CHEMTAX,. , CHEMTAX, g/m % % 4. 50% % 4. 83% %.

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1 29 2 Vol. 29, No JOURNAL OF OCEANOGRAPHY IN TA IWAN STRA IT May, , 2, 2 (1., ; 2., ) : (RP2HPLC) , CHEMTAX,. RP2HPLC, 1 /3,. RP2HPLC, , ( fucoxanthin) 19 2 ( 19 2butanoyloxyfucoxanthin) 19 2 (19 2hexanoyloxyfucoxanthin) (peridinin), g/m 3. CHEMTAX :,, % % 4. 50% % 4. 83% %. 2 6,.,. : ; ; ; DO I: /J. ISSN : P 735 : A : (2010) ,,,, [ 1 ].,,.,. (RP2HPLC),,,,., ( ) HPLC,. HPLC,,. CHEMTAX,, [ 2 ].,,,,.,, CHEMTAX HPLC., CHEMTAX,. : : ( ) ; ( ) ; ( ) : (1978 ),,, ; E2mail: hexuejia@hotmail. com : (1966 ),, ; E2mail: xypeng@ xmu. edu. cn

2 2 : , 7, 4 : (Bacillariophyceae) : ( Thalassiosira w eissfloyi) ; ( Prymnesiophycea) : ( Isochrysis ga lbana) ; (Chlorophycea) : (D una liella sp. ), (Ch lorella au totropica), ( P latym onas sp. ) ; (Cyanophyceae) : (Spirulina sp. ), (Synechococcus sp. ).. f/2, 20, lx, 12 12; 1. 2,, ( P < 266hPa) (W hatman GF /C, = 47mm, Cat. No ). 1cm 3,,, ( 1) 3d. 5dm dm 3, 130 m,. 1. 4, 5cm 3, 2Apo28 carotenol ( trans) ( Sigma2A ldrich, USA ), 5cm 3, 3 5m in, ( - 20 ) 24h. (W hatman GF /C, = 24mm) ( P < 266hPa), ( 2. 0 cm 3 )., HPLC HPLC Agilent HP1100 Series, Hewlett Packard, (DAD ) 440nm nm. : A: 0. 5mol/dm 3 = (V /V, ph 7. 2) ; B: = (V /V ). : 16m in, A B, 8m in, DAD CHEMTAX (CHEMTAX 1. 95), ( D inophyceae ), ( Bacillariophyceae ), ( Prymnesiophycea ), (Chlorophyta) (Cyanophyceae). (1999) [ 3 ] Mackey(1996) [ 2 ].

3 [ 2 2Apo28 carotenol ( trans) ] ( 1)., ( cis2fucoxanthin) a. a ( chlorophyllide a) b ( chlorophyllide b), ( Prasinophyceae) (Cryp tophyceae) (p rasinoxanthin) ( alloxanthin),,.,, ( TLC) (HPTLC), HPLC,,,,.,. HPLC (1999) [ 3 ] Nelson (1993) [ 4 ], A, 1 /3 ( 36m in 24m in), 18,,.,,,. 1 HPLC DAD Tab. 1 Pigments separated and identified using HPLC with characteristics measured using DAD /m in / nm 1 chlorophyll C1 + C C1 + C 2 445, 440, 469 CHL C1 + C 2 2 peridine , 468 PER ID butanoyloxyfucoxanthin , 467 BUT2FUCO 4 fucooxanthin , 468 FUCO 5 neoxanthin , 440, 469 NEO 6 violaxanthin , 441, 470 V IOLA hexanoyloxyfucoxanthin , 476 HEX2FUCO 8 cis2fucooxanthin cis2 446, 476 cis2fuco 9 diadinoxanthin , 465 D IAD INO 10 alloxanthin , 446, 473 ALLO 11 diatoxanthin , 442, 470 D IATO 12 zeaxanthin , 479 ZEA 13 lutein , 469 LUT b2apo28 carotenol( trans) chlorophyll b b 446, 650 CHL b 15 chlorophyll a ( ep imer) a ( ) 432, 666 CHL a EP IM 16 chlorophyll a a 430, 666 CHL a 17 chlorophyll a ( allomer) a ( ) 430, 665 CHL a ALLOM 18 2carotene , 476 2CAR : 3

4 2 : HPLC, 18, ( zeanxanthin), 2., ( fucoxanthin) 19 2 ( 19 2butanoyloxyfucoxanthin) 19 2 ( 19 2 hexanoyloxyfucoxanthin) (peridinin), g/m 3., 100 g/m CHEMTAX, a ( 3), ( 2) a g/m 3, (2003) [ 5 ]. 2, % %,, 4. 50% % 4. 83% %, 6. 84% %,. [ 5-6 ], 3 (53. 97% ) : % % % [ 6 ],,,. (2003) [ 5 ],,,.,, ( Prasinophyceae) (Cryp tophyceae),,., , %, %,, 6. a ( 2), 5,. 5 ( g/m 3 ). (1990) 6 [ 7 ],., Tab. 2 Concentrations of diagnostic carotenoids, chlorophyll and carotene in western Xiamen waters from February to June, 2001 / g m PER BUT2FUCO HEX FUCO NEO V IOLA D IAD INO D IATO ZEA LUT CHL b CAR CHL a : 2 9, 10

5 a Tab. 3 B iomass ( chlorophyll a) of major phytop lankton group s of southern Xiamen waters from February to June, 2001 a / g m ,, ( 3),,,.,,.,,,. 25 ( 3),, mg/dm 3, mg/dm 3, 4,,,, ( ),,.,, 6. 3 HPLC, HPLC, , CHEMTAX ,,, % %,.,.

6 2 : : [ 1 ] FawleyM W, Lee C M. Pigment composition of the scaly green flagellate M esostigm a viride (M icromonadophyceae) is sim ilar to that of the siphonous green alga B ryopsis plum ose (U lvophyceae) [ J ]. Journal of Phycology, 1990, 26: [ 2 ] MackeyM D,Mackey D J, H iggins H W, et al. CHEMTAS2a p rogram for estimating chlass abundances from chem ical markers: app lication to HPLC measurements of phytop lankton[ J ]. Marine Ecology Progress Series, 1996, 144: [ 3 ],,. [ J ]., 1999, 124 (4) : 629. [ 4 ] Nelson J R. Rates and possible mechanism of light2dependent degradation of p igments in detritus derived from phytop lankton [ J ]. Journal of M arine Research, 1993, 51: [ 5 ],,,. [ J ]., 2003, 22 (3) : [ 6 ],.. [ J ]., 2000, 22 (3) : [ 7 ]. [ J ]., 1990, 10 (2) : Phytop lankton comm unity structure in we ste rn Xiam en wa te rs from Februa ry to J une 2001 from ana lysis of pho to synthe tic p igm ents HE Xue2jia 1, GAO Ya2hui 2, PENG Xing2yue 2 ( 1. Research Center for Harmful A lgae and Aquatic Environment, J inan University, Guangzhou , China; 2. School of L ife Sciences, Xiamen University, Xiamen , China) Ab s tra c t: Photosynthetic p igments were quantified, using RP2HPLC, from phytop lankton samp les collected in western Xiamen waters from February to June in D iagnostic p igment data were converted into chlorophyll a biomass values to assess phytop lankton comm unity structure using CHEM TAX software. The effects of environm ental factors on phytop lankton comm unity were also analyzed. emp loyed which consistently reduced the separating tim e by a third. An imp roved RP2HPLC p rotocol was The separation of p igments using the imp roved RP2HPLC p rotocol suggested that the p rime diagnostic p igments in this area during the investigation were Fucoxanthin, 19 2butanoyloxyfucoxanthin, 19 2hexanoyloxyfucoxanthin and peridinin w ith average monthly concentrations varying in the following ranges: , , and g/m 3, respectively. CHEMTAX calculations demonstrated that the dom inant group was diatom s w ith a contribution of % to % to total phytop lankton biom ass, followed by p rym nesiophytes and dinophytes, which accounted for 4. 50% % and 4. 83% %, respectively. Total phytop lankton biomass increased from February to June, in response to the continuously increasing temperature. The relative contribution of diatom s varied in a trend opposite to that of p rymnesiophytes, competition was an important factor for phytop lankton community structure in this area. suggesting that inter2species Ke y wo rd s: marine biology; diagnostic p igm ents; phytop lankton community structure; western Xiam en waters DO I: /J. ISSN ( : )