h 60 g / 1 day = 8 h 7,5 g 60 g / 2 h 30 g 5/5

Liquid-Liquid Extraction also known as distribution chromatography or liquid-liquid chromatography.

Liquid-Liquid Extraction also known as distribution chromatography or liquid-liquid chromatography. The separation of substance mixtures is often very difficult due to poor sample solubility, composition